AlfalfaGEDB | MS.gene073833

M. sativa cultivar XinJiangDaYe / MS.gene073833

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073833.t1	XP_013468302.1	94.3	159	9	0	9	167	197	355	5.10E-78	300.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073833.t1	Q9LS48	45.1	102	53	1	63	164	217	315	2.8e-16	86.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073833.t1	A0A072VJX8	94.3	159	9	0	9	167	197	355	3.6e-78	300.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049293	MS.gene073833	-0.807424	5.09E-50	-1.69E-46
MS.gene049297	MS.gene073833	0.876486	1.34E-68	-1.69E-46
MS.gene049322	MS.gene073833	0.807285	5.45E-50	-1.69E-46
MS.gene049756	MS.gene073833	0.804815	1.80E-49	-1.69E-46
MS.gene049825	MS.gene073833	0.814479	1.51E-51	-1.69E-46
MS.gene04987	MS.gene073833	0.831911	1.28E-55	-1.69E-46
MS.gene050226	MS.gene073833	0.836434	9.38E-57	-1.69E-46
MS.gene050227	MS.gene073833	0.8423	2.81E-58	-1.69E-46
MS.gene050228	MS.gene073833	0.853646	2.08E-61	-1.69E-46
MS.gene050277	MS.gene073833	0.834704	2.57E-56	-1.69E-46
MS.gene050303	MS.gene073833	0.883639	3.79E-71	-1.69E-46
MS.gene050330	MS.gene073833	-0.801931	7.13E-49	-1.69E-46
MS.gene050623	MS.gene073833	-0.853751	1.94E-61	-1.69E-46
MS.gene050642	MS.gene073833	0.884282	2.19E-71	-1.69E-46
MS.gene051257	MS.gene073833	0.837054	6.52E-57	-1.69E-46
MS.gene051349	MS.gene073833	0.81946	1.15E-52	-1.69E-46
MS.gene051451	MS.gene073833	0.812256	4.65E-51	-1.69E-46
MS.gene051633	MS.gene073833	0.837227	5.89E-57	-1.69E-46
MS.gene051662	MS.gene073833	0.819773	9.74E-53	-1.69E-46
MS.gene051983	MS.gene073833	0.841333	5.06E-58	-1.69E-46
MS.gene052176	MS.gene073833	0.864075	1.58E-64	-1.69E-46
MS.gene052256	MS.gene073833	0.802617	5.15E-49	-1.69E-46
MS.gene052286	MS.gene073833	0.82931	5.53E-55	-1.69E-46
MS.gene052302	MS.gene073833	0.86289	3.68E-64	-1.69E-46
MS.gene052309	MS.gene073833	-0.866102	3.64E-65	-1.69E-46
MS.gene052358	MS.gene073833	-0.802815	4.69E-49	-1.69E-46
MS.gene052376	MS.gene073833	0.871484	6.60E-67	-1.69E-46
MS.gene052586	MS.gene073833	0.800654	1.30E-48	-1.69E-46
MS.gene052600	MS.gene073833	0.85708	2.08E-62	-1.69E-46
MS.gene052856	MS.gene073833	0.833534	5.05E-56	-1.69E-46
MS.gene052858	MS.gene073833	0.814666	1.37E-51	-1.69E-46
MS.gene052860	MS.gene073833	0.805835	1.10E-49	-1.69E-46
MS.gene053080	MS.gene073833	0.814831	1.26E-51	-1.69E-46
MS.gene053216	MS.gene073833	0.805151	1.53E-49	-1.69E-46
MS.gene053294	MS.gene073833	0.838845	2.26E-57	-1.69E-46
MS.gene053836	MS.gene073833	0.829109	6.19E-55	-1.69E-46
MS.gene054537	MS.gene073833	0.803814	2.91E-49	-1.69E-46
MS.gene054701	MS.gene073833	0.823076	1.68E-53	-1.69E-46
MS.gene054942	MS.gene073833	0.861613	9.07E-64	-1.69E-46
