Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30855.t1 | XP_013468302.1 | 93 | 129 | 9 | 0 | 1 | 129 | 227 | 355 | 2.40E-59 | 238 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30855.t1 | Q9LS48 | 45.1 | 102 | 53 | 1 | 25 | 126 | 217 | 315 | 2.1e-16 | 86.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30855.t1 | A0A072VJX8 | 93.0 | 129 | 9 | 0 | 1 | 129 | 227 | 355 | 1.7e-59 | 238.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049297 | MS.gene30855 | 0.86838 | 6.82E-66 | -1.69E-46 |
| MS.gene049378 | MS.gene30855 | 0.806995 | 6.27E-50 | -1.69E-46 |
| MS.gene049825 | MS.gene30855 | 0.817319 | 3.51E-52 | -1.69E-46 |
| MS.gene04987 | MS.gene30855 | 0.82031 | 7.34E-53 | -1.69E-46 |
| MS.gene050226 | MS.gene30855 | 0.841507 | 4.56E-58 | -1.69E-46 |
| MS.gene050227 | MS.gene30855 | 0.849898 | 2.41E-60 | -1.69E-46 |
| MS.gene050228 | MS.gene30855 | 0.860752 | 1.66E-63 | -1.69E-46 |
| MS.gene050277 | MS.gene30855 | 0.828678 | 7.87E-55 | -1.69E-46 |
| MS.gene050303 | MS.gene30855 | 0.890646 | 8.16E-74 | -1.69E-46 |
| MS.gene050330 | MS.gene30855 | -0.816356 | 5.77E-52 | -1.69E-46 |
| MS.gene050558 | MS.gene30855 | 0.802537 | 5.35E-49 | -1.69E-46 |
| MS.gene050623 | MS.gene30855 | -0.856217 | 3.73E-62 | -1.69E-46 |
| MS.gene050642 | MS.gene30855 | 0.886283 | 3.91E-72 | -1.69E-46 |
| MS.gene051257 | MS.gene30855 | 0.845771 | 3.30E-59 | -1.69E-46 |
| MS.gene051349 | MS.gene30855 | 0.84193 | 3.52E-58 | -1.69E-46 |
| MS.gene051633 | MS.gene30855 | 0.852944 | 3.31E-61 | -1.69E-46 |
| MS.gene051662 | MS.gene30855 | 0.824957 | 6.09E-54 | -1.69E-46 |
| MS.gene051983 | MS.gene30855 | 0.84287 | 1.99E-58 | -1.69E-46 |
| MS.gene052048 | MS.gene30855 | -0.807974 | 3.89E-50 | -1.69E-46 |
| MS.gene052176 | MS.gene30855 | 0.87637 | 1.47E-68 | -1.69E-46 |
| MS.gene052286 | MS.gene30855 | 0.829692 | 4.47E-55 | -1.69E-46 |
| MS.gene052302 | MS.gene30855 | 0.8655 | 5.65E-65 | -1.69E-46 |
| MS.gene052309 | MS.gene30855 | -0.868244 | 7.54E-66 | -1.69E-46 |
| MS.gene052358 | MS.gene30855 | -0.814868 | 1.24E-51 | -1.69E-46 |
| MS.gene052376 | MS.gene30855 | 0.87743 | 6.32E-69 | -1.69E-46 |
| MS.gene052600 | MS.gene30855 | 0.87406 | 9.07E-68 | -1.69E-46 |
| MS.gene052856 | MS.gene30855 | 0.819805 | 9.58E-53 | -1.69E-46 |
| MS.gene052858 | MS.gene30855 | 0.816184 | 6.30E-52 | -1.69E-46 |
| MS.gene052860 | MS.gene30855 | 0.808387 | 3.18E-50 | -1.69E-46 |
| MS.gene053080 | MS.gene30855 | 0.817242 | 3.65E-52 | -1.69E-46 |
| MS.gene053294 | MS.gene30855 | 0.833286 | 5.82E-56 | -1.69E-46 |
| MS.gene053836 | MS.gene30855 | 0.826805 | 2.22E-54 | -1.69E-46 |
| MS.gene054667 | MS.gene30855 | 0.801562 | 8.49E-49 | -1.69E-46 |
| MS.gene054701 | MS.gene30855 | 0.81154 | 6.65E-51 | -1.69E-46 |
| MS.gene054942 | MS.gene30855 | 0.866396 | 2.94E-65 | -1.69E-46 |
| MS.gene054983 | MS.gene30855 | 0.81496 | 1.18E-51 | -1.69E-46 |
| MS.gene055808 | MS.gene30855 | 0.822655 | 2.11E-53 | -1.69E-46 |
| MS.gene056069 | MS.gene30855 | -0.800523 | 1.38E-48 | -1.69E-46 |
| MS.gene056087 | MS.gene30855 | 0.807101 | 5.96E-50 | -1.69E-46 |
| MS.gene056091 | MS.gene30855 | 0.831814 | 1.35E-55 | -1.69E-46 |
| MS.gene056403 | MS.gene30855 | 0.801166 | 1.02E-48 | -1.69E-46 |
| MS.gene056506 | MS.gene30855 | 0.80452 | 2.08E-49 | -1.69E-46 |
| MS.gene056552 | MS.gene30855 | 0.814433 | 1.54E-51 | -1.69E-46 |
| MS.gene056605 | MS.gene30855 | 0.8082 | 3.48E-50 | -1.69E-46 |
| MS.gene056631 | MS.gene30855 | 0.83454 | 2.83E-56 | -1.69E-46 |
| MS.gene056979 | MS.gene30855 | 0.834426 | 3.02E-56 | -1.69E-46 |
| MS.gene057060 | MS.gene30855 | 0.826208 | 3.08E-54 | -1.69E-46 |
| MS.gene057079 | MS.gene30855 | 0.848646 | 5.37E-60 | -1.69E-46 |
| MS.gene057260 | MS.gene30855 | 0.804479 | 2.12E-49 | -1.69E-46 |
| MS.gene057555 | MS.gene30855 | 0.845636 | 3.59E-59 | -1.69E-46 |
| MS.gene05795 | MS.gene30855 | 0.813071 | 3.08E-51 | -1.69E-46 |
| MS.gene057967 | MS.gene30855 | 0.804266 | 2.35E-49 | -1.69E-46 |
| MS.gene058084 | MS.gene30855 | 0.821275 | 4.40E-53 | -1.69E-46 |
| MS.gene058182 | MS.gene30855 | 0.809152 | 2.18E-50 | -1.69E-46 |
| MS.gene058200 | MS.gene30855 | 0.827654 | 1.39E-54 | -1.69E-46 |
| MS.gene058201 | MS.gene30855 | 0.823192 | 1.58E-53 | -1.69E-46 |
| MS.gene058238 | MS.gene30855 | 0.810912 | 9.10E-51 | -1.69E-46 |
| MS.gene058399 | MS.gene30855 | 0.806436 | 8.23E-50 | -1.69E-46 |
| MS.gene058549 | MS.gene30855 | 0.811407 | 7.11E-51 | -1.69E-46 |
| MS.gene058749 | MS.gene30855 | 0.818435 | 1.96E-52 | -1.69E-46 |
| MS.gene058833 | MS.gene30855 | 0.822363 | 2.47E-53 | -1.69E-46 |
| MS.gene058906 | MS.gene30855 | 0.802963 | 4.37E-49 | -1.69E-46 |
| MS.gene058911 | MS.gene30855 | 0.801367 | 9.31E-49 | -1.69E-46 |
| MS.gene059249 | MS.gene30855 | 0.840552 | 8.12E-58 | -1.69E-46 |
| MS.gene05954 | MS.gene30855 | 0.857334 | 1.75E-62 | -1.69E-46 |
| MS.gene059569 | MS.gene30855 | 0.818182 | 2.24E-52 | -1.69E-46 |
| MS.gene05979 | MS.gene30855 | 0.805315 | 1.42E-49 | -1.69E-46 |
| MS.gene059830 | MS.gene30855 | 0.805816 | 1.11E-49 | -1.69E-46 |
| MS.gene05984 | MS.gene30855 | 0.806654 | 7.41E-50 | -1.69E-46 |
| MS.gene06001 | MS.gene30855 | 0.832924 | 7.16E-56 | -1.69E-46 |
| MS.gene060165 | MS.gene30855 | 0.833813 | 4.30E-56 | -1.69E-46 |
| MS.gene060297 | MS.gene30855 | 0.822262 | 2.60E-53 | -1.69E-46 |
| MS.gene060429 | MS.gene30855 | 0.800626 | 1.32E-48 | -1.69E-46 |
| MS.gene060572 | MS.gene30855 | 0.813597 | 2.36E-51 | -1.69E-46 |
| MS.gene060586 | MS.gene30855 | 0.824735 | 6.87E-54 | -1.69E-46 |
| MS.gene060619 | MS.gene30855 | 0.851792 | 7.05E-61 | -1.69E-46 |
| MS.gene060907 | MS.gene30855 | 0.805465 | 1.32E-49 | -1.69E-46 |
| MS.gene061040 | MS.gene30855 | -0.861405 | 1.05E-63 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30855.t1 | MTR_1g067600 | 93.023 | 129 | 9 | 0 | 1 | 129 | 227 | 355 | 8.82e-82 | 244 |
| MS.gene30855.t1 | MTR_7g100328 | 55.455 | 110 | 49 | 0 | 2 | 111 | 222 | 331 | 6.16e-43 | 144 |
| MS.gene30855.t1 | MTR_3g095070 | 47.500 | 80 | 42 | 0 | 31 | 110 | 229 | 308 | 1.06e-21 | 88.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30855.t1 | AT2G37400 | 46.774 | 124 | 63 | 1 | 1 | 121 | 206 | 329 | 6.17e-36 | 125 |
| MS.gene30855.t1 | AT3G53560 | 53.000 | 100 | 44 | 1 | 30 | 126 | 235 | 334 | 4.80e-34 | 121 |
| MS.gene30855.t1 | AT3G53560 | 53.000 | 100 | 44 | 1 | 30 | 126 | 283 | 382 | 9.14e-34 | 121 |
| MS.gene30855.t1 | AT5G02590 | 50.000 | 112 | 53 | 2 | 1 | 111 | 205 | 314 | 3.43e-31 | 113 |
| MS.gene30855.t1 | AT3G18420 | 41.739 | 115 | 64 | 1 | 12 | 126 | 204 | 315 | 4.89e-22 | 89.0 |
| MS.gene30855.t1 | AT4G39470 | 45.122 | 82 | 45 | 0 | 31 | 112 | 237 | 318 | 5.77e-21 | 86.7 |
| MS.gene30855.t1 | AT3G09490 | 41.758 | 91 | 53 | 0 | 16 | 106 | 204 | 294 | 1.05e-19 | 83.2 |
Find 20 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTCAAAATAATCTTTATA+AGG | 0.293256 | 1.1:+49811005 | None:intergenic |
| GGTGATGGAAGAGGATTATT+CGG | 0.343501 | 1.1:-49811180 | MS.gene30855:CDS |
| ACAACACCCTGACACAAATA+AGG | 0.396662 | 1.1:+49811104 | None:intergenic |
| GAAGTATAGAAGACTTGTTC+CGG | 0.415845 | 1.1:-49811036 | MS.gene30855:CDS |
| GGATTATTCGGGTGCATTGA+AGG | 0.439105 | 1.1:-49811168 | MS.gene30855:CDS |
| TTTAGACCTTATTTGTGTCA+GGG | 0.502448 | 1.1:-49811110 | MS.gene30855:CDS |
| GTGTTGTTTATAGTTTGCTT+AGG | 0.504927 | 1.1:-49811088 | MS.gene30855:CDS |
| TCTTTATAAGGATGACTCTC+CGG | 0.523440 | 1.1:+49811017 | None:intergenic |
| TTGTCGAAGAAGCTAGAGAA+AGG | 0.525668 | 1.1:-49810969 | MS.gene30855:CDS |
| GTGATGGAAGAGGATTATTC+GGG | 0.533262 | 1.1:-49811179 | MS.gene30855:CDS |
| GGGTGCATTGAAGGTGTATG+AGG | 0.551918 | 1.1:-49811159 | MS.gene30855:CDS |
| TAGCTTCTTCGACAAAACCT+TGG | 0.570727 | 1.1:+49810977 | None:intergenic |
| GCAGGTGAAGGTGATGGAAG+AGG | 0.577382 | 1.1:-49811189 | MS.gene30855:CDS |
| AATTGCGCAGGTGAAGGTGA+TGG | 0.587282 | 1.1:-49811195 | MS.gene30855:CDS |
| TTCGGAGTTGAAAGAGCCAA+TGG | 0.593450 | 1.1:-49811303 | MS.gene30855:CDS |
| GAGTTGAAAGAGCCAATGGA+GGG | 0.607803 | 1.1:-49811299 | MS.gene30855:CDS |
| TCGAAGAAGCTAGAGAAAGG+AGG | 0.633094 | 1.1:-49810966 | MS.gene30855:CDS |
| GGAGTTGAAAGAGCCAATGG+AGG | 0.637662 | 1.1:-49811300 | MS.gene30855:CDS |
| AAATAAGGTCTAAAATCACT+CGG | 0.653706 | 1.1:+49811119 | None:intergenic |
| GGTGTATGAGGAAATTGTGA+AGG | 0.676790 | 1.1:-49811147 | MS.gene30855:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCCTCAAAATAATCTTTATA+AGG | + | chr1.1:49811253-49811272 | None:intergenic | 20.0% |
| !!! | GTTTTTTGAAGAGAAAAAAA+TGG | - | chr1.1:49811009-49811028 | MS.gene30855:CDS | 20.0% |
| !!! | TCCTTATAAAGATTATTTTG+AGG | - | chr1.1:49811249-49811268 | MS.gene30855:CDS | 20.0% |
| !! | AAATAAGGTCTAAAATCACT+CGG | + | chr1.1:49811139-49811158 | None:intergenic | 25.0% |
| ! | TTTAGACCTTATTTGTGTCA+GGG | - | chr1.1:49811145-49811164 | MS.gene30855:CDS | 30.0% |
