AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene041779

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene041779.t1 MTR_1g095540 95.852 458 19 0 1 458 1 458 0.0 924
MS.gene041779.t1 MTR_1g095560 84.934 458 69 0 1 458 1 458 0.0 816
MS.gene041779.t1 MTR_1g095560 79.592 490 68 2 1 458 1 490 0.0 801
MS.gene041779.t1 MTR_2g062990 53.829 457 205 5 5 457 9 463 0.0 515
MS.gene041779.t1 MTR_2g063000 52.575 466 210 5 1 457 1 464 1.20e-178 508
MS.gene041779.t1 MTR_7g013790 54.130 460 196 8 8 457 11 465 2.24e-176 502
MS.gene041779.t1 MTR_2g062980 52.298 457 212 5 5 457 9 463 2.41e-175 500
MS.gene041779.t1 MTR_2g081620 52.434 452 208 6 8 455 14 462 8.85e-174 496
MS.gene041779.t1 MTR_4g075150 49.670 455 218 8 9 456 11 461 2.93e-154 446
MS.gene041779.t1 MTR_2g063000 48.069 466 200 6 1 457 1 433 4.61e-154 444
MS.gene041779.t1 MTR_4g075150 49.672 457 217 8 9 456 11 463 8.45e-154 445
MS.gene041779.t1 MTR_3g079560 52.668 431 191 7 29 458 74 492 1.23e-153 446
MS.gene041779.t1 MTR_3g079570 52.184 435 194 7 26 458 70 492 9.68e-152 441
MS.gene041779.t1 MTR_3g034290 48.837 430 210 5 28 456 70 490 2.28e-149 435
MS.gene041779.t1 MTR_3g079590 51.991 427 192 7 26 450 69 484 1.34e-148 432
MS.gene041779.t1 MTR_3g079600 52.113 426 190 6 28 450 71 485 1.44e-147 430
MS.gene041779.t1 MTR_3g079620 50.572 437 195 7 31 458 76 500 4.75e-147 429
MS.gene041779.t1 MTR_1g021010 49.083 436 205 7 17 450 60 480 2.36e-143 419
MS.gene041779.t1 MTR_1g020090 48.513 437 203 7 19 450 72 491 7.63e-141 413
MS.gene041779.t1 MTR_3g079630 50.116 431 190 8 31 450 76 492 3.72e-140 411
MS.gene041779.t1 MTR_4g122950 44.211 475 241 6 5 458 8 479 6.66e-139 407
MS.gene041779.t1 MTR_4g122960 44.397 473 241 7 5 458 8 477 1.22e-138 407
MS.gene041779.t1 MTR_4g089155 46.818 440 218 6 31 458 52 487 1.32e-138 407
MS.gene041779.t1 MTR_0003s0560 46.188 446 224 9 14 457 57 488 3.82e-137 403
MS.gene041779.t1 MTR_1g021020 47.597 437 209 7 17 450 62 481 7.02e-137 402
MS.gene041779.t1 MTR_2g012860 43.841 479 236 8 3 457 5 474 4.00e-136 400
MS.gene041779.t1 MTR_2g012850 43.497 469 246 8 3 458 5 467 7.94e-132 389
MS.gene041779.t1 MTR_3g034290 49.138 348 168 4 28 375 70 408 9.26e-120 356
MS.gene041779.t1 MTR_2g449850 40.858 443 238 9 28 458 34 464 1.54e-110 334
MS.gene041779.t1 MTR_3g498755 40.816 441 238 12 31 456 13 445 2.36e-108 328
MS.gene041779.t1 MTR_8g099930 39.659 469 248 13 12 458 9 464 4.63e-108 328
MS.gene041779.t1 MTR_3g434850 40.085 469 254 12 1 457 2 455 1.10e-107 327
MS.gene041779.t1 MTR_5g072570 40.590 441 246 8 30 458 27 463 1.63e-105 322
MS.gene041779.t1 MTR_3g090130 41.781 438 241 9 30 458 26 458 3.70e-105 320
MS.gene041779.t1 MTR_7g021130 40.319 439 239 12 31 456 29 457 8.49e-105 319
MS.gene041779.t1 MTR_3g090150 39.269 438 254 8 30 458 31 465 2.26e-100 308
MS.gene041779.t1 MTR_1g040370 36.933 463 233 14 2 456 4 415 6.26e-95 293
MS.gene041779.t1 MTR_0003s0560 48.188 276 136 5 14 289 57 325 3.96e-84 263
MS.gene041779.t1 MTR_8g011350 31.791 497 281 12 2 450 4 490 9.07e-75 243
MS.gene041779.t1 MTR_7g051430 33.400 500 273 16 1 450 1 490 5.97e-72 236
MS.gene041779.t1 MTR_7g080180 32.985 479 257 15 33 455 28 498 2.73e-68 226
MS.gene041779.t1 MTR_1g102130 31.933 476 255 15 6 456 59 490 8.05e-67 222
MS.gene041779.t1 MTR_8g011350 30.737 475 271 12 2 428 4 468 8.91e-65 216
MS.gene041779.t1 MTR_7g080180 31.808 437 235 14 33 414 28 456 3.96e-55 190
MS.gene041779.t1 MTR_2g018600 28.795 448 282 15 33 455 34 469 6.89e-54 187
MS.gene041779.t1 MTR_7g051430 32.311 424 228 15 1 375 1 414 3.65e-52 181
MS.gene041779.t1 MTR_2g018590 28.118 473 303 14 5 455 12 469 7.36e-52 182
MS.gene041779.t1 MTR_4g021535 28.387 465 304 13 6 452 10 463 1.55e-51 181
MS.gene041779.t1 MTR_4g021520 29.385 439 282 12 32 452 39 467 2.23e-51 181
MS.gene041779.t1 MTR_4g021500 30.700 443 278 14 32 455 39 471 1.07e-50 184
MS.gene041779.t1 MTR_4g021500 28.947 418 267 13 45 452 487 884 3.62e-45 169
MS.gene041779.t1 MTR_4g021500 30.700 443 278 14 32 455 39 471 1.48e-50 184
MS.gene041779.t1 MTR_4g021500 28.704 432 268 14 45 452 487 902 2.31e-44 166
MS.gene041779.t1 MTR_4g021490 29.385 439 282 14 32 452 39 467 1.71e-50 178
MS.gene041779.t1 MTR_4g021510 28.889 450 270 15 32 452 38 466 1.98e-50 178
MS.gene041779.t1 MTR_3g100420 29.019 479 296 16 5 452 28 493 5.21e-50 177
MS.gene041779.t1 MTR_4g021530 28.604 444 289 11 27 452 34 467 8.76e-50 176
MS.gene041779.t1 MTR_3g045770 28.241 432 281 11 33 447 27 446 1.02e-47 170
MS.gene041779.t1 MTR_2g018630 27.273 440 292 13 33 455 37 465 2.05e-45 164
MS.gene041779.t1 MTR_4g021520 29.250 400 256 11 32 414 39 428 3.02e-42 155
MS.gene041779.t1 MTR_0075s0010 31.688 385 180 16 30 402 5 318 1.86e-40 147
MS.gene041779.t1 MTR_2g095730 26.950 423 278 12 44 453 91 495 1.53e-39 149
MS.gene041779.t1 MTR_1g050545 28.537 417 262 15 45 447 81 475 4.82e-38 145
MS.gene041779.t1 MTR_4g057585 26.606 436 265 12 38 450 87 490 9.06e-38 144
MS.gene041779.t1 MTR_2g095750 25.874 429 272 12 44 453 97 498 3.38e-37 142
MS.gene041779.t1 MTR_5g097180 27.857 420 265 13 49 455 43 437 3.70e-37 142
MS.gene041779.t1 MTR_4g084710 28.132 423 260 15 49 455 45 439 4.03e-37 141
MS.gene041779.t1 MTR_4g057570 25.056 443 285 12 32 453 74 490 1.44e-36 140
MS.gene041779.t1 MTR_4g057595 25.977 435 267 12 40 451 90 492 4.14e-36 139
MS.gene041779.t1 MTR_4g057600 26.279 430 262 14 47 453 95 492 5.25e-35 136
MS.gene041779.t1 MTR_1g094155 26.988 415 272 14 44 447 87 481 1.40e-34 135
MS.gene041779.t1 MTR_4g021535 25.806 465 269 16 6 452 10 416 2.07e-34 133
MS.gene041779.t1 MTR_2g009200 26.271 472 283 15 6 450 1 434 1.30e-33 131
MS.gene041779.t1 MTR_2g018630 25.424 354 244 10 112 453 13 358 1.16e-29 119
MS.gene041779.t1 MTR_2g095710 25.240 416 247 13 62 453 106 481 2.99e-26 111
MS.gene041779.t1 MTR_1g051175 41.085 129 64 5 27 150 2 123 1.25e-18 86.3
MS.gene041779.t1 MTR_4g056520 22.523 444 297 15 41 452 27 455 6.63e-16 80.1
MS.gene041779.t1 MTR_2g084530 23.684 418 278 16 50 439 36 440 2.13e-11 66.2
MS.gene041779.t1 MTR_4g122970 38.554 83 41 2 8 82 7 87 8.04e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene041779.t1 AT3G07990 72.874 435 118 0 22 456 23 457 0.0 677
MS.gene041779.t1 AT2G35780 68.506 435 131 3 24 456 21 451 0.0 634
MS.gene041779.t1 AT4G30610 55.224 469 194 7 1 458 1 464 0.0 531
MS.gene041779.t1 AT2G24000 56.152 447 183 6 22 458 20 463 1.31e-180 513
MS.gene041779.t1 AT2G35770 54.803 458 193 7 5 458 13 460 6.13e-175 498
MS.gene041779.t1 AT2G24010 54.943 435 191 4 25 458 23 453 1.74e-173 494
MS.gene041779.t1 AT3G02110 54.464 448 184 7 26 458 31 473 1.43e-172 493
MS.gene041779.t1 AT4G30810 54.060 431 193 2 23 450 28 456 1.02e-171 491
MS.gene041779.t1 AT2G24010 52.889 450 192 5 25 458 23 468 2.78e-168 482
MS.gene041779.t1 AT2G24010 50.321 467 187 6 33 458 2 464 1.36e-159 459
MS.gene041779.t1 AT5G23210 49.563 458 201 8 25 457 44 496 3.75e-151 439
MS.gene041779.t1 AT3G63470 47.826 437 208 8 28 458 77 499 9.55e-147 428
MS.gene041779.t1 AT1G61130 46.253 467 236 9 1 458 2 462 4.88e-138 405
MS.gene041779.t1 AT3G17180 46.889 450 220 8 25 458 31 477 1.53e-136 401
MS.gene041779.t1 AT5G08260 42.469 478 242 11 5 457 8 477 2.18e-134 396
MS.gene041779.t1 AT5G23210 49.265 408 177 8 25 407 44 446 2.78e-131 387
MS.gene041779.t1 AT1G11080 45.595 454 224 6 24 458 42 491 4.51e-131 388
MS.gene041779.t1 AT1G11080 45.595 454 224 6 24 458 42 491 5.35e-131 388
MS.gene041779.t1 AT3G52020 46.560 436 218 11 26 456 73 498 1.38e-129 384
MS.gene041779.t1 AT4G15100 43.451 481 244 9 2 458 7 483 5.62e-128 380
MS.gene041779.t1 AT2G24010 52.925 359 149 5 33 375 2 356 4.98e-127 373
MS.gene041779.t1 AT5G23210 47.891 403 183 6 80 457 1 401 2.06e-125 370
MS.gene041779.t1 AT5G23210 48.101 395 178 6 88 457 8 400 3.32e-124 367
MS.gene041779.t1 AT2G05850 42.282 447 241 9 19 458 50 486 5.39e-119 357
MS.gene041779.t1 AT3G17180 45.161 403 203 7 25 412 31 430 2.47e-117 351
MS.gene041779.t1 AT3G52000 43.243 444 236 12 19 458 50 481 2.30e-116 350
MS.gene041779.t1 AT3G52010 41.704 446 240 11 21 458 53 486 1.51e-115 348
MS.gene041779.t1 AT2G33530 43.363 452 227 13 22 456 23 462 4.65e-111 336
MS.gene041779.t1 AT1G28110 40.816 441 234 11 31 456 30 458 2.55e-104 318
MS.gene041779.t1 AT1G28110 40.816 441 234 11 31 456 30 458 2.55e-104 318
MS.gene041779.t1 AT5G23210 47.536 345 154 6 88 407 8 350 2.76e-104 315
MS.gene041779.t1 AT1G43780 40.271 442 243 9 30 456 35 470 1.05e-103 317
MS.gene041779.t1 AT5G42240 40.271 442 248 8 29 458 29 466 3.78e-102 313
MS.gene041779.t1 AT2G12480 38.621 435 257 6 29 457 28 458 4.00e-102 313
MS.gene041779.t1 AT2G24000 41.163 447 187 9 22 458 20 400 5.63e-102 310
MS.gene041779.t1 AT2G12480 37.788 434 238 7 29 457 28 434 1.75e-97 300
MS.gene041779.t1 AT2G12480 37.558 434 237 7 29 457 28 432 1.17e-96 298
MS.gene041779.t1 AT2G12480 37.471 435 237 8 29 457 28 433 3.71e-95 294
MS.gene041779.t1 AT5G42230 37.188 441 261 8 29 457 25 461 4.20e-94 292
MS.gene041779.t1 AT5G42230 37.188 441 261 8 29 457 25 461 4.20e-94 292
MS.gene041779.t1 AT4G12910 32.929 495 276 12 8 455 12 497 5.95e-79 254
MS.gene041779.t1 AT3G25420 32.454 493 275 13 14 455 9 494 4.74e-75 244
MS.gene041779.t1 AT3G56540 62.222 180 64 3 10 189 57 232 4.14e-73 231
MS.gene041779.t1 AT4G12910 31.788 453 254 11 8 414 12 455 2.01e-65 217
MS.gene041779.t1 AT2G22990 31.555 431 263 13 33 453 23 431 1.10e-59 202
MS.gene041779.t1 AT2G22970 30.947 433 265 13 33 455 25 433 2.15e-57 196
MS.gene041779.t1 AT2G22920 30.645 434 267 14 33 455 25 435 2.76e-56 192
MS.gene041779.t1 AT2G22990 31.373 408 249 12 33 430 23 409 1.11e-53 186
MS.gene041779.t1 AT2G22990 31.450 407 248 12 33 429 23 408 1.21e-53 185
MS.gene041779.t1 AT2G23000 28.538 431 278 13 33 453 25 435 2.55e-52 182
MS.gene041779.t1 AT2G23000 28.538 431 278 13 33 453 25 435 2.55e-52 182
MS.gene041779.t1 AT2G22990 30.023 433 262 14 33 455 23 424 8.49e-52 181
MS.gene041779.t1 AT1G73270 30.575 435 264 14 33 455 33 441 1.52e-51 180
MS.gene041779.t1 AT1G73270 30.575 435 264 14 33 455 33 441 1.82e-51 180
MS.gene041779.t1 AT1G73280 29.797 443 257 15 33 455 33 441 1.89e-51 180
MS.gene041779.t1 AT2G22970 30.144 418 259 12 33 440 25 419 6.44e-51 179
MS.gene041779.t1 AT1G73300 30.162 431 271 12 33 455 33 441 9.95e-51 178
MS.gene041779.t1 AT1G73300 30.162 431 271 12 33 455 33 441 9.95e-51 178
MS.gene041779.t1 AT2G23010 29.213 445 285 14 19 453 11 435 2.10e-50 177
MS.gene041779.t1 AT2G22970 30.392 408 251 12 33 430 25 409 3.33e-50 176
MS.gene041779.t1 AT2G23010 28.989 445 286 14 19 453 11 435 6.49e-50 176
MS.gene041779.t1 AT1G73290 29.358 436 266 13 33 455 32 438 1.18e-49 175
MS.gene041779.t1 AT3G10450 28.472 432 277 12 33 455 29 437 7.06e-48 170
MS.gene041779.t1 AT5G36180 30.070 429 270 12 33 453 33 439 1.47e-47 170
