AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003427

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003427.t1 MTR_4g075150 95.573 384 14 1 4 387 85 465 0.0 768
MS.gene003427.t1 MTR_4g075150 95.078 386 14 2 4 387 85 467 0.0 763
MS.gene003427.t1 MTR_7g013790 48.705 386 187 7 4 384 86 465 3.51e-130 382
MS.gene003427.t1 MTR_4g122950 48.724 392 185 7 4 383 90 477 3.59e-129 380
MS.gene003427.t1 MTR_4g089155 47.917 384 194 4 4 383 104 485 7.99e-128 377
MS.gene003427.t1 MTR_2g012860 49.091 385 188 5 4 383 92 473 8.00e-128 376
MS.gene003427.t1 MTR_3g034290 48.556 381 180 7 4 383 125 490 1.55e-126 374
MS.gene003427.t1 MTR_1g095560 48.953 382 182 7 4 383 86 456 4.42e-126 371
MS.gene003427.t1 MTR_2g012850 47.135 384 197 4 4 384 86 466 1.80e-125 370
MS.gene003427.t1 MTR_1g095540 48.819 381 184 6 4 383 86 456 4.88e-125 369
MS.gene003427.t1 MTR_3g079560 48.831 385 175 9 4 386 129 493 7.33e-125 369
MS.gene003427.t1 MTR_1g021010 49.344 381 173 6 4 384 127 487 3.30e-124 367
MS.gene003427.t1 MTR_0003s0560 47.244 381 183 6 4 383 124 487 5.41e-124 367
MS.gene003427.t1 MTR_4g122960 47.059 391 191 8 4 383 90 475 8.04e-124 366
MS.gene003427.t1 MTR_3g079620 47.704 392 177 8 4 386 129 501 1.38e-122 363
MS.gene003427.t1 MTR_3g079570 48.831 385 174 8 4 386 130 493 1.86e-122 363
MS.gene003427.t1 MTR_3g079590 48.930 374 173 6 4 377 129 484 3.97e-122 362
MS.gene003427.t1 MTR_2g062980 45.932 381 195 6 4 383 92 462 4.17e-120 356
MS.gene003427.t1 MTR_1g020090 47.244 381 178 6 4 384 141 498 7.15e-120 357
MS.gene003427.t1 MTR_1g095560 45.169 414 182 8 4 383 86 488 1.19e-119 356
MS.gene003427.t1 MTR_3g079600 48.138 376 176 7 4 377 127 485 4.75e-119 354
MS.gene003427.t1 MTR_2g062990 45.932 381 195 6 4 383 92 462 8.04e-118 350
MS.gene003427.t1 MTR_2g063000 45.692 383 195 7 4 383 91 463 2.30e-116 347
MS.gene003427.t1 MTR_1g021020 47.507 381 179 6 4 384 129 488 1.03e-114 343
MS.gene003427.t1 MTR_3g079630 46.753 385 173 10 4 377 129 492 8.66e-112 336
MS.gene003427.t1 MTR_2g081620 44.882 381 197 7 4 382 93 462 1.36e-110 332
MS.gene003427.t1 MTR_3g090130 41.753 388 208 9 4 383 79 456 6.66e-96 294
MS.gene003427.t1 MTR_3g090150 41.282 390 211 9 4 384 84 464 9.26e-95 291
MS.gene003427.t1 MTR_2g063000 40.420 381 187 7 4 383 91 432 1.36e-93 287
MS.gene003427.t1 MTR_5g072570 40.204 393 213 10 4 384 80 462 2.60e-93 288
MS.gene003427.t1 MTR_3g034290 47.475 297 141 6 6 302 127 408 1.12e-90 279
MS.gene003427.t1 MTR_2g449850 40.253 395 200 10 4 383 89 462 1.36e-90 280
MS.gene003427.t1 MTR_3g434850 39.594 394 204 12 4 383 81 454 1.36e-87 273
MS.gene003427.t1 MTR_7g021130 36.896 393 219 10 6 385 83 459 5.72e-84 263
MS.gene003427.t1 MTR_8g099930 37.817 394 218 10 4 384 84 463 1.22e-83 263
MS.gene003427.t1 MTR_1g040370 37.371 388 186 7 4 385 81 417 1.84e-80 253
MS.gene003427.t1 MTR_3g498755 36.020 397 222 10 4 384 66 446 1.09e-79 252
MS.gene003427.t1 MTR_7g051430 33.012 415 232 12 4 377 81 490 6.72e-68 223
MS.gene003427.t1 MTR_8g011350 34.048 420 230 14 4 382 82 495 1.84e-66 219
MS.gene003427.t1 MTR_7g080180 34.346 428 222 17 4 382 81 498 1.06e-65 217
MS.gene003427.t1 MTR_0003s0560 50.244 205 97 3 4 208 124 323 7.27e-64 208
MS.gene003427.t1 MTR_8g011350 33.588 393 214 14 4 355 82 468 1.32e-56 192
MS.gene003427.t1 MTR_7g080180 33.943 383 201 15 4 341 81 456 1.32e-54 187
MS.gene003427.t1 MTR_1g102130 32.061 393 210 12 6 385 144 492 3.96e-52 181
MS.gene003427.t1 MTR_2g018600 29.771 393 258 11 2 382 83 469 3.47e-49 172
MS.gene003427.t1 MTR_2g018590 30.633 395 253 13 2 382 82 469 1.58e-48 171
MS.gene003427.t1 MTR_7g051430 32.153 339 185 11 4 302 81 414 3.35e-46 163
MS.gene003427.t1 MTR_4g021500 30.127 395 253 12 2 384 90 473 1.38e-45 168
MS.gene003427.t1 MTR_4g021500 28.205 390 240 12 4 382 527 887 1.79e-39 150
MS.gene003427.t1 MTR_4g021500 30.051 396 254 12 2 385 90 474 2.02e-45 167
MS.gene003427.t1 MTR_4g021500 28.861 395 249 13 4 382 527 905 1.00e-40 154
MS.gene003427.t1 MTR_2g018630 29.337 392 254 11 2 382 86 465 9.26e-45 160
MS.gene003427.t1 MTR_4g021520 29.049 389 260 10 2 382 90 470 1.22e-44 160
MS.gene003427.t1 MTR_4g021535 28.792 389 257 11 4 382 88 466 2.54e-44 159
MS.gene003427.t1 MTR_4g021530 28.021 389 260 11 4 382 92 470 4.11e-43 156
MS.gene003427.t1 MTR_4g021490 27.692 390 260 11 4 382 92 470 2.80e-42 154
MS.gene003427.t1 MTR_2g018630 29.396 364 239 9 26 382 8 360 2.97e-42 152
MS.gene003427.t1 MTR_3g100420 29.367 395 260 10 2 382 107 496 7.29e-42 154
MS.gene003427.t1 MTR_4g021510 28.429 401 247 11 2 382 89 469 1.94e-40 149
MS.gene003427.t1 MTR_4g021520 29.107 347 231 9 2 341 90 428 9.13e-36 135
MS.gene003427.t1 MTR_3g045770 24.422 389 264 11 3 377 77 449 3.09e-34 132
MS.gene003427.t1 MTR_2g095750 28.101 395 238 14 2 380 134 498 2.06e-33 130
MS.gene003427.t1 MTR_2g095730 28.974 390 244 15 2 380 128 495 4.23e-33 129
MS.gene003427.t1 MTR_4g057570 27.500 400 222 15 2 373 124 483 2.56e-30 121
MS.gene003427.t1 MTR_1g094155 28.030 396 224 15 2 374 124 481 7.17e-30 120
MS.gene003427.t1 MTR_4g057585 26.750 400 229 14 2 377 131 490 5.18e-29 118
MS.gene003427.t1 MTR_5g097180 25.130 386 249 10 6 382 83 437 5.93e-29 117
MS.gene003427.t1 MTR_4g057595 26.750 400 229 14 2 377 132 491 7.01e-29 117
