AlfalfaGEDB | MS.gene75321

M. sativa cultivar XinJiangDaYe / MS.gene75321

KEGG: -
GO: GO:0002376 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0009605 GO:0009607 GO:0043207 GO:0045087 GO:0050896 GO:0051704 GO:0051707 GO:0098542
PFAM: PF17232.5

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75321.t1	XP_013451912.1	68.3	104	12	4	1	84	11	113	1.80E-24	122.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75321.t1	A0A072UJE8	68.3	104	12	4	1	84	11	113	1.3e-24	122.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049260	MS.gene75321	0.82907	6.32E-55	-1.69E-46
MS.gene050020	MS.gene75321	0.837287	5.68E-57	-1.69E-46
MS.gene050139	MS.gene75321	0.81475	1.31E-51	-1.69E-46
MS.gene050206	MS.gene75321	0.815393	9.46E-52	-1.69E-46
MS.gene050438	MS.gene75321	0.815768	7.81E-52	-1.69E-46
MS.gene050584	MS.gene75321	0.822182	2.72E-53	-1.69E-46
MS.gene050586	MS.gene75321	0.828193	1.03E-54	-1.69E-46
MS.gene050780	MS.gene75321	0.806991	6.29E-50	-1.69E-46
MS.gene050807	MS.gene75321	0.810643	1.04E-50	-1.69E-46
MS.gene050815	MS.gene75321	0.810101	1.36E-50	-1.69E-46
MS.gene051265	MS.gene75321	0.807476	4.96E-50	-1.69E-46
MS.gene051626	MS.gene75321	0.875794	2.33E-68	-1.69E-46
MS.gene051789	MS.gene75321	0.894037	3.59E-75	-1.69E-46
MS.gene051860	MS.gene75321	0.811839	5.73E-51	-1.69E-46
MS.gene052142	MS.gene75321	0.811012	8.66E-51	-1.69E-46
MS.gene052178	MS.gene75321	0.808402	3.15E-50	-1.69E-46
MS.gene052187	MS.gene75321	0.821757	3.41E-53	-1.69E-46
MS.gene052286	MS.gene75321	0.802699	4.96E-49	-1.69E-46
MS.gene052698	MS.gene75321	0.803381	3.58E-49	-1.69E-46
MS.gene052967	MS.gene75321	0.84352	1.33E-58	-1.69E-46
MS.gene053074	MS.gene75321	0.913912	3.54E-84	-1.69E-46
MS.gene053184	MS.gene75321	0.80824	3.41E-50	-1.69E-46
MS.gene053204	MS.gene75321	0.840026	1.11E-57	-1.69E-46
MS.gene053630	MS.gene75321	0.824657	7.17E-54	-1.69E-46
MS.gene053836	MS.gene75321	0.808947	2.41E-50	-1.69E-46
MS.gene054011	MS.gene75321	0.800193	1.62E-48	-1.69E-46
MS.gene054184	MS.gene75321	0.889131	3.19E-73	-1.69E-46
MS.gene054185	MS.gene75321	0.8918	2.85E-74	-1.69E-46
MS.gene054214	MS.gene75321	0.80219	6.31E-49	-1.69E-46
MS.gene05456	MS.gene75321	0.852499	4.44E-61	-1.69E-46
MS.gene054596	MS.gene75321	0.871793	5.21E-67	-1.69E-46
MS.gene055133	MS.gene75321	0.818911	1.53E-52	-1.69E-46
MS.gene055207	MS.gene75321	0.841748	3.94E-58	-1.69E-46
MS.gene055358	MS.gene75321	0.807211	5.65E-50	-1.69E-46
MS.gene055359	MS.gene75321	0.840168	1.02E-57	-1.69E-46
MS.gene055604	MS.gene75321	0.807412	5.12E-50	-1.69E-46
MS.gene056069	MS.gene75321	-0.812794	3.54E-51	-1.69E-46
MS.gene056760	MS.gene75321	0.802178	6.35E-49	-1.69E-46
MS.gene057060	MS.gene75321	0.804322	2.28E-49	-1.69E-46
MS.gene057395	MS.gene75321	0.803905	2.79E-49	-1.69E-46
MS.gene057750	MS.gene75321	0.802689	4.98E-49	-1.69E-46
MS.gene057754	MS.gene75321	0.841015	6.14E-58	-1.69E-46
MS.gene058168	MS.gene75321	0.88743	1.44E-72	-1.69E-46
MS.gene059006	MS.gene75321	0.904767	8.70E-80	-1.69E-46
MS.gene059124	MS.gene75321	0.857043	2.14E-62	-1.69E-46
MS.gene059421	MS.gene75321	0.829635	4.61E-55	-1.69E-46
MS.gene060476	MS.gene75321	0.831889	1.29E-55	-1.69E-46
MS.gene061049	MS.gene75321	0.804614	1.98E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75321.t1	MTR_6g038170	67.361	144	15	5	1	124	11	142	1.04e-54	167
