Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063148.t1 | XP_003603961.1 | 94.9 | 369 | 18 | 1 | 1 | 368 | 1 | 369 | 4.80E-198 | 700.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063148.t1 | Q9AVK1 | 45.1 | 381 | 193 | 5 | 1 | 367 | 1 | 379 | 3.3e-86 | 320.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063148.t1 | G7J763 | 94.9 | 369 | 18 | 1 | 1 | 368 | 1 | 369 | 3.5e-198 | 700.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04932 | MS.gene063148 | 0.807707 | 4.43E-50 | -1.69E-46 |
| MS.gene049549 | MS.gene063148 | 0.803769 | 2.98E-49 | -1.69E-46 |
| MS.gene050344 | MS.gene063148 | 0.800006 | 1.76E-48 | -1.69E-46 |
| MS.gene051044 | MS.gene063148 | 0.833183 | 6.17E-56 | -1.69E-46 |
| MS.gene05104 | MS.gene063148 | 0.804135 | 2.50E-49 | -1.69E-46 |
| MS.gene051127 | MS.gene063148 | 0.804347 | 2.26E-49 | -1.69E-46 |
| MS.gene051138 | MS.gene063148 | 0.823127 | 1.64E-53 | -1.69E-46 |
| MS.gene051439 | MS.gene063148 | 0.832748 | 7.92E-56 | -1.69E-46 |
| MS.gene051440 | MS.gene063148 | 0.827567 | 1.46E-54 | -1.69E-46 |
| MS.gene051517 | MS.gene063148 | 0.844825 | 5.95E-59 | -1.69E-46 |
| MS.gene051973 | MS.gene063148 | 0.82723 | 1.75E-54 | -1.69E-46 |
| MS.gene052211 | MS.gene063148 | 0.804824 | 1.79E-49 | -1.69E-46 |
| MS.gene05240 | MS.gene063148 | 0.807036 | 6.15E-50 | -1.69E-46 |
| MS.gene052606 | MS.gene063148 | 0.818091 | 2.35E-52 | -1.69E-46 |
| MS.gene052702 | MS.gene063148 | 0.802411 | 5.68E-49 | -1.69E-46 |
| MS.gene052703 | MS.gene063148 | 0.815761 | 7.83E-52 | -1.69E-46 |
| MS.gene053097 | MS.gene063148 | 0.800406 | 1.46E-48 | -1.69E-46 |
| MS.gene053887 | MS.gene063148 | 0.821277 | 4.40E-53 | -1.69E-46 |
| MS.gene054277 | MS.gene063148 | 0.821732 | 3.45E-53 | -1.69E-46 |
| MS.gene054742 | MS.gene063148 | 0.803495 | 3.39E-49 | -1.69E-46 |
| MS.gene056224 | MS.gene063148 | 0.82788 | 1.22E-54 | -1.69E-46 |
| MS.gene056590 | MS.gene063148 | 0.819816 | 9.52E-53 | -1.69E-46 |
| MS.gene056591 | MS.gene063148 | 0.817221 | 3.69E-52 | -1.69E-46 |
| MS.gene056697 | MS.gene063148 | 0.814625 | 1.40E-51 | -1.69E-46 |
| MS.gene056748 | MS.gene063148 | 0.83773 | 4.37E-57 | -1.69E-46 |
| MS.gene056856 | MS.gene063148 | 0.84835 | 6.48E-60 | -1.69E-46 |
| MS.gene056920 | MS.gene063148 | 0.815273 | 1.01E-51 | -1.69E-46 |
| MS.gene056988 | MS.gene063148 | 0.83288 | 7.34E-56 | -1.69E-46 |
| MS.gene057261 | MS.gene063148 | 0.809179 | 2.15E-50 | -1.69E-46 |
| MS.gene057548 | MS.gene063148 | 0.825876 | 3.69E-54 | -1.69E-46 |
| MS.gene057550 | MS.gene063148 | 0.801085 | 1.06E-48 | -1.69E-46 |
| MS.gene057717 | MS.gene063148 | 0.809213 | 2.11E-50 | -1.69E-46 |
| MS.gene057768 | MS.gene063148 | 0.820403 | 6.99E-53 | -1.69E-46 |
| MS.gene058068 | MS.gene063148 | 0.805537 | 1.27E-49 | -1.69E-46 |
| MS.gene058099 | MS.gene063148 | 0.814212 | 1.73E-51 | -1.69E-46 |
| MS.gene058334 | MS.gene063148 | 0.80054 | 1.37E-48 | -1.69E-46 |
| MS.gene058381 | MS.gene063148 | 0.817637 | 2.97E-52 | -1.69E-46 |
| MS.gene058452 | MS.gene063148 | 0.815346 | 9.69E-52 | -1.69E-46 |
| MS.gene058603 | MS.gene063148 | 0.832496 | 9.15E-56 | -1.69E-46 |
| MS.gene05926 | MS.gene063148 | 0.810336 | 1.21E-50 | -1.69E-46 |
| MS.gene059304 | MS.gene063148 | 0.811652 | 6.29E-51 | -1.69E-46 |
| MS.gene059315 | MS.gene063148 | 0.80333 | 3.67E-49 | -1.69E-46 |
| MS.gene060087 | MS.gene063148 | 0.835291 | 1.83E-56 | -1.69E-46 |
| MS.gene060405 | MS.gene063148 | 0.854621 | 1.09E-61 | -1.69E-46 |
| MS.gene061044 | MS.gene063148 | 0.841788 | 3.84E-58 | -1.69E-46 |
| MS.gene061094 | MS.gene063148 | 0.804231 | 2.39E-49 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063148.t1 | MTR_3g117060 | 94.851 | 369 | 18 | 1 | 1 | 368 | 1 | 369 | 0.0 | 728 |
| MS.gene063148.t1 | MTR_3g467560 | 53.678 | 367 | 165 | 3 | 1 | 366 | 25 | 387 | 3.33e-138 | 399 |
| MS.gene063148.t1 | MTR_7g084620 | 51.762 | 369 | 169 | 5 | 1 | 366 | 1 | 363 | 1.71e-130 | 378 |
| MS.gene063148.t1 | MTR_7g084350 | 50.409 | 367 | 175 | 4 | 3 | 366 | 1 | 363 | 2.66e-128 | 373 |
| MS.gene063148.t1 | MTR_8g077040 | 45.187 | 374 | 191 | 4 | 1 | 366 | 1 | 368 | 3.52e-114 | 337 |
| MS.gene063148.t1 | MTR_4g018820 | 46.257 | 374 | 180 | 4 | 1 | 368 | 1 | 359 | 7.69e-114 | 335 |
| MS.gene063148.t1 | MTR_5g020940 | 45.312 | 384 | 181 | 6 | 1 | 368 | 11 | 381 | 1.01e-112 | 333 |
| MS.gene063148.t1 | MTR_3g467270 | 45.867 | 375 | 188 | 6 | 1 | 367 | 1 | 368 | 1.32e-112 | 333 |
| MS.gene063148.t1 | MTR_8g076900 | 44.086 | 372 | 194 | 3 | 1 | 368 | 1 | 362 | 1.53e-109 | 325 |
| MS.gene063148.t1 | MTR_8g020690 | 43.432 | 373 | 193 | 5 | 1 | 368 | 1 | 360 | 3.94e-105 | 313 |
| MS.gene063148.t1 | MTR_6g056070 | 43.548 | 372 | 189 | 5 | 1 | 367 | 1 | 356 | 2.25e-104 | 311 |
| MS.gene063148.t1 | MTR_8g020700 | 41.223 | 376 | 194 | 4 | 1 | 368 | 1 | 357 | 8.26e-99 | 297 |
| MS.gene063148.t1 | MTR_7g084330 | 45.821 | 347 | 160 | 7 | 25 | 366 | 8 | 331 | 3.36e-96 | 290 |
| MS.gene063148.t1 | MTR_5g046110 | 42.486 | 346 | 179 | 4 | 1 | 339 | 61 | 393 | 8.48e-96 | 291 |
| MS.gene063148.t1 | MTR_1g022465 | 43.717 | 382 | 195 | 7 | 1 | 367 | 1 | 377 | 1.09e-91 | 280 |
| MS.gene063148.t1 | MTR_1g022455 | 42.408 | 382 | 201 | 7 | 1 | 367 | 1 | 378 | 6.90e-89 | 273 |
| MS.gene063148.t1 | MTR_8g076900 | 51.200 | 250 | 113 | 2 | 1 | 246 | 1 | 245 | 5.36e-86 | 261 |
| MS.gene063148.t1 | MTR_6g015620 | 95.122 | 123 | 6 | 0 | 246 | 368 | 16 | 138 | 3.01e-78 | 237 |
| MS.gene063148.t1 | MTR_8g020700 | 45.769 | 260 | 131 | 2 | 1 | 255 | 1 | 255 | 5.71e-77 | 238 |
| MS.gene063148.t1 | MTR_3g054330 | 35.450 | 378 | 220 | 12 | 1 | 365 | 10 | 376 | 2.26e-62 | 204 |
| MS.gene063148.t1 | MTR_8g077030 | 36.735 | 343 | 127 | 6 | 1 | 342 | 1 | 254 | 2.24e-59 | 200 |
| MS.gene063148.t1 | MTR_5g093900 | 32.552 | 384 | 233 | 9 | 1 | 365 | 13 | 389 | 8.76e-58 | 192 |
| MS.gene063148.t1 | MTR_3g052700 | 32.984 | 382 | 232 | 10 | 1 | 365 | 13 | 387 | 1.32e-57 | 192 |
| MS.gene063148.t1 | MTR_1g108660 | 32.378 | 349 | 195 | 8 | 26 | 361 | 9 | 329 | 1.92e-52 | 177 |
| MS.gene063148.t1 | MTR_7g084340 | 43.802 | 121 | 63 | 2 | 248 | 366 | 12 | 129 | 8.94e-23 | 92.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063148.t1 | AT5G38020 | 44.663 | 356 | 178 | 6 | 6 | 350 | 3 | 350 | 9.16e-96 | 290 |
| MS.gene063148.t1 | AT3G11480 | 42.133 | 375 | 189 | 6 | 2 | 367 | 24 | 379 | 2.20e-92 | 281 |
| MS.gene063148.t1 | AT4G36470 | 42.319 | 345 | 183 | 5 | 3 | 339 | 6 | 342 | 3.51e-90 | 276 |
| MS.gene063148.t1 | AT5G66430 | 40.984 | 366 | 197 | 6 | 9 | 367 | 1 | 354 | 6.98e-90 | 274 |
| MS.gene063148.t1 | AT3G21950 | 43.266 | 349 | 177 | 7 | 6 | 342 | 3 | 342 | 1.09e-87 | 269 |
| MS.gene063148.t1 | AT5G38020 | 45.687 | 313 | 152 | 5 | 48 | 350 | 7 | 311 | 4.32e-86 | 264 |
| MS.gene063148.t1 | AT1G19640 | 40.665 | 391 | 203 | 7 | 1 | 366 | 1 | 387 | 1.67e-84 | 262 |
| MS.gene063148.t1 | AT5G04370 | 39.730 | 370 | 196 | 7 | 7 | 367 | 39 | 390 | 9.80e-84 | 260 |
| MS.gene063148.t1 | AT5G04370 | 39.730 | 370 | 196 | 7 | 7 | 367 | 58 | 409 | 1.20e-83 | 260 |
| MS.gene063148.t1 | AT5G04370 | 39.730 | 370 | 196 | 7 | 7 | 367 | 44 | 395 | 1.41e-83 | 259 |
| MS.gene063148.t1 | AT3G21950 | 39.895 | 381 | 176 | 8 | 6 | 342 | 3 | 374 | 4.32e-81 | 253 |
| MS.gene063148.t1 | AT5G04380 | 40.346 | 347 | 186 | 6 | 7 | 346 | 36 | 368 | 9.28e-80 | 249 |
| MS.gene063148.t1 | AT2G14060 | 40.113 | 354 | 193 | 8 | 7 | 352 | 12 | 354 | 4.63e-76 | 239 |
| MS.gene063148.t1 | AT5G04380 | 39.481 | 347 | 182 | 7 | 7 | 346 | 32 | 357 | 2.05e-75 | 238 |
| MS.gene063148.t1 | AT5G04380 | 41.639 | 305 | 158 | 5 | 48 | 346 | 7 | 297 | 9.87e-75 | 234 |
| MS.gene063148.t1 | AT5G55250 | 34.819 | 359 | 211 | 9 | 1 | 345 | 13 | 362 | 8.96e-61 | 200 |
| MS.gene063148.t1 | AT2G14060 | 45.333 | 225 | 114 | 3 | 6 | 229 | 11 | 227 | 3.64e-58 | 189 |
| MS.gene063148.t1 | AT1G15125 | 33.894 | 357 | 184 | 11 | 9 | 346 | 1 | 324 | 4.84e-57 | 189 |
| MS.gene063148.t1 | AT1G68040 | 32.787 | 366 | 209 | 10 | 3 | 356 | 7 | 347 | 9.30e-55 | 184 |
| MS.gene063148.t1 | AT5G38100 | 33.523 | 352 | 195 | 8 | 9 | 346 | 10 | 336 | 1.27e-51 | 176 |
| MS.gene063148.t1 | AT5G37970 | 33.333 | 363 | 196 | 13 | 5 | 348 | 6 | 341 | 3.05e-51 | 175 |
| MS.gene063148.t1 | AT5G55250 | 33.742 | 326 | 193 | 9 | 34 | 345 | 8 | 324 | 9.62e-51 | 173 |
| MS.gene063148.t1 | AT3G44840 | 33.140 | 344 | 197 | 8 | 9 | 345 | 7 | 324 | 1.96e-50 | 172 |
| MS.gene063148.t1 | AT5G37990 | 33.333 | 363 | 196 | 12 | 5 | 348 | 6 | 341 | 5.84e-50 | 171 |
| MS.gene063148.t1 | AT3G44870 | 37.413 | 286 | 156 | 8 | 9 | 287 | 7 | 276 | 6.41e-49 | 169 |
| MS.gene063148.t1 | AT5G38100 | 34.868 | 304 | 166 | 6 | 52 | 346 | 33 | 313 | 4.68e-48 | 166 |
| MS.gene063148.t1 | AT5G56300 | 30.110 | 362 | 222 | 10 | 2 | 346 | 17 | 364 | 2.58e-47 | 165 |
| MS.gene063148.t1 | AT5G38780 | 31.564 | 358 | 204 | 11 | 5 | 346 | 6 | 338 | 3.82e-47 | 164 |
| MS.gene063148.t1 | AT3G44860 | 36.364 | 286 | 162 | 7 | 9 | 287 | 7 | 279 | 8.59e-47 | 163 |
| MS.gene063148.t1 | AT1G66700 | 35.739 | 291 | 160 | 9 | 9 | 287 | 9 | 284 | 7.78e-46 | 160 |
| MS.gene063148.t1 | AT1G66720 | 32.653 | 343 | 190 | 11 | 9 | 339 | 9 | 322 | 1.53e-43 | 154 |
| MS.gene063148.t1 | AT5G38780 | 33.443 | 305 | 166 | 10 | 54 | 346 | 10 | 289 | 1.72e-43 | 153 |
| MS.gene063148.t1 | AT4G26420 | 29.248 | 359 | 222 | 10 | 2 | 342 | 6 | 350 | 2.45e-41 | 149 |
| MS.gene063148.t1 | AT1G66690 | 32.425 | 367 | 208 | 11 | 1 | 353 | 1 | 341 | 2.81e-40 | 145 |
| MS.gene063148.t1 | AT1G15125 | 36.538 | 260 | 125 | 9 | 9 | 249 | 1 | 239 | 6.01e-39 | 139 |
| MS.gene063148.t1 | AT5G38100 | 36.800 | 250 | 132 | 6 | 9 | 244 | 10 | 247 | 7.70e-38 | 136 |
| MS.gene063148.t1 | AT5G38100 | 36.800 | 250 | 132 | 6 | 9 | 244 | 10 | 247 | 7.87e-38 | 136 |
| MS.gene063148.t1 | AT1G66700 | 36.279 | 215 | 119 | 5 | 9 | 218 | 9 | 210 | 2.29e-34 | 127 |
| MS.gene063148.t1 | AT4G26420 | 32.018 | 228 | 140 | 6 | 2 | 223 | 6 | 224 | 1.69e-29 | 114 |
| MS.gene063148.t1 | AT4G26420 | 31.674 | 221 | 136 | 6 | 9 | 223 | 1 | 212 | 3.48e-27 | 108 |
Find 68 sgRNAs with CRISPR-Local
Find 134 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATAAGATTATTAAACTTTA+TGG | 0.051266 | 3.4:+99543874 | MS.gene063148:CDS |
| TGCAAATAACTCCACATTTC+AGG | 0.197207 | 3.4:+99541627 | MS.gene063148:CDS |
| TCAGCCACTTTCAAACAATT+TGG | 0.225467 | 3.4:-99541961 | None:intergenic |
| ACTTTATGGAGTTGAGAAAT+TGG | 0.267027 | 3.4:+99543888 | MS.gene063148:CDS |
| TAATGGATGAATTGTTCATT+AGG | 0.276319 | 3.4:+99543845 | MS.gene063148:CDS |
| ATCAGGTCCAAATGCACTTT+TGG | 0.294357 | 3.4:+99541996 | MS.gene063148:CDS |
| ATTGGTGGAGCAATCAAAAT+TGG | 0.310300 | 3.4:+99543585 | MS.gene063148:CDS |
| GCAATGGTCCTAACACTTAT+TGG | 0.313733 | 3.4:+99543089 | MS.gene063148:CDS |