MS.gene003427.t1 MTR_4g021535 24.679 389 226 11 4 382 88 419 2.91e-27 112
MS.gene003427.t1 MTR_4g057600 26.531 392 246 13 2 380 130 492 7.59e-26 108
MS.gene003427.t1 MTR_2g009200 23.691 401 241 13 2 377 74 434 1.10e-25 108
MS.gene003427.t1 MTR_1g050545 27.089 395 229 16 2 374 118 475 4.46e-25 107
MS.gene003427.t1 MTR_4g084710 25.193 389 249 11 4 382 83 439 2.39e-22 98.6
MS.gene003427.t1 MTR_2g095710 26.368 402 228 18 2 380 125 481 6.65e-21 94.4
MS.gene003427.t1 MTR_5g021570 37.500 104 51 2 107 210 271 360 3.41e-14 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003427.t1 AT5G23210 67.246 403 110 3 3 387 5 403 0.0 584
MS.gene003427.t1 AT5G23210 67.750 400 111 2 4 387 102 499 0.0 583
MS.gene003427.t1 AT5G23210 67.750 400 107 3 6 387 9 404 0.0 583
MS.gene003427.t1 AT5G23210 67.139 353 98 2 3 339 5 355 0.0 509
MS.gene003427.t1 AT5G23210 67.330 352 97 2 4 339 102 451 6.74e-180 508
MS.gene003427.t1 AT5G08260 50.388 387 184 3 4 383 91 476 3.33e-145 421
MS.gene003427.t1 AT4G30610 49.741 386 183 7 4 384 84 463 2.78e-133 390
MS.gene003427.t1 AT3G02110 48.232 396 179 8 4 384 88 472 8.02e-130 381
MS.gene003427.t1 AT2G35780 49.738 382 177 7 4 383 83 451 1.16e-128 377
MS.gene003427.t1 AT1G61130 50.000 378 180 4 4 377 82 454 1.54e-128 377
MS.gene003427.t1 AT2G24010 48.187 386 181 5 4 384 81 452 8.90e-127 373
MS.gene003427.t1 AT2G24010 47.815 389 184 5 1 384 93 467 9.93e-127 373
MS.gene003427.t1 AT2G24000 49.610 385 186 5 4 384 82 462 5.67e-126 371
MS.gene003427.t1 AT3G63470 48.042 383 179 8 4 383 132 497 5.93e-126 372
MS.gene003427.t1 AT3G07990 48.294 381 186 7 4 383 87 457 2.22e-124 367
MS.gene003427.t1 AT3G17180 47.607 397 185 9 4 386 91 478 3.71e-124 367
MS.gene003427.t1 AT4G15100 48.438 384 185 5 4 377 95 475 1.48e-123 366
MS.gene003427.t1 AT1G11080 48.062 387 184 5 4 377 101 483 5.13e-122 362
MS.gene003427.t1 AT1G11080 48.062 387 184 5 4 377 101 483 7.88e-122 362
MS.gene003427.t1 AT2G24010 44.928 414 184 6 1 384 64 463 3.15e-121 359
MS.gene003427.t1 AT4G30810 46.719 381 195 5 6 385 91 464 9.67e-117 348
MS.gene003427.t1 AT2G35770 44.961 387 195 10 4 386 89 461 4.12e-109 328
MS.gene003427.t1 AT3G17180 46.974 347 162 8 6 339 93 430 2.90e-106 320
MS.gene003427.t1 AT3G52020 42.602 392 196 12 4 386 130 501 6.08e-99 303
MS.gene003427.t1 AT2G05850 41.071 392 203 11 4 386 115 487 6.31e-98 300
MS.gene003427.t1 AT3G52010 41.388 389 201 11 4 384 116 485 2.78e-96 296
MS.gene003427.t1 AT3G52000 41.818 385 201 10 4 384 115 480 4.26e-96 295
MS.gene003427.t1 AT1G43780 40.355 394 212 9 4 384 88 471 1.14e-94 291
MS.gene003427.t1 AT2G12480 39.487 390 218 9 4 386 82 460 9.61e-92 283
MS.gene003427.t1 AT5G42240 39.646 396 213 10 4 385 83 466 2.31e-90 280
MS.gene003427.t1 AT5G42230 39.796 392 214 10 4 383 79 460 8.82e-89 276
MS.gene003427.t1 AT5G42230 39.796 392 214 10 4 383 79 460 8.82e-89 276
MS.gene003427.t1 AT2G12480 38.718 390 197 10 4 386 82 436 1.19e-85 267
MS.gene003427.t1 AT2G24010 44.625 307 151 5 1 302 64 356 1.68e-85 264
MS.gene003427.t1 AT2G12480 37.949 390 198 10 4 386 82 434 5.76e-84 263
MS.gene003427.t1 AT2G33530 37.783 397 216 10 4 385 84 464 3.51e-83 261
MS.gene003427.t1 AT2G12480 37.852 391 198 11 4 386 82 435 3.20e-82 258
MS.gene003427.t1 AT1G28110 36.456 395 222 10 4 385 82 460 5.62e-81 256
MS.gene003427.t1 AT1G28110 36.456 395 222 10 4 385 82 460 5.62e-81 256
MS.gene003427.t1 AT2G24000 45.423 284 148 4 105 384 119 399 1.39e-78 248
MS.gene003427.t1 AT3G25420 35.084 419 227 12 4 382 81 494 3.24e-77 247
MS.gene003427.t1 AT4G12910 36.342 421 217 15 4 382 86 497 2.52e-73 237
MS.gene003427.t1 AT4G12910 35.620 379 194 14 4 341 86 455 4.47e-61 204
MS.gene003427.t1 AT2G22990 30.649 385 236 13 4 382 74 433 2.35e-45 162
MS.gene003427.t1 AT2G22980 32.143 392 215 15 4 382 77 430 8.36e-45 160
MS.gene003427.t1 AT1G73300 31.026 390 230 14 2 382 82 441 2.32e-44 159
MS.gene003427.t1 AT1G73300 31.026 390 230 14 2 382 82 441 2.32e-44 159
MS.gene003427.t1 AT1G73290 30.471 361 219 11 4 359 83 416 8.23e-43 155
MS.gene003427.t1 AT2G22920 29.820 389 238 13 2 382 74 435 1.06e-42 154
MS.gene003427.t1 AT3G10450 28.424 387 244 12 2 382 35 394 1.40e-42 153
MS.gene003427.t1 AT3G10450 28.424 387 244 12 2 382 78 437 3.61e-42 153
MS.gene003427.t1 AT2G22990 31.476 359 216 12 4 356 74 408 4.34e-42 152
MS.gene003427.t1 AT2G22990 31.111 360 218 12 4 357 74 409 4.67e-42 153
MS.gene003427.t1 AT1G73280 29.610 385 238 12 4 382 84 441 7.34e-42 152
MS.gene003427.t1 AT3G12203 29.752 363 218 11 26 382 48 379 9.10e-42 151
MS.gene003427.t1 AT1G73270 29.668 391 234 15 2 382 82 441 1.06e-41 152
MS.gene003427.t1 AT5G36180 30.513 390 232 14 2 382 82 441 1.17e-41 152
MS.gene003427.t1 AT3G12203 29.752 363 218 11 26 382 85 416 1.42e-41 151
MS.gene003427.t1 AT1G73270 28.463 397 231 14 2 382 82 441 1.44e-41 152
MS.gene003427.t1 AT2G23000 29.365 378 237 10 4 374 76 430 1.82e-41 151
MS.gene003427.t1 AT2G23000 29.365 378 237 10 4 374 76 430 1.82e-41 151
MS.gene003427.t1 AT2G22980 32.698 367 197 14 4 357 77 406 1.84e-41 150
MS.gene003427.t1 AT3G12203 29.752 363 218 11 26 382 106 437 2.47e-41 151
MS.gene003427.t1 AT2G22980 32.703 370 199 14 4 360 77 409 2.52e-41 151