MS.gene75321.t1	MTR_6g038180	79.487	39	8	0	1	39	1	39	2.57e-17	71.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 26 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTATCAGCACTTTGATAATT+AGG	0.202851	6.3:-30670708	MS.gene75321:CDS
GTAACATTTGCTTCTTCATT+TGG	0.284164	6.3:+30671500	None:intergenic
GTACTAAGATCTGATCTTCT+TGG	0.327969	6.3:+30670676	None:intergenic
GGAACTTTCTTCATATGTCT+AGG	0.392353	6.3:-30671416	MS.gene75321:CDS
GAACTTTCTTCATATGTCTA+GGG	0.429731	6.3:-30671415	MS.gene75321:CDS
ATTAGTACTGGAAGAGGTGG+AGG	0.434163	6.3:-30670625	MS.gene75321:CDS
TCAAGATTCTCTTCTGCATC+AGG	0.434480	6.3:+30671383	None:intergenic
GATGCAGAAGAGAATCTTGA+TGG	0.462814	6.3:-30671380	MS.gene75321:CDS
AGAAATTCTGAATTAATCCC+TGG	0.466843	6.3:+30670574	None:intergenic
CTTAGTATTGGAAGAGGTCC+AGG	0.467546	6.3:-30670592	MS.gene75321:CDS
GGAAGAGGTGGAGGAAGATC+AGG	0.471350	6.3:-30670616	MS.gene75321:CDS
AGAAGATCAGATATTAGTAC+TGG	0.492577	6.3:-30670637	MS.gene75321:CDS
GGAAGATCAGGTCTTAGTAT+TGG	0.498505	6.3:-30670604	MS.gene75321:CDS
AGAAGATCAGATCTTAGTAC+TGG	0.511957	6.3:-30670673	MS.gene75321:CDS
TTTGGAGTAAAGAACATGGA+TGG	0.540921	6.3:+30671447	None:intergenic
TCAGATCTTAGTACTGGAAG+AGG	0.548826	6.3:-30670667	MS.gene75321:CDS
TCAGATATTAGTACTGGAAG+AGG	0.551309	6.3:-30670631	MS.gene75321:CDS
GAATGTTCAACGAGTGATGA+TGG	0.575026	6.3:-30671338	MS.gene75321:CDS
GACATATGAAGAAAGTTCCA+AGG	0.581756	6.3:+30671420	None:intergenic
TCAGGTCTTAGTATTGGAAG+AGG	0.585484	6.3:-30670598	MS.gene75321:CDS
GATATTAGTACTGGAAGAGG+TGG	0.589476	6.3:-30670628	MS.gene75321:CDS
AAGCAAATGTTACAAATGAG+AGG	0.594342	6.3:-30671490	MS.gene75321:CDS
GATCTTAGTACTGGAAGAGG+TGG	0.621063	6.3:-30670664	MS.gene75321:CDS
TTAGTATTGGAAGAGGTCCA+GGG	0.652209	6.3:-30670591	MS.gene75321:CDS
ACTTTGATAATTAGGAGTGG+CGG	0.657081	6.3:-30670700	MS.gene75321:CDS
AGCACTTTGATAATTAGGAG+TGG	0.663797	6.3:-30670703	MS.gene75321:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGATTAAAAAAAAAAAATTC+GGG	-	chr6.3:30670838-30670857	MS.gene75321:intron	10.0%
!!!	ACTAGTTTTTTTTTTTTTTT+AGG	+	chr6.3:30671008-30671027	None:intergenic	10.0%
!!!	CTAGTTTTTTTTTTTTTTTA+GGG	+	chr6.3:30671007-30671026	None:intergenic	10.0%
!!	AATACTAAATGTTAGTATGT+TGG	-	chr6.3:30671061-30671080	MS.gene75321:intron	20.0%
!!!	TTCAGATTAATTTGTTAATG+AGG	-	chr6.3:30671119-30671138	MS.gene75321:intron	20.0%
!!!	TTTGTTAATGAGGTTTTTTT+TGG	-	chr6.3:30671129-30671148	MS.gene75321:intron	20.0%
!	ACAAATTAATCTGAATGACT+TGG	+	chr6.3:30671114-30671133	None:intergenic	25.0%
!	AGGTTAAACACAAATCATTT+AGG	-	chr6.3:30671256-30671275	MS.gene75321:intron	25.0%
!!	ATATTCTAGAGAAAGATTGT+TGG	+	chr6.3:30671306-30671325	None:intergenic	25.0%
!!	GAAAATCCAAAATCGAATTT+TGG	-	chr6.3:30671278-30671297	MS.gene75321:intron	25.0%
!!	TTATCAGCACTTTGATAATT+AGG	-	chr6.3:30671377-30671396	MS.gene75321:CDS	25.0%
!!!	AATTTTTGGAGTAAAGAACA+TGG	+	chr6.3:30670645-30670664	None:intergenic	25.0%
	AAGCAAATGTTACAAATGAG+AGG	-	chr6.3:30670595-30670614	MS.gene75321:CDS	30.0%
	AGAAATTCTGAATTAATCCC+TGG	+	chr6.3:30671514-30671533	None:intergenic	30.0%
	AGAAGATCAGATATTAGTAC+TGG	-	chr6.3:30671448-30671467	MS.gene75321:CDS	30.0%
	CTCATTAACAATTGTACCTT+CGG	+	chr6.3:30671238-30671257	None:intergenic	30.0%
	GAACTTTCTTCATATGTCTA+GGG	-	chr6.3:30670670-30670689	MS.gene75321:CDS	30.0%
	GTAACATTTGCTTCTTCATT+TGG	+	chr6.3:30670588-30670607	None:intergenic	30.0%
	TCATTAACAATTGTACCTTC+GGG	+	chr6.3:30671237-30671256	None:intergenic	30.0%