| TTTAGTCCATGTTTCTTTAG+TGG | 0.317085 | 3.4:+99542186 | MS.gene063148:CDS |
| CGTTCGTCTGAACTGCTTCC+TGG | 0.322621 | 3.4:+99543062 | MS.gene063148:CDS |
| GATGCAACCAAAAGTGCATT+TGG | 0.356622 | 3.4:-99542003 | None:intergenic |
| AGAGTTTGGAGAAGAAATAA+TGG | 0.384468 | 3.4:+99543828 | MS.gene063148:CDS |
| CTGGAGGGCTTGCCCTTGTT+AGG | 0.391432 | 3.4:-99542980 | None:intergenic |
| TAAATATTCCCTTTGTTCAA+TGG | 0.392424 | 3.4:-99542957 | None:intergenic |
| CATCTCTGCCAATAAGTGTT+AGG | 0.402508 | 3.4:-99543097 | None:intergenic |
| TATTGAAGTCATTTCCAAAT+AGG | 0.408776 | 3.4:-99542104 | None:intergenic |
| ATAAATGCATGGGTTGTCAT+TGG | 0.420457 | 3.4:+99543134 | MS.gene063148:CDS |
| AAAGCATCTTGCAACACATC+TGG | 0.424458 | 3.4:-99542930 | None:intergenic |
| GCTGACTTAGGTTGTTCATC+AGG | 0.433358 | 3.4:+99541979 | MS.gene063148:CDS |
| CTCCTCAGACGTAGGGCAAT+AGG | 0.442711 | 3.4:-99543626 | None:intergenic |
| AGAAAAGTGATTGAAGAAGA+AGG | 0.455276 | 3.4:+99543652 | MS.gene063148:CDS |
| AGAGACAATTAGAACGGATT+GGG | 0.455968 | 3.4:+99543696 | MS.gene063148:CDS |
| CTTTAGAACCATTGAACAAA+GGG | 0.461138 | 3.4:+99542949 | MS.gene063148:CDS |
| GGAAAGCCAATGTAGACTAT+AGG | 0.465513 | 3.4:-99542271 | None:intergenic |
| TAGAGACAATTAGAACGGAT+TGG | 0.473214 | 3.4:+99543695 | MS.gene063148:CDS |
| GAAAAGTGATTGAAGAAGAA+GGG | 0.480442 | 3.4:+99543653 | MS.gene063148:CDS |
| TTGGTATATGCAGAAATTGG+TGG | 0.497369 | 3.4:+99543570 | MS.gene063148:intron |
| TTTCTAATCTCCTCAGACGT+AGG | 0.497948 | 3.4:-99543634 | None:intergenic |
| TGTTTCTTTAGTGGAACACC+TGG | 0.509820 | 3.4:+99542195 | MS.gene063148:CDS |
| AAAATGAACTTATAAATGCA+TGG | 0.515846 | 3.4:+99543123 | MS.gene063148:CDS |
| ATTCCTCCTATAGTCTACAT+TGG | 0.529876 | 3.4:+99542265 | MS.gene063148:CDS |
| ACTGCTTCCTGGTGGTGCAA+TGG | 0.531373 | 3.4:+99543073 | MS.gene063148:CDS |
| GAACAAGTCCTTCACATGAA+TGG | 0.532956 | 3.4:+99541583 | MS.gene063148:CDS |
| TTCTAATCTCCTCAGACGTA+GGG | 0.536749 | 3.4:-99543633 | None:intergenic |
| AAGGGAATATTTACCTAACA+AGG | 0.540815 | 3.4:+99542967 | MS.gene063148:CDS |
| TGTTTGAAAGTGGCTGACTT+AGG | 0.547633 | 3.4:+99541967 | MS.gene063148:CDS |
| TAGTCTACATTGGCTTTCCA+AGG | 0.551384 | 3.4:+99542275 | MS.gene063148:CDS |
| AAATGAACTTATAAATGCAT+GGG | 0.553184 | 3.4:+99543124 | MS.gene063148:CDS |
| ATCAGGGAGTAACTTAAAAG+TGG | 0.559627 | 3.4:-99542127 | None:intergenic |
| ACTGAAACATGGGTGACTCA+TGG | 0.560015 | 3.4:-99542068 | None:intergenic |
| TTATGGAGTTGAGAAATTGG+AGG | 0.566069 | 3.4:+99543891 | MS.gene063148:CDS |
| AAGCCAATGTAGACTATAGG+AGG | 0.570549 | 3.4:-99542268 | None:intergenic |
| CTTCACATGAATGGAGGTGA+GGG | 0.570634 | 3.4:+99541592 | MS.gene063148:CDS |
| AAGAGACTACAAGAAGAGAA+AGG | 0.570921 | 3.4:+99542156 | MS.gene063148:CDS |
| AAGGTTAGAGACAATTAGAA+CGG | 0.575103 | 3.4:+99543690 | MS.gene063148:CDS |
| ATTAATCATTACCTGAAATG+TGG | 0.578791 | 3.4:-99541638 | None:intergenic |
| ATCCTATTGCCCTACGTCTG+AGG | 0.583567 | 3.4:+99543624 | MS.gene063148:CDS |
| CATTTCCAAATAGGTCATTG+AGG | 0.588169 | 3.4:-99542095 | None:intergenic |
| CCTCACCTCCATTCATGTGA+AGG | 0.588787 | 3.4:-99541591 | None:intergenic |
| GTTAGGACCATTGCACCACC+AGG | 0.591625 | 3.4:-99543080 | None:intergenic |
| GCTTTAGAACCATTGAACAA+AGG | 0.600431 | 3.4:+99542948 | MS.gene063148:CDS |
| AACAAGGGCAAGCCCTCCAG+CGG | 0.613717 | 3.4:+99542983 | MS.gene063148:CDS |
| GTTTCTTTAGTGGAACACCT+GGG | 0.617366 | 3.4:+99542196 | MS.gene063148:CDS |
| CCTTCACATGAATGGAGGTG+AGG | 0.617516 | 3.4:+99541591 | MS.gene063148:CDS |
| TACTTGAAGATAGTATAATG+AGG | 0.631232 | 3.4:+99541920 | MS.gene063148:CDS |