MS.gene003427.t1 AT1G33540 28.500 400 254 13 2 382 35 421 5.02e-41 150
MS.gene003427.t1 AT1G73310 29.744 390 235 13 2 382 82 441 5.45e-41 150
MS.gene003427.t1 AT2G23010 29.708 377 237 11 4 374 76 430 8.84e-41 149
MS.gene003427.t1 AT1G33540 28.500 400 254 13 2 382 78 464 1.44e-40 149
MS.gene003427.t1 AT1G73280 28.969 359 227 10 26 382 7 339 3.69e-40 145
MS.gene003427.t1 AT5G36180 30.387 362 218 12 26 382 7 339 4.36e-40 145
MS.gene003427.t1 AT5G36180 30.387 362 218 12 26 382 7 339 4.36e-40 145
MS.gene003427.t1 AT2G23010 29.443 377 238 11 4 374 76 430 4.47e-40 147
MS.gene003427.t1 AT2G22990 28.947 342 217 11 43 382 2 319 1.09e-38 141
MS.gene003427.t1 AT2G22970 28.682 387 243 11 2 382 74 433 1.25e-38 144
MS.gene003427.t1 AT5G09640 29.630 405 246 16 2 382 76 465 1.49e-38 144
MS.gene003427.t1 AT2G22990 29.351 385 232 14 4 382 74 424 2.69e-38 142
MS.gene003427.t1 AT1G73290 29.769 346 212 10 4 345 83 401 3.07e-38 142
MS.gene003427.t1 AT3G10450 28.889 360 224 11 4 357 37 370 3.91e-38 141
MS.gene003427.t1 AT2G22990 28.655 342 218 11 43 382 2 319 4.36e-38 139
MS.gene003427.t1 AT3G10450 28.889 360 224 11 4 357 80 413 6.65e-38 141
MS.gene003427.t1 AT2G22920 30.682 352 211 11 2 346 74 399 7.94e-38 141
MS.gene003427.t1 AT5G36180 30.861 337 200 11 26 357 7 315 5.07e-37 137
MS.gene003427.t1 AT3G12220 30.311 386 234 13 4 382 48 405 6.46e-37 138
MS.gene003427.t1 AT3G12220 30.155 388 236 13 2 382 76 435 1.46e-36 138
MS.gene003427.t1 AT3G56540 60.952 105 39 2 4 108 130 232 1.49e-36 134
MS.gene003427.t1 AT2G22970 29.477 363 224 10 4 360 76 412 5.70e-36 136
MS.gene003427.t1 AT3G45010 29.323 399 228 14 2 380 135 499 1.10e-35 137
MS.gene003427.t1 AT1G73310 29.917 361 216 11 2 354 82 413 4.42e-35 134
MS.gene003427.t1 AT2G22970 29.006 362 225 10 2 357 74 409 4.52e-35 134
MS.gene003427.t1 AT2G22980 31.481 324 177 12 43 357 2 289 5.34e-35 130
MS.gene003427.t1 AT3G12240 28.682 387 243 14 2 382 77 436 6.69e-35 134
MS.gene003427.t1 AT1G73280 28.235 340 216 10 45 382 1 314 8.85e-35 130
MS.gene003427.t1 AT5G36180 30.938 320 189 10 26 341 7 298 5.21e-34 129
MS.gene003427.t1 AT3G12230 29.534 386 241 12 2 382 76 435 1.28e-33 130
MS.gene003427.t1 AT1G73310 30.030 333 201 9 26 354 7 311 1.89e-33 127
MS.gene003427.t1 AT5G22980 28.186 408 219 15 2 380 132 494 7.32e-31 123
MS.gene003427.t1 AT3G10410 27.877 391 240 15 2 379 133 494 2.95e-30 122
MS.gene003427.t1 AT2G22920 33.491 212 133 5 2 208 74 282 2.19e-26 108
MS.gene003427.t1 AT2G22980 34.434 212 124 6 4 208 77 280 3.86e-26 108
MS.gene003427.t1 AT1G73290 30.769 221 145 5 4 220 83 299 5.03e-26 107
MS.gene003427.t1 AT1G73280 31.336 217 141 5 4 215 84 297 7.62e-24 102
MS.gene003427.t1 AT2G22970 33.019 212 133 6 2 208 74 281 1.62e-23 100
MS.gene003427.t1 AT1G15000 26.020 392 241 15 2 382 73 426 8.65e-21 94.0
MS.gene003427.t1 AT3G12220 34.659 176 106 5 2 172 76 247 4.00e-20 91.3
MS.gene003427.t1 AT2G22920 25.483 259 165 9 128 382 22 256 1.35e-17 82.0
MS.gene003427.t1 AT2G22960 29.927 137 92 4 247 382 51 184 1.60e-15 74.7
MS.gene003427.t1 AT5G22960 38.776 98 51 3 38 135 83 171 2.73e-15 74.3

Find 88 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTAGGTTGGCTGCTATTT+TGG 0.094675 4.1:+28972137 None:intergenic
CCACCTTTATTCCAAGAATA+TGG 0.134085 4.1:+28972103 None:intergenic
AAGATACACATTAAGAAAAT+TGG 0.169206 4.1:-28969744 MS.gene003427:CDS
ATTGATGAAACTCACAAATT+TGG 0.172617 4.1:+28971024 None:intergenic
ACAACTTTCTGAGCTCATTT+TGG 0.173222 4.1:-28971469 MS.gene003427:CDS
TTGCTGGTGGTATTCGTATA+TGG 0.207100 4.1:-28970078 MS.gene003427:CDS
GTGTATCTTGTTGCAGTTAC+TGG 0.229082 4.1:+28969758 None:intergenic
TTTGAACCTCTGGCTTATTT+AGG 0.237983 4.1:+28970360 None:intergenic
TGAAGTTAATGTAATCTTCT+TGG 0.258139 4.1:+28971427 None:intergenic
TCAAGTTGAACCCATATTCT+TGG 0.308306 4.1:-28972114 MS.gene003427:CDS
AACTAGCTGAAGAGCTTGTT+TGG 0.310257 4.1:+28969086 None:intergenic
AACCCATATTCTTGGAATAA+AGG 0.317209 4.1:-28972106 MS.gene003427:intron
TTAGTAATAATATAACATTT+TGG 0.318343 4.1:-28970144 MS.gene003427:intron
TAAGAGATGAGTAATTAAAT+AGG 0.322867 4.1:+28969027 None:intergenic
CACACACATTCCTCATCAAT+TGG 0.333373 4.1:-28971647 MS.gene003427:CDS
TGCTGGTGGTATTCGTATAT+GGG 0.345254 4.1:-28970077 MS.gene003427:CDS
GGCCCAGGTTGTTCTTCAAT+TGG 0.348842 4.1:-28972196 MS.gene003427:CDS
CCTATCCATGGACTCATTGC+AGG 0.365800 4.1:-28970307 MS.gene003427:intron
TCACATGATTTCTACATTGC+TGG 0.366413 4.1:-28971600 MS.gene003427:CDS
CACCTTTATTCCAAGAATAT+GGG 0.373339 4.1:+28972104 None:intergenic
GAAGTTAATGTAATCTTCTT+GGG 0.378539 4.1:+28971428 None:intergenic
TTGATGACCAGCACCTCTTA+TGG 0.386736 4.1:+28969125 None:intergenic
GATGATGAAACAGATCAAAA+AGG 0.393118 4.1:-28971274 MS.gene003427:CDS
GAACTTGGTGACACCATTAC+AGG 0.404445 4.1:-28971775 MS.gene003427:intron
GTCCTCAAGAAGCTTATTGC+TGG 0.405861 4.1:-28970094 MS.gene003427:CDS
TGCATATGGTTGTGATGTTA+TGG 0.409977 4.1:+28971203 None:intergenic
GTATTCGTATATGGGTTTAC+AGG 0.411990 4.1:-28970069 MS.gene003427:intron
GGGGTCCAATCTTGAACTAT+AGG 0.417940 4.1:+28969716 None:intergenic
AGAATTGTTGTTACCTGTAA+TGG 0.419485 4.1:+28971762 None:intergenic
CTTGAATCCCCTGTTGGAGT+GGG 0.424871 4.1:-28971832 MS.gene003427:CDS