MS.gene041779.t1 AT3G12203 28.472 432 276 11 33 455 30 437 1.66e-47 169
MS.gene041779.t1 AT3G12240 28.802 434 273 14 33 455 28 436 1.68e-47 169
MS.gene041779.t1 AT3G12203 28.472 432 276 11 33 455 9 416 1.72e-47 169
MS.gene041779.t1 AT2G22920 30.534 393 241 12 33 415 25 395 1.84e-47 169
MS.gene041779.t1 AT2G22980 28.972 428 266 12 33 450 26 425 5.29e-47 168
MS.gene041779.t1 AT5G09640 26.283 487 301 13 1 453 1 463 7.00e-47 168
MS.gene041779.t1 AT3G12230 28.571 434 274 13 33 455 27 435 1.75e-46 167
MS.gene041779.t1 AT1G33540 27.865 445 290 13 33 455 29 464 3.97e-46 166
MS.gene041779.t1 AT5G22980 28.770 431 263 14 40 453 91 494 5.87e-45 164
MS.gene041779.t1 AT3G12220 28.571 434 270 12 33 453 27 433 1.56e-44 161
MS.gene041779.t1 AT3G10450 28.155 412 264 12 51 453 4 392 1.62e-44 160
MS.gene041779.t1 AT1G73310 28.211 436 269 13 33 453 33 439 2.02e-44 161
MS.gene041779.t1 AT1G33540 27.635 427 278 13 51 455 4 421 2.18e-43 158
MS.gene041779.t1 AT1G73290 29.114 395 240 11 33 415 32 398 6.99e-42 153
MS.gene041779.t1 AT2G22980 27.990 418 264 11 33 440 26 416 8.80e-42 154
MS.gene041779.t1 AT3G12203 28.180 401 255 11 62 453 1 377 9.10e-42 152
MS.gene041779.t1 AT2G22990 31.250 336 207 10 123 453 1 317 1.28e-41 150
MS.gene041779.t1 AT2G22980 28.431 408 255 11 33 430 26 406 2.76e-41 152
MS.gene041779.t1 AT3G10450 28.010 407 262 11 33 430 29 413 3.92e-41 152
MS.gene041779.t1 AT2G22990 30.952 336 208 10 123 453 1 317 5.16e-41 149
MS.gene041779.t1 AT3G12220 27.885 416 260 12 51 453 15 403 3.71e-40 149
MS.gene041779.t1 AT3G10410 26.995 426 279 11 38 452 90 494 5.36e-40 150
MS.gene041779.t1 AT3G45010 26.790 433 275 12 38 453 92 499 1.02e-39 150
MS.gene041779.t1 AT1G73310 28.293 410 250 12 33 427 33 413 3.72e-39 147
MS.gene041779.t1 AT3G10450 27.506 389 251 11 51 430 4 370 1.23e-37 141
MS.gene041779.t1 AT2G22920 31.343 268 169 7 33 291 25 286 2.31e-37 139
MS.gene041779.t1 AT2G22970 31.343 268 168 7 33 291 25 285 3.78e-37 139
MS.gene041779.t1 AT1G73280 32.342 269 164 7 33 287 33 297 2.34e-36 138
MS.gene041779.t1 AT1G73290 32.083 240 154 4 33 267 32 267 8.93e-36 134
MS.gene041779.t1 AT1G73280 27.731 357 212 12 112 453 12 337 5.79e-35 133
MS.gene041779.t1 AT1G73280 27.697 343 202 12 126 453 1 312 2.46e-32 125
MS.gene041779.t1 AT5G36180 28.406 345 225 9 112 453 12 337 3.23e-31 123
MS.gene041779.t1 AT5G36180 28.406 345 225 9 112 453 12 337 3.23e-31 123
MS.gene041779.t1 AT1G15000 27.889 398 254 11 49 438 37 409 1.14e-29 120
MS.gene041779.t1 AT2G22980 30.597 268 168 7 33 291 26 284 2.28e-29 118
MS.gene041779.t1 AT3G12220 32.877 219 136 5 33 245 27 240 5.33e-28 115
MS.gene041779.t1 AT5G36180 27.640 322 212 8 112 430 12 315 5.63e-26 108
MS.gene041779.t1 AT5G36180 26.765 340 217 8 112 437 12 333 2.32e-24 103
MS.gene041779.t1 AT2G22980 26.518 313 201 8 123 430 1 289 1.06e-23 101
MS.gene041779.t1 AT1G73310 26.380 326 204 9 112 427 12 311 2.50e-23 100
MS.gene041779.t1 AT5G22960 29.851 201 105 5 11 211 10 174 1.07e-22 95.5
MS.gene041779.t1 AT2G22920 27.473 273 173 10 189 455 3 256 3.44e-22 95.9
MS.gene041779.t1 AT2G27920 23.555 467 295 21 9 439 5 445 1.18e-17 85.5
MS.gene041779.t1 AT2G22960 30.534 131 86 4 324 453 56 182 7.65e-14 70.1

Find 99 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAAACATTGGCTGTGATTT+TGG 0.097316 1.1:-68859644 MS.gene041779:CDS
GATGATTATCATGATTATAT+TGG 0.232593 1.1:-68859722 MS.gene041779:CDS
TTACTGTGTGGGAATTTCTT+TGG 0.247907 1.1:-68861377 MS.gene041779:CDS
AAACAACACAACACAGAATA+TGG 0.249064 1.1:+68861408 None:intergenic
GTTGAATTTGAACAGTATTC+AGG 0.256370 1.1:-68861292 MS.gene041779:CDS
AGAAATTCCCACACAGTAAA+TGG 0.257794 1.1:+68861381 None:intergenic
GTGGTACTGTTTGGGATTAT+TGG 0.264427 1.1:-68859137 MS.gene041779:CDS
GGTTGTTCTTCCATTGCTTA+TGG 0.265738 1.1:-68861154 MS.gene041779:CDS
AATTGTTAACCAGAGAAATA+AGG 0.271531 1.1:-68859895 MS.gene041779:CDS
GCACTTCATGCCAATGTAAC+TGG 0.277277 1.1:-68859290 MS.gene041779:CDS
TAAAACTAGTGGCTTTGAAT+TGG 0.298079 1.1:+68861195 None:intergenic
TTCTTGACAGTGGTACTGTT+TGG 0.300868 1.1:-68859146 MS.gene041779:intron
TGAAGTGCCTTCTGAACTTC+TGG 0.312729 1.1:+68859305 None:intergenic
AATCCCTTAAAATTTATAAC+TGG 0.318228 1.1:+68859861 None:intergenic
AATGCATCTGATTAACTTGA+TGG 0.320292 1.1:+68858173 None:intergenic
TCTTGACAGTGGTACTGTTT+GGG 0.320433 1.1:-68859145 MS.gene041779:intron
TTAATAAGTTCTTGATATAT+AGG 0.324336 1.1:+68859090 None:intergenic
TATGGTGCTTCTGAAGAAAT+TGG 0.325012 1.1:-68861136 MS.gene041779:CDS
GGATGGAGTCAAGTTTATAA+AGG 0.364164 1.1:-68858299 MS.gene041779:CDS
TACAGATTCCCTTATTTCTC+TGG 0.376213 1.1:+68859886 None:intergenic
TCCATCAGTGCAATGTTTGC+AGG 0.379315 1.1:-68859609 MS.gene041779:CDS
TTACAATTGTTCCAAGCATA+AGG 0.383194 1.1:+68861070 None:intergenic
TGGTACCCTTGGTATGATAA+TGG 0.388411 1.1:-68858577 MS.gene041779:CDS
AAAACTAGTGGCTTTGAATT+GGG 0.395531 1.1:+68861196 None:intergenic
ACTACAACTCGATATTCTAT+TGG 0.399865 1.1:-68858628 MS.gene041779:CDS
CTTGAGTCTCCTGCTGGTGT+TGG 0.407364 1.1:-68860935 MS.gene041779:CDS
AATGTTTAGCTTCTTATATG+TGG 0.413578 1.1:+68859658 None:intergenic
TTATGGCTTAATGGCGGTCC+TGG 0.433602 1.1:-68861175 MS.gene041779:CDS
CATAGAGAATTCTACATGGC+TGG 0.440477 1.1:-68860057 MS.gene041779:CDS
ATTGTTAACCAGAGAAATAA+GGG 0.444696 1.1:-68859894 MS.gene041779:CDS
TTTACTTGAATCCTTATGCT+TGG 0.446628 1.1:-68861081 MS.gene041779:CDS
ATATTGGAACATTTGAGTAC+TGG 0.448006 1.1:-68859706 MS.gene041779:CDS