MS.gene055808	MS.gene073833	0.834747	2.51E-56	-1.69E-46
MS.gene056087	MS.gene073833	0.803327	3.68E-49	-1.69E-46
MS.gene056091	MS.gene073833	0.823149	1.62E-53	-1.69E-46
MS.gene056403	MS.gene073833	0.810961	8.88E-51	-1.69E-46
MS.gene056605	MS.gene073833	0.822798	1.95E-53	-1.69E-46
MS.gene056631	MS.gene073833	0.836985	6.79E-57	-1.69E-46
MS.gene056979	MS.gene073833	0.837404	5.30E-57	-1.69E-46
MS.gene057060	MS.gene073833	0.822792	1.96E-53	-1.69E-46
MS.gene057079	MS.gene073833	0.844953	5.49E-59	-1.69E-46
MS.gene057121	MS.gene073833	0.800836	1.20E-48	-1.69E-46
MS.gene057260	MS.gene073833	0.806126	9.57E-50	-1.69E-46
MS.gene057555	MS.gene073833	0.847195	1.35E-59	-1.69E-46
MS.gene05795	MS.gene073833	0.805357	1.39E-49	-1.69E-46
MS.gene057967	MS.gene073833	0.802924	4.45E-49	-1.69E-46
MS.gene058084	MS.gene073833	0.82326	1.52E-53	-1.69E-46
MS.gene058182	MS.gene073833	0.810994	8.74E-51	-1.69E-46
MS.gene058200	MS.gene073833	0.820133	8.06E-53	-1.69E-46
MS.gene058201	MS.gene073833	0.816891	4.38E-52	-1.69E-46
MS.gene058238	MS.gene073833	0.825191	5.36E-54	-1.69E-46
MS.gene058399	MS.gene073833	0.814309	1.65E-51	-1.69E-46
MS.gene058749	MS.gene073833	0.809282	2.04E-50	-1.69E-46
MS.gene058833	MS.gene073833	0.829667	4.53E-55	-1.69E-46
MS.gene059249	MS.gene073833	0.821833	3.27E-53	-1.69E-46
MS.gene059317	MS.gene073833	0.804254	2.36E-49	-1.69E-46
MS.gene05954	MS.gene073833	0.851881	6.65E-61	-1.69E-46
MS.gene059569	MS.gene073833	0.805355	1.39E-49	-1.69E-46
MS.gene059830	MS.gene073833	0.808048	3.75E-50	-1.69E-46
MS.gene06001	MS.gene073833	0.840756	7.18E-58	-1.69E-46
MS.gene060165	MS.gene073833	0.835909	1.27E-56	-1.69E-46
MS.gene060297	MS.gene073833	0.838871	2.22E-57	-1.69E-46
MS.gene060586	MS.gene073833	0.836523	8.90E-57	-1.69E-46
MS.gene060619	MS.gene073833	0.846905	1.62E-59	-1.69E-46
MS.gene061040	MS.gene073833	-0.854922	8.91E-62	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073833.t1	MTR_1g067600	94.304	158	9	0	10	167	198	355	5.47e-105	305
MS.gene073833.t1	MTR_7g100328	54.110	146	63	1	8	149	186	331	2.38e-53	172
MS.gene073833.t1	MTR_3g095070	38.411	151	86	5	2	148	161	308	3.46e-23	94.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073833.t1	AT2G37400	48.052	154	77	1	9	159	176	329	4.42e-47	156
MS.gene073833.t1	AT3G53560	46.250	160	83	1	8	164	175	334	6.99e-46	153
MS.gene073833.t1	AT3G53560	46.250	160	83	1	8	164	223	382	1.62e-45	153
MS.gene073833.t1	AT5G02590	48.252	143	73	1	8	150	174	315	5.66e-43	145
MS.gene073833.t1	AT3G09490	37.681	138	86	0	7	144	157	294	4.99e-26	101
MS.gene073833.t1	AT3G18420	40.741	162	84	4	7	164	162	315	6.09e-26	100
MS.gene073833.t1	AT4G39470	34.014	147	93	2	6	150	174	318	8.81e-23	92.8