GACTCCCTATAGTTCAAGAT+TGG 0.427044 4.1:-28969721 MS.gene003427:CDS
CATTAACTTCAAAGGGATCA+TGG 0.434911 4.1:-28971415 MS.gene003427:intron
TATGGGTTCAACTTGAGCTT+AGG 0.436146 4.1:+28972121 None:intergenic
CTAACATTTGTGACCATAAG+AGG 0.440806 4.1:-28969138 MS.gene003427:CDS
AAGATTACATTAACTTCAAA+GGG 0.442517 4.1:-28971422 MS.gene003427:CDS
TGAAATTATAGGTGGGAGGA+TGG 0.448499 4.1:-28969179 MS.gene003427:intron
TTGTTTCTTGAATCCCCTGT+TGG 0.451158 4.1:-28971838 MS.gene003427:CDS
AAATCATGTGATTTGAATTG+AGG 0.451666 4.1:+28971612 None:intergenic
GAAGATTACATTAACTTCAA+AGG 0.454184 4.1:-28971423 MS.gene003427:CDS
AGATACACATTAAGAAAATT+GGG 0.455034 4.1:-28969743 MS.gene003427:CDS
GGGTCCAATCTTGAACTATA+GGG 0.463490 4.1:+28969717 None:intergenic
GGTTGTTCTTCAATTGGCTA+TGG 0.471699 4.1:-28972190 MS.gene003427:CDS
GGTTCAACTTGAGCTTAGGT+TGG 0.479507 4.1:+28972125 None:intergenic
ACTAGCTGAAGAGCTTGTTT+GGG 0.480171 4.1:+28969087 None:intergenic
GCTTGTTTGGGAGCAAAAGT+AGG 0.508439 4.1:+28969099 None:intergenic
AAATAAGCCAGAGGTTCAAA+AGG 0.512103 4.1:-28970357 MS.gene003427:CDS
TCATTATTATAGTGGAGATA+CGG 0.513963 4.1:-28969792 MS.gene003427:intron
AAGGTATGATAGAGTATGCA+TGG 0.516188 4.1:-28971255 MS.gene003427:CDS
ACCAGTGATATACGTGAACT+TGG 0.523134 4.1:-28971790 MS.gene003427:CDS
GCAATAAGCTTCTTGAGGAC+AGG 0.532175 4.1:+28970097 None:intergenic
TGGATGGCTGAAGTTGCATA+TGG 0.532733 4.1:+28971189 None:intergenic
CTGCAATGAGTCCATGGATA+GGG 0.534961 4.1:+28970308 None:intergenic
TATGGAGAAGCAGAAGAACT+TGG 0.539974 4.1:-28972172 MS.gene003427:CDS
GCAACATCAGCAATGTCAGA+AGG 0.545792 4.1:-28971066 MS.gene003427:CDS
CTTCTTGAGGACAGGAAGCA+TGG 0.550587 4.1:+28970105 None:intergenic
AGTAATTAAATAGGATGTTG+TGG 0.551015 4.1:+28969036 None:intergenic
ACATACCTGCAATGAGTCCA+TGG 0.552603 4.1:+28970302 None:intergenic
TAGCCAATTGAAGAACAACC+TGG 0.552876 4.1:+28972193 None:intergenic
TCTTGAATCCCCTGTTGGAG+TGG 0.554368 4.1:-28971833 MS.gene003427:CDS
TTTGTGACCATAAGAGGTGC+TGG 0.556501 4.1:-28969132 MS.gene003427:CDS
TAGTTCAAGATTGGACCCCT+TGG 0.566312 4.1:-28969712 MS.gene003427:CDS
ACCAAGTTCACGTATATCAC+TGG 0.571029 4.1:+28971789 None:intergenic
CCCTTGGTATACTAGTCGCC+AGG 0.572862 4.1:-28969696 MS.gene003427:intron
ACGTATATCACTGGTGGTGT+TGG 0.576540 4.1:+28971798 None:intergenic
GTCAAATTTAACAGGACGGG+TGG 0.583653 4.1:-28970436 MS.gene003427:intron
CTCAAGAAGCTTATTGCTGG+TGG 0.589445 4.1:-28970091 MS.gene003427:CDS
CAACATCAGCAATGTCAGAA+GGG 0.602739 4.1:-28971065 MS.gene003427:CDS
CCTGCAATGAGTCCATGGAT+AGG 0.606205 4.1:+28970307 None:intergenic
TATTATAGTGGAGATACGGA+TGG 0.610797 4.1:-28969788 MS.gene003427:intron
GCTGATGTTGCTGAAACACA+TGG 0.619342 4.1:+28971078 None:intergenic
ATTGCTGGTGAAAGCTACGC+AGG 0.625309 4.1:-28971585 MS.gene003427:intron
AGGTATGATAGAGTATGCAT+GGG 0.627519 4.1:-28971254 MS.gene003427:CDS
GGATGGAACATTGTATATGA+CGG 0.632023 4.1:-28969162 MS.gene003427:CDS
CACCAGCAATAAGCTTCTTG+AGG 0.633817 4.1:+28970092 None:intergenic
ATCACTGGTGGTGTTGGTGT+AGG 0.635134 4.1:+28971804 None:intergenic
AAAATGAGCTCAGAAAGTTG+TGG 0.642584 4.1:+28971471 None:intergenic
AGCCAATTGAAGAACAACCT+GGG 0.652832 4.1:+28972194 None:intergenic
ACCTGGCGACTAGTATACCA+AGG 0.654015 4.1:+28969695 None:intergenic
CCTGGCGACTAGTATACCAA+GGG 0.655244 4.1:+28969696 None:intergenic
GACCATGCTGTAATATCTGA+TGG 0.667336 4.1:-28971232 MS.gene003427:CDS
AACCATCAGATATTACAGCA+TGG 0.672003 4.1:+28971230 None:intergenic
AGGACAGGAAGCATGGACAC+AGG 0.674862 4.1:+28970112 None:intergenic
GCAGTATAATCTGATGCACA+TGG 0.677312 4.1:+28970389 None:intergenic
GGTATGAAATTATAGGTGGG+AGG 0.687576 4.1:-28969183 MS.gene003427:intron
AAGTTCACGTATATCACTGG+TGG 0.693723 4.1:+28971792 None:intergenic
AGCATACCTAAATAAGCCAG+AGG 0.700291 4.1:-28970366 MS.gene003427:CDS
CTGATGTTGCTGAAACACAT+GGG 0.718323 4.1:+28971079 None:intergenic
CTGGCGACTAGTATACCAAG+GGG 0.757863 4.1:+28969697 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATACTATTAATAAAACA+TGG + chr4.1:28971415-28971434 None:intergenic 10.0%
!! AAAATGTTATATTATTACTA+AGG + chr4.1:28972018-28972037 None:intergenic 10.0%
!! ATAATAAGAAGAAAAAAAAA+TGG + chr4.1:28972916-28972935 None:intergenic 10.0%
!! TGTCTAATTAATATTAATAT+TGG + chr4.1:28970829-28970848 None:intergenic 10.0%
!!! AATATGTTTATAGTAATTTT+TGG - chr4.1:28972210-28972229 MS.gene003427:intron 10.0%
!!! TTAGTAATAATATAACATTT+TGG - chr4.1:28972017-28972036 MS.gene003427:intron 10.0%
!! TAAAAAAATGTTTCATAAAG+TGG + chr4.1:28972953-28972972 None:intergenic 15.0%
!! TAAATATGTATTAATGTTTC+AGG - chr4.1:28969944-28969963 MS.gene003427:intron 15.0%
!!! AAAAGAATTTAAAAGTACTT+AGG - chr4.1:28969837-28969856 MS.gene003427:intron 15.0%
!!! AAACAAAAATTACAATTTTG+AGG + chr4.1:28972856-28972875 None:intergenic 15.0%
!!! AATGAATTTTTTTATAGAAG+TGG + chr4.1:28969561-28969580 None:intergenic 15.0%
!!! ACTCAAATTAATTTAAAGTT+AGG + chr4.1:28970642-28970661 None:intergenic 15.0%
!!! ATGAAACATTTTTTTATTTG+TGG - chr4.1:28972957-28972976 MS.gene003427:intron 15.0%
!!! CTTTATTTTCATTATTATAG+TGG - chr4.1:28972361-28972380 MS.gene003427:intron 15.0%
!!! TCTTTTTTTTTATTAGCTAT+TGG + chr4.1:28969823-28969842 None:intergenic 15.0%
!! AAGATACACATTAAGAAAAT+TGG - chr4.1:28972417-28972436 MS.gene003427:intron 20.0%
