Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48435.t1 | XP_003602941.1 | 100 | 252 | 0 | 0 | 1 | 252 | 1 | 252 | 1.30E-133 | 485.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48435.t1 | Q96451 | 89.0 | 246 | 27 | 0 | 7 | 252 | 2 | 247 | 1.5e-119 | 430.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48435.t1 | G7J567 | 100.0 | 252 | 0 | 0 | 1 | 252 | 1 | 252 | 9.2e-134 | 485.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049230 | MS.gene48435 | 0.847942 | 8.40E-60 | -1.69E-46 |
| MS.gene049297 | MS.gene48435 | 0.832379 | 9.78E-56 | -1.69E-46 |
| MS.gene04933 | MS.gene48435 | 0.808814 | 2.57E-50 | -1.69E-46 |
| MS.gene049378 | MS.gene48435 | 0.890352 | 1.06E-73 | -1.69E-46 |
| MS.gene049424 | MS.gene48435 | 0.821561 | 3.78E-53 | -1.69E-46 |
| MS.gene049669 | MS.gene48435 | 0.827309 | 1.68E-54 | -1.69E-46 |
| MS.gene049758 | MS.gene48435 | 0.800945 | 1.14E-48 | -1.69E-46 |
| MS.gene049910 | MS.gene48435 | 0.803308 | 3.71E-49 | -1.69E-46 |
| MS.gene050226 | MS.gene48435 | 0.866798 | 2.19E-65 | -1.69E-46 |
| MS.gene050227 | MS.gene48435 | 0.881405 | 2.47E-70 | -1.69E-46 |
| MS.gene050228 | MS.gene48435 | 0.875308 | 3.41E-68 | -1.69E-46 |
| MS.gene050303 | MS.gene48435 | 0.825118 | 5.58E-54 | -1.69E-46 |
| MS.gene050330 | MS.gene48435 | -0.873673 | 1.23E-67 | -1.69E-46 |
| MS.gene050442 | MS.gene48435 | 0.818414 | 1.98E-52 | -1.69E-46 |
| MS.gene050558 | MS.gene48435 | 0.818964 | 1.49E-52 | -1.69E-46 |
| MS.gene050623 | MS.gene48435 | -0.854439 | 1.23E-61 | -1.69E-46 |
| MS.gene050642 | MS.gene48435 | 0.879617 | 1.08E-69 | -1.69E-46 |
| MS.gene050719 | MS.gene48435 | 0.845722 | 3.40E-59 | -1.69E-46 |
| MS.gene050721 | MS.gene48435 | 0.848306 | 6.67E-60 | -1.69E-46 |
| MS.gene050724 | MS.gene48435 | 0.838694 | 2.47E-57 | -1.69E-46 |
| MS.gene050750 | MS.gene48435 | 0.828356 | 9.41E-55 | -1.69E-46 |
| MS.gene050780 | MS.gene48435 | 0.814383 | 1.58E-51 | -1.69E-46 |
| MS.gene050820 | MS.gene48435 | 0.83751 | 4.98E-57 | -1.69E-46 |
| MS.gene050824 | MS.gene48435 | 0.805551 | 1.26E-49 | -1.69E-46 |
| MS.gene050953 | MS.gene48435 | 0.825928 | 3.59E-54 | -1.69E-46 |
| MS.gene050987 | MS.gene48435 | 0.835137 | 2.00E-56 | -1.69E-46 |
| MS.gene051064 | MS.gene48435 | 0.821821 | 3.29E-53 | -1.69E-46 |
| MS.gene05119 | MS.gene48435 | -0.815061 | 1.12E-51 | -1.69E-46 |
| MS.gene051257 | MS.gene48435 | 0.804676 | 1.93E-49 | -1.69E-46 |
| MS.gene051349 | MS.gene48435 | 0.83629 | 1.02E-56 | -1.69E-46 |
| MS.gene051633 | MS.gene48435 | 0.818858 | 1.57E-52 | -1.69E-46 |
| MS.gene051983 | MS.gene48435 | 0.859161 | 5.01E-63 | -1.69E-46 |
| MS.gene052077 | MS.gene48435 | 0.833651 | 4.72E-56 | -1.69E-46 |
| MS.gene052176 | MS.gene48435 | 0.999155 | 4.52E-293 | -1.69E-46 |
| MS.gene052286 | MS.gene48435 | 0.808241 | 3.41E-50 | -1.69E-46 |
| MS.gene052302 | MS.gene48435 | 0.879915 | 8.43E-70 | -1.69E-46 |
| MS.gene052309 | MS.gene48435 | -0.865647 | 5.07E-65 | -1.69E-46 |
| MS.gene052333 | MS.gene48435 | 0.816686 | 4.87E-52 | -1.69E-46 |
| MS.gene052358 | MS.gene48435 | -0.855305 | 6.90E-62 | -1.69E-46 |
| MS.gene052376 | MS.gene48435 | 0.884736 | 1.49E-71 | -1.69E-46 |
| MS.gene052389 | MS.gene48435 | 0.900544 | 6.59E-78 | -1.69E-46 |
| MS.gene052586 | MS.gene48435 | 0.820951 | 5.23E-53 | -1.69E-46 |
| MS.gene052600 | MS.gene48435 | 0.839563 | 1.47E-57 | -1.69E-46 |
| MS.gene052856 | MS.gene48435 | 0.839417 | 1.60E-57 | -1.69E-46 |
| MS.gene052858 | MS.gene48435 | 0.840261 | 9.67E-58 | -1.69E-46 |
| MS.gene052860 | MS.gene48435 | 0.834758 | 2.49E-56 | -1.69E-46 |
| MS.gene053188 | MS.gene48435 | 0.823694 | 1.21E-53 | -1.69E-46 |
| MS.gene053216 | MS.gene48435 | 0.831842 | 1.33E-55 | -1.69E-46 |
