AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49436


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49436.t1 MTR_1g095000 98.286 175 3 0 1 175 106 280 5.86e-127 358
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49436.t1 AT2G39000 74.419 172 44 0 1 172 55 226 5.02e-97 280
MS.gene49436.t1 AT2G39000 74.419 172 44 0 1 172 111 282 9.40e-97 282
MS.gene49436.t1 AT2G39000 77.500 80 18 0 1 80 111 190 4.80e-41 137
MS.gene49436.t1 AT2G39000 77.215 79 18 0 1 79 111 189 2.88e-40 136

Find 43 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGATCAAACTTTCTTATT+TGG 0.151315 1.1:-68465525 MS.gene49436:CDS
ACCTGTCTGGTTGCTGTTAT+TGG 0.195810 1.1:-68465555 MS.gene49436:CDS
TTTCAAATCTATAAAGGTTC+CGG 0.297267 1.1:-68464135 MS.gene49436:CDS
TGTCGCAGAGGCCCCTTTCT+AGG 0.313734 1.1:+68465402 None:intergenic
GCAAGGTTTCAAATCTATAA+AGG 0.323365 1.1:-68464141 MS.gene49436:CDS
GGCACGACATCCCCAACTTC+TGG 0.376576 1.1:+68464217 None:intergenic
AGTGAAGACTTCAAGATTGC+GGG 0.393136 1.1:-68465501 MS.gene49436:CDS
TTCAAATCTATAAAGGTTCC+GGG 0.414198 1.1:-68464134 MS.gene49436:CDS
TGTGTTGAGGATGGATAGAT+TGG 0.415569 1.1:-68465622 None:intergenic
AGAATCACAAGCCAGAAGTT+GGG 0.428773 1.1:-68464228 MS.gene49436:CDS
AGGTTCCGGGAGGAGCTAAC+TGG 0.431391 1.1:-68464121 MS.gene49436:CDS
AGCTAAACAATTGGTGGCTA+AGG 0.435007 1.1:-68464252 MS.gene49436:CDS
TGCAGACTTTCTACCTAGAA+AGG 0.446932 1.1:-68465415 MS.gene49436:CDS
ATGAAGTTGAACTTCACATC+TGG 0.450823 1.1:+68464080 None:intergenic
CAGAATCACAAGCCAGAAGT+TGG 0.456663 1.1:-68464229 MS.gene49436:CDS
GCAGGATTCTCTTTACCAAA+TGG 0.460775 1.1:-68465588 MS.gene49436:CDS
GAAAGGAATAGCTAAACAAT+TGG 0.467703 1.1:-68464261 MS.gene49436:CDS
GCAGACTTTCTACCTAGAAA+GGG 0.481732 1.1:-68465414 MS.gene49436:CDS
TAGATTTGAAACCTTGCCCT+TGG 0.481800 1.1:+68464147 None:intergenic
TGGTATAACTTTGTTGCCGC+AGG 0.491255 1.1:+68464167 None:intergenic
AGATTGGTGGCTATGTTAGC+AGG 0.496959 1.1:-68465606 MS.gene49436:CDS
CTCAACAAGGGATATGTGGC+TGG 0.532427 1.1:-68465459 MS.gene49436:CDS
GCCAATAACAGCAACCAGAC+AGG 0.535502 1.1:+68465554 None:intergenic
GAATCACAAGCCAGAAGTