AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene032088

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032088.t1 MTR_2g081620 94.118 476 26 1 1 476 1 474 0.0 935
MS.gene032088.t1 MTR_2g063000 83.191 470 73 2 1 468 1 466 0.0 827
MS.gene032088.t1 MTR_2g062980 80.421 475 90 1 1 475 1 472 0.0 813
MS.gene032088.t1 MTR_2g062990 79.661 472 93 1 1 472 1 469 0.0 807
MS.gene032088.t1 MTR_2g063000 77.021 470 71 3 1 468 1 435 0.0 750
MS.gene032088.t1 MTR_1g095560 54.405 454 202 2 14 466 8 457 0.0 532
MS.gene032088.t1 MTR_1g095560 50.823 486 202 3 14 466 8 489 0.0 516
MS.gene032088.t1 MTR_1g095540 53.304 454 209 2 15 466 5 457 6.32e-179 509
MS.gene032088.t1 MTR_7g013790 49.679 467 219 9 12 467 5 466 1.71e-167 480
MS.gene032088.t1 MTR_3g079570 49.765 426 202 6 40 465 77 490 1.13e-149 436
MS.gene032088.t1 MTR_3g079590 50.000 422 200 6 40 461 76 486 6.13e-147 429
MS.gene032088.t1 MTR_3g079560 48.019 429 212 5 38 466 74 491 5.82e-146 427
MS.gene032088.t1 MTR_3g079600 48.174 438 212 7 37 471 71 496 4.07e-143 419
MS.gene032088.t1 MTR_1g020090 48.140 430 206 7 39 467 86 499 1.29e-140 413
MS.gene032088.t1 MTR_3g079620 46.560 436 212 7 40 466 76 499 4.11e-140 412
MS.gene032088.t1 MTR_2g012860 43.882 474 244 7 14 468 6 476 8.53e-140 410
MS.gene032088.t1 MTR_3g034290 45.067 446 230 8 29 468 57 493 2.26e-139 410
MS.gene032088.t1 MTR_1g021010 48.598 428 204 7 43 468 76 489 5.22e-138 406
MS.gene032088.t1 MTR_3g079630 47.806 433 201 8 40 461 76 494 9.03e-138 406
MS.gene032088.t1 MTR_4g122950 43.552 473 236 8 22 466 9 478 2.66e-136 402
MS.gene032088.t1 MTR_4g075150 45.336 461 232 11 15 465 11 461 3.39e-136 400
MS.gene032088.t1 MTR_4g075150 45.140 463 232 11 15 465 11 463 1.17e-135 399
MS.gene032088.t1 MTR_1g021020 46.897 435 216 7 35 468 70 490 1.46e-134 397
MS.gene032088.t1 MTR_4g089155 45.330 439 225 7 42 468 53 488 2.27e-134 397
MS.gene032088.t1 MTR_0003s0560 46.101 436 223 8 37 471 69 493 6.91e-133 393
MS.gene032088.t1 MTR_2g012850 43.103 464 250 8 14 466 6 466 1.70e-132 391
MS.gene032088.t1 MTR_4g122960 42.887 471 240 8 22 466 9 476 2.15e-129 384
MS.gene032088.t1 MTR_3g090130 40.136 441 253 8 40 472 26 463 1.28e-112 340
MS.gene032088.t1 MTR_5g072570 39.076 476 265 11 11 473 6 469 5.75e-112 339
MS.gene032088.t1 MTR_3g034290 45.429 361 183 7 29 384 57 408 1.36e-110 333
MS.gene032088.t1 MTR_2g449850 39.640 444 240 10 37 466 34 463 5.10e-107 326
MS.gene032088.t1 MTR_3g090150 38.269 439 260 8 43 472 34 470 7.67e-106 323
MS.gene032088.t1 MTR_3g434850 37.716 464 265 11 16 467 5 456 1.05e-103 317
MS.gene032088.t1 MTR_8g099930 38.530 449 245 14 35 465 27 462 3.50e-102 313
MS.gene032088.t1 MTR_3g498755 38.652 445 246 13 40 467 13 447 1.88e-100 308
MS.gene032088.t1 MTR_1g040370 37.875 433 220 11 38 465 27 415 3.41e-99 304
MS.gene032088.t1 MTR_7g021130 36.596 470 264 13 17 465 1 457 1.24e-98 304
MS.gene032088.t1 MTR_8g011350 32.797 497 279 10 16 464 6 495 7.73e-86 272
MS.gene032088.t1 MTR_0003s0560 49.434 265 128 6 37 301 69 327 1.27e-84 265
MS.gene032088.t1 MTR_7g080180 33.663 505 267 18 17 464 5 498 5.33e-81 260
MS.gene032088.t1 MTR_7g051430 32.049 493 277 13 19 461 8 492 2.78e-77 250
MS.gene032088.t1 MTR_8g011350 31.702 470 266 10 16 437 6 468 2.64e-74 242
MS.gene032088.t1 MTR_7g080180 33.261 463 242 17 17 423 5 456 1.16e-69 229
MS.gene032088.t1 MTR_1g102130 31.897 464 264 14 17 465 64 490 1.17e-69 230
MS.gene032088.t1 MTR_7g051430 39.446 289 164 7 19 302 8 290 7.18e-59 199
MS.gene032088.t1 MTR_4g021535 29.374 463 294 15 21 460 10 462 4.46e-56 193
MS.gene032088.t1 MTR_4g021510 30.184 434 270 13 43 455 39 460 1.09e-54 190
MS.gene032088.t1 MTR_2g018590 28.421 475 306 13 11 464 8 469 4.71e-54 188
MS.gene032088.t1 MTR_2g018630 29.613 439 276 13 42 460 36 461 1.22e-53 187
MS.gene032088.t1 MTR_2g018600 30.464 453 269 16 42 464 33 469 2.99e-53 186
MS.gene032088.t1 MTR_4g021530 28.541 466 294 16 21 460 14 466 3.15e-53 186
MS.gene032088.t1 MTR_3g100420 29.218 486 308 14 7 464 19 496 4.05e-53 186
MS.gene032088.t1 MTR_4g021520 29.705 441 273 15 43 460 40 466 1.13e-52 184
MS.gene032088.t1 MTR_4g021500 30.734 436 271 15 43 458 40 464 1.44e-51 187
MS.gene032088.t1 MTR_4g021500 29.108 426 271 14 55 460 487 901 2.38e-48 178
MS.gene032088.t1 MTR_4g021500 30.734 436 271 15 43 458 40 464 1.77e-51 187
MS.gene032088.t1 MTR_4g021500 29.665 418 261 14 55 460 487 883 2.60e-48 178
MS.gene032088.t1 MTR_2g095730 29.286 420 263 9 57 461 94 494 2.17e-51 182
MS.gene032088.t1 MTR_2g095750 30.505 436 244 13 57 466 100 502 1.85e-50 179
MS.gene032088.t1 MTR_4g021490 28.798 441 277 16 43 460 40 466 1.18e-49 176
MS.gene032088.t1 MTR_1g050545 30.476 420 261 11 55 462 81 481 2.72e-46 168
MS.gene032088.t1 MTR_4g057570 28.251 446 268 12 40 461 72 489 1.01e-45 166
MS.gene032088.t1 MTR_4g057585 30.048 416 261 11 57 461 96 492 4.87e-45 164
MS.gene032088.t1 MTR_4g057595 30.695 417 257 12 57 461 97 493 1.11e-44 164
MS.gene032088.t1 MTR_4g021520 29.777 403 247 14 43 423 40 428 2.78e-44 161
MS.gene032088.t1 MTR_1g094155 27.824 478 296 16 12 462 32 487 1.20e-43 160
MS.gene032088.t1 MTR_3g045770 26.437 435 294 11 42 460 26 450 1.96e-43 159
MS.gene032088.t1 MTR_4g057600 26.977 430 262 12 54 461 92 491 4.80e-40 150
MS.gene032088.t1 MTR_5g097180 26.856 458 283 16 20 459 9 432 3.68e-39 147
MS.gene032088.t1 MTR_4g021535 25.702 463 264 16 21 460 10 415 8.18e-39 146
MS.gene032088.t1 MTR_2g018630 27.684 354 230 10 122 460 14 356 1.02e-36 139
MS.gene032088.t1 MTR_0075s0010 25.737 373 204 12 39 404 5 311 4.72e-34 130
MS.gene032088.t1 MTR_4g084710 25.899 417 266 13 59 459 45 434 6.89e-34 133
MS.gene032088.t1 MTR_2g095710 26.077 418 265 12 57 461 94 480 1.97e-33 132
MS.gene032088.t1 MTR_2g009200 26.269 453 268 14 35 459 20 434 6.70e-33 130
MS.gene032088.t1 MTR_1g051175 37.391 115 65 4 52 165 21 129 1.12e-17 83.6
MS.gene032088.t1 MTR_4g056520 21.839 435 288 14 60 459 36 453 5.41e-17 83.6
MS.gene032088.t1 MTR_7g100560 27.907 215 87 6 52 266 67 213 4.74e-16 79.3
MS.gene032088.t1 MTR_2g084530 23.776 429 275 15 60 454 36 446 7.39e-13 70.9
MS.gene032088.t1 MTR_4g122970 43.182 88 39 3 13 91 2 87 1.70e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032088.t1 AT4G30810 61.378 479 176 4 1 476 1 473 0.0 616
MS.gene032088.t1 AT2G35780 55.329 441 187 5 29 465 17 451 1.69e-179 510
MS.gene032088.t1 AT3G07990 55.220 431 191 2 36 465 28 457 1.32e-178 508
MS.gene032088.t1 AT4G30610 51.634 459 206 7 20 465 7 462 6.84e-165 474
MS.gene032088.t1 AT3G02110 50.112 445 202 7 36 465 32 471 2.19e-161 465
MS.gene032088.t1 AT2G24010 50.000 432 212 4 35 465 23 451 1.63e-156 452
MS.gene032088.t1 AT2G24000 49.441 447 213 6 28 464 17 460 6.10e-154 446
MS.gene032088.t1 AT2G24010 48.098 447 213 5 35 465 23 466 4.34e-151 439
MS.gene032088.t1 AT2G35770 47.912 455 220 8 21 466 13 459 1.56e-147 429
MS.gene032088.t1 AT2G24010 46.237 465 206 6 42 465 1 462 3.80e-144 421
MS.gene032088.t1 AT1G61130 45.000 460 231 10 19 465 10 460 2.13e-134 396
MS.gene032088.t1 AT5G23210 45.614 456 218 8 37 467 47 497 1.37e-133 395
MS.gene032088.t1 AT3G63470 44.496 427 224 7 42 465 81 497 3.92e-133 394
MS.gene032088.t1 AT1G11080 42.171 479 254 7 7 465 14 489 4.98e-132 391
MS.gene032088.t1 AT1G11080 42.171 479 254 7 7 465 14 489 5.16e-132 391
MS.gene032088.t1 AT5G08260 42.384 453 241 8 31 466 28 477 1.45e-129 384
MS.gene032088.t1 AT3G17180 42.729 447 235 8 37 466 34 476 6.91e-125 372
MS.gene032088.t1 AT4G15100 41.850 454 243 7 35 471 38 487 2.98e-124 371
MS.gene032088.t1 AT3G52020 41.743 436 240 10 36 465 71 498 1.94e-118 357
MS.gene032088.t1 AT1G43780 38.763 485 276 11 1 472 1 477 2.73e-115 348
MS.gene032088.t1 AT5G23210 44.634 410 197 8 37 421 47 451 9.25e-114 343
MS.gene032088.t1 AT2G12480 39.910 446 253 9 39 476 28 466 9.63e-113 341
MS.gene032088.t1 AT2G24010 47.632 359 169 5 42 384 1 356 4.43e-112 335
MS.gene032088.t1 AT5G23210 44.361 399 195 5 94 467 6 402 4.58e-112 337
MS.gene032088.t1 AT5G23210 44.192 396 194 5 97 467 8 401 4.17e-111 334
MS.gene032088.t1 AT3G52000 40.411 438 243 12 34 465 54 479 1.41e-110 336
MS.gene032088.t1 AT5G42240 40.547 439 246 8 39 465 29 464 4.36e-110 334
MS.gene032088.t1 AT2G05850 39.359 437 246 11 37 465 59 484 9.95e-108 328
MS.gene032088.t1 AT3G17180 40.898 401 217 7 37 421 34 430 3.35e-105 320
MS.gene032088.t1 AT2G33530 40.449 445 244 12 34 465 26 462 4.76e-104 318
MS.gene032088.t1 AT3G52010 38.952 439 244 14 37 465 60 484 2.52e-103 317
MS.gene032088.t1 AT5G42230 37.358 439 254 9 42 465 28 460 2.49e-102 314
MS.gene032088.t1 AT5G42230 37.358 439 254 9 42 465 28 460 2.49e-102 314
MS.gene032088.t1 AT2G12480 37.753 445 240 9 39 476 28 442 3.38e-102 313
MS.gene032088.t1 AT2G12480 37.528 445 239 10 39 476 28 440 1.46e-100 308
MS.gene032088.t1 AT2G12480 37.444 446 239 11 39 476 28 441 9.85e-99 304
MS.gene032088.t1 AT1G28110 38.864 440 244 12 40 465 30 458 1.11e-98 304
MS.gene032088.t1 AT1G28110 38.864 440 244 12 40 465 30 458 1.11e-98 304
MS.gene032088.t1 AT5G23210 42.857 350 173 5 97 421 8 355 3.41e-91 282
MS.gene032088.t1 AT4G12910 33.133 498 280 10 14 464 6 497 4.97e-90 283
MS.gene032088.t1 AT3G25420 31.590 497 285 11 17 464 4 494 3.00e-85 271
MS.gene032088.t1 AT4G12910 32.237 456 257 9 14 423 6 455 2.14e-76 246
MS.gene032088.t1 AT2G24000 43.929 280 146 4 195 464 119 397 1.01e-74 241
MS.gene032088.t1 AT2G24000 57.282 103 42 2 28 130 17 117 7.92e-31 123
MS.gene032088.t1 AT3G56540 50.794 189 87 4 13 198 47 232 2.87e-63 206
MS.gene032088.t1 AT2G22990 29.930 431 275 11 42 464 22 433 4.15e-57 195
MS.gene032088.t1 AT2G23000 29.748 437 270 12 42 464 24 437 9.10e-54 187
MS.gene032088.t1 AT2G23000 29.748 437 270 12 42 464 24 437 9.10e-54 187
MS.gene032088.t1 AT2G22920 29.425 435 280 10 38 464 20 435 2.59e-52 182
MS.gene032088.t1 AT2G22990 30.049 406 258 10 42 439 22 409 3.71e-52 183
MS.gene032088.t1 AT2G22990 30.123 405 257 10 42 438 22 408 5.30e-52 181
MS.gene032088.t1 AT3G10450 29.398 432 274 11 42 464 28 437 1.26e-51 181
MS.gene032088.t1 AT2G22970 29.792 433 271 13 42 464 24 433 4.39e-51 179
MS.gene032088.t1 AT2G23010 29.932 441 264 13 42 464 24 437 4.89e-51 179
MS.gene032088.t1 AT1G73280 29.398 432 274 11 42 464 32 441 5.67e-51 179
MS.gene032088.t1 AT2G22980 30.275 436 261 15 42 464 25 430 1.30e-50 178
MS.gene032088.t1 AT2G23010 29.705 441 265 13 42 464 24 437 2.17e-50 177
MS.gene032088.t1 AT3G12240 29.909 438 272 12 38 464 23 436 6.37e-50 176
MS.gene032088.t1 AT2G22990 28.538 431 272 12 42 464 22 424 2.47e-49 174
MS.gene032088.t1 AT1G73270 30.201 447 251 16 42 464 32 441 1.42e-48 173
MS.gene032088.t1 AT1G73270 30.425 447 250 17 42 464 32 441 1.67e-48 173
