AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene035837


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035837.t1 MTR_5g064580 99.615 260 1 0 1 260 1 260 0.0 532
MS.gene035837.t1 MTR_3g099380 90.385 260 25 0 1 260 1 260 1.18e-176 486
MS.gene035837.t1 MTR_3g099380 89.655 203 21 0 1 203 1 203 2.48e-135 380
MS.gene035837.t1 MTR_3g100620 75.949 237 56 1 8 243 11 247 2.51e-135 382
MS.gene035837.t1 MTR_2g091225 72.016 243 66 1 8 250 7 247 9.18e-129 365
MS.gene035837.t1 MTR_8g086270 71.605 243 67 1 8 250 7 247 4.64e-126 358
MS.gene035837.t1 MTR_2g015980 69.020 255 72 3 6 256 3 254 5.52e-126 358
MS.gene035837.t1 MTR_5g044160 70.325 246 71 1 3 248 2 245 2.16e-124 354
MS.gene035837.t1 MTR_4g083060 68.898 254 72 2 8 256 7 258 7.59e-124 354
MS.gene035837.t1 MTR_2g076960 66.154 260 85 2 1 259 1 258 1.07e-123 353
MS.gene035837.t1 MTR_2g091225 70.222 225 65 1 26 250 2 224 5.56e-116 332
MS.gene035837.t1 MTR_0137s0020 58.403 238 93 4 8 243 11 244 1.69e-91 270
MS.gene035837.t1 MTR_3g014060 57.983 238 94 4 8 243 11 244 1.11e-90 268
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035837.t1 AT1G78300 90.157 254 24 1 8 260 5 258 1.70e-169 468
MS.gene035837.t1 AT1G35160 85.985 264 33 2 1 260 1 264 1.79e-167 464
MS.gene035837.t1 AT4G09000 88.933 253 26 1 1 251 1 253 2.43e-166 461
MS.gene035837.t1 AT1G35160 77.740 292 33 3 1 260 1 292 3.38e-161 449
MS.gene035837.t1 AT4G09000 89.344 244 24 1 1 242 1 244 2.60e-160 447
MS.gene035837.t1 AT5G38480 81.496 254 46 1 6 258 2 255 3.17e-154 429
MS.gene035837.t1 AT3G02520 80.784 255 48 1 6 259 3 257 1.29e-152 426
MS.gene035837.t1 AT3G02520 80.784 255 48 1 6 259 3 257 1.29e-152 426
MS.gene035837.t1 AT5G38480 81.496 254 45 2 6 258 2 254 9.76e-152 423
MS.gene035837.t1 AT5G38480 81.496 254 45 2 6 258 2 254 9.76e-152 423
MS.gene035837.t1 AT5G10450 79.508 244 49 1 4 246 3 246 3.57e-145 406
MS.gene035837.t1 AT5G10450 79.668 241 48 1 4 243 3 243 8.26e-145 405
MS.gene035837.t1 AT5G10450 77.510 249 55 1 4 251 3 251 8.57e-145 406
MS.gene035837.t1 AT5G10450 80.000 240 47 1 4 242 3 242 5.42e-144 404
MS.gene035837.t1 AT5G16050 79.310 261 50 2 3 260 2 261 3.21e-143 402
MS.gene035837.t1 AT5G16050 79.310 261 50 2 3 260 2 261 3.21e-143 402
MS.gene035837.t1 AT5G65430 78.138 247 52 2 1 246 1 246 2.10e-142 399
MS.gene035837.t1 AT5G65430 78.279 244 51 2 1 243 1 243 5.92e-142 398