GATGATGGTTGGTAGCTTCA+AGG 0.449314 1.1:+68858603 None:intergenic
AACTTATTAATGCCGGTCTC+CGG 0.457058 1.1:-68859077 MS.gene041779:CDS
GATGGAGTCAAGTTTATAAA+GGG 0.457624 1.1:-68858298 MS.gene041779:CDS
ACTGAGCAAGGAAACATTGA+TGG 0.464521 1.1:-68859569 MS.gene041779:CDS
CCATCATCAATTGGTACCCT+TGG 0.465365 1.1:-68858588 MS.gene041779:CDS
GATAAAATTAAGTGGTTACC+AGG 0.466526 1.1:-68861328 MS.gene041779:CDS
ACATTTGGTGACCAGAAAAC+AGG 0.467712 1.1:-68860878 MS.gene041779:intron
TAACTGGGATTTCATATGCT+TGG 0.468731 1.1:-68859274 MS.gene041779:CDS
ATATAGGAAGCATAGACAAT+GGG 0.471053 1.1:+68859106 None:intergenic
GTCTCCGGACATGGGTATAC+AGG 0.472161 1.1:-68859062 MS.gene041779:intron
CTCACATTGGTCACAGTAAG+AGG 0.474837 1.1:-68858275 MS.gene041779:CDS
CATATGCTTGGAAGGCATGC+AGG 0.479209 1.1:-68859262 MS.gene041779:intron
AAGTAAAGTGACTTGCCATC+AGG 0.482213 1.1:+68861097 None:intergenic
ATAGAGAATTCTACATGGCT+GGG 0.484144 1.1:-68860056 MS.gene041779:CDS
AGCTACCAACCATCATCAAT+TGG 0.484161 1.1:-68858597 MS.gene041779:CDS
ACAACAGATATGCAGACATT+TGG 0.486962 1.1:-68860893 MS.gene041779:CDS
GCCTGCAAACATTGCACTGA+TGG 0.490912 1.1:+68859608 None:intergenic
CTGTTCCTTGAGTCTCCTGC+TGG 0.494328 1.1:-68860941 MS.gene041779:CDS
AGGTCATTATGTTCTTCAGT+TGG 0.495687 1.1:-68859922 MS.gene041779:CDS
TTAATGCCGGTCTCCGGACA+TGG 0.511891 1.1:-68859071 MS.gene041779:CDS
CACTTCATGCCAATGTAACT+GGG 0.519280 1.1:-68859289 MS.gene041779:CDS
TTCAGAAGCACCATAAGCAA+TGG 0.524508 1.1:+68861144 None:intergenic
TGGGATTTCATATGCTTGGA+AGG 0.530564 1.1:-68859270 MS.gene041779:CDS
AAAAGAGAGATAAAATTAAG+TGG 0.531113 1.1:-68861336 MS.gene041779:CDS
TATCAAGAACTTATTAATGC+CGG 0.540455 1.1:-68859084 MS.gene041779:CDS
TTGGTCACAGTAAGAGGTGC+TGG 0.540636 1.1:-68858269 MS.gene041779:CDS
CCATCAGGCCTAATACGAAA+AGG 0.543223 1.1:+68861112 None:intergenic
ACAGTTGCAATTACTGAGCA+AGG 0.545839 1.1:-68859581 MS.gene041779:CDS
ATGGCTGGGGAAAGTTATGC+AGG 0.552095 1.1:-68860042 MS.gene041779:intron
GGTACCTGTATACCCATGTC+CGG 0.557023 1.1:+68859058 None:intergenic
TTGCACTGATGGATGCTGAA+TGG 0.559164 1.1:+68859619 None:intergenic
CAACACCAGCAGGAGACTCA+AGG 0.569154 1.1:+68860936 None:intergenic
GAATATCGAGTTGTAGTCAG+TGG 0.570502 1.1:+68858634 None:intergenic
CCAAGGGTACCAATTGATGA+TGG 0.575493 1.1:+68858588 None:intergenic
ATATGAAATCCCAGTTACAT+TGG 0.575710 1.1:+68859280 None:intergenic
GTATACCCATGTCCGGAGAC+CGG 0.578010 1.1:+68859065 None:intergenic
TTGGAACATTTGAGTACTGG+TGG 0.579804 1.1:-68859703 MS.gene041779:CDS
ACTGAAGCACCACTGAGTCA+TGG 0.585903 1.1:-68861220 MS.gene041779:CDS
TCAGAATACCTTTCAGCACA+TGG 0.589369 1.1:+68859344 None:intergenic
CATATGATCCATGTGCTGAA+AGG 0.595074 1.1:-68859352 MS.gene041779:CDS
TTTGAGTACTGGTGGACACA+TGG 0.597648 1.1:-68859695 MS.gene041779:CDS
CATTCGTCCAGAAGTTCAGA+AGG 0.600186 1.1:-68859312 MS.gene041779:CDS
TCATATGCTCTATACATCCA+AGG 0.600430 1.1:+68859368 None:intergenic
GGGTACCAATTGATGATGGT+TGG 0.601605 1.1:+68858592 None:intergenic
TAGAGAATTCTACATGGCTG+GGG 0.605545 1.1:-68860055 MS.gene041779:CDS
TATATAGGAAGCATAGACAA+TGG 0.610027 1.1:+68859105 None:intergenic
AGTAAGAGGTGCTGGACATG+AGG 0.612576 1.1:-68858261 MS.gene041779:CDS
TAATGCCGGTCTCCGGACAT+GGG 0.613778 1.1:-68859070 MS.gene041779:CDS
ACATATAAGAAGCTAAACAT+TGG 0.621226 1.1:-68859656 MS.gene041779:CDS
ACCTTGCCATTATCATACCA+AGG 0.630379 1.1:+68858571 None:intergenic
GCTTCACGAGGCTTATGAAG+AGG 0.634672 1.1:+68858236 None:intergenic
AATTCTCTATGTTTGTACTG+AGG 0.640776 1.1:+68860069 None:intergenic
AGTGATGTGGTATTGTTACA+AGG 0.643653 1.1:+68859527 None:intergenic
GTGACTGTGAATGAGAAGAG+TGG 0.645421 1.1:-68861265 MS.gene041779:CDS
CAAACATAGAGAATTCTACA+TGG 0.646522 1.1:-68860061 MS.gene041779:CDS
AGTTTATAAAGGGCTCACAT+TGG 0.647641 1.1:-68858288 MS.gene041779:CDS
CCCTTGGTATGATAATGGCA+AGG 0.647659 1.1:-68858572 MS.gene041779:intron
CCATTAAGCCATAAAACTAG+TGG 0.651266 1.1:+68861184 None:intergenic
ATAAAATTAAGTGGTTACCA+GGG 0.651346 1.1:-68861327 MS.gene041779:CDS
AATACCACATCACTCAGAAG+CGG 0.652328 1.1:-68859518 MS.gene041779:CDS
TAAGCAATGGAAGAACAACC+AGG 0.659633 1.1:+68861157 None:intergenic
CCTTGCCATTATCATACCAA+GGG 0.676224 1.1:+68858572 None:intergenic
GAATTGGGTCCATGACTCAG+TGG 0.681910 1.1:+68861211 None:intergenic
TATAGGAAGCATAGACAATG+GGG 0.694959 1.1:+68859107 None:intergenic
AAAAGAATAAATGCTTCACG+AGG 0.727158 1.1:+68858224 None:intergenic
CAAGCCGCTTCTGAGTGATG+TGG 0.728663 1.1:+68859514 None:intergenic
GATGTGGTATTGTTACAAGG+TGG 0.753464 1.1:+68859530 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAACTTAATATTGTAT+TGG - chr1.1:68861138-68861157 MS.gene041779:CDS 10.0%
!! GTAGTTAAATATATATTATT+AGG - chr1.1:68859194-68859213 MS.gene041779:intron 10.0%
!!! ACAATATTAAGTTTTTATAT+TGG + chr1.1:68861136-68861155 None:intergenic 10.0%
!!! GTTACTAATATTTATTATTT+TGG - chr1.1:68858618-68858637 MS.gene041779:CDS 10.0%
!! CATGTGATTAATAAATTTAT+TGG + chr1.1:68859121-68859140 None:intergenic 15.0%
!! TATTCATTCTTCTTTATATT+TGG + chr1.1:68858820-68858839 None:intergenic 15.0%
!! TTAATAAGTTCTTGATATAT+AGG + chr1.1:68860515-68860534 None:intergenic 15.0%
!!! AAAACTTATGAATTTGTTTT+GGG - chr1.1:68859229-68859248 MS.gene041779:intron 15.0%
!!! AATCCAGTTATAAATTTTAA+GGG - chr1.1:68859738-68859757 MS.gene041779:CDS 15.0%
!!! ATTAATAAATTTATTGGTCT+TGG + chr1.1:68859115-68859134 None:intergenic 15.0%
!!! ATTAATCACATGATAATTTT+TGG - chr1.1:68859129-68859148 MS.gene041779:CDS 15.0%