!! AAGATTACATTAACTTCAAA+GGG - chr4.1:28970739-28970758 MS.gene003427:intron 20.0%
!! AATTGGGCATTTAATTAAAT+TGG - chr4.1:28969140-28969159 MS.gene003427:CDS 20.0%
!! AGAAATAGAACTAATAATGT+TGG - chr4.1:28972705-28972724 MS.gene003427:intron 20.0%
!! AGATACACATTAAGAAAATT+GGG - chr4.1:28972418-28972437 MS.gene003427:intron 20.0%
!! ATAAAGTAGTAATCATGAAT+TGG - chr4.1:28969372-28969391 MS.gene003427:intron 20.0%
!! CGTCTATTAGTTATTATATA+CGG + chr4.1:28971441-28971460 None:intergenic 20.0%
!! TATTTGTGGTATGAAATTAT+AGG - chr4.1:28972971-28972990 MS.gene003427:intron 20.0%
!! TTCATGATTACTACTTTATT+AGG + chr4.1:28969371-28969390 None:intergenic 20.0%
!! TTGAAATTGTCAAATTTAAC+AGG - chr4.1:28971717-28971736 MS.gene003427:intron 20.0%
!!! AAGAATATAAGTTGAAGTTA+AGG + chr4.1:28972639-28972658 None:intergenic 20.0%
!!! AGAATATAAGTTGAAGTTAA+GGG + chr4.1:28972638-28972657 None:intergenic 20.0%
!!! CTAGTTTTTAAGTTCTAAAT+TGG + chr4.1:28971173-28971192 None:intergenic 20.0%
!!! GATATTTTATGTAGAATTCT+TGG - chr4.1:28969730-28969749 MS.gene003427:CDS 20.0%
!!! TTTTTAAGTTCTAAATTGGA+AGG + chr4.1:28971169-28971188 None:intergenic 20.0%
! AAAACAATAGAGAGAGAAAA+AGG + chr4.1:28971596-28971615 None:intergenic 25.0%
! AAATCATGTGATTTGAATTG+AGG + chr4.1:28970552-28970571 None:intergenic 25.0%
! AAATTTGGAAAAAGAATGAG+GGG + chr4.1:28971125-28971144 None:intergenic 25.0%
! AATTGTCAAATTTAACAGGA+CGG - chr4.1:28971721-28971740 MS.gene003427:intron 25.0%
! ACAAATTTGGAAAAAGAATG+AGG + chr4.1:28971127-28971146 None:intergenic 25.0%
! AGGGTTAACAAAAAAAAAAG+TGG - chr4.1:28972787-28972806 MS.gene003427:intron 25.0%
! ATTAGACACAATGAAATGTT+TGG - chr4.1:28970841-28970860 MS.gene003427:intron 25.0%
! ATTGATGAAACTCACAAATT+TGG + chr4.1:28971140-28971159 None:intergenic 25.0%
! CAAATTTGGAAAAAGAATGA+GGG + chr4.1:28971126-28971145 None:intergenic 25.0%
! CAACAATTCTATATTGTCTA+AGG - chr4.1:28970412-28970431 MS.gene003427:CDS 25.0%
! GAAGATTACATTAACTTCAA+AGG - chr4.1:28970738-28970757 MS.gene003427:intron 25.0%
! GAAGTTAATGTAATCTTCTT+GGG + chr4.1:28970736-28970755 None:intergenic 25.0%
! GTACAAAAGTTGTATCAAAT+TGG + chr4.1:28971529-28971548 None:intergenic 25.0%
! TAAATCAACTTAGAACAACT+TGG + chr4.1:28972495-28972514 None:intergenic 25.0%
! TAATATACAACTTCTACTTG+AGG - chr4.1:28972604-28972623 MS.gene003427:intron 25.0%
! TCTTAAAGGTTGTAACAAAA+TGG - chr4.1:28971647-28971666 MS.gene003427:CDS 25.0%
! TGAAGTTAATGTAATCTTCT+TGG + chr4.1:28970737-28970756 None:intergenic 25.0%
! TGAGTTTGTATTGAAATTTC+AGG - chr4.1:28970657-28970676 MS.gene003427:intron 25.0%
!! ACAGATTGGAAATGTTTTAT+TGG - chr4.1:28970865-28970884 MS.gene003427:intron 25.0%
!! ATCTTTTAAACCAATTGATG+AGG + chr4.1:28970527-28970546 None:intergenic 25.0%
!! GAATATAAGTTGAAGTTAAG+GGG + chr4.1:28972637-28972656 None:intergenic 25.0%
!! TCATTATTATAGTGGAGATA+CGG - chr4.1:28972369-28972388 MS.gene003427:intron 25.0%
!!! GATTAGTCTATTTTTTACGA+AGG + chr4.1:28972148-28972167 None:intergenic 25.0%
!!! TCATCTGTTTGATTTTGAAT+TGG + chr4.1:28970995-28971014 None:intergenic 25.0%
!!! TGTTTTAACATGAATTCATG+TGG - chr4.1:28972871-28972890 MS.gene003427:intron 25.0%
AACCCATATTCTTGGAATAA+AGG - chr4.1:28970055-28970074 MS.gene003427:intron 30.0%
AAGAGAATTAGACTTACGTA+TGG - chr4.1:28969619-28969638 MS.gene003427:intron 30.0%
AATCATGAATTGGACTTACT+TGG - chr4.1:28969382-28969401 MS.gene003427:intron 30.0%
AGAGAAATATAGCAAACGTA+TGG + chr4.1:28972246-28972265 None:intergenic 30.0%
AGTTGCAAATGTACCTTATT+TGG + chr4.1:28971240-28971259 None:intergenic 30.0%
ATCATGAATTGGACTTACTT+GGG - chr4.1:28969383-28969402 MS.gene003427:intron 30.0%
ATGAAATGTTTGGTACAGAT+TGG - chr4.1:28970851-28970870 MS.gene003427:intron 30.0%
ATTGTCAAATTTAACAGGAC+GGG - chr4.1:28971722-28971741 MS.gene003427:intron 30.0%
ATTGTGAAAAGATGAAAAGC+TGG + chr4.1:28969432-28969451 None:intergenic 30.0%
CACCTTTATTCCAAGAATAT+GGG + chr4.1:28970060-28970079 None:intergenic 30.0%
CACTTAAACCAATTCATCAT+TGG + chr4.1:28969597-28969616 None:intergenic 30.0%
CATAAAGTGGAGAGTAAAAT+TGG + chr4.1:28972940-28972959 None:intergenic 30.0%
CATATTCTTGGAATAAAGGT+GGG - chr4.1:28970059-28970078 MS.gene003427:intron 30.0%
GAACAACAAAGTTGTCTTAA+AGG - chr4.1:28971633-28971652 MS.gene003427:CDS 30.0%
GATGATGAAACAGATCAAAA+AGG - chr4.1:28970887-28970906 MS.gene003427:intron 30.0%
TGTGGTATGAAATTATAGGT+GGG - chr4.1:28972975-28972994 MS.gene003427:intron 30.0%
TTGTGAAAAGATGAAAAGCT+GGG + chr4.1:28969431-28969450 None:intergenic 30.0%
TTGTGGTATGAAATTATAGG+TGG - chr4.1:28972974-28972993 MS.gene003427:intron 30.0%
TTTATTTCATTGACCAGCTA+AGG - chr4.1:28970488-28970507 MS.gene003427:intron 30.0%
TTTGCTTACCAATGATGAAT+TGG - chr4.1:28969586-28969605 MS.gene003427:intron 30.0%
! AGAATTGTTGTTACCTGTAA+TGG + chr4.1:28970402-28970421 None:intergenic 30.0%
! AGCAATACGAATTGATAACT+AGG - chr4.1:28969892-28969911 MS.gene003427:intron 30.0%
! AGTTAGTTAGTTGTAGCTTT+TGG - chr4.1:28969108-28969127 MS.gene003427:CDS 30.0%
! TAGAATTCTTGGTTTGAATC+CGG - chr4.1:28969741-28969760 MS.gene003427:CDS 30.0%
! TCAATGATCTAATCGACTTA+AGG + chr4.1:28969316-28969335 None:intergenic 30.0%
!! CTGTTTGATTTTGAATTGGA+TGG + chr4.1:28970991-28971010 None:intergenic 30.0%
!! TGTTTTCTCTAAGAATCAGA+GGG - chr4.1:28972768-28972787 MS.gene003427:intron 30.0%
!! TTGTTGTTCTCTCTTTTCAT+TGG + chr4.1:28971622-28971641 None:intergenic 30.0%
!!! CTTTTGGTTTGTGCTTAATT+GGG - chr4.1:28969124-28969143 MS.gene003427:CDS 30.0%
AAAATGAGCTCAGAAAGTTG+TGG + chr4.1:28970693-28970712 None:intergenic 35.0%
AAATAAGCCAGAGGTTCAAA+AGG - chr4.1:28971804-28971823 MS.gene003427:CDS 35.0%
AACCATCAGATATTACAGCA+TGG + chr4.1:28970934-28970953 None:intergenic 35.0%
AAGGTATGATAGAGTATGCA+TGG - chr4.1:28970906-28970925 MS.gene003427:intron 35.0%
AATCATGTCCACTAAATTGC+CGG - chr4.1:28969285-28969304 MS.gene003427:intron 35.0%
ACTAATTAGCGTTACTGTTG+TGG - chr4.1:28969695-28969714 MS.gene003427:intron 35.0%
AGAAGTGGATCAAGTAGTTA+CGG + chr4.1:28969546-28969565 None:intergenic 35.0%
AGCTTAAAGAGTCATTGCAT+AGG - chr4.1:28969862-28969881 MS.gene003427:intron 35.0%
AGGTATGATAGAGTATGCAT+GGG - chr4.1:28970907-28970926 MS.gene003427:intron 35.0%
AGTTACGGGTAATAATCACT+TGG + chr4.1:28969531-28969550 None:intergenic 35.0%
CAATAGTGCACTACCAAATA+AGG - chr4.1:28971224-28971243 MS.gene003427:CDS 35.0%
CATTAACTTCAAAGGGATCA+TGG - chr4.1:28970746-28970765 MS.gene003427:intron 35.0%
CCACCTTTATTCCAAGAATA+TGG + chr4.1:28970061-28970080 None:intergenic 35.0%
CCATATTCTTGGAATAAAGG+TGG - chr4.1:28970058-28970077 MS.gene003427:intron 35.0%
GGATGGAACATTGTATATGA+CGG - chr4.1:28972999-28973018 MS.gene003427:intron 35.0%
GGGTGTGTTTCTTTGAATAT+GGG - chr4.1:28969785-28969804 MS.gene003427:intron 35.0%
GTATTCGTATATGGGTTTAC+AGG - chr4.1:28972092-28972111 MS.gene003427:intron 35.0%
GTGTTTCTTTGAATATGGGT+TGG - chr4.1:28969789-28969808 MS.gene003427:intron 35.0%
TACTAGTTGTGCATTTCACT+AGG + chr4.1:28971274-28971293 None:intergenic 35.0%
TCACAAAAATTCCCTATCCA+TGG - chr4.1:28971842-28971861 MS.gene003427:CDS 35.0%
TCACATGATTTCTACATTGC+TGG - chr4.1:28970561-28970580 MS.gene003427:intron 35.0%
TGCATATGGTTGTGATGTTA+TGG + chr4.1:28970961-28970980 None:intergenic 35.0%
TTAGAACAACTTGGTTTACC+TGG + chr4.1:28972486-28972505 None:intergenic 35.0%
TTGCAGCTGTAGAAATCATA+TGG + chr4.1:28970298-28970317 None:intergenic 35.0%
TTGCTGAGAATTTCCAACTT+CGG + chr4.1:28969490-28969509 None:intergenic 35.0%
! ACAACTTTCTGAGCTCATTT+TGG - chr4.1:28970692-28970711 MS.gene003427:intron 35.0%
! TAATCGACTTAAGGAATAGC+CGG + chr4.1:28969307-28969326 None:intergenic 35.0%
! TATTATAGTGGAGATACGGA+TGG - chr4.1:28972373-28972392 MS.gene003427:intron 35.0%
! TTTGAACCTCTGGCTTATTT+AGG + chr4.1:28971804-28971823 None:intergenic 35.0%
!! ATGAAAAGCTGGGTTTTATG+TGG + chr4.1:28969421-28969440 None:intergenic 35.0%
!! CTGTTTTCTCTAAGAATCAG+AGG - chr4.1:28972767-28972786 MS.gene003427:intron 35.0%
!! TCAAGTTGAACCCATATTCT+TGG - chr4.1:28970047-28970066 MS.gene003427:intron 35.0%
!!! GCTTTTGGTTTGTGCTTAAT+TGG - chr4.1:28969123-28969142 MS.gene003427:CDS 35.0%
AAGTTCACGTATATCACTGG+TGG + chr4.1:28970372-28970391 None:intergenic 40.0%
AATGTGTGTGAATCCTTAGC+TGG + chr4.1:28970504-28970523 None:intergenic 40.0%
ACCAAGTTCACGTATATCAC+TGG + chr4.1:28970375-28970394 None:intergenic 40.0%
ACCAGTGATATACGTGAACT+TGG - chr4.1:28970371-28970390 MS.gene003427:CDS 40.0%
AGCATACCTAAATAAGCCAG+AGG - chr4.1:28971795-28971814 MS.gene003427:CDS 40.0%
AGCCAATTGAAGAACAACCT+GGG + chr4.1:28969970-28969989 None:intergenic 40.0%
CAACATCAGCAATGTCAGAA+GGG - chr4.1:28971096-28971115 MS.gene003427:CDS 40.0%
CACACACATTCCTCATCAAT+TGG - chr4.1:28970514-28970533 MS.gene003427:intron 40.0%
CGAAAGTGGTGAATGAACTT+TGG - chr4.1:28972735-28972754 MS.gene003427:intron 40.0%
CGGGTGTGTTTCTTTGAATA+TGG - chr4.1:28969784-28969803 MS.gene003427:intron 40.0%
CTGATGTTGCTGAAACACAT+GGG + chr4.1:28971085-28971104 None:intergenic 40.0%
GAAGTGGATCAAGTAGTTAC+GGG + chr4.1:28969545-28969564 None:intergenic 40.0%
GACCATGCTGTAATATCTGA+TGG - chr4.1:28970929-28970948 MS.gene003427:intron 40.0%
GACTCCCTATAGTTCAAGAT+TGG - chr4.1:28972440-28972459 MS.gene003427:intron 40.0%
GCAGTATAATCTGATGCACA+TGG + chr4.1:28971775-28971794 None:intergenic 40.0%
GGGTCCAATCTTGAACTATA+GGG + chr4.1:28972447-28972466 None:intergenic 40.0%
GGTATGAAATTATAGGTGGG+AGG - chr4.1:28972978-28972997 MS.gene003427:intron 40.0%
GGTTGTTCTTCAATTGGCTA+TGG - chr4.1:28969971-28969990 MS.gene003427:intron 40.0%
GTGTATCTTGTTGCAGTTAC+TGG + chr4.1:28972406-28972425 None:intergenic 40.0%
TAGCCAATTGAAGAACAACC+TGG + chr4.1:28969971-28969990 None:intergenic 40.0%
TATGGAGAAGCAGAAGAACT+TGG - chr4.1:28969989-28970008 MS.gene003427:intron 40.0%
TATGGGTTCAACTTGAGCTT+AGG + chr4.1:28970043-28970062 None:intergenic 40.0%
TGAAATTATAGGTGGGAGGA+TGG - chr4.1:28972982-28973001 MS.gene003427:intron 40.0%
TGCTGAGAATTTCCAACTTC+GGG + chr4.1:28969489-28969508 None:intergenic 40.0%
TGCTGGTGGTATTCGTATAT+GGG - chr4.1:28972084-28972103 MS.gene003427:intron 40.0%
TGTATTAATGTTTCAGGCCC+AGG - chr4.1:28969950-28969969 MS.gene003427:intron 40.0%
TTGCTGGTGGTATTCGTATA+TGG - chr4.1:28972083-28972102 MS.gene003427:intron 40.0%
! AACTAGCTGAAGAGCTTGTT+TGG + chr4.1:28973078-28973097 None:intergenic 40.0%
! ACTAGCTGAAGAGCTTGTTT+GGG + chr4.1:28973077-28973096 None:intergenic 40.0%
! TCACCACATTTTCTGCTGTT+CGG - chr4.1:28969764-28969783 MS.gene003427:CDS 40.0%
! TTGTTTCTTGAATCCCCTGT+TGG - chr4.1:28970323-28970342 MS.gene003427:CDS 40.0%