MS.gene035837.t1 AT5G65430 78.601 243 50 2 1 242 1 242 2.72e-141 397
MS.gene035837.t1 AT1G26480 74.180 244 60 3 8 250 10 251 7.27e-129 366
MS.gene035837.t1 AT2G42590 70.539 241 69 1 8 248 7 245 3.23e-122 349
MS.gene035837.t1 AT2G42590 70.539 241 69 1 8 248 7 245 3.25e-122 349
MS.gene035837.t1 AT2G42590 70.833 240 68 1 8 247 7 244 8.79e-122 348
MS.gene035837.t1 AT1G34760 71.008 238 67 1 8 245 5 240 3.66e-121 346
MS.gene035837.t1 AT1G34760 71.008 238 67 1 8 245 5 240 4.58e-121 346
MS.gene035837.t1 AT1G22300 68.254 252 73 2 8 259 5 249 6.51e-117 335
MS.gene035837.t1 AT1G22300 68.254 252 73 2 8 259 5 249 6.51e-117 335
MS.gene035837.t1 AT1G22300 69.874 239 70 1 8 246 5 241 1.34e-115 332
MS.gene035837.t1 AT1G78220 48.927 233 116 2 8 239 5 235 1.26e-73 225
MS.gene035837.t1 AT1G22290 45.933 209 91 6 12 217 9 198 4.49e-48 159
MS.gene035837.t1 AT1G22290 45.631 206 90 6 12 214 9 195 6.16e-48 157
MS.gene035837.t1 AT2G10450 71.429 49 14 0 207 255 16 64 7.67e-18 76.3

Find 0 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTTAATTAGGTTAAAATAA+TGG - 37512:2896-2915 MS.gene035837:intron 15.0%
!!! ATTTTGGTTTATATTTTGTT+TGG - 37512:4026-4045 MS.gene035837:CDS 15.0%
!!! TAATATGATTTGTGTTAATT+AGG - 37512:2784-2803 MS.gene035837:intron 15.0%
!!! TTTTGGTTTATATTTTGTTT+GGG - 37512:4027-4046 MS.gene035837:CDS 15.0%
!! AAAATGAAAGTCTTAAATGA+TGG - 37512:2983-3002 MS.gene035837:intron 20.0%
!! AATTCACATCAACATTAAAA+AGG + 37512:3685-3704 None:intergenic 20.0%
!! AATTTCATTAACATGTTTGT+GGG - 37512:3639-3658 MS.gene035837:CDS 20.0%
!! ACTACAAACTTTAATTGAAA+AGG + 37512:3478-3497 None:intergenic 20.0%
!! TAATGAATATAGAAAAGAAC+AGG + 37512:3823-3842 None:intergenic 20.0%
!! TAATTTCATTAACATGTTTG+TGG - 37512:3638-3657 MS.gene035837:CDS 20.0%
!!! CATATTCTGTTTATTGATTT+TGG - 37512:4010-4029 MS.gene035837:CDS 20.0%
!!! GATTTGATGATTTCTTAATT+AGG - 37512:2884-2903 MS.gene035837:CDS 20.0%
!!! TGTTCTTTTCTATATTCATT+AGG - 37512:3822-3841 MS.gene035837:CDS 20.0%
!!! TTTTCAATTAAAGTTTGTAG+TGG - 37512:3477-3496 MS.gene035837:intron 20.0%
! ACTACAGACTTTAATTGAAA+AGG + 37512:3539-3558 None:intergenic 25.0%
! GAGATCTAACGTTATATTTA+TGG - 37512:2585-2604 MS.gene035837:intron 25.0%
! TATTCTAGATACCGAATAAT+GGG - 37512:3411-3430 MS.gene035837:intron 25.0%
! TTTAGGTAAGGGAAATTTAA+CGG - 37512:2728-2747 MS.gene035837:intron 25.0%
!! AATGTTGATGTGAATTTGTT+AGG - 37512:3689-3708 MS.gene035837:CDS 25.0%
!! TGATTCTGTTCTGTTATTTA+TGG - 37512:3777-3796 MS.gene035837:CDS 25.0%
!! TTTCAAATGAGTTGAATGTA+GGG - 37512:2941-2960 MS.gene035837:intron 25.0%
!! TTTTCAATTAAAGTCTGTAG+TGG - 37512:3538-3557 MS.gene035837:intron 25.0%
!!! TGTAGGGTATTGTTTATTTT+TGG - 37512:2957-2976 MS.gene035837:intron 25.0%
!!! TTATATTTTGTTTGGGTTTC+AGG - 37512:4034-4053 MS.gene035837:CDS 25.0%
!!! TTTAATTTGACTCTGTTTGT+AGG - 37512:3261-3280 MS.gene035837:intron 25.0%
!!! TTTTCAAATGAGTTGAATGT+AGG - 37512:2940-2959 MS.gene035837:intron 25.0%
AAGAATACCGTTACAGATAT+TGG + 37512:2384-2403 None:intergenic 30.0%
AGATGAAAGGTGATTATCAT+AGG - 37512:2468-2487 MS.gene035837:intron 30.0%
ATTGCTGAATTGGATACATT+GGG - 37512:3859-3878 MS.gene035837:CDS 30.0%
CGCAATATTTCAAACAAGAA+TGG + 37512:2695-2714 None:intergenic 30.0%
GAACTTTCCAATATCTGTAA+CGG - 37512:2374-2393 MS.gene035837:intron 30.0%
GATAATCACCTTTCATCTTA+AGG + 37512:2466-2485 None:intergenic 30.0%
GTATTCTAGATACCGAATAA+TGG - 37512:3410-3429 MS.gene035837:intron 30.0%
TATTGCTGAATTGGATACAT+TGG - 37512:3858-3877 MS.gene035837:CDS 30.0%
TATTTGTTCCTCAACAAGTT+TGG - 37512:3741-3760 MS.gene035837:CDS 30.0%
TTAGATCTCAGAGAAAAAGA+TGG + 37512:2574-2593 None:intergenic 30.0%
! CTATAACTGACTTTAGGTAA+GGG - 37512:2717-2736 MS.gene035837:intron 30.0%
! GTTTTCTACCTTAAGATGAA+AGG - 37512:2455-2474 MS.gene035837:intron 30.0%
! TCTATAACTGACTTTAGGTA+AGG - 37512:2716-2735 MS.gene035837:intron 30.0%
! TTTAACGGAGTTTTTGTGTT+TGG - 37512:2743-2762 MS.gene035837:intron 30.0%
AATGGGAGATGATGTTCATT+TGG - 37512:3428-3447 MS.gene035837:intron 35.0%
AGATCTGTAATCACGAATCA+CGG + 37512:2342-2361 None:intergenic 35.0%
ATATTGACACCTCTGATCAA+AGG - 37512:3110-3129 MS.gene035837:intron 35.0%
GAACATCATCTCCCATTATT+CGG + 37512:3425-3444 None:intergenic 35.0%
TAACATGTTCATCGTTACCA+CGG + 37512:2319-2338 None:intergenic 35.0%
TATCTTGCTGAGTTTAAGAG+TGG - 37512:2491-2510 MS.gene035837:intron 35.0%
TATTCATTAGGCTTTCGATG+AGG - 37512:3834-3853 MS.gene035837:CDS 35.0%
TTGAACATAATCATGTGCGT+CGG + 37512:3159-3178 None:intergenic 35.0%
! GAATACTAACCTGTTTTGCA+AGG + 37512:3396-3415 None:intergenic 35.0%
! GTGTTATTATTGCGTCAGTT+TGG - 37512:3214-3233 MS.gene035837:intron 35.0%
! TGCGATCTATAACTGACTTT+AGG - 37512:2711-2730 MS.gene035837:intron 35.0%
!! GATGTGAATTTGTTAGGTTC+TGG - 37512:3695-3714 MS.gene035837:CDS 35.0%
ATATCATACCTGCATGTCAG+AGG + 37512:3950-3969 None:intergenic 40.0%
ATTGGGAGAGGAATCATACA+AGG - 37512:3876-3895 MS.gene035837:CDS 40.0%
TACTGCTTATAAAGCTGCTC+AGG - 37512:2547-2566 MS.gene035837:intron 40.0%
TGAATTGGATACATTGGGAG+AGG - 37512:3864-3883 MS.gene035837:CDS 40.0%
! TTGTTTGGGTTTCAGGATGA+TGG - 37512:4041-4060 MS.gene035837:CDS 40.0%
!! ATGCTACACCAAACTTGTTG+AGG + 37512:3752-3771 None:intergenic 40.0%
ATCAAAGAAGCCGCACCAAA+AGG - 37512:4074-4093 MS.gene035837:CDS 45.0%
ATGTAGACGAACTCTTCACG+CGG + 37512:2104-2123 None:intergenic 45.0%
CATCTCTTCCATCGAACAGA+AGG - 37512:2286-2305 MS.gene035837:CDS 45.0%
CGATGAGGCTATTGCTGAAT+TGG - 37512:3849-3868 MS.gene035837:CDS 45.0%
GACCATCTCTTCGTAACGTT+CGG + 37512:2147-2166 None:intergenic 45.0%
GCTTTCTTCCTTCTGTTCGA+TGG + 37512:2297-2316 None:intergenic 45.0%
GTCGCCTACAAAAACGTCAT+CGG - 37512:2239-2258 MS.gene035837:CDS 45.0%
TCCAAAGGGTGAGGTTATCA+CGG + 37512:3927-3946 None:intergenic 45.0%
! CGAAGAGATGGTCGATTTCA+TGG - 37512:2154-2173 MS.gene035837:intron 45.0%
! GATGACGTTTTTGTAGGCGA+CGG + 37512:2240-2259 None:intergenic 45.0%
! TGCTCCGATGACGTTTTTGT+AGG + 37512:2246-2265 None:intergenic 45.0%
! TGCTGCTTCTGGTGATTCTA+AGG - 37512:2433-2452 MS.gene035837:intron 45.0%
! TTCGTAACGTTCGGCTTGTT+CGG + 37512:2138-2157 None:intergenic 45.0%
! TTGATTCCTTCTGCTGCTTC+TGG - 37512:2422-2441 MS.gene035837:intron 45.0%
AACTCTCCTGATCGTGCTTG+TGG - 37512:3370-3389 MS.gene035837:intron 50.0%
AGAGCAAGACCAAGCCTGAT+TGG + 37512:3319-3338 None:intergenic 50.0%
AGCCGAACGTTACGAAGAGA+TGG - 37512:2142-2161 MS.gene035837:intron 50.0%
CTGCATGTCAGAGGTCCAAA+GGG + 37512:3941-3960 None:intergenic 50.0%
GAACAGAAGGAAGAAAGCCG+TGG - 37512:2299-2318 MS.gene035837:CDS 50.0%
GAATCACCAGAAGCAGCAGA+AGG + 37512:2431-2450 None:intergenic 50.0%
GAGCAAGACCAAGCCTGATT+GGG + 37512:3318-3337 None:intergenic 50.0%
GAGGAGGTTACGTTCTTCGA+CGG + 37512:2216-2235 None:intergenic 50.0%
GCGTGAAGAGTTCGTCTACA+TGG - 37512:2103-2122 MS.gene035837:CDS 50.0%
GGTGAGTTCTTCGCTTTCGA+CGG + 37512:2195-2214 None:intergenic 50.0%
TACCACCAACTCACCCAATC+AGG - 37512:3302-3321 MS.gene035837:intron 50.0%
TCCGTGATAACCTCACCCTT+TGG - 37512:3923-3942 MS.gene035837:CDS 50.0%
! TGCTTGTGGCCTTGCAAAAC+AGG - 37512:3384-3403 MS.gene035837:intron 50.0%
! TGTGGGAGCTTTAGTGCAGT+GGG - 37512:3656-3675 MS.gene035837:CDS 50.0%
! TTGTGGGAGCTTTAGTGCAG+TGG - 37512:3655-3674 MS.gene035837:CDS 50.0%
ACGAACTCTTCACGCGGTGT+TGG + 37512:2098-2117 None:intergenic 55.0%
CCAACTCACCCAATCAGGCT+TGG - 37512:3307-3326 MS.gene035837:intron 55.0%
CCAAGCCTGATTGGGTGAGT+TGG + 37512:3310-3329 None:intergenic 55.0%
CCAGACACGCCTTTGATCAG+AGG + 37512:3122-3141 None:intergenic 55.0%
CCTGCATGTCAGAGGTCCAA+AGG + 37512:3942-3961 None:intergenic 55.0%
CCTTTGGACCTCTGACATGC+AGG - 37512:3939-3958 MS.gene035837:CDS 55.0%
GAGTTCTTCGCTTTCGACGG+CGG + 37512:2192-2211 None:intergenic 55.0%
TGTCAGAGGTCCAAAGGGTG+AGG + 37512:3936-3955 None:intergenic 55.0%
! AGCCTGATTGGGTGAGTTGG+TGG + 37512:3307-3326 None:intergenic 55.0%
! AGGCGTGTCTGGTTTCTGAC+AGG - 37512:3130-3149 MS.gene035837:intron 55.0%
! CCTCTGATCAAAGGCGTGTC+TGG - 37512:3119-3138 MS.gene035837:intron 55.0%
! CGTTTTTGTAGGCGACGGAG+AGG + 37512:2235-2254 None:intergenic 55.0%
! GCGTGTCTGGTTTCTGACAG+GGG - 37512:3132-3151 MS.gene035837:intron 55.0%
! GGCGTGTCTGGTTTCTGACA+GGG - 37512:3131-3150 MS.gene035837:intron 55.0%
! TACTGCTCATCGGCGCCTTT+TGG + 37512:4092-4111 None:intergenic 55.0%
! TTTTGTAGGCGACGGAGAGG+AGG + 37512:2232-2251 None:intergenic 55.0%
GCAAGGCCACAAGCACGATC+AGG + 37512:3379-3398 None:intergenic 60.0%
TCGGAGCAAGACGTGCTTCG+TGG - 37512:2258-2277 MS.gene035837:CDS 60.0%
TTCTTCGCTTTCGACGGCGG+CGG + 37512:2189-2208 None:intergenic 60.0%
! CTCATCGGCGCCTTTTGGTG+CGG + 37512:4087-4106 None:intergenic 60.0%
!! CTCTTCACGCGGTGTTGGTG+CGG + 37512:2093-2112 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
37512 gene 2083 4112 2083 ID=MS.gene035837
37512 mRNA 2083 4112 2083 ID=MS.gene035837.t1;Parent=MS.gene035837
37512 exon 3627 4112 3627 ID=MS.gene035837.t1.exon1;Parent=MS.gene035837.t1
37512 CDS 3627 4112 3627 ID=cds.MS.gene035837.t1;Parent=MS.gene035837.t1
37512 exon 2790 2912 2790 ID=MS.gene035837.t1.exon2;Parent=MS.gene035837.t1
37512 CDS 2790 2912 2790 ID=cds.MS.gene035837.t1;Parent=MS.gene035837.t1
37512 exon 2235 2351 2235 ID=MS.gene035837.t1.exon3;Parent=MS.gene035837.t1
37512 CDS 2235 2351 2235 ID=cds.MS.gene035837.t1;Parent=MS.gene035837.t1
37512 exon 2083 2139 2083 ID=MS.gene035837.t1.exon4;Parent=MS.gene035837.t1
37512 CDS 2083 2139 2083 ID=cds.MS.gene035837.t1;Parent=MS.gene035837.t1
Gene Sequence

>MS.gene035837

ATGGCAACCGCACCAACACCGCGTGAAGAGTTCGTCTACATGGCGAAACTCGCCGAACAAGCCGAACGTTACGAAGAGATGGTCGATTTCATGGAAAAAGTAACCGCCGCCGTCGAAAGCGAAGAACTCACCGTCGAAGAACGTAACCTCCTCTCCGTCGCCTACAAAAACGTCATCGGAGCAAGACGTGCTTCGTGGCGTATCATCTCTTCCATCGAACAGAAGGAAGAAAGCCGTGGTAACGATGAACATGTTACCGTGATTCGTGATTACAGATCTAAGATCGAAGCTGAACTTTCCAATATCTGTAACGGTATTCTTAAGCTTCTTGATTCTCGCTTGATTCCTTCTGCTGCTTCTGGTGATTCTAAGGTTTTCTACCTTAAGATGAAAGGTGATTATCATAGGTATCTTGCTGAGTTTAAGAGTGGCGCTGAGCGTAAAGATGCTGCTGAAAGTACTCTTACTGCTTATAAAGCTGCTCAGGTCCATCTTTTTCTCTGAGATCTAACGTTATATTTATGGATCTGTTTTTTCAATTATTTTTTCATTTTTTATTTTTATTTTGTTGTTATTTCATTAATTATTGTTAATTTGATGATTTAGATTCCATTCTTGTTTGAAATATTGCGATCTATAACTGACTTTAGGTAAGGGAAATTTAACGGAGTTTTTGTGTTTGGTGTTTTTTCATGAAAAATTAATATGATTTGTGTTAATTAGGTTTTTTGTTTAATGTAATGATTTTTTTAGTGATGTGATTTATTTATCTTTTAGAGAAAATGATGTAAGCATGCACTTGATTTGATGATTTCTTAATTAGGTTAAAATAATGGAATCTCTATGATAAAAGTGATTTTTCAAATGAGTTGAATGTAGGGTATTGTTTATTTTTGGTTTAAAATGAAAGTCTTAAATGATGGAACGTGTGCTAGTTATTTTACTTGTTTAGACTATTTGATTATGATGATATGCATGTTTTGTGTTTTTTGCTTGTTTAAAGCGTTTATAGTTTTCGAATCTTTGTATATTGACACCTCTGATCAAAGGCGTGTCTGGTTTCTGACAGGGGACCGACGCACATGATTATGTTCAATTAAATTCATTATCTCAATTTTGACTGCTGTTGTTGTGTTATTATTGCGTCAGTTTGGATTTGAAGATTTGCGATCTGATGTTTTAATTTGACTCTGTTTGTAGGACATTGCTAACTCTGAGCTACCACCAACTCACCCAATCAGGCTTGGTCTTGCTCTGAACTTCTCAGTGTTTTACTATGAGATTCTTAACTCTCCTGATCGTGCTTGTGGCCTTGCAAAACAGGTTAGTATTCTAGATACCGAATAATGGGAGATGATGTTCATTTGGTTTGTTGTTTCTTTCAATATGTTTCCTTTTCAATTAAAGTTTGTAGTGGATTTTGTGAAAAAGTATGTGAATTCTGTTTATCACACCTTTTCAATTAAAGTCTGTAGTGGATTTTGTGAAAAAGTATGTGAATTCTGTTTAATGTGAATTAGTTTGATGTGTATTTCAATCTATGTTTTAGTTTCATTAATTTCATTAACATGTTTGTGGGAGCTTTAGTGCAGTGGGCTGCCTTTTTAATGTTGATGTGAATTTGTTAGGTTCTGGTAAAATACATACGAAACTTTTAATATTTGTTCCTCAACAAGTTTGGTGTAGCATGTACATGATTCTGTTCTGTTATTTATGGTGCATGTGCTAATAAACAATCCTGTTCTTTTCTATATTCATTAGGCTTTCGATGAGGCTATTGCTGAATTGGATACATTGGGAGAGGAATCATACAAGGACAGCACTTTGATCATGCAACTTCTCCGTGATAACCTCACCCTTTGGACCTCTGACATGCAGGTATGATATTTTGTATTTTGTAATCTCTTAATTAACTTTTACTTGCAATCATATTCTGTTTATTGATTTTGGTTTATATTTTGTTTGGGTTTCAGGATGATGGTGCAGATGAAATCAAAGAAGCCGCACCAAAAGGCGCCGATGAGCAGTAA

Protein sequence

>MS.gene035837.t1

MATAPTPREEFVYMAKLAEQAERYEEMVDFMEKVTAAVESEELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEESRGNDEHVTVIRDYRSKIEAELSNICNGILKLLDSRLIPSAASGDSKVFYLKMKGDYHRYLAEFKSGAERKDAAESTLTAYKAAQDIANSELPPTHPIRLGLALNFSVFYYEILNSPDRACGLAKQAFDEAIAELDTLGEESYKDSTLIMQLLRDNLTLWTSDMQDDGADEIKEAAPKGADEQ