Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04987.t1 | XP_024633534.1 | 88 | 418 | 25 | 2 | 1 | 393 | 11 | 428 | 1.40E-134 | 489.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04987.t1 | B7FNB2 | 88.0 | 418 | 25 | 2 | 1 | 393 | 74 | 491 | 1.0e-134 | 489.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04987 | MS.gene04988 | 0.865305 | 6.50E-65 | -1.69E-46 |
| MS.gene04987 | MS.gene050277 | 0.807372 | 5.22E-50 | -1.69E-46 |
| MS.gene04987 | MS.gene052600 | 0.801885 | 7.29E-49 | -1.69E-46 |
| MS.gene04987 | MS.gene057079 | 0.803338 | 3.66E-49 | -1.69E-46 |
| MS.gene04987 | MS.gene057555 | 0.814275 | 1.67E-51 | -1.69E-46 |
| MS.gene04987 | MS.gene060297 | 0.816087 | 6.63E-52 | -1.69E-46 |
| MS.gene04987 | MS.gene068549 | 0.807115 | 5.92E-50 | -1.69E-46 |
| MS.gene04987 | MS.gene07368 | 0.824581 | 7.47E-54 | -1.69E-46 |
| MS.gene04987 | MS.gene073833 | 0.831911 | 1.28E-55 | -1.69E-46 |
| MS.gene04987 | MS.gene20912 | 0.815575 | 8.62E-52 | -1.69E-46 |
| MS.gene04987 | MS.gene22691 | 0.801544 | 8.57E-49 | -1.69E-46 |
| MS.gene04987 | MS.gene28218 | 0.811246 | 7.70E-51 | -1.69E-46 |
| MS.gene04987 | MS.gene30855 | 0.82031 | 7.34E-53 | -1.69E-46 |
| MS.gene04987 | MS.gene44540 | 0.812849 | 3.45E-51 | -1.69E-46 |
| MS.gene04987 | MS.gene44753 | 0.808625 | 2.83E-50 | -1.69E-46 |
| MS.gene04987 | MS.gene53383 | 0.809705 | 1.66E-50 | -1.69E-46 |
| MS.gene04987 | MS.gene61999 | 0.800093 | 1.69E-48 | -1.69E-46 |
| MS.gene04987 | MS.gene72128 | 0.820043 | 8.45E-53 | -1.69E-46 |
| MS.gene04987 | MS.gene72130 | 0.824858 | 6.43E-54 | -1.69E-46 |
| MS.gene04987 | MS.gene72894 | 0.804772 | 1.84E-49 | -1.69E-46 |
| MS.gene04987 | MS.gene76002 | 0.832633 | 8.46E-56 | -1.69E-46 |
| MS.gene04987 | MS.gene80668 | 0.801643 | 8.17E-49 | -1.69E-46 |
| MS.gene04987 | MS.gene80795 | 0.804063 | 2.59E-49 | -1.69E-46 |
| MS.gene04987 | MS.gene83734 | 0.802729 | 4.89E-49 | -1.69E-46 |
| MS.gene04987 | MS.gene91280 | 0.80188 | 7.31E-49 | -1.69E-46 |
| MS.gene04987 | MS.gene92849 | 0.828003 | 1.14E-54 | -1.69E-46 |
| MS.gene04987 | MS.gene93202 | 0.824243 | 8.97E-54 | -1.69E-46 |
| MS.gene04987 | MS.gene98277 | 0.812106 | 5.01E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04987.t1 | MTR_3g065220 | 91.148 | 418 | 12 | 2 | 1 | 393 | 74 | 491 | 0.0 | 751 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04987.t1 | AT5G37590 | 52.048 | 415 | 173 | 4 | 4 | 393 | 78 | 491 | 4.21e-142 | 416 |
| MS.gene04987.t1 | AT5G37590 | 51.562 | 384 | 162 | 2 | 4 | 363 | 78 | 461 | 2.40e-130 | 383 |
Find 76 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAGCAGAACCGTTGTATT+TGG | 0.238627 | 3.3:-58773464 | MS.gene04987:CDS |
| GTCGATTTATTCAGATAAAA+AGG | 0.240328 | 3.3:-58771967 | MS.gene04987:intron |
| GGAAGAATTGCAGGAGATTC+AGG | 0.248308 | 3.3:-58766296 | MS.gene04987:intron |
| CCAAGCAGCACTTGTCATAT+TGG | 0.295451 | 3.3:-58766483 | MS.gene04987:intron |
| AGCACTGGCGATGACTTAAA+TGG | 0.314212 | 3.3:-58774442 | MS.gene04987:CDS |
| TTGAGAATTCCATTATATTC+AGG | 0.319020 | 3.3:+58769020 | None:intergenic |
| AAAGAAGCTAAAGAAGGTTT+TGG | 0.327034 | 3.3:-58774165 | MS.gene04987:CDS |
| GGAAGCTATCAAAATCTTAG+AGG | 0.327155 | 3.3:-58773443 | MS.gene04987:CDS |
| ACTTCCTGGAGATCATATTC+AGG | 0.329597 | 3.3:-58768260 | MS.gene04987:intron |
| GGATGCCGAGATCATTATTA+AGG | 0.331789 | 3.3:-58770229 | MS.gene04987:CDS |
| ACACACCTCTAACATGCTTA+TGG | 0.335650 | 3.3:+58770203 | None:intergenic |
| AAAATCTTAGAGGATTCTTT+TGG | 0.337670 | 3.3:-58773433 | MS.gene04987:CDS |
| GAGAAAGATCCTGAATATAA+TGG | 0.352099 | 3.3:-58769029 | MS.gene04987:CDS |
| GTTCGGAGAGCCTTACTTCC+TGG | 0.359187 | 3.3:-58768274 | MS.gene04987:CDS |
| CAGAAGATGCTTGTAGGGTC+AGG | 0.379028 | 3.3:+58768425 | None:intergenic |
| TTATATTCAGGATCTTTCTC+TGG | 0.396404 | 3.3:+58769032 | None:intergenic |
| CAATATGACAAGTGCTGCTT+GGG | 0.399276 | 3.3:+58766484 | None:intergenic |
| TTGTTTCAGAAGATGCTTGT+AGG | 0.401174 | 3.3:+58768419 | None:intergenic |
| CGCGTCTTCATGCAACAATC+TGG | 0.412102 | 3.3:-58774124 | MS.gene04987:intron |
| GCAACGATCGAAACGTCTTC+CGG | 0.426044 | 3.3:-58774469 | MS.gene04987:CDS |
| GTTGGCTAAGGAAGAATTGC+AGG | 0.426289 | 3.3:-58766305 | MS.gene04987:CDS |
| CTTGTAGATGTCTCGAGGTT+CGG | 0.428878 | 3.3:-58768291 | MS.gene04987:intron |
| CCAATATGACAAGTGCTGCT+TGG | 0.442986 | 3.3:+58766483 | None:intergenic |
| ATTATTTACATAATTCCATA+AGG | 0.447464 | 3.3:-58767902 | MS.gene04987:intron |
| AATATGATCTCCAGGAAGTA+AGG | 0.453556 | 3.3:+58768264 | None:intergenic |
| GGACTCCATAAGCATGTTAG+AGG | 0.454900 | 3.3:-58770208 | MS.gene04987:intron |
| TGTAAGCAGCAATGCATTGA+AGG | 0.460226 | 3.3:+58764909 | None:intergenic |
| AGTTATTGCAGCTGAATCCT+TGG | 0.460876 | 3.3:-58768449 | MS.gene04987:CDS |
| TACTCTATCATCGGTGGAGT+TGG | 0.462273 | 3.3:-58766323 | MS.gene04987:CDS |
| GTCATCGCCAGTGCTATTAC+CGG | 0.478414 | 3.3:+58774450 | None:intergenic |
| TGTTTCAGAAGATGCTTGTA+GGG | 0.486266 | 3.3:+58768420 | None:intergenic |
| CCACTCTTGCAAGATGAAGT+AGG | 0.497488 | 3.3:+58767997 | None:intergenic |
| TCAGATACAATGTATCAAAT+CGG | 0.497907 | 3.3:-58771914 | MS.gene04987:CDS |
| GCAGTTGCTGTTCACAACCT+AGG | 0.501841 | 3.3:-58773146 | MS.gene04987:CDS |
| GCACCCTACCTTACATCATC+GGG | 0.501946 | 3.3:+58773409 | None:intergenic |
| CAAACCTGAATATGATCTCC+AGG | 0.506112 | 3.3:+58768256 | None:intergenic |
| ATCATCGGTGGAGTTGGCTA+AGG | 0.522722 | 3.3:-58766317 | MS.gene04987:CDS |
| TGCTTGTAGGGTCAGGGCCA+AGG | 0.526372 | 3.3:+58768432 | None:intergenic |
| GTATCTACAAGGAAAAGAAA+AGG | 0.529999 | 3.3:-58770250 | MS.gene04987:CDS |
| TGCACCCTACCTTACATCAT+CGG | 0.531142 | 3.3:+58773408 | None:intergenic |
| AAATTACCTGGGAGAGGGAA+CGG | 0.531256 | 3.3:+58770041 | None:intergenic |
| CAGTTGCTGTTCACAACCTA+GGG | 0.533918 | 3.3:-58773145 | MS.gene04987:CDS |
| GCCATAAAAGAAGCTAAAGA+AGG | 0.536015 | 3.3:-58774171 | MS.gene04987:CDS |
| TGGTTACACGTGCAGAAAAG+TGG | 0.536717 | 3.3:-58770097 | MS.gene04987:intron |
| AATCAAAAGAGCTTCTTGAA+AGG | 0.544707 | 3.3:-58768393 | MS.gene04987:intron |
| CCTACTTCATCTTGCAAGAG+TGG | 0.547747 | 3.3:-58767997 | MS.gene04987:CDS |
| GGAGTCCTTAATAATGATCT+CGG | 0.548174 | 3.3:+58770224 | None:intergenic |
| AAGACTCCGTTCCCTCTCCC+AGG | 0.551057 | 3.3:-58770047 | MS.gene04987:intron |
| CTACATTGGACAGCGGATGT+TGG | 0.556281 | 3.3:-58773117 | MS.gene04987:CDS |
| AGAAGATGCTTGTAGGGTCA+GGG | 0.556526 | 3.3:+58768426 | None:intergenic |
| TGTGCGTGGACATACTAGTA+AGG | 0.557790 | 3.3:-58766510 | MS.gene04987:CDS |
| GTTCAGGTTCTGTATCTACA+AGG | 0.560888 | 3.3:-58770261 | MS.gene04987:intron |
| CACGTGCAGAAAAGTGGCGA+AGG | 0.567042 | 3.3:-58770091 | MS.gene04987:intron |
| AACCTAGGGCAGTTCTACAT+TGG | 0.567557 | 3.3:-58773131 | MS.gene04987:CDS |
| GAAAGGGGATATTAATGTCA+AGG | 0.572405 | 3.3:-58764945 | MS.gene04987:CDS |
| ATCACTCAGTACTCTATCAT+CGG | 0.573902 | 3.3:-58766332 | MS.gene04987:CDS |
| GAATATAATGGAATTCTCAA+AGG | 0.576924 | 3.3:-58769017 | MS.gene04987:intron |
| ACGTCTTCCGGTAATAGCAC+TGG | 0.579677 | 3.3:-58774457 | MS.gene04987:CDS |
| AGAGTTGTACAGAGTCAAGA+AGG | 0.580160 | 3.3:-58773494 | MS.gene04987:CDS |
| AGCTGAGCTTGATATAGCAA+AGG | 0.580713 | 3.3:-58767925 | MS.gene04987:CDS |
| TATTAATGTCAAGGCTAAAG+AGG | 0.583256 | 3.3:-58764936 | MS.gene04987:CDS |
| TTGCATGAAGACGCGACATG+CGG | 0.588947 | 3.3:+58774132 | None:intergenic |
| TCTCTCTTGTAGATGTCTCG+AGG | 0.589360 | 3.3:-58768296 | MS.gene04987:intron |
| CATTAACACTGCGTTTGCAG+AGG | 0.600049 | 3.3:-58774494 | MS.gene04987:CDS |
| TCGTCTTGATGACGCTGAGA+TGG | 0.602862 | 3.3:-58769056 | MS.gene04987:CDS |
| AAAAGCTCTAGTTAGCTATG+AGG | 0.603879 | 3.3:-58773093 | MS.gene04987:intron |
| AATACAGGGATGGAAATCGT+TGG | 0.608242 | 3.3:-58768476 | MS.gene04987:intron |
| ACGTGCAGAAAAGTGGCGAA+GGG | 0.615807 | 3.3:-58770090 | MS.gene04987:intron |
| TCGAAGGAAACTAGTGTGCG+TGG | 0.623819 | 3.3:-58766524 | MS.gene04987:CDS |
| TTGATAGCTTCCAAATACAA+CGG | 0.634521 | 3.3:+58773454 | None:intergenic |
| GTCCAATGTAGAACTGCCCT+AGG | 0.634691 | 3.3:+58773129 | None:intergenic |
| TACAATGTATCAAATCGGCA+TGG | 0.639045 | 3.3:-58771909 | MS.gene04987:intron |
| CGTGCAGAAAAGTGGCGAAG+GGG | 0.647017 | 3.3:-58770089 | MS.gene04987:intron |
| ACTCAGTACTCTATCATCGG+TGG | 0.648209 | 3.3:-58766329 | MS.gene04987:CDS |
| GGCAGTTCTACATTGGACAG+CGG | 0.698235 | 3.3:-58773124 | MS.gene04987:CDS |
| ATGCATTGCTGCTTACAACG+AGG | 0.743840 | 3.3:-58764903 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 58764904 | 58774521 | 58764904 | ID=MS.gene04987 |
| chr3.3 | mRNA | 58764904 | 58774521 | 58764904 | ID=MS.gene04987.t1;Parent=MS.gene04987 |
| chr3.3 | exon | 58774417 | 58774521 | 58774417 | ID=MS.gene04987.t1.exon1;Parent=MS.gene04987.t1 |
| chr3.3 | CDS | 58774417 | 58774521 | 58774417 | ID=cds.MS.gene04987.t1;Parent=MS.gene04987.t1 |