| ACTCAATGACATGGCCGCAG+AGG | 0.644339 | 3.4:+99543163 | MS.gene063148:CDS |
| TGGCATGGCACTCAATGACA+TGG | 0.644988 | 3.4:+99543154 | MS.gene063148:CDS |
| AAAAGTGAATGGAATTGTCG+GGG | 0.649123 | 3.4:-99542239 | None:intergenic |
| GCAGAGAATGGTGATGCTGA+CGG | 0.652786 | 3.4:+99541888 | MS.gene063148:intron |
| CAAGTCCTTCACATGAATGG+AGG | 0.653986 | 3.4:+99541586 | MS.gene063148:CDS |
| GGTGTTCCACTAAAGAAACA+TGG | 0.655731 | 3.4:-99542192 | None:intergenic |
| TGCATGGGTTGTCATTGGCA+TGG | 0.666196 | 3.4:+99543139 | MS.gene063148:CDS |
| AGGGAATATTTACCTAACAA+GGG | 0.666854 | 3.4:+99542968 | MS.gene063148:CDS |
| TCAGACGTAGGGCAATAGGA+TGG | 0.668240 | 3.4:-99543622 | None:intergenic |
| ATGTTGTTTGATTACCTCTG+CGG | 0.673521 | 3.4:-99543177 | None:intergenic |
| GATGAAGACACAAGAGCTGA+GGG | 0.692498 | 3.4:+99543760 | MS.gene063148:CDS |
| TCGTCTGAACTGCTTCCTGG+TGG | 0.698405 | 3.4:+99543065 | MS.gene063148:CDS |
| TGATGAAGACACAAGAGCTG+AGG | 0.700921 | 3.4:+99543759 | MS.gene063148:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTATTTTAAAAAAAATT+AGG | - | chr3.4:99542589-99542608 | None:intergenic | 0.0% |
| !! | AAATAAAAAGTTGTAATTAA+TGG | + | chr3.4:99542600-99542619 | MS.gene063148:intron | 10.0% |
| !! | AAATTAATTAAACATTAACA+GGG | - | chr3.4:99542899-99542918 | None:intergenic | 10.0% |
| !! | AATAAGATTATTAAACTTTA+TGG | + | chr3.4:99543874-99543893 | MS.gene063148:CDS | 10.0% |
| !! | TATCATTATACTAATTAATT+TGG | + | chr3.4:99543420-99543439 | MS.gene063148:intron | 10.0% |
| !! | TTAAAAAAATTTATATAAGC+GGG | - | chr3.4:99543305-99543324 | None:intergenic | 10.0% |
| !!! | TATGTATGTATTATTTTATT+TGG | + | chr3.4:99543551-99543570 | MS.gene063148:intron | 10.0% |
| !!! | TTTTAAAATATTTTTTATCG+CGG | - | chr3.4:99542439-99542458 | None:intergenic | 10.0% |
| !! | AATTAATTAAACATTAACAG+GGG | - | chr3.4:99542898-99542917 | None:intergenic | 15.0% |
| !! | CAAATTAATTAAACATTAAC+AGG | - | chr3.4:99542900-99542919 | None:intergenic | 15.0% |
| !! | CTGTTAATGTTTAATTAATT+TGG | + | chr3.4:99542898-99542917 | MS.gene063148:intron | 15.0% |
| !!! | AATGATATTTTGATAATTGT+GGG | - | chr3.4:99543407-99543426 | None:intergenic | 15.0% |
| !!! | TAATGATATTTTGATAATTG+TGG | - | chr3.4:99543408-99543427 | None:intergenic | 15.0% |
| !! | AAAAAAATTGTTGCAACAAT+TGG | + | chr3.4:99542530-99542549 | MS.gene063148:intron | 20.0% |
| !! | AAAATGAACTTATAAATGCA+TGG | + | chr3.4:99543123-99543142 | MS.gene063148:CDS | 20.0% |
| !! | AAATAAGCACAAAATAACTT+CGG | - | chr3.4:99542415-99542434 | None:intergenic | 20.0% |
| !! | AAATGAACTTATAAATGCAT+GGG | + | chr3.4:99543124-99543143 | MS.gene063148:CDS | 20.0% |
| !! | AAATTATCTGCAAATATGAT+TGG | - | chr3.4:99542698-99542717 | None:intergenic | 20.0% |
| !! | ACTCACTTTGTAAATAATTA+AGG | - | chr3.4:99543473-99543492 | None:intergenic | 20.0% |
| !! | CGATATCATATACTATATTA+TGG | - | chr3.4:99542846-99542865 | None:intergenic | 20.0% |
| !!! | ACATACATATTTTTTTTTCG+TGG | - | chr3.4:99542805-99542824 | None:intergenic | 20.0% |
| !!! | ATGATATTTTGATAATTGTG+GGG | - | chr3.4:99543406-99543425 | None:intergenic | 20.0% |
| !!! | TTCGTGGTATAATAATTTTT+TGG | - | chr3.4:99542789-99542808 | None:intergenic | 20.0% |
| ! | AAAAAAGTGAATGGAATTGT+CGG | - | chr3.4:99542244-99542263 | None:intergenic | 25.0% |
| ! | ATGCAAATTCTACAAAACAA+TGG | - | chr3.4:99541708-99541727 | None:intergenic | 25.0% |
| ! | ATTAATCATTACCTGAAATG+TGG | - | chr3.4:99541641-99541660 | None:intergenic | 25.0% |
| ! | GTAGTCTCTTATAAAAATCA+GGG | - | chr3.4:99542146-99542165 | None:intergenic | 25.0% |
| ! | TAAATATTCCCTTTGTTCAA+TGG | - | chr3.4:99542960-99542979 | None:intergenic | 25.0% |
