AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene049175

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049175.t1 MTR_3g098470 99.678 311 1 0 1 311 1 311 0.0 642
MS.gene049175.t1 MTR_8g096060 80.511 313 56 2 1 311 1 310 0.0 521
MS.gene049175.t1 MTR_1g031180 77.926 299 66 0 13 311 18 316 2.53e-180 500
MS.gene049175.t1 MTR_4g087460 41.695 295 154 8 29 311 16 304 3.61e-73 228
MS.gene049175.t1 MTR_8g032010 41.077 297 138 5 33 303 5 290 2.69e-69 218
MS.gene049175.t1 MTR_8g032000 41.017 295 137 5 37 305 9 292 8.94e-69 216
MS.gene049175.t1 MTR_8g031960 40.667 300 140 6 36 309 9 296 1.31e-67 213
MS.gene049175.t1 MTR_7g076660 41.522 289 144 6 37 311 9 286 1.33e-67 213
MS.gene049175.t1 MTR_7g076660 41.522 289 144 6 37 311 10 287 1.61e-67 213
MS.gene049175.t1 MTR_8g031980 39.604 303 145 6 34 310 6 296 2.97e-67 212
MS.gene049175.t1 MTR_8g031990 39.344 305 148 6 33 311 5 298 7.27e-67 211
MS.gene049175.t1 MTR_7g076655 41.522 289 144 6 37 311 9 286 6.38e-66 208
MS.gene049175.t1 MTR_8g032020 40.397 302 142 6 37 311 10 300 7.79e-66 209
MS.gene049175.t1 MTR_5g062280 40.132 304 146 6 32 311 4 295 1.95e-64 205
MS.gene049175.t1 MTR_8g031910 41.724 290 143 6 32 306 4 282 1.04e-62 200
MS.gene049175.t1 MTR_3g079320 38.079 302 148 5 31 303 7 298 1.13e-61 198
MS.gene049175.t1 MTR_8g031940 39.456 294 140 6 37 303 9 291 4.59e-61 196
MS.gene049175.t1 MTR_8g031630 38.538 301 147 9 37 311 9 297 5.44e-61 196
MS.gene049175.t1 MTR_8g031900 37.162 296 154 5 37 311 9 293 6.69e-61 196
MS.gene049175.t1 MTR_8g031610 38.667 300 147 8 37 311 7 294 8.82e-61 196
MS.gene049175.t1 MTR_8g031650 38.333 300 147 9 38 311 104 391 4.69e-59 194
MS.gene049175.t1 MTR_8g031650 39.669 121 62 4 177 292 2 116 5.73e-16 78.2
MS.gene049175.t1 MTR_7g076660 40.074 272 138 6 54 311 2 262 7.27e-59 189
MS.gene049175.t1 MTR_8g031600 38.614 303 149 7 35 311 7 298 5.38e-58 189
MS.gene049175.t1 MTR_8g031650 38.333 300 147 9 38 311 270 557 1.58e-57 194
MS.gene049175.t1 MTR_8g031650 39.298 285 133 9 37 292 9 282 6.31e-55 187
MS.gene049175.t1 MTR_7g076665 37.919 298 151 5 37 311 9 295 2.42e-56 184
MS.gene049175.t1 MTR_7g106800 37.234 282 128 7 37 311 11 250 2.11e-49 166
MS.gene049175.t1 MTR_8g031850 33.453 278 151 4 37 311 9 255 9.37e-47 158
MS.gene049175.t1 MTR_5g083430 37.245 196 111 4 116 311 1 184 3.29e-37 131
MS.gene049175.t1 MTR_8g032030 42.012 169 70 3 34 175 6 173 4.53e-35 127
MS.gene049175.t1 MTR_4g105310 29.252 294 150 11 37 308 51 308 1.71e-16 79.3
MS.gene049175.t1 MTR_8g099995 27.907 301 147 14 37 308 38 297 2.62e-14 72.8
MS.gene049175.t1 MTR_8g098415 28.669 293 146 13 37 305 32 285 2.13e-13 70.1
MS.gene049175.t1 MTR_8g098415 28.669 293 146 13 37 305 32 285 2.73e-13 69.7
MS.gene049175.t1 MTR_4g105310 29.457 258 133 9 37 287 51 266 5.36e-13 68.2
MS.gene049175.t1 MTR_3g068070 34.028 144 92 3 29 170 12 154 1.36e-12 67.0
MS.gene049175.t1 MTR_1g100305 28.425 292 148 13 37 305 32 285 1.65e-11 64.3
MS.gene049175.t1 MTR_8g442750 29.932 147 96 2 33 172 15 161 5.59e-11 62.8
MS.gene049175.t1 MTR_7g071680 31.034 174 110 4 10 177 6 175 6.53e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049175.t1 AT5G51030 69.968 313 92 1 1 311 2 314 4.85e-173 482
MS.gene049175.t1 AT5G61830 67.089 316 99 3 1 311 1 316 8.45e-158 443
MS.gene049175.t1 AT5G61830 67.089 316 99 3 1 311 1 316 8.45e-158 443
MS.gene049175.t1 AT3G59710 42.756 283 151 5 23 303 7 280 2.60e-77 238
MS.gene049175.t1 AT3G59710 41.554 296 161 6 11 303 23 309 4.75e-76 236
MS.gene049175.t1 AT3G59710 42.199 282 152 5 24 303 18 290 1.14e-74 231
MS.gene049175.t1 AT3G61220 41.414 297 140 6 37 310 9 294 9.61e-67 211
MS.gene049175.t1 AT3G61220 41.414 297 140 6 37 310 16 301 1.41e-66 211
MS.gene049175.t1 AT3G61220 41.414 297 140 6 37 310 40 325 3.11e-66 211
MS.gene049175.t1 AT2G24190 40.741 297 142 6 37 310 9 294 1.94e-65 207
MS.gene049175.t1 AT2G24190 40.741 297 142 6 37 310 14 299 2.50e-65 207
MS.gene049175.t1 AT1G01800 39.535 301 146 7 36 311 6 295 1.56e-64 205
MS.gene049175.t1 AT1G01800 39.891 183 99 3 129 311 89 260 2.07e-37 134
MS.gene049175.t1 AT4G23420 30.375 293 142 11 37 305 49 303 3.92e-16 78.2
MS.gene049175.t1 AT4G23420 30.375 293 142 11 37 305 32 286 8.24e-16 77.0
MS.gene049175.t1 AT4G23420 30.375 293 142 11 37 305 32 286 8.24e-16 77.0
MS.gene049175.t1 AT5G50130 30.180 222 126 7 25 221 27 244 1.87e-15 75.1
MS.gene049175.t1 AT5G50130 26.984 315 159 11 25 310 27 299 1.92e-15 76.3
MS.gene049175.t1 AT5G50130 30.317 221 126 7 25 221 27 243 2.83e-15 74.7
MS.gene049175.t1 AT2G37540 27.211 294 149 11 37 305 36 289 2.98e-15 75.5
MS.gene049175.t1 AT5G50130 30.180 222 126 7 25 221 27 244 3.11e-15 75.5
MS.gene049175.t1 AT5G50130 30.317 221 126 7 25 221 27 243 3.85e-15 75.1
MS.gene049175.t1 AT5G50130 27.575 301 147 11 39 310 8 266 1.61e-13 70.5
MS.gene049175.t1 AT5G50130 27.667 300 147 11 39 310 8 265 2.14e-13 70.1
MS.gene049175.t1 AT5G50130 31.250 208 114 7 39 221 8 211 2.91e-13 69.3
MS.gene049175.t1 AT5G50130 31.401 207 114 7 39 221 8 210 3.69e-13 68.9
MS.gene049175.t1 AT4G23420 31.064 235 133 10 37 248 32 260 1.72e-12 67.0
MS.gene049175.t1 AT4G23420 31.064 235 131 9 37 248 32 258 6.22e-12 65.1
MS.gene049175.t1 AT1G64590 27.703 296 154 12 37 308 37 296 1.98e-11 64.3
MS.gene049175.t1 AT4G20760 28.834 163 100 4 33 179 42 204 6.66e-11 62.4
MS.gene049175.t1 AT4G20760 28.834 163 100 4 33 179 62 224 6.99e-11 62.8
MS.gene049175.t1 AT4G23430 27.682 289 145 11 37 305 32 276 9.26e-11 62.4

Find 72 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTATTGAAACAAATTATTA+TGG 0.185787 3.3:+81000277 MS.gene049175:CDS
TCACGTTTGGGTCGTCTAAA+TGG 0.278823 3.3:+81000376 MS.gene049175:CDS
GAAACTATTGCCGTGGTTAC+TGG 0.305445 3.3:+80999845 MS.gene049175:CDS
GAAGATGGAAGTTGGAAAAC+AGG 0.313685 3.3:+81001118 MS.gene049175:CDS
TGTTAATGTGAGTTCACGTT+TGG 0.336548 3.3:+81000363 MS.gene049175:CDS
ATATGGTGGTCTAGATATTC+TGG 0.337491 3.3:+81000069 MS.gene049175:CDS
