Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88718.t1 | XP_024639822.1 | 99.5 | 566 | 3 | 0 | 1 | 566 | 1 | 566 | 0.00E+00 | 1164.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88718.t1 | G7KE15 | 99.5 | 566 | 3 | 0 | 1 | 566 | 1 | 566 | 0.0e+00 | 1164.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049807 | MS.gene88718 | 0.858089 | 1.05E-62 | -1.69E-46 |
| MS.gene049887 | MS.gene88718 | 0.892769 | 1.17E-74 | -1.69E-46 |
| MS.gene050551 | MS.gene88718 | 0.823787 | 1.15E-53 | -1.69E-46 |
| MS.gene050689 | MS.gene88718 | 0.839523 | 1.51E-57 | -1.69E-46 |
| MS.gene050838 | MS.gene88718 | 0.878833 | 2.04E-69 | -1.69E-46 |
| MS.gene051102 | MS.gene88718 | 0.825981 | 3.48E-54 | -1.69E-46 |
| MS.gene051234 | MS.gene88718 | 0.85231 | 5.02E-61 | -1.69E-46 |
| MS.gene051642 | MS.gene88718 | 0.817517 | 3.16E-52 | -1.69E-46 |
| MS.gene051687 | MS.gene88718 | 0.805885 | 1.08E-49 | -1.69E-46 |
| MS.gene051850 | MS.gene88718 | 0.84418 | 8.87E-59 | -1.69E-46 |
| MS.gene051885 | MS.gene88718 | 0.888664 | 4.83E-73 | -1.69E-46 |
| MS.gene052133 | MS.gene88718 | 0.84041 | 8.84E-58 | -1.69E-46 |
| MS.gene05224 | MS.gene88718 | 0.807667 | 4.52E-50 | -1.69E-46 |
| MS.gene052307 | MS.gene88718 | 0.807018 | 6.20E-50 | -1.69E-46 |
| MS.gene052335 | MS.gene88718 | 0.878903 | 1.93E-69 | -1.69E-46 |
| MS.gene052397 | MS.gene88718 | 0.831875 | 1.30E-55 | -1.69E-46 |
| MS.gene052514 | MS.gene88718 | 0.829216 | 5.83E-55 | -1.69E-46 |
| MS.gene052516 | MS.gene88718 | 0.838889 | 2.20E-57 | -1.69E-46 |
| MS.gene052609 | MS.gene88718 | 0.818806 | 1.62E-52 | -1.69E-46 |
| MS.gene052732 | MS.gene88718 | 0.862208 | 5.96E-64 | -1.69E-46 |
| MS.gene053143 | MS.gene88718 | 0.846414 | 2.20E-59 | -1.69E-46 |
| MS.gene053144 | MS.gene88718 | 0.889512 | 2.27E-73 | -1.69E-46 |
| MS.gene053171 | MS.gene88718 | 0.808504 | 3.00E-50 | -1.69E-46 |
| MS.gene053174 | MS.gene88718 | 0.808295 | 3.32E-50 | -1.69E-46 |
| MS.gene053581 | MS.gene88718 | 0.884688 | 1.55E-71 | -1.69E-46 |
| MS.gene054250 | MS.gene88718 | 0.813256 | 2.81E-51 | -1.69E-46 |
| MS.gene054550 | MS.gene88718 | 0.807763 | 4.31E-50 | -1.69E-46 |
| MS.gene054554 | MS.gene88718 | 0.813913 | 2.01E-51 | -1.69E-46 |
| MS.gene054584 | MS.gene88718 | 0.879651 | 1.05E-69 | -1.69E-46 |
| MS.gene054824 | MS.gene88718 | 0.806806 | 6.88E-50 | -1.69E-46 |
| MS.gene055221 | MS.gene88718 | 0.803364 | 3.61E-49 | -1.69E-46 |
| MS.gene055465 | MS.gene88718 | 0.833857 | 4.19E-56 | -1.69E-46 |
| MS.gene055466 | MS.gene88718 | 0.819088 | 1.40E-52 | -1.69E-46 |
| MS.gene055468 | MS.gene88718 | 0.846689 | 1.86E-59 | -1.69E-46 |
| MS.gene055469 | MS.gene88718 | 0.856062 | 4.15E-62 | -1.69E-46 |
| MS.gene055537 | MS.gene88718 | 0.826343 | 2.86E-54 | -1.69E-46 |
| MS.gene055580 | MS.gene88718 | -0.866891 | 2.05E-65 | -1.69E-46 |
| MS.gene055890 | MS.gene88718 | 0.802609 | 5.17E-49 | -1.69E-46 |
| MS.gene055891 | MS.gene88718 | 0.855832 | 4.84E-62 | -1.69E-46 |
| MS.gene055979 | MS.gene88718 | 0.812163 | 4.87E-51 | -1.69E-46 |
| MS.gene056043 | MS.gene88718 | -0.873282 | 1.66E-67 | -1.69E-46 |
| MS.gene056075 | MS.gene88718 | 0.838146 | 3.42E-57 | -1.69E-46 |
| MS.gene056308 | MS.gene88718 | 0.812686 | 3.74E-51 | -1.69E-46 |
| MS.gene056396 | MS.gene88718 | 0.809124 | 2.21E-50 | -1.69E-46 |
| MS.gene056432 | MS.gene88718 | 0.801182 | 1.02E-48 | -1.69E-46 |
| MS.gene058149 | MS.gene88718 | 0.813256 | 2.81E-51 | -1.69E-46 |
| MS.gene058668 | MS.gene88718 | 0.8012 | 1.01E-48 | -1.69E-46 |
| MS.gene058720 | MS.gene88718 | 0.820496 | 6.65E-53 | -1.69E-46 |
| MS.gene058852 | MS.gene88718 | 0.822938 | 1.81E-53 | -1.69E-46 |