| ! | TACTTGAAGATAGTATAATG+AGG | + | chr3.4:99541920-99541939 | MS.gene063148:CDS | 25.0% |
| ! | TATTGAAGTCATTTCCAAAT+AGG | - | chr3.4:99542107-99542126 | None:intergenic | 25.0% |
| ! | TATTTGGTATATGCAGAAAT+TGG | + | chr3.4:99543567-99543586 | MS.gene063148:intron | 25.0% |
| ! | TGATGTCAATATATATATCC+TGG | + | chr3.4:99543447-99543466 | MS.gene063148:intron | 25.0% |
| ! | TGTAGTCTCTTATAAAAATC+AGG | - | chr3.4:99542147-99542166 | None:intergenic | 25.0% |
| ! | TTGCAAAGTAATAATATGGT+AGG | + | chr3.4:99542867-99542886 | MS.gene063148:intron | 25.0% |
| !! | AAAAATAAGATTGGTTGTGT+TGG | - | chr3.4:99542665-99542684 | None:intergenic | 25.0% |
| !! | AAAAGTTGACTAGTTGATAT+TGG | - | chr3.4:99541681-99541700 | None:intergenic | 25.0% |
| !! | ATTTTCATATCATTTAAGCC+CGG | + | chr3.4:99542327-99542346 | MS.gene063148:intron | 25.0% |
| !! | TAATGGATGAATTGTTCATT+AGG | + | chr3.4:99543845-99543864 | MS.gene063148:CDS | 25.0% |
| !! | TTTTCCAAATTGTTTGAAAG+TGG | + | chr3.4:99541957-99541976 | MS.gene063148:CDS | 25.0% |
| !! | TTTTTATATTTGTGACCAAC+CGG | - | chr3.4:99542349-99542368 | None:intergenic | 25.0% |
| !!! | ATACACAGTTTTGTTTTGAT+AGG | - | chr3.4:99543530-99543549 | None:intergenic | 25.0% |
| !!! | CACAACCAATCTTATTTTTT+TGG | + | chr3.4:99542666-99542685 | MS.gene063148:intron | 25.0% |
| !!! | TATCAAAAGCTTTTGGTAAT+TGG | - | chr3.4:99541737-99541756 | None:intergenic | 25.0% |
| !!! | TATCAACTAGTCAACTTTTT+TGG | + | chr3.4:99541682-99541701 | MS.gene063148:intron | 25.0% |
| AAAAAGTGAATGGAATTGTC+GGG | - | chr3.4:99542243-99542262 | None:intergenic | 30.0% | |
| AAGGGAATATTTACCTAACA+AGG | + | chr3.4:99542967-99542986 | MS.gene063148:CDS | 30.0% | |
| AAGGTTAGAGACAATTAGAA+CGG | + | chr3.4:99543690-99543709 | MS.gene063148:CDS | 30.0% | |
| ACTTTATGGAGTTGAGAAAT+TGG | + | chr3.4:99543888-99543907 | MS.gene063148:CDS | 30.0% | |
| AGAAAAGTGATTGAAGAAGA+AGG | + | chr3.4:99543652-99543671 | MS.gene063148:CDS | 30.0% | |
| AGAGTTTGGAGAAGAAATAA+TGG | + | chr3.4:99543828-99543847 | MS.gene063148:CDS | 30.0% | |
| AGGGAATATTTACCTAACAA+GGG | + | chr3.4:99542968-99542987 | MS.gene063148:CDS | 30.0% | |
| ATTGAGGAAAAACTGAAACA+TGG | - | chr3.4:99542082-99542101 | None:intergenic | 30.0% | |
| CATTCTCTGCATACATATAT+GGG | - | chr3.4:99541878-99541897 | None:intergenic | 30.0% | |
| CTGGACCAAAAAAATAAGAT+TGG | - | chr3.4:99542674-99542693 | None:intergenic | 30.0% | |
| CTTTGTAAATAATTAAGGCC+AGG | - | chr3.4:99543468-99543487 | None:intergenic | 30.0% | |
| GAAAAGTGATTGAAGAAGAA+GGG | + | chr3.4:99543653-99543672 | MS.gene063148:CDS | 30.0% | |
| TCGCTTGCAAAGTAATAATA+TGG | + | chr3.4:99542863-99542882 | MS.gene063148:intron | 30.0% | |
| TTGAGGAAAAACTGAAACAT+GGG | - | chr3.4:99542081-99542100 | None:intergenic | 30.0% | |
| TTTAGTCCATGTTTCTTTAG+TGG | + | chr3.4:99542186-99542205 | MS.gene063148:CDS | 30.0% | |
| TTTGACATCTCTAACACTTA+AGG | - | chr3.4:99541837-99541856 | None:intergenic | 30.0% | |
| ! | TGGACAATATCAAAAGCTTT+TGG | - | chr3.4:99541744-99541763 | None:intergenic | 30.0% |
| ! | TTTTATATTTGTGACCAACC+GGG | - | chr3.4:99542348-99542367 | None:intergenic | 30.0% |
| ! | TTTTTCCTCAATGACCTATT+TGG | + | chr3.4:99542090-99542109 | MS.gene063148:CDS | 30.0% |
| !! | CTTTAGAACCATTGAACAAA+GGG | + | chr3.4:99542949-99542968 | MS.gene063148:CDS | 30.0% |
| !!! | TCAATTTTGAAGTCAGAGTT+TGG | + | chr3.4:99543814-99543833 | MS.gene063148:CDS | 30.0% |
| AAAAGTGAATGGAATTGTCG+GGG | - | chr3.4:99542242-99542261 | None:intergenic | 35.0% | |
| AAGAGACTACAAGAAGAGAA+AGG | + | chr3.4:99542156-99542175 | MS.gene063148:CDS | 35.0% | |
