AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23012


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23012.t1 MTR_7g108130 95.288 191 8 1 1 190 1 191 2.94e-131 366
MS.gene23012.t1 MTR_4g118810 42.373 177 91 2 1 166 1 177 5.95e-44 145
MS.gene23012.t1 MTR_2g020060 44.242 165 85 2 31 189 26 189 8.68e-39 131
MS.gene23012.t1 MTR_4g118800 34.783 161 95 4 26 181 26 181 2.74e-22 89.4
MS.gene23012.t1 MTR_8g089300 30.409 171 109 4 2 166 4 170 3.15e-20 84.0
MS.gene23012.t1 MTR_4g081880 30.189 159 106 3 21 177 22 177 1.20e-17 77.0
MS.gene23012.t1 MTR_0097s0070 29.452 146 97 2 45 184 44 189 1.02e-15 72.4
MS.gene23012.t1 MTR_5g041900 29.221 154 104 3 26 177 26 176 7.89e-15 69.7
MS.gene23012.t1 MTR_0004s0210 28.571 161 105 3 21 181 1 151 8.87e-15 68.9
MS.gene23012.t1 MTR_7g083120 29.787 141 93 3 31 169 33 169 1.34e-13 66.2
MS.gene23012.t1 MTR_7g011090 28.125 160 110 3 20 177 20 176 1.51e-13 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23012.t1 AT4G15630 39.267 191 109 2 1 185 1 190 1.79e-42 141
MS.gene23012.t1 AT4G15620 37.895 190 111 2 1 184 1 189 3.20e-39 132
MS.gene23012.t1 AT4G15610 30.994 171 114 3 1 167 1 171 3.69e-23 91.7
MS.gene23012.t1 AT3G06390 34.074 135 85 2 29 159 38 172 1.59e-17 77.0
MS.gene23012.t1 AT5G44550 32.500 160 100 3 32 184 22 180 2.22e-16 73.9
MS.gene23012.t1 AT4G15610 31.532 111 70 2 63 167 5 115 2.53e-16 72.4
MS.gene23012.t1 AT4G20390 36.090 133 79 3 32 158 22 154 9.05e-16 72.4
MS.gene23012.t1 AT5G15290 27.891 147 99 3 21 164 21 163 1.92e-15 71.2
MS.gene23012.t1 AT1G14160 28.221 163 114 3 26 187 49 209 1.43e-12 63.9
MS.gene23012.t1 AT4G16442 27.119 177 116 4 16 185 1 171 2.83e-11 60.1
MS.gene23012.t1 AT2G27370 29.193 161 109 3 26 184 60 217 6.05e-11 59.7

Find 33 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTACTATCATTGTGGGTTT+TGG 0.265618 7.2:-6789961 MS.gene23012:CDS
GATGTGTTGCTTAGGGTTCT+TGG 0.291243 7.2:-6790003 MS.gene23012:CDS
CATCCACACTTGTCTTTGAT+TGG 0.294482 7.2:+6790079 None:intergenic
TTCATATGCTGCAATTTCTT+TGG 0.300522 7.2:-6788618 MS.gene23012:CDS
GAGAGTGAGCCACAAGTAAA+AGG 0.319910 7.2:+6788357 None:intergenic
GCTTTGGTCCTATCTATCAT+TGG 0.335452 7.2:-6788392 MS.gene23012:CDS
CACTGCACCAGCAGCTCCAT+TGG 0.363063 7.2:+6788502 None:intergenic
TCTAGTTTGATCTATAATAA+TGG 0.389846 7.2:+6788324 None:intergenic
AATGCTAGACCTAGTTATAA+TGG 0.390891 7.2:-6788540 MS.gene23012:CDS
GTGTTTATGATGTGTTGCTT+AGG 0.399152 7.2:-6790011 MS.gene23012:CDS
CTCACTGCTACTATCATTGT+GGG 0.403424 7.2:-6789967 MS.gene23012:CDS
GCTGGTGCAGTGGGAGTGTT+AGG 0.413062 7.2:-6788491 MS.gene23012:CDS
