AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99450.t1 MTR_4g018820 97.207 358 10 0 1 358 1 358 0.0 723
MS.gene99450.t1 MTR_3g467270 81.351 370 57 2 1 359 1 369 0.0 620
MS.gene99450.t1 MTR_8g076900 73.684 361 92 1 1 358 1 361 0.0 563
MS.gene99450.t1 MTR_8g077040 70.811 370 97 2 1 359 1 370 0.0 548
MS.gene99450.t1 MTR_5g020940 64.324 370 120 3 1 358 11 380 0.0 508
MS.gene99450.t1 MTR_6g056070 68.611 360 109 2 1 359 1 357 2.28e-179 501
MS.gene99450.t1 MTR_8g020690 62.674 359 133 1 1 358 1 359 1.83e-170 479
MS.gene99450.t1 MTR_8g020700 61.560 359 134 2 1 358 1 356 8.54e-163 459
MS.gene99450.t1 MTR_8g076900 75.918 245 59 0 1 245 1 245 7.04e-139 395
MS.gene99450.t1 MTR_8g020700 64.706 255 89 1 1 254 1 255 1.51e-118 343
MS.gene99450.t1 MTR_3g117060 45.455 374 182 4 1 358 1 368 4.25e-112 331
MS.gene99450.t1 MTR_8g077030 54.728 349 76 8 1 347 1 269 7.71e-109 328
MS.gene99450.t1 MTR_3g467560 42.507 367 197 5 1 357 25 387 5.76e-101 303
MS.gene99450.t1 MTR_1g022455 43.651 378 193 7 1 358 1 378 4.89e-100 301
MS.gene99450.t1 MTR_1g022465 43.767 377 193 6 1 358 1 377 7.06e-99 298
MS.gene99450.t1 MTR_5g046110 38.904 365 210 3 1 357 61 420 1.56e-95 291
MS.gene99450.t1 MTR_7g084620 41.444 374 195 8 1 359 1 365 2.88e-95 288
MS.gene99450.t1 MTR_7g084350 39.024 369 205 6 5 359 3 365 1.78e-88 271
MS.gene99450.t1 MTR_7g084330 36.857 350 182 10 25 359 8 333 1.15e-66 214
MS.gene99450.t1 MTR_3g054330 36.901 355 197 12 1 335 10 357 1.07e-57 192
MS.gene99450.t1 MTR_5g093900 34.031 382 221 12 1 356 13 389 4.94e-55 185
MS.gene99450.t1 MTR_3g052700 34.807 362 209 11 1 340 13 369 2.89e-54 183
MS.gene99450.t1 MTR_1g108660 36.420 324 172 10 26 335 9 312 4.59e-52 176
MS.gene99450.t1 MTR_6g015620 37.097 124 66 2 245 358 16 137 3.26e-20 85.9
MS.gene99450.t1 MTR_7g084340 31.250 128 81 2 237 357 2 129 1.79e-16 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99450.t1 AT1G19640 44.961 387 183 6 1 357 1 387 4.49e-100 301
MS.gene99450.t1 AT3G11480 41.737 357 203 3 4 358 26 379 2.24e-96 291
MS.gene99450.t1 AT4G36470 40.771 363 198 5 9 358 12 370 9.12e-94 285
MS.gene99450.t1 AT5G66430 41.111 360 196 5 9 358 1 354 3.46e-93 282
MS.gene99450.t1 AT5G04370 42.297 357 196 5 7 358 39 390 5.07e-91 278
MS.gene99450.t1 AT5G04370 42.297 357 196 5 7 358 44 395 1.35e-90 277
MS.gene99450.t1 AT5G04370 42.297 357 196 5 7 358 58 409 1.39e-90 278
MS.gene99450.t1 AT5G04380 40.278 360 203 4 6 358 35 389 1.50e-90 277
MS.gene99450.t1 AT5G38020 41.892 370 194 6 6 358 3 368 4.45e-89 272
MS.gene99450.t1 AT3G21950 41.734 369 196 5 6 358 3 368 2.36e-87 268
MS.gene99450.t1 AT2G14060 40.710 366 200 6 6 358 11 372 2.98e-86 265
MS.gene99450.t1 AT5G04380 39.722 360 198 5 6 358 31 378 6.12e-86 265
MS.gene99450.t1 AT5G04380 41.325 317 175 3 48 358 7 318 3.29e-84 258
MS.gene99450.t1 AT5G38020 42.508 327 168 5 48 358 7 329 3.26e-82 253
MS.gene99450.t1 AT3G21950 38.653 401 195 6 6 358 3 400 1.84e-81 254
MS.gene99450.t1 AT2G14060 46.053 228 119 2 6 232 11 235 8.26e-65 206
MS.gene99450.t1 AT1G68040 36.705 346 190 10 7 338 11 341 6.63e-61 200
MS.gene99450.t1 AT1G15125 35.015 337 190 10 9 333 1 320 7.65e-56 186
MS.gene99450.t1 AT5G55250 34.957 349 207 10 1 332 13 358 1.20e-54 184
MS.gene99450.t1 AT5G37970 35.294 374 205 13 5 358 6 362 2.90e-53 180
MS.gene99450.t1 AT5G38100 35.933 359 186 13 3 340 4 339 4.21e-52 177
MS.gene99450.t1 AT5G37990 34.584 373 209 11 5 358 6 362 5.78e-52 176
MS.gene99450.t1 AT5G38780 33.521 355 202 10 3 340 4 341 1.23e-50 173
MS.gene99450.t1 AT5G55250 36.066 305 177 9 43 332 19 320 1.96e-50 172
MS.gene99450.t1 AT5G38100 38.284 303 156 10 51 340 32 316 1.85e-49 169
MS.gene99450.t1 AT4G26420 32.578 353 203 12 5 333 9 350 2.92e-48 167
MS.gene99450.t1 AT5G38780 34.653 303 168 8 51 340 7 292 1.72e-47 163
MS.gene99450.t1 AT5G56300 31.161 353 209 11 5 334 20 361 1.85e-47 165
MS.gene99450.t1 AT3G44840 33.433 335 195 9 9 334 7 322 1.31e-42 151
MS.gene99450.t1 AT1G66700 30.564 337 197 11 9 330 9 323 3.12e-38 140
MS.gene99450.t1 AT1G66720 30.376 372 224 11 1 358 1 351 4.64e-38 139
MS.gene99450.t1 AT3G44870 36.140 285 156 9 9 284 7 274 2.08e-37 138
MS.gene99450.t1 AT4G26420 35.547 256 139 9 5 243 9 255 3.72e-37 135
MS.gene99450.t1 AT3G44860 34.948 289 157 9 9 284 7 277 6.68e-37 136
MS.gene99450.t1 AT4G26420 35.317 252 137 9 9 243 1 243 9.85e-36 131
MS.gene99450.t1 AT1G15125 34.831 267 143 10 9 263 1 248 2.89e-35 129
MS.gene99450.t1 AT5G38100 36.641 262 127 11 3 243 4 247 2.93e-34 127
MS.gene99450.t1 AT5G38100 36.641 262 127 11 3 243 4 247 2.98e-34 127
MS.gene99450.t1 AT1G66690 30.321 343 206 10 1 330 1 323 4.27e-34 129
MS.gene99450.t1 AT1G66700 32.819 259 144 9 1 245 1 243 2.24e-28 111

Find 77 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGATTTGGGTTGCTCTTT+TGG 0.145254 4.2:+83523848 MS.gene99450:CDS
TGCTACTTCTCTGGTGTTCC+TGG 0.206346 4.2:+83524064 MS.gene99450:CDS
ATTGGAATGCTCTTGATAAT+TGG 0.222028 4.2:+83524794 MS.gene99450:CDS
TAGAGGTTTCTGAAGTAAAT+TGG 0.228818 4.2:+83524776 MS.gene99450:CDS
TCTGCAATTGCCAAGCTTCT+AGG 0.248424 4.2:-83523830 None:intergenic
GGCTATGGCTCTTAATGATA+TGG 0.252043 4.2:+83524487 MS.gene99450:CDS
AAGCTTGGCAATTGCAGATT+TGG 0.258208 4.2:+83523835 MS.gene99450:CDS
TGGAAAGATCCTACCATAAA+AGG 0.266724 4.2:-83524089 None:intergenic
ATGGTTTCCTAGTCATCATT+AGG 0.273813 4.2:-83524984 None:intergenic
CAGTCTTCAATGGCTATCTA+AGG 0.306294 4.2:+83524144 MS.gene99450:CDS
GATGGAGATGGATAATAGTT+AGG 0.322863 4.2:-83524703 None:intergenic
GGTGAACTTAGTCTTCTCTT+TGG 0.329569 4.2:-83524953 None:intergenic
GCAAGGAGTCTGGTTACATT+TGG 0.334076 4.2:+83524456 MS.gene99450:CDS
TTCTTCAACACTTCCATTCA+TGG 0.347205 4.2:-83523241 None:intergenic
GGCTGTGGCTGAACCATTGC+TGG 0.354555 4.2:+83524858 MS.gene99450:CDS
TAATGAAATGGAAACTGAAA+TGG 0.362246 4.2:+83524036 MS.gene99450:CDS
TAACCAGACTCCTTGCTTGA+TGG 0.366400 4.2:-83524449 None:intergenic
TTGGAATGCTCTTGATAATT+GGG 0.367672 4.2:+83524795 MS.gene99450:CDS
AGCTTGGCAATTGCAGATTT+GGG 0.370897 4.2:+83523836 MS.gene99450:CDS
CAAGGAGTCTGGTTACATTT+GGG 0.398855 4.2:+83524457 MS.gene99450:CDS
TGGTTTCCTAGTCATCATTA+GGG 0.408825 4.2:-83524983 None:intergenic
ACCACCTTCAACTAGTTCTT+CGG 0.413226 4.2:-83524387 None:intergenic
TCGAGGGGCTTGTGCTCGTC+AGG 0.418348 4.2:-83524301 None:intergenic
TGCAAACAACTCCTTACTTC+AGG 0.420015 4.2:+83523272 MS.gene99450:CDS
GAGATCACCAAGAAAGTGTT+TGG 0.420623 4.2:-83523872 None:intergenic
GTTGGAGTATAGACTGGTTA+TGG 0.439646 4.2:-83523801 None:intergenic
ACAATGTGGCACAATGCATA+AGG 0.440440 4.2:+83524836 MS.gene99450:CDS
CATTTGGGAGCTCATGGCTA+TGG 0.450239 4.2:+83524472 MS.gene99450:CDS
TTTGTATAGATTCCTGAGGT+TGG 0.452696 4.2:+83524254 MS.gene99450:intron
ATGGGCCCATGCTACTTCTC+TGG 0.453799 4.2:+83524055 MS.gene99450:CDS
AATTGGGATCAATTTAATGA+TGG 0.454125 4.2:+83524811 MS.gene99450:CDS
TAGCCATTGAAGACTGTAAG+AGG 0.461670 4.2:-83524137 None:intergenic
TGAAACTATTGAGTTTCTCT+TGG 0.465227 4.2:-83524675 None:intergenic
ATCTCCATCTGAGGTGAAGT+TGG 0.465780 4.2:+83524717 MS.gene99450:CDS
ACGTCGTTGAAATCATTTCC+CGG 0.465836 4.2:-83523977 None:intergenic
GGTGATCTCTGAAATTATCA+AGG 0.471395 4.2:+83523886 MS.gene99450:CDS
AAGCCCCTCGAATATCTTCA+AGG 0.472433 4.2:+83524313 MS.gene99450:CDS
AGTCATCATTAGGGTAAGAT+TGG 0.479471 4.2:-83524974 None:intergenic
TTGTTGTTGTCTCCAACCTC+AGG 0.487963 4.2:-83524266 None:intergenic
CCATTGCTGGTTAGTCACTT+TGG 0.497066 4.2:+83524871 MS.gene99450:CDS
GATCCATCAAGCAAGGAGTC+TGG 0.498680 4.2:+83524446 MS.gene99450:CDS
ATGGAGATGGATAATAGTTA+GGG 0.500415 4.2:-83524702 None:intergenic
ATTCCTCTTACAGTCTTCAA+TGG 0.509521 4.2:+83524134 MS.gene99450:CDS
CAAACTATATACCTGAAGTA+AGG 0.512115 4.2:-83523283 None:intergenic
ACTTCCAACTTCACCTCAGA+TGG 0.513220 4.2:-83524721 None:intergenic
AATGAAATGGAAACTGAAAT+GGG 0.514173 4.2:+83524037 MS.gene99450:CDS
TGGTTACATTTGGGAGCTCA+TGG 0.518558 4.2:+83524466 MS.gene99450:CDS
CTGGTTAGTCACTTTGGTGA+AGG 0.524926 4.2:+83524877 MS.gene99450:CDS
ACTGATGGAGAATGATCCTT+CGG 0.525237 4.2:-83524749 None:intergenic
AAGTGTGTTGGAGTATAGAC+TGG 0.526629 4.2:-83523807 None:intergenic
CAATGTGGCACAATGCATAA+GGG 0.527168 4.2:+83524837 MS.gene99450:CDS
ATCCTACCATAAAAGGATCC+AGG 0.532082 4.2:-83524082 None:intergenic
TTATTACAAGCAATTTCAAA+CGG 0.544915 4.2:+83524337 MS.gene99450:CDS