| MS.gene053836 | MS.gene48435 | 0.834724 | 2.54E-56 | -1.69E-46 |
| MS.gene054160 | MS.gene48435 | 0.814915 | 1.21E-51 | -1.69E-46 |
| MS.gene054291 | MS.gene48435 | -0.802448 | 5.58E-49 | -1.69E-46 |
| MS.gene054379 | MS.gene48435 | 0.80835 | 3.23E-50 | -1.69E-46 |
| MS.gene054405 | MS.gene48435 | 0.81202 | 5.23E-51 | -1.69E-46 |
| MS.gene054468 | MS.gene48435 | 0.838829 | 2.28E-57 | -1.69E-46 |
| MS.gene054537 | MS.gene48435 | 0.82222 | 2.66E-53 | -1.69E-46 |
| MS.gene054542 | MS.gene48435 | 0.80554 | 1.27E-49 | -1.69E-46 |
| MS.gene054930 | MS.gene48435 | 0.883941 | 2.93E-71 | -1.69E-46 |
| MS.gene054942 | MS.gene48435 | 0.842059 | 3.26E-58 | -1.69E-46 |
| MS.gene054983 | MS.gene48435 | 0.828331 | 9.54E-55 | -1.69E-46 |
| MS.gene05510 | MS.gene48435 | 0.803653 | 3.15E-49 | -1.69E-46 |
| MS.gene055225 | MS.gene48435 | 0.804902 | 1.73E-49 | -1.69E-46 |
| MS.gene055367 | MS.gene48435 | 0.800808 | 1.21E-48 | -1.69E-46 |
| MS.gene055701 | MS.gene48435 | 0.837193 | 6.01E-57 | -1.69E-46 |
| MS.gene05595 | MS.gene48435 | 0.800991 | 1.11E-48 | -1.69E-46 |
| MS.gene056069 | MS.gene48435 | -0.824166 | 9.35E-54 | -1.69E-46 |
| MS.gene056087 | MS.gene48435 | 0.853856 | 1.81E-61 | -1.69E-46 |
| MS.gene056506 | MS.gene48435 | 0.860801 | 1.60E-63 | -1.69E-46 |
| MS.gene056979 | MS.gene48435 | 0.846238 | 2.46E-59 | -1.69E-46 |
| MS.gene057026 | MS.gene48435 | 0.839153 | 1.88E-57 | -1.69E-46 |
| MS.gene057060 | MS.gene48435 | 0.812588 | 3.93E-51 | -1.69E-46 |
| MS.gene057079 | MS.gene48435 | 0.837823 | 4.14E-57 | -1.69E-46 |
| MS.gene057200 | MS.gene48435 | 0.800443 | 1.44E-48 | -1.69E-46 |
| MS.gene057555 | MS.gene48435 | 0.804904 | 1.73E-49 | -1.69E-46 |
| MS.gene05761 | MS.gene48435 | 0.828252 | 9.97E-55 | -1.69E-46 |
| MS.gene05795 | MS.gene48435 | 0.846283 | 2.39E-59 | -1.69E-46 |
| MS.gene057967 | MS.gene48435 | 0.832742 | 7.95E-56 | -1.69E-46 |
| MS.gene058139 | MS.gene48435 | 0.836224 | 1.06E-56 | -1.69E-46 |
| MS.gene058142 | MS.gene48435 | 0.816812 | 4.56E-52 | -1.69E-46 |
| MS.gene058182 | MS.gene48435 | 0.831277 | 1.83E-55 | -1.69E-46 |
| MS.gene058200 | MS.gene48435 | 0.844309 | 8.19E-59 | -1.69E-46 |
| MS.gene058201 | MS.gene48435 | 0.80624 | 9.05E-50 | -1.69E-46 |
| MS.gene058238 | MS.gene48435 | 0.811088 | 8.34E-51 | -1.69E-46 |
| MS.gene058378 | MS.gene48435 | 0.818496 | 1.90E-52 | -1.69E-46 |
| MS.gene058399 | MS.gene48435 | 0.809337 | 1.99E-50 | -1.69E-46 |
| MS.gene058498 | MS.gene48435 | 0.802051 | 6.74E-49 | -1.69E-46 |
| MS.gene058710 | MS.gene48435 | 0.83679 | 7.61E-57 | -1.69E-46 |
| MS.gene058749 | MS.gene48435 | 0.84837 | 6.40E-60 | -1.69E-46 |
| MS.gene058833 | MS.gene48435 | 0.81811 | 2.33E-52 | -1.69E-46 |
| MS.gene059128 | MS.gene48435 | 0.814353 | 1.61E-51 | -1.69E-46 |
| MS.gene059247 | MS.gene48435 | 0.834204 | 3.43E-56 | -1.69E-46 |
| MS.gene059249 | MS.gene48435 | 0.802496 | 5.46E-49 | -1.69E-46 |
| MS.gene059306 | MS.gene48435 | 0.802348 | 5.86E-49 | -1.69E-46 |
| MS.gene059317 | MS.gene48435 | 0.802672 | 5.02E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene48435 | 0.800585 | 1.35E-48 | -1.69E-46 |
| MS.gene059710 | MS.gene48435 | 0.821147 | 4.71E-53 | -1.69E-46 |
| MS.gene05984 | MS.gene48435 | 0.803148 | 4.00E-49 | -1.69E-46 |
| MS.gene060084 | MS.gene48435 | 0.806197 | 9.24E-50 | -1.69E-46 |
| MS.gene060165 | MS.gene48435 | 0.854883 | 9.15E-62 | -1.69E-46 |
| MS.gene060297 | MS.gene48435 | 0.854421 | 1.25E-61 | -1.69E-46 |
| MS.gene060322 | MS.gene48435 | 0.81483 | 1.26E-51 | -1.69E-46 |
| MS.gene060572 | MS.gene48435 | 0.826625 | 2.45E-54 | -1.69E-46 |
| MS.gene060619 | MS.gene48435 | 0.848414 | 6.22E-60 | -1.69E-46 |