MS.gene032088.t1 AT3G12230 29.291 437 276 12 38 464 22 435 2.65e-48 172
MS.gene032088.t1 AT3G12220 30.455 440 261 14 38 461 22 432 3.13e-48 172
MS.gene032088.t1 AT1G33540 29.268 451 277 14 42 464 28 464 1.36e-47 171
MS.gene032088.t1 AT3G12203 30.233 430 264 13 46 464 12 416 1.43e-47 169
MS.gene032088.t1 AT1G73300 28.899 436 271 12 42 464 32 441 1.84e-47 170
MS.gene032088.t1 AT1G73300 28.899 436 271 12 42 464 32 441 1.84e-47 170
MS.gene032088.t1 AT3G10410 29.187 418 263 12 54 459 96 492 1.88e-47 171
MS.gene032088.t1 AT1G73290 29.120 443 275 13 35 464 22 438 1.92e-47 170
MS.gene032088.t1 AT3G12203 30.233 430 264 13 46 464 33 437 1.98e-47 170
MS.gene032088.t1 AT3G10450 28.814 413 263 11 61 464 4 394 2.04e-47 169
MS.gene032088.t1 AT2G22970 29.907 428 267 13 42 459 24 428 2.06e-47 170
MS.gene032088.t1 AT5G22980 28.842 423 260 14 55 461 96 493 8.26e-47 169
MS.gene032088.t1 AT2G22970 30.147 408 253 12 42 439 24 409 1.00e-46 167
MS.gene032088.t1 AT1G73310 29.099 433 274 12 42 464 32 441 1.45e-46 167
MS.gene032088.t1 AT5G36180 29.128 436 270 12 42 464 32 441 2.00e-46 167
MS.gene032088.t1 AT2G22980 30.414 411 244 14 42 439 25 406 3.48e-46 166
MS.gene032088.t1 AT3G10450 29.484 407 257 10 42 439 28 413 3.95e-46 166
MS.gene032088.t1 AT2G22980 29.691 421 254 14 42 449 25 416 5.55e-46 166
MS.gene032088.t1 AT5G09640 27.902 448 290 13 42 464 26 465 6.72e-46 166
MS.gene032088.t1 AT2G22920 29.949 394 251 8 38 424 20 395 2.13e-45 163
MS.gene032088.t1 AT1G33540 29.398 432 263 14 61 464 4 421 3.91e-45 163
MS.gene032088.t1 AT3G12220 30.456 417 245 14 61 461 15 402 1.03e-44 162
MS.gene032088.t1 AT3G12203 29.950 404 247 13 72 464 1 379 9.15e-44 158
MS.gene032088.t1 AT3G45010 27.314 443 269 16 54 475 98 508 1.09e-43 161
MS.gene032088.t1 AT3G10450 28.866 388 246 10 61 439 4 370 7.37e-42 153
MS.gene032088.t1 AT1G73310 29.353 402 253 11 42 434 32 411 2.57e-41 153
MS.gene032088.t1 AT1G73290 29.104 402 248 11 35 424 22 398 2.83e-41 152
MS.gene032088.t1 AT1G73280 34.884 301 159 11 42 311 32 326 2.45e-39 146
MS.gene032088.t1 AT2G22920 35.338 266 162 6 38 296 20 282 2.20e-38 142
MS.gene032088.t1 AT1G73290 34.354 294 166 10 35 309 22 307 2.38e-38 142
MS.gene032088.t1 AT1G15000 28.029 421 257 12 59 464 37 426 3.24e-38 145
MS.gene032088.t1 AT2G22970 35.985 264 154 8 42 296 24 281 3.94e-38 142
MS.gene032088.t1 AT2G22990 27.679 336 223 8 132 464 1 319 4.20e-37 139
MS.gene032088.t1 AT2G22980 37.175 269 142 11 42 296 25 280 7.00e-37 139
MS.gene032088.t1 AT2G22990 27.381 336 224 8 132 464 1 319 2.45e-36 137
MS.gene032088.t1 AT3G12220 35.039 254 151 7 38 284 22 268 2.02e-35 135
MS.gene032088.t1 AT1G73280 26.901 342 226 8 127 464 18 339 1.51e-33 129
MS.gene032088.t1 AT1G73280 26.647 334 221 8 135 464 1 314 1.16e-30 121
MS.gene032088.t1 AT5G36180 26.100 341 230 7 127 464 18 339 2.09e-29 118
MS.gene032088.t1 AT5G36180 26.100 341 230 7 127 464 18 339 2.09e-29 118
MS.gene032088.t1 AT2G22980 27.848 316 193 11 132 439 1 289 3.98e-27 110
MS.gene032088.t1 AT5G36180 27.329 322 201 9 127 439 18 315 2.05e-25 106
MS.gene032088.t1 AT1G73310 26.603 312 205 7 127 434 18 309 5.60e-25 105
MS.gene032088.t1 AT5G36180 26.254 339 208 9 127 446 18 333 1.94e-23 101
MS.gene032088.t1 AT5G22960 31.707 164 89 4 57 220 34 174 1.96e-23 97.8
MS.gene032088.t1 AT2G22920 23.333 270 188 5 198 464 3 256 9.31e-17 80.5
MS.gene032088.t1 AT2G27920 27.586 174 110 7 60 227 37 200 2.85e-15 78.2

Find 112 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGAGATTTATACGATTT+TGG 0.144394 2.3:+16330712 None:intergenic
AAAAGTCTGTTTCTTTATAT+TGG 0.157227 2.3:+16330913 None:intergenic
AAAGTCTGTTTCTTTATATT+GGG 0.209622 2.3:+16330914 None:intergenic
TTCCTACCGGACTGCGAATA+TGG 0.240472 2.3:-16329529 MS.gene032088:CDS
GGTTGTTCATCTATTGCTTA+TGG 0.243295 2.3:-16331625 MS.gene032088:CDS
GATGATTTCCATGATCAAAT+TGG 0.245394 2.3:-16330488 MS.gene032088:CDS
AAGAATAAGGATTAAGATAA+AGG 0.248695 2.3:+16331554 None:intergenic
TTTATCTTAATCCTTATTCT+TGG 0.248841 2.3:-16331552 MS.gene032088:CDS
TCACCTTGATTCCAAGAATA+AGG 0.275529 2.3:+16331541 None:intergenic
GACAATAACTTGGCTAAGTT+GGG 0.289583 2.3:+16330755 None:intergenic
TCTTGCTCTTGTTGGATAGA+TGG 0.290366 2.3:+16331820 None:intergenic
CAATCCTGTCTTGCTCTTGT+TGG 0.291839 2.3:+16331812 None:intergenic
ACGCGAGTCCGGCGTATTCT+TGG 0.309427 2.3:+16329236 None:intergenic
CGGAAGCTTGAGAGCATCTA+TGG 0.323326 2.3:+16329312 None:intergenic
TACCATTTGTCCACATAAAC+TGG 0.327933 2.3:+16330459 None:intergenic
ATCAACTTTGAACACTATTC+TGG 0.339761 2.3:-16331760 MS.gene032088:CDS
AACAATGCTAGCGCAAGTTT+TGG 0.341609 2.3:+16329169 None:intergenic
CTGATCATAGGCCTGCTAAA+TGG 0.369150 2.3:-16329993 MS.gene032088:CDS
GTAATGTGGTAGCTATTAAC+TGG 0.379476 2.3:-16329595 MS.gene032088:CDS
GACTGCGAATATGGATGTTT+AGG 0.384302 2.3:-16329520 MS.gene032088:intron
AATCCTTATTCTTGGAATCA+AGG 0.386899 2.3:-16331544 MS.gene032088:intron
CCATCTGAATTAATATGAAA+TGG 0.387970 2.3:+16331583 None:intergenic
TATGGTGAGGCTGAGGAAAT+TGG 0.388170 2.3:-16331607 MS.gene032088:CDS
CGCGAGTCCGGCGTATTCTT+GGG 0.395762 2.3:+16329237 None:intergenic
TGACAATAACTTGGCTAAGT+TGG 0.403269 2.3:+16330754 None:intergenic
GACTCGCGTTTGTAAATGTA+AGG 0.403885 2.3:-16329222 MS.gene032088:CDS
TATGATGATGGGGAGGTAGC+AGG 0.407095 2.3:-16329265 MS.gene032088:CDS
