AlfalfaGEDB | MS.gene35695

M. sativa cultivar XinJiangDaYe / MS.gene35695

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35695.t1	RHN75471.1	98.5	130	2	0	3	132	80	209	2.20E-68	268.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35695.t1	G7IHF3	98.5	130	2	0	3	132	43	172	1.6e-68	268.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049260	MS.gene35695	0.801475	8.85E-49	-1.69E-46
MS.gene049382	MS.gene35695	0.821906	3.15E-53	-1.69E-46
MS.gene050020	MS.gene35695	0.865932	4.13E-65	-1.69E-46
MS.gene050128	MS.gene35695	0.805093	1.58E-49	-1.69E-46
MS.gene050206	MS.gene35695	0.809912	1.50E-50	-1.69E-46
MS.gene050584	MS.gene35695	0.922292	1.19E-88	-1.69E-46
MS.gene050585	MS.gene35695	0.891189	4.98E-74	-1.69E-46
MS.gene050586	MS.gene35695	0.881234	2.84E-70	-1.69E-46
MS.gene050587	MS.gene35695	0.86871	5.33E-66	-1.69E-46
MS.gene050762	MS.gene35695	0.808026	3.79E-50	-1.69E-46
MS.gene050763	MS.gene35695	0.808452	3.08E-50	-1.69E-46
MS.gene050807	MS.gene35695	0.834582	2.76E-56	-1.69E-46
MS.gene050808	MS.gene35695	0.806982	6.31E-50	-1.69E-46
MS.gene050815	MS.gene35695	0.805808	1.12E-49	-1.69E-46
MS.gene05143	MS.gene35695	0.824979	6.02E-54	-1.69E-46
MS.gene051626	MS.gene35695	0.872413	3.24E-67	-1.69E-46
MS.gene051789	MS.gene35695	0.917083	8.17E-86	-1.69E-46
MS.gene052142	MS.gene35695	0.851505	8.50E-61	-1.69E-46
MS.gene052187	MS.gene35695	0.817311	3.52E-52	-1.69E-46
MS.gene052665	MS.gene35695	0.827202	1.78E-54	-1.69E-46
MS.gene052698	MS.gene35695	0.811663	6.26E-51	-1.69E-46
MS.gene052967	MS.gene35695	0.868153	8.07E-66	-1.69E-46
MS.gene053074	MS.gene35695	0.934528	3.51E-96	-1.69E-46
MS.gene053204	MS.gene35695	0.854107	1.53E-61	-1.69E-46
MS.gene053383	MS.gene35695	0.826561	2.54E-54	-1.69E-46
MS.gene053563	MS.gene35695	0.807313	5.37E-50	-1.69E-46
MS.gene053630	MS.gene35695	0.815957	7.08E-52	-1.69E-46
MS.gene054184	MS.gene35695	0.863396	2.56E-64	-1.69E-46
MS.gene054185	MS.gene35695	0.865709	4.85E-65	-1.69E-46
MS.gene054214	MS.gene35695	0.817819	2.71E-52	-1.69E-46
MS.gene054379	MS.gene35695	0.805677	1.19E-49	-1.69E-46
MS.gene05456	MS.gene35695	0.902012	1.50E-78	-1.69E-46
MS.gene054596	MS.gene35695	0.882421	1.06E-70	-1.69E-46
MS.gene055085	MS.gene35695	0.808153	3.56E-50	-1.69E-46
MS.gene055133	MS.gene35695	0.831423	1.68E-55	-1.69E-46
MS.gene055207	MS.gene35695	0.861863	7.61E-64	-1.69E-46
MS.gene055359	MS.gene35695	0.873716	1.18E-67	-1.69E-46
MS.gene055604	MS.gene35695	0.808278	3.35E-50	-1.69E-46
MS.gene056069	MS.gene35695	-0.807067	6.06E-50	-1.69E-46
MS.gene056760	MS.gene35695	0.814204	1.73E-51	-1.69E-46
MS.gene057750	MS.gene35695	0.806973	6.34E-50	-1.69E-46
MS.gene057754	MS.gene35695	0.845178	4.78E-59	-1.69E-46
MS.gene058168	MS.gene35695	0.885925	5.34E-72	-1.69E-46
MS.gene058982	MS.gene35695	0.80174	7.81E-49	-1.69E-46
MS.gene059006	MS.gene35695	0.924398	7.43E-90	-1.69E-46
MS.gene059124	MS.gene35695	0.901423	2.72E-78	-1.69E-46
MS.gene060320	MS.gene35695	0.815282	1.00E-51	-1.69E-46
MS.gene060470	MS.gene35695	0.800086	1.70E-48	-1.69E-46
MS.gene060476	MS.gene35695	0.82264	2.13E-53	-1.69E-46
MS.gene060513	MS.gene35695	0.80895	2.41E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35695.t1	MTR_2g086680	98.462	130	2	0	3	132	43	172	7.10e-94	268