GTTCCTGATCAAGAGATTAC+AGG 0.348866 3.3:+81001355 MS.gene049175:CDS
AACCTCTGGTGATCAGAATA+AGG 0.350644 3.3:-80999506 None:intergenic
GTTGAACAAGGGGCTGATAC+TGG 0.370186 3.3:+81001316 MS.gene049175:CDS
ACTGGTGGAAACCGAGGAAT+TGG 0.387985 3.3:+80999863 MS.gene049175:CDS
AAGGGGCTGATACTGGAGTT+TGG 0.401013 3.3:+81001323 MS.gene049175:CDS
TAATACTTACACAAGGTATA+TGG 0.404526 3.3:+81001189 MS.gene049175:CDS
AATAAGGAATGGTTCTCAAA+AGG 0.409576 3.3:-80999490 None:intergenic
GTATTGAAACAAATTATTAT+GGG 0.416441 3.3:+81000278 MS.gene049175:CDS
TACATTAATTGTTACTGTCC+TGG 0.416925 3.3:+81001250 MS.gene049175:CDS
AATAGAAAATGAGGAGTTGA+GGG 0.424375 3.3:+81001021 MS.gene049175:CDS
TGGGTGAAAACTGCTCTAAC+AGG 0.426724 3.3:+81001274 MS.gene049175:CDS
GGTCTTGCAAGAAGGTGGTT+TGG 0.437244 3.3:+80999973 MS.gene049175:CDS
AACCACGGCAATAGTTTCCT+TGG 0.438839 3.3:-80999840 None:intergenic
CATAGCTTCAATCATTCTCT+TGG 0.438910 3.3:-81000302 None:intergenic
TCAAGACAACTTGCCGATCA+TGG 0.440043 3.3:+80999896 MS.gene049175:CDS
TTTCTACAACAAGTAGAAGA+TGG 0.445780 3.3:+81001103 MS.gene049175:CDS
GTTAATGTGAGTTCACGTTT+GGG 0.446917 3.3:+81000364 MS.gene049175:CDS
TATTTATAGGTAAATAATGC+TGG 0.447071 3.3:+81000205 MS.gene049175:intron
TTCCCTGTAATCTCTTGATC+AGG 0.452132 3.3:-81001358 None:intergenic
ATAAGGAATGGTTCTCAAAA+GGG 0.455119 3.3:-80999489 None:intergenic
TTGATGAAGCCATCTGCTGC+TGG 0.468286 3.3:+81000331 MS.gene049175:CDS
GAATAGAAAATGAGGAGTTG+AGG 0.479987 3.3:+81001020 MS.gene049175:CDS
GAGTGGTTGAAAGAAGAATA+TGG 0.483507 3.3:+81000052 MS.gene049175:CDS
TGGCAAAGAAGCTCTCTGAT+AGG 0.485202 3.3:+81001209 MS.gene049175:CDS
AACAAGTAGAAGATGGAAGT+TGG 0.488568 3.3:+81001110 MS.gene049175:CDS
AAATCTCAAAACCAATTCCT+CGG 0.493628 3.3:-80999874 None:intergenic
ACTGCTCTAACAGGTTATGC+AGG 0.511056 3.3:+81001283 MS.gene049175:CDS
GAATAGTCAGTAAATGTTTG+AGG 0.522157 3.3:-81001148 None:intergenic
TATATAAAGGTGGTGGTCCA+AGG 0.527053 3.3:+80999823 MS.gene049175:intron
TAATTGTTACTGTCCTGGTT+GGG 0.532693 3.3:+81001255 MS.gene049175:CDS
TTAATTGTTACTGTCCTGGT+TGG 0.540425 3.3:+81001254 MS.gene049175:CDS
GTTACAGAGAATAGAAAATG+AGG 0.541317 3.3:+81001012 MS.gene049175:intron
TAATCTCTTGATCAGGAACA+AGG 0.543992 3.3:-81001351 None:intergenic
GCAGCAGATGGCTTCATCAA+AGG 0.554615 3.3:-81000328 None:intergenic
CACAACAGTAACTCCATGAT+CGG 0.568398 3.3:-80999909 None:intergenic
ATCAAGGTCTTGCAAGAAGG+TGG 0.574693 3.3:+80999968 MS.gene049175:CDS
TCAATCAAGGTCTTGCAAGA+AGG 0.574992 3.3:+80999965 MS.gene049175:CDS
TGCTGGTGTTAATTCCAACA+TGG 0.582462 3.3:+81000222 MS.gene049175:CDS
TTCCTGATCAAGAGATTACA+GGG 0.585333 3.3:+81001356 MS.gene049175:CDS
TTCCTTATTCTGATCACCAG+AGG 0.589818 3.3:+80999504 MS.gene049175:CDS
TGTCGGTGTTGAATCAATCA+AGG 0.590040 3.3:+80999952 MS.gene049175:CDS
GGAAGTGTTACAGTTGAACA+AGG 0.606460 3.3:+81001304 MS.gene049175:CDS
GATGGAAGTTGGAAAACAGG+AGG 0.608453 3.3:+81001121 MS.gene049175:CDS
ATGGAAGTTGGAAAACAGGA+GGG 0.612855 3.3:+81001122 MS.gene049175:CDS
GCTGGTGTTAATTCCAACAT+GGG 0.616735 3.3:+81000223 MS.gene049175:CDS
ACTATTGCCGTGGTTACTGG+TGG 0.620364 3.3:+80999848 MS.gene049175:CDS
ATGAAGCCATCTGCTGCTGG+TGG 0.625719 3.3:+81000334 MS.gene049175:CDS
ATATATCAAGTTGATGACAA+TGG 0.627304 3.3:-81000000 None:intergenic
ATACGACCACCAGCAGCAGA+TGG 0.629788 3.3:-81000340 None:intergenic
CTGGTGATCAGAATAAGGAA+TGG 0.631585 3.3:-80999501 None:intergenic
AAGTGTTACAGTTGAACAAG+GGG 0.633975 3.3:+81001306 MS.gene049175:CDS
GTCCAAGGAAACTATTGCCG+TGG 0.637747 3.3:+80999838 MS.gene049175:CDS
TGATGTGGAATCTCTTTCAG+AGG 0.642229 3.3:+81001054 MS.gene049175:CDS
GAGGGAGAAACTGAGTGATG+TGG 0.647729 3.3:+81001039 MS.gene049175:CDS
AAGCTCTCTGATAGGCCAGA+GGG 0.648245 3.3:+81001217 MS.gene049175:CDS
TCGGTTTCCACCAGTAACCA+CGG 0.650998 3.3:-80999855 None:intergenic
TGAGTTATCAGATCCCATGT+TGG 0.651992 3.3:-81000236 None:intergenic
ACATCAAGAGATGCTAGTGT+CGG 0.652928 3.3:+80999935 MS.gene049175:CDS
GTGGTTACTGGTGGAAACCG+AGG 0.666460 3.3:+80999857 MS.gene049175:CDS
GAAGTGTTACAGTTGAACAA+GGG 0.666525 3.3:+81001305 MS.gene049175:CDS
GAAGCTCTCTGATAGGCCAG+AGG 0.680052 3.3:+81001216 MS.gene049175:CDS
GAAGTTGGAAAACAGGAGGG+TGG 0.681062 3.3:+81001125 MS.gene049175:CDS
AATCTCTTGATCAGGAACAA+GGG 0.687916 3.3:-81001350 None:intergenic
CTGTCAATGAATTTGCTGAG+TGG 0.698650 3.3:+81000035 MS.gene049175:CDS
TCGCAGTTAATACTTACACA+AGG 0.727216 3.3:+81001182 MS.gene049175:CDS
TGGTTGAAAGAAGAATATGG+TGG 0.790075 3.3:+81000055 MS.gene049175:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTAAAATCATGATTTTAA+CGG - chr3.3:80999792-80999811 None:intergenic 10.0%
!! ATTGTTAAATACTATGATAT+GGG - chr3.3:81000820-81000839 None:intergenic 15.0%
!! GTATTGAAACAAATTATTAT+GGG + chr3.3:81000278-81000297 MS.gene049175:CDS 15.0%
!! TGTATTGAAACAAATTATTA+TGG + chr3.3:81000277-81000296 MS.gene049175:CDS 15.0%
!! TTGAACAATAAAAGAATTAT+AGG - chr3.3:80999554-80999573 None:intergenic 15.0%
!!! AATGACATTTTATAGTTTTA+GGG + chr3.3:81000865-81000884 MS.gene049175:intron 15.0%
!!! GCTATAATTTTGTTATTTAT+AGG + chr3.3:81000192-81000211 MS.gene049175:intron 15.0%
!!! TATAATTATCAATTGTTTCT+TGG + chr3.3:81000170-81000189 MS.gene049175:intron 15.0%
!! CATATCATAGTATTTAACAA+TGG + chr3.3:81000819-81000838 MS.gene049175:intron 20.0%
!! CATTGTTAAATACTATGATA+TGG - chr3.3:81000821-81000840 None:intergenic 20.0%
!! TATGTTACCTTTCTATATAA+AGG + chr3.3:80999810-80999829 MS.gene049175:intron 20.0%
!! TATTTATAGGTAAATAATGC+TGG + chr3.3:81000205-81000224 MS.gene049175:intron 20.0%
!!! GAATGACATTTTATAGTTTT+AGG + chr3.3:81000864-81000883 MS.gene049175:intron 20.0%
!!! TTAATTTGACTAAGAAACTT+AGG - chr3.3:81000729-81000748 None:intergenic 20.0%
!!! TTCGACTTAATTTTGTTTAT+AGG + chr3.3:81000129-81000148 MS.gene049175:intron 20.0%
! ATATATTTCCCAATTAGCAA+GGG + chr3.3:81000888-81000907 MS.gene049175:intron 25.0%
! ATGAGATACCTAAATTTCAT+AGG - chr3.3:81000640-81000659 None:intergenic 25.0%
! ATGTTCATATGATAACAAGA+AGG - chr3.3:80999602-80999621 None:intergenic 25.0%