| MS.gene058945 | MS.gene88718 | 0.815015 | 1.15E-51 | -1.69E-46 |
| MS.gene059171 | MS.gene88718 | 0.834487 | 2.91E-56 | -1.69E-46 |
| MS.gene059227 | MS.gene88718 | 0.806981 | 6.32E-50 | -1.69E-46 |
| MS.gene060194 | MS.gene88718 | 0.841702 | 4.05E-58 | -1.69E-46 |
| MS.gene060587 | MS.gene88718 | 0.845512 | 3.88E-59 | -1.69E-46 |
| MS.gene060884 | MS.gene88718 | 0.812019 | 5.23E-51 | -1.69E-46 |
| MS.gene060886 | MS.gene88718 | 0.810077 | 1.38E-50 | -1.69E-46 |
| MS.gene061006 | MS.gene88718 | 0.843177 | 1.64E-58 | -1.69E-46 |
| MS.gene061100 | MS.gene88718 | 0.807775 | 4.29E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene09087 | MS.gene88718 | PPI |
| MS.gene88718 | MS.gene023532 | PPI |
| MS.gene88718 | MS.gene08769 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88718.t1 | MTR_5g069410 | 99.470 | 566 | 3 | 0 | 1 | 566 | 1 | 566 | 0.0 | 1180 |
| MS.gene88718.t1 | MTR_8g085780 | 48.325 | 567 | 265 | 6 | 14 | 561 | 16 | 573 | 0.0 | 565 |
| MS.gene88718.t1 | MTR_8g106395 | 23.948 | 309 | 190 | 9 | 271 | 553 | 75 | 364 | 7.01e-16 | 80.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88718.t1 | AT4G12700 | 74.427 | 567 | 134 | 5 | 1 | 564 | 1 | 559 | 0.0 | 895 |
| MS.gene88718.t1 | AT2G04280 | 74.868 | 569 | 135 | 6 | 1 | 564 | 1 | 566 | 0.0 | 895 |
| MS.gene88718.t1 | AT4G08810 | 47.965 | 565 | 254 | 5 | 16 | 561 | 9 | 552 | 0.0 | 521 |
| MS.gene88718.t1 | AT3G56750 | 23.473 | 311 | 188 | 10 | 271 | 553 | 87 | 375 | 3.47e-16 | 81.3 |
| MS.gene88718.t1 | AT2G41150 | 22.581 | 310 | 193 | 10 | 271 | 553 | 87 | 376 | 4.00e-16 | 81.3 |
Find 126 sgRNAs with CRISPR-Local
Find 152 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACGTTTAGTGTTGTTAGTA+TGG | 0.260774 | 5.4:+54205305 | MS.gene88718:CDS |
| CAATAGCTTCAAGGTTAAAC+TGG | 0.270199 | 5.4:+54205976 | MS.gene88718:CDS |
| GGTTTCAGACCAGAGAATTT+AGG | 0.279478 | 5.4:+54204751 | MS.gene88718:CDS |
| TTCATCTGGTCGAGGATTAC+AGG | 0.297935 | 5.4:+54205781 | MS.gene88718:CDS |
| GCTTTAATCATGAGAAAGTT+TGG | 0.298138 | 5.4:+54205837 | MS.gene88718:CDS |
| TTTATGTGGGTGGTGGAGAT+AGG | 0.323850 | 5.4:+54205505 | MS.gene88718:CDS |
| AAAGGCCATGGCATTTGATA+TGG | 0.327645 | 5.4:+54205922 | MS.gene88718:CDS |
| TAAATTTGGGAAACCTGTTC+CGG | 0.333058 | 5.4:+54205170 | MS.gene88718:CDS |
| ACTCAGTGTGAGGGGAATTC+AGG | 0.333650 | 5.4:+54205066 | MS.gene88718:CDS |
| CCATAACCAATGTTCGGTTT+AGG | 0.341807 | 5.4:-54205587 | None:intergenic |
| AGTGTTTGTTATTGGTGTTT+TGG | 0.342766 | 5.4:+54204813 | MS.gene88718:CDS |
| ACTCTATACCAATAGTTATC+AGG | 0.346554 | 5.4:-54205870 | None:intergenic |
| GGATGAAACAAAGGATCTTC+AGG | 0.354420 | 5.4:-54204865 | None:intergenic |
| TTCACATCCATAAGTTTCAT+TGG | 0.367179 | 5.4:-54205810 | None:intergenic |
| AGAGAAGACATCGTCGAATC+TGG | 0.368044 | 5.4:+54205386 | MS.gene88718:CDS |
| GGTCACACCAATGAAACTTA+TGG | 0.375350 | 5.4:+54205803 | MS.gene88718:CDS |
| TGTTGGACAAGGATCAGTTT+TGG | 0.376150 | 5.4:+54205718 | MS.gene88718:CDS |
| TCAACGAGTATAAGGAACTT+TGG | 0.387999 | 5.4:+54206204 | MS.gene88718:CDS |
| CCTAAACCGAACATTGGTTA+TGG | 0.388632 | 5.4:+54205587 | MS.gene88718:CDS |
| CAGAATACTATGGCCATGAT+TGG | 0.392276 | 5.4:+54204775 | MS.gene88718:CDS |
| GAGGTATGTGCATCAAGATT+TGG | 0.397669 | 5.4:-54206056 | None:intergenic |
| CAACGAGTATAAGGAACTTT+GGG | 0.400281 | 5.4:+54206205 | MS.gene88718:CDS |
| GTGATATGGCGTGGAGGTAT+AGG | 0.404031 | 5.4:+54205211 | MS.gene88718:CDS |
| TGCACTTCTCTCAACCTTAC+GGG | 0.405105 | 5.4:+54206085 | MS.gene88718:CDS |
| AGGTAAATACTTGGTTTATG+TGG | 0.409434 | 5.4:+54205491 | MS.gene88718:CDS |
| GAATGATAGTACCTGTGATA+TGG | 0.418806 | 5.4:+54205197 | MS.gene88718:CDS |