TTATGGTTCAATGGTGGTCC+TGG 0.414908 2.3:-16331646 MS.gene032088:CDS
ATATGATGTTGCATAGGAAA+AGG 0.424456 2.3:+16331860 None:intergenic
GATGAACATGTAGAATCCTT+TGG 0.426934 2.3:+16330016 None:intergenic
ATTGATTCTCCTGTTGGAGT+TGG 0.431935 2.3:-16331422 MS.gene032088:CDS
TTTGTAAATGTAAGGGGTGC+AGG 0.432886 2.3:-16329214 MS.gene032088:CDS
TGTAGAATCCTTTGGACCTC+TGG 0.432976 2.3:+16330024 None:intergenic
GGAACATTCCAATTTGATCA+TGG 0.434087 2.3:+16330480 None:intergenic
CTGCATGTCTGCCATTTAGC+AGG 0.435157 2.3:+16329982 None:intergenic
TTGTTCAAATCCTTCTTAGC+AGG 0.435419 2.3:-16329151 MS.gene032088:CDS
AGAATAAGGATTAAGATAAA+GGG 0.438471 2.3:+16331555 None:intergenic
TTGGAATGTTCCAGTTTATG+TGG 0.440613 2.3:-16330469 MS.gene032088:CDS
TACATTTACAAACGCGAGTC+CGG 0.442623 2.3:+16329225 None:intergenic
GAATAGCGAGTAGATGTTAC+CGG 0.444855 2.3:+16329334 None:intergenic
TTCCAGTTTATGTGGACAAA+TGG 0.445155 2.3:-16330461 MS.gene032088:CDS
TATGAAAATCCAACTCCAAC+AGG 0.449223 2.3:+16331413 None:intergenic
AGCTTCCGACTGTGAGCCCC+TGG 0.450261 2.3:-16329297 MS.gene032088:CDS
ATTAGTGGTGAGAGCTATGC+AGG 0.457911 2.3:-16330888 MS.gene032088:intron
ATCATCATACCATGCACGCC+AGG 0.458157 2.3:+16329279 None:intergenic
CTCTCATATTTCTATTGAAG+TGG 0.464353 2.3:-16330950 MS.gene032088:CDS
CCATTTCATATTAATTCAGA+TGG 0.467326 2.3:-16331583 MS.gene032088:CDS
ATGATGATGGGGAGGTAGCA+GGG 0.469653 2.3:-16329264 MS.gene032088:CDS
TACATGTTCATCCTGATCAT+AGG 0.476071 2.3:-16330005 MS.gene032088:CDS
CATCGAGGTTCCTGCTAAGA+AGG 0.478934 2.3:+16329141 None:intergenic
CTATCCAACAAGAGCAAGAC+AGG 0.480982 2.3:-16331816 MS.gene032088:CDS
CTGTGAGCCCCTGGCGTGCA+TGG 0.487146 2.3:-16329288 MS.gene032088:CDS
CTGCTAAATGGCAGACATGC+AGG 0.488766 2.3:-16329981 MS.gene032088:intron
TATGATGTTGCATAGGAAAA+GGG 0.505096 2.3:+16331861 None:intergenic
AAGTCTGTTTCTTTATATTG+GGG 0.506615 2.3:+16330915 None:intergenic
AGTTGTACTTGACAATAACT+TGG 0.508533 2.3:+16330745 None:intergenic
CATTTAGCAGGCCTATGATC+AGG 0.509453 2.3:+16329994 None:intergenic
TTCTCAACAATGGAGATAAG+AGG 0.509745 2.3:-16331370 MS.gene032088:CDS
GGAGTGGTAAAGATACTGTA+TGG 0.518484 2.3:+16330314 None:intergenic
GTCCGGTAGGAATAAGTTCT+CGG 0.527104 2.3:+16329540 None:intergenic
TGGCGTGCATGGTATGATGA+TGG 0.529388 2.3:-16329277 MS.gene032088:CDS
GTATGTAGTGCTGTGCTTCG+CGG 0.530361 2.3:+16330056 None:intergenic
GGCGTGCATGGTATGATGAT+GGG 0.531882 2.3:-16329276 MS.gene032088:CDS
CATATCAAGAGGCGGTTCTG+AGG 0.532956 2.3:+16329098 None:intergenic
TTCATCTATTGCTTATGGTG+AGG 0.535890 2.3:-16331620 MS.gene032088:CDS
ATTACAGTTAACAAGGATGT+TGG 0.539539 2.3:-16331733 MS.gene032088:CDS
GTTCAAAGATGTCATAACAT+TGG 0.542288 2.3:-16331887 MS.gene032088:CDS
GTGCTGTGCTTCGCGGTGCA+AGG 0.546901 2.3:+16330063 None:intergenic
AACAATGGAGATAAGAGGAC+AGG 0.550490 2.3:-16331365 MS.gene032088:intron
TACCGAGAACTTATTCCTAC+CGG 0.552693 2.3:-16329542 MS.gene032088:CDS
TCATCATACCATGCACGCCA+GGG 0.554763 2.3:+16329280 None:intergenic
TATAAATCTCAAAGGTTACA+TGG 0.563911 2.3:-16330703 MS.gene032088:intron
AGGCTAATATGATGTTGCAT+AGG 0.572347 2.3:+16331854 None:intergenic
CTAAAATGGTGAATTCTAAG+TGG 0.575651 2.3:-16331921 None:intergenic
AAACATCCATATTCGCAGTC+CGG 0.576303 2.3:+16329523 None:intergenic
TAATACAGATGCTGTACTCC+CGG 0.577320 2.3:-16329353 MS.gene032088:CDS
ATATCAAGAGGCGGTTCTGA+GGG 0.578391 2.3:+16329099 None:intergenic
GCACGCCAGGGGCTCACAGT+CGG 0.581620 2.3:+16329292 None:intergenic
CAAGAGGCGGTTCTGAGGGT+AGG 0.581980 2.3:+16329103 None:intergenic
CAAAATCGTATAAATCTCAA+AGG 0.583755 2.3:-16330711 MS.gene032088:CDS
TGGTTATATTACAGTTAACA+AGG 0.583867 2.3:-16331740 MS.gene032088:CDS
CCATTGAACCATAAAAGAAG+AGG 0.584648 2.3:+16331655 None:intergenic
CAGTTAACAAGGATGTTGGT+AGG 0.585358 2.3:-16331729 MS.gene032088:CDS
TCTTGTTCATTGCAGTAATG+TGG 0.586614 2.3:-16329609 MS.gene032088:intron
TCAATCTACCAGAGGTCCAA+AGG 0.587297 2.3:-16330032 MS.gene032088:CDS
ACAATAACTTGGCTAAGTTG+GGG 0.588566 2.3:+16330756 None:intergenic
TTGTAAATGTAAGGGGTGCA+GGG 0.591238 2.3:-16329213 MS.gene032088:CDS
TATTGCTTATGGTGAGGCTG+AGG 0.592286 2.3:-16331614 MS.gene032088:CDS
GCTTCTGATATTCTCAACAA+TGG 0.594486 2.3:-16331380 MS.gene032088:CDS
AGGGTAGGCAGTTCAAGAGT+CGG 0.594964 2.3:+16329118 None:intergenic
CAAGCTGATTATCATTAGCA+TGG 0.595628 2.3:+16330288 None:intergenic
TTTGAATGAATCATATCAAG+AGG 0.601292 2.3:+16329087 None:intergenic
TGTGGTAGCTATTAACTGGA+AGG 0.602523 2.3:-16329591 MS.gene032088:CDS
GCGTGCATGGTATGATGATG+GGG 0.605023 2.3:-16329275 MS.gene032088:CDS
CCGACTGAAAATCACATAGC+AGG 0.606165 2.3:+16330405 None:intergenic
ACTCGCGTTTGTAAATGTAA+GGG 0.610329 2.3:-16329221 MS.gene032088:CDS
CTCGCGTTTGTAAATGTAAG+GGG 0.612539 2.3:-16329220 MS.gene032088:CDS
TGATTATCATTAGCATGGCA+AGG 0.614829 2.3:+16330293 None:intergenic
ATCATTAGCATGGCAAGGAG+TGG 0.621689 2.3:+16330298 None:intergenic
GATAGATGGATCAGTTGCAA+AGG 0.626347 2.3:+16331834 None:intergenic
AGGATTGTAACAGCACTCCC+AGG 0.631686 2.3:-16331796 MS.gene032088:CDS
ATCCATATTCGCAGTCCGGT+AGG 0.635959 2.3:+16329527 None:intergenic
TTTGTCTTGTAACAGCGTGT+TGG 0.638516 2.3:-16330108 MS.gene032088:intron
AATAGCGAGTAGATGTTACC+GGG 0.643039 2.3:+16329335 None:intergenic
GAATGAATCATATCAAGAGG+CGG 0.652220 2.3:+16329090 None:intergenic
TGCATGGTATGATGATGGGG+AGG 0.665311 2.3:-16329272 MS.gene032088:CDS
TACATACTTCAATCTACCAG+AGG 0.676756 2.3:-16330040 MS.gene032088:CDS