!! ACTGTTGTGGTTTGTTTGTG+AGG - chr4.1:28969708-28969727 MS.gene003427:CDS 40.0%
!!! CTGCTATTTTGGGGGAAAAA+GGG + chr4.1:28970016-28970035 None:intergenic 40.0%
!!! CTTAGGTTGGCTGCTATTTT+GGG + chr4.1:28970026-28970045 None:intergenic 40.0%
!!! TTAGGTTGGCTGCTATTTTG+GGG + chr4.1:28970025-28970044 None:intergenic 40.0%
ACAGCAGAAAATGTGGTGAC+CGG + chr4.1:28969763-28969782 None:intergenic 45.0%
ACATACCTGCAATGAGTCCA+TGG + chr4.1:28971862-28971881 None:intergenic 45.0%
AGGAAAAACCCACTCCAACA+GGG + chr4.1:28970340-28970359 None:intergenic 45.0%
AGGAATAGCCGGCAATTTAG+TGG + chr4.1:28969296-28969315 None:intergenic 45.0%
CACCAGCAATAAGCTTCTTG+AGG + chr4.1:28972072-28972091 None:intergenic 45.0%
CTCAAGAAGCTTATTGCTGG+TGG - chr4.1:28972070-28972089 MS.gene003427:intron 45.0%
CTGCAATGAGTCCATGGATA+GGG + chr4.1:28971856-28971875 None:intergenic 45.0%
GCAACATCAGCAATGTCAGA+AGG - chr4.1:28971095-28971114 MS.gene003427:CDS 45.0%
GCAATAAGCTTCTTGAGGAC+AGG + chr4.1:28972067-28972086 None:intergenic 45.0%
GCTGATGTTGCTGAAACACA+TGG + chr4.1:28971086-28971105 None:intergenic 45.0%
GCTTGTTTGGGAGCAAAAGT+AGG + chr4.1:28973065-28973084 None:intergenic 45.0%
GGGGTCCAATCTTGAACTAT+AGG + chr4.1:28972448-28972467 None:intergenic 45.0%
GGGTGGCATAAAAATCTAGC+AGG - chr4.1:28971742-28971761 MS.gene003427:intron 45.0%
GGTTCAACTTGAGCTTAGGT+TGG + chr4.1:28970039-28970058 None:intergenic 45.0%
GTCAAATTTAACAGGACGGG+TGG - chr4.1:28971725-28971744 MS.gene003427:intron 45.0%
GTCCTCAAGAAGCTTATTGC+TGG - chr4.1:28972067-28972086 MS.gene003427:intron 45.0%
TAGGAAAAACCCACTCCAAC+AGG + chr4.1:28970341-28970360 None:intergenic 45.0%
TAGTTCAAGATTGGACCCCT+TGG - chr4.1:28972449-28972468 MS.gene003427:intron 45.0%
TGGATGGCTGAAGTTGCATA+TGG + chr4.1:28970975-28970994 None:intergenic 45.0%
! ACGTATATCACTGGTGGTGT+TGG + chr4.1:28970366-28970385 None:intergenic 45.0%
! CACCACATTTTCTGCTGTTC+GGG - chr4.1:28969765-28969784 MS.gene003427:CDS 45.0%
! GAACTTGGTGACACCATTAC+AGG - chr4.1:28970386-28970405 MS.gene003427:CDS 45.0%
!! ATGTTGGCTCAATGCGAAAG+TGG - chr4.1:28972721-28972740 MS.gene003427:intron 45.0%
!! TGAAGTGCCTTTTGAACCTC+TGG + chr4.1:28971814-28971833 None:intergenic 45.0%
!!! GCTGCTATTTTGGGGGAAAA+AGG + chr4.1:28970017-28970036 None:intergenic 45.0%
!!! GCTTAGGTTGGCTGCTATTT+TGG + chr4.1:28970027-28970046 None:intergenic 45.0%
!!! TAGGTTGGCTGCTATTTTGG+GGG + chr4.1:28970024-28970043 None:intergenic 45.0%
!!! TAAATAATTTTTTTATAAGT+TGG - chr4.1:28972274-28972293 MS.gene003427:intron 5.0%
ACCTGGCGACTAGTATACCA+AGG + chr4.1:28972469-28972488 None:intergenic 50.0%
AGTCTACGCAATCCCGAAGT+TGG - chr4.1:28969474-28969493 MS.gene003427:intron 50.0%
ATTGCTGGTGAAAGCTACGC+AGG - chr4.1:28970576-28970595 MS.gene003427:intron 50.0%
CACCCGAACAGCAGAAAATG+TGG + chr4.1:28969770-28969789 None:intergenic 50.0%
CCTATCCATGGACTCATTGC+AGG - chr4.1:28971854-28971873 MS.gene003427:intron 50.0%
CCTGCAATGAGTCCATGGAT+AGG + chr4.1:28971857-28971876 None:intergenic 50.0%
CCTGGCGACTAGTATACCAA+GGG + chr4.1:28972468-28972487 None:intergenic 50.0%
CTGGCGACTAGTATACCAAG+GGG + chr4.1:28972467-28972486 None:intergenic 50.0%
CTTCTTGAGGACAGGAAGCA+TGG + chr4.1:28972059-28972078 None:intergenic 50.0%
CTTGAATCCCCTGTTGGAGT+GGG - chr4.1:28970329-28970348 MS.gene003427:CDS 50.0%
GGAAAAACCCACTCCAACAG+GGG + chr4.1:28970339-28970358 None:intergenic 50.0%
GGCCCAGGTTGTTCTTCAAT+TGG - chr4.1:28969965-28969984 MS.gene003427:intron 50.0%
TCTTGAATCCCCTGTTGGAG+TGG - chr4.1:28970328-28970347 MS.gene003427:CDS 50.0%
!! ATCACTGGTGGTGTTGGTGT+AGG + chr4.1:28970360-28970379 None:intergenic 50.0%
CCCTTGGTATACTAGTCGCC+AGG - chr4.1:28972465-28972484 MS.gene003427:intron 55.0%
! AGGACAGGAAGCATGGACAC+AGG + chr4.1:28972052-28972071 None:intergenic 55.0%
CGCTGACATGTGACGCAACG+TGG - chr4.1:28971459-28971478 MS.gene003427:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 28969041 28973142 28969041 ID=MS.gene003427
chr4.1 mRNA 28969041 28973142 28969041 ID=MS.gene003427.t1;Parent=MS.gene003427
chr4.1 exon 28973130 28973142 28973130 ID=MS.gene003427.t1.exon1;Parent=MS.gene003427.t1
chr4.1 CDS 28973130 28973142 28973130 ID=cds.MS.gene003427.t1;Parent=MS.gene003427.t1
chr4.1 exon 28972107 28972217 28972107 ID=MS.gene003427.t1.exon2;Parent=MS.gene003427.t1
chr4.1 CDS 28972107 28972217 28972107 ID=cds.MS.gene003427.t1;Parent=MS.gene003427.t1
chr4.1 exon 28971776 28971871 28971776 ID=MS.gene003427.t1.exon3;Parent=MS.gene003427.t1
chr4.1 CDS 28971776 28971871 28971776 ID=cds.MS.gene003427.t1;Parent=MS.gene003427.t1
chr4.1 exon 28971586 28971678 28971586 ID=MS.gene003427.t1.exon4;Parent=MS.gene003427.t1
chr4.1 CDS 28971586 28971678 28971586 ID=cds.MS.gene003427.t1;Parent=MS.gene003427.t1
chr4.1 exon 28971416 28971504 28971416 ID=MS.gene003427.t1.exon5;Parent=MS.gene003427.t1
chr4.1 CDS 28971416 28971504 28971416 ID=cds.MS.gene003427.t1;Parent=MS.gene003427.t1
chr4.1 exon 28971039 28971314 28971039 ID=MS.gene003427.t1.exon6;Parent=MS.gene003427.t1
chr4.1 CDS 28971039 28971314 28971039 ID=cds.MS.gene003427.t1;Parent=MS.gene003427.t1
chr4.1 exon 28970308 28970444 28970308 ID=MS.gene003427.t1.exon7;Parent=MS.gene003427.t1
chr4.1 CDS 28970308 28970444 28970308 ID=cds.MS.gene003427.t1;Parent=MS.gene003427.t1