| chr3.3 | exon | 58774125 | 58774229 | 58774125 | ID=MS.gene04987.t1.exon2;Parent=MS.gene04987.t1 |
| chr3.3 | CDS | 58774125 | 58774229 | 58774125 | ID=cds.MS.gene04987.t1;Parent=MS.gene04987.t1 |
| chr3.3 | exon | 58773418 | 58773518 | 58773418 | ID=MS.gene04987.t1.exon3;Parent=MS.gene04987.t1 |
| chr3.3 | CDS | 58773418 | 58773518 | 58773418 | ID=cds.MS.gene04987.t1;Parent=MS.gene04987.t1 |
| chr3.3 | exon | 58773094 | 58773172 | 58773094 | ID=MS.gene04987.t1.exon4;Parent=MS.gene04987.t1 |
| chr3.3 | CDS | 58773094 | 58773172 | 58773094 | ID=cds.MS.gene04987.t1;Parent=MS.gene04987.t1 |
| chr3.3 | exon | 58772092 | 58772103 | 58772092 | ID=MS.gene04987.t1.exon5;Parent=MS.gene04987.t1 |
| chr3.3 | CDS | 58772092 | 58772103 | 58772092 | ID=cds.MS.gene04987.t1;Parent=MS.gene04987.t1 |
| chr3.3 | exon | 58771910 | 58771975 | 58771910 | ID=MS.gene04987.t1.exon6;Parent=MS.gene04987.t1 |
| chr3.3 | CDS | 58771910 | 58771975 | 58771910 | ID=cds.MS.gene04987.t1;Parent=MS.gene04987.t1 |
| chr3.3 | exon | 58770209 | 58770277 | 58770209 | ID=MS.gene04987.t1.exon7;Parent=MS.gene04987.t1 |
| chr3.3 | CDS | 58770209 | 58770277 | 58770209 | ID=cds.MS.gene04987.t1;Parent=MS.gene04987.t1 |
| chr3.3 | exon | 58770048 | 58770104 | 58770048 | ID=MS.gene04987.t1.exon8;Parent=MS.gene04987.t1 |
| chr3.3 | CDS | 58770048 | 58770104 | 58770048 | ID=cds.MS.gene04987.t1;Parent=MS.gene04987.t1 |
| chr3.3 | exon | 58769018 | 58769095 | 58769018 | ID=MS.gene04987.t1.exon9;Parent=MS.gene04987.t1 |
| chr3.3 | CDS | 58769018 | 58769095 | 58769018 | ID=cds.MS.gene04987.t1;Parent=MS.gene04987.t1 |
| chr3.3 | exon | 58768394 | 58768491 | 58768394 | ID=MS.gene04987.t1.exon10;Parent=MS.gene04987.t1 |
| chr3.3 | CDS | 58768394 | 58768491 | 58768394 | ID=cds.MS.gene04987.t1;Parent=MS.gene04987.t1 |
| chr3.3 | exon | 58768261 | 58768306 | 58768261 | ID=MS.gene04987.t1.exon11;Parent=MS.gene04987.t1 |
| chr3.3 | CDS | 58768261 | 58768306 | 58768261 | ID=cds.MS.gene04987.t1;Parent=MS.gene04987.t1 |
| chr3.3 | exon | 58767903 | 58768030 | 58767903 | ID=MS.gene04987.t1.exon12;Parent=MS.gene04987.t1 |
| chr3.3 | CDS | 58767903 | 58768030 | 58767903 | ID=cds.MS.gene04987.t1;Parent=MS.gene04987.t1 |
| chr3.3 | exon | 58766484 | 58766592 | 58766484 | ID=MS.gene04987.t1.exon13;Parent=MS.gene04987.t1 |
| chr3.3 | CDS | 58766484 | 58766592 | 58766484 | ID=cds.MS.gene04987.t1;Parent=MS.gene04987.t1 |
| chr3.3 | exon | 58766297 | 58766359 | 58766297 | ID=MS.gene04987.t1.exon14;Parent=MS.gene04987.t1 |
| chr3.3 | CDS | 58766297 | 58766359 | 58766297 | ID=cds.MS.gene04987.t1;Parent=MS.gene04987.t1 |
| chr3.3 | exon | 58764904 | 58764966 | 58764904 | ID=MS.gene04987.t1.exon15;Parent=MS.gene04987.t1 |
| chr3.3 | CDS | 58764904 | 58764966 | 58764904 | ID=cds.MS.gene04987.t1;Parent=MS.gene04987.t1 |
| Gene Sequence |
| Protein sequence |