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35695.t1	AT5G22430	45.926	135	61	5	3	132	45	172	4.64e-27	99.0
MS.gene35695.t1	AT2G27385	43.077	130	63	3	3	132	44	162	7.66e-27	98.2
MS.gene35695.t1	AT2G27385	43.077	130	63	3	3	132	55	173	1.42e-26	97.8
MS.gene35695.t1	AT2G27385	43.077	130	63	3	3	132	55	173	1.42e-26	97.8

Find 34 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TACAACTCTACCATGGTTTC+AGG	0.202226	2.4:-13666348	None:intergenic
CCTTCACAATTTGGGAGAAT+TGG	0.256434	2.4:+13666154	None:intergenic
TACCATGGTTTCAGGAATTA+AGG	0.295653	2.4:-13666340	None:intergenic
CTTCCTTTCTTCCCCATCAT+TGG	0.318190	2.4:-13665970	MS.gene35695:CDS
CTTGCCTCCAGAGTGGGGTT+TGG	0.331819	2.4:-13666010	MS.gene35695:CDS
AATTGCCATGCAAAACTTCT+TGG	0.358597	2.4:-13666210	MS.gene35695:CDS
GGTTTCTTCTTGAAGCATAA+AGG	0.387581	2.4:+13666175	None:intergenic
CTTGAAGCATAAAGGTTAGT+TGG	0.400325	2.4:+13666183	None:intergenic
TTTCCCCTTGCCTCCAGAGT+GGG	0.435282	2.4:-13666016	MS.gene35695:CDS
TTTGCATGGCAATTATCATA+AGG	0.435517	2.4:+13666219	None:intergenic
AGAAAATGATGGCTCCTTCA+TGG	0.464326	2.4:-13666271	MS.gene35695:CDS
TGATCATGGTATTCCAATGA+TGG	0.466506	2.4:+13665957	None:intergenic
AAACCCCACTCTGGAGGCAA+GGG	0.479581	2.4:+13666012	None:intergenic
CCAAATTGTGAAGGGAAAAG+AGG	0.515617	2.4:-13666145	MS.gene35695:CDS
ATTCCAATGATGGGGAAGAA+AGG	0.527851	2.4:+13665967	None:intergenic
TGCCATGCAAAACTTCTTGG+TGG	0.529749	2.4:-13666207	MS.gene35695:CDS
CTTGGTGCCAAACCCCACTC+TGG	0.534566	2.4:+13666003	None:intergenic
CAAACCCCACTCTGGAGGCA+AGG	0.536034	2.4:+13666011	None:intergenic
AGTGAAGAAACTGAGAGGAG+TGG	0.538559	2.4:+13666104	None:intergenic
TTCCCCTTGCCTCCAGAGTG+GGG	0.538731	2.4:-13666015	MS.gene35695:CDS
GATCATGGTATTCCAATGAT+GGG	0.544352	2.4:+13665958	None:intergenic
GACCTTAATTCCTGAAACCA+TGG	0.548781	2.4:+13666338	None:intergenic
CCAATTCTCCCAAATTGTGA+AGG	0.561971	2.4:-13666154	MS.gene35695:CDS
GCCATGCAAAACTTCTTGGT+GGG	0.568791	2.4:-13666206	MS.gene35695:CDS
ATTTCCCCTTGCCTCCAGAG+TGG	0.574643	2.4:-13666017	MS.gene35695:CDS
AAAGGAAGATAATAACTACT+TGG	0.580003	2.4:+13665985	None:intergenic
AACCCCACTCTGGAGGCAAG+GGG	0.597617	2.4:+13666013	None:intergenic
ATGGCAACTACAGAAAATGA+TGG	0.598455	2.4:-13666282	MS.gene35695:CDS
AAGGTCTCAATGAAGTGTGA+AGG	0.600342	2.4:-13666321	MS.gene35695:CDS
CAATTCTCCCAAATTGTGAA+GGG	0.607626	2.4:-13666153	MS.gene35695:CDS
TGTGGAAAGATCAACCATGA+AGG	0.637832	2.4:+13666257	None:intergenic
GGTGCCAAACCCCACTCTGG+AGG	0.646400	2.4:+13666006	None:intergenic
ATCATGGTATTCCAATGATG+GGG	0.661584	2.4:+13665959	None:intergenic
CAAGAAGTGAAGAAACTGAG+AGG	0.684855	2.4:+13666099	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAACTGAGAAAAATGAGTT+TGG	-	chr2.4:13666239-13666258	MS.gene35695:CDS	25.0%
!	AAAGGAAGATAATAACTACT+TGG	+	chr2.4:13666314-13666333	None:intergenic	25.0%
!!!	AGTTTTGCATTCTTTGTTTT+CGG	+	chr2.4:13666225-13666244	None:intergenic	25.0%
	TTTGCATGGCAATTATCATA+AGG	+	chr2.4:13666080-13666099	None:intergenic	30.0%