CAACACACTTCCTAGAAGCT+TGG 0.551062 4.2:+83523820 MS.gene99450:CDS
TCTTCAACACTTCCATTCAT+GGG 0.552017 4.2:-83523240 None:intergenic
AGAAACCTCTAGTCGACTGA+TGG 0.559555 4.2:-83524764 None:intergenic
ACTCAGGTACTACCCATGAA+TGG 0.562098 4.2:+83523228 MS.gene99450:CDS
CGTGCCGAAGAACTAGTTGA+AGG 0.567726 4.2:+83524383 MS.gene99450:CDS
ATTCTCCATCAGTCGACTAG+AGG 0.583884 4.2:+83524759 MS.gene99450:CDS
AACTTCACCTCAGATGGAGA+TGG 0.586655 4.2:-83524715 None:intergenic
GGAACACCAGAGAAGTAGCA+TGG 0.591913 4.2:-83524061 None:intergenic
CAAGCTTCTAGGAAGTGTGT+TGG 0.606427 4.2:-83523819 None:intergenic
AAACAGTGATCCATCAAGCA+AGG 0.606931 4.2:+83524439 MS.gene99450:CDS
TAAGCCTTGAAGATATTCGA+GGG 0.607031 4.2:-83524317 None:intergenic
CTATTATCCATCTCCATCTG+AGG 0.607939 4.2:+83524708 MS.gene99450:CDS
ATAAGCCTTGAAGATATTCG+AGG 0.609948 4.2:-83524318 None:intergenic
ATCTTACCCTAATGATGACT+AGG 0.614467 4.2:+83524977 MS.gene99450:CDS
CCAAAGTGACTAACCAGCAA+TGG 0.615902 4.2:-83524871 None:intergenic
GAACACCAGAGAAGTAGCAT+GGG 0.633213 4.2:-83524060 None:intergenic
AAGTTGGAAGTTCTCACCGA+AGG 0.633680 4.2:+83524733 MS.gene99450:CDS
TCTATTTGTATAGATTCCTG+AGG 0.634224 4.2:+83524250 MS.gene99450:intron
GGCACAATGCATAAGGGCTG+TGG 0.641920 4.2:+83524843 MS.gene99450:CDS
GCCGAAGAACTAGTTGAAGG+TGG 0.672957 4.2:+83524386 MS.gene99450:CDS
AAGCCTTGAAGATATTCGAG+GGG 0.677862 4.2:-83524316 None:intergenic
TGAGGTTGGAGACAACAACA+AGG 0.685230 4.2:+83524268 MS.gene99450:CDS
ATTTAATGATGGATACAATG+TGG 0.700017 4.2:+83524822 MS.gene99450:CDS
GAGGTTGGAGACAACAACAA+GGG 0.741052 4.2:+83524269 MS.gene99450:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAGAAGAATTTATTGT+TGG - chr4.2:83523526-83523545 None:intergenic 15.0%
!! AATGAAATAGAGAATAAAAA+TGG - chr4.2:83523563-83523582 None:intergenic 15.0%
!! ATAGAGAAAAATTTATATTG+TGG - chr4.2:83524224-83524243 None:intergenic 15.0%
!! GATATGATGATAATATATAT+AGG - chr4.2:83523322-83523341 None:intergenic 15.0%
!! GTTCATATTAATTTAGAAAA+TGG + chr4.2:83524541-83524560 MS.gene99450:intron 15.0%
!!! ATTTTTTTTCTTCAAAGTTT+TGG - chr4.2:83524610-83524629 None:intergenic 15.0%
!! TTATTACAAGCAATTTCAAA+CGG + chr4.2:83524337-83524356 MS.gene99450:CDS 20.0%
!! TTCAAAAAGACTTTATATTC+AGG - chr4.2:83523950-83523969 None:intergenic 20.0%
!! TTGATTGAAATTGTCTAATA+TGG + chr4.2:83523493-83523512 MS.gene99450:intron 20.0%
!!! AATCGCTATAAGAAAATTTT+AGG + chr4.2:83524919-83524938 MS.gene99450:CDS 20.0%
! AAATTTGAATATACAGCGAA+AGG + chr4.2:83523745-83523764 MS.gene99450:intron 25.0%
! AATGAAATGGAAACTGAAAT+GGG + chr4.2:83524037-83524056 MS.gene99450:CDS 25.0%
! AATTGGGATCAATTTAATGA+TGG + chr4.2:83524811-83524830 MS.gene99450:CDS 25.0%
! AGAAAAACTACGTAATGAAA+TGG + chr4.2:83524024-83524043 MS.gene99450:CDS 25.0%