GGGTGGACCCAAGAATACGC+CGG 0.680880 2.3:-16329244 MS.gene032088:CDS
TAAGCAATAGATGAACAACC+AGG 0.697743 2.3:+16331628 None:intergenic
ATGATGGGGAGGTAGCAGGG+TGG 0.707830 2.3:-16329261 MS.gene032088:CDS
CATCATACCATGCACGCCAG+GGG 0.710600 2.3:+16329281 None:intergenic
CAGTTCAAGAGTCGGCATCG+AGG 0.741560 2.3:+16329126 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATGTATATATTAGAAAAA+GGG + chr2.3:16331529-16331548 None:intergenic 10.0%
!! ACTATAAAATTATACAATTC+AGG + chr2.3:16330870-16330889 None:intergenic 15.0%
!! CATATGTATATATTAGAAAA+AGG + chr2.3:16331530-16331549 None:intergenic 15.0%
!!! ATATTTTAATGTAAGTAAGA+TGG + chr2.3:16331131-16331150 None:intergenic 15.0%
!!! CATAGAATACATTTTTTAAA+TGG + chr2.3:16329517-16329536 None:intergenic 15.0%
!!! TAACTAAATCATTTGATAAT+TGG - chr2.3:16331292-16331311 MS.gene032088:intron 15.0%
!! AAGAATAAGGATTAAGATAA+AGG + chr2.3:16329467-16329486 None:intergenic 20.0%
!! AAGTGACAAAAAAATCAATT+TGG - chr2.3:16331154-16331173 MS.gene032088:intron 20.0%
!! AGAATAAGGATTAAGATAAA+GGG + chr2.3:16329466-16329485 None:intergenic 20.0%
!! AGAATCAATAAAAAGAATGT+TGG + chr2.3:16329585-16329604 None:intergenic 20.0%
!! AGGTAAAAAATACATACAAA+TGG - chr2.3:16330150-16330169 MS.gene032088:intron 20.0%
!! TTTATCTTAATCCTTATTCT+TGG - chr2.3:16329466-16329485 MS.gene032088:intron 20.0%
!!! AAAAGTCTGTTTCTTTATAT+TGG + chr2.3:16330108-16330127 None:intergenic 20.0%
!!! AAAGTCTGTTTCTTTATATT+GGG + chr2.3:16330107-16330126 None:intergenic 20.0%
!!! ATAATTTGATTTGATTCCAT+TGG + chr2.3:16330018-16330037 None:intergenic 20.0%
!!! TATATTACCAAGTTCTTTTT+CGG + chr2.3:16330681-16330700 None:intergenic 20.0%
!!! TTGTGTGCTTTATTTTTTTT+AGG - chr2.3:16330799-16330818 MS.gene032088:intron 20.0%
! AAATGTTGAGAATAAATAGC+AGG - chr2.3:16330399-16330418 MS.gene032088:CDS 25.0%
! AACATTTATGCAATATTGCA+TGG + chr2.3:16330386-16330405 None:intergenic 25.0%
! ACATTTATGCAATATTGCAT+GGG + chr2.3:16330385-16330404 None:intergenic 25.0%
! CAAAATCGTATAAATCTCAA+AGG - chr2.3:16330307-16330326 MS.gene032088:CDS 25.0%
! CCATCTGAATTAATATGAAA+TGG + chr2.3:16329438-16329457 None:intergenic 25.0%
! CCATTTCATATTAATTCAGA+TGG - chr2.3:16329435-16329454 MS.gene032088:intron 25.0%
! CTTGTGAATTTATTATAACG+TGG + chr2.3:16331373-16331392 None:intergenic 25.0%
! GTAATGAGCATAAATTAAGA+GGG + chr2.3:16329735-16329754 None:intergenic 25.0%
! TATAAATCTCAAAGGTTACA+TGG - chr2.3:16330315-16330334 MS.gene032088:CDS 25.0%
! TCAGGATAAGAAAATGTTAT+AGG + chr2.3:16331586-16331605 None:intergenic 25.0%
! TGGTTATATTACAGTTAACA+AGG - chr2.3:16329278-16329297 MS.gene032088:CDS 25.0%
! TGTAATGAGCATAAATTAAG+AGG + chr2.3:16329736-16329755 None:intergenic 25.0%
! TTCCATAGTAGAAACTATAT+AGG - chr2.3:16331196-16331215 MS.gene032088:intron 25.0%
!! AAGTCTGTTTCTTTATATTG+GGG + chr2.3:16330106-16330125 None:intergenic 25.0%
!! TCTTATCCTGAATCAATTTT+GGG - chr2.3:16331595-16331614 MS.gene032088:CDS 25.0%
!! TTCTTATCCTGAATCAATTT+TGG - chr2.3:16331594-16331613 MS.gene032088:CDS 25.0%
!!! CTTTGAGATTTATACGATTT+TGG + chr2.3:16330309-16330328 None:intergenic 25.0%
!!! TATATATTGAGCACTGTTTT+AGG + chr2.3:16331458-16331477 None:intergenic 25.0%
AACATAATGTCCTGAAACAA+GGG + chr2.3:16330242-16330261 None:intergenic 30.0%
AATCCTTATTCTTGGAATCA+AGG - chr2.3:16329474-16329493 MS.gene032088:intron 30.0%
ACGTGGAAATAATTATACAG+TGG + chr2.3:16331356-16331375 None:intergenic 30.0%
AGAAAATGTTATAGGACTCA+TGG + chr2.3:16331578-16331597 None:intergenic 30.0%
AGAGTCATGTGAAAAAATCT+GGG - chr2.3:16330649-16330668 MS.gene032088:intron 30.0%
AGTTGTACTTGACAATAACT+TGG + chr2.3:16330276-16330295 None:intergenic 30.0%
ATAAGAGGACAGGTAAATAT+AGG - chr2.3:16329663-16329682 MS.gene032088:intron 30.0%
ATATGATGTTGCATAGGAAA+AGG + chr2.3:16329161-16329180 None:intergenic 30.0%
ATCAACTTTGAACACTATTC+TGG - chr2.3:16329258-16329277 MS.gene032088:CDS 30.0%
ATTACAGTTAACAAGGATGT+TGG - chr2.3:16329285-16329304 MS.gene032088:CDS 30.0%
GATGAATAAGCACATATGTA+AGG + chr2.3:16331553-16331572 None:intergenic 30.0%
GATGATTTCCATGATCAAAT+TGG - chr2.3:16330530-16330549 MS.gene032088:intron 30.0%
GTCCTATATAGTTTCTACTA+TGG + chr2.3:16331201-16331220 None:intergenic 30.0%
GTTCAAAGATGTCATAACAT+TGG - chr2.3:16329131-16329150 MS.gene032088:CDS 30.0%
TACAAATGGTGAGATAGATA+AGG - chr2.3:16330164-16330183 MS.gene032088:intron 30.0%
TATGATGTTGCATAGGAAAA+GGG + chr2.3:16329160-16329179 None:intergenic 30.0%
TTACATGCATTAACTGAACT+CGG - chr2.3:16329689-16329708 MS.gene032088:intron 30.0%
TTCTATGAGCTATGATGTAT+TGG - chr2.3:16329530-16329549 MS.gene032088:CDS 30.0%
TTTCAGGGGATAAAAGTTAA+GGG + chr2.3:16330843-16330862 None:intergenic 30.0%
! AGAATAGTATTTTGCTTCTC+TGG + chr2.3:16331241-16331260 None:intergenic 30.0%
! ATTTGGTTTCAATTTGGTCA+AGG + chr2.3:16329898-16329917 None:intergenic 30.0%
! CTCTAATGAACTAGTCATTT+TGG - chr2.3:16329939-16329958 MS.gene032088:intron 30.0%
! CTCTCATATTTCTATTGAAG+TGG - chr2.3:16330068-16330087 MS.gene032088:CDS 30.0%
! CTTCAAAACCTCTTCTTTTA+TGG - chr2.3:16329355-16329374 MS.gene032088:CDS 30.0%
! GCGTATATTTGGTTTCAATT+TGG + chr2.3:16329904-16329923 None:intergenic 30.0%
!! ATACAATTCAGGACTTTTTC+AGG + chr2.3:16330859-16330878 None:intergenic 30.0%
!! TTTTCAGGGGATAAAAGTTA+AGG + chr2.3:16330844-16330863 None:intergenic 30.0%
!!! AAAAGAAGAGGTTTTGAAGT+AGG + chr2.3:16329354-16329373 None:intergenic 30.0%