	TCTTTACTCCAAAAATTCCT+TGG	-	chr6.3:30670648-30670667	MS.gene75321:CDS	30.0%
	TGGATGGTAAAGACAAAAAT+TGG	+	chr6.3:30670625-30670644	None:intergenic	30.0%
!	AGAAAGTTCCAAGGAATTTT+TGG	+	chr6.3:30670659-30670678	None:intergenic	30.0%
!	ATGTTAGTATGTTGGTGAAT+AGG	-	chr6.3:30671069-30671088	MS.gene75321:intron	30.0%
!	TTGGAACCAAAATTCGATTT+TGG	+	chr6.3:30671287-30671306	None:intergenic	30.0%
!	TTGTGACGTTCCAATTTTAT+CGG	-	chr6.3:30671206-30671225	MS.gene75321:intron	30.0%
	AGAAGATCAGATCTTAGTAC+TGG	-	chr6.3:30671412-30671431	MS.gene75321:CDS	35.0%
	CATTAACAATTGTACCTTCG+GGG	+	chr6.3:30671236-30671255	None:intergenic	35.0%
	CGTCACAAAAAAGATTAGCA+TGG	+	chr6.3:30671194-30671213	None:intergenic	35.0%
	GACATATGAAGAAAGTTCCA+AGG	+	chr6.3:30670668-30670687	None:intergenic	35.0%
	GGAACTTTCTTCATATGTCT+AGG	-	chr6.3:30670669-30670688	MS.gene75321:CDS	35.0%
	GTACTAAGATCTGATCTTCT+TGG	+	chr6.3:30671412-30671431	None:intergenic	35.0%
!	ACTTTGATAATTAGGAGTGG+CGG	-	chr6.3:30671385-30671404	MS.gene75321:CDS	35.0%
!	AGCACTTTGATAATTAGGAG+TGG	-	chr6.3:30671382-30671401	MS.gene75321:CDS	35.0%
!	CGAAGGTACAATTGTTAATG+AGG	-	chr6.3:30671236-30671255	MS.gene75321:intron	35.0%
!	TCAGATATTAGTACTGGAAG+AGG	-	chr6.3:30671454-30671473	MS.gene75321:CDS	35.0%
!	TTAGTATGTTGGTGAATAGG+TGG	-	chr6.3:30671072-30671091	MS.gene75321:intron	35.0%
!	TTTGGAGTAAAGAACATGGA+TGG	+	chr6.3:30670641-30670660	None:intergenic	35.0%
!!	CTGAATGACTTGGTTCTATA+AGG	+	chr6.3:30671104-30671123	None:intergenic	35.0%
	GAATGTTCAACGAGTGATGA+TGG	-	chr6.3:30670747-30670766	MS.gene75321:intron	40.0%
	GATGCAGAAGAGAATCTTGA+TGG	-	chr6.3:30670705-30670724	MS.gene75321:CDS	40.0%
	GGAAGATCAGGTCTTAGTAT+TGG	-	chr6.3:30671481-30671500	MS.gene75321:CDS	40.0%
	TCAAGATTCTCTTCTGCATC+AGG	+	chr6.3:30670705-30670724	None:intergenic	40.0%
!	GATATTAGTACTGGAAGAGG+TGG	-	chr6.3:30671457-30671476	MS.gene75321:CDS	40.0%
!	TCAGATCTTAGTACTGGAAG+AGG	-	chr6.3:30671418-30671437	MS.gene75321:CDS	40.0%
!	TCAGGTCTTAGTATTGGAAG+AGG	-	chr6.3:30671487-30671506	MS.gene75321:CDS	40.0%
!	TTAGTATTGGAAGAGGTCCA+GGG	-	chr6.3:30671494-30671513	MS.gene75321:CDS	40.0%
	ATTAGCATGGCTCCTAGACA+AGG	+	chr6.3:30671181-30671200	None:intergenic	45.0%
	TCGGGGACATCCGATAAAAT+TGG	+	chr6.3:30671219-30671238	None:intergenic	45.0%
	TGTGCATGTCATCCTTGTCT+AGG	-	chr6.3:30671166-30671185	MS.gene75321:intron	45.0%
!	ATTAGTACTGGAAGAGGTGG+AGG	-	chr6.3:30671460-30671479	MS.gene75321:CDS	45.0%
!	ATTTTATCGGATGTCCCCGA+AGG	-	chr6.3:30671219-30671238	MS.gene75321:intron	45.0%
!	CTTAGTATTGGAAGAGGTCC+AGG	-	chr6.3:30671493-30671512	MS.gene75321:CDS	45.0%
!	GATCTTAGTACTGGAAGAGG+TGG	-	chr6.3:30671421-30671440	MS.gene75321:CDS	45.0%
!!	ATGATTAAAAAAAAAAAATT+CGG	-	chr6.3:30670837-30670856	MS.gene75321:intron	5.0%
	GGAAGAGGTGGAGGAAGATC+AGG	-	chr6.3:30671469-30671488	MS.gene75321:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.3	gene	30670570	30671537	30670570	ID=MS.gene75321
chr6.3	mRNA	30670570	30671537	30670570	ID=MS.gene75321.t1;Parent=MS.gene75321
chr6.3	exon	30671328	30671537	30671328	ID=MS.gene75321.t1.exon1;Parent=MS.gene75321.t1
chr6.3	CDS	30671328	30671537	30671328	ID=cds.MS.gene75321.t1;Parent=MS.gene75321.t1
chr6.3	exon	30670570	30670734	30670570	ID=MS.gene75321.t1.exon2;Parent=MS.gene75321.t1
chr6.3	CDS	30670570	30670734	30670570	ID=cds.MS.gene75321.t1;Parent=MS.gene75321.t1

Gene Sequence

>MS.gene75321

ATGGAAGAAATATCACCAAATGAAGAAGCAAATGTTACAAATGAGAGGAGTGCCAATTTTTGTCTTTACCATCCATGTTCTTTACTCCAAAAATTCCTTGGAACTTTCTTCATATGTCTAGGGTTTCAAATTCCTGATGCAGAAGAGAATCTTGATGGCAAAGTTCTTGCATGTGAAGAATGTTCAACGAGTGATGATGGTCAAAGTTTTGTAAGCTTTTCTCTCTTTTTAATTTTTTTTGACTGAATTCTTTCTTTTTAATTTGAGATGATTAAAAAAAAAAAATTCGGGTATTCATTTGTTTCTTATTTCTTCAATGAGAATCATCTTGTAAGTAAAAAAAATGATTAATTTGACAAAACTAAAATCAATTAAAAAAAAATAAAAAATAAGAGTTGATATTTTTAACACATCTATAATTAGTTTAGTTATTTCCCTAAAAAAAAAAAAAAACTAGTTATTATGACTATTTTGAACAACTAAAACTTGTTAATACTAAATGTTAGTATGTTGGTGAATAGGTGGAATCAACCTTATAGAACCAAGTCATTCAGATTAATTTGTTAATGAGGTTTTTTTTGGACAATTTCTTGATTTGTGCATGTCATCCTTGTCTAGGAGCCATGCTAATCTTTTTTGTGACGTTCCAATTTTATCGGATGTCCCCGAAGGTACAATTGTTAATGAGGTTAAACACAAATCATTTAGGAAAATCCAAAATCGAATTTTGGTTCCAACAATCTTTCTCTAGAATATAAGCAAAGTATTCACACATTCTGATTTGTATTTTCTTTTTCTTACAGCAACTTATCAGCACTTTGATAATTAGGAGTGGCGGACCAAGAAGATCAGATCTTAGTACTGGAAGAGGTGGACGAAGAAGATCAGATATTAGTACTGGAAGAGGTGGAGGAAGATCAGGTCTTAGTATTGGAAGAGGTCCAGGGATTAATTCAGAATTTCTCTAA

Protein sequence

>MS.gene75321.t1

MEEISPNEEANVTNERSANFCLYHPCSLLQKFLGTFFICLGFQIPDAEENLDGKVLACEECSTSDDGQSFQLISTLIIRSGGPRRSDLSTGRGGRRRSDISTGRGGGRSGLSIGRGPGINSEFL