| GCTACTAATGAGTCTGTGTT+TGG | 0.419365 | 5.4:+54204979 | MS.gene88718:CDS |
| ATGGAAATCGAGGCGATTGA+TGG | 0.422328 | 5.4:+54205941 | MS.gene88718:CDS |
| TGGAGTTTCTTGTGTGCTCT+AGG | 0.424785 | 5.4:+54205552 | MS.gene88718:CDS |
| GGCGTTCCAGTAGAATTTGA+TGG | 0.427805 | 5.4:+54206272 | MS.gene88718:CDS |
| AAATTTGGGAAACCTGTTCC+GGG | 0.431548 | 5.4:+54205171 | MS.gene88718:CDS |
| GATAACAGTGTTGTGAAAAC+TGG | 0.435692 | 5.4:+54204943 | MS.gene88718:CDS |
| ACGTTTAGTGTTGTTAGTAT+GGG | 0.436986 | 5.4:+54205306 | MS.gene88718:CDS |
| TAGTGTCTGCAATAGCTTCA+AGG | 0.440816 | 5.4:+54205967 | MS.gene88718:CDS |
| TAATAGTGATTAAGAATGTT+GGG | 0.441086 | 5.4:+54204703 | None:intergenic |
| GGATCAGGTGAATGCGTTGC+CGG | 0.441220 | 5.4:+54205407 | MS.gene88718:CDS |
| CGTTTAGTGTTGTTAGTATG+GGG | 0.454205 | 5.4:+54205307 | MS.gene88718:CDS |
| AATGTTGTAATCTTAGTTGA+AGG | 0.455380 | 5.4:-54204886 | None:intergenic |
| GTGAATGCGTTGCCGGTTGT+TGG | 0.456460 | 5.4:+54205414 | MS.gene88718:CDS |
| TTCACAAATTCTCCAACAAC+CGG | 0.456505 | 5.4:-54205426 | None:intergenic |
| GTATTCTGACCTAAATTCTC+TGG | 0.456518 | 5.4:-54204760 | None:intergenic |
| ATGTAACCATCAAATTCTAC+TGG | 0.457405 | 5.4:-54206278 | None:intergenic |
| AGATGGGATGAATCTTCATC+TGG | 0.466104 | 5.4:+54205767 | MS.gene88718:CDS |
| GAATTTAGGTCAGAATACTA+TGG | 0.467250 | 5.4:+54204765 | MS.gene88718:CDS |
| GAACTTTCCGACTCAACTAC+AGG | 0.470123 | 5.4:-54205459 | None:intergenic |
| AGGCGAGGAACAAAGAGCTT+TGG | 0.473800 | 5.4:+54206033 | MS.gene88718:CDS |
| TCAGGGTTCTTACAATCAAT+AGG | 0.475567 | 5.4:-54205090 | None:intergenic |
| TCATGTTGTGAGAGGGGAAA+AGG | 0.479362 | 5.4:+54206013 | MS.gene88718:CDS |
| CTATCATTCGAACCCGGAAC+AGG | 0.480882 | 5.4:-54205183 | None:intergenic |
| TTCGTCAACCTTGTCCCGTA+AGG | 0.484068 | 5.4:-54206099 | None:intergenic |
| ATCAGACTTAAAAGTAGCAT+TGG | 0.486545 | 5.4:-54204923 | None:intergenic |
| AAGGTTGAGAGAAGTGCATC+GGG | 0.490128 | 5.4:-54206080 | None:intergenic |
| TTCTCTGGTCTGAAACCTCC+AGG | 0.490679 | 5.4:-54204745 | None:intergenic |
| ATGCACTTCTCTCAACCTTA+CGG | 0.491937 | 5.4:+54206084 | MS.gene88718:CDS |
| GCCGCAGCCTTCCCATCCTT+CGG | 0.493260 | 5.4:-54205234 | None:intergenic |
| ATTTAGTCAAGGTAAATACT+TGG | 0.498121 | 5.4:+54205482 | MS.gene88718:CDS |
| TTTGGGCAGATTGGAGTAAG+TGG | 0.499384 | 5.4:+54205736 | MS.gene88718:CDS |
| TCAAATCCATAACCAATGTT+CGG | 0.500832 | 5.4:-54205593 | None:intergenic |
| GACATCGTCGAATCTGGATC+AGG | 0.504087 | 5.4:+54205392 | MS.gene88718:CDS |
| ATGTCGAGCCTGATAACTAT+TGG | 0.506139 | 5.4:+54205862 | MS.gene88718:CDS |
| AAAACAAACATTCCCAATCA+TGG | 0.507885 | 5.4:-54204788 | None:intergenic |
| AGGAAGAAGAATCAAAAGGC+TGG | 0.511553 | 5.4:+54205360 | MS.gene88718:CDS |
| GGTAAATACTTGGTTTATGT+GGG | 0.513383 | 5.4:+54205492 | MS.gene88718:CDS |
| GGATCAAAGAAAGATGCATC+AGG | 0.513790 | 5.4:-54206152 | None:intergenic |
| AGAATACTATGGCCATGATT+GGG | 0.518270 | 5.4:+54204776 | MS.gene88718:CDS |
| CTGGTATGAATGCGAGGAAG+AGG | 0.518699 | 5.4:+54205340 | MS.gene88718:CDS |
| ATCTTTCTTTGATCCTCTGA+AGG | 0.524319 | 5.4:+54206160 | MS.gene88718:CDS |
| GAAGGAGGCGGCGTCTGTGT+TGG | 0.531021 | 5.4:+54205701 | MS.gene88718:CDS |
| TGGAGGTATAGGCCGAAGGA+TGG | 0.536112 | 5.4:+54205222 | MS.gene88718:CDS |
| TACTTGGTTTATGTGGGTGG+TGG | 0.539901 | 5.4:+54205498 | MS.gene88718:CDS |
| TCTCTCAACCTTACGGGACA+AGG | 0.545064 | 5.4:+54206091 | MS.gene88718:CDS |
| GATACCATCCTTGCAATCAC+TGG | 0.552369 | 5.4:-54206369 | None:intergenic |
| TTGACCAGTGATTGCAAGGA+TGG | 0.552652 | 5.4:+54206365 | MS.gene88718:CDS |
| GTTTAGTGTTGTTAGTATGG+GGG | 0.554390 | 5.4:+54205308 | MS.gene88718:CDS |