!!! ACATTGTTGCTTTTATTGAG+AGG - chr2.3:16329753-16329772 MS.gene032088:intron 30.0%
!!! ATTTTTTCACATGACTCTGA+CGG + chr2.3:16330647-16330666 None:intergenic 30.0%
!!! CTTTTTATTGATTCTCCTGT+TGG - chr2.3:16329590-16329609 MS.gene032088:CDS 30.0%
!!! GAAACAGACTTTTTCATTAG+TGG - chr2.3:16330115-16330134 MS.gene032088:intron 30.0%
!!! TACAATTCAGGACTTTTTCA+GGG + chr2.3:16330858-16330877 None:intergenic 30.0%
AACCAAATATACGCGTTTCT+AGG - chr2.3:16329910-16329929 MS.gene032088:intron 35.0%
ACAATAACTTGGCTAAGTTG+GGG + chr2.3:16330265-16330284 None:intergenic 35.0%
ACCAAATATACGCGTTTCTA+GGG - chr2.3:16329911-16329930 MS.gene032088:intron 35.0%
ACTCGCGTTTGTAAATGTAA+GGG - chr2.3:16331797-16331816 MS.gene032088:CDS 35.0%
AGGCTAATATGATGTTGCAT+AGG + chr2.3:16329167-16329186 None:intergenic 35.0%
ATAATCTTTATGTGCAGCTG+AGG - chr2.3:16330042-16330061 MS.gene032088:CDS 35.0%
CAAGACAAAGCATATGAATG+AGG + chr2.3:16330899-16330918 None:intergenic 35.0%
CAAGCTGATTATCATTAGCA+TGG + chr2.3:16330733-16330752 None:intergenic 35.0%
CAGAGTCATGTGAAAAAATC+TGG - chr2.3:16330648-16330667 MS.gene032088:intron 35.0%
CATACTGTGAAAGTAAGTAG+AGG + chr2.3:16331091-16331110 None:intergenic 35.0%
CCATTGAACCATAAAAGAAG+AGG + chr2.3:16329366-16329385 None:intergenic 35.0%
CCATTGGCATACATGAAAAT+TGG + chr2.3:16330002-16330021 None:intergenic 35.0%
CTGCAATGAACAAGAATAAG+AGG + chr2.3:16331404-16331423 None:intergenic 35.0%
GAAATTGCCGAAAAAGAACT+TGG - chr2.3:16330671-16330690 MS.gene032088:intron 35.0%
GAACATAATGTCCTGAAACA+AGG + chr2.3:16330243-16330262 None:intergenic 35.0%
GACAATAACTTGGCTAAGTT+GGG + chr2.3:16330266-16330285 None:intergenic 35.0%
GATGAACATGTAGAATCCTT+TGG + chr2.3:16331005-16331024 None:intergenic 35.0%
GCTTCTGATATTCTCAACAA+TGG - chr2.3:16329638-16329657 MS.gene032088:intron 35.0%
GGAACATTCCAATTTGATCA+TGG + chr2.3:16330541-16330560 None:intergenic 35.0%
GGTTGTTCATCTATTGCTTA+TGG - chr2.3:16329393-16329412 MS.gene032088:intron 35.0%
GTAATGTGGTAGCTATTAAC+TGG - chr2.3:16331423-16331442 MS.gene032088:CDS 35.0%
TAAGCAATAGATGAACAACC+AGG + chr2.3:16329393-16329412 None:intergenic 35.0%
TACATACTTCAATCTACCAG+AGG - chr2.3:16330978-16330997 MS.gene032088:intron 35.0%
TACATGTTCATCCTGATCAT+AGG - chr2.3:16331013-16331032 MS.gene032088:intron 35.0%
TACCATTTGTCCACATAAAC+TGG + chr2.3:16330562-16330581 None:intergenic 35.0%
TATGAAAATCCAACTCCAAC+AGG + chr2.3:16329608-16329627 None:intergenic 35.0%
TCACCTTGATTCCAAGAATA+AGG + chr2.3:16329480-16329499 None:intergenic 35.0%
TCTTGTTCATTGCAGTAATG+TGG - chr2.3:16331409-16331428 MS.gene032088:CDS 35.0%
TGACAATAACTTGGCTAAGT+TGG + chr2.3:16330267-16330286 None:intergenic 35.0%
TGATTATCATTAGCATGGCA+AGG + chr2.3:16330728-16330747 None:intergenic 35.0%
TGGCATACATGAAAATTGGT+TGG + chr2.3:16329998-16330017 None:intergenic 35.0%
TTCAGGGGATAAAAGTTAAG+GGG + chr2.3:16330842-16330861 None:intergenic 35.0%
TTCATCTATTGCTTATGGTG+AGG - chr2.3:16329398-16329417 MS.gene032088:intron 35.0%
TTCCAGTTTATGTGGACAAA+TGG - chr2.3:16330557-16330576 MS.gene032088:intron 35.0%
TTCTCAACAATGGAGATAAG+AGG - chr2.3:16329648-16329667 MS.gene032088:intron 35.0%
TTGGAATGTTCCAGTTTATG+TGG - chr2.3:16330549-16330568 MS.gene032088:intron 35.0%
TTGTTCAAATCCTTCTTAGC+AGG - chr2.3:16331867-16331886 MS.gene032088:CDS 35.0%
! CCAATTTTCATGTATGCCAA+TGG - chr2.3:16329999-16330018 MS.gene032088:CDS 35.0%
! CCTCTTCTTTTATGGTTCAA+TGG - chr2.3:16329363-16329382 MS.gene032088:intron 35.0%
! CTTCTTTTATGGTTCAATGG+TGG - chr2.3:16329366-16329385 MS.gene032088:intron 35.0%
! GTGTTTGAGTTCTTTGATTC+TGG - chr2.3:16330213-16330232 MS.gene032088:intron 35.0%
! TTGGTAGGACACTATTTTAC+TGG - chr2.3:16329304-16329323 MS.gene032088:CDS 35.0%
!! TGATAATTGGTAGTGACTGT+AGG - chr2.3:16331305-16331324 MS.gene032088:intron 35.0%
!! TGATGTTGAAGTTTTGACCT+GGG + chr2.3:16329242-16329261 None:intergenic 35.0%
!! TTGATGTTGAAGTTTTGACC+TGG + chr2.3:16329243-16329262 None:intergenic 35.0%
!!! ACAATTCAGGACTTTTTCAG+GGG + chr2.3:16330857-16330876 None:intergenic 35.0%
!!! TTTTTTCACATGACTCTGAC+GGG + chr2.3:16330646-16330665 None:intergenic 35.0%
AAACATCCATATTCGCAGTC+CGG + chr2.3:16331498-16331517 None:intergenic 40.0%
AAATTCCGCCACTAGTCATT+AGG - chr2.3:16330437-16330456 MS.gene032088:CDS 40.0%
AACAATGGAGATAAGAGGAC+AGG - chr2.3:16329653-16329672 MS.gene032088:intron 40.0%
AATAGCGAGTAGATGTTACC+GGG + chr2.3:16331686-16331705 None:intergenic 40.0%
ATTCTTGGAATCAAGGTGAG+TGG - chr2.3:16329481-16329500 MS.gene032088:intron 40.0%
CAGTTAACAAGGATGTTGGT+AGG - chr2.3:16329289-16329308 MS.gene032088:CDS 40.0%
CTCGCGTTTGTAAATGTAAG+GGG - chr2.3:16331798-16331817 MS.gene032088:CDS 40.0%
GAATAGCGAGTAGATGTTAC+CGG + chr2.3:16331687-16331706 None:intergenic 40.0%
GACTCGCGTTTGTAAATGTA+AGG - chr2.3:16331796-16331815 MS.gene032088:CDS 40.0%
GACTGCGAATATGGATGTTT+AGG - chr2.3:16331498-16331517 MS.gene032088:intron 40.0%
GATAGATGGATCAGTTGCAA+AGG + chr2.3:16329187-16329206 None:intergenic 40.0%
GGAAACAATGTAAGCTGCTT+TGG + chr2.3:16331623-16331642 None:intergenic 40.0%
GGAGTGGTAAAGATACTGTA+TGG + chr2.3:16330707-16330726 None:intergenic 40.0%
GTAAGACAGTAGTAGAAGCA+AGG + chr2.3:16330779-16330798 None:intergenic 40.0%
GTAGTGACTGTAGGAATTCT+AGG - chr2.3:16331314-16331333 MS.gene032088:intron 40.0%
TAATACAGATGCTGTACTCC+CGG - chr2.3:16331665-16331684 MS.gene032088:CDS 40.0%