chr4.1 exon 28970070 28970162 28970070 ID=MS.gene003427.t1.exon8;Parent=MS.gene003427.t1
chr4.1 CDS 28970070 28970162 28970070 ID=cds.MS.gene003427.t1;Parent=MS.gene003427.t1
chr4.1 exon 28969697 28969802 28969697 ID=MS.gene003427.t1.exon9;Parent=MS.gene003427.t1
chr4.1 CDS 28969697 28969802 28969697 ID=cds.MS.gene003427.t1;Parent=MS.gene003427.t1
chr4.1 exon 28969041 28969190 28969041 ID=MS.gene003427.t1.exon10;Parent=MS.gene003427.t1
chr4.1 CDS 28969041 28969190 28969041 ID=cds.MS.gene003427.t1;Parent=MS.gene003427.t1
Gene Sequence

>MS.gene003427

ATGATGCTAGCTAGTAAGCTTTTAGTCTCAAAATTTCTCTAATTTGTAAACTTTATGTCATTTTCAAAGTTAGTTAGTTGTAGCTTTTGGTTTGTGCTTAATTGGGCATTTAATTAAATTGGTCTTTCTAATCATAGTTTGAACTTTGAACAAAAGTTAGAAAGACAAAATTGCAATCAAATTGTAGTGTGTAGACATTTTTTTTTTTTTGAGAGAGTGTGTAGACATTTTAGTCATCAAGTTTAATCATGTCCACTAAATTGCCGGCTATTCCTTAAGTCGATTAGATCATTGATTAAAAATGCTTTGAATCACATAAGTCATTCTCCTAATAAAGTAGTAATCATGAATTGGACTTACTTGGGCAACATGACGTGCCACATAAAACCCAGCTTTTCATCTTTTCACAATAAAAAAAATTCAGCTTTTGATGAGTCTACGCAATCCCGAAGTTGGAAATTCTCAGCAAAATGATATCATTCAAGTTCCAAGTGATTATTACCCGTAACTACTTGATCCACTTCTATAAAAAAATTCATTAAAGTTTTGCTTACCAATGATGAATTGGTTTAAGTGATAAGAGAATTAGACTTACGTATGGAGAATTTTCGAGTCGTTTGTGTTTGTATTGACGCTAACATGTATATATATACAACTAATTAGCGTTACTGTTGTGGTTTGTTTGTGAGGATATTTTATGTAGAATTCTTGGTTTGAATCCGGTCACCACATTTTCTGCTGTTCGGGTGTGTTTCTTTGAATATGGGTTGGTCAATAGACCAATAGCTAATAAAAAAAAAGAATTTAAAAGTACTTAGGAAAGCTTAAAGAGTCATTGCATAGGTACAATTAGCAATACGAATTGATAACTAGGTTTGAGTATGACATAAATGTGCATTGTAGTAAATATGTATTAATGTTTCAGGCCCAGGTTGTTCTTCAATTGGCTATGGAGAAGCAGAAGAACTTGGACCCTTTTTCCCCCAAAATAGCAGCCAACCTAAGCTCAAGTTGAACCCATATTCTTGGAATAAAGGTGGGTCAATTACAAATTGATAACTTTCACACTCGTAACATAATTTGAGATAAACTTGTATATCTTGCACAATTTTCTCATATATATACACACACACATTTTAAATATTGCAATAAAGAAGTTCTCACATAATATTAATAATTTTCTCATATAGTTATTCGATTTTTTTTATTTTTAAATAAACTAAATCTACTATAAAGAAAAATAATAATCTCATACCATATGATTTCTACAGCTGCAAATCTTTTGTTTCTTGAATCCCCTGTTGGAGTGGGTTTTTCCTACACCAACACCACCAGTGATATACGTGAACTTGGTGACACCATTACAGGTAACAACAATTCTATATTGTCTAAGGATTGATACAATTTTTATTAGTACTTTCACTTTAGATGATTTATTGATGAATACTTTATTTCATTGACCAGCTAAGGATTCACACACATTCCTCATCAATTGGTTTAAAAGATTTCCTCAATTCAAATCACATGATTTCTACATTGCTGGTGAAAGCTACGCAGGTATATTTAACTTCTAAAATAATTTTAATAAACACTAAGTTCCTAACTTTAAATTAATTTGAGTTTGTATTGAAATTTCAGGACATTATGTTCCACAACTTTCTGAGCTCATTTTGGATAACAGTCACAATTCTTCCCAAGAAGATTACATTAACTTCAAAGGGATCATGGTATAATCAAATTAATCAATAATAATAAAAGTCATAAAGAAGTTAACTAAGTTATAAACCAATATTAATATTAATTAGACACAATGAAATGTTTGGTACAGATTGGAAATGTTTTATTGGATGATGAAACAGATCAAAAAGGTATGATAGAGTATGCATGGGACCATGCTGTAATATCTGATGGTTTATACCATAACATCACAACCATATGCAACTTCAGCCATCCAATTCAAAATCAAACAGATGAATGCAATACAGAACTTAATAAATACTTTGATGTGTATAAGATTATAGACATGTACAGCTTGTACGCTCCCATGTGTTTCAGCAACATCAGCAATGTCAGAAGGGAAGCCCCTCATTCTTTTTCCAAATTTGTGAGTTTCATCAATCTTATACCTTCCAATTTAGAACTTAAAAACTAGTATTTGTAACTTTGTAAATTGCATAAAAGCACAATAGTGCACTACCAAATAAGGTACATTTGCAACTAACGTTACTTGCCTAGTGAAATGCACAACTAGTATGATATGCAATATTTTATAGATACTAGCATATGAGACAATATTTTATAGATACTAGCATATGAGACATGTGCTCAATATAAGTGTGAGAAAATCAATTGAAAAACGCAGATAAATGAACCATGTTTTATTAATAGTATTTTTTTCCGTATATAATAACTAATAGACGCTGACATGTGACGCAACGTGGATAAGCGAACTATGTTTTATTAATGAGAAATGATATTTGTACAACCAATTTGATACAACTTTTGTACAACTTTCTCTCTCATACTCACATTATGTTTTTATTCTCTCTCTTCCTTTTTCTCTCTCTATTGTTTTTGACCAATGAAAAGAGAGAACAACAAAGTTGTCTTAAAGGTTGTAACAAAATGGTTGTCAAAATATCACTACTCTTTATTAATAGTATGATACATATGATGTTGAAATTGTCAAATTTAACAGGACGGGTGGCATAAAAATCTAGCAGGATATGATCCATGTGCATCAGATTATACTGCAGCATACCTAAATAAGCCAGAGGTTCAAAAGGCACTTCATGCTAATGTCACAAAAATTCCCTATCCATGGACTCATTGCAGGTATGTGATTCACTAATTTCAATCAATTTTTGCGAACATATTACATATTAGAAAGTATACATATATAATCAAATCTAACTTATAAAGTGTCATTCATATATTAATACAAATCAACATTGATGCTGTCTTATGTTTTTGTCCTTAGTAATAATATAACATTTTGGAATGATGCACCTGTGTCCATGCTTCCTGTCCTCAAGAAGCTTATTGCTGGTGGTATTCGTATATGGGTTTACAGGTAAATAATTAATTGTAACATTTTTATTAATCCTTCGTAAAAAATAGACTAATCTAGTATATTTTTCTTAGAAGTTCTCTCACTTCATTTTTCATAAATATGTTTATAGTAATTTTTGGACATTCAGAACCATACGTTTGCTATATTTCTCTGTTTATTTTAAATAATTTTTTTATAAGTTGGAAGTTAATTCAAAAGTGATATTATTTATTTAATTTTTTTCTTTATTTATTCTTTTTCTAATTTACTTTATTTTCATTATTATAGTGGAGATACGGATGGAAGAATTCCAGTAACTGCAACAAGATACACATTAAGAAAATTGGGACTCCCTATAGTTCAAGATTGGACCCCTTGGTATACTAGTCGCCAGGTAAACCAAGTTGTTCTAAGTTGATTTATTTATCAAAAGTTCATATTATTCATTTTAATTACTTTTTAAAATAAATTGTTCACGATGTCTTATCAATTAAATTATGCTAGAGTATAGTAATATACAACTTCTACTTGAGGTTTTGTTCCCCTTAACTTCAACTTATATTCTTGTTTGTCTATTAAAAAAAACTTGTATTCTTGTTCTTGTGAATAAATAGAAATAGAACTAATAATGTTGGCTCAATGCGAAAGTGGTGAATGAACTTTGGATTACATGACTGTTTTCTCTAAGAATCAGAGGGTTAACAAAAAAAAAAGTGGTATGACGAAGTGACTTTTCTAAAACTGCATACTCATAAAATTTCCTCAAAATTGTAATTTTTGTTTTAACATGAATTCATGTGGCATTGATATTCTTATTATTCCATTTTTTTTTCTTCTTATTATTCCAATTTTACTCTCCACTTTATGAAACATTTTTTTATTTGTGGTATGAAATTATAGGTGGGAGGATGGAACATTGTATATGACGGACTAACATTTGTGACCATAAGAGGTGCTGGTCATCAAGTTCCTACTTTTGCTCCCAAACAAGCTCTTCAGCTAGTTCGACATTTCTTAGTCAATAAGAAATTACCACAACATCCTATTTAA
Protein sequence

>MS.gene003427.t1

MMLASPGCSSIGYGEAEELGPFFPQNSSQPKLKLNPYSWNKAANLLFLESPVGVGFSYTNTTSDIRELGDTITAKDSHTFLINWFKRFPQFKSHDFYIAGESYAGHYVPQLSELILDNSHNSSQEDYINFKGIMIGNVLLDDETDQKGMIEYAWDHAVISDGLYHNITTICNFSHPIQNQTDECNTELNKYFDVYKIIDMYSLYAPMCFSNISNVRREAPHSFSKFDGWHKNLAGYDPCASDYTAAYLNKPEVQKALHANVTKIPYPWTHCSNNITFWNDAPVSMLPVLKKLIAGGIRIWVYSGDTDGRIPVTATRYTLRKLGLPIVQDWTPWYTSRQVGGWNIVYDGLTFVTIRGAGHQVPTFAPKQALQLVRHFLVNKKLPQHPI