! CATCATTGAAATTGTATGTT+TGG - chr3.3:81000923-81000942 None:intergenic 25.0%
! GACTGGATTGAAATATATAT+GGG - chr3.3:80999692-80999711 None:intergenic 25.0%
! TAATACTTACACAAGGTATA+TGG + chr3.3:81001189-81001208 MS.gene049175:CDS 25.0%
! TAATGAGCTTATATTGATGT+AGG + chr3.3:81000753-81000772 MS.gene049175:intron 25.0%
! TACATCAATATAAGCTCATT+AGG - chr3.3:81000754-81000773 None:intergenic 25.0%
! TATAAGATCAAATTAACCTC+TGG - chr3.3:80999523-80999542 None:intergenic 25.0%
! TATGAAATTTAGGTATCTCA+TGG + chr3.3:81000639-81000658 MS.gene049175:intron 25.0%
! TTAATCTGCCTATGAAATTT+AGG + chr3.3:81000629-81000648 MS.gene049175:intron 25.0%
!!! AAGTCGAATTTCACATTTTT+TGG - chr3.3:81000117-81000136 None:intergenic 25.0%
!!! ATATATCAAGTTGATGACAA+TGG - chr3.3:81000003-81000022 None:intergenic 25.0%
AAATCTCAAAACCAATTCCT+CGG - chr3.3:80999877-80999896 None:intergenic 30.0%
AATAAGGAATGGTTCTCAAA+AGG - chr3.3:80999493-80999512 None:intergenic 30.0%
AATAGAAAATGAGGAGTTGA+GGG + chr3.3:81001021-81001040 MS.gene049175:CDS 30.0%
ATAAGGAATGGTTCTCAAAA+GGG - chr3.3:80999492-80999511 None:intergenic 30.0%
GAATAGTCAGTAAATGTTTG+AGG - chr3.3:81001151-81001170 None:intergenic 30.0%
GATATATTTCCCAATTAGCA+AGG + chr3.3:81000887-81000906 MS.gene049175:intron 30.0%
GGACTGGATTGAAATATATA+TGG - chr3.3:80999693-80999712 None:intergenic 30.0%
GTTACAGAGAATAGAAAATG+AGG + chr3.3:81001012-81001031 MS.gene049175:intron 30.0%
GTTACCTTTCTATATAAAGG+TGG + chr3.3:80999813-80999832 MS.gene049175:intron 30.0%
TACATTAATTGTTACTGTCC+TGG + chr3.3:81001250-81001269 MS.gene049175:CDS 30.0%
TGTCATTCAAAATGATTCCT+CGG - chr3.3:81000852-81000871 None:intergenic 30.0%
TTCATAGGCAGATTAATCTA+CGG - chr3.3:81000625-81000644 None:intergenic 30.0%
TTTCTACAACAAGTAGAAGA+TGG + chr3.3:81001103-81001122 MS.gene049175:CDS 30.0%
AACAAGTAGAAGATGGAAGT+TGG + chr3.3:81001110-81001129 MS.gene049175:CDS 35.0%
AAGTGTTACAGTTGAACAAG+GGG + chr3.3:81001306-81001325 MS.gene049175:CDS 35.0%
AATACTATGATATGGGCATG+TGG - chr3.3:81000813-81000832 None:intergenic 35.0%
AATCTCTTGATCAGGAACAA+GGG - chr3.3:81001353-81001372 None:intergenic 35.0%
ACCACCACCTTTATATAGAA+AGG - chr3.3:80999820-80999839 None:intergenic 35.0%
ACCTTTCTATATAAAGGTGG+TGG + chr3.3:80999816-80999835 MS.gene049175:intron 35.0%
ATATGGTGGTCTAGATATTC+TGG + chr3.3:81000069-81000088 MS.gene049175:CDS 35.0%
CAAATAGTCCCTTGCTAATT+GGG - chr3.3:81000899-81000918 None:intergenic 35.0%
CATAGCTTCAATCATTCTCT+TGG - chr3.3:81000305-81000324 None:intergenic 35.0%
GAATAGAAAATGAGGAGTTG+AGG + chr3.3:81001020-81001039 MS.gene049175:CDS 35.0%
GAGTGGTTGAAAGAAGAATA+TGG + chr3.3:81000052-81000071 MS.gene049175:CDS 35.0%
GTTAATGTGAGTTCACGTTT+GGG + chr3.3:81000364-81000383 MS.gene049175:CDS 35.0%
TAATCTCTTGATCAGGAACA+AGG - chr3.3:81001354-81001373 None:intergenic 35.0%
TAATTGTTACTGTCCTGGTT+GGG + chr3.3:81001255-81001274 MS.gene049175:CDS 35.0%
TCAAATAGTCCCTTGCTAAT+TGG - chr3.3:81000900-81000919 None:intergenic 35.0%
TCACTAGTTGCAATTGTTCA+TGG - chr3.3:81000686-81000705 None:intergenic 35.0%
TCGCAGTTAATACTTACACA+AGG + chr3.3:81001182-81001201 MS.gene049175:CDS 35.0%
TGGTTGAAAGAAGAATATGG+TGG + chr3.3:81000055-81000074 MS.gene049175:CDS 35.0%
TGTTAATGTGAGTTCACGTT+TGG + chr3.3:81000363-81000382 MS.gene049175:CDS 35.0%
TTAACAATGGAAAGTAACCG+AGG + chr3.3:81000832-81000851 MS.gene049175:intron 35.0%
TTAATTGTTACTGTCCTGGT+TGG + chr3.3:81001254-81001273 MS.gene049175:CDS 35.0%
TTCCTGATCAAGAGATTACA+GGG + chr3.3:81001356-81001375 MS.gene049175:CDS 35.0%
! ATGTAAATCTTTTCACCCTC+TGG - chr3.3:81001235-81001254 None:intergenic 35.0%
! GAAGTGTTACAGTTGAACAA+GGG + chr3.3:81001305-81001324 MS.gene049175:CDS 35.0%
! TAGGTATCTCATGGACTAAT+TGG + chr3.3:81000648-81000667 MS.gene049175:intron 35.0%
AACCTCTGGTGATCAGAATA+AGG - chr3.3:80999509-80999528 None:intergenic 40.0%
ACATCAAGAGATGCTAGTGT+CGG + chr3.3:80999935-80999954 MS.gene049175:CDS 40.0%
ATCCAATGACCACCAATGTA+CGG + chr3.3:80999724-80999743 MS.gene049175:intron 40.0%
ATGGAAGTTGGAAAACAGGA+GGG + chr3.3:81001122-81001141 MS.gene049175:CDS 40.0%
CACAACAGTAACTCCATGAT+CGG - chr3.3:80999912-80999931 None:intergenic 40.0%
CATTCACACAATCCGTACAT+TGG - chr3.3:80999739-80999758 None:intergenic 40.0%
CATTCACCCAATCAACATGT+TGG + chr3.3:80999641-80999660 MS.gene049175:intron 40.0%
CTCCAACGCTAAAAGGTTAA+TGG - chr3.3:81000563-81000582 None:intergenic 40.0%
CTGGTGATCAGAATAAGGAA+TGG - chr3.3:80999504-80999523 None:intergenic 40.0%
GAAGATGGAAGTTGGAAAAC+AGG + chr3.3:81001118-81001137 MS.gene049175:CDS 40.0%
GTTCCTGATCAAGAGATTAC+AGG + chr3.3:81001355-81001374 MS.gene049175:CDS 40.0%
TCAATCAAGGTCTTGCAAGA+AGG + chr3.3:80999965-80999984 MS.gene049175:CDS 40.0%
TCATAGTCTCCAACGCTAAA+AGG - chr3.3:81000570-81000589 None:intergenic 40.0%
TGAGTTATCAGATCCCATGT+TGG - chr3.3:81000239-81000258 None:intergenic 40.0%
TGATGTGGAATCTCTTTCAG+AGG + chr3.3:81001054-81001073 MS.gene049175:CDS 40.0%
TTCCCTGTAATCTCTTGATC+AGG - chr3.3:81001361-81001380 None:intergenic 40.0%
TTCCTTATTCTGATCACCAG+AGG + chr3.3:80999504-80999523 MS.gene049175:CDS 40.0%
! CTCCATTAACCTTTTAGCGT+TGG + chr3.3:81000558-81000577 MS.gene049175:intron 40.0%
! GCTGGTGTTAATTCCAACAT+GGG + chr3.3:81000223-81000242 MS.gene049175:CDS 40.0%
! TATATAAAGGTGGTGGTCCA+AGG + chr3.3:80999823-80999842 MS.gene049175:intron 40.0%
! TGCTGGTGTTAATTCCAACA+TGG + chr3.3:81000222-81000241 MS.gene049175:CDS 40.0%
!! CTGTCAATGAATTTGCTGAG+TGG + chr3.3:81000035-81000054 MS.gene049175:CDS 40.0%
!! GGAAGTGTTACAGTTGAACA+AGG + chr3.3:81001304-81001323 MS.gene049175:CDS 40.0%
!! TGTCGGTGTTGAATCAATCA+AGG + chr3.3:80999952-80999971 MS.gene049175:CDS 40.0%
AACCACGGCAATAGTTTCCT+TGG - chr3.3:80999843-80999862 None:intergenic 45.0%
ACGACCACCAACATGTTGAT+TGG - chr3.3:80999651-80999670 None:intergenic 45.0%
ACTGCTCTAACAGGTTATGC+AGG + chr3.3:81001283-81001302 MS.gene049175:CDS 45.0%
AGGCAGATTAATCTACGGTG+TGG - chr3.3:81000620-81000639 None:intergenic 45.0%