| MS.gene060683 | MS.gene48435 | 0.830293 | 3.19E-55 | -1.69E-46 |
| MS.gene060685 | MS.gene48435 | 0.830038 | 3.68E-55 | -1.69E-46 |
| MS.gene060879 | MS.gene48435 | 0.847736 | 9.58E-60 | -1.69E-46 |
| MS.gene060998 | MS.gene48435 | 0.806489 | 8.02E-50 | -1.69E-46 |
| MS.gene061040 | MS.gene48435 | -0.887245 | 1.69E-72 | -1.69E-46 |
| MS.gene061114 | MS.gene48435 | -0.822689 | 2.07E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene48435 | MS.gene050079 | PPI |
| MS.gene48435 | MS.gene38593 | PPI |
| MS.gene48435 | MS.gene020411 | PPI |
| MS.gene48435 | MS.gene53188 | PPI |
| MS.gene48435 | MS.gene043907 | PPI |
| MS.gene48435 | MS.gene55401 | PPI |
| MS.gene55400 | MS.gene48435 | PPI |
| MS.gene48435 | MS.gene010179 | PPI |
| MS.gene020411 | MS.gene48435 | PPI |
| MS.gene48435 | MS.gene054662 | PPI |
| MS.gene48435 | MS.gene055488 | PPI |
| MS.gene48435 | MS.gene010180 | PPI |
| MS.gene48435 | MS.gene028807 | PPI |
| MS.gene006850 | MS.gene48435 | PPI |
| MS.gene48435 | MS.gene035837 | PPI |
| MS.gene48435 | MS.gene043514 | PPI |
| MS.gene48435 | MS.gene052176 | PPI |
| MS.gene066779 | MS.gene48435 | PPI |
| MS.gene48435 | MS.gene31370 | PPI |
| MS.gene48435 | MS.gene013133 | PPI |
| MS.gene54847 | MS.gene48435 | PPI |
| MS.gene48435 | MS.gene013047 | PPI |
| MS.gene48435 | MS.gene56475 | PPI |
| MS.gene48435 | MS.gene64150 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48435.t1 | MTR_3g100620 | 100.000 | 252 | 0 | 0 | 1 | 252 | 1 | 252 | 0.0 | 515 |
| MS.gene48435.t1 | MTR_3g099380 | 76.793 | 237 | 54 | 1 | 11 | 247 | 8 | 243 | 1.45e-135 | 382 |
| MS.gene48435.t1 | MTR_5g064580 | 75.527 | 237 | 57 | 1 | 11 | 247 | 8 | 243 | 8.20e-135 | 380 |
| MS.gene48435.t1 | MTR_0137s0020 | 73.413 | 252 | 62 | 3 | 1 | 251 | 1 | 248 | 6.79e-127 | 360 |
| MS.gene48435.t1 | MTR_3g014060 | 73.016 | 252 | 63 | 3 | 1 | 251 | 1 | 248 | 6.63e-126 | 357 |
| MS.gene48435.t1 | MTR_2g091225 | 65.289 | 242 | 81 | 1 | 10 | 251 | 6 | 244 | 8.22e-116 | 332 |
| MS.gene48435.t1 | MTR_8g086270 | 65.145 | 241 | 81 | 1 | 11 | 251 | 7 | 244 | 1.16e-113 | 327 |
| MS.gene48435.t1 | MTR_5g044160 | 66.809 | 235 | 75 | 1 | 11 | 245 | 7 | 238 | 2.79e-113 | 326 |
| MS.gene48435.t1 | MTR_4g083060 | 66.383 | 235 | 76 | 1 | 11 | 245 | 7 | 238 | 3.28e-112 | 324 |
| MS.gene48435.t1 | MTR_2g015980 | 64.557 | 237 | 80 | 2 | 12 | 248 | 6 | 238 | 3.87e-109 | 315 |
| MS.gene48435.t1 | MTR_2g076960 | 64.681 | 235 | 80 | 1 | 11 | 245 | 8 | 239 | 8.02e-109 | 315 |
| MS.gene48435.t1 | MTR_3g099380 | 73.096 | 197 | 52 | 1 | 11 | 207 | 8 | 203 | 1.61e-105 | 304 |
| MS.gene48435.t1 | MTR_2g091225 | 67.476 | 206 | 67 | 0 | 46 | 251 | 16 | 221 | 1.19e-103 | 300 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48435.t1 | AT5G10450 | 84.016 | 244 | 39 | 0 | 8 | 251 | 4 | 247 | 1.17e-155 | 432 |
| MS.gene48435.t1 | AT5G65430 | 82.787 | 244 | 42 | 0 | 8 | 251 | 4 | 247 | 2.84e-154 | 429 |
| MS.gene48435.t1 | AT5G10450 | 84.519 | 239 | 37 | 0 | 8 | 246 | 4 | 242 | 8.08e-153 | 425 |
| MS.gene48435.t1 | AT5G10450 | 84.519 | 239 | 37 | 0 | 8 | 246 | 4 | 242 | 2.77e-152 | 424 |
| MS.gene48435.t1 | AT5G10450 | 84.519 | 239 | 37 | 0 | 8 | 246 | 4 | 242 | 3.77e-152 | 425 |
| MS.gene48435.t1 | AT5G65430 | 83.264 | 239 | 40 | 0 | 8 | 246 | 4 | 242 | 2.58e-151 | 421 |
| MS.gene48435.t1 | AT5G65430 | 83.264 | 239 | 40 | 0 | 8 | 246 | 4 | 242 | 1.05e-150 | 421 |
| MS.gene48435.t1 | AT1G35160 | 73.950 | 238 | 61 | 1 | 11 | 248 | 11 | 247 | 1.52e-132 | 375 |
| MS.gene48435.t1 | AT1G78300 | 73.950 | 238 | 61 | 1 | 10 | 247 | 4 | 240 | 5.99e-132 | 373 |
| MS.gene48435.t1 | AT4G09000 | 73.222 | 239 | 63 | 1 | 11 | 249 | 10 | 247 | 1.77e-131 | 372 |