Find 28 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCAAATGCACTTCGGTCTT+TGG	0.265201	1.3:-47526016	MS.gene073833:intron
TCCTCAAAATAATCTTTATA+AGG	0.293256	1.3:+47525481	None:intergenic
GGTGATGGAAGAGGATTATT+CGG	0.343501	1.3:-47525656	MS.gene073833:CDS
ACAACACCCTGACACAAATA+AGG	0.396662	1.3:+47525580	None:intergenic
TCATAACGGTGAACATGAAT+TGG	0.408524	1.3:-47525878	MS.gene073833:CDS
GAAGTATAGAAGACTTGTTC+CGG	0.415845	1.3:-47525512	MS.gene073833:CDS
CGATAAGCTTCCGAGCTAAA+CGG	0.421270	1.3:+47525820	None:intergenic
GGATTATTCGGGTGCATTGA+AGG	0.439105	1.3:-47525644	MS.gene073833:CDS
GAACATGAATTGGCTAAGAA+AGG	0.494151	1.3:-47525868	MS.gene073833:CDS
TTTAGACCTTATTTGTGTCA+GGG	0.502448	1.3:-47525586	MS.gene073833:CDS
GTGTTGTTTATAGTTTGCTT+AGG	0.504927	1.3:-47525564	MS.gene073833:CDS
TCTTTATAAGGATGACTCTC+CGG	0.523440	1.3:+47525493	None:intergenic
TTGTCGAAGAAGCTAGAGAA+AGG	0.525668	1.3:-47525445	MS.gene073833:CDS
GTGATGGAAGAGGATTATTC+GGG	0.533262	1.3:-47525655	MS.gene073833:CDS
GGGTGCATTGAAGGTGTATG+AGG	0.551918	1.3:-47525635	MS.gene073833:CDS
TCACATTTGCACAGTCATAA+CGG	0.567156	1.3:-47525892	MS.gene073833:intron
TAGCTTCTTCGACAAAACCT+TGG	0.570727	1.3:+47525453	None:intergenic
GCAGGTGAAGGTGATGGAAG+AGG	0.577382	1.3:-47525665	MS.gene073833:CDS
AATTGCGCAGGTGAAGGTGA+TGG	0.587282	1.3:-47525671	MS.gene073833:CDS
TTCGGAGTTGAAAGAGCCAA+TGG	0.593450	1.3:-47525779	MS.gene073833:CDS
GCAGCAAGATCCGTTTAGCT+CGG	0.604756	1.3:-47525830	MS.gene073833:CDS
GAGTTGAAAGAGCCAATGGA+GGG	0.607803	1.3:-47525775	MS.gene073833:CDS
CCAACGATGCAAATGCACTT+CGG	0.615978	1.3:-47526023	None:intergenic
TCGAAGAAGCTAGAGAAAGG+AGG	0.633094	1.3:-47525442	MS.gene073833:CDS
GGAGTTGAAAGAGCCAATGG+AGG	0.637662	1.3:-47525776	MS.gene073833:CDS
TTTAGCTCGGAAGCTTATCG+CGG	0.648904	1.3:-47525817	MS.gene073833:CDS
AAATAAGGTCTAAAATCACT+CGG	0.653706	1.3:+47525595	None:intergenic
GGTGTATGAGGAAATTGTGA+AGG	0.676790	1.3:-47525623	MS.gene073833:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TCCTCAAAATAATCTTTATA+AGG	+	chr1.3:47525959-47525978	None:intergenic	20.0%
!!!	GTTTTTTGAAGAGAAAAAAA+TGG	-	chr1.3:47525715-47525734	MS.gene073833:CDS	20.0%
!!!	TCCTTATAAAGATTATTTTG+AGG	-	chr1.3:47525955-47525974	MS.gene073833:intron	20.0%
!!	AAATAAGGTCTAAAATCACT+CGG	+	chr1.3:47525845-47525864	None:intergenic	25.0%
	AGTAACAAATCAAATTCCTC+CGG	+	chr1.3:47525518-47525537	None:intergenic	30.0%
!	TTTAGACCTTATTTGTGTCA+GGG	-	chr1.3:47525851-47525870	MS.gene073833:CDS	30.0%
!!	ATTTAAGCTTTTAATTGCGC+AGG	-	chr1.3:47525754-47525773	MS.gene073833:CDS	30.0%
!!	GTGTTGTTTATAGTTTGCTT+AGG	-	chr1.3:47525873-47525892	MS.gene073833:CDS	30.0%
!!	TTTTAGACCTTATTTGTGTC+AGG	-	chr1.3:47525850-47525869	MS.gene073833:CDS	30.0%
!!!	AATGGAGGGTTTTTTGAATA+GGG	-	chr1.3:47525676-47525695	MS.gene073833:CDS	30.0%
!!!	TTTTTTGAATAGGGTTGATG+AGG	-	chr1.3:47525685-47525704	MS.gene073833:CDS	30.0%
	AAAAAATGGAGTCAGAAGCT+AGG	-	chr1.3:47525729-47525748	MS.gene073833:CDS	35.0%
	AAAAATGGAGTCAGAAGCTA+GGG	-	chr1.3:47525730-47525749	MS.gene073833:CDS	35.0%
	ACGATTAATCGAACTTGAAC+CGG	-	chr1.3:47525496-47525515	MS.gene073833:CDS	35.0%