! ATAGAACACAATAAATACGT+GGG + chr4.2:83524566-83524585 MS.gene99450:intron 25.0%
! GAAATTGTCTAATATGGATT+CGG + chr4.2:83523499-83523518 MS.gene99450:intron 25.0%
! TAATGAAATGGAAACTGAAA+TGG + chr4.2:83524036-83524055 MS.gene99450:CDS 25.0%
!! ACAAAATGCAAACTTTTATC+TGG - chr4.2:83524112-83524131 None:intergenic 25.0%
!! ATTACGTAGTTTTTCTTTGA+AGG - chr4.2:83524020-83524039 None:intergenic 25.0%
!! CACATGATTCTAACATTTTT+AGG + chr4.2:83524410-83524429 MS.gene99450:CDS 25.0%
!!! ATTTAATGATGGATACAATG+TGG + chr4.2:83524822-83524841 MS.gene99450:CDS 25.0%
!!! TATCGAATTAAAGAAGTATC+AGG + chr4.2:83523388-83523407 MS.gene99450:intron 25.0%
AAACTTGAGTGTGTCTAATA+TGG - chr4.2:83523637-83523656 None:intergenic 30.0%
ATGGAGATGGATAATAGTTA+GGG - chr4.2:83524705-83524724 None:intergenic 30.0%
ATGTGAAAAATAGCTTGAGA+CGG + chr4.2:83523696-83523715 MS.gene99450:intron 30.0%
CAAACTATATACCTGAAGTA+AGG - chr4.2:83523286-83523305 None:intergenic 30.0%
CATAGAACACAATAAATACG+TGG + chr4.2:83524565-83524584 MS.gene99450:intron 30.0%
GTGGCGAAACATTTATTAAA+TGG - chr4.2:83524205-83524224 None:intergenic 30.0%
TCTATTTGTATAGATTCCTG+AGG + chr4.2:83524250-83524269 MS.gene99450:intron 30.0%
TGAAACTATTGAGTTTCTCT+TGG - chr4.2:83524678-83524697 None:intergenic 30.0%
! ATTGGAATGCTCTTGATAAT+TGG + chr4.2:83524794-83524813 MS.gene99450:CDS 30.0%
! TAATTTAGACCTTTCTAGAG+AGG + chr4.2:83523610-83523629 MS.gene99450:intron 30.0%
! TCTCTAGAAAGGTCTAAATT+AGG - chr4.2:83523611-83523630 None:intergenic 30.0%
! TTGGAATGCTCTTGATAATT+GGG + chr4.2:83524795-83524814 MS.gene99450:CDS 30.0%
!! TAGAGGTTTCTGAAGTAAAT+TGG + chr4.2:83524776-83524795 MS.gene99450:CDS 30.0%
!! TCTTAATGATATGGTTTTGC+AGG + chr4.2:83524496-83524515 MS.gene99450:CDS 30.0%
!!! GGTTTGTTATCTTAGTCTTT+TGG + chr4.2:83524165-83524184 MS.gene99450:intron 30.0%
!!! TTTTTCTTTGAAGGTGTCAA+GGG - chr4.2:83524011-83524030 None:intergenic 30.0%
AGTCATCATTAGGGTAAGAT+TGG - chr4.2:83524977-83524996 None:intergenic 35.0%
ATAAGCCTTGAAGATATTCG+AGG - chr4.2:83524321-83524340 None:intergenic 35.0%
ATAGCTTGAGACGGATATTA+TGG + chr4.2:83523705-83523724 MS.gene99450:intron 35.0%
ATCTTACCCTAATGATGACT+AGG + chr4.2:83524977-83524996 MS.gene99450:CDS 35.0%
ATGCATATTATTATGCCGCA+GGG + chr4.2:83524643-83524662 MS.gene99450:intron 35.0%
ATGGTTTCCTAGTCATCATT+AGG - chr4.2:83524987-83525006 None:intergenic 35.0%
ATTCCTCTTACAGTCTTCAA+TGG + chr4.2:83524134-83524153 MS.gene99450:CDS 35.0%
CTAGAAAGGTCTAAATTAGG+TGG - chr4.2:83523608-83523627 None:intergenic 35.0%
GATGGAGATGGATAATAGTT+AGG - chr4.2:83524706-83524725 None:intergenic 35.0%
TAAGCCTTGAAGATATTCGA+GGG - chr4.2:83524320-83524339 None:intergenic 35.0%
TATGCATATTATTATGCCGC+AGG + chr4.2:83524642-83524661 MS.gene99450:intron 35.0%
TCTTCAACACTTCCATTCAT+GGG - chr4.2:83523243-83523262 None:intergenic 35.0%
TGGAAAGATCCTACCATAAA+AGG - chr4.2:83524092-83524111 None:intergenic 35.0%
TGGTTTCCTAGTCATCATTA+GGG - chr4.2:83524986-83525005 None:intergenic 35.0%
TTCTTCAACACTTCCATTCA+TGG - chr4.2:83523244-83523263 None:intergenic 35.0%
TTTGTATAGATTCCTGAGGT+TGG + chr4.2:83524254-83524273 MS.gene99450:intron 35.0%
! AGTCTTTTTGAACGATCTAC+CGG + chr4.2:83523958-83523977 MS.gene99450:CDS 35.0%
! TAATATGGACCTCTCTAGAA+AGG - chr4.2:83523622-83523641 None:intergenic 35.0%
! TTTTGGACCAAACACTTTCT+TGG + chr4.2:83523865-83523884 MS.gene99450:CDS 35.0%
!! GGTGATCTCTGAAATTATCA+AGG + chr4.2:83523886-83523905 MS.gene99450:CDS 35.0%
!!! GTTTTTCTTTGAAGGTGTCA+AGG - chr4.2:83524012-83524031 None:intergenic 35.0%
AAACAGTGATCCATCAAGCA+AGG + chr4.2:83524439-83524458 MS.gene99450:CDS 40.0%
AAGCCTTGAAGATATTCGAG+GGG - chr4.2:83524319-83524338 None:intergenic 40.0%
AAGCTTGGCAATTGCAGATT+TGG + chr4.2:83523835-83523854 MS.gene99450:CDS 40.0%
AAGTGTGTTGGAGTATAGAC+TGG - chr4.2:83523810-83523829 None:intergenic 40.0%
ACAATGTGGCACAATGCATA+AGG + chr4.2:83524836-83524855 MS.gene99450:CDS 40.0%
ACGTCGTTGAAATCATTTCC+CGG - chr4.2:83523980-83523999 None:intergenic 40.0%
ACTGATGGAGAATGATCCTT+CGG - chr4.2:83524752-83524771 None:intergenic 40.0%
AGCTTGGCAATTGCAGATTT+GGG + chr4.2:83523836-83523855 MS.gene99450:CDS 40.0%
ATCCTACCATAAAAGGATCC+AGG - chr4.2:83524085-83524104 None:intergenic 40.0%
CAAGGAGTCTGGTTACATTT+GGG + chr4.2:83524457-83524476 MS.gene99450:CDS 40.0%
CAATGTGGCACAATGCATAA+GGG + chr4.2:83524837-83524856 MS.gene99450:CDS 40.0%
CAGTCTTCAATGGCTATCTA+AGG + chr4.2:83524144-83524163 MS.gene99450:CDS 40.0%
CTATTATCCATCTCCATCTG+AGG + chr4.2:83524708-83524727 MS.gene99450:CDS 40.0%
GGCTATGGCTCTTAATGATA+TGG + chr4.2:83524487-83524506 MS.gene99450:CDS 40.0%
TAGCCATTGAAGACTGTAAG+AGG - chr4.2:83524140-83524159 None:intergenic 40.0%
TGCAAACAACTCCTTACTTC+AGG + chr4.2:83523272-83523291 MS.gene99450:CDS 40.0%
! ACCACCTTCAACTAGTTCTT+CGG - chr4.2:83524390-83524409 None:intergenic 40.0%
! GGTGAACTTAGTCTTCTCTT+TGG - chr4.2:83524956-83524975 None:intergenic 40.0%
! GTCTTTTTGAACGATCTACC+GGG + chr4.2:83523959-83523978 MS.gene99450:CDS 40.0%
! GTTGGAGTATAGACTGGTTA+TGG - chr4.2:83523804-83523823 None:intergenic 40.0%
! TTCCTGGATCCTTTTATGGT+AGG + chr4.2:83524080-83524099 MS.gene99450:CDS 40.0%
!! GAGATCACCAAGAAAGTGTT+TGG - chr4.2:83523875-83523894 None:intergenic 40.0%
AACTTCACCTCAGATGGAGA+TGG - chr4.2:83524718-83524737 None:intergenic 45.0%
AAGCCCCTCGAATATCTTCA+AGG + chr4.2:83524313-83524332 MS.gene99450:CDS 45.0%
AAGTTGGAAGTTCTCACCGA+AGG + chr4.2:83524733-83524752 MS.gene99450:CDS 45.0%
ACTCAGGTACTACCCATGAA+TGG + chr4.2:83523228-83523247 MS.gene99450:CDS 45.0%
ACTTCCAACTTCACCTCAGA+TGG - chr4.2:83524724-83524743 None:intergenic 45.0%
AGAAACCTCTAGTCGACTGA+TGG - chr4.2:83524767-83524786 None:intergenic 45.0%
ATTCTCCATCAGTCGACTAG+AGG + chr4.2:83524759-83524778 MS.gene99450:CDS 45.0%
CAACACACTTCCTAGAAGCT+TGG + chr4.2:83523820-83523839 MS.gene99450:CDS 45.0%
CCAAAGTGACTAACCAGCAA+TGG - chr4.2:83524874-83524893 None:intergenic 45.0%
GAACACCAGAGAAGTAGCAT+GGG - chr4.2:83524063-83524082 None:intergenic 45.0%
GAGGTTGGAGACAACAACAA+GGG + chr4.2:83524269-83524288 MS.gene99450:CDS 45.0%
GCAAGGAGTCTGGTTACATT+TGG + chr4.2:83524456-83524475 MS.gene99450:CDS 45.0%
TAACCAGACTCCTTGCTTGA+TGG - chr4.2:83524452-83524471 None:intergenic 45.0%
TCTGCAATTGCCAAGCTTCT+AGG - chr4.2:83523833-83523852 None:intergenic 45.0%
TCTTGGTCAATGATTCCCTG+CGG - chr4.2:83524661-83524680 None:intergenic 45.0%
TGAGGTTGGAGACAACAACA+AGG + chr4.2:83524268-83524287 MS.gene99450:CDS 45.0%
TGGTTACATTTGGGAGCTCA+TGG + chr4.2:83524466-83524485 MS.gene99450:CDS 45.0%
! ATCTCCATCTGAGGTGAAGT+TGG + chr4.2:83524717-83524736 MS.gene99450:CDS 45.0%
! CAAGCTTCTAGGAAGTGTGT+TGG - chr4.2:83523822-83523841 None:intergenic 45.0%
! CCATTGCTGGTTAGTCACTT+TGG + chr4.2:83524871-83524890 MS.gene99450:CDS 45.0%
! CTGGTTAGTCACTTTGGTGA+AGG + chr4.2:83524877-83524896 MS.gene99450:CDS 45.0%
! GGTGTTCCTGGATCCTTTTA+TGG + chr4.2:83524076-83524095 MS.gene99450:CDS 45.0%
! TTGTTGTTGTCTCCAACCTC+AGG - chr4.2:83524269-83524288 None:intergenic 45.0%
!!! GCAGATTTGGGTTGCTCTTT+TGG + chr4.2:83523848-83523867 MS.gene99450:CDS 45.0%
!!! ATTTCATTTTTTTTTTTAAA+TGG + chr4.2:83523575-83523594 MS.gene99450:intron 5.0%
CATTTGGGAGCTCATGGCTA+TGG + chr4.2:83524472-83524491 MS.gene99450:CDS 50.0%
CGTGCCGAAGAACTAGTTGA+AGG + chr4.2:83524383-83524402 MS.gene99450:CDS 50.0%
GATCCATCAAGCAAGGAGTC+TGG + chr4.2:83524446-83524465 MS.gene99450:CDS 50.0%
GCCGAAGAACTAGTTGAAGG+TGG + chr4.2:83524386-83524405 MS.gene99450:CDS 50.0%
GGAACACCAGAGAAGTAGCA+TGG - chr4.2:83524064-83524083 None:intergenic 50.0%
! TGCTACTTCTCTGGTGTTCC+TGG + chr4.2:83524064-83524083 MS.gene99450:CDS 50.0%
GGCACAATGCATAAGGGCTG+TGG + chr4.2:83524843-83524862 MS.gene99450:CDS 55.0%
! ATGGGCCCATGCTACTTCTC+TGG + chr4.2:83524055-83524074 MS.gene99450:CDS 55.0%
GGCTGTGGCTGAACCATTGC+TGG + chr4.2:83524858-83524877 MS.gene99450:CDS 60.0%
TCGAGGGGCTTGTGCTCGTC+AGG - chr4.2:83524304-83524323 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 83523219 83525008 83523219 ID=MS.gene99450
chr4.2 mRNA 83523219 83525008 83523219 ID=MS.gene99450.t1;Parent=MS.gene99450
chr4.2 exon 83523219 83523293 83523219 ID=MS.gene99450.t1.exon1;Parent=MS.gene99450.t1
chr4.2 CDS 83523219 83523293 83523219 ID=cds.MS.gene99450.t1;Parent=MS.gene99450.t1