| ATCAGGGAGTAACTTAAAAG+TGG | - | chr3.4:99542130-99542149 | None:intergenic | 35.0% | |
| ATTCCTCCTATAGTCTACAT+TGG | + | chr3.4:99542265-99542284 | MS.gene063148:CDS | 35.0% | |
| ATTGGTGGAGCAATCAAAAT+TGG | + | chr3.4:99543585-99543604 | MS.gene063148:CDS | 35.0% | |
| CATTTCCAAATAGGTCATTG+AGG | - | chr3.4:99542098-99542117 | None:intergenic | 35.0% | |
| CCATATATGTATGCAGAGAA+TGG | + | chr3.4:99541876-99541895 | MS.gene063148:intron | 35.0% | |
| CCATTCTCTGCATACATATA+TGG | - | chr3.4:99541879-99541898 | None:intergenic | 35.0% | |
| GGAGGAATGAAAAAAGTGAA+TGG | - | chr3.4:99542253-99542272 | None:intergenic | 35.0% | |
| TAGAGACAATTAGAACGGAT+TGG | + | chr3.4:99543695-99543714 | MS.gene063148:CDS | 35.0% | |
| TCCAAGGTAGTATACTAATC+TGG | + | chr3.4:99542291-99542310 | MS.gene063148:intron | 35.0% | |
| TCCAGATTAGTATACTACCT+TGG | - | chr3.4:99542295-99542314 | None:intergenic | 35.0% | |
| TGCAAATAACTCCACATTTC+AGG | + | chr3.4:99541627-99541646 | MS.gene063148:CDS | 35.0% | |
| TTATGGAGTTGAGAAATTGG+AGG | + | chr3.4:99543891-99543910 | MS.gene063148:CDS | 35.0% | |
| TTGGTATATGCAGAAATTGG+TGG | + | chr3.4:99543570-99543589 | MS.gene063148:intron | 35.0% | |
| ! | AAGGGTCTTTTGATGTTCAA+AGG | + | chr3.4:99543671-99543690 | MS.gene063148:CDS | 35.0% |
| ! | AGAGACAATTAGAACGGATT+GGG | + | chr3.4:99543696-99543715 | MS.gene063148:CDS | 35.0% |
| ! | ATAAATGCATGGGTTGTCAT+TGG | + | chr3.4:99543134-99543153 | MS.gene063148:CDS | 35.0% |
| ! | ATCTGCAAATATGATTGGTC+TGG | - | chr3.4:99542693-99542712 | None:intergenic | 35.0% |
| ! | ATGTTGTTTGATTACCTCTG+CGG | - | chr3.4:99543180-99543199 | None:intergenic | 35.0% |
| ! | TCAGCCACTTTCAAACAATT+TGG | - | chr3.4:99541964-99541983 | None:intergenic | 35.0% |
| !! | GCTTTAGAACCATTGAACAA+AGG | + | chr3.4:99542948-99542967 | MS.gene063148:CDS | 35.0% |
| AAGCCAATGTAGACTATAGG+AGG | - | chr3.4:99542271-99542290 | None:intergenic | 40.0% | |
| CATCTCTGCCAATAAGTGTT+AGG | - | chr3.4:99543100-99543119 | None:intergenic | 40.0% | |
| GAACAAGTCCTTCACATGAA+TGG | + | chr3.4:99541583-99541602 | MS.gene063148:CDS | 40.0% | |
| GCAATGGTCCTAACACTTAT+TGG | + | chr3.4:99543089-99543108 | MS.gene063148:CDS | 40.0% | |
| GGAAAGCCAATGTAGACTAT+AGG | - | chr3.4:99542274-99542293 | None:intergenic | 40.0% | |
| GGTGTTCCACTAAAGAAACA+TGG | - | chr3.4:99542195-99542214 | None:intergenic | 40.0% | |
| GTTTCTTTAGTGGAACACCT+GGG | + | chr3.4:99542196-99542215 | MS.gene063148:CDS | 40.0% | |
| TCATATCATTTAAGCCCGGT+TGG | + | chr3.4:99542331-99542350 | MS.gene063148:intron | 40.0% | |
| TGTTTCTTTAGTGGAACACC+TGG | + | chr3.4:99542195-99542214 | MS.gene063148:CDS | 40.0% | |
| TTCTAATCTCCTCAGACGTA+GGG | - | chr3.4:99543636-99543655 | None:intergenic | 40.0% | |
| TTTCTAATCTCCTCAGACGT+AGG | - | chr3.4:99543637-99543656 | None:intergenic | 40.0% | |
| ! | AAAGCATCTTGCAACACATC+TGG | - | chr3.4:99542933-99542952 | None:intergenic | 40.0% |
| ! | ATCAGGTCCAAATGCACTTT+TGG | + | chr3.4:99541996-99542015 | MS.gene063148:CDS | 40.0% |
| ! | GAACACCTGGGTCTTTTTAT+GGG | + | chr3.4:99542208-99542227 | MS.gene063148:CDS | 40.0% |
| ! | GACTAGTTGATATTGGAGCA+AGG | - | chr3.4:99541674-99541693 | None:intergenic | 40.0% |
| ! | GATGCAACCAAAAGTGCATT+TGG | - | chr3.4:99542006-99542025 | None:intergenic | 40.0% |
| ! | TGTTTGAAAGTGGCTGACTT+AGG | + | chr3.4:99541967-99541986 | MS.gene063148:CDS | 40.0% |
| !! | AAATATGCTTTTTGCACCGC+TGG | - | chr3.4:99543002-99543021 | None:intergenic | 40.0% |
| !! | TAGTCTACATTGGCTTTCCA+AGG | + | chr3.4:99542275-99542294 | MS.gene063148:CDS | 40.0% |