!!! TAAAACTTATGAATTTGTTT+TGG - chr1.1:68859228-68859247 MS.gene041779:intron 15.0%
!!! TAATCCAGTTATAAATTTTA+AGG - chr1.1:68859737-68859756 MS.gene041779:CDS 15.0%
!!! TATAAATTTTAAGGGATTTA+TGG - chr1.1:68859746-68859765 MS.gene041779:intron 15.0%
!! AAAAGAGAGATAAAATTAAG+TGG - chr1.1:68858266-68858285 MS.gene041779:CDS 20.0%
!! AATCCCTTAAAATTTATAAC+TGG + chr1.1:68859744-68859763 None:intergenic 20.0%
!! ACATGTATTTGGTATAAATT+TGG + chr1.1:68860635-68860654 None:intergenic 20.0%
!! ATTAAATTTAACCGATAAGT+GGG - chr1.1:68858862-68858881 MS.gene041779:intron 20.0%
!! GATGATTATCATGATTATAT+TGG - chr1.1:68859880-68859899 MS.gene041779:CDS 20.0%
!! TATTAAATTTAACCGATAAG+TGG - chr1.1:68858861-68858880 MS.gene041779:intron 20.0%
!!! ATTCTATCATGATTTTGAAT+TGG - chr1.1:68860418-68860437 MS.gene041779:intron 20.0%
!!! CGTATATTTTTCTAGTTAAT+TGG - chr1.1:68859498-68859517 MS.gene041779:intron 20.0%
!!! GTTATTGTCAGTTTTAATTT+TGG + chr1.1:68858938-68858957 None:intergenic 20.0%
!!! TATTCTTTTTAGATCATTCT+TGG - chr1.1:68861392-68861411 MS.gene041779:CDS 20.0%
!!! TGTTCAAATTCAACATTTTT+TGG + chr1.1:68858304-68858323 None:intergenic 20.0%
!!! TTGTTTATATTTAAAACCAG+AGG + chr1.1:68860591-68860610 None:intergenic 20.0%
!!! TTTCTGTTTGTACTTATTTT+TGG - chr1.1:68859155-68859174 MS.gene041779:intron 20.0%
! AAGTATATAGTTAAGGTTGA+TGG + chr1.1:68860568-68860587 None:intergenic 25.0%
! AATCTCATCCATTTCATATT+CGG - chr1.1:68860877-68860896 MS.gene041779:intron 25.0%
! AATTGTTAACCAGAGAAATA+AGG - chr1.1:68859707-68859726 MS.gene041779:CDS 25.0%
! ACATATAAGAAGCTAAACAT+TGG - chr1.1:68859946-68859965 MS.gene041779:intron 25.0%
! ACTTCTACTAATACATGTTA+TGG + chr1.1:68860169-68860188 None:intergenic 25.0%
! ATAAAATTAAGTGGTTACCA+GGG - chr1.1:68858275-68858294 MS.gene041779:CDS 25.0%
! ATGAGATAATGAGATTATCA+AGG + chr1.1:68861258-68861277 None:intergenic 25.0%
! ATTGTTAACCAGAGAAATAA+GGG - chr1.1:68859708-68859727 MS.gene041779:CDS 25.0%
! TAAGTGAAATTTCGTTTCAT+AGG + chr1.1:68860704-68860723 None:intergenic 25.0%
! TAGATTTAAATGATTTCCTC+AGG - chr1.1:68859660-68859679 MS.gene041779:CDS 25.0%
! TATCAAGAACTTATTAATGC+CGG - chr1.1:68860518-68860537 MS.gene041779:intron 25.0%
! TTAAATTTAACCGATAAGTG+GGG - chr1.1:68858863-68858882 MS.gene041779:intron 25.0%
! TTGCTAAAACAAAACAAATC+TGG + chr1.1:68859413-68859432 None:intergenic 25.0%
!! AATGTTTAGCTTCTTATATG+TGG + chr1.1:68859947-68859966 None:intergenic 25.0%
!! AGCTAACATAAGTTTTACAA+GGG - chr1.1:68858784-68858803 MS.gene041779:intron 25.0%
!! ATCAGTTTTCTACTTATCAA+TGG - chr1.1:68859601-68859620 MS.gene041779:CDS 25.0%
!! TATCATGATTTTGAATTGGA+AGG - chr1.1:68860422-68860441 MS.gene041779:intron 25.0%
!! TTTTCTACTTATCAATGGAA+TGG - chr1.1:68859606-68859625 MS.gene041779:CDS 25.0%
!!! ATTTTGAAAGGATTGGTTAT+AGG - chr1.1:68861225-68861244 MS.gene041779:CDS 25.0%
!!! ATTTTTGGCTTATAGTTGAT+TGG - chr1.1:68859170-68859189 MS.gene041779:intron 25.0%
AAAACTGACAATAACTGTCT+AGG - chr1.1:68858943-68858962 MS.gene041779:intron 30.0%
AAAAGAATAAATGCTTCACG+AGG + chr1.1:68861381-68861400 None:intergenic 30.0%
AAACAACACAACACAGAATA+TGG + chr1.1:68858197-68858216 None:intergenic 30.0%
AATTCTCTATGTTTGTACTG+AGG + chr1.1:68859536-68859555 None:intergenic 30.0%
ACTACAACTCGATATTCTAT+TGG - chr1.1:68860974-68860993 MS.gene041779:intron 30.0%
ATAATGAGATTATCAAGGCA+AGG + chr1.1:68861253-68861272 None:intergenic 30.0%
ATATGAAATCCCAGTTACAT+TGG + chr1.1:68860325-68860344 None:intergenic 30.0%
ATATTGGAACATTTGAGTAC+TGG - chr1.1:68859896-68859915 MS.gene041779:CDS 30.0%
CAAACATAGAGAATTCTACA+TGG - chr1.1:68859541-68859560 MS.gene041779:CDS 30.0%
CTAGTTTACCGAATATGAAA+TGG + chr1.1:68860888-68860907 None:intergenic 30.0%
GATAAAATTAAGTGGTTACC+AGG - chr1.1:68858274-68858293 MS.gene041779:CDS 30.0%
GATGGAGTCAAGTTTATAAA+GGG - chr1.1:68861304-68861323 MS.gene041779:CDS 30.0%
GTTGAATTTGAACAGTATTC+AGG - chr1.1:68858310-68858329 MS.gene041779:intron 30.0%
GTTTGGTACAAAGTCAATTA+CGG + chr1.1:68859008-68859027 None:intergenic 30.0%
TAAATTTAACCGATAAGTGG+GGG - chr1.1:68858864-68858883 MS.gene041779:intron 30.0%
TAATGAGATTATCAAGGCAA+GGG + chr1.1:68861252-68861271 None:intergenic 30.0%
TTACAATTGTTCCAAGCATA+AGG + chr1.1:68858535-68858554 None:intergenic 30.0%
TTTACTTGAATCCTTATGCT+TGG - chr1.1:68858521-68858540 MS.gene041779:intron 30.0%
! AAATTGGTCCTTTTCGTATT+AGG - chr1.1:68858482-68858501 MS.gene041779:intron 30.0%
! ACAATGCTTCTAAAAGTGAT+GGG + chr1.1:68860811-68860830 None:intergenic 30.0%
! ATATAGGAAGCATAGACAAT+GGG + chr1.1:68860499-68860518 None:intergenic 30.0%
! CTAACATAAGTTTTACAAGG+GGG - chr1.1:68858786-68858805 MS.gene041779:intron 30.0%
! GAGCTAACATAAGTTTTACA+AGG - chr1.1:68858783-68858802 MS.gene041779:intron 30.0%
! GCTAACATAAGTTTTACAAG+GGG - chr1.1:68858785-68858804 MS.gene041779:intron 30.0%
! TATATAGGAAGCATAGACAA+TGG + chr1.1:68860500-68860519 None:intergenic 30.0%
! TCATAATCGCAGATTTTGAA+AGG - chr1.1:68861213-68861232 MS.gene041779:CDS 30.0%
! TCTTCTGAACTTTCGTATTT+TGG - chr1.1:68860917-68860936 MS.gene041779:CDS 30.0%
! TGAATGAGTTGAAAGCATAT+AGG + chr1.1:68860131-68860150 None:intergenic 30.0%
! TGCTTGTATTTAAGACTAGA+GGG - chr1.1:68860661-68860680 MS.gene041779:intron 30.0%
! TTCTCTTTTGTGAAGATTTG+TGG - chr1.1:68861282-68861301 MS.gene041779:CDS 30.0%
! TTGCTTGTATTTAAGACTAG+AGG - chr1.1:68860660-68860679 MS.gene041779:intron 30.0%
! TTTTTCTATCATGTCAAAGG+CGG - chr1.1:68861082-68861101 MS.gene041779:CDS 30.0%
