Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055488.t1 | XP_003602823.2 | 100 | 260 | 0 | 0 | 1 | 260 | 1 | 260 | 1.50E-137 | 498.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055488.t1 | P46266 | 96.9 | 260 | 8 | 0 | 1 | 260 | 1 | 260 | 2.4e-136 | 486.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055488.t1 | G7J3X9 | 100.0 | 260 | 0 | 0 | 1 | 260 | 1 | 260 | 1.1e-137 | 498.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050640 | MS.gene055488 | -0.840881 | 6.66E-58 | -1.69E-46 |
| MS.gene051208 | MS.gene055488 | -0.816772 | 4.66E-52 | -1.69E-46 |
| MS.gene052011 | MS.gene055488 | 0.825168 | 5.43E-54 | -1.69E-46 |
| MS.gene055217 | MS.gene055488 | -0.808316 | 3.29E-50 | -1.69E-46 |
| MS.gene055488 | MS.gene056297 | -0.811015 | 8.65E-51 | -1.69E-46 |
| MS.gene055488 | MS.gene056442 | -0.822527 | 2.26E-53 | -1.69E-46 |
| MS.gene055488 | MS.gene059683 | -0.80589 | 1.07E-49 | -1.69E-46 |
| MS.gene055488 | MS.gene061114 | 0.801638 | 8.19E-49 | -1.69E-46 |
| MS.gene055488 | MS.gene064688 | -0.823105 | 1.66E-53 | -1.69E-46 |
| MS.gene055488 | MS.gene065011 | -0.810633 | 1.05E-50 | -1.69E-46 |
| MS.gene055488 | MS.gene065350 | 0.813406 | 2.60E-51 | -1.69E-46 |
| MS.gene055488 | MS.gene071517 | -0.801928 | 7.14E-49 | -1.69E-46 |
| MS.gene055488 | MS.gene071518 | -0.805989 | 1.02E-49 | -1.69E-46 |
| MS.gene055488 | MS.gene071519 | -0.801775 | 7.68E-49 | -1.69E-46 |
| MS.gene055488 | MS.gene071520 | -0.837977 | 3.78E-57 | -1.69E-46 |
| MS.gene055488 | MS.gene09667 | -0.806598 | 7.61E-50 | -1.69E-46 |
| MS.gene055488 | MS.gene21480 | -0.803165 | 3.97E-49 | -1.69E-46 |
| MS.gene055488 | MS.gene22517 | -0.814541 | 1.46E-51 | -1.69E-46 |
| MS.gene055488 | MS.gene22803 | -0.840701 | 7.42E-58 | -1.69E-46 |
| MS.gene055488 | MS.gene28385 | -0.803426 | 3.51E-49 | -1.69E-46 |
| MS.gene055488 | MS.gene38033 | -0.821611 | 3.68E-53 | -1.69E-46 |
| MS.gene055488 | MS.gene38223 | -0.815395 | 9.45E-52 | -1.69E-46 |
| MS.gene055488 | MS.gene38593 | 0.93072 | 1.09E-93 | -1.69E-46 |
| MS.gene055488 | MS.gene42319 | -0.81089 | 9.20E-51 | -1.69E-46 |
| MS.gene055488 | MS.gene42798 | -0.812049 | 5.16E-51 | -1.69E-46 |
| MS.gene055488 | MS.gene49850 | -0.814821 | 1.27E-51 | -1.69E-46 |
| MS.gene055488 | MS.gene54663 | -0.817855 | 2.66E-52 | -1.69E-46 |
| MS.gene055488 | MS.gene56071 | -0.815582 | 8.59E-52 | -1.69E-46 |
| MS.gene055488 | MS.gene56072 | -0.811989 | 5.31E-51 | -1.69E-46 |
| MS.gene055488 | MS.gene56074 | -0.80738 | 5.20E-50 | -1.69E-46 |
| MS.gene055488 | MS.gene56403 | -0.801962 | 7.03E-49 | -1.69E-46 |
| MS.gene055488 | MS.gene57835 | 0.803476 | 3.42E-49 | -1.69E-46 |
| MS.gene055488 | MS.gene61547 | 0.806132 | 9.54E-50 | -1.69E-46 |
| MS.gene055488 | MS.gene81245 | -0.816326 | 5.86E-52 | -1.69E-46 |
| MS.gene055488 | MS.gene85276 | 0.818117 | 2.32E-52 | -1.69E-46 |
| MS.gene055488 | MS.gene86113 | -0.823943 | 1.06E-53 | -1.69E-46 |
| MS.gene055488 | MS.gene86351 | 0.80257 | 5.27E-49 | -1.69E-46 |
| MS.gene055488 | MS.gene89004 | -0.822017 | 2.97E-53 | -1.69E-46 |
| MS.gene055488 | MS.gene89337 | -0.812989 | 3.21E-51 | -1.69E-46 |
| MS.gene055488 | MS.gene91562 | -0.809689 | 1.67E-50 | -1.69E-46 |
| MS.gene055488 | MS.gene92106 | -0.830791 | 2.41E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene067412 | MS.gene055488 | PPI |
| MS.gene055488 | MS.gene050079 | PPI |
| MS.gene055488 | MS.gene047668 | PPI |
| MS.gene055488 | MS.gene38593 | PPI |
| MS.gene56475 | MS.gene055488 | PPI |
| MS.gene055488 | MS.gene53188 | PPI |
| MS.gene055488 | MS.gene043907 | PPI |
| MS.gene055488 | MS.gene010393 | PPI |
| MS.gene041125 | MS.gene055488 | PPI |
| MS.gene055488 | MS.gene55401 | PPI |
| MS.gene055488 | MS.gene010179 | PPI |
| MS.gene48435 | MS.gene055488 | PPI |
| MS.gene55400 | MS.gene055488 | PPI |
| MS.gene055488 | MS.gene010180 | PPI |
| MS.gene020411 | MS.gene055488 | PPI |
| MS.gene71392 | MS.gene055488 | PPI |
| MS.gene013133 | MS.gene055488 | PPI |
| MS.gene006850 | MS.gene055488 | PPI |
| MS.gene055488 | MS.gene035837 | PPI |
| MS.gene055488 | MS.gene052176 | PPI |
| MS.gene055488 | MS.gene31370 | PPI |
| MS.gene055488 | MS.gene054662 | PPI |
| MS.gene066779 | MS.gene055488 | PPI |
| MS.gene055488 | MS.gene028807 | PPI |
| MS.gene055488 | MS.gene83541 | PPI |
| MS.gene043514 | MS.gene055488 | PPI |
| MS.gene055488 | MS.gene86409 | PPI |
| MS.gene055488 | MS.gene013047 | PPI |
| MS.gene54847 | MS.gene055488 | PPI |
| MS.gene055488 | MS.gene64150 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055488.t1 | MTR_3g099380 | 100.000 | 260 | 0 | 0 | 1 | 260 | 1 | 260 | 0.0 | 533 |
| MS.gene055488.t1 | MTR_5g064580 | 90.769 | 260 | 24 | 0 | 1 | 260 | 1 | 260 | 4.87e-177 | 488 |
| MS.gene055488.t1 | MTR_3g099380 | 100.000 | 203 | 0 | 0 | 1 | 203 | 1 | 203 | 6.31e-150 | 417 |
| MS.gene055488.t1 | MTR_3g100620 | 76.793 | 237 | 54 | 1 | 8 | 243 | 11 | 247 | 1.50e-135 | 382 |
| MS.gene055488.t1 | MTR_2g091225 | 72.840 | 243 | 64 | 2 | 3 | 245 | 2 | 242 | 7.14e-129 | 365 |
| MS.gene055488.t1 | MTR_4g083060 | 70.518 | 251 | 72 | 2 | 8 | 258 | 7 | 255 | 1.48e-126 | 361 |
| MS.gene055488.t1 | MTR_5g044160 | 71.951 | 246 | 67 | 2 | 3 | 248 | 2 | 245 | 1.62e-126 | 360 |
| MS.gene055488.t1 | MTR_8g086270 | 71.371 | 248 | 69 | 2 | 3 | 250 | 2 | 247 | 1.63e-125 | 357 |
| MS.gene055488.t1 | MTR_2g015980 | 69.444 | 252 | 73 | 3 | 6 | 256 | 3 | 251 | 4.17e-124 | 353 |
| MS.gene055488.t1 | MTR_2g076960 | 66.275 | 255 | 84 | 1 | 1 | 255 | 1 | 253 | 8.05e-122 | 348 |
| MS.gene055488.t1 | MTR_2g091225 | 72.727 | 220 | 58 | 2 | 26 | 245 | 2 | 219 | 2.99e-117 | 335 |
| MS.gene055488.t1 | MTR_0137s0020 | 59.244 | 238 | 91 | 4 | 8 | 243 | 11 | 244 | 6.46e-91 | 269 |
| MS.gene055488.t1 | MTR_3g014060 | 58.824 | 238 | 92 | 4 | 8 | 243 | 11 | 244 | 5.08e-90 | 267 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055488.t1 | AT1G78300 | 91.797 | 256 | 20 | 1 | 6 | 260 | 3 | 258 | 6.41e-173 | 477 |
| MS.gene055488.t1 | AT1G35160 | 87.259 | 259 | 32 | 1 | 3 | 260 | 6 | 264 | 1.61e-167 | 464 |
| MS.gene055488.t1 | AT4G09000 | 86.260 | 262 | 32 | 1 | 3 | 260 | 5 | 266 | 1.57e-164 | 456 |
| MS.gene055488.t1 | AT1G35160 | 78.746 | 287 | 32 | 2 | 3 | 260 | 6 | 292 | 2.11e-161 | 449 |
| MS.gene055488.t1 | AT5G38480 | 86.111 | 252 | 34 | 1 | 6 | 257 | 2 | 252 | 1.13e-160 | 446 |
| MS.gene055488.t1 | AT3G02520 | 84.706 | 255 | 37 | 2 | 6 | 258 | 3 | 257 | 8.61e-159 | 441 |
| MS.gene055488.t1 | AT3G02520 | 84.706 | 255 | 37 | 2 | 6 | 258 | 3 | 257 | 8.61e-159 | 441 |
| MS.gene055488.t1 | AT5G38480 | 86.111 | 252 | 33 | 2 | 6 | 257 | 2 | 251 | 2.41e-158 | 440 |
| MS.gene055488.t1 | AT5G38480 | 86.111 | 252 | 33 | 2 | 6 | 257 | 2 | 251 | 2.41e-158 | 440 |
| MS.gene055488.t1 | AT4G09000 | 88.750 | 240 | 27 | 0 | 3 | 242 | 5 | 244 | 3.67e-158 | 442 |
| MS.gene055488.t1 | AT5G16050 | 82.308 | 260 | 43 | 1 | 3 | 259 | 2 | 261 | 5.38e-150 | 419 |
| MS.gene055488.t1 | AT5G16050 | 82.308 | 260 | 43 | 1 | 3 | 259 | 2 | 261 | 5.38e-150 | 419 |
| MS.gene055488.t1 | AT5G10450 | 78.776 | 245 | 51 | 1 | 3 | 246 | 2 | 246 | 4.34e-143 | 401 |
| MS.gene055488.t1 | AT5G10450 | 76.800 | 250 | 57 | 1 | 3 | 251 | 2 | 251 | 1.20e-142 | 401 |
| MS.gene055488.t1 | AT5G10450 | 78.926 | 242 | 50 | 1 | 3 | 243 | 2 | 243 | 1.51e-142 | 400 |
| MS.gene055488.t1 | AT5G10450 | 79.253 | 241 | 49 | 1 | 3 | 242 | 2 | 242 | 6.29e-142 | 399 |
| MS.gene055488.t1 | AT5G65430 | 79.167 | 240 | 49 | 1 | 8 | 246 | 7 | 246 | 6.64e-141 | 395 |
| MS.gene055488.t1 | AT5G65430 | 79.325 | 237 | 48 | 1 | 8 | 243 | 7 | 243 | 1.68e-140 | 395 |
| MS.gene055488.t1 | AT5G65430 | 79.661 | 236 | 47 | 1 | 8 | 242 | 7 | 242 | 5.97e-140 | 394 |
| MS.gene055488.t1 | AT1G26480 | 72.222 | 252 | 67 | 2 | 8 | 258 | 10 | 259 | 1.16e-129 | 368 |
| MS.gene055488.t1 | AT1G34760 | 71.429 | 238 | 66 | 1 | 8 | 245 | 5 | 240 | 3.38e-122 | 348 |
| MS.gene055488.t1 | AT1G34760 | 71.429 | 238 | 66 | 1 | 8 | 245 | 5 | 240 | 4.82e-122 | 348 |
| MS.gene055488.t1 | AT2G42590 | 70.539 | 241 | 69 | 1 | 8 | 248 | 7 | 245 | 1.26e-121 | 347 |
| MS.gene055488.t1 | AT2G42590 | 70.539 | 241 | 69 | 1 | 8 | 248 | 7 | 245 | 1.30e-121 | 348 |
| MS.gene055488.t1 | AT2G42590 | 69.200 | 250 | 74 | 2 | 8 | 256 | 7 | 254 | 3.98e-121 | 346 |
| MS.gene055488.t1 | AT1G22300 | 67.331 | 251 | 80 | 2 | 8 | 258 | 5 | 253 | 5.38e-116 | 333 |
| MS.gene055488.t1 | AT1G22300 | 67.331 | 251 | 80 | 2 | 8 | 258 | 5 | 253 | 6.55e-116 | 333 |
| MS.gene055488.t1 | AT1G22300 | 69.038 | 239 | 72 | 2 | 8 | 246 | 5 | 241 | 1.08e-114 | 329 |
| MS.gene055488.t1 | AT1G78220 | 48.069 | 233 | 118 | 2 | 8 | 239 | 5 | 235 | 1.19e-70 | 217 |
| MS.gene055488.t1 | AT1G22290 | 45.238 | 210 | 93 | 5 | 11 | 217 | 8 | 198 | 9.56e-48 | 158 |
| MS.gene055488.t1 | AT1G22290 | 44.928 | 207 | 92 | 5 | 11 | 214 | 8 | 195 | 1.92e-47 | 156 |
| MS.gene055488.t1 | AT2G10450 | 71.429 | 49 | 14 | 0 | 207 | 255 | 16 | 64 | 4.40e-18 | 77.0 |
Find 71 sgRNAs with CRISPR-Local
Find 128 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGCAAGACCCAGCCTAAT+TGG | 0.274942 | 3.2:-82679191 | None:intergenic |
| GACGGTGAGTTCTTCGTTAT+CGG | 0.280595 | 3.2:-82678485 | None:intergenic |
| CTCATTCCTTCCGCTGCTTC+CGG | 0.316176 | 3.2:+82678712 | MS.gene055488:CDS |
| TGATGAAGCAATTGCTGAAC+TGG | 0.341177 | 3.2:+82680049 | MS.gene055488:CDS |
| TACTGCTGTTCATCGGGTTT+AGG | 0.348488 | 3.2:-82680289 | None:intergenic |
| CGAAGAAATGGTGGAGTTCA+TGG | 0.350821 | 3.2:+82678444 | MS.gene055488:CDS |
| AACCTGTTTAGCAAGATTGC+AGG | 0.360144 | 3.2:-82679261 | None:intergenic |
| CACCATTTCTTCGTAGCGTT+CGG | 0.376229 | 3.2:-82678434 | None:intergenic |
| TGCTCCGATGACGTTCTTGT+AGG | 0.378815 | 3.2:-82678533 | None:intergenic |
| TTCGTAGCGTTCGGCTTGTT+CGG | 0.387733 | 3.2:-82678425 | None:intergenic |
| TGCCTGCAATCTTGCTAAAC+AGG | 0.391731 | 3.2:+82679259 | MS.gene055488:CDS |
| GGTAATCTCCCTTCATCTTA+AGG | 0.395416 | 3.2:-82678753 | None:intergenic |
| CGCTGCTTCCGGTGATTCCA+AGG | 0.396427 | 3.2:+82678723 | MS.gene055488:CDS |
| TTTGTTTGGTTTCAGGATGA+TGG | 0.406229 | 3.2:+82680241 | MS.gene055488:intron |
| AGCCGAACGCTACGAAGAAA+TGG | 0.413922 | 3.2:+82678432 | MS.gene055488:CDS |
| TTTGTTTACTGCTGTTCATC+GGG | 0.421726 | 3.2:-82680295 | None:intergenic |