| ACTGAAACATGGGTGACTCA+TGG | - | chr3.4:99542071-99542090 | None:intergenic | 45.0% | |
| AGTCTCCCATAAAAAGACCC+AGG | - | chr3.4:99542216-99542235 | None:intergenic | 45.0% | |
| CAAGTCCTTCACATGAATGG+AGG | + | chr3.4:99541586-99541605 | MS.gene063148:CDS | 45.0% | |
| CTTCACATGAATGGAGGTGA+GGG | + | chr3.4:99541592-99541611 | MS.gene063148:CDS | 45.0% | |
| GATGAAGACACAAGAGCTGA+GGG | + | chr3.4:99543760-99543779 | MS.gene063148:CDS | 45.0% | |
| TGATGAAGACACAAGAGCTG+AGG | + | chr3.4:99543759-99543778 | MS.gene063148:CDS | 45.0% | |
| ! | GCTGACTTAGGTTGTTCATC+AGG | + | chr3.4:99541979-99541998 | MS.gene063148:CDS | 45.0% |
| ! | GGAACACCTGGGTCTTTTTA+TGG | + | chr3.4:99542207-99542226 | MS.gene063148:CDS | 45.0% |
| !! | TTTAAAAAAATTTATATAAG+CGG | - | chr3.4:99543306-99543325 | None:intergenic | 5.0% |
| !!! | TAACTTTTTTATAATATAAA+TGG | - | chr3.4:99541764-99541783 | None:intergenic | 5.0% |
| !!! | TTAATTTATTTTTTTTTGAA+AGG | - | chr3.4:99543252-99543271 | None:intergenic | 5.0% |
| ATCCTATTGCCCTACGTCTG+AGG | + | chr3.4:99543624-99543643 | MS.gene063148:CDS | 50.0% | |
| CCTCACCTCCATTCATGTGA+AGG | - | chr3.4:99541594-99541613 | None:intergenic | 50.0% | |
| CCTTCACATGAATGGAGGTG+AGG | + | chr3.4:99541591-99541610 | MS.gene063148:CDS | 50.0% | |
| TCAGACGTAGGGCAATAGGA+TGG | - | chr3.4:99543625-99543644 | None:intergenic | 50.0% | |
| TGGCATGGCACTCAATGACA+TGG | + | chr3.4:99543154-99543173 | MS.gene063148:CDS | 50.0% | |
| ! | GCAGAGAATGGTGATGCTGA+CGG | + | chr3.4:99541888-99541907 | MS.gene063148:intron | 50.0% |
| ! | TGCATGGGTTGTCATTGGCA+TGG | + | chr3.4:99543139-99543158 | MS.gene063148:CDS | 50.0% |
| !! | ATGCTTTTTGCACCGCTGGA+GGG | - | chr3.4:99542998-99543017 | None:intergenic | 50.0% |
| !! | TATGCTTTTTGCACCGCTGG+AGG | - | chr3.4:99542999-99543018 | None:intergenic | 50.0% |
| ACTCAATGACATGGCCGCAG+AGG | + | chr3.4:99543163-99543182 | MS.gene063148:CDS | 55.0% | |
| CGTTCGTCTGAACTGCTTCC+TGG | + | chr3.4:99543062-99543081 | MS.gene063148:CDS | 55.0% | |
| CTCCTCAGACGTAGGGCAAT+AGG | - | chr3.4:99543629-99543648 | None:intergenic | 55.0% | |
| GTTAGGACCATTGCACCACC+AGG | - | chr3.4:99543083-99543102 | None:intergenic | 55.0% | |
| TCGTCTGAACTGCTTCCTGG+TGG | + | chr3.4:99543065-99543084 | MS.gene063148:CDS | 55.0% | |
| ! | ACTGCTTCCTGGTGGTGCAA+TGG | + | chr3.4:99543073-99543092 | MS.gene063148:CDS | 55.0% |
| AACAAGGGCAAGCCCTCCAG+CGG | + | chr3.4:99542983-99543002 | MS.gene063148:CDS | 60.0% | |
| CTGGAGGGCTTGCCCTTGTT+AGG | - | chr3.4:99542983-99543002 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 99541574 | 99543951 | 99541574 | ID=MS.gene063148 |
| chr3.4 | mRNA | 99541574 | 99543951 | 99541574 | ID=MS.gene063148.t1;Parent=MS.gene063148 |
| chr3.4 | exon | 99541574 | 99541648 | 99541574 | ID=MS.gene063148.t1.exon1;Parent=MS.gene063148.t1 |
| chr3.4 | CDS | 99541574 | 99541648 | 99541574 | ID=cds.MS.gene063148.t1;Parent=MS.gene063148.t1 |
| chr3.4 | exon | 99541892 | 99542296 | 99541892 | ID=MS.gene063148.t1.exon2;Parent=MS.gene063148.t1 |
| chr3.4 | CDS | 99541892 | 99542296 | 99541892 | ID=cds.MS.gene063148.t1;Parent=MS.gene063148.t1 |
| chr3.4 | exon | 99542927 | 99543184 | 99542927 | ID=MS.gene063148.t1.exon3;Parent=MS.gene063148.t1 |
| chr3.4 | CDS | 99542927 | 99543184 | 99542927 | ID=cds.MS.gene063148.t1;Parent=MS.gene063148.t1 |
| chr3.4 | exon | 99543583 | 99543951 | 99543583 | ID=MS.gene063148.t1.exon4;Parent=MS.gene063148.t1 |
| chr3.4 | CDS | 99543583 | 99543951 | 99543583 | ID=cds.MS.gene063148.t1;Parent=MS.gene063148.t1 |
| Gene Sequence |
| Protein sequence |