| MS.gene48435.t1 | AT3G02520 | 73.640 | 239 | 62 | 1 | 10 | 248 | 4 | 241 | 2.67e-131 | 372 |
| MS.gene48435.t1 | AT3G02520 | 73.640 | 239 | 62 | 1 | 10 | 248 | 4 | 241 | 2.67e-131 | 372 |
| MS.gene48435.t1 | AT1G35160 | 74.153 | 236 | 60 | 1 | 11 | 246 | 11 | 245 | 8.68e-131 | 372 |
| MS.gene48435.t1 | AT5G38480 | 73.222 | 239 | 63 | 1 | 10 | 248 | 3 | 240 | 1.09e-129 | 367 |
| MS.gene48435.t1 | AT5G38480 | 72.727 | 242 | 65 | 1 | 10 | 251 | 3 | 243 | 4.05e-129 | 366 |
| MS.gene48435.t1 | AT5G38480 | 72.727 | 242 | 65 | 1 | 10 | 251 | 3 | 243 | 4.05e-129 | 366 |
| MS.gene48435.t1 | AT4G09000 | 73.729 | 236 | 61 | 1 | 11 | 246 | 10 | 244 | 4.21e-129 | 368 |
| MS.gene48435.t1 | AT5G16050 | 73.640 | 239 | 62 | 1 | 10 | 248 | 6 | 243 | 2.84e-125 | 357 |
| MS.gene48435.t1 | AT5G16050 | 73.640 | 239 | 62 | 1 | 10 | 248 | 6 | 243 | 2.84e-125 | 357 |
| MS.gene48435.t1 | AT1G26480 | 67.660 | 235 | 73 | 1 | 11 | 245 | 10 | 241 | 1.44e-115 | 332 |
| MS.gene48435.t1 | AT2G42590 | 63.900 | 241 | 84 | 1 | 11 | 251 | 7 | 244 | 4.23e-108 | 313 |
| MS.gene48435.t1 | AT2G42590 | 64.286 | 238 | 82 | 1 | 11 | 248 | 7 | 241 | 9.95e-108 | 312 |
| MS.gene48435.t1 | AT2G42590 | 64.286 | 238 | 82 | 1 | 11 | 248 | 7 | 241 | 1.36e-107 | 312 |
| MS.gene48435.t1 | AT1G34760 | 63.291 | 237 | 84 | 1 | 11 | 247 | 5 | 238 | 4.34e-107 | 310 |
| MS.gene48435.t1 | AT1G34760 | 63.291 | 237 | 84 | 1 | 11 | 247 | 5 | 238 | 4.70e-107 | 310 |
| MS.gene48435.t1 | AT1G22300 | 63.485 | 241 | 85 | 1 | 11 | 251 | 5 | 242 | 1.08e-103 | 301 |
| MS.gene48435.t1 | AT1G22300 | 63.485 | 241 | 85 | 1 | 11 | 251 | 5 | 242 | 1.32e-103 | 301 |
| MS.gene48435.t1 | AT1G22300 | 63.445 | 238 | 84 | 1 | 11 | 248 | 5 | 239 | 1.81e-102 | 298 |
| MS.gene48435.t1 | AT1G78220 | 43.590 | 234 | 128 | 2 | 11 | 243 | 5 | 235 | 6.20e-62 | 195 |
| MS.gene48435.t1 | AT1G22290 | 40.000 | 210 | 105 | 5 | 14 | 221 | 8 | 198 | 5.08e-38 | 132 |
| MS.gene48435.t1 | AT1G22290 | 39.614 | 207 | 104 | 5 | 14 | 218 | 8 | 195 | 2.18e-37 | 130 |
| MS.gene48435.t1 | AT2G10450 | 68.421 | 38 | 12 | 0 | 211 | 248 | 16 | 53 | 2.15e-11 | 58.9 |
Find 46 sgRNAs with CRISPR-Local
Find 127 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATAAGTGTGCTGTCTTTGT+AGG | 0.261422 | 3.1:-79989574 | None:intergenic |
| GAATGTTTGTGGTAGTATTC+TGG | 0.294961 | 3.1:+79987739 | MS.gene48435:CDS |
| GGGTGATTATCATAGGTATT+TGG | 0.311976 | 3.1:+79987832 | MS.gene48435:CDS |
| ACACATACTGTTCTCTGTTT+AGG | 0.318322 | 3.1:-79987451 | None:intergenic |
| ATCAACTCATCCTATAAGAT+TGG | 0.331215 | 3.1:+79988031 | MS.gene48435:CDS |
| TGAGGAAGCAATTGCTGAAC+TGG | 0.348492 | 3.1:+79989533 | MS.gene48435:CDS |
| AGAGCCAAACCCAATCTTAT+AGG | 0.362825 | 3.1:-79988041 | None:intergenic |
| AGCTTGTGACATGGCCAAAC+AGG | 0.376909 | 3.1:+79988109 | MS.gene48435:CDS |
| CGTTAACTCTGATTCAACTT+TGG | 0.380898 | 3.1:-79987717 | None:intergenic |
| TCATCCTATAAGATTGGGTT+TGG | 0.386635 | 3.1:+79988037 | MS.gene48435:CDS |
| TGCTTCTTCAAGTGAAAGTA+AGG | 0.390843 | 3.1:+79987790 | MS.gene48435:CDS |
| CAGATTAACACAAACCTGTT+TGG | 0.398433 | 3.1:-79988123 | None:intergenic |
| TGAGAGACAACCTCACCCTT+TGG | 0.419313 | 3.1:+79989607 | MS.gene48435:CDS |
| TATTTGGCTGAGTTTAAGAT+TGG | 0.435348 | 3.1:+79987848 | MS.gene48435:CDS |
| ATTGCTGAACTGGACACATT+AGG | 0.448432 | 3.1:+79989543 | MS.gene48435:CDS |
| TCAACTCATCCTATAAGATT+GGG | 0.451846 | 3.1:+79988032 | MS.gene48435:CDS |
| TGGTAGTATTCTGGAGTTGT+TGG | 0.453677 | 3.1:+79987748 | MS.gene48435:CDS |
| AGATGAAGGGTGATTATCAT+AGG | 0.459315 | 3.1:+79987825 | MS.gene48435:CDS |
| TCTTGTAAGCGACGGATATG+AGG | 0.463010 | 3.1:-79987586 | None:intergenic |
| CGTTTCTTCGATTGAACAGA+AGG | 0.478904 | 3.1:+79987643 | MS.gene48435:CDS |
| CCTTTGGACTTCTGATGTCC+AGG | 0.486316 | 3.1:+79989623 | MS.gene48435:CDS |
| GTTGTCTTACAAAGCTGCAC+AGG | 0.491542 | 3.1:+79987904 | MS.gene48435:CDS |
| TTCATGCAGAAGCTTGTAGT+GGG | 0.493249 | 3.1:+79987524 | MS.gene48435:CDS |
| AATCTTATAGGATGAGTTGA+TGG | 0.494756 | 3.1:-79988029 | None:intergenic |
| TCAGAGTTAACGAATGTTTG+TGG | 0.495537 | 3.1:+79987728 | MS.gene48435:CDS |
| TCGATTGAACAGAAGGAAGA+AGG | 0.502247 | 3.1:+79987650 | MS.gene48435:CDS |
| GATATCAAGGCTCATCTAGC+TGG | 0.506112 | 3.1:-79989788 | None:intergenic |
| CTTCATGCAGAAGCTTGTAG+TGG | 0.529618 | 3.1:+79987523 | MS.gene48435:CDS |
| CTTTCACTTGAAGAAGCAGA+AGG | 0.537834 | 3.1:-79987785 | None:intergenic |
| CACTACAAGCTTCTGCATGA+AGG | 0.552358 | 3.1:-79987522 | None:intergenic |
| GTCGCTTACAAGAACGTGAT+CGG | 0.573437 | 3.1:+79987596 | MS.gene48435:CDS |
| CAGAGCTTAGCGTCGAAGAG+AGG | 0.573495 | 3.1:+79987561 | MS.gene48435:CDS |
| CTGGACATCAGAAGTCCAAA+GGG | 0.585147 | 3.1:-79989622 | None:intergenic |
| AGCTGAACGCTACGAGGAGA+TGG | 0.585286 | 3.1:+79987496 | MS.gene48435:CDS |
| TCGACGCTAAGCTCTGATGA+TGG | 0.586328 | 3.1:-79987554 | None:intergenic |
| CATCAGAAGTCCAAAGGGTG+AGG | 0.595169 | 3.1:-79989617 | None:intergenic |
| ATCTGACAAAGCTTGTGACA+TGG | 0.613255 | 3.1:+79988100 | MS.gene48435:CDS |
| CCTGGACATCAGAAGTCCAA+AGG | 0.616148 | 3.1:-79989623 | None:intergenic |
| AGATCATGTTGTGCTTGTGA+AGG | 0.627894 | 3.1:+79987685 | MS.gene48435:CDS |
| GTTTAAGGATATTGCTGCTG+CGG | 0.646957 | 3.1:+79988001 | MS.gene48435:intron |
| GCTAAGCTCTGATGATGGTG+TGG | 0.653682 | 3.1:-79987549 | None:intergenic |
| TCATGCAGAAGCTTGTAGTG+GGG | 0.666058 | 3.1:+79987525 | MS.gene48435:CDS |
| AGAGAAGAGAGAATCATGGG+TGG | 0.672151 | 3.1:+79987413 | None:intergenic |
| TCGGATCGCTACGTGCTGCG+TGG | 0.678712 | 3.1:+79987615 | MS.gene48435:CDS |
| AGAACAAGCTGAACGCTACG+AGG | 0.693981 | 3.1:+79987490 | MS.gene48435:CDS |
| GATCACGTTCTTGTAAGCGA+CGG | 0.709037 | 3.1:-79987594 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAATAAATCACAAATTAA+AGG | - | chr3.1:79988288-79988307 | None:intergenic | 10.0% |
| !! | TCAAAAAATTTCATAGTTAT+TGG | + | chr3.1:79988620-79988639 | MS.gene48435:intron | 15.0% |
| !!! | GTTTTTTATTATAAGATGAA+GGG | + | chr3.1:79987812-79987831 | MS.gene48435:CDS | 15.0% |
| !!! | TATGATTTATATTTTAAGCT+CGG | - | chr3.1:79988721-79988740 | None:intergenic | 15.0% |
| !! | AGCATTATAAAATTAAACAG+AGG | - | chr3.1:79989381-79989400 | None:intergenic | 20.0% |
| !! | ATTATAAAATTAAACAGAGG+GGG | - | chr3.1:79989378-79989397 | None:intergenic | 20.0% |
| !! | CATTATAAAATTAAACAGAG+GGG | - | chr3.1:79989379-79989398 | None:intergenic | 20.0% |
| !! | GAGCTTAAAATATAAATCAT+AGG | + | chr3.1:79988720-79988739 | MS.gene48435:intron | 20.0% |
| !! | GCATTATAAAATTAAACAGA+GGG | - | chr3.1:79989380-79989399 | None:intergenic | 20.0% |
| !! | TGAGGTTATTATAATCTAAT+GGG | + | chr3.1:79988417-79988436 | MS.gene48435:intron | 20.0% |
| !! | TGCATTAAAGAAATCTATAT+TGG | + | chr3.1:79988994-79989013 | MS.gene48435:intron | 20.0% |
| !!! | AAACAATTTTCTTTTCCTTT+TGG | + | chr3.1:79989501-79989520 | MS.gene48435:intron | 20.0% |
| !!! | AACTATGAAATTTTTTGACT+AGG | - | chr3.1:79988618-79988637 | None:intergenic | 20.0% |
| !!! | AAGAGTGTATATCTATTTTA+CGG | - | chr3.1:79988821-79988840 | None:intergenic | 20.0% |
| !!! | AATATTGTTATTCATTTGCA+AGG | + | chr3.1:79989156-79989175 | MS.gene48435:intron | 20.0% |
| !!! | AATTCTCTTTTTTTTCTGTT+TGG | + | chr3.1:79989127-79989146 | MS.gene48435:intron | 20.0% |
| !!! | GCATTTTGATTAATAATCAT+TGG | + | chr3.1:79989698-79989717 | MS.gene48435:intron | 20.0% |
| !!! | GGTTTTTTATTATAAGATGA+AGG | + | chr3.1:79987811-79987830 | MS.gene48435:CDS | 20.0% |