TTCTCAGCCAATGGAGCTGC+TGG 0.424568 7.2:-6788509 MS.gene23012:CDS
TCACAGCGATGTGAAGGTTA+TGG 0.427097 7.2:-6790052 MS.gene23012:CDS
AACAGTCATGTGACGACAAT+AGG 0.431776 7.2:+6788415 None:intergenic
TGTTTATGATGTGTTGCTTA+GGG 0.433057 7.2:-6790010 MS.gene23012:CDS
CAGTGGAACCAATGATAGAT+AGG 0.464992 7.2:+6788384 None:intergenic
TCGTCACATGACTGTTGCTT+TGG 0.473880 7.2:-6788408 MS.gene23012:CDS
TTCTTGGTGACAAATGCTAT+AGG 0.474689 7.2:-6788644 MS.gene23012:CDS
TCTCACTGCTACTATCATTG+TGG 0.489600 7.2:-6789968 MS.gene23012:CDS
AAGGAAACTCGCACGTGCAA+TGG 0.499701 7.2:-6788463 MS.gene23012:CDS
CAATCAAAGACAAGTGTGGA+TGG 0.535899 7.2:-6790078 MS.gene23012:CDS
GCATTACTCTTCTCAGCCAA+TGG 0.539824 7.2:-6788518 MS.gene23012:CDS
AATGGAGCTGCTGGTGCAGT+GGG 0.549722 7.2:-6788500 MS.gene23012:CDS
ATTCAAAGCTACTGCTAAGT+GGG 0.553173 7.2:-6789902 MS.gene23012:CDS
CAATGGAGCTGCTGGTGCAG+TGG 0.564486 7.2:-6788501 MS.gene23012:CDS
AATTCAAAGCTACTGCTAAG+TGG 0.570996 7.2:-6789903 MS.gene23012:CDS
GTGGGAGTGTTAGGACAGAA+AGG 0.578933 7.2:-6788482 MS.gene23012:CDS
TCAACAATTGCAAGAACCAG+TGG 0.593273 7.2:-6788590 MS.gene23012:CDS
TGGAGTTCACAGCGATGTGA+AGG 0.594175 7.2:-6790058 MS.gene23012:CDS
AGAGTAATGCCATTATAACT+AGG 0.597714 7.2:+6788531 None:intergenic
AAACCAATCAAAGACAAGTG+TGG 0.653780 7.2:-6790082 MS.gene23012:CDS
CACAAGTAAAAGGAAGACAG+TGG 0.729118 7.2:+6788367 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTTACTAAAAATAAAATAA+AGG + chr7.2:6789045-6789064 None:intergenic 10.0%
!! TTTAAAAAATTGTAAAAGAA+AGG + chr7.2:6788784-6788803 None:intergenic 10.0%
!!! TTTTTCTTCAAAAAAATTAA+TGG + chr7.2:6789361-6789380 None:intergenic 10.0%
!! AAAAAAATATAAAACAAACG+AGG + chr7.2:6789698-6789717 None:intergenic 15.0%
!! AATACATTTATATTCCAAAT+AGG + chr7.2:6789510-6789529 None:intergenic 15.0%
!! ATACATTTATATTCCAAATA+GGG + chr7.2:6789509-6789528 None:intergenic 15.0%
!! TTCATTAACAAATGTTATAA+GGG - chr7.2:6788723-6788742 MS.gene23012:intron 15.0%
!! TTTCATTAACAAATGTTATA+AGG - chr7.2:6788722-6788741 MS.gene23012:intron 15.0%
!!! AAATGTTTTAAGAAAGTAAA+TGG - chr7.2:6788968-6788987 MS.gene23012:intron 15.0%
!!! ATATATTGATTGATTACTAA+TGG + chr7.2:6789133-6789152 None:intergenic 15.0%
!!! ATTTAGTTTTTGAAATGTTA+AGG - chr7.2:6788598-6788617 MS.gene23012:CDS 15.0%
!!! GTATATAGTTTTGAAAATTT+AGG + chr7.2:6788946-6788965 None:intergenic 15.0%
!!! TATAATATGTTAGTTGATAA+CGG - chr7.2:6789064-6789083 MS.gene23012:intron 15.0%
!!! TATTTGGAATATAAATGTAT+TGG - chr7.2:6789509-6789528 MS.gene23012:intron 15.0%
!!! TATTTTTTTGAAAATGTTTC+TGG - chr7.2:6788844-6788863 MS.gene23012:intron 15.0%
!!! TTTAGTTTTTGAAATGTTAA+GGG - chr7.2:6788599-6788618 MS.gene23012:CDS 15.0%