	CTATTCAAAAAACCCTCCAT+TGG	+	chr1.3:47525677-47525696	None:intergenic	35.0%
	GAACATGAATTGGCTAAGAA+AGG	-	chr1.3:47525569-47525588	MS.gene073833:CDS	35.0%
	TCACATTTGCACAGTCATAA+CGG	-	chr1.3:47525545-47525564	MS.gene073833:CDS	35.0%
	TCATAACGGTGAACATGAAT+TGG	-	chr1.3:47525559-47525578	MS.gene073833:CDS	35.0%
	TCTTTATAAGGATGACTCTC+CGG	+	chr1.3:47525947-47525966	None:intergenic	35.0%
!	GAAGTATAGAAGACTTGTTC+CGG	-	chr1.3:47525925-47525944	MS.gene073833:intron	35.0%
!!!	CAATGGAGGGTTTTTTGAAT+AGG	-	chr1.3:47525675-47525694	MS.gene073833:CDS	35.0%
!!!	TTATTTTGAGGATAACGCCA+AGG	-	chr1.3:47525967-47525986	MS.gene073833:intron	35.0%
	ACAACACCCTGACACAAATA+AGG	+	chr1.3:47525860-47525879	None:intergenic	40.0%
	ATTAATCGAACTTGAACCGG+AGG	-	chr1.3:47525499-47525518	MS.gene073833:CDS	40.0%
	GGTGTATGAGGAAATTGTGA+AGG	-	chr1.3:47525814-47525833	MS.gene073833:CDS	40.0%
	TAGCTTCTTCGACAAAACCT+TGG	+	chr1.3:47525987-47526006	None:intergenic	40.0%
	TTGTCGAAGAAGCTAGAGAA+AGG	-	chr1.3:47525992-47526011	MS.gene073833:intron	40.0%
!	GTGATGGAAGAGGATTATTC+GGG	-	chr1.3:47525782-47525801	MS.gene073833:CDS	40.0%
!!	GGTGATGGAAGAGGATTATT+CGG	-	chr1.3:47525781-47525800	MS.gene073833:CDS	40.0%
	CGATAAGCTTCCGAGCTAAA+CGG	+	chr1.3:47525620-47525639	None:intergenic	45.0%
	GAGTTGAAAGAGCCAATGGA+GGG	-	chr1.3:47525662-47525681	MS.gene073833:CDS	45.0%
	GGATTATTCGGGTGCATTGA+AGG	-	chr1.3:47525793-47525812	MS.gene073833:CDS	45.0%
	TCGAAGAAGCTAGAGAAAGG+AGG	-	chr1.3:47525995-47526014	MS.gene073833:intron	45.0%
	TGCAAATGCACTTCGGTCTT+TGG	-	chr1.3:47525421-47525440	MS.gene073833:CDS	45.0%
	TTCGGAGTTGAAAGAGCCAA+TGG	-	chr1.3:47525658-47525677	MS.gene073833:CDS	45.0%
!	AGCTTATCGCGGTCTTTTGA+TGG	-	chr1.3:47525631-47525650	MS.gene073833:CDS	45.0%
!	TTTAGCTCGGAAGCTTATCG+CGG	-	chr1.3:47525620-47525639	MS.gene073833:CDS	45.0%
!!	GCTTTTAATTGCGCAGGTGA+AGG	-	chr1.3:47525760-47525779	MS.gene073833:CDS	45.0%
!!!	CGGTCTTTTGATGGCGAATT+CGG	-	chr1.3:47525640-47525659	MS.gene073833:CDS	45.0%
	AAGCGAAGCGATACGTGTTG+TGG	-	chr1.3:47525472-47525491	MS.gene073833:CDS	50.0%
	AATTGCGCAGGTGAAGGTGA+TGG	-	chr1.3:47525766-47525785	MS.gene073833:CDS	50.0%
	GCAGCAAGATCCGTTTAGCT+CGG	-	chr1.3:47525607-47525626	MS.gene073833:CDS	50.0%
	GGAGTTGAAAGAGCCAATGG+AGG	-	chr1.3:47525661-47525680	MS.gene073833:CDS	50.0%
	TGCACTTCGGTCTTTGGTTG+AGG	-	chr1.3:47525427-47525446	MS.gene073833:CDS	50.0%
!	GGGTGCATTGAAGGTGTATG+AGG	-	chr1.3:47525802-47525821	MS.gene073833:CDS	50.0%
!!	GCAGGTGAAGGTGATGGAAG+AGG	-	chr1.3:47525772-47525791	MS.gene073833:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	47525420	47526039	47525420	ID=MS.gene073833
chr1.3	mRNA	47525420	47526039	47525420	ID=MS.gene073833.t1;Parent=MS.gene073833
chr1.3	exon	47526017	47526039	47526017	ID=MS.gene073833.t1.exon1;Parent=MS.gene073833.t1
chr1.3	CDS	47526017	47526039	47526017	ID=cds.MS.gene073833.t1;Parent=MS.gene073833.t1
chr1.3	exon	47525420	47525900	47525420	ID=MS.gene073833.t1.exon2;Parent=MS.gene073833.t1
chr1.3	CDS	47525420	47525900	47525420	ID=cds.MS.gene073833.t1;Parent=MS.gene073833.t1