ATCAAGGTCTTGCAAGAAGG+TGG + chr3.3:80999968-80999987 MS.gene049175:CDS 45.0%
GAAACTATTGCCGTGGTTAC+TGG + chr3.3:80999845-80999864 MS.gene049175:CDS 45.0%
GATGGAAGTTGGAAAACAGG+AGG + chr3.3:81001121-81001140 MS.gene049175:CDS 45.0%
GTCATTGGATTGAGATCAGG+CGG - chr3.3:80999714-80999733 None:intergenic 45.0%
GTGGTCATTGGATTGAGATC+AGG - chr3.3:80999717-80999736 None:intergenic 45.0%
TCAAGACAACTTGCCGATCA+TGG + chr3.3:80999896-80999915 MS.gene049175:CDS 45.0%
TCACACAATCCGTACATTGG+TGG - chr3.3:80999736-80999755 None:intergenic 45.0%
TCACCCAATCAACATGTTGG+TGG + chr3.3:80999644-80999663 MS.gene049175:intron 45.0%
TCACGTTTGGGTCGTCTAAA+TGG + chr3.3:81000376-81000395 MS.gene049175:CDS 45.0%
TGGCAAAGAAGCTCTCTGAT+AGG + chr3.3:81001209-81001228 MS.gene049175:CDS 45.0%
TGGGTGAAAACTGCTCTAAC+AGG + chr3.3:81001274-81001293 MS.gene049175:CDS 45.0%
! ATCCGTACATTGGTGGTCAT+TGG - chr3.3:80999729-80999748 None:intergenic 45.0%
! CGACCACCAACATGTTGATT+GGG - chr3.3:80999650-80999669 None:intergenic 45.0%
! GTACGGATTGTGTGAATGTG+TGG + chr3.3:80999741-80999760 MS.gene049175:intron 45.0%
!! ACGGTGTGGTGCTATTAATG+TGG - chr3.3:81000606-81000625 None:intergenic 45.0%
!! ATCAACATGTTGGTGGTCGT+CGG + chr3.3:80999651-80999670 MS.gene049175:intron 45.0%
AAGCTCTCTGATAGGCCAGA+GGG + chr3.3:81001217-81001236 MS.gene049175:CDS 50.0%
AAGGGGCTGATACTGGAGTT+TGG + chr3.3:81001323-81001342 MS.gene049175:CDS 50.0%
ACTATTGCCGTGGTTACTGG+TGG + chr3.3:80999848-80999867 MS.gene049175:CDS 50.0%
ACTGGTGGAAACCGAGGAAT+TGG + chr3.3:80999863-80999882 MS.gene049175:CDS 50.0%
GAAGTTGGAAAACAGGAGGG+TGG + chr3.3:81001125-81001144 MS.gene049175:CDS 50.0%
GAGGGAGAAACTGAGTGATG+TGG + chr3.3:81001039-81001058 MS.gene049175:CDS 50.0%
GCAGCAGATGGCTTCATCAA+AGG - chr3.3:81000331-81000350 None:intergenic 50.0%
GGTCTTGCAAGAAGGTGGTT+TGG + chr3.3:80999973-80999992 MS.gene049175:CDS 50.0%
GTCCAAGGAAACTATTGCCG+TGG + chr3.3:80999838-80999857 MS.gene049175:CDS 50.0%
GTTGAACAAGGGGCTGATAC+TGG + chr3.3:81001316-81001335 MS.gene049175:CDS 50.0%
TCGGTTTCCACCAGTAACCA+CGG - chr3.3:80999858-80999877 None:intergenic 50.0%
TTGATGAAGCCATCTGCTGC+TGG + chr3.3:81000331-81000350 MS.gene049175:CDS 50.0%
! AGAGCAGTTTTCACCCAACC+AGG - chr3.3:81001271-81001290 None:intergenic 50.0%
ATACGACCACCAGCAGCAGA+TGG - chr3.3:81000343-81000362 None:intergenic 55.0%
ATGAAGCCATCTGCTGCTGG+TGG + chr3.3:81000334-81000353 MS.gene049175:CDS 55.0%
GAAGCTCTCTGATAGGCCAG+AGG + chr3.3:81001216-81001235 MS.gene049175:CDS 55.0%
GTGGTTACTGGTGGAAACCG+AGG + chr3.3:80999857-80999876 MS.gene049175:CDS 55.0%
TGGATTGAGATCAGGCGGAC+TGG - chr3.3:80999709-80999728 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 80999440 81001414 80999440 ID=MS.gene049175
chr3.3 mRNA 80999440 81001414 80999440 ID=MS.gene049175.t1;Parent=MS.gene049175
chr3.3 exon 80999440 80999525 80999440 ID=MS.gene049175.t1.exon1;Parent=MS.gene049175.t1
chr3.3 CDS 80999440 80999525 80999440 ID=cds.MS.gene049175.t1;Parent=MS.gene049175.t1