| GAAGAGGAAGAAGAATCAAA+AGG | 0.556632 | 5.4:+54205356 | MS.gene88718:CDS |
| GGCATTTGATATGGAAATCG+AGG | 0.561183 | 5.4:+54205931 | MS.gene88718:CDS |
| GTTCATTTGTAGCAATGTAG+AGG | 0.561206 | 5.4:-54206130 | None:intergenic |
| CGGCGAGACTAATGAGGCTG+AGG | 0.563477 | 5.4:+54205041 | MS.gene88718:CDS |
| AAATACTTGGTTTATGTGGG+TGG | 0.565916 | 5.4:+54205495 | MS.gene88718:CDS |
| AGAGCATGAATCATTTCTTG+TGG | 0.568634 | 5.4:+54205532 | MS.gene88718:CDS |
| TATGACTGATGTTGATAACT+CGG | 0.569096 | 5.4:+54205014 | MS.gene88718:CDS |
| GTATAGGCCGAAGGATGGGA+AGG | 0.570064 | 5.4:+54205227 | MS.gene88718:CDS |
| ACTCAGTTCATGTTGTGAGA+GGG | 0.571566 | 5.4:+54206006 | MS.gene88718:CDS |
| AGACAGAATCCGTTGTTCAA+AGG | 0.574269 | 5.4:+54205904 | MS.gene88718:CDS |
| GACTCAGTTCATGTTGTGAG+AGG | 0.575510 | 5.4:+54206005 | MS.gene88718:CDS |
| ATGCCATGGCCTTTGAACAA+CGG | 0.582631 | 5.4:-54205913 | None:intergenic |
| GTTGAATACTTATCCTTCAG+AGG | 0.583499 | 5.4:-54206173 | None:intergenic |
| CAGCCTCCCTGTAGTTGAGT+CGG | 0.583590 | 5.4:+54205452 | MS.gene88718:CDS |
| AATCCGTTGTTCAAAGGCCA+TGG | 0.583673 | 5.4:+54205910 | MS.gene88718:CDS |
| GATGTTGATAACTCGGTTAG+CGG | 0.585015 | 5.4:+54205021 | MS.gene88718:CDS |
| CTCAGTTCATGTTGTGAGAG+GGG | 0.585625 | 5.4:+54206007 | MS.gene88718:CDS |
| TAAGGTTGAGAGAAGTGCAT+CGG | 0.587272 | 5.4:-54206081 | None:intergenic |
| ATACCTCCACGCCATATCAC+AGG | 0.588167 | 5.4:-54205208 | None:intergenic |
| AATAGCTTCAAGGTTAAACT+GGG | 0.590944 | 5.4:+54205977 | MS.gene88718:CDS |
| GAGACAACAAAACTCAATAA+CGG | 0.592530 | 5.4:+54206251 | MS.gene88718:CDS |
| GGAGGTATAGGCCGAAGGAT+GGG | 0.595782 | 5.4:+54205223 | MS.gene88718:CDS |
| GGTTAGCGGCGAGACTAATG+AGG | 0.597233 | 5.4:+54205035 | MS.gene88718:CDS |
| TCGGAAAGTTCATTTAGTCA+AGG | 0.597241 | 5.4:+54205471 | MS.gene88718:CDS |
| TCATCTGGTCGAGGATTACA+GGG | 0.598635 | 5.4:+54205782 | MS.gene88718:CDS |
| CACTTCATCGAAGCAAGATG+AGG | 0.598938 | 5.4:+54205638 | MS.gene88718:CDS |
| GCCGAAGGATGGGAAGGCTG+CGG | 0.599090 | 5.4:+54205233 | MS.gene88718:CDS |
| TGATTTGACCAGTGATTGCA+AGG | 0.600244 | 5.4:+54206361 | MS.gene88718:CDS |
| ATCATACTGGTATGAATGCG+AGG | 0.603806 | 5.4:+54205334 | MS.gene88718:CDS |
| GGCGGCGTCTGTGTTGGACA+AGG | 0.610283 | 5.4:+54205707 | MS.gene88718:CDS |
| TTAGTTGAAGGATGAAACAA+AGG | 0.610506 | 5.4:-54204874 | None:intergenic |
| GATGAATCTTCATCTGGTCG+AGG | 0.613587 | 5.4:+54205773 | MS.gene88718:CDS |
| CTGAGGTGACTCAGTGTGAG+GGG | 0.614034 | 5.4:+54205058 | MS.gene88718:CDS |
| GGCGTGGAGGTATAGGCCGA+AGG | 0.618753 | 5.4:+54205218 | MS.gene88718:CDS |
| GATTTCCATATCAAATGCCA+TGG | 0.621642 | 5.4:-54205927 | None:intergenic |
| TCATCGAAGCAAGATGAGGA+AGG | 0.621897 | 5.4:+54205642 | MS.gene88718:CDS |
| AACTTTCCGACTCAACTACA+GGG | 0.628434 | 5.4:-54205458 | None:intergenic |
| AGTGATTAAGAATGTTGGGT+CGG | 0.631650 | 5.4:+54204707 | None:intergenic |
| TCAGTACCTAAACCGAACAT+TGG | 0.632278 | 5.4:+54205581 | MS.gene88718:CDS |
| TTTGGGATGAGACTAGTGAA+TGG | 0.636894 | 5.4:+54206222 | MS.gene88718:CDS |
| GCTGAGGTGACTCAGTGTGA+GGG | 0.644517 | 5.4:+54205057 | MS.gene88718:CDS |
| TTGTGAGAGGGGAAAAGGCG+AGG | 0.644621 | 5.4:+54206018 | MS.gene88718:CDS |
| GGTTGAGAGAAGTGCATCGG+GGG | 0.648446 | 5.4:-54206078 | None:intergenic |
| CAGGTACTATCATTCGAACC+CGG | 0.649356 | 5.4:-54205189 | None:intergenic |
| TATTGGTATAGAGTCTGCGA+AGG | 0.653670 | 5.4:+54205879 | MS.gene88718:CDS |
| GGCTGAGGTGACTCAGTGTG+AGG | 0.673349 | 5.4:+54205056 | MS.gene88718:CDS |
| ATAGTACCTGTGATATGGCG+TGG | 0.678671 | 5.4:+54205202 | MS.gene88718:CDS |
| TATTGATTGTAAGAACCCTG+AGG | 0.692288 | 5.4:+54205092 | MS.gene88718:CDS |