!! AAAAAATTGTAAAAGAAAGG+TGG + chr7.2:6788781-6788800 None:intergenic 20.0%
!! AAGATAATGTAGTGAATATT+TGG + chr7.2:6788894-6788913 None:intergenic 20.0%
!! AGATAATGTAGTGAATATTT+GGG + chr7.2:6788893-6788912 None:intergenic 20.0%
!! ATAAATAAAGTGATGTTGAT+TGG + chr7.2:6789463-6789482 None:intergenic 20.0%
!! TAATGACAGACTTTAATTAA+TGG + chr7.2:6789391-6789410 None:intergenic 20.0%
!! TCAAAAACTAAATTATCTAG+TGG + chr7.2:6788591-6788610 None:intergenic 20.0%
!!! ATGTATTGGTATTTTTTAAG+AGG - chr7.2:6789523-6789542 MS.gene23012:intron 20.0%
!!! CATCACTTTATTTATCATAT+TGG - chr7.2:6789468-6789487 MS.gene23012:intron 20.0%
!!! GATGAATTACTTTTTTTGAA+AGG + chr7.2:6789272-6789291 None:intergenic 20.0%
!!! TTTCTTAATTAGTGCTATTA+GGG + chr7.2:6788676-6788695 None:intergenic 20.0%
!!! TTTTCTTAATTAGTGCTATT+AGG + chr7.2:6788677-6788696 None:intergenic 20.0%
!! TGTTTTGTATTTTCCTTCAT+AGG - chr7.2:6788635-6788654 MS.gene23012:CDS 25.0%
!! TTGATTGGTTAAGCTTTAAT+GGG + chr7.2:6789448-6789467 None:intergenic 25.0%
!!! TTTTATAAAGTGTCGAATAC+AGG + chr7.2:6789577-6789596 None:intergenic 25.0%
!!! TTTTTAAGAGGTGTGATATT+TGG - chr7.2:6789535-6789554 MS.gene23012:intron 25.0%
AATAAAAACACTATGTGCTG+AGG + chr7.2:6789014-6789033 None:intergenic 30.0%
AATGCTAGACCTAGTTATAA+TGG - chr7.2:6789872-6789891 MS.gene23012:intron 30.0%
ACAATTTCACAAAGATGCAT+TGG - chr7.2:6789300-6789319 MS.gene23012:intron 30.0%
AGAGTAATGCCATTATAACT+AGG + chr7.2:6789884-6789903 None:intergenic 30.0%
CATTTATATTCCAAATAGGG+AGG + chr7.2:6789506-6789525 None:intergenic 30.0%
TTCATATGCTGCAATTTCTT+TGG - chr7.2:6789794-6789813 MS.gene23012:intron 30.0%
! ATTCTCTCTTAGGTTTTTCT+TGG - chr7.2:6789752-6789771 MS.gene23012:intron 30.0%
! GTTGATTGGTTAAGCTTTAA+TGG + chr7.2:6789449-6789468 None:intergenic 30.0%
! TATTTGGGATAAAGAGAAGA+TGG + chr7.2:6788878-6788897 None:intergenic 30.0%
!! CAGTCACTTTGAAATTACTT+AGG + chr7.2:6789727-6789746 None:intergenic 30.0%
!! TGTTTATGATGTGTTGCTTA+GGG - chr7.2:6788402-6788421 MS.gene23012:CDS 30.0%
!!! ATAGTGTTGATGTCAGATTT+TGG - chr7.2:6789671-6789690 MS.gene23012:intron 30.0%
!!! TAGTGTTGATGTCAGATTTT+GGG - chr7.2:6789672-6789691 MS.gene23012:intron 30.0%
AAACCAATCAAAGACAAGTG+TGG - chr7.2:6788330-6788349 MS.gene23012:CDS 35.0%
AAGGTGGTAGTACAAATGAA+AGG + chr7.2:6788765-6788784 None:intergenic 35.0%
AATTCAAAGCTACTGCTAAG+TGG - chr7.2:6788509-6788528 MS.gene23012:CDS 35.0%
ATGTACTTAAGCTTGTGAGT+AGG - chr7.2:6789172-6789191 MS.gene23012:intron 35.0%
ATTCAAAGCTACTGCTAAGT+GGG - chr7.2:6788510-6788529 MS.gene23012:CDS 35.0%
TATATTCCAAATAGGGAGGA+AGG + chr7.2:6789502-6789521 None:intergenic 35.0%
TGTACTTAAGCTTGTGAGTA+GGG - chr7.2:6789173-6789192 MS.gene23012:intron 35.0%
! CTTGTTAACTTGACCTATGA+AGG + chr7.2:6788651-6788670 None:intergenic 35.0%
! GACTGACTTTATTCTCTCTT+AGG - chr7.2:6789742-6789761 MS.gene23012:intron 35.0%
! TTCTTGGTGACAAATGCTAT+AGG - chr7.2:6789768-6789787 MS.gene23012:intron 35.0%
!! GTGTTTATGATGTGTTGCTT+AGG - chr7.2:6788401-6788420 MS.gene23012:CDS 35.0%
!!! TAGTGCTGTTTTATTTCCAC+TGG + chr7.2:6789841-6789860 None:intergenic 35.0%
AACAGTCATGTGACGACAAT+AGG + chr7.2:6790000-6790019 None:intergenic 40.0%
CAATCAAAGACAAGTGTGGA+TGG - chr7.2:6788334-6788353 MS.gene23012:CDS 40.0%
CACAAGTAAAAGGAAGACAG+TGG + chr7.2:6790048-6790067 None:intergenic 40.0%
CAGTGGAACCAATGATAGAT+AGG + chr7.2:6790031-6790050 None:intergenic 40.0%
CATCCACACTTGTCTTTGAT+TGG + chr7.2:6788336-6788355 None:intergenic 40.0%
CTCACTGCTACTATCATTGT+GGG - chr7.2:6788445-6788464 MS.gene23012:CDS 40.0%
GCTTTGGTCCTATCTATCAT+TGG - chr7.2:6790020-6790039 MS.gene23012:CDS 40.0%
TCAACAATTGCAAGAACCAG+TGG - chr7.2:6789822-6789841 MS.gene23012:intron 40.0%
TCTCACTGCTACTATCATTG+TGG - chr7.2:6788444-6788463 MS.gene23012:CDS 40.0%
TGTGAGTAGGGTTTAGTAGT+TGG - chr7.2:6789185-6789204 MS.gene23012:intron 40.0%
! CACTGTCTTCCTTTTACTTG+TGG - chr7.2:6790046-6790065 MS.gene23012:CDS 40.0%
! GCTACTATCATTGTGGGTTT+TGG - chr7.2:6788451-6788470 MS.gene23012:CDS 40.0%
!! TATCATTGTGGGTTTTGGCA+AGG - chr7.2:6788456-6788475 MS.gene23012:CDS 40.0%
CTAGTGGTAGCATAGTAGAG+AGG + chr7.2:6788575-6788594 None:intergenic 45.0%
GAGAGTGAGCCACAAGTAAA+AGG + chr7.2:6790058-6790077 None:intergenic 45.0%
GCATTACTCTTCTCAGCCAA+TGG - chr7.2:6789894-6789913 MS.gene23012:CDS 45.0%
TCACAGCGATGTGAAGGTTA+TGG - chr7.2:6788360-6788379 MS.gene23012:CDS 45.0%
! TCGTCACATGACTGTTGCTT+TGG - chr7.2:6790004-6790023 MS.gene23012:CDS 45.0%
!! GATGTGTTGCTTAGGGTTCT+TGG - chr7.2:6788409-6788428 MS.gene23012:CDS 45.0%
AAGGAAACTCGCACGTGCAA+TGG - chr7.2:6789949-6789968 MS.gene23012:CDS 50.0%
GTAGCTCCTTCCTCCCTATT+TGG - chr7.2:6789493-6789512 MS.gene23012:intron 50.0%
TGGAGTTCACAGCGATGTGA+AGG - chr7.2:6788354-6788373 MS.gene23012:CDS 50.0%
!! GGGTTTTGGCAAGGAGACTA+AGG - chr7.2:6788465-6788484 MS.gene23012:CDS 50.0%
!! GTGGGAGTGTTAGGACAGAA+AGG - chr7.2:6789930-6789949 MS.gene23012:CDS 50.0%
AATGGAGCTGCTGGTGCAGT+GGG - chr7.2:6789912-6789931 MS.gene23012:CDS 55.0%
TTCTCAGCCAATGGAGCTGC+TGG - chr7.2:6789903-6789922 MS.gene23012:CDS 55.0%
CAATGGAGCTGCTGGTGCAG+TGG - chr7.2:6789911-6789930 MS.gene23012:CDS 60.0%
CACTGCACCAGCAGCTCCAT+TGG + chr7.2:6789913-6789932 None:intergenic 60.0%
!! GCTGGTGCAGTGGGAGTGTT+AGG - chr7.2:6789921-6789940 MS.gene23012:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 6788325 6790109 6788325 ID=MS.gene23012
chr7.2 mRNA 6788325 6790109 6788325 ID=MS.gene23012.t1;Parent=MS.gene23012
chr7.2 exon 6789883 6790109 6789883 ID=MS.gene23012.t1.exon1;Parent=MS.gene23012.t1
chr7.2 CDS 6789883 6790109 6789883 ID=cds.MS.gene23012.t1;Parent=MS.gene23012.t1
chr7.2 exon 6788325 6788670 6788325 ID=MS.gene23012.t1.exon2;Parent=MS.gene23012.t1
chr7.2 CDS 6788325 6788670 6788325 ID=cds.MS.gene23012.t1;Parent=MS.gene23012.t1
Gene Sequence

>MS.gene23012

ATGGGAAACCAATCAAAGACAAGTGTGGATGGAGTTCACAGCGATGTGAAGGTTATGGAGATGAGAACTAGAAGTAGTGTTTATGATGTGTTGCTTAGGGTTCTTGGTTTCTTGCTTACTCTCACTGCTACTATCATTGTGGGTTTTGGCAAGGAGACTAAGGTCATTTCATATGCTTCATTGCAATTCAAAGCTACTGCTAAGTGGGAATATATGTCTGCTATTGTGTAAGCTCATCACATCTCTTCCTCTCTACTATGCTACCACTAGATAATTTAGTTTTTGAAATGTTAAGGGCAAGTAGTTCTCATGTTTTGTATTTTCCTTCATAGGTCAAGTTAACAAGTGCCCTAATAGCACTAATTAAGAAAACAAAAAATTGAAATTTCTAAATTTATTTCATTAACAAATGTTATAAGGGTACTCTTTAGCATTTTCCTTTCATTTGTACTACCACCTTTCTTTTACAATTTTTTAAAACAGATCTTACATTTTTAAAATAAAAGATGTCAAAATATATATTTTTTTGAAAATGTTTCTGGTTTGCTTTCCATCTTCTCTTTATCCCAAATATTCACTACATTATCTTTTATCTCATTGATAACAATTTAAAATAAACCTAAATTTTCAAAACTATATACTGAAATGTTTTAAGAAAGTAAATGGAGTCTAAATATGTTTTGAATCCTCAGCACATAGTGTTTTTATTTTTTTGAACCTTTATTTTATTTTTAGTAACTATAATATGTTAGTTGATAACGGTATATTTTTCATTCAAAAAGTTTTTTTTAATATATAAGAAAATCCATTAGTAATCAATCAATATATAAGATGTGACGCAAATAGTATGTACTTAAGCTTGTGAGTAGGGTTTAGTAGTTGGTAAAAACAGTTGTTCTACTATATCAACATCTTAAATGAATTTTAATTAACTCGTGATATCTCCTTTCAAAAAAAGTAATTCATCATATCTCTACAATTTCACAAAGATGCATTGGTTAACAAAAAAAAATTATCTATTTCAAAAATAAAACCATTAATTTTTTTGAAGAAAAAAATAAAACCATTAATTAAAGTCTGTCATTATCAAATCTAAAACTAAACAAATCAAATCAGCTGACCCATTAAAGCTTAACCAATCAACATCACTTTATTTATCATATTGGTCGTAGCTCCTTCCTCCCTATTTGGAATATAAATGTATTGGTATTTTTTAAGAGGTGTGATATTTGGACATTCTCAATTCAAACCTGTATTCGACACTTTATAAAATATAACTGAGATGTGTGTAATGTTAGAAGCGATGAGAGAAAAAATAATATAAAAGTCGAAAACAATATATACATATAGTGTTGATGTCAGATTTTGGGCCTCGTTTGTTTTATATTTTTTTTGTCTTCCTAAGTAATTTCAAAGTGACTGACTTTATTCTCTCTTAGGTTTTTCTTGGTGACAAATGCTATAGGATGTTCATATGCTGCAATTTCTTTGGTGATTTCAACAATTGCAAGAACCAGTGGAAATAAAACAGCACTATTGATGATAACAATGCTAGACCTAGTTATAATGGCATTACTCTTCTCAGCCAATGGAGCTGCTGGTGCAGTGGGAGTGTTAGGACAGAAAGGAAACTCGCACGTGCAATGGATGAAAGTGTGCAATGTATTTGATGCCTATTGTCGTCACATGACTGTTGCTTTGGTCCTATCTATCATTGGTTCCACTGTCTTCCTTTTACTTGTGGCTCACTCTCTTTTCAAACTCCATTATTATAGATCAAACTAG

Protein sequence

>MS.gene23012.t1

MGNQSKTSVDGVHSDVKVMEMRTRSSVYDVLLRVLGFLLTLTATIIVGFGKETKVISYASLQFKATAKWEYMSAIVFFLVTNAIGCSYAAISLVISTIARTSGNKTALLMITMLDLVIMALLFSANGAAGAVGVLGQKGNSHVQWMKVCNVFDAYCRHMTVALVLSIIGSTVFLLLVAHSLFKLHYYRSN