AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07942


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07942.t1 MTR_6g038170 67.361 144 15 5 1 124 11 142 1.04e-54 167
MS.gene07942.t1 MTR_6g038180 79.487 39 8 0 1 39 1 39 2.57e-17 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 0 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATTAAAAAAAAAAAATTC+GGG - 42963:2743-2762 MS.gene07942:intron 10.0%
!!! ACTAGTTTTTTTTTTTTTTT+AGG + 42963:2913-2932 None:intergenic 10.0%
!!! CTAGTTTTTTTTTTTTTTTA+GGG + 42963:2912-2931 None:intergenic 10.0%
!! AATACTAAATGTTAGTATGT+TGG - 42963:2966-2985 MS.gene07942:intron 20.0%
!!! TTCAGATTAATTTGTTAATG+AGG - 42963:3024-3043 MS.gene07942:intron 20.0%
!!! TTTGTTAATGAGGTTTTTTT+TGG - 42963:3034-3053 MS.gene07942:intron 20.0%
! ACAAATTAATCTGAATGACT+TGG + 42963:3019-3038 None:intergenic 25.0%
! AGGTTAAACACAAATCATTT+AGG - 42963:3161-3180 MS.gene07942:intron 25.0%
!! ATATTCTAGAGAAAGATTGT+TGG + 42963:3211-3230 None:intergenic 25.0%
!! GAAAATCCAAAATCGAATTT+TGG - 42963:3183-3202 MS.gene07942:intron 25.0%
!! TTATCAGCACTTTGATAATT+AGG - 42963:3282-3301 MS.gene07942:CDS 25.0%
!!! AATTTTTGGAGTAAAGAACA+TGG + 42963:2550-2569 None:intergenic 25.0%
AAGCAAATGTTACAAATGAG+AGG - 42963:2500-2519 MS.gene07942:CDS 30.0%
AGAAATTCTGAATTAATCCC+TGG + 42963:3419-3438 None:intergenic 30.0%
AGAAGATCAGATATTAGTAC+TGG - 42963:3353-3372 MS.gene07942:CDS 30.0%
CTCATTAACAATTGTACCTT+CGG + 42963:3143-3162 None:intergenic 30.0%
GAACTTTCTTCATATGTCTA+GGG - 42963:2575-2594 MS.gene07942:CDS 30.0%
GTAACATTTGCTTCTTCATT+TGG + 42963:2493-2512 None:intergenic 30.0%
TCATTAACAATTGTACCTTC+GGG + 42963:3142-3161 None:intergenic 30.0%
TCTTTACTCCAAAAATTCCT+TGG - 42963:2553-2572 MS.gene07942:CDS 30.0%
TGGATGGTAAAGACAAAAAT+TGG + 42963:2530-2549 None:intergenic 30.0%
! AGAAAGTTCCAAGGAATTTT+TGG + 42963:2564-2583 None:intergenic 30.0%
! ATGTTAGTATGTTGGTGAAT+AGG - 42963:2974-2993 MS.gene07942:intron 30.0%
! TTGGAACCAAAATTCGATTT+TGG + 42963:3192-3211 None:intergenic 30.0%
! TTGTGACGTTCCAATTTTAT+CGG - 42963:3111-3130 MS.gene07942:intron 30.0%
AGAAGATCAGATCTTAGTAC+TGG - 42963:3317-3336 MS.gene07942:CDS 35.0%
CATTAACAATTGTACCTTCG+GGG + 42963:3141-3160 None:intergenic 35.0%
CGTCACAAAAAAGATTAGCA+TGG + 42963:3099-3118 None:intergenic 35.0%
GACATATGAAGAAAGTTCCA+AGG + 42963:2573-2592 None:intergenic 35.0%
GGAACTTTCTTCATATGTCT+AGG - 42963:2574-2593 MS.gene07942:CDS 35.0%
GTACTAAGATCTGATCTTCT+TGG + 42963:3317-3336 None:intergenic 35.0%
! ACTTTGATAATTAGGAGTGG+CGG - 42963:3290-3309 MS.gene07942:CDS 35.0%
! AGCACTTTGATAATTAGGAG+TGG - 42963:3287-3306 MS.gene07942:CDS 35.0%
! CGAAGGTACAATTGTTAATG+AGG - 42963:3141-3160 MS.gene07942:intron 35.0%
! TCAGATATTAGTACTGGAAG+AGG - 42963:3359-3378 MS.gene07942:CDS 35.0%
! TTAGTATGTTGGTGAATAGG+TGG - 42963:2977-2996 MS.gene07942:intron 35.0%
! TTTGGAGTAAAGAACATGGA+TGG + 42963:2546-2565 None:intergenic 35.0%
!! CTGAATGACTTGGTTCTATA+AGG + 42963:3009-3028 None:intergenic 35.0%
GAATGTTCAACGAGTGATGA+TGG - 42963:2652-2671 MS.gene07942:intron 40.0%
GATGCAGAAGAGAATCTTGA+TGG - 42963:2610-2629 MS.gene07942:CDS 40.0%
GGAAGATCAGGTCTTAGTAT+TGG - 42963:3386-3405 MS.gene07942:CDS 40.0%
TCAAGATTCTCTTCTGCATC+AGG + 42963:2610-2629 None:intergenic 40.0%
! GATATTAGTACTGGAAGAGG+TGG - 42963:3362-3381 MS.gene07942:CDS 40.0%
! TCAGATCTTAGTACTGGAAG+AGG - 42963:3323-3342 MS.gene07942:CDS 40.0%
! TCAGGTCTTAGTATTGGAAG+AGG - 42963:3392-3411 MS.gene07942:CDS 40.0%
! TTAGTATTGGAAGAGGTCCA+GGG - 42963:3399-3418 MS.gene07942:CDS 40.0%
ATTAGCATGGCTCCTAGACA+AGG + 42963:3086-3105 None:intergenic 45.0%
TCGGGGACATCCGATAAAAT+TGG + 42963:3124-3143 None:intergenic 45.0%
TGTGCATGTCATCCTTGTCT+AGG - 42963:3071-3090 MS.gene07942:intron 45.0%
! ATTAGTACTGGAAGAGGTGG+AGG - 42963:3365-3384 MS.gene07942:CDS 45.0%
! ATTTTATCGGATGTCCCCGA+AGG - 42963:3124-3143 MS.gene07942:intron 45.0%
! CTTAGTATTGGAAGAGGTCC+AGG - 42963:3398-3417 MS.gene07942:CDS 45.0%
! GATCTTAGTACTGGAAGAGG+TGG - 42963:3326-3345 MS.gene07942:CDS 45.0%
!! ATGATTAAAAAAAAAAAATT+CGG - 42963:2742-2761 MS.gene07942:intron 5.0%
GGAAGAGGTGGAGGAAGATC+AGG - 42963:3374-3393 MS.gene07942:CDS 55.0%


Chromosome Type Strat End Strand Name
42963 gene 2475 3442 2475 ID=MS.gene07942
42963 mRNA 2475 3442 2475 ID=MS.gene07942.t1;Parent=MS.gene07942
42963 exon 3233 3442 3233 ID=MS.gene07942.t1.exon1;Parent=MS.gene07942.t1
42963 CDS 3233 3442 3233 ID=cds.MS.gene07942.t1;Parent=MS.gene07942.t1
42963 exon 2475 2639 2475 ID=MS.gene07942.t1.exon2;Parent=MS.gene07942.t1
42963 CDS 2475 2639 2475 ID=cds.MS.gene07942.t1;Parent=MS.gene07942.t1
Gene Sequence

>MS.gene07942

ATGGAAGAAATATCACCAAATGAAGAAGCAAATGTTACAAATGAGAGGAGTGCCAATTTTTGTCTTTACCATCCATGTTCTTTACTCCAAAAATTCCTTGGAACTTTCTTCATATGTCTAGGGTTTCAAATTCCTGATGCAGAAGAGAATCTTGATGGCAAAGTTCTTGCATGTGAAGAATGTTCAACGAGTGATGATGGTCAAAGTTTTGTAAGCTTTTCTCTCTTTTTAATTTTTTTTGACTGAATTCTTTCTTTTTAATTTGAGATGATTAAAAAAAAAAAATTCGGGTATTCATTTGTTTCTTATTTCTTCAATGAGAATCATCTTGTAAGTAAAAAAAATGATTAATTTGACAAAACTAAAATCAATTAAAAAAAAATAAAAAATAAGAGTTGATATTTTTAACACATCTATAATTAGTTTAGTTATTTCCCTAAAAAAAAAAAAAAACTAGTTATTATGACTATTTTGAACAACTAAAACTTGTTAATACTAAATGTTAGTATGTTGGTGAATAGGTGGAATCAACCTTATAGAACCAAGTCATTCAGATTAATTTGTTAATGAGGTTTTTTTTGGACAATTTCTTGATTTGTGCATGTCATCCTTGTCTAGGAGCCATGCTAATCTTTTTTGTGACGTTCCAATTTTATCGGATGTCCCCGAAGGTACAATTGTTAATGAGGTTAAACACAAATCATTTAGGAAAATCCAAAATCGAATTTTGGTTCCAACAATCTTTCTCTAGAATATAAGCAAAGTATTCACACATTCTGATTTGTATTTTCTTTTTCTTACAGCAACTTATCAGCACTTTGATAATTAGGAGTGGCGGACCAAGAAGATCAGATCTTAGTACTGGAAGAGGTGGACGAAGAAGATCAGATATTAGTACTGGAAGAGGTGGAGGAAGATCAGGTCTTAGTATTGGAAGAGGTCCAGGGATTAATTCAGAATTTCTCTAA

Protein sequence

>MS.gene07942.t1

MEEISPNEEANVTNERSANFCLYHPCSLLQKFLGTFFICLGFQIPDAEENLDGKVLACEECSTSDDGQSFQLISTLIIRSGGPRRSDLSTGRGGRRRSDISTGRGGGRSGLSIGRGPGINSEFL