chr3.3 exon 80999832 81000090 80999832 ID=MS.gene049175.t1.exon2;Parent=MS.gene049175.t1
chr3.3 CDS 80999832 81000090 80999832 ID=cds.MS.gene049175.t1;Parent=MS.gene049175.t1
chr3.3 exon 81000214 81000408 81000214 ID=MS.gene049175.t1.exon3;Parent=MS.gene049175.t1
chr3.3 CDS 81000214 81000408 81000214 ID=cds.MS.gene049175.t1;Parent=MS.gene049175.t1
chr3.3 exon 81001019 81001414 81001019 ID=MS.gene049175.t1.exon4;Parent=MS.gene049175.t1
chr3.3 CDS 81001019 81001414 81001019 ID=cds.MS.gene049175.t1;Parent=MS.gene049175.t1
Gene Sequence

>MS.gene049175

ATGGGTCACAAAGAAAAATCACGAAAAGATAAGAGATTGCAAGAGATATCCCTTTTGAGAACCATTCCTTATTCTGATCACCAGAGGTTAATTTGATCTTATAGATATATACCTATAATTCTTTTATTGTTCAATAATATTAACTTTTAGTTAGTTTTTCCTTCTTGTTATCATATGAACATATGATTGTTAAATTCGATGCATTCACCCAATCAACATGTTGGTGGTCGTCGGATCAAGATTGAATAACCCATATATATTTCAATCCAGTCCGCCTGATCTCAATCCAATGACCACCAATGTACGGATTGTGTGAATGTGTGGATCGTTGATTGCAACAAATTAAAATCCGTTAAAATCATGATTTTAATATGTTACCTTTCTATATAAAGGTGGTGGTCCAAGGAAACTATTGCCGTGGTTACTGGTGGAAACCGAGGAATTGGTTTTGAGATTTCAAGACAACTTGCCGATCATGGAGTTACTGTTGTGTTGACATCAAGAGATGCTAGTGTCGGTGTTGAATCAATCAAGGTCTTGCAAGAAGGTGGTTTGGACGTCCATTGTCATCAACTTGATATATTAGATTCTTCATCTGTCAATGAATTTGCTGAGTGGTTGAAAGAAGAATATGGTGGTCTAGATATTCTGGTAAGTTTCTAAAATGTAATTAGCCAAAAAATGTGAAATTCGACTTAATTTTGTTTATAGGTGTTAGCGTTGTTAATTTTATAATTATCAATTGTTTCTTGGCTATAATTTTGTTATTTATAGGTAAATAATGCTGGTGTTAATTCCAACATGGGATCTGATAACTCAGTTGAAAATGCTCGTAAGTGTATTGAAACAAATTATTATGGGACCAAGAGAATGATTGAAGCTATGATTCCTTTGATGAAGCCATCTGCTGCTGGTGGTCGTATTGTTAATGTGAGTTCACGTTTGGGTCGTCTAAATGGCAAACGAAATGTAAGCAAACAATCTAATTGTTTCACTGAGTAATTTCTATTCTTGTAACTTGCAATACAGTTTTTGCTCTCAAAATTGTATGCATAATTGCATATAGTATGATTTTTGCAAATTTATAAATATAAATTTATATAAAATTAGAATAGTATCTCCATTAACCTTTTAGCGTTGGAGACTATGATATGACGCGTTAACCACATTAATAGCACCACACCGTAGATTAATCTGCCTATGAAATTTAGGTATCTCATGGACTAATTGGTTAGTAATTAGTCCATGAACAATTGCAACTAGTGACAAATTTGTTTTTAACATAACCTAAGTTTCTTAGTCAAATTAATTCCTAATGAGCTTATATTGATGTAGGATTAACGTGTAATGTGACATGTTCAATTAATATGCCACATGCCCATATCATAGTATTTAACAATGGAAAGTAACCGAGGAATCATTTTGAATGACATTTTATAGTTTTAGGGATATATTTCCCAATTAGCAAGGGACTATTTGACCAAACATACAATTTCAATGATGAATTTGTCTGTATACTCTTGATTCTCAAATTATGCGAAATGCAAAACTGATTAACTGTAACTTGATTGTGTTACAGAGAATAGAAAATGAGGAGTTGAGGGAGAAACTGAGTGATGTGGAATCTCTTTCAGAGGAACTAATTGATGAAACTATAAATAATTTTCTACAACAAGTAGAAGATGGAAGTTGGAAAACAGGAGGGTGGCCTCAAACATTTACTGACTATTCAGTATCAAAGCTCGCAGTTAATACTTACACAAGGTATATGGCAAAGAAGCTCTCTGATAGGCCAGAGGGTGAAAAGATTTACATTAATTGTTACTGTCCTGGTTGGGTGAAAACTGCTCTAACAGGTTATGCAGGAAGTGTTACAGTTGAACAAGGGGCTGATACTGGAGTTTGGATTGCCCTTGTTCCTGATCAAGAGATTACAGGGAAGTTTTTTGCTGAAAGAAGAGAGATTAACTTTTAA
Protein sequence

>MS.gene049175.t1

MGHKEKSRKDKRLQEISLLRTIPYSDHQRWWSKETIAVVTGGNRGIGFEISRQLADHGVTVVLTSRDASVGVESIKVLQEGGLDVHCHQLDILDSSSVNEFAEWLKEEYGGLDILVNNAGVNSNMGSDNSVENARKCIETNYYGTKRMIEAMIPLMKPSAAGGRIVNVSSRLGRLNGKRNRIENEELREKLSDVESLSEELIDETINNFLQQVEDGSWKTGGWPQTFTDYSVSKLAVNTYTRYMAKKLSDRPEGEKIYINCYCPGWVKTALTGYAGSVTVEQGADTGVWIALVPDQEITGKFFAERREINF