Gene Sequence

>MS.gene073833

ATGCAAATGCACTTCGGTCTTTGGTTGAGGAAAACGTGAAAGCACGCAAACTAAGCGAAGCGATACGTGTTGTGGAACGATTAATCGAACTTGAACCGGAGGAATTTGATTTGTTACTGCTAAAATCACATTTGCACAGTCATAACGGTGAACATGAATTGGCTAAGAAAGGATTTGAGTTTACTCTGCAGCAAGATCCGTTTAGCTCGGAAGCTTATCGCGGTCTTTTGATGGCGAATTCGGAGTTGAAAGAGCCAATGGAGGGTTTTTTGAATAGGGTTGATGAGGTAGTGAAGTTTTTTGAAGAGAAAAAAATGGAGTCAGAAGCTAGGGAATTTAAGCTTTTAATTGCGCAGGTGAAGGTGATGGAAGAGGATTATTCGGGTGCATTGAAGGTGTATGAGGAAATTGTGAAGGAAGAACCGAGTGATTTTAGACCTTATTTGTGTCAGGGTGTTGTTTATAGTTTGCTTAGGAAGAATGATGAAGCTCAGAAACAGTTTGAGAAGTATAGAAGACTTGTTCCGGAGAGTCATCCTTATAAAGATTATTTTGAGGATAACGCCAAGGTTTTGTCGAAGAAGCTAGAGAAAGGAGGAATAGAAGAAGCAAAGATTTGA

Protein sequence

>MS.gene073833.t1

MQMHFGLCHNGEHELAKKGFEFTLQQDPFSSEAYRGLLMANSELKEPMEGFLNRVDEVVKFFEEKKMESEAREFKLLIAQVKVMEEDYSGALKVYEEIVKEEPSDFRPYLCQGVVYSLLRKNDEAQKQFEKYRRLVPESHPYKDYFEDNAKVLSKKLEKGGIEEAKI