| TGCCACCAACTCATCCAATT+AGG | 0.435904 | 3.2:+82679177 | MS.gene055488:CDS |
| GCAAGATTGCAGGCACGATC+AGG | 0.442951 | 3.2:-82679251 | None:intergenic |
| AGCTGCTTCTTTAATTTCAT+CGG | 0.448476 | 3.2:-82680266 | None:intergenic |
| TTCTTCGTTATCGGCGTTGG+CGG | 0.458530 | 3.2:-82678476 | None:intergenic |
| ACCAACTCATCCAATTAGGC+TGG | 0.462152 | 3.2:+82679181 | MS.gene055488:CDS |
| CTCAGCAGCCTCCTTACGCT+CGG | 0.471405 | 3.2:-82678806 | None:intergenic |
| CCTGCATGTCAGAGGTCCAT+AGG | 0.480592 | 3.2:-82680139 | None:intergenic |
| ATTTGTTTACTGCTGTTCAT+CGG | 0.482074 | 3.2:-82680296 | None:intergenic |
| TCGGAGCACGACGTGCTTCG+TGG | 0.495843 | 3.2:+82678548 | MS.gene055488:CDS |
| CCCAGCCTAATTGGATGAGT+TGG | 0.496782 | 3.2:-82679182 | None:intergenic |
| CGCCGCTTACAAATCTGCTC+AGG | 0.501140 | 3.2:+82678837 | MS.gene055488:CDS |
| CATCTTAAGGTAGAAAACCT+TGG | 0.502188 | 3.2:-82678740 | None:intergenic |
| GGCGGCCGCTCATTCACCTC+GGG | 0.502842 | 3.2:+82678375 | MS.gene055488:CDS |
| CAAGACTGGCGCCGAGCGTA+AGG | 0.509038 | 3.2:+82678795 | MS.gene055488:CDS |
| TTCCTCGATCGAACAGAAAG+AGG | 0.515919 | 3.2:+82678579 | MS.gene055488:CDS |
| GAGTTCTTCGTTATCGGCGT+TGG | 0.515957 | 3.2:-82678479 | None:intergenic |
| AGCCTAATTGGATGAGTTGG+TGG | 0.523887 | 3.2:-82679179 | None:intergenic |
| TTCGTGATAATCTCACCCTA+TGG | 0.525895 | 3.2:+82680123 | MS.gene055488:CDS |
| GTAGAAAACCTTGGAATCAC+CGG | 0.533118 | 3.2:-82678731 | None:intergenic |
| CCTATGGACCTCTGACATGC+AGG | 0.534557 | 3.2:+82680139 | MS.gene055488:CDS |
| AGATCGGTAGTCACGGATGA+CGG | 0.545474 | 3.2:-82678629 | None:intergenic |
| CTGCATGTCAGAGGTCCATA+GGG | 0.549120 | 3.2:-82680138 | None:intergenic |
| AGCAGCGGAAGGAATGAGAC+GGG | 0.550202 | 3.2:-82678707 | None:intergenic |
| TGAACTCAGCAAGATACCTA+TGG | 0.552878 | 3.2:-82678774 | None:intergenic |
| CTCCTCTTTCTGTTCGATCG+AGG | 0.555820 | 3.2:-82678581 | None:intergenic |
| AAGAACTCACCGTCGAAGAG+AGG | 0.556409 | 3.2:+82678494 | MS.gene055488:CDS |
| TATCTTGCTGAGTTCAAGAC+TGG | 0.559984 | 3.2:+82678781 | MS.gene055488:CDS |
| CTACCGATCTAAGATCGAAT+CGG | 0.562157 | 3.2:+82678642 | MS.gene055488:CDS |
| CCTTGGCGAGGAATCATACA+AGG | 0.572604 | 3.2:+82680076 | MS.gene055488:CDS |
| TCGGGAAGAGAACGTTTACA+TGG | 0.578574 | 3.2:+82678393 | MS.gene055488:CDS |
| GTAGCCTACAAGAACGTCAT+CGG | 0.580177 | 3.2:+82678529 | MS.gene055488:CDS |
| ATTGCTGAACTGGATACCCT+TGG | 0.584167 | 3.2:+82680059 | MS.gene055488:CDS |
| CTTGTATGATTCCTCGCCAA+GGG | 0.586259 | 3.2:-82680075 | None:intergenic |
| CCAACTCATCCAATTAGGCT+GGG | 0.587390 | 3.2:+82679182 | MS.gene055488:CDS |
| TGGCGGCCGCTCATTCACCT+CGG | 0.588265 | 3.2:+82678374 | MS.gene055488:CDS |
| CTTGGAATCACCGGAAGCAG+CGG | 0.589029 | 3.2:-82678722 | None:intergenic |
| AAGCAGCGGAAGGAATGAGA+CGG | 0.594104 | 3.2:-82678708 | None:intergenic |
| GACTGGCGCCGAGCGTAAGG+AGG | 0.597871 | 3.2:+82678798 | MS.gene055488:CDS |
| CCTTGTATGATTCCTCGCCA+AGG | 0.605796 | 3.2:-82680076 | None:intergenic |
| AAACGTGATCCTCGTTGCCG+CGG | 0.607870 | 3.2:-82678606 | None:intergenic |
| AGATGAAGGGAGATTACCAT+AGG | 0.607967 | 3.2:+82678758 | MS.gene055488:CDS |
| GAGAAGGTTCCTCTCTTCGA+CGG | 0.608781 | 3.2:-82678503 | None:intergenic |
| TCTTGTAGGCTACAGAGAGA+AGG | 0.628236 | 3.2:-82678519 | None:intergenic |
| GAATCACCGGAAGCAGCGGA+AGG | 0.629660 | 3.2:-82678718 | None:intergenic |
| AATCAATACCTGCATGTCAG+AGG | 0.637050 | 3.2:-82680147 | None:intergenic |
| ATGTAAACGTTCTCTTCCCG+AGG | 0.637688 | 3.2:-82678391 | None:intergenic |
| GCTCCGATTCGATCTTAGAT+CGG | 0.641337 | 3.2:-82678645 | None:intergenic |
| CGATCTTAGATCGGTAGTCA+CGG | 0.645752 | 3.2:-82678636 | None:intergenic |
| CTCTTCCCGAGGTGAATGAG+CGG | 0.645933 | 3.2:-82678380 | None:intergenic |
| AGAGGAGAGCCGCGGCAACG+AGG | 0.653525 | 3.2:+82678597 | MS.gene055488:CDS |
| GAGCTTTCTAACATCTGTGA+CGG | 0.658608 | 3.2:+82678664 | MS.gene055488:CDS |
| GAACAGAAAGAGGAGAGCCG+CGG | 0.660532 | 3.2:+82678589 | MS.gene055488:CDS |
| AACCTGAGCAGATTTGTAAG+CGG | 0.663795 | 3.2:-82678839 | None:intergenic |
| TGAACTGGATACCCTTGGCG+AGG | 0.679766 | 3.2:+82680064 | MS.gene055488:CDS |
| CGAACGCTACGAAGAAATGG+TGG | 0.680192 | 3.2:+82678435 | MS.gene055488:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGAAGAAAACAAAAATATA+TGG | - | chr3.2:82680197-82680216 | None:intergenic | 15.0% |
| !! | AGAAGAAAACAAAAATATAT+GGG | - | chr3.2:82680196-82680215 | None:intergenic | 15.0% |
| !! | ATCTTTGTATATGAAATATA+TGG | + | chr3.2:82679073-82679092 | MS.gene055488:intron | 15.0% |
| !! | CATTTCAAAATGAAATTTAA+AGG | - | chr3.2:82679917-82679936 | None:intergenic | 15.0% |
| !!! | GTTATTTTGTAGTTATTTTA+AGG | + | chr3.2:82679322-82679341 | MS.gene055488:intron | 15.0% |
| !! | AAACAACAAAAAGGAAAAAA+CGG | - | chr3.2:82678867-82678886 | None:intergenic | 20.0% |
| !! | AATAAGATGTATATCCAAAA+AGG | - | chr3.2:82679348-82679367 | None:intergenic | 20.0% |
| !! | AATAATAAGGATACCAAAAA+AGG | - | chr3.2:82679699-82679718 | None:intergenic | 20.0% |
| !! | ATTTAAAGGAATAAATTCCA+TGG | - | chr3.2:82679903-82679922 | None:intergenic | 20.0% |
| !! | TTTAAAGGAATAAATTCCAT+GGG | - | chr3.2:82679902-82679921 | None:intergenic | 20.0% |
| !!! | CTCATGAATATTTTTTTGTT+TGG | + | chr3.2:82680011-82680030 | MS.gene055488:intron | 20.0% |
| !!! | TTTAATCAATGTATTGATCT+TGG | + | chr3.2:82680218-82680237 | MS.gene055488:intron | 20.0% |
| ! | CAAAATCGAAAACAACAAAA+AGG | - | chr3.2:82678876-82678895 | None:intergenic | 25.0% |
| ! | TTAAAGGAATAAATTCCATG+GGG | - | chr3.2:82679901-82679920 | None:intergenic | 25.0% |
| !! | AATACTTGACAACTCATTTT+AGG | - | chr3.2:82679738-82679757 | None:intergenic | 25.0% |
| !! | AGTAGTACCTAATGTTATTT+GGG | - | chr3.2:82679870-82679889 | None:intergenic | 25.0% |
| !! | AGTTGTCAAGTATTGTTTAT+TGG | + | chr3.2:82679744-82679763 | MS.gene055488:intron | 25.0% |
| !! | CTGAAAACATTTTATGAACA+TGG | + | chr3.2:82679458-82679477 | MS.gene055488:intron | 25.0% |
| !! | TGAAATATATGGAGTATTTG+TGG | + | chr3.2:82679084-82679103 | MS.gene055488:intron | 25.0% |
| !! | TTTTGTGATTCAAATCTGTT+AGG | + | chr3.2:82678891-82678910 | MS.gene055488:intron | 25.0% |
| !!! | ATGAGTTTTTTCTGTTCTAA+TGG | + | chr3.2:82679972-82679991 | MS.gene055488:intron | 25.0% |
| !!! | TAGTTATTTTAAGGCCTTTT+TGG | + | chr3.2:82679331-82679350 | MS.gene055488:intron | 25.0% |
| !!! | TTTTTTGTTTGGTAATCACT+AGG | + | chr3.2:82680022-82680041 | MS.gene055488:intron | 25.0% |
| AACTCTGATCAAATAGTGTA+AGG | - | chr3.2:82679541-82679560 | None:intergenic | 30.0% | |
| AATAAGGATACCAAAAAAGG+AGG | - | chr3.2:82679696-82679715 | None:intergenic | 30.0% | |
| ACTCTGATCAAATAGTGTAA+GGG | - | chr3.2:82679540-82679559 | None:intergenic | 30.0% | |
| CTTATTTCCATTACTTCTAC+AGG | - | chr3.2:82679438-82679457 | None:intergenic | 30.0% | |
| GAGTAGTACCTAATGTTATT+TGG | - | chr3.2:82679871-82679890 | None:intergenic | 30.0% | |
| TGTAATTGAAAGGTTTGTGA+TGG | + | chr3.2:82679110-82679129 | MS.gene055488:intron | 30.0% | |
| TTTGTGGATCTGTAATTGAA+AGG | + | chr3.2:82679100-82679119 | MS.gene055488:intron | 30.0% | |
| ! | ACACTATTTGATCAGAGTTT+TGG | + | chr3.2:82679542-82679561 | MS.gene055488:intron | 30.0% |
| ! | AGCTGCTTCTTTAATTTCAT+CGG | - | chr3.2:82680269-82680288 | None:intergenic | 30.0% |
| ! | GTTTTCTACCTTAAGATGAA+GGG | + | chr3.2:82678745-82678764 | MS.gene055488:CDS | 30.0% |
| ! | TGTCAAGTATTGTTTATTGG+TGG | + | chr3.2:82679747-82679766 | MS.gene055488:intron | 30.0% |
| !! | TGTATTGATCTTGGTTTGTT+TGG | + | chr3.2:82680227-82680246 | MS.gene055488:intron | 30.0% |
| !!! | AATGTGTTCCTTTGTTTTAC+AGG | + | chr3.2:82679136-82679155 | MS.gene055488:intron | 30.0% |
| !!! | AGTTTGGCTCTATTTTATCT+TGG | - | chr3.2:82679410-82679429 | None:intergenic | 30.0% |
| !!! | GTTTGGCTCTATTTTATCTT+GGG | - | chr3.2:82679409-82679428 | None:intergenic | 30.0% |
| ACTTCTACAGGAAAACAGTT+TGG | - | chr3.2:82679426-82679445 | None:intergenic | 35.0% | |
| AGGTCAAGTAGGCAATAATA+AGG | - | chr3.2:82679712-82679731 | None:intergenic | 35.0% | |
| CATCTTAAGGTAGAAAACCT+TGG | - | chr3.2:82678743-82678762 | None:intergenic | 35.0% | |
| TGCAATGTCCTGTAAAACAA+AGG | - | chr3.2:82679147-82679166 | None:intergenic | 35.0% | |
| TGGATTCTACTAACCACTTA+TGG | + | chr3.2:82679664-82679683 | MS.gene055488:intron | 35.0% | |
| ! | CTGTTTTCCTGTAGAAGTAA+TGG | + | chr3.2:82679428-82679447 | MS.gene055488:intron | 35.0% |
| ! | GGTTTTCTACCTTAAGATGA+AGG | + | chr3.2:82678744-82678763 | MS.gene055488:CDS | 35.0% |
| ! | TTTGTTTGGTTTCAGGATGA+TGG | + | chr3.2:82680241-82680260 | MS.gene055488:intron | 35.0% |
| !! | ATCTTGGTTTGTTTGGTTTC+AGG | + | chr3.2:82680234-82680253 | MS.gene055488:intron | 35.0% |
| !! | ATTTTAGGTGTAGGTCAAGT+AGG | - | chr3.2:82679723-82679742 | None:intergenic | 35.0% |
| !! | TGACAACTCATTTTAGGTGT+AGG | - | chr3.2:82679732-82679751 | None:intergenic | 35.0% |
| AACCTGAGCAGATTTGTAAG+CGG | - | chr3.2:82678842-82678861 | None:intergenic | 40.0% | |
| AACCTGTTTAGCAAGATTGC+AGG | - | chr3.2:82679264-82679283 | None:intergenic | 40.0% | |
| AATCAATACCTGCATGTCAG+AGG | - | chr3.2:82680150-82680169 | None:intergenic | 40.0% | |
| AGATGAAGGGAGATTACCAT+AGG | + | chr3.2:82678758-82678777 | MS.gene055488:CDS | 40.0% | |
| GAAGTTATGCTCTTGCTACT+AGG | - | chr3.2:82679949-82679968 | None:intergenic | 40.0% | |
| GAGCTTTCTAACATCTGTGA+CGG | + | chr3.2:82678664-82678683 | MS.gene055488:CDS | 40.0% | |
| GGTAATCTCCCTTCATCTTA+AGG | - | chr3.2:82678756-82678775 | None:intergenic | 40.0% | |
| GTAGAAAACCTTGGAATCAC+CGG | - | chr3.2:82678734-82678753 | None:intergenic | 40.0% | |
| TATCTTGCTGAGTTCAAGAC+TGG | + | chr3.2:82678781-82678800 | MS.gene055488:CDS | 40.0% | |
| TGAACTCAGCAAGATACCTA+TGG | - | chr3.2:82678777-82678796 | None:intergenic | 40.0% | |
| TGATGAAGCAATTGCTGAAC+TGG | + | chr3.2:82680049-82680068 | MS.gene055488:CDS | 40.0% | |
| TTCGTGATAATCTCACCCTA+TGG | + | chr3.2:82680123-82680142 | MS.gene055488:CDS | 40.0% | |
| ! | CTACCGATCTAAGATCGAAT+CGG | + | chr3.2:82678642-82678661 | MS.gene055488:CDS | 40.0% |
| ! | GCAACAATGATGTTGCGTTT+TGG | + | chr3.2:82679644-82679663 | MS.gene055488:intron | 40.0% |
| ! | TGTTTATTGGTGGTTAGCTC+AGG | + | chr3.2:82679757-82679776 | MS.gene055488:intron | 40.0% |
| !!! | ATGGATCAGACCTCCTTTTT+TGG | + | chr3.2:82679683-82679702 | MS.gene055488:intron | 40.0% |
| AAGGAGGTCTGATCCATAAG+TGG | - | chr3.2:82679680-82679699 | None:intergenic | 45.0% | |
| ACCAACTCATCCAATTAGGC+TGG | + | chr3.2:82679181-82679200 | MS.gene055488:CDS | 45.0% | |
| ATGTAAACGTTCTCTTCCCG+AGG | - | chr3.2:82678394-82678413 | None:intergenic | 45.0% | |
| ATTGCTGAACTGGATACCCT+TGG | + | chr3.2:82680059-82680078 | MS.gene055488:CDS | 45.0% | |
| CACCATTTCTTCGTAGCGTT+CGG | - | chr3.2:82678437-82678456 | None:intergenic | 45.0% | |
| CCAACTCATCCAATTAGGCT+GGG | + | chr3.2:82679182-82679201 | MS.gene055488:CDS | 45.0% | |
| CGATCTTAGATCGGTAGTCA+CGG | - | chr3.2:82678639-82678658 | None:intergenic | 45.0% | |
| CTTGTATGATTCCTCGCCAA+GGG | - | chr3.2:82680078-82680097 | None:intergenic | 45.0% | |
| GACGGTGAGTTCTTCGTTAT+CGG | - | chr3.2:82678488-82678507 | None:intergenic | 45.0% | |
| GGTGGTGCCCAAATAACATT+AGG | + | chr3.2:82679860-82679879 | MS.gene055488:intron | 45.0% | |
| GTAGCCTACAAGAACGTCAT+CGG | + | chr3.2:82678529-82678548 | MS.gene055488:CDS | 45.0% | |
| TACTGCTGTTCATCGGGTTT+AGG | - | chr3.2:82680292-82680311 | None:intergenic | 45.0% | |
| TCGGGAAGAGAACGTTTACA+TGG | + | chr3.2:82678393-82678412 | MS.gene055488:CDS | 45.0% | |
| TCTTGTAGGCTACAGAGAGA+AGG | - | chr3.2:82678522-82678541 | None:intergenic | 45.0% | |
| TGCCACCAACTCATCCAATT+AGG | + | chr3.2:82679177-82679196 | MS.gene055488:CDS | 45.0% | |
| TGCCTGCAATCTTGCTAAAC+AGG | + | chr3.2:82679259-82679278 | MS.gene055488:CDS | 45.0% | |
| TTCCTCGATCGAACAGAAAG+AGG | + | chr3.2:82678579-82678598 | MS.gene055488:CDS | 45.0% | |
| ! | AGCCTAATTGGATGAGTTGG+TGG | - | chr3.2:82679182-82679201 | None:intergenic | 45.0% |
| ! | CGAAGAAATGGTGGAGTTCA+TGG | + | chr3.2:82678444-82678463 | MS.gene055488:CDS | 45.0% |
| ! | GCTCCGATTCGATCTTAGAT+CGG | - | chr3.2:82678648-82678667 | None:intergenic | 45.0% |
| AACACCTTGCTCTAGCCTGT+AGG | + | chr3.2:82679833-82679852 | MS.gene055488:intron | 50.0% | |
| AAGAACTCACCGTCGAAGAG+AGG | + | chr3.2:82678494-82678513 | MS.gene055488:CDS | 50.0% | |
| AAGCAGCGGAAGGAATGAGA+CGG | - | chr3.2:82678711-82678730 | None:intergenic | 50.0% | |
| ACACCTTGCTCTAGCCTGTA+GGG | + | chr3.2:82679834-82679853 | MS.gene055488:intron | 50.0% | |
| AGAGCAAGACCCAGCCTAAT+TGG | - | chr3.2:82679194-82679213 | None:intergenic | 50.0% | |
| AGATCGGTAGTCACGGATGA+CGG | - | chr3.2:82678632-82678651 | None:intergenic | 50.0% | |
| AGCCGAACGCTACGAAGAAA+TGG | + | chr3.2:82678432-82678451 | MS.gene055488:CDS | 50.0% | |
| CCCAGCCTAATTGGATGAGT+TGG | - | chr3.2:82679185-82679204 | None:intergenic | 50.0% | |
| CCTTGGCGAGGAATCATACA+AGG | + | chr3.2:82680076-82680095 | MS.gene055488:CDS | 50.0% | |
| CCTTGTATGATTCCTCGCCA+AGG | - | chr3.2:82680079-82680098 | None:intergenic | 50.0% | |
| CGAACGCTACGAAGAAATGG+TGG | + | chr3.2:82678435-82678454 | MS.gene055488:CDS | 50.0% | |
| CTCCTCTTTCTGTTCGATCG+AGG | - | chr3.2:82678584-82678603 | None:intergenic | 50.0% | |
| CTGCATGTCAGAGGTCCATA+GGG | - | chr3.2:82680141-82680160 | None:intergenic | 50.0% | |
| GAGAAGGTTCCTCTCTTCGA+CGG | - | chr3.2:82678506-82678525 | None:intergenic | 50.0% | |
| GAGTTCTTCGTTATCGGCGT+TGG | - | chr3.2:82678482-82678501 | None:intergenic | 50.0% | |
| TACTACTCGTGTAGTCCCCA+TGG | + | chr3.2:82679883-82679902 | MS.gene055488:intron | 50.0% | |
| TGCTCCGATGACGTTCTTGT+AGG | - | chr3.2:82678536-82678555 | None:intergenic | 50.0% | |
| ! | TTCGTAGCGTTCGGCTTGTT+CGG | - | chr3.2:82678428-82678447 | None:intergenic | 50.0% |
| ! | TTCTTCGTTATCGGCGTTGG+CGG | - | chr3.2:82678479-82678498 | None:intergenic | 50.0% |
| AAACGTGATCCTCGTTGCCG+CGG | - | chr3.2:82678609-82678628 | None:intergenic | 55.0% | |
| AGCAGCGGAAGGAATGAGAC+GGG | - | chr3.2:82678710-82678729 | None:intergenic | 55.0% | |
| AGTCCCTACAGGCTAGAGCA+AGG | - | chr3.2:82679840-82679859 | None:intergenic | 55.0% | |
| CCTATGGACCTCTGACATGC+AGG | + | chr3.2:82680139-82680158 | MS.gene055488:CDS | 55.0% | |
| CCTGCATGTCAGAGGTCCAT+AGG | - | chr3.2:82680142-82680161 | None:intergenic | 55.0% | |
| CGCCGCTTACAAATCTGCTC+AGG | + | chr3.2:82678837-82678856 | MS.gene055488:CDS | 55.0% | |
| CTCATTCCTTCCGCTGCTTC+CGG | + | chr3.2:82678712-82678731 | MS.gene055488:CDS | 55.0% | |
| CTCTTCCCGAGGTGAATGAG+CGG | - | chr3.2:82678383-82678402 | None:intergenic | 55.0% | |
| CTTGGAATCACCGGAAGCAG+CGG | - | chr3.2:82678725-82678744 | None:intergenic | 55.0% | |
| GAACAGAAAGAGGAGAGCCG+CGG | + | chr3.2:82678589-82678608 | MS.gene055488:CDS | 55.0% | |
| GCAAGATTGCAGGCACGATC+AGG | - | chr3.2:82679254-82679273 | None:intergenic | 55.0% | |
| TGAACTGGATACCCTTGGCG+AGG | + | chr3.2:82680064-82680083 | MS.gene055488:CDS | 55.0% | |
| TTGCTCTAGCCTGTAGGGAC+TGG | + | chr3.2:82679839-82679858 | MS.gene055488:intron | 55.0% | |
| CGCTGCTTCCGGTGATTCCA+AGG | + | chr3.2:82678723-82678742 | MS.gene055488:CDS | 60.0% | |
| CTCAGCAGCCTCCTTACGCT+CGG | - | chr3.2:82678809-82678828 | None:intergenic | 60.0% | |
| CTCTAGCCTGTAGGGACTGG+TGG | + | chr3.2:82679842-82679861 | MS.gene055488:intron | 60.0% | |
| GAATCACCGGAAGCAGCGGA+AGG | - | chr3.2:82678721-82678740 | None:intergenic | 60.0% | |
| TCGGAGCACGACGTGCTTCG+TGG | + | chr3.2:82678548-82678567 | MS.gene055488:CDS | 65.0% | |
| TGGCGGCCGCTCATTCACCT+CGG | + | chr3.2:82678374-82678393 | MS.gene055488:CDS | 65.0% | |
| TGGGCACCACCAGTCCCTAC+AGG | - | chr3.2:82679851-82679870 | None:intergenic | 65.0% | |
| ! | CAAGACTGGCGCCGAGCGTA+AGG | + | chr3.2:82678795-82678814 | MS.gene055488:CDS | 65.0% |
| AGAGGAGAGCCGCGGCAACG+AGG | + | chr3.2:82678597-82678616 | MS.gene055488:CDS | 70.0% | |
| GGCGGCCGCTCATTCACCTC+GGG | + | chr3.2:82678375-82678394 | MS.gene055488:CDS | 70.0% | |
| ! | GACTGGCGCCGAGCGTAAGG+AGG | + | chr3.2:82678798-82678817 | MS.gene055488:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 82678373 | 82680312 | 82678373 | ID=MS.gene055488 |
| chr3.2 | mRNA | 82678373 | 82680312 | 82678373 | ID=MS.gene055488.t1;Parent=MS.gene055488 |
| chr3.2 | exon | 82678373 | 82678858 | 82678373 | ID=MS.gene055488.t1.exon1;Parent=MS.gene055488.t1 |
| chr3.2 | CDS | 82678373 | 82678858 | 82678373 | ID=cds.MS.gene055488.t1;Parent=MS.gene055488.t1 |
| chr3.2 | exon | 82679158 | 82679280 | 82679158 | ID=MS.gene055488.t1.exon2;Parent=MS.gene055488.t1 |
| chr3.2 | CDS | 82679158 | 82679280 | 82679158 | ID=cds.MS.gene055488.t1;Parent=MS.gene055488.t1 |
| chr3.2 | exon | 82680044 | 82680160 | 82680044 | ID=MS.gene055488.t1.exon3;Parent=MS.gene055488.t1 |
| chr3.2 | CDS | 82680044 | 82680160 | 82680044 | ID=cds.MS.gene055488.t1;Parent=MS.gene055488.t1 |
| chr3.2 | exon | 82680256 | 82680312 | 82680256 | ID=MS.gene055488.t1.exon4;Parent=MS.gene055488.t1 |
| chr3.2 | CDS | 82680256 | 82680312 | 82680256 | ID=cds.MS.gene055488.t1;Parent=MS.gene055488.t1 |
| Gene Sequence |
| Protein sequence |