chr4.2 exon 83523761 83524165 83523761 ID=MS.gene99450.t1.exon2;Parent=MS.gene99450.t1
chr4.2 CDS 83523761 83524165 83523761 ID=cds.MS.gene99450.t1;Parent=MS.gene99450.t1
chr4.2 exon 83524263 83524517 83524263 ID=MS.gene99450.t1.exon3;Parent=MS.gene99450.t1
chr4.2 CDS 83524263 83524517 83524263 ID=cds.MS.gene99450.t1;Parent=MS.gene99450.t1
chr4.2 exon 83524664 83525008 83524664 ID=MS.gene99450.t1.exon4;Parent=MS.gene99450.t1
chr4.2 CDS 83524664 83525008 83524664 ID=cds.MS.gene99450.t1;Parent=MS.gene99450.t1
Gene Sequence

>MS.gene99450

ATGGAAGCAACTCAGGTACTACCCATGAATGGAAGTGTTGAAGAAGCAAGCTATGCAAACAACTCCTTACTTCAGGTATATAGTTTGTCTATTTATCTATCCTATATATATTATCATCATATCTTCATCTAAAGTCTATATATATTATCATCATATCTTCATCTAAAGTTATCGAATTAAAGAAGTATCAGGTAATGCTAATTTTTAAATTTATGAAAATGTGTGAATTTAATCTACATATCAATAAGAACTTTTTGAGATGAAATTCTATACGTTGATTGAAATTGTCTAATATGGATTCGGTCCAACAATAAATTCTTCTATTTTAAATAAAGTTAATACCATTTTTATTCTCTATTTCATTTTTTTTTTTAAATGGTTAATATCCACCTAATTTAGACCTTTCTAGAGAGGTCCATATTAGACACACTCAAGTTTATTATACAAAAATATAAATAATATTATCTTCATATTTAAATGTGAAAAATAGCTTGAGACGGATATTATGGTCTGAATATTAAGCATCAAATTTGAATATACAGCGAAAGGTGATTTCTTTGACAAAATCTTTGAGAGACGAAGCCATAACCAGTCTATACTCCAACACACTTCCTAGAAGCTTGGCAATTGCAGATTTGGGTTGCTCTTTTGGACCAAACACTTTCTTGGTGATCTCTGAAATTATCAAGGTTGTTGAAAAGCTTTGTCAAGAACTTAATCATAAATCTCCTGAATATAAAGTCTTTTTGAACGATCTACCGGGAAATGATTTCAACGACGTATTTATGTCCCTTGACACCTTCAAAGAAAAACTACGTAATGAAATGGAAACTGAAATGGGCCCATGCTACTTCTCTGGTGTTCCTGGATCCTTTTATGGTAGGATCTTTCCAGATAAAAGTTTGCATTTTGTGCATTCCTCTTACAGTCTTCAATGGCTATCTAAGGTTTGTTATCTTAGTCTTTTGGTAATCTATTAACATCCATTTAATAAATGTTTCGCCACAATATAAATTTTTCTCTATATTTAATCTATTTGTATAGATTCCTGAGGTTGGAGACAACAACAAGGGTAACATTTACCTGACGAGCACAAGCCCCTCGAATATCTTCAAGGCTTATTACAAGCAATTTCAAACGGATTTCTCTTTTTTTCTCAAGTGTCGTGCCGAAGAACTAGTTGAAGGTGGTCACATGATTCTAACATTTTTAGGACGAAAAAACAGTGATCCATCAAGCAAGGAGTCTGGTTACATTTGGGAGCTCATGGCTATGGCTCTTAATGATATGGTTTTGCAGGTAGACTATGATAATTTTTTCTTGTTCATATTAATTTAGAAAATGGTCATAGAACACAATAAATACGTGGGTGAATATGAGAAAAAACTCCAAAACTTTGAAGAAAAAAAATTAACTATATTAATATGCATATTATTATGCCGCAGGGAATCATTGACCAAGAGAAACTCAATAGTTTCAACATCCCTAACTATTATCCATCTCCATCTGAGGTGAAGTTGGAAGTTCTCACCGAAGGATCATTCTCCATCAGTCGACTAGAGGTTTCTGAAGTAAATTGGAATGCTCTTGATAATTGGGATCAATTTAATGATGGATACAATGTGGCACAATGCATAAGGGCTGTGGCTGAACCATTGCTGGTTAGTCACTTTGGTGAAGGTGTCATTAAAGAGATTTTTAATCGCTATAAGAAAATTTTAGGCGATCGCATGTCCAAAGAGAAGACTAAGTTCACCAATCTTACCCTAATGATGACTAGGAAACCATGA
Protein sequence

>MS.gene99450.t1

MEATQVLPMNGSVEEASYANNSLLQRKVISLTKSLRDEAITSLYSNTLPRSLAIADLGCSFGPNTFLVISEIIKVVEKLCQELNHKSPEYKVFLNDLPGNDFNDVFMSLDTFKEKLRNEMETEMGPCYFSGVPGSFYGRIFPDKSLHFVHSSYSLQWLSKIPEVGDNNKGNIYLTSTSPSNIFKAYYKQFQTDFSFFLKCRAEELVEGGHMILTFLGRKNSDPSSKESGYIWELMAMALNDMVLQGIIDQEKLNSFNIPNYYPSPSEVKLEVLTEGSFSISRLEVSEVNWNALDNWDQFNDGYNVAQCIRAVAEPLLVSHFGEGVIKEIFNRYKKILGDRMSKEKTKFTNLTLMMTRKP