!	TATCATAAGGAAGTTTTGCA+TGG	+	chr2.4:13666067-13666086	None:intergenic	30.0%
!!!	GTTTTGCATTCTTTGTTTTC+GGG	+	chr2.4:13666224-13666243	None:intergenic	30.0%
!!!	TTTTGCATTCTTTGTTTTCG+GGG	+	chr2.4:13666223-13666242	None:intergenic	30.0%
	AATTGCCATGCAAAACTTCT+TGG	-	chr2.4:13666086-13666105	MS.gene35695:CDS	35.0%
	ATGGCAACTACAGAAAATGA+TGG	-	chr2.4:13666014-13666033	MS.gene35695:CDS	35.0%
	CAATTCTCCCAAATTGTGAA+GGG	-	chr2.4:13666143-13666162	MS.gene35695:CDS	35.0%
	CTTGAAGCATAAAGGTTAGT+TGG	+	chr2.4:13666116-13666135	None:intergenic	35.0%
	GGTTTCTTCTTGAAGCATAA+AGG	+	chr2.4:13666124-13666143	None:intergenic	35.0%
!	CTCTTTTCCCTTCACAATTT+GGG	+	chr2.4:13666153-13666172	None:intergenic	35.0%
	AAGGTCTCAATGAAGTGTGA+AGG	-	chr2.4:13665975-13665994	MS.gene35695:CDS	40.0%
	AGAAAATGATGGCTCCTTCA+TGG	-	chr2.4:13666025-13666044	MS.gene35695:CDS	40.0%
	AGGAGTGAAAAACATGGCAA+TGG	-	chr2.4:13665995-13666014	MS.gene35695:CDS	40.0%
	CAAGAAGTGAAGAAACTGAG+AGG	+	chr2.4:13666200-13666219	None:intergenic	40.0%
	CCAAATTGTGAAGGGAAAAG+AGG	-	chr2.4:13666151-13666170	MS.gene35695:CDS	40.0%
	CCAATTCTCCCAAATTGTGA+AGG	-	chr2.4:13666142-13666161	MS.gene35695:CDS	40.0%
	CCTTCACAATTTGGGAGAAT+TGG	+	chr2.4:13666145-13666164	None:intergenic	40.0%
	GTGTGAAGGAGTGAAAAACA+TGG	-	chr2.4:13665989-13666008	MS.gene35695:CDS	40.0%
	TGTGGAAAGATCAACCATGA+AGG	+	chr2.4:13666042-13666061	None:intergenic	40.0%
!	ATTCCAATGATGGGGAAGAA+AGG	+	chr2.4:13666332-13666351	None:intergenic	40.0%
!	CCTCTTTTCCCTTCACAATT+TGG	+	chr2.4:13666154-13666173	None:intergenic	40.0%
	AGTGAAGAAACTGAGAGGAG+TGG	+	chr2.4:13666195-13666214	None:intergenic	45.0%
	CTTCCTTTCTTCCCCATCAT+TGG	-	chr2.4:13666326-13666345	MS.gene35695:CDS	45.0%
	GCCATGCAAAACTTCTTGGT+GGG	-	chr2.4:13666090-13666109	MS.gene35695:CDS	45.0%
	TGCCATGCAAAACTTCTTGG+TGG	-	chr2.4:13666089-13666108	MS.gene35695:CDS	45.0%
!	AGGAAGTTTTGCATGGCTTG+TGG	+	chr2.4:13666060-13666079	None:intergenic	45.0%
!	GCCCACCAAGAAGTTTTGCA+TGG	+	chr2.4:13666094-13666113	None:intergenic	50.0%
	ATTTCCCCTTGCCTCCAGAG+TGG	-	chr2.4:13666279-13666298	MS.gene35695:CDS	55.0%
	TTTCCCCTTGCCTCCAGAGT+GGG	-	chr2.4:13666280-13666299	MS.gene35695:CDS	55.0%
!	AAACCCCACTCTGGAGGCAA+GGG	+	chr2.4:13666287-13666306	None:intergenic	55.0%
	CTTGCCTCCAGAGTGGGGTT+TGG	-	chr2.4:13666286-13666305	MS.gene35695:CDS	60.0%
	TTCCCCTTGCCTCCAGAGTG+GGG	-	chr2.4:13666281-13666300	MS.gene35695:CDS	60.0%
!	AACCCCACTCTGGAGGCAAG+GGG	+	chr2.4:13666286-13666305	None:intergenic	60.0%
!	CAAACCCCACTCTGGAGGCA+AGG	+	chr2.4:13666288-13666307	None:intergenic	60.0%
!!	CTTGGTGCCAAACCCCACTC+TGG	+	chr2.4:13666296-13666315	None:intergenic	60.0%
	GGTGCCAAACCCCACTCTGG+AGG	+	chr2.4:13666293-13666312	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	13665960	13666358	13665960	ID=MS.gene35695
chr2.4	mRNA	13665960	13666358	13665960	ID=MS.gene35695.t1;Parent=MS.gene35695
chr2.4	exon	13665960	13666358	13665960	ID=MS.gene35695.t1.exon1;Parent=MS.gene35695.t1
chr2.4	CDS	13665960	13666358	13665960	ID=cds.MS.gene35695.t1;Parent=MS.gene35695.t1