| !!! | TATGTTAAAAACATTTTACC+TGG | - | chr3.1:79989644-79989663 | None:intergenic | 20.0% |
| !!! | TTTTAAAAAAGGTTTGTGTT+TGG | + | chr3.1:79988959-79988978 | MS.gene48435:intron | 20.0% |
| ! | AACTCAGATTATGATCAATT+TGG | - | chr3.1:79989744-79989763 | None:intergenic | 25.0% |
| ! | AACTGCAAATATATGGATAT+TGG | + | chr3.1:79988896-79988915 | MS.gene48435:intron | 25.0% |
| ! | AATCTGAGTTAAGCTATATT+TGG | + | chr3.1:79989754-79989773 | MS.gene48435:intron | 25.0% |
| ! | AATTCTAACTGCAGAAAATT+TGG | + | chr3.1:79988793-79988812 | MS.gene48435:intron | 25.0% |
| ! | AATTTCATAGTTATTGGAGT+TGG | + | chr3.1:79988626-79988645 | MS.gene48435:intron | 25.0% |
| ! | CTGAGGTTATTATAATCTAA+TGG | + | chr3.1:79988416-79988435 | MS.gene48435:intron | 25.0% |
| ! | TTATTCATCTGCAACAAATT+TGG | + | chr3.1:79989343-79989362 | MS.gene48435:intron | 25.0% |
| ! | TTTCATGGTTAAGTTATTGT+GGG | + | chr3.1:79988922-79988941 | MS.gene48435:intron | 25.0% |
| !! | ACAAATTAAAGGAAGCATAT+AGG | - | chr3.1:79988277-79988296 | None:intergenic | 25.0% |
| !! | ACTTGCTAGAGAAAAAAAAA+AGG | + | chr3.1:79989319-79989338 | MS.gene48435:intron | 25.0% |
| !! | ATTGTTGTGTTTTGTGTTTA+AGG | + | chr3.1:79987986-79988005 | MS.gene48435:intron | 25.0% |
| !! | ATTTTCTTTTCCTTTTGGAT+AGG | + | chr3.1:79989506-79989525 | MS.gene48435:intron | 25.0% |
| !! | TATACACTCTTTCTTTTACA+TGG | + | chr3.1:79988830-79988849 | MS.gene48435:intron | 25.0% |
| !!! | ACAGAAAAATGTTCTGAAAA+GGG | - | chr3.1:79989674-79989693 | None:intergenic | 25.0% |
| !!! | ATCTTGGTTTTTTGTTTGTT+TGG | + | chr3.1:79988146-79988165 | MS.gene48435:intron | 25.0% |
| !!! | ATGAAATTTTTTGACTAGGA+GGG | - | chr3.1:79988614-79988633 | None:intergenic | 25.0% |
| !!! | TATGAAATTTTTTGACTAGG+AGG | - | chr3.1:79988615-79988634 | None:intergenic | 25.0% |
| AAAGAAAAGAGAAACACAGA+AGG | - | chr3.1:79989462-79989481 | None:intergenic | 30.0% | |
| AAATTCAACACTAATTGCTG+AGG | + | chr3.1:79988399-79988418 | MS.gene48435:intron | 30.0% | |
| AAGAAATTCACAGCATATCT+AGG | - | chr3.1:79988570-79988589 | None:intergenic | 30.0% | |
| AGAATTCTCTCTTTACTCTT+TGG | + | chr3.1:79989244-79989263 | MS.gene48435:intron | 30.0% | |
| AGTGAATTTAACTCAGAATG+GGG | + | chr3.1:79989062-79989081 | MS.gene48435:intron | 30.0% | |
| ATCAACTCATCCTATAAGAT+TGG | + | chr3.1:79988031-79988050 | MS.gene48435:CDS | 30.0% | |
| ATTTGCAGTTAGACTACTAT+GGG | - | chr3.1:79988886-79988905 | None:intergenic | 30.0% | |
| CATCCTTTCATAAGATACAT+AGG | - | chr3.1:79988530-79988549 | None:intergenic | 30.0% | |
| GAGTGAATTTAACTCAGAAT+GGG | + | chr3.1:79989061-79989080 | MS.gene48435:intron | 30.0% | |
| GTAGTCTAACTGCAAATATA+TGG | + | chr3.1:79988889-79988908 | MS.gene48435:intron | 30.0% | |
| GTTTCATGGTTAAGTTATTG+TGG | + | chr3.1:79988921-79988940 | MS.gene48435:intron | 30.0% | |
| TAAAATTGCTTGACCAAACA+AGG | - | chr3.1:79988353-79988372 | None:intergenic | 30.0% | |
| TATTTGCAGTTAGACTACTA+TGG | - | chr3.1:79988887-79988906 | None:intergenic | 30.0% | |
| TCAACATAACAGAATCTTTG+TGG | - | chr3.1:79988378-79988397 | None:intergenic | 30.0% | |
| TCAACTCATCCTATAAGATT+GGG | + | chr3.1:79988032-79988051 | MS.gene48435:CDS | 30.0% | |
| TGAGTGAATTTAACTCAGAA+TGG | + | chr3.1:79989060-79989079 | MS.gene48435:intron | 30.0% | |
| TTCATGGTTAAGTTATTGTG+GGG | + | chr3.1:79988923-79988942 | MS.gene48435:intron | 30.0% | |
| ! | AATCTTATAGGATGAGTTGA+TGG | - | chr3.1:79988032-79988051 | None:intergenic | 30.0% |
| ! | GATCAATTTGGAGCAATATT+AGG | - | chr3.1:79989732-79989751 | None:intergenic | 30.0% |
| ! | TATGGATATTGGTAGTTTCA+TGG | + | chr3.1:79988907-79988926 | MS.gene48435:intron | 30.0% |