| TTACGGGACAAGGTTGACGA+AGG | 0.696825 | 5.4:+54206101 | MS.gene88718:CDS |
| GTACCTGTGATATGGCGTGG+AGG | 0.742102 | 5.4:+54205205 | MS.gene88718:CDS |
| AGGTTGAGAGAAGTGCATCG+GGG | 0.747201 | 5.4:-54206079 | None:intergenic |
| TTTCCGACTCAACTACAGGG+AGG | 0.771122 | 5.4:-54205455 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATGTTCATTTTTATAAATTT+GGG | + | chr5.4:54205157-54205176 | MS.gene88718:CDS | 10.0% |
| !!! | GATGTTCATTTTTATAAATT+TGG | + | chr5.4:54205156-54205175 | MS.gene88718:CDS | 15.0% |
| ! | AATGTTGTAATCTTAGTTGA+AGG | - | chr5.4:54204889-54204908 | None:intergenic | 25.0% |
| ! | ATTTAGTCAAGGTAAATACT+TGG | + | chr5.4:54205482-54205501 | MS.gene88718:CDS | 25.0% |
| !!! | TGTTTTGGTTTTCACTATTA+TGG | + | chr5.4:54204828-54204847 | MS.gene88718:CDS | 25.0% |
| !!! | TGTTTTTCAGTGTTTGTTAT+TGG | + | chr5.4:54204805-54204824 | MS.gene88718:CDS | 25.0% |
| AAAACAAACATTCCCAATCA+TGG | - | chr5.4:54204791-54204810 | None:intergenic | 30.0% | |
| AATAGCTTCAAGGTTAAACT+GGG | + | chr5.4:54205977-54205996 | MS.gene88718:CDS | 30.0% | |
| ACTCTATACCAATAGTTATC+AGG | - | chr5.4:54205873-54205892 | None:intergenic | 30.0% | |
| AGGTAAATACTTGGTTTATG+TGG | + | chr5.4:54205491-54205510 | MS.gene88718:CDS | 30.0% | |
| ATCAGACTTAAAAGTAGCAT+TGG | - | chr5.4:54204926-54204945 | None:intergenic | 30.0% | |
| ATCATCAAATGAAAAACCTC+AGG | - | chr5.4:54205111-54205130 | None:intergenic | 30.0% | |
| ATGTAACCATCAAATTCTAC+TGG | - | chr5.4:54206281-54206300 | None:intergenic | 30.0% | |
| CAAAAACACTTCTGTATCAA+TGG | - | chr5.4:54206312-54206331 | None:intergenic | 30.0% | |
| GAATTTAGGTCAGAATACTA+TGG | + | chr5.4:54204765-54204784 | MS.gene88718:CDS | 30.0% | |
| GAGACAACAAAACTCAATAA+CGG | + | chr5.4:54206251-54206270 | MS.gene88718:CDS | 30.0% | |
| GCTTTAATCATGAGAAAGTT+TGG | + | chr5.4:54205837-54205856 | MS.gene88718:CDS | 30.0% | |
| GGTAAATACTTGGTTTATGT+GGG | + | chr5.4:54205492-54205511 | MS.gene88718:CDS | 30.0% | |
| TCAAATCCATAACCAATGTT+CGG | - | chr5.4:54205596-54205615 | None:intergenic | 30.0% | |
| TCATCAAATGAAAAACCTCA+GGG | - | chr5.4:54205110-54205129 | None:intergenic | 30.0% | |
| TTAGTTGAAGGATGAAACAA+AGG | - | chr5.4:54204877-54204896 | None:intergenic | 30.0% | |
| TTCACATCCATAAGTTTCAT+TGG | - | chr5.4:54205813-54205832 | None:intergenic | 30.0% | |
| ! | ACGTTTAGTGTTGTTAGTAT+GGG | + | chr5.4:54205306-54205325 | MS.gene88718:CDS | 30.0% |
| ! | CGTTTTATAAGGATTATAGG+AGG | + | chr5.4:54205256-54205275 | MS.gene88718:CDS | 30.0% |
| ! | GAAAACTGGTGAAGATTTTA+TGG | + | chr5.4:54204957-54204976 | MS.gene88718:CDS | 30.0% |
| ! | TACGTTTAGTGTTGTTAGTA+TGG | + | chr5.4:54205305-54205324 | MS.gene88718:CDS | 30.0% |
| ! | TCATTTTCTCAACGAGTATA+AGG | + | chr5.4:54206196-54206215 | MS.gene88718:CDS | 30.0% |
| !! | CTTTGATTTTGAGCATTTGA+AGG | + | chr5.4:54205683-54205702 | MS.gene88718:CDS | 30.0% |
| !! | TATGACTGATGTTGATAACT+CGG | + | chr5.4:54205014-54205033 | MS.gene88718:CDS | 30.0% |
| !!! | AGGTTTTTCATTTGATGATG+AGG | + | chr5.4:54205112-54205131 | MS.gene88718:CDS | 30.0% |
| !!! | AGTGTTTGTTATTGGTGTTT+TGG | + | chr5.4:54204813-54204832 | MS.gene88718:CDS | 30.0% |
| !!! | GGTTTTTCATTTGATGATGA+GGG | + | chr5.4:54205113-54205132 | MS.gene88718:CDS | 30.0% |
| !!! | GTTATTGAGTTTTGTTGTCT+CGG | - | chr5.4:54206252-54206271 | None:intergenic | 30.0% |
| AAATACTTGGTTTATGTGGG+TGG | + | chr5.4:54205495-54205514 | MS.gene88718:CDS | 35.0% | |
| AGAATACTATGGCCATGATT+GGG | + | chr5.4:54204776-54204795 | MS.gene88718:CDS | 35.0% | |
| AGAGCATGAATCATTTCTTG+TGG | + | chr5.4:54205532-54205551 | MS.gene88718:CDS | 35.0% | |