TACATTTACAAACGCGAGTC+CGG + chr2.3:16331796-16331815 None:intergenic 40.0%
TACCGAGAACTTATTCCTAC+CGG - chr2.3:16331476-16331495 MS.gene032088:intron 40.0%
TATATTTCACTGTCTGCTGC+AGG - chr2.3:16330490-16330509 MS.gene032088:CDS 40.0%
TCCCTAGAAACGCGTATATT+TGG + chr2.3:16329915-16329934 None:intergenic 40.0%
TCTTGCTCTTGTTGGATAGA+TGG + chr2.3:16329201-16329220 None:intergenic 40.0%
TGTGGTAGCTATTAACTGGA+AGG - chr2.3:16331427-16331446 MS.gene032088:CDS 40.0%
TTTGTCTTGTAACAGCGTGT+TGG - chr2.3:16330910-16330929 MS.gene032088:CDS 40.0%
! AACAATGCTAGCGCAAGTTT+TGG + chr2.3:16331852-16331871 None:intergenic 40.0%
! ATTGATTCTCCTGTTGGAGT+TGG - chr2.3:16329596-16329615 MS.gene032088:CDS 40.0%
! CCTGCTATGTGATTTTCAGT+CGG - chr2.3:16330613-16330632 MS.gene032088:intron 40.0%
! TTGGCACCCAAAATTGATTC+AGG + chr2.3:16331604-16331623 None:intergenic 40.0%
! TTGTAAATGTAAGGGGTGCA+GGG - chr2.3:16331805-16331824 MS.gene032088:CDS 40.0%
! TTTGTAAATGTAAGGGGTGC+AGG - chr2.3:16331804-16331823 MS.gene032088:CDS 40.0%
AACTGAACTCGGCTATTGAG+AGG - chr2.3:16329700-16329719 MS.gene032088:intron 45.0%
AGACTTCGCCTAATGACTAG+TGG + chr2.3:16330448-16330467 None:intergenic 45.0%
ATCATTAGCATGGCAAGGAG+TGG + chr2.3:16330723-16330742 None:intergenic 45.0%
ATTAGTGGTGAGAGCTATGC+AGG - chr2.3:16330130-16330149 MS.gene032088:intron 45.0%
CAATCCTGTCTTGCTCTTGT+TGG + chr2.3:16329209-16329228 None:intergenic 45.0%
CACTGACACGACATGAATTG+AGG + chr2.3:16331644-16331663 None:intergenic 45.0%
CATTTAGCAGGCCTATGATC+AGG + chr2.3:16331027-16331046 None:intergenic 45.0%
CCGACTGAAAATCACATAGC+AGG + chr2.3:16330616-16330635 None:intergenic 45.0%
CTATCCAACAAGAGCAAGAC+AGG - chr2.3:16329202-16329221 MS.gene032088:CDS 45.0%
CTCAATTCATGTCGTGTCAG+TGG - chr2.3:16331642-16331661 MS.gene032088:CDS 45.0%
CTGATCATAGGCCTGCTAAA+TGG - chr2.3:16331025-16331044 MS.gene032088:intron 45.0%
GTCCGGTAGGAATAAGTTCT+CGG + chr2.3:16331481-16331500 None:intergenic 45.0%
TATGGTGAGGCTGAGGAAAT+TGG - chr2.3:16329411-16329430 MS.gene032088:intron 45.0%
TATTGCTTATGGTGAGGCTG+AGG - chr2.3:16329404-16329423 MS.gene032088:intron 45.0%
TCAATCTACCAGAGGTCCAA+AGG - chr2.3:16330986-16331005 MS.gene032088:intron 45.0%
TGTAGAATCCTTTGGACCTC+TGG + chr2.3:16330997-16331016 None:intergenic 45.0%
TTATGGTTCAATGGTGGTCC+TGG - chr2.3:16329372-16329391 MS.gene032088:intron 45.0%
!! ATTCTGGCTTCCCTTGTTTC+AGG - chr2.3:16330229-16330248 MS.gene032088:intron 45.0%
!! GCAAGTTTTGGTCTATGCAG+AGG + chr2.3:16331840-16331859 None:intergenic 45.0%
AGGATTGTAACAGCACTCCC+AGG - chr2.3:16329222-16329241 MS.gene032088:CDS 50.0%
AGGGTAGGCAGTTCAAGAGT+CGG + chr2.3:16331903-16331922 None:intergenic 50.0%
ATCATCATACCATGCACGCC+AGG + chr2.3:16331742-16331761 None:intergenic 50.0%
ATCCATATTCGCAGTCCGGT+AGG + chr2.3:16331494-16331513 None:intergenic 50.0%
CATCGAGGTTCCTGCTAAGA+AGG + chr2.3:16331880-16331899 None:intergenic 50.0%
CTGCTAAATGGCAGACATGC+AGG - chr2.3:16331037-16331056 MS.gene032088:intron 50.0%
CTTCGCCTAATGACTAGTGG+CGG + chr2.3:16330445-16330464 None:intergenic 50.0%
GGCGTGCATGGTATGATGAT+GGG - chr2.3:16331742-16331761 MS.gene032088:CDS 50.0%
GTATGTAGTGCTGTGCTTCG+CGG + chr2.3:16330965-16330984 None:intergenic 50.0%
TCATCATACCATGCACGCCA+GGG + chr2.3:16331741-16331760 None:intergenic 50.0%
TGGCGTGCATGGTATGATGA+TGG - chr2.3:16331741-16331760 MS.gene032088:CDS 50.0%
TTCCTACCGGACTGCGAATA+TGG - chr2.3:16331489-16331508 MS.gene032088:intron 50.0%
TTTCACTGTCTGCTGCAGGT+AGG - chr2.3:16330494-16330513 MS.gene032088:CDS 50.0%
! CTGCATGTCTGCCATTTAGC+AGG + chr2.3:16331039-16331058 None:intergenic 50.0%
! GAGAGGTTGACTTCCCGAAA+GGG - chr2.3:16329770-16329789 MS.gene032088:intron 50.0%
! GCGTGCATGGTATGATGATG+GGG - chr2.3:16331743-16331762 MS.gene032088:CDS 50.0%
! TGAGAGGTTGACTTCCCGAA+AGG - chr2.3:16329769-16329788 MS.gene032088:intron 50.0%
!! ATGATGATGGGGAGGTAGCA+GGG - chr2.3:16331754-16331773 MS.gene032088:CDS 50.0%
!! CGGAAGCTTGAGAGCATCTA+TGG + chr2.3:16331709-16331728 None:intergenic 50.0%
!! TATGATGATGGGGAGGTAGC+AGG - chr2.3:16331753-16331772 MS.gene032088:CDS 50.0%
!! TGCATGGTATGATGATGGGG+AGG - chr2.3:16331746-16331765 MS.gene032088:CDS 50.0%
CAGTTCAAGAGTCGGCATCG+AGG + chr2.3:16331895-16331914 None:intergenic 55.0%
CATCATACCATGCACGCCAG+GGG + chr2.3:16331740-16331759 None:intergenic 55.0%
CTCCGTGTGGACATCCCTTT+CGG + chr2.3:16329787-16329806 None:intergenic 55.0%
GAAAGGGATGTCCACACGGA+GGG - chr2.3:16329786-16329805 MS.gene032088:intron 55.0%
GTCGCATGACTCTCGAACTG+TGG - chr2.3:16329851-16329870 MS.gene032088:intron 55.0%
TCCCGAAAGGGATGTCCACA+CGG - chr2.3:16329782-16329801 MS.gene032088:intron 55.0%
TCCGTGTGGACATCCCTTTC+GGG + chr2.3:16329786-16329805 None:intergenic 55.0%
!! TCCACACGGAGGGTTTTGAC+AGG - chr2.3:16329796-16329815 MS.gene032088:intron 55.0%
ACGCGAGTCCGGCGTATTCT+TGG + chr2.3:16331785-16331804 None:intergenic 60.0%
CGAAAGGGATGTCCACACGG+AGG - chr2.3:16329785-16329804 MS.gene032088:intron 60.0%
CGCGAGTCCGGCGTATTCTT+GGG + chr2.3:16331784-16331803 None:intergenic 60.0%
GCCTGTCAAAACCCTCCGTG+TGG + chr2.3:16329800-16329819 None:intergenic 60.0%
GGGTGGACCCAAGAATACGC+CGG - chr2.3:16331774-16331793 MS.gene032088:CDS 60.0%
! ATGATGGGGAGGTAGCAGGG+TGG - chr2.3:16331757-16331776 MS.gene032088:CDS 60.0%
!! CAAGAGGCGGTTCTGAGGGT+AGG + chr2.3:16331918-16331937 None:intergenic 60.0%
AGCTTCCGACTGTGAGCCCC+TGG - chr2.3:16331721-16331740 MS.gene032088:CDS 65.0%
GTGCTGTGCTTCGCGGTGCA+AGG + chr2.3:16330958-16330977 None:intergenic 65.0%
CTGTGAGCCCCTGGCGTGCA+TGG - chr2.3:16331730-16331749 MS.gene032088:CDS 70.0%
GCACGCCAGGGGCTCACAGT+CGG + chr2.3:16331729-16331748 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 16329102 16331938 16329102 ID=MS.gene032088
chr2.3 mRNA 16329102 16331938 16329102 ID=MS.gene032088.t1;Parent=MS.gene032088
chr2.3 exon 16331545 16331938 16331545 ID=MS.gene032088.t1.exon1;Parent=MS.gene032088.t1
chr2.3 CDS 16331545 16331938 16331545 ID=cds.MS.gene032088.t1;Parent=MS.gene032088.t1
chr2.3 exon 16331366 16331461 16331366 ID=MS.gene032088.t1.exon2;Parent=MS.gene032088.t1
chr2.3 CDS 16331366 16331461 16331366 ID=cds.MS.gene032088.t1;Parent=MS.gene032088.t1
chr2.3 exon 16330889 16330981 16330889 ID=MS.gene032088.t1.exon3;Parent=MS.gene032088.t1
chr2.3 CDS 16330889 16330981 16330889 ID=cds.MS.gene032088.t1;Parent=MS.gene032088.t1
chr2.3 exon 16330704 16330789 16330704 ID=MS.gene032088.t1.exon4;Parent=MS.gene032088.t1
chr2.3 CDS 16330704 16330789 16330704 ID=cds.MS.gene032088.t1;Parent=MS.gene032088.t1
chr2.3 exon 16330274 16330528 16330274 ID=MS.gene032088.t1.exon5;Parent=MS.gene032088.t1
chr2.3 CDS 16330274 16330528 16330274 ID=cds.MS.gene032088.t1;Parent=MS.gene032088.t1
chr2.3 exon 16329982 16330115 16329982 ID=MS.gene032088.t1.exon6;Parent=MS.gene032088.t1
chr2.3 CDS 16329982 16330115 16329982 ID=cds.MS.gene032088.t1;Parent=MS.gene032088.t1
chr2.3 exon 16329521 16329616 16329521 ID=MS.gene032088.t1.exon7;Parent=MS.gene032088.t1
chr2.3 CDS 16329521 16329616 16329521 ID=cds.MS.gene032088.t1;Parent=MS.gene032088.t1
chr2.3 exon 16329102 16329378 16329102 ID=MS.gene032088.t1.exon8;Parent=MS.gene032088.t1
chr2.3 CDS 16329102 16329378 16329102 ID=cds.MS.gene032088.t1;Parent=MS.gene032088.t1
Gene Sequence

>MS.gene032088

ATGGTGAATTCTAAGTGGTCTCAAATTTTGTTCAAAGATGTCATAACATTGGCAGCCCTTTTCCTATGCAACATCATATTAGCCTTTGCAACTGATCCATCTATCCAACAAGAGCAAGACAGGATTGTAACAGCACTCCCAGGTCAAAACTTCAACATCAACTTTGAACACTATTCTGGTTATATTACAGTTAACAAGGATGTTGGTAGGACACTATTTTACTGGTTCATTGAAGCAGATCATATAGATCCTACTTCAAAACCTCTTCTTTTATGGTTCAATGGTGGTCCTGGTTGTTCATCTATTGCTTATGGTGAGGCTGAGGAAATTGGTCCATTTCATATTAATTCAGATGGCAATACCCTTTATCTTAATCCTTATTCTTGGAATCAAGGTGAGTGGTTGTGTTTGACCATTTAAAAAATGTATTCTATGAGCTATGATGTATTGGATCATTTCATTTTGATTTTTTTGCAGTTGCCAACATTCTTTTTATTGATTCTCCTGTTGGAGTTGGATTTTCATATTCAAATGATGCTTCTGATATTCTCAACAATGGAGATAAGAGGACAGGTAAATATAGGCATTTACATGCATTAACTGAACTCGGCTATTGAGAGGAAGAATTGTCCCTCTTAATTTATGCTCATTACATTGTTGCTTTTATTGAGAGGTTGACTTCCCGAAAGGGATGTCCACACGGAGGGTTTTGACAGGCGAGAAAGTTAAGTGTAGTGTCTCTGAGTATAGTCGCATGACTCTCGAACTGTGGCACATTGTAGAATTATTTTTGCCTTGACCAAATTGAAACCAAATATACGCGTTTCTAGGGATTTTCTCTAATGAACTAGTCATTTTGGATGAGTTCTTCTTTTGACACTCATAAAACTTAACCAACCAATTTTCATGTATGCCAATGGAATCAAATCAAATTATATTAATAATCTTTATGTGCAGCTGAGGACTCTCTCATATTTCTATTGAAGTGGTTCGAGCGTTTTCCCCAATATAAAGAAACAGACTTTTTCATTAGTGGTGAGAGCTATGCAGGTAAAAAATACATACAAATGGTGAGATAGATAAGGAAACTTTATCATGTCATCATTGTCATGTGTTTGAGTTCTTTGATTCTGGCTTCCCTTGTTTCAGGACATTATGTTCCCCAACTTAGCCAAGTTATTGTCAAGTACAACTCAGCTACAAACCAAAATCGTATAAATCTCAAAGGTTACATGGTATGAATTCTTTTTTTTTTTTTTTGCATAAAAACTAGTAAATATCCCATGCAATATTGCATAAATGTTGAGAATAAATAGCAGGAAAAAAAAGCATGTGAAATTCCGCCACTAGTCATTAGGCGAAGTCTTTTCTTAGAACTCAATCAATAGTATATTTCACTGTCTGCTGCAGGTAGGAAATGCTCTAACAGATGATTTCCATGATCAAATTGGAATGTTCCAGTTTATGTGGACAAATGGTATGATTTCAGATCAAACATTCAAGCTCTTGAACCTGCTATGTGATTTTCAGTCGGTCAAACACCCGTCAGAGTCATGTGAAAAAATCTGGGAAATTGCCGAAAAAGAACTTGGTAATATAGATCCATACAGTATCTTTACCACTCCTTGCCATGCTAATGATAATCAGCTTGTTATAAAAAAGCATGTGAGTCTACCTTGCTTCTACTACTGTCTTACTTGTGTGCTTTATTTTTTTTAGGCTTAAATGCATTTTTAACCCCTTAACTTTTATCCCCTGAAAAAGTCCTGAATTGTATAATTTTATAGTACAAATCCTCATTCATATGCTTTGTCTTGTAACAGCGTGTTGGAAATCTCAAAACTGTATATGATCCTTGCACCGCGAAGCACAGCACTACATACTTCAATCTACCAGAGGTCCAAAGGATTCTACATGTTCATCCTGATCATAGGCCTGCTAAATGGCAGACATGCAGGTAATTCTCATAAGAGAAACTTTTGAAAGCCTCTACTTACTTTCACAGTATGTGCTATAAATCGATTAACCATCTTACTTACATTAAAATATAGCAAGTGACAAAAAAATCAATTTGGAGCAAGCTTCTACGATGAGTTCCATAGTAGAAACTATATAGGACTGAAAACAGAAATACTACCAGAGAAGCAAAATACTATTCTGCACTATTAACTAACAAGTCAATGTTTCTTGTAACTAAATCATTTGATAATTGGTAGTGACTGTAGGAATTCTAGGCACAGCATGAAGTGCACCACTGTATAATTATTTCCACGTTATAATAAATTCACAAGATCAGAATCCTCTTATTCTTGTTCATTGCAGTAATGTGGTAGCTATTAACTGGAAGGATTCTCCTAAAACAGTGCTCAATATATACCGAGAACTTATTCCTACCGGACTGCGAATATGGATGTTTAGGTTATTCCCTTTTTCTAATATATACATATGCCTTACATATGTGCTTATTCATCTGCCATGAGTCCTATAACATTTTCTTATCCTGAATCAATTTTGGGTGCCAAAGCAGCTTACATTGTTTCCTCAATTCATGTCGTGTCAGTGGTAATACAGATGCTGTACTCCCGGTAACATCTACTCGCTATTCCATAGATGCTCTCAAGCTTCCGACTGTGAGCCCCTGGCGTGCATGGTATGATGATGGGGAGGTAGCAGGGTGGACCCAAGAATACGCCGGACTCGCGTTTGTAAATGTAAGGGGTGCAGGGCACGAAGTTCCTCTGCATAGACCAAAACTTGCGCTAGCATTGTTCAAATCCTTCTTAGCAGGAACCTCGATGCCGACTCTTGAACTGCCTACCCTCAGAACCGCCTCTTGA
Protein sequence

>MS.gene032088.t1

MVNSKWSQILFKDVITLAALFLCNIILAFATDPSIQQEQDRIVTALPGQNFNINFEHYSGYITVNKDVGRTLFYWFIEADHIDPTSKPLLLWFNGGPGCSSIAYGEAEEIGPFHINSDGNTLYLNPYSWNQVANILFIDSPVGVGFSYSNDASDILNNGDKRTAEDSLIFLLKWFERFPQYKETDFFISGESYAGHYVPQLSQVIVKYNSATNQNRINLKGYMVGNALTDDFHDQIGMFQFMWTNGMISDQTFKLLNLLCDFQSVKHPSESCEKIWEIAEKELGNIDPYSIFTTPCHANDNQLVIKKHRVGNLKTVYDPCTAKHSTTYFNLPEVQRILHVHPDHRPAKWQTCSNVVAINWKDSPKTVLNIYRELIPTGLRIWMFSGNTDAVLPVTSTRYSIDALKLPTVSPWRAWYDDGEVAGWTQEYAGLAFVNVRGAGHEVPLHRPKLALALFKSFLAGTSMPTLELPTLRTAS