| ! | TATTTGGCTGAGTTTAAGAT+TGG | + | chr3.1:79987848-79987867 | MS.gene48435:CDS | 30.0% |
| !! | GACAGAAAAATGTTCTGAAA+AGG | - | chr3.1:79989675-79989694 | None:intergenic | 30.0% |
| ACACATACTGTTCTCTGTTT+AGG | - | chr3.1:79987454-79987473 | None:intergenic | 35.0% | |
| AGATGAAGGGTGATTATCAT+AGG | + | chr3.1:79987825-79987844 | MS.gene48435:CDS | 35.0% | |
| ATTAAACAGAGGGGGAAAAA+AGG | - | chr3.1:79989370-79989389 | None:intergenic | 35.0% | |
| CAGATTAACACAAACCTGTT+TGG | - | chr3.1:79988126-79988145 | None:intergenic | 35.0% | |
| CGTTAACTCTGATTCAACTT+TGG | - | chr3.1:79987720-79987739 | None:intergenic | 35.0% | |
| GAATGAGCAATTGACATGTT+TGG | + | chr3.1:79988458-79988477 | MS.gene48435:intron | 35.0% | |
| GAATGTTTGTGGTAGTATTC+TGG | + | chr3.1:79987739-79987758 | MS.gene48435:CDS | 35.0% | |
| GGGTGATTATCATAGGTATT+TGG | + | chr3.1:79987832-79987851 | MS.gene48435:CDS | 35.0% | |
| GGTTTGTGTTAATCTGATCT+TGG | + | chr3.1:79988130-79988149 | MS.gene48435:intron | 35.0% | |
| GTGCCTATGTATCTTATGAA+AGG | + | chr3.1:79988524-79988543 | MS.gene48435:intron | 35.0% | |
| TATAAGTGTGCTGTCTTTGT+AGG | - | chr3.1:79989577-79989596 | None:intergenic | 35.0% | |
| TATGGGTTCATTGTTCATTC+AGG | - | chr3.1:79988869-79988888 | None:intergenic | 35.0% | |
| TCAGAGTTAACGAATGTTTG+TGG | + | chr3.1:79987728-79987747 | MS.gene48435:CDS | 35.0% | |
| TCATCCTATAAGATTGGGTT+TGG | + | chr3.1:79988037-79988056 | MS.gene48435:CDS | 35.0% | |
| TGCTTCTTCAAGTGAAAGTA+AGG | + | chr3.1:79987790-79987809 | MS.gene48435:CDS | 35.0% | |
| TGTCAATTGCTCATTCTTAG+TGG | - | chr3.1:79988454-79988473 | None:intergenic | 35.0% | |
| TTCTGATTTACACAGTGTGT+TGG | + | chr3.1:79988758-79988777 | MS.gene48435:intron | 35.0% | |
| ! | AAAAGTTTGCTCATGAAGCT+GGG | + | chr3.1:79989021-79989040 | MS.gene48435:intron | 35.0% |
| ! | AAGCTATATTTGGCTTATGC+AGG | + | chr3.1:79989764-79989783 | MS.gene48435:intron | 35.0% |
| ! | ACTTGATAACACAACCAGTA+TGG | - | chr3.1:79989419-79989438 | None:intergenic | 35.0% |
| ! | AGAAGTTTTCTGACCTTGTT+TGG | + | chr3.1:79988337-79988356 | MS.gene48435:intron | 35.0% |
| ! | GTAAGAAATCCGTTTGACTT+TGG | + | chr3.1:79988496-79988515 | MS.gene48435:intron | 35.0% |
| ! | TAAAAGTTTGCTCATGAAGC+TGG | + | chr3.1:79989020-79989039 | MS.gene48435:intron | 35.0% |
| !! | AAGAGTGTTGTTTACCATAC+TGG | + | chr3.1:79989402-79989421 | MS.gene48435:intron | 35.0% |
| !! | GAAAAATGTTCTGAAAAGGG+TGG | - | chr3.1:79989671-79989690 | None:intergenic | 35.0% |
| !! | TGTTCTCTGTTTAGGTTTTC+TGG | - | chr3.1:79987446-79987465 | None:intergenic | 35.0% |
| AGAGCCAAACCCAATCTTAT+AGG | - | chr3.1:79988044-79988063 | None:intergenic | 40.0% | |
| AGATCATGTTGTGCTTGTGA+AGG | + | chr3.1:79987685-79987704 | MS.gene48435:CDS | 40.0% | |
| ATCTGACAAAGCTTGTGACA+TGG | + | chr3.1:79988100-79988119 | MS.gene48435:CDS | 40.0% | |
| ATGAAAGGATGTGTGAAAGC+TGG | + | chr3.1:79988539-79988558 | MS.gene48435:intron | 40.0% | |
| ATTGCTGAACTGGACACATT+AGG | + | chr3.1:79989543-79989562 | MS.gene48435:CDS | 40.0% | |
| CGTTTCTTCGATTGAACAGA+AGG | + | chr3.1:79987643-79987662 | MS.gene48435:CDS | 40.0% | |
| CTTTCACTTGAAGAAGCAGA+AGG | - | chr3.1:79987788-79987807 | None:intergenic | 40.0% | |
| GCACAAACACCAAAGTCAAA+CGG | - | chr3.1:79988508-79988527 | None:intergenic | 40.0% | |
| GTTTAAGGATATTGCTGCTG+CGG | + | chr3.1:79988001-79988020 | MS.gene48435:intron | 40.0% | |
| TCCTCAAAAGCCTATCCAAA+AGG | - | chr3.1:79989519-79989538 | None:intergenic | 40.0% | |
| TCGATTGAACAGAAGGAAGA+AGG | + | chr3.1:79987650-79987669 | MS.gene48435:CDS | 40.0% | |
| TGAGAAAAAATCTGCTGCTG+AGG | + | chr3.1:79987874-79987893 | MS.gene48435:CDS | 40.0% | |
| TGGAGCAATATTAGGAAGAG+AGG | - | chr3.1:79989724-79989743 | None:intergenic | 40.0% | |
