AlfalfaGEDB | MS.gene35428

M. sativa cultivar XinJiangDaYe / MS.gene35428

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35428.t1	RHN75471.1	98.3	116	2	0	1	116	94	209	2.50E-60	241.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35428.t1	G7IHF3	98.3	116	2	0	1	116	57	172	1.8e-60	241.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049260	MS.gene35428	0.812498	4.11E-51	-1.69E-46
MS.gene049382	MS.gene35428	0.830473	2.88E-55	-1.69E-46
MS.gene049695	MS.gene35428	0.800149	1.65E-48	-1.69E-46
MS.gene050020	MS.gene35428	0.862368	5.33E-64	-1.69E-46
MS.gene05016	MS.gene35428	0.807766	4.31E-50	-1.69E-46
MS.gene050206	MS.gene35428	0.811258	7.66E-51	-1.69E-46
MS.gene050226	MS.gene35428	0.802224	6.21E-49	-1.69E-46
MS.gene050438	MS.gene35428	0.815329	9.77E-52	-1.69E-46
MS.gene050442	MS.gene35428	0.800821	1.20E-48	-1.69E-46
MS.gene050516	MS.gene35428	0.804709	1.90E-49	-1.69E-46
MS.gene050584	MS.gene35428	0.893032	9.17E-75	-1.69E-46
MS.gene050585	MS.gene35428	0.877663	5.25E-69	-1.69E-46
MS.gene050586	MS.gene35428	0.886691	2.74E-72	-1.69E-46
MS.gene050587	MS.gene35428	0.86024	2.37E-63	-1.69E-46
MS.gene050762	MS.gene35428	0.825563	4.38E-54	-1.69E-46
MS.gene050763	MS.gene35428	0.825896	3.65E-54	-1.69E-46
MS.gene050807	MS.gene35428	0.849959	2.31E-60	-1.69E-46
MS.gene050808	MS.gene35428	0.816572	5.16E-52	-1.69E-46
MS.gene050815	MS.gene35428	0.815321	9.82E-52	-1.69E-46
MS.gene05143	MS.gene35428	0.819313	1.24E-52	-1.69E-46
MS.gene051626	MS.gene35428	0.866915	2.01E-65	-1.69E-46
MS.gene051789	MS.gene35428	0.90429	1.43E-79	-1.69E-46
MS.gene052142	MS.gene35428	0.842758	2.13E-58	-1.69E-46
MS.gene052187	MS.gene35428	0.813381	2.63E-51	-1.69E-46
MS.gene052255	MS.gene35428	0.807939	3.96E-50	-1.69E-46
MS.gene052323	MS.gene35428	0.80041	1.46E-48	-1.69E-46
MS.gene052665	MS.gene35428	0.834392	3.08E-56	-1.69E-46
MS.gene052698	MS.gene35428	0.825742	3.97E-54	-1.69E-46
MS.gene052860	MS.gene35428	0.808439	3.09E-50	-1.69E-46
MS.gene052967	MS.gene35428	0.843664	1.22E-58	-1.69E-46
MS.gene053074	MS.gene35428	0.924398	7.43E-90	-1.69E-46
MS.gene053184	MS.gene35428	0.820194	7.80E-53	-1.69E-46
MS.gene053204	MS.gene35428	0.859182	4.94E-63	-1.69E-46
MS.gene053383	MS.gene35428	0.809122	2.21E-50	-1.69E-46
MS.gene053555	MS.gene35428	0.803888	2.81E-49	-1.69E-46
MS.gene053563	MS.gene35428	0.806634	7.48E-50	-1.69E-46
MS.gene053630	MS.gene35428	0.833111	6.43E-56	-1.69E-46
MS.gene053836	MS.gene35428	0.806849	6.74E-50	-1.69E-46
MS.gene054184	MS.gene35428	0.853264	2.68E-61	-1.69E-46
MS.gene054185	MS.gene35428	0.856406	3.29E-62	-1.69E-46
MS.gene054214	MS.gene35428	0.8059	1.07E-49	-1.69E-46
MS.gene054379	MS.gene35428	0.834698	2.58E-56	-1.69E-46
MS.gene05456	MS.gene35428	0.87227	3.62E-67	-1.69E-46
MS.gene054596	MS.gene35428	0.893132	8.35E-75	-1.69E-46
MS.gene055085	MS.gene35428	0.818533	1.87E-52	-1.69E-46
MS.gene055133	MS.gene35428	0.818083	2.36E-52	-1.69E-46
MS.gene055207	MS.gene35428	0.845183	4.76E-59	-1.69E-46
MS.gene055359	MS.gene35428	0.863116	3.13E-64	-1.69E-46
MS.gene055604	MS.gene35428	0.811813	5.80E-51	-1.69E-46
MS.gene056069	MS.gene35428	-0.816508	5.33E-52	-1.69E-46
MS.gene056760	MS.gene35428	0.823872	1.10E-53	-1.69E-46
MS.gene057395	MS.gene35428	0.801617	8.27E-49	-1.69E-46
MS.gene057750	MS.gene35428	0.83518	1.95E-56	-1.69E-46
MS.gene057754	MS.gene35428	0.858354	8.73E-63	-1.69E-46
MS.gene057869	MS.gene35428	0.812514	4.08E-51	-1.69E-46
MS.gene058168	MS.gene35428	0.892331	1.75E-74	-1.69E-46
MS.gene058906	MS.gene35428	0.804316	2.29E-49	-1.69E-46
MS.gene058982	MS.gene35428	0.814837	1.26E-51	-1.69E-46
MS.gene059006	MS.gene35428	0.909702	4.25E-82	-1.69E-46
MS.gene059124	MS.gene35428	0.904438	1.23E-79	-1.69E-46
MS.gene059128	MS.gene35428	0.826165	3.15E-54	-1.69E-46
MS.gene059421	MS.gene35428	0.810899	9.16E-51	-1.69E-46
MS.gene059713	MS.gene35428	0.81292	3.32E-51	-1.69E-46
MS.gene060320	MS.gene35428	0.815809	7.64E-52	-1.69E-46
MS.gene060470	MS.gene35428	0.818568	1.83E-52	-1.69E-46
MS.gene060476	MS.gene35428	0.826206	3.08E-54	-1.69E-46
MS.gene060480	MS.gene35428	0.807321	5.35E-50	-1.69E-46
MS.gene060513	MS.gene35428	0.805164	1.52E-49	-1.69E-46
MS.gene060998	MS.gene35428	0.816928	4.29E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35428.t1	MTR_2g086680	98.276	116	2	0	1	116	57	172	5.92e-83	240

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35428.t1	AT2G27385	45.133	113	51	3	4	116	61	162	2.14e-24	91.3
MS.gene35428.t1	AT2G27385	45.133	113	51	3	4	116	72	173	3.72e-24	91.3
MS.gene35428.t1	AT2G27385	45.133	113	51	3	4	116	72	173	3.72e-24	91.3
MS.gene35428.t1	AT5G22430	44.915	118	53	5	4	116	62	172	1.20e-20	82.0

Find 30 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTTCACAATTTGGGAGAAT+TGG	0.256434	2.1:+15518713	None:intergenic
CTTCCTTTCTTCCCCATCAT+TGG	0.318190	2.1:-15518529	MS.gene35428:CDS
CTTGCCTCCAGAGTGGGGTT+TGG	0.331819	2.1:-15518569	MS.gene35428:CDS
AATTGCCATGCAAAACTTCT+TGG	0.358597	2.1:-15518769	MS.gene35428:CDS
GGTTTCTTCTTGAAGCATAA+AGG	0.387581	2.1:+15518734	None:intergenic
CTTGAAGCATAAAGGTTAGT+TGG	0.400325	2.1:+15518742	None:intergenic
TTTCCCCTTGCCTCCAGAGT+GGG	0.435282	2.1:-15518575	MS.gene35428:CDS
TTTGCATGGCAATTATCATA+AGG	0.435517	2.1:+15518778	None:intergenic
AGAAAATGATGGCTCCTTCA+TGG	0.464326	2.1:-15518830	MS.gene35428:CDS
TGATCATGGTATTCCAATGA+TGG	0.466506	2.1:+15518516	None:intergenic
AAACCCCACTCTGGAGGCAA+GGG	0.479581	2.1:+15518571	None:intergenic
CCAAATTGTGAAGGGAAAAG+AGG	0.515617	2.1:-15518704	MS.gene35428:CDS
ATTCCAATGATGGGGAAGAA+AGG	0.527851	2.1:+15518526	None:intergenic
TGCCATGCAAAACTTCTTGG+TGG	0.529749	2.1:-15518766	MS.gene35428:CDS
CTTGGTGCCAAACCCCACTC+TGG	0.534566	2.1:+15518562	None:intergenic
CAAACCCCACTCTGGAGGCA+AGG	0.536034	2.1:+15518570	None:intergenic
AGTGAAGAAACTGAGAGGAG+TGG	0.538559	2.1:+15518663	None:intergenic
TTCCCCTTGCCTCCAGAGTG+GGG	0.538731	2.1:-15518574	MS.gene35428:CDS
GATCATGGTATTCCAATGAT+GGG	0.544352	2.1:+15518517	None:intergenic
CCAATTCTCCCAAATTGTGA+AGG	0.561971	2.1:-15518713	MS.gene35428:CDS
GCCATGCAAAACTTCTTGGT+GGG	0.568791	2.1:-15518765	MS.gene35428:CDS
ATTTCCCCTTGCCTCCAGAG+TGG	0.574643	2.1:-15518576	MS.gene35428:CDS
AAAGGAAGATAATAACTACT+TGG	0.580003	2.1:+15518544	None:intergenic
AACCCCACTCTGGAGGCAAG+GGG	0.597617	2.1:+15518572	None:intergenic
ATGGCAACTACAGAAAATGA+TGG	0.598455	2.1:-15518841	MS.gene35428:CDS
CAATTCTCCCAAATTGTGAA+GGG	0.607626	2.1:-15518712	MS.gene35428:CDS
TGTGGAAAGATCAACCATGA+AGG	0.637832	2.1:+15518816	None:intergenic
GGTGCCAAACCCCACTCTGG+AGG	0.646400	2.1:+15518565	None:intergenic
ATCATGGTATTCCAATGATG+GGG	0.661584	2.1:+15518518	None:intergenic
CAAGAAGTGAAGAAACTGAG+AGG	0.684855	2.1:+15518658	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAACTGAGAAAAATGAGTT+TGG	-	chr2.1:15518750-15518769	MS.gene35428:CDS	25.0%
!	AAAGGAAGATAATAACTACT+TGG	+	chr2.1:15518825-15518844	None:intergenic	25.0%
!!!	AGTTTTGCATTCTTTGTTTT+GGG	+	chr2.1:15518736-15518755	None:intergenic	25.0%
	TTTGCATGGCAATTATCATA+AGG	+	chr2.1:15518591-15518610	None:intergenic	30.0%
!	TATCATAAGGAAGTTTTGCA+TGG	+	chr2.1:15518578-15518597	None:intergenic	30.0%
!!	CAGTTTTGCATTCTTTGTTT+TGG	+	chr2.1:15518737-15518756	None:intergenic	30.0%
!!!	GTTTTGCATTCTTTGTTTTG+GGG	+	chr2.1:15518735-15518754	None:intergenic	30.0%
!!!	TTTTGCATTCTTTGTTTTGG+GGG	+	chr2.1:15518734-15518753	None:intergenic	30.0%
	AATTGCCATGCAAAACTTCT+TGG	-	chr2.1:15518597-15518616	MS.gene35428:CDS	35.0%
	ATGGCAACTACAGAAAATGA+TGG	-	chr2.1:15518525-15518544	MS.gene35428:CDS	35.0%
	CAATTCTCCCAAATTGTGAA+GGG	-	chr2.1:15518654-15518673	MS.gene35428:CDS	35.0%
	CTTGAAGCATAAAGGTTAGT+TGG	+	chr2.1:15518627-15518646	None:intergenic	35.0%
	GGTTTCTTCTTGAAGCATAA+AGG	+	chr2.1:15518635-15518654	None:intergenic	35.0%
!	CTCTTTTCCCTTCACAATTT+GGG	+	chr2.1:15518664-15518683	None:intergenic	35.0%
	AGAAAATGATGGCTCCTTCA+TGG	-	chr2.1:15518536-15518555	MS.gene35428:CDS	40.0%
	CAAGAAGTGAAGAAACTGAG+AGG	+	chr2.1:15518711-15518730	None:intergenic	40.0%
	CCAAATTGTGAAGGGAAAAG+AGG	-	chr2.1:15518662-15518681	MS.gene35428:CDS	40.0%
	CCAATTCTCCCAAATTGTGA+AGG	-	chr2.1:15518653-15518672	MS.gene35428:CDS	40.0%
	CCTTCACAATTTGGGAGAAT+TGG	+	chr2.1:15518656-15518675	None:intergenic	40.0%
	TGTGGAAAGATCAACCATGA+AGG	+	chr2.1:15518553-15518572	None:intergenic	40.0%
!	ATTCCAATGATGGGGAAGAA+AGG	+	chr2.1:15518843-15518862	None:intergenic	40.0%
!	CCTCTTTTCCCTTCACAATT+TGG	+	chr2.1:15518665-15518684	None:intergenic	40.0%
	AGTGAAGAAACTGAGAGGAG+TGG	+	chr2.1:15518706-15518725	None:intergenic	45.0%
	CTTCCTTTCTTCCCCATCAT+TGG	-	chr2.1:15518837-15518856	MS.gene35428:CDS	45.0%
	GCCATGCAAAACTTCTTGGT+GGG	-	chr2.1:15518601-15518620	MS.gene35428:CDS	45.0%
	TGCCATGCAAAACTTCTTGG+TGG	-	chr2.1:15518600-15518619	MS.gene35428:CDS	45.0%
!	AGGAAGTTTTGCATGGCTTG+TGG	+	chr2.1:15518571-15518590	None:intergenic	45.0%
!	GCCCACCAAGAAGTTTTGCA+TGG	+	chr2.1:15518605-15518624	None:intergenic	50.0%
	ATTTCCCCTTGCCTCCAGAG+TGG	-	chr2.1:15518790-15518809	MS.gene35428:CDS	55.0%
	TTTCCCCTTGCCTCCAGAGT+GGG	-	chr2.1:15518791-15518810	MS.gene35428:CDS	55.0%
!	AAACCCCACTCTGGAGGCAA+GGG	+	chr2.1:15518798-15518817	None:intergenic	55.0%
	CTTGCCTCCAGAGTGGGGTT+TGG	-	chr2.1:15518797-15518816	MS.gene35428:CDS	60.0%
	TTCCCCTTGCCTCCAGAGTG+GGG	-	chr2.1:15518792-15518811	MS.gene35428:CDS	60.0%
!	AACCCCACTCTGGAGGCAAG+GGG	+	chr2.1:15518797-15518816	None:intergenic	60.0%
!	CAAACCCCACTCTGGAGGCA+AGG	+	chr2.1:15518799-15518818	None:intergenic	60.0%
!!	CTTGGTGCCAAACCCCACTC+TGG	+	chr2.1:15518807-15518826	None:intergenic	60.0%
	GGTGCCAAACCCCACTCTGG+AGG	+	chr2.1:15518804-15518823	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	15518519	15518869	15518519	ID=MS.gene35428
chr2.1	mRNA	15518519	15518869	15518519	ID=MS.gene35428.t1;Parent=MS.gene35428
chr2.1	exon	15518519	15518869	15518519	ID=MS.gene35428.t1.exon1;Parent=MS.gene35428.t1
chr2.1	CDS	15518519	15518869	15518519	ID=cds.MS.gene35428.t1;Parent=MS.gene35428.t1

Gene Sequence	>MS.gene35428 ATGGCAATGGCAACTACAGAAAATGATGGCTCCTTCATGGTTGATCTTTCCACAAGCCATGCAAAACTTCCTTATGATAATTGCCATGCAAAACTTCTTGGTGGGCCAACTAACCTTTATGCTTCAAGAAGAAACCAATTCTCCCAAATTGTGAAGGGAAAAGAGGAAAATAACTACACACTATCCACTCCTCTCAGTTTCTTCACTTCTTGCCCCCAAAACAAAGAATGCAAAACTGAGAAAAATGAGTTTGGTTCATCAAAAAGCATCAATTTCCCCTTGCCTCCAGAGTGGGGTTTGGCACCAAGTAGTTATTATCTTCCTTTCTTCCCCATCATTGGAATACCATGA
Protein sequence	>MS.gene35428.t1 MAMATTENDGSFMVDLSTSHAKLPYDNCHAKLLGGPTNLYASRRNQFSQIVKGKEENNYTLSTPLSFFTSCPQNKECKTEKNEFGSSKSINFPLPPEWGLAPSSYYLPFFPIIGIP