| !! | ATTTAAGCTTTTAATTGCGC+AGG | - | chr1.1:49811048-49811067 | MS.gene30855:CDS | 30.0% |
| !! | GTGTTGTTTATAGTTTGCTT+AGG | - | chr1.1:49811167-49811186 | MS.gene30855:CDS | 30.0% |
| !! | TTTTAGACCTTATTTGTGTC+AGG | - | chr1.1:49811144-49811163 | MS.gene30855:CDS | 30.0% |
| !!! | AATGGAGGGTTTTTTGAATA+GGG | - | chr1.1:49810970-49810989 | MS.gene30855:CDS | 30.0% |
| !!! | TTTTTTGAATAGGGTTGATG+CGG | - | chr1.1:49810979-49810998 | MS.gene30855:CDS | 30.0% |
| AAAAAATGGAGTCAGAAGCT+AGG | - | chr1.1:49811023-49811042 | MS.gene30855:CDS | 35.0% | |
| AAAAATGGAGTCAGAAGCTA+GGG | - | chr1.1:49811024-49811043 | MS.gene30855:CDS | 35.0% | |
| CTATTCAAAAAACCCTCCAT+TGG | + | chr1.1:49810971-49810990 | None:intergenic | 35.0% | |
| TCTTTATAAGGATGACTCTC+CGG | + | chr1.1:49811241-49811260 | None:intergenic | 35.0% | |
| ! | GAAGTATAGAAGACTTGTTC+CGG | - | chr1.1:49811219-49811238 | MS.gene30855:CDS | 35.0% |
| !!! | CAATGGAGGGTTTTTTGAAT+AGG | - | chr1.1:49810969-49810988 | MS.gene30855:CDS | 35.0% |
| !!! | TTATTTTGAGGATAACGCCA+AGG | - | chr1.1:49811261-49811280 | MS.gene30855:CDS | 35.0% |
| ACAACACCCTGACACAAATA+AGG | + | chr1.1:49811154-49811173 | None:intergenic | 40.0% | |
| GGTGTATGAGGAAATTGTGA+AGG | - | chr1.1:49811108-49811127 | MS.gene30855:CDS | 40.0% | |
| TAGCTTCTTCGACAAAACCT+TGG | + | chr1.1:49811281-49811300 | None:intergenic | 40.0% | |
| TTGTCGAAGAAGCTAGAGAA+AGG | - | chr1.1:49811286-49811305 | MS.gene30855:CDS | 40.0% | |
| ! | GTGATGGAAGAGGATTATTC+GGG | - | chr1.1:49811076-49811095 | MS.gene30855:CDS | 40.0% |
| !! | GGTGATGGAAGAGGATTATT+CGG | - | chr1.1:49811075-49811094 | MS.gene30855:CDS | 40.0% |
| GAGTTGAAAGAGCCAATGGA+GGG | - | chr1.1:49810956-49810975 | MS.gene30855:CDS | 45.0% | |
| GGATTATTCGGGTGCATTGA+AGG | - | chr1.1:49811087-49811106 | MS.gene30855:CDS | 45.0% | |
| TCGAAGAAGCTAGAGAAAGG+AGG | - | chr1.1:49811289-49811308 | MS.gene30855:CDS | 45.0% | |
| TTCGGAGTTGAAAGAGCCAA+TGG | - | chr1.1:49810952-49810971 | MS.gene30855:CDS | 45.0% | |
| !! | GCTTTTAATTGCGCAGGTGA+AGG | - | chr1.1:49811054-49811073 | MS.gene30855:CDS | 45.0% |
| AATTGCGCAGGTGAAGGTGA+TGG | - | chr1.1:49811060-49811079 | MS.gene30855:CDS | 50.0% | |
| GGAGTTGAAAGAGCCAATGG+AGG | - | chr1.1:49810955-49810974 | MS.gene30855:CDS | 50.0% | |
| ! | GGGTGCATTGAAGGTGTATG+AGG | - | chr1.1:49811096-49811115 | MS.gene30855:CDS | 50.0% |
| !! | GCAGGTGAAGGTGATGGAAG+AGG | - | chr1.1:49811066-49811085 | MS.gene30855:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 49810944 | 49811333 | 49810944 | ID=MS.gene30855 |
| chr1.1 | mRNA | 49810944 | 49811333 | 49810944 | ID=MS.gene30855.t1;Parent=MS.gene30855 |
| chr1.1 | exon | 49810944 | 49811333 | 49810944 | ID=MS.gene30855.t1.exon1;Parent=MS.gene30855.t1 |
| chr1.1 | CDS | 49810944 | 49811333 | 49810944 | ID=cds.MS.gene30855.t1;Parent=MS.gene30855.t1 |
| Gene Sequence |
| Protein sequence |