Gene Sequence

>MS.gene35695

ATGGTTTCAGGAATTAAGGTCTCAATGAAGTGTGAAGGAGTGAAAAACATGGCAATGGCAACTACAGAAAATGATGGCTCCTTCATGGTTGATCTTTCCACAAGCCATGCAAAACTTCCTTATGATAATTGCCATGCAAAACTTCTTGGTGGGCCAACTAACCTTTATGCTTCAAGAAGAAACCAATTCTCCCAAATTGTGAAGGGAAAAGAGGAAAATAGCTACACACTATCCACTCCTCTCAGTTTCTTCACTTCTTGCCCCGAAAACAAAGAATGCAAAACTGAGAAAAATGAGTTTGGTTCATCAAAAAGCATCAATTTCCCCTTGCCTCCAGAGTGGGGTTTGGCACCAAGTAGTTATTATCTTCCTTTCTTCCCCATCATTGGAATACCATGA

Protein sequence

>MS.gene35695.t1

MVSGIKVSMKCEGVKNMAMATTENDGSFMVDLSTSHAKLPYDNCHAKLLGGPTNLYASRRNQFSQIVKGKEENSYTLSTPLSFFTSCPENKECKTEKNEFGSSKSINFPLPPEWGLAPSSYYLPFFPIIGIP