| AGTAAGTGGCAACAAAAAGA+TGG | + | chr5.4:54205750-54205769 | MS.gene88718:CDS | 35.0% | |
| ATCTTTCTTTGATCCTCTGA+AGG | + | chr5.4:54206160-54206179 | MS.gene88718:CDS | 35.0% | |
| CAACGAGTATAAGGAACTTT+GGG | + | chr5.4:54206205-54206224 | MS.gene88718:CDS | 35.0% | |
| CAATAGCTTCAAGGTTAAAC+TGG | + | chr5.4:54205976-54205995 | MS.gene88718:CDS | 35.0% | |
| GAAGAGGAAGAAGAATCAAA+AGG | + | chr5.4:54205356-54205375 | MS.gene88718:CDS | 35.0% | |
| GAATGATAGTACCTGTGATA+TGG | + | chr5.4:54205197-54205216 | MS.gene88718:CDS | 35.0% | |
| GATAACAGTGTTGTGAAAAC+TGG | + | chr5.4:54204943-54204962 | MS.gene88718:CDS | 35.0% | |
| GATTTCCATATCAAATGCCA+TGG | - | chr5.4:54205930-54205949 | None:intergenic | 35.0% | |
| GTAAGTGGCAACAAAAAGAT+GGG | + | chr5.4:54205751-54205770 | MS.gene88718:CDS | 35.0% | |
| GTATTCTGACCTAAATTCTC+TGG | - | chr5.4:54204763-54204782 | None:intergenic | 35.0% | |
| GTTCATTTGTAGCAATGTAG+AGG | - | chr5.4:54206133-54206152 | None:intergenic | 35.0% | |
| GTTGAATACTTATCCTTCAG+AGG | - | chr5.4:54206176-54206195 | None:intergenic | 35.0% | |
| TAAATTTGGGAAACCTGTTC+CGG | + | chr5.4:54205170-54205189 | MS.gene88718:CDS | 35.0% | |
| TCAACGAGTATAAGGAACTT+TGG | + | chr5.4:54206204-54206223 | MS.gene88718:CDS | 35.0% | |
| TCAGGGTTCTTACAATCAAT+AGG | - | chr5.4:54205093-54205112 | None:intergenic | 35.0% | |
| TCGGAAAGTTCATTTAGTCA+AGG | + | chr5.4:54205471-54205490 | MS.gene88718:CDS | 35.0% | |
| TTCACAAATTCTCCAACAAC+CGG | - | chr5.4:54205429-54205448 | None:intergenic | 35.0% | |
| ! | ATGAGGAAGGCAAAGATTTT+AGG | + | chr5.4:54205655-54205674 | MS.gene88718:CDS | 35.0% |
| ! | CGGCGTTTTATAAGGATTAT+AGG | + | chr5.4:54205253-54205272 | MS.gene88718:CDS | 35.0% |
| ! | CGTTTAGTGTTGTTAGTATG+GGG | + | chr5.4:54205307-54205326 | MS.gene88718:CDS | 35.0% |
| ! | GTTTAGTGTTGTTAGTATGG+GGG | + | chr5.4:54205308-54205327 | MS.gene88718:CDS | 35.0% |
| ! | TATTGATTGTAAGAACCCTG+AGG | + | chr5.4:54205092-54205111 | MS.gene88718:CDS | 35.0% |
| ! | TGATTTTGAGCATTTGAAGG+AGG | + | chr5.4:54205686-54205705 | MS.gene88718:CDS | 35.0% |
| !!! | GATACAGAAGTGTTTTTGAG+AGG | + | chr5.4:54206314-54206333 | MS.gene88718:CDS | 35.0% |
| AAAGGCCATGGCATTTGATA+TGG | + | chr5.4:54205922-54205941 | MS.gene88718:CDS | 40.0% | |
| AAATTTGGGAAACCTGTTCC+GGG | + | chr5.4:54205171-54205190 | MS.gene88718:CDS | 40.0% | |
| AACTTTCCGACTCAACTACA+GGG | - | chr5.4:54205461-54205480 | None:intergenic | 40.0% | |
| ACTCAGTTCATGTTGTGAGA+GGG | + | chr5.4:54206006-54206025 | MS.gene88718:CDS | 40.0% | |
| AGACAGAATCCGTTGTTCAA+AGG | + | chr5.4:54205904-54205923 | MS.gene88718:CDS | 40.0% | |
| AGATGGGATGAATCTTCATC+TGG | + | chr5.4:54205767-54205786 | MS.gene88718:CDS | 40.0% | |
| AGGAAGAAGAATCAAAAGGC+TGG | + | chr5.4:54205360-54205379 | MS.gene88718:CDS | 40.0% | |
| AGTATGGGGGATTATCATAC+TGG | + | chr5.4:54205321-54205340 | MS.gene88718:CDS | 40.0% | |
| ATCATACTGGTATGAATGCG+AGG | + | chr5.4:54205334-54205353 | MS.gene88718:CDS | 40.0% | |
| ATGCACTTCTCTCAACCTTA+CGG | + | chr5.4:54206084-54206103 | MS.gene88718:CDS | 40.0% | |
| ATGTCGAGCCTGATAACTAT+TGG | + | chr5.4:54205862-54205881 | MS.gene88718:CDS | 40.0% | |
| CAGAATACTATGGCCATGAT+TGG | + | chr5.4:54204775-54204794 | MS.gene88718:CDS | 40.0% | |
| CCATAACCAATGTTCGGTTT+AGG | - | chr5.4:54205590-54205609 | None:intergenic | 40.0% | |
| GAGGTATGTGCATCAAGATT+TGG | - | chr5.4:54206059-54206078 | None:intergenic | 40.0% | |
| GCTACTAATGAGTCTGTGTT+TGG | + | chr5.4:54204979-54204998 | MS.gene88718:CDS | 40.0% | |
| GGATCAAAGAAAGATGCATC+AGG | - | chr5.4:54206155-54206174 | None:intergenic | 40.0% | |
| GGATGAAACAAAGGATCTTC+AGG | - | chr5.4:54204868-54204887 | None:intergenic | 40.0% | |
| GGCATTTGATATGGAAATCG+AGG | + | chr5.4:54205931-54205950 | MS.gene88718:CDS | 40.0% | |
| GGTCACACCAATGAAACTTA+TGG | + | chr5.4:54205803-54205822 | MS.gene88718:CDS | 40.0% | |
| GGTTTCAGACCAGAGAATTT+AGG | + | chr5.4:54204751-54204770 | MS.gene88718:CDS | 40.0% | |
| TAGTGTCTGCAATAGCTTCA+AGG | + | chr5.4:54205967-54205986 | MS.gene88718:CDS | 40.0% | |
| TATTGGTATAGAGTCTGCGA+AGG | + | chr5.4:54205879-54205898 | MS.gene88718:CDS | 40.0% | |
| TCAGTACCTAAACCGAACAT+TGG | + | chr5.4:54205581-54205600 | MS.gene88718:CDS | 40.0% | |
| TGATTTGACCAGTGATTGCA+AGG | + | chr5.4:54206361-54206380 | MS.gene88718:CDS | 40.0% | |
| ! | CCTAAACCGAACATTGGTTA+TGG | + | chr5.4:54205587-54205606 | MS.gene88718:CDS | 40.0% |
| ! | GTTGGACAAGGATCAGTTTT+GGG | + | chr5.4:54205719-54205738 | MS.gene88718:CDS | 40.0% |
| ! | TAAGGTTGAGAGAAGTGCAT+CGG | - | chr5.4:54206084-54206103 | None:intergenic | 40.0% |
| ! | TGTTGGACAAGGATCAGTTT+TGG | + | chr5.4:54205718-54205737 | MS.gene88718:CDS | 40.0% |
| ! | TTTGGGATGAGACTAGTGAA+TGG | + | chr5.4:54206222-54206241 | MS.gene88718:CDS | 40.0% |
| ! | TTTTGAGCATTTGAAGGAGG+CGG | + | chr5.4:54205689-54205708 | MS.gene88718:CDS | 40.0% |
| !! | GATGTTGATAACTCGGTTAG+CGG | + | chr5.4:54205021-54205040 | MS.gene88718:CDS | 40.0% |
| AATCCGTTGTTCAAAGGCCA+TGG | + | chr5.4:54205910-54205929 | MS.gene88718:CDS | 45.0% | |
| AGAGAAGACATCGTCGAATC+TGG | + | chr5.4:54205386-54205405 | MS.gene88718:CDS | 45.0% | |
| ATAGTACCTGTGATATGGCG+TGG | + | chr5.4:54205202-54205221 | MS.gene88718:CDS | 45.0% | |
| ATGCCATGGCCTTTGAACAA+CGG | - | chr5.4:54205916-54205935 | None:intergenic | 45.0% | |
| ATGGAAATCGAGGCGATTGA+TGG | + | chr5.4:54205941-54205960 | MS.gene88718:CDS | 45.0% | |
| CACTTCATCGAAGCAAGATG+AGG | + | chr5.4:54205638-54205657 | MS.gene88718:CDS | 45.0% | |
| CAGGTACTATCATTCGAACC+CGG | - | chr5.4:54205192-54205211 | None:intergenic | 45.0% | |
| CTCAGTTCATGTTGTGAGAG+GGG | + | chr5.4:54206007-54206026 | MS.gene88718:CDS | 45.0% | |
| GAACTTTCCGACTCAACTAC+AGG | - | chr5.4:54205462-54205481 | None:intergenic | 45.0% | |
| GACTCAGTTCATGTTGTGAG+AGG | + | chr5.4:54206005-54206024 | MS.gene88718:CDS | 45.0% | |
| GATACCATCCTTGCAATCAC+TGG | - | chr5.4:54206372-54206391 | None:intergenic | 45.0% | |
| GATGAATCTTCATCTGGTCG+AGG | + | chr5.4:54205773-54205792 | MS.gene88718:CDS | 45.0% | |
| GGCGTTCCAGTAGAATTTGA+TGG | + | chr5.4:54206272-54206291 | MS.gene88718:CDS | 45.0% | |
| TACTTGGTTTATGTGGGTGG+TGG | + | chr5.4:54205498-54205517 | MS.gene88718:CDS | 45.0% | |
| TCATCGAAGCAAGATGAGGA+AGG | + | chr5.4:54205642-54205661 | MS.gene88718:CDS | 45.0% | |
| TCATCTGGTCGAGGATTACA+GGG | + | chr5.4:54205782-54205801 | MS.gene88718:CDS | 45.0% | |
| TCATGTTGTGAGAGGGGAAA+AGG | + | chr5.4:54206013-54206032 | MS.gene88718:CDS | 45.0% | |
| TGCACTTCTCTCAACCTTAC+GGG | + | chr5.4:54206085-54206104 | MS.gene88718:CDS | 45.0% | |
| TGGAGTTTCTTGTGTGCTCT+AGG | + | chr5.4:54205552-54205571 | MS.gene88718:CDS | 45.0% | |
| TTCATCTGGTCGAGGATTAC+AGG | + | chr5.4:54205781-54205800 | MS.gene88718:CDS | 45.0% | |
| TTGACCAGTGATTGCAAGGA+TGG | + | chr5.4:54206365-54206384 | MS.gene88718:CDS | 45.0% | |
| TTTGGGCAGATTGGAGTAAG+TGG | + | chr5.4:54205736-54205755 | MS.gene88718:CDS | 45.0% | |
| ! | AAGGTTGAGAGAAGTGCATC+GGG | - | chr5.4:54206083-54206102 | None:intergenic | 45.0% |
| ! | TCTGCATTTTCTAGACCTGG+AGG | + | chr5.4:54204730-54204749 | MS.gene88718:CDS | 45.0% |
| !! | TTTATGTGGGTGGTGGAGAT+AGG | + | chr5.4:54205505-54205524 | MS.gene88718:CDS | 45.0% |
| !!! | AGGATCAGTTTTGGGCAGAT+TGG | + | chr5.4:54205727-54205746 | MS.gene88718:CDS | 45.0% |
| ACTCAGTGTGAGGGGAATTC+AGG | + | chr5.4:54205066-54205085 | MS.gene88718:CDS | 50.0% | |
| AGGCGAGGAACAAAGAGCTT+TGG | + | chr5.4:54206033-54206052 | MS.gene88718:CDS | 50.0% | |
| ATACCTCCACGCCATATCAC+AGG | - | chr5.4:54205211-54205230 | None:intergenic | 50.0% | |
| CTATCATTCGAACCCGGAAC+AGG | - | chr5.4:54205186-54205205 | None:intergenic | 50.0% | |
| CTGGTATGAATGCGAGGAAG+AGG | + | chr5.4:54205340-54205359 | MS.gene88718:CDS | 50.0% | |
| GACATCGTCGAATCTGGATC+AGG | + | chr5.4:54205392-54205411 | MS.gene88718:CDS | 50.0% | |
| TCTCTCAACCTTACGGGACA+AGG | + | chr5.4:54206091-54206110 | MS.gene88718:CDS | 50.0% | |
| TTACGGGACAAGGTTGACGA+AGG | + | chr5.4:54206101-54206120 | MS.gene88718:CDS | 50.0% | |
| TTCGTCAACCTTGTCCCGTA+AGG | - | chr5.4:54206102-54206121 | None:intergenic | 50.0% | |
| TTTCCGACTCAACTACAGGG+AGG | - | chr5.4:54205458-54205477 | None:intergenic | 50.0% | |
| ! | AGGTTGAGAGAAGTGCATCG+GGG | - | chr5.4:54206082-54206101 | None:intergenic | 50.0% |
| ! | CGGTCTGCATTTTCTAGACC+TGG | + | chr5.4:54204727-54204746 | MS.gene88718:CDS | 50.0% |
| ! | GAAGGCTGCGGCGTTTTATA+AGG | + | chr5.4:54205245-54205264 | MS.gene88718:CDS | 50.0% |
| ! | GTGATATGGCGTGGAGGTAT+AGG | + | chr5.4:54205211-54205230 | MS.gene88718:CDS | 50.0% |
| ! | TTCTCTGGTCTGAAACCTCC+AGG | - | chr5.4:54204748-54204767 | None:intergenic | 50.0% |
| CAGCCTCCCTGTAGTTGAGT+CGG | + | chr5.4:54205452-54205471 | MS.gene88718:CDS | 55.0% | |
| CTGAGGTGACTCAGTGTGAG+GGG | + | chr5.4:54205058-54205077 | MS.gene88718:CDS | 55.0% | |
| GCTGAGGTGACTCAGTGTGA+GGG | + | chr5.4:54205057-54205076 | MS.gene88718:CDS | 55.0% | |
| GGATCAGGTGAATGCGTTGC+CGG | + | chr5.4:54205407-54205426 | MS.gene88718:CDS | 55.0% | |
| GGTTAGCGGCGAGACTAATG+AGG | + | chr5.4:54205035-54205054 | MS.gene88718:CDS | 55.0% | |
| GTACCTGTGATATGGCGTGG+AGG | + | chr5.4:54205205-54205224 | MS.gene88718:CDS | 55.0% | |
| GTATAGGCCGAAGGATGGGA+AGG | + | chr5.4:54205227-54205246 | MS.gene88718:CDS | 55.0% | |
| TTGTGAGAGGGGAAAAGGCG+AGG | + | chr5.4:54206018-54206037 | MS.gene88718:CDS | 55.0% | |
| ! | GGAGGTATAGGCCGAAGGAT+GGG | + | chr5.4:54205223-54205242 | MS.gene88718:CDS | 55.0% |
| ! | GGTTGAGAGAAGTGCATCGG+GGG | - | chr5.4:54206081-54206100 | None:intergenic | 55.0% |
| ! | GTGAATGCGTTGCCGGTTGT+TGG | + | chr5.4:54205414-54205433 | MS.gene88718:CDS | 55.0% |
| ! | TGGAGGTATAGGCCGAAGGA+TGG | + | chr5.4:54205222-54205241 | MS.gene88718:CDS | 55.0% |
| CGGCGAGACTAATGAGGCTG+AGG | + | chr5.4:54205041-54205060 | MS.gene88718:CDS | 60.0% | |
| GGCTGAGGTGACTCAGTGTG+AGG | + | chr5.4:54205056-54205075 | MS.gene88718:CDS | 60.0% | |
| ! | TGAGAGAAGTGCATCGGGGG+AGG | - | chr5.4:54206078-54206097 | None:intergenic | 60.0% |
| GAAGGAGGCGGCGTCTGTGT+TGG | + | chr5.4:54205701-54205720 | MS.gene88718:CDS | 65.0% | |
| GCCGAAGGATGGGAAGGCTG+CGG | + | chr5.4:54205233-54205252 | MS.gene88718:CDS | 65.0% | |
| GCCGCAGCCTTCCCATCCTT+CGG | - | chr5.4:54205237-54205256 | None:intergenic | 65.0% | |
| GGCGGCGTCTGTGTTGGACA+AGG | + | chr5.4:54205707-54205726 | MS.gene88718:CDS | 65.0% | |
| ! | GGCGTGGAGGTATAGGCCGA+AGG | + | chr5.4:54205218-54205237 | MS.gene88718:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 54204718 | 54206418 | 54204718 | ID=MS.gene88718 |
| chr5.4 | mRNA | 54204718 | 54206418 | 54204718 | ID=MS.gene88718.t1;Parent=MS.gene88718 |
| chr5.4 | exon | 54204718 | 54206418 | 54204718 | ID=MS.gene88718.t1.exon1;Parent=MS.gene88718.t1 |
| chr5.4 | CDS | 54204718 | 54206418 | 54204718 | ID=cds.MS.gene88718.t1;Parent=MS.gene88718.t1 |
| Gene Sequence |
| Protein sequence |