! TTTTTGTCCATTTACTGTGT+GGG - chr1.1:68858214-68858233 MS.gene041779:CDS 30.0%
!! AAAACTAGTGGCTTTGAATT+GGG + chr1.1:68858409-68858428 None:intergenic 30.0%
!! AAAGACGTTGTTAGTTTGTT+TGG + chr1.1:68859025-68859044 None:intergenic 30.0%
!! AACTAACAACGTCTTTTTCA+AGG - chr1.1:68859029-68859048 MS.gene041779:intron 30.0%
!! TAAAACTAGTGGCTTTGAAT+TGG + chr1.1:68858410-68858429 None:intergenic 30.0%
!! TAAAATAGTTTTGCCCATGA+AGG - chr1.1:68859373-68859392 MS.gene041779:intron 30.0%
!! TAAACATTGGCTGTGATTTT+GGG - chr1.1:68859959-68859978 MS.gene041779:intron 30.0%
!! TCTAGTCTTAAATACAAGCA+AGG + chr1.1:68860661-68860680 None:intergenic 30.0%
!!! ATTCAAAGCCACTAGTTTTA+TGG - chr1.1:68858410-68858429 MS.gene041779:intron 30.0%
!!! CGTTTTTTCTATCATGTCAA+AGG - chr1.1:68861079-68861098 MS.gene041779:CDS 30.0%
!!! TATTATTTTGGATGTTGCAG+TGG - chr1.1:68858630-68858649 MS.gene041779:CDS 30.0%
!!! TTTTTTCTTCTTCTTGACAG+TGG - chr1.1:68860446-68860465 MS.gene041779:intron 30.0%
AATGAGATTATCAAGGCAAG+GGG + chr1.1:68861251-68861270 None:intergenic 35.0%
ACAACAGATATGCAGACATT+TGG - chr1.1:68858709-68858728 MS.gene041779:intron 35.0%
AGAAATTCCCACACAGTAAA+TGG + chr1.1:68858224-68858243 None:intergenic 35.0%
AGGTCATTATGTTCTTCAGT+TGG - chr1.1:68859680-68859699 MS.gene041779:CDS 35.0%
AGTGATGTGGTATTGTTACA+AGG + chr1.1:68860078-68860097 None:intergenic 35.0%
AGTTTATAAAGGGCTCACAT+TGG - chr1.1:68861314-68861333 MS.gene041779:CDS 35.0%
ATAGAGAATTCTACATGGCT+GGG - chr1.1:68859546-68859565 MS.gene041779:CDS 35.0%
CCATTAAGCCATAAAACTAG+TGG + chr1.1:68858421-68858440 None:intergenic 35.0%
CCTTAACTATATACTTCCTC+TGG - chr1.1:68860572-68860591 MS.gene041779:intron 35.0%
GGATGGAGTCAAGTTTATAA+AGG - chr1.1:68861303-68861322 MS.gene041779:CDS 35.0%
TAACTGGGATTTCATATGCT+TGG - chr1.1:68860328-68860347 MS.gene041779:intron 35.0%
TATTCTACTATCGTTGCAAC+AGG - chr1.1:68859840-68859859 MS.gene041779:intron 35.0%
TCATATGCTCTATACATCCA+AGG + chr1.1:68860237-68860256 None:intergenic 35.0%
TGACACTCGTGATTAAGAAA+TGG + chr1.1:68860835-68860854 None:intergenic 35.0%
TTACTGTGTGGGAATTTCTT+TGG - chr1.1:68858225-68858244 MS.gene041779:CDS 35.0%
TTATGAAGCACAGACAATGA+CGG - chr1.1:68858761-68858780 MS.gene041779:intron 35.0%
! CACAATGCTTCTAAAAGTGA+TGG + chr1.1:68860812-68860831 None:intergenic 35.0%
! CATAATAAGTGTCACGCTTT+TGG + chr1.1:68861113-68861132 None:intergenic 35.0%
! CCAGAGGAAGTATATAGTTA+AGG + chr1.1:68860575-68860594 None:intergenic 35.0%
! CTTTTGTGAAGATTTGTGGA+TGG - chr1.1:68861286-68861305 MS.gene041779:CDS 35.0%
! GTTTTTGTCCATTTACTGTG+TGG - chr1.1:68858213-68858232 MS.gene041779:CDS 35.0%
! TACAGATTCCCTTATTTCTC+TGG + chr1.1:68859719-68859738 None:intergenic 35.0%
! TATAGGAAGCATAGACAATG+GGG + chr1.1:68860498-68860517 None:intergenic 35.0%
! TATAGTTTTCCCCCACTTAT+CGG + chr1.1:68858876-68858895 None:intergenic 35.0%
! TATGGTGCTTCTGAAGAAAT+TGG - chr1.1:68858466-68858485 MS.gene041779:intron 35.0%
!! ATCGCAGATTTTGAAAGGAT+TGG - chr1.1:68861218-68861237 MS.gene041779:CDS 35.0%
!! CTAAACATTGGCTGTGATTT+TGG - chr1.1:68859958-68859977 MS.gene041779:intron 35.0%
!!! CCACTAGTTTTATGGCTTAA+TGG - chr1.1:68858418-68858437 MS.gene041779:intron 35.0%
!!! CTAGTTTTATGGCTTAATGG+CGG - chr1.1:68858421-68858440 MS.gene041779:intron 35.0%
!!! TTTTTTTGACTTTGCAGCTG+AGG - chr1.1:68859472-68859491 MS.gene041779:intron 35.0%
AACTTATTAATGCCGGTCTC+CGG - chr1.1:68860525-68860544 MS.gene041779:intron 40.0%
AATACCACATCACTCAGAAG+CGG - chr1.1:68860084-68860103 MS.gene041779:CDS 40.0%
ACAGTTGCAATTACTGAGCA+AGG - chr1.1:68860021-68860040 MS.gene041779:intron 40.0%
ACCTTGCCATTATCATACCA+AGG + chr1.1:68861034-68861053 None:intergenic 40.0%
ACTGAGCAAGGAAACATTGA+TGG - chr1.1:68860033-68860052 MS.gene041779:intron 40.0%
ACTGCGAGTAAATCCTTCAT+GGG + chr1.1:68859389-68859408 None:intergenic 40.0%
AGCTACCAACCATCATCAAT+TGG - chr1.1:68861005-68861024 MS.gene041779:intron 40.0%
ATTCGAGCCATTATCAGTGT+GGG - chr1.1:68861170-68861189 MS.gene041779:CDS 40.0%
CACTTCATGCCAATGTAACT+GGG - chr1.1:68860313-68860332 MS.gene041779:intron 40.0%
CATAGAGAATTCTACATGGC+TGG - chr1.1:68859545-68859564 MS.gene041779:CDS 40.0%
CATATGATCCATGTGCTGAA+AGG - chr1.1:68860250-68860269 MS.gene041779:intron 40.0%
CCTTGCCATTATCATACCAA+GGG + chr1.1:68861033-68861052 None:intergenic 40.0%
CTGAAGAACATAATGACCTG+AGG + chr1.1:68859679-68859698 None:intergenic 40.0%
GATGTGGTATTGTTACAAGG+TGG + chr1.1:68860075-68860094 None:intergenic 40.0%
GGTTGTTCTTCCATTGCTTA+TGG - chr1.1:68858448-68858467 MS.gene041779:intron 40.0%
TAAGAAAAACCAACACCAGC+AGG + chr1.1:68858679-68858698 None:intergenic 40.0%
TAAGCAATGGAAGAACAACC+AGG + chr1.1:68858448-68858467 None:intergenic 40.0%
TAGAGAATTCTACATGGCTG+GGG - chr1.1:68859547-68859566 MS.gene041779:CDS 40.0%
TCAGAATACCTTTCAGCACA+TGG + chr1.1:68860261-68860280 None:intergenic 40.0%
TCTTGACAGTGGTACTGTTT+GGG - chr1.1:68860457-68860476 MS.gene041779:intron 40.0%
TGGGATTTCATATGCTTGGA+AGG - chr1.1:68860332-68860351 MS.gene041779:intron 40.0%
TGGTACCCTTGGTATGATAA+TGG - chr1.1:68861025-68861044 MS.gene041779:intron 40.0%
TTCAAGTCCCACACTGATAA+TGG + chr1.1:68861180-68861199 None:intergenic 40.0%
TTCTTGACAGTGGTACTGTT+TGG - chr1.1:68860456-68860475 MS.gene041779:intron 40.0%
! AAGTAAAGTGACTTGCCATC+AGG + chr1.1:68858508-68858527 None:intergenic 40.0%
! ACATTTGGTGACCAGAAAAC+AGG - chr1.1:68858724-68858743 MS.gene041779:intron 40.0%
! AGTTTTACAAGGGGGTCAAT+TGG - chr1.1:68858794-68858813 MS.gene041779:intron 40.0%
! CGACTACATTACCTGTTTTC+TGG + chr1.1:68858738-68858757 None:intergenic 40.0%
! GAATATCGAGTTGTAGTCAG+TGG + chr1.1:68860971-68860990 None:intergenic 40.0%
! GTGGTACTGTTTGGGATTAT+TGG - chr1.1:68860465-68860484 MS.gene041779:intron 40.0%
! TGGTCTTGGTTAGACACTTT+CGG + chr1.1:68859101-68859120 None:intergenic 40.0%
!! TGAACAAATCGAAGCATCGA+GGG + chr1.1:68858585-68858604 None:intergenic 40.0%
!! TGGTATGATAATGGCAAGGT+TGG - chr1.1:68861034-68861053 MS.gene041779:intron 40.0%
!! TTCAGAAGCACCATAAGCAA+TGG + chr1.1:68858461-68858480 None:intergenic 40.0%
!! TTGAACAAATCGAAGCATCG+AGG + chr1.1:68858586-68858605 None:intergenic 40.0%
!! TTGGAACATTTGAGTACTGG+TGG - chr1.1:68859899-68859918 MS.gene041779:CDS 40.0%
!!! GAATTTGTTTTGGGCAGTCA+TGG - chr1.1:68859238-68859257 MS.gene041779:intron 40.0%
!!! GTGGAAGAGCATTGTTTTAC+TGG - chr1.1:68858356-68858375 MS.gene041779:intron 40.0%
!!! TCAACATTTTTTGGTTGCCC+TGG + chr1.1:68858295-68858314 None:intergenic 40.0%
AAGCAAGGCGCACATGTATT+TGG + chr1.1:68860646-68860665 None:intergenic 45.0%
AAGTGGGGGAAAACTATACG+CGG - chr1.1:68858878-68858897 MS.gene041779:intron 45.0%
ATACATCCAAGGCTGAAGAG+AGG + chr1.1:68860226-68860245 None:intergenic 45.0%
CACTGCGAGTAAATCCTTCA+TGG + chr1.1:68859390-68859409 None:intergenic 45.0%
CATTCGAGCCATTATCAGTG+TGG - chr1.1:68861169-68861188 MS.gene041779:CDS 45.0%
CATTCGTCCAGAAGTTCAGA+AGG - chr1.1:68860290-68860309 MS.gene041779:intron 45.0%
CCATCAGGCCTAATACGAAA+AGG + chr1.1:68858493-68858512 None:intergenic 45.0%
CCATCATCAATTGGTACCCT+TGG - chr1.1:68861014-68861033 MS.gene041779:intron 45.0%
CTACTATCGTTGCAACAGGT+TGG - chr1.1:68859844-68859863 MS.gene041779:intron 45.0%
CTATCATGTCAAAGGCGGTA+AGG - chr1.1:68861087-68861106 MS.gene041779:CDS 45.0%
CTCACATTGGTCACAGTAAG+AGG - chr1.1:68861327-68861346 MS.gene041779:CDS 45.0%
GCACTTCATGCCAATGTAAC+TGG - chr1.1:68860312-68860331 MS.gene041779:intron 45.0%
GTGACTGTGAATGAGAAGAG+TGG - chr1.1:68858337-68858356 MS.gene041779:intron 45.0%
TACATCCAAGGCTGAAGAGA+GGG + chr1.1:68860225-68860244 None:intergenic 45.0%
TCCATCAGTGCAATGTTTGC+AGG - chr1.1:68859993-68860012 MS.gene041779:intron 45.0%
! CCTTTTCGTATTAGGCCTGA+TGG - chr1.1:68858490-68858509 MS.gene041779:intron 45.0%
! CGGTGTCTATTTTGTGCTCA+GGG + chr1.1:68859270-68859289 None:intergenic 45.0%
! GGTGTCTATTTTGTGCTCAG+GGG + chr1.1:68859269-68859288 None:intergenic 45.0%
! TGAAGTGCCTTCTGAACTTC+TGG + chr1.1:68860300-68860319 None:intergenic 45.0%
! TTGCACTGATGGATGCTGAA+TGG + chr1.1:68859986-68860005 None:intergenic 45.0%
! TTTTTCCCTCTCTTCAGCCT+TGG - chr1.1:68860217-68860236 MS.gene041779:intron 45.0%
!! AGTACTAGTGTTGACGTGTC+TGG + chr1.1:68859302-68859321 None:intergenic 45.0%
!! CACACTGATAATGGCTCGAA+TGG + chr1.1:68861171-68861190 None:intergenic 45.0%
!! CCAAGGGTACCAATTGATGA+TGG + chr1.1:68861017-68861036 None:intergenic 45.0%
!! CCCTTGGTATGATAATGGCA+AGG - chr1.1:68861030-68861049 MS.gene041779:intron 45.0%
!! GATGATGGTTGGTAGCTTCA+AGG + chr1.1:68861002-68861021 None:intergenic 45.0%
!! GGGTACCAATTGATGATGGT+TGG + chr1.1:68861013-68861032 None:intergenic 45.0%
!! TCGGTGTCTATTTTGTGCTC+AGG + chr1.1:68859271-68859290 None:intergenic 45.0%
!! TTTGAGTACTGGTGGACACA+TGG - chr1.1:68859907-68859926 MS.gene041779:CDS 45.0%
!!! CTTTCGTATTTTGGCTGCAG+TGG - chr1.1:68860926-68860945 MS.gene041779:CDS 45.0%
ATGGCTGGGGAAAGTTATGC+AGG - chr1.1:68859560-68859579 MS.gene041779:CDS 50.0%
GAATTGGGTCCATGACTCAG+TGG + chr1.1:68858394-68858413 None:intergenic 50.0%
GCCTGCAAACATTGCACTGA+TGG + chr1.1:68859997-68860016 None:intergenic 50.0%
GCTTCACGAGGCTTATGAAG+AGG + chr1.1:68861369-68861388 None:intergenic 50.0%
GGTACCTGTATACCCATGTC+CGG + chr1.1:68860547-68860566 None:intergenic 50.0%
TTATGGCTTAATGGCGGTCC+TGG - chr1.1:68858427-68858446 MS.gene041779:intron 50.0%
! ACTGAAGCACCACTGAGTCA+TGG - chr1.1:68858382-68858401 MS.gene041779:intron 50.0%
! CATATGCTTGGAAGGCATGC+AGG - chr1.1:68860340-68860359 MS.gene041779:intron 50.0%
! GACGTGTCTGGTGTCAATGT+CGG + chr1.1:68859290-68859309 None:intergenic 50.0%
! TTGGTCACAGTAAGAGGTGC+TGG - chr1.1:68861333-68861352 MS.gene041779:CDS 50.0%
!! AGTAAGAGGTGCTGGACATG+AGG - chr1.1:68861341-68861360 MS.gene041779:CDS 50.0%
CAACACCAGCAGGAGACTCA+AGG + chr1.1:68858669-68858688 None:intergenic 55.0%
CAAGCCGCTTCTGAGTGATG+TGG + chr1.1:68860091-68860110 None:intergenic 55.0%
CACATGTGTCAGCGTTGCGT+CGG - chr1.1:68859335-68859354 MS.gene041779:CDS 55.0%
CTGTTCCTTGAGTCTCCTGC+TGG - chr1.1:68858661-68858680 MS.gene041779:intron 55.0%
CTTGAGTCTCCTGCTGGTGT+TGG - chr1.1:68858667-68858686 MS.gene041779:intron 55.0%
GTATACCCATGTCCGGAGAC+CGG + chr1.1:68860540-68860559 None:intergenic 55.0%
GTCTCCGGACATGGGTATAC+AGG - chr1.1:68860540-68860559 MS.gene041779:intron 55.0%
TAATGCCGGTCTCCGGACAT+GGG - chr1.1:68860532-68860551 MS.gene041779:intron 55.0%
TTAATGCCGGTCTCCGGACA+TGG - chr1.1:68860531-68860550 MS.gene041779:intron 55.0%
CGACGCAACGCTGACACATG+TGG + chr1.1:68859337-68859356 None:intergenic 60.0%
! GCGTTGCGTCGGTGATGCAT+AGG - chr1.1:68859346-68859365 MS.gene041779:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 68858184 68861440 68858184 ID=MS.gene041779
chr1.1 mRNA 68858184 68861440 68858184 ID=MS.gene041779.t1;Parent=MS.gene041779
chr1.1 exon 68861074 68861440 68861074 ID=MS.gene041779.t1.exon1;Parent=MS.gene041779.t1
chr1.1 CDS 68861074 68861440 68861074 ID=cds.MS.gene041779.t1;Parent=MS.gene041779.t1
chr1.1 exon 68860879 68860974 68860879 ID=MS.gene041779.t1.exon2;Parent=MS.gene041779.t1
chr1.1 CDS 68860879 68860974 68860879 ID=cds.MS.gene041779.t1;Parent=MS.gene041779.t1
chr1.1 exon 68860043 68860135 68860043 ID=MS.gene041779.t1.exon3;Parent=MS.gene041779.t1
chr1.1 CDS 68860043 68860135 68860043 ID=cds.MS.gene041779.t1;Parent=MS.gene041779.t1
chr1.1 exon 68859857 68859942 68859857 ID=MS.gene041779.t1.exon4;Parent=MS.gene041779.t1
chr1.1 CDS 68859857 68859942 68859857 ID=cds.MS.gene041779.t1;Parent=MS.gene041779.t1
chr1.1 exon 68859502 68859762 68859502 ID=MS.gene041779.t1.exon5;Parent=MS.gene041779.t1
chr1.1 CDS 68859502 68859762 68859502 ID=cds.MS.gene041779.t1;Parent=MS.gene041779.t1
chr1.1 exon 68859263 68859390 68859263 ID=MS.gene041779.t1.exon6;Parent=MS.gene041779.t1
chr1.1 CDS 68859263 68859390 68859263 ID=cds.MS.gene041779.t1;Parent=MS.gene041779.t1
chr1.1 exon 68859063 68859158 68859063 ID=MS.gene041779.t1.exon7;Parent=MS.gene041779.t1
chr1.1 CDS 68859063 68859158 68859063 ID=cds.MS.gene041779.t1;Parent=MS.gene041779.t1
chr1.1 exon 68858573 68858678 68858573 ID=MS.gene041779.t1.exon8;Parent=MS.gene041779.t1
chr1.1 CDS 68858573 68858678 68858573 ID=cds.MS.gene041779.t1;Parent=MS.gene041779.t1
chr1.1 exon 68858184 68858327 68858184 ID=MS.gene041779.t1.exon9;Parent=MS.gene041779.t1
chr1.1 CDS 68858184 68858327 68858184 ID=cds.MS.gene041779.t1;Parent=MS.gene041779.t1
Gene Sequence

>MS.gene041779

ATGAGTCATTCCATATTCTGTGTTGTGTTGTTTTTGTCCATTTACTGTGTGGGAATTTCTTTGGCTTCTTCTATAAAAGAACAAAAGAGAGATAAAATTAAGTGGTTACCAGGGCAACCAAAAAATGTTGAATTTGAACAGTATTCAGGTTATGTGACTGTGAATGAGAAGAGTGGAAGAGCATTGTTTTACTGGTTGACTGAAGCACCACTGAGTCATGGACCCAATTCAAAGCCACTAGTTTTATGGCTTAATGGCGGTCCTGGTTGTTCTTCCATTGCTTATGGTGCTTCTGAAGAAATTGGTCCTTTTCGTATTAGGCCTGATGGCAAGTCACTTTACTTGAATCCTTATGCTTGGAACAATTGTAAGTCGAATTCTCATCTAATGAAATTCAACCCTCGATGCTTCGATTTGTTCAACAAAAAAATATCGTTACTAATATTTATTATTTTGGATGTTGCAGTGGCAAACATACTGTTCCTTGAGTCTCCTGCTGGTGTTGGTTTTTCTTATTGTAATGAAACAACAGATATGCAGACATTTGGTGACCAGAAAACAGGTAATGTAGTCGTAATTATGAAGCACAGACAATGACGGAGCTAACATAAGTTTTACAAGGGGGTCAATTGGCCAAATATAAAGAAGAATGAATAAACTACATTTAAAAACTAATATATTAAATTTAACCGATAAGTGGGGGAAAACTATACGCGGAAAATTTCAGAAGAATTTTTTTCTTATATAAAATCCAAAATTAAAACTGACAATAACTGTCTAGGAAAGTTGAGCTTGACAACACGTCTAACAGTCAAATTCATCCGTAATTGACTTTGTACCAAACAAACTAACAACGTCTTTTTCAAGGTAAATAGCTGATCTATTCGCAAGAAACACTTTAATTGCTCAAGAGTCCGAAAGTGTCTAACCAAGACCAATAAATTTATTAATCACATGATAATTTTTGGACTTTTCTGTTTGTACTTATTTTTGGCTTATAGTTGATTGGAGTAGTTAAATATATATTATTAGGCTAGATAAATATAAAACTTATGAATTTGTTTTGGGCAGTCATGGCTACGCCCCTGAGCACAAAATAGACACCGACATTGACACCAGACACGTCAACACTAGTACTAACTGAATGTAGCCACATGTGTCAGCGTTGCGTCGGTGATGCATAGGTTTGTAAAATAGTTTTGCCCATGAAGGATTTACTCGCAGTGCCAGATTTGTTTTGTTTTAGCAATGAGATTTGACGTGTTGATATGTATCTGCGATCTGTAACTTTTTTTGACTTTGCAGCTGAGGATGCGTATATTTTTCTAGTTAATTGGTTTGAAAGATTTCCTCAGTACAAACATAGAGAATTCTACATGGCTGGGGAAAGTTATGCAGGTGAATCTATATTTGATATATCAGTTTTCTACTTATCAATGGAATGGCTTGTACAATGAAGAATTGATCAATTTCTTATAGATTTAAATGATTTCCTCAGGTCATTATGTTCTTCAGTTGGCTCAAATTGTTAACCAGAGAAATAAGGGAATCTGTAATCCAGTTATAAATTTTAAGGGATTTATGGTAAGCTTTTTAGATTCATGTTTAAATCTTTTATGTTCACATAGACAGAACAAAACTAACTTATGATAATCATATTCTACTATCGTTGCAACAGGTTGGAAATAGCGTGATTGATGATTATCATGATTATATTGGAACATTTGAGTACTGGTGGACACATGGTTTGATTTCAGATTCCACATATAAGAAGCTAAACATTGGCTGTGATTTTGGGTCCATTCAGCATCCATCAGTGCAATGTTTGCAGGCTCTTACAGTTGCAATTACTGAGCAAGGAAACATTGATGGATATAGCATTAATACACCACCTTGTAACAATACCACATCACTCAGAAGCGGCTTGCATGATCGTTATGTAAGCCTATATGCTTTCAACTCATTCACATTGCGTACAAAAACCATAACATGTATTAGTAGAAGTGTTTTGATGATTTTTTTTTCTTGTTTCTTTTTTCCCTCTCTTCAGCCTTGGATGTATAGAGCATATGATCCATGTGCTGAAAGGTATTCTGATGTGTACTTCATTCGTCCAGAAGTTCAGAAGGCACTTCATGCCAATGTAACTGGGATTTCATATGCTTGGAAGGCATGCAGGTATATTAATTTTAATTTAGTAAATTGATATGTAACATGTTCTATTGAAGTCTATAATTCTATCATGATTTTGAATTGGAAGGTTTTTTTCTTCTTCTTGACAGTGGTACTGTTTGGGATTATTGGACTGATTCCCCATTGTCTATGCTTCCTATATATCAAGAACTTATTAATGCCGGTCTCCGGACATGGGTATACAGGTACCATCAACCTTAACTATATACTTCCTCTGGTTTTAAATATAAACAAAAAGTTACATTTATATCTGATCCAAATTTATACCAAATACATGTGCGCCTTGCTTGTATTTAAGACTAGAGGGAGTATTTGCGTTTGACTCCTATGAAACGAAATTTCACTTAAAAGTATGAAGCAAGATATATATCTCAACACTTGTCAAAATAATTCATATGATCTAATGTGAATTACTTATTTAAGAGTCTAATCCCATCACTTTTAGAAGCATTGTGCCATTTCTTAATCACGAGTGTCACATATCATGTTTTTATTTCAGCAATCTCATCCATTTCATATTCGGTAAACTAGAACTCGAATTCTTCTGAACTTTCGTATTTTGGCTGCAGTGGAGACACTGATGCTGTAATTCCACTGACTACAACTCGATATTCTATTGGTGCCTTGAAGCTACCAACCATCATCAATTGGTACCCTTGGTATGATAATGGCAAGGTTGGTGATAACTTCATTCTATCGTGTCGTTTTTTCTATCATGTCAAAGGCGGTAAGGCCAAAAGCGTGACACTTATTATGCCAATATAAAAACTTAATATTGTATTGGAGCTTATCCATTCGAGCCATTATCAGTGTGGGACTTGAATAATCTCATTATCTCATAATCGCAGATTTTGAAAGGATTGGTTATAGGCCCCTTGCCTTGATAATCTCATTATCTCATAATTTTCTCTTTTGTGAAGATTTGTGGATGGAGTCAAGTTTATAAAGGGCTCACATTGGTCACAGTAAGAGGTGCTGGACATGAGGTTCCTCTTCATAAGCCTCGTGAAGCATTTATTCTTTTTAGATCATTCTTGGAAAACAAGAATATGCCATCAAGTTAA
Protein sequence

>MS.gene041779.t1

MSHSIFCVVLFLSIYCVGISLASSIKEQKRDKIKWLPGQPKNVEFEQYSGYVTVNEKSGRALFYWLTEAPLSHGPNSKPLVLWLNGGPGCSSIAYGASEEIGPFRIRPDGKSLYLNPYAWNNLANILFLESPAGVGFSYCNETTDMQTFGDQKTAEDAYIFLVNWFERFPQYKHREFYMAGESYAGHYVLQLAQIVNQRNKGICNPVINFKGFMVGNSVIDDYHDYIGTFEYWWTHGLISDSTYKKLNIGCDFGSIQHPSVQCLQALTVAITEQGNIDGYSINTPPCNNTTSLRSGLHDRYPWMYRAYDPCAERYSDVYFIRPEVQKALHANVTGISYAWKACSGTVWDYWTDSPLSMLPIYQELINAGLRTWVYSGDTDAVIPLTTTRYSIGALKLPTIINWYPWYDNGKICGWSQVYKGLTLVTVRGAGHEVPLHKPREAFILFRSFLENKNMPSS