| TTCATGCAGAAGCTTGTAGT+GGG | + | chr3.1:79987524-79987543 | MS.gene48435:CDS | 40.0% | |
| ! | CTCTTTCTTTTACATGGCTG+TGG | + | chr3.1:79988836-79988855 | MS.gene48435:intron | 40.0% |
| ! | TCCTTTTGGATAGGCTTTTG+AGG | + | chr3.1:79989515-79989534 | MS.gene48435:intron | 40.0% |
| !! | TGGTAGTATTCTGGAGTTGT+TGG | + | chr3.1:79987748-79987767 | MS.gene48435:CDS | 40.0% |
| ATGGGGAAATTGCACATGGA+AGG | + | chr3.1:79989079-79989098 | MS.gene48435:intron | 45.0% | |
| CACTACAAGCTTCTGCATGA+AGG | - | chr3.1:79987525-79987544 | None:intergenic | 45.0% | |
| CAGAATGGGGAAATTGCACA+TGG | + | chr3.1:79989075-79989094 | MS.gene48435:intron | 45.0% | |
| CTGGACATCAGAAGTCCAAA+GGG | - | chr3.1:79989625-79989644 | None:intergenic | 45.0% | |
| CTTCATGCAGAAGCTTGTAG+TGG | + | chr3.1:79987523-79987542 | MS.gene48435:CDS | 45.0% | |
| GATCACGTTCTTGTAAGCGA+CGG | - | chr3.1:79987597-79987616 | None:intergenic | 45.0% | |
| GTCGCTTACAAGAACGTGAT+CGG | + | chr3.1:79987596-79987615 | MS.gene48435:CDS | 45.0% | |
| GTTGTCTTACAAAGCTGCAC+AGG | + | chr3.1:79987904-79987923 | MS.gene48435:CDS | 45.0% | |
| TCATGCAGAAGCTTGTAGTG+GGG | + | chr3.1:79987525-79987544 | MS.gene48435:CDS | 45.0% | |
| TCTTGTAAGCGACGGATATG+AGG | - | chr3.1:79987589-79987608 | None:intergenic | 45.0% | |
| TGAGGAAGCAATTGCTGAAC+TGG | + | chr3.1:79989533-79989552 | MS.gene48435:CDS | 45.0% | |
| ! | CTTGTTCTGCGAGTTTTGCT+AGG | - | chr3.1:79987478-79987497 | None:intergenic | 45.0% |
| !!! | AACTTATAATTTTTTAAAAA+AGG | + | chr3.1:79988948-79988967 | MS.gene48435:intron | 5.0% |
| !!! | TTTATTATATTGAAAATTTT+AGG | + | chr3.1:79988303-79988322 | MS.gene48435:intron | 5.0% |
| AGAACAAGCTGAACGCTACG+AGG | + | chr3.1:79987490-79987509 | MS.gene48435:CDS | 50.0% | |
| AGCTTGTGACATGGCCAAAC+AGG | + | chr3.1:79988109-79988128 | MS.gene48435:CDS | 50.0% | |
| CATCAGAAGTCCAAAGGGTG+AGG | - | chr3.1:79989620-79989639 | None:intergenic | 50.0% | |
| CCTGGACATCAGAAGTCCAA+AGG | - | chr3.1:79989626-79989645 | None:intergenic | 50.0% | |
| CCTTTGGACTTCTGATGTCC+AGG | + | chr3.1:79989623-79989642 | MS.gene48435:CDS | 50.0% | |
| TGAGAGACAACCTCACCCTT+TGG | + | chr3.1:79989607-79989626 | MS.gene48435:CDS | 50.0% | |
| ! | TCGACGCTAAGCTCTGATGA+TGG | - | chr3.1:79987557-79987576 | None:intergenic | 50.0% |
| !! | GCTAAGCTCTGATGATGGTG+TGG | - | chr3.1:79987552-79987571 | None:intergenic | 50.0% |
| AGCTGAACGCTACGAGGAGA+TGG | + | chr3.1:79987496-79987515 | MS.gene48435:CDS | 55.0% | |
| ! | CAGAGCTTAGCGTCGAAGAG+AGG | + | chr3.1:79987561-79987580 | MS.gene48435:CDS | 55.0% |
| TCGGATCGCTACGTGCTGCG+TGG | + | chr3.1:79987615-79987634 | MS.gene48435:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 79987428 | 79989806 | 79987428 | ID=MS.gene48435 |
| chr3.1 | mRNA | 79987428 | 79989806 | 79987428 | ID=MS.gene48435.t1;Parent=MS.gene48435 |
| chr3.1 | exon | 79987428 | 79987925 | 79987428 | ID=MS.gene48435.t1.exon1;Parent=MS.gene48435.t1 |
| chr3.1 | CDS | 79987428 | 79987925 | 79987428 | ID=cds.MS.gene48435.t1;Parent=MS.gene48435.t1 |
| chr3.1 | exon | 79988008 | 79988130 | 79988008 | ID=MS.gene48435.t1.exon2;Parent=MS.gene48435.t1 |
| chr3.1 | CDS | 79988008 | 79988130 | 79988008 | ID=cds.MS.gene48435.t1;Parent=MS.gene48435.t1 |
| chr3.1 | exon | 79989528 | 79989644 | 79989528 | ID=MS.gene48435.t1.exon3;Parent=MS.gene48435.t1 |
| chr3.1 | CDS | 79989528 | 79989644 | 79989528 | ID=cds.MS.gene48435.t1;Parent=MS.gene48435.t1 |
| chr3.1 | exon | 79989786 | 79989806 | 79989786 | ID=MS.gene48435.t1.exon4;Parent=MS.gene48435.t1 |
| chr3.1 | CDS | 79989786 | 79989806 | 79989786 | ID=cds.MS.gene48435.t1;Parent=MS.gene48435.t1 |
| Gene Sequence |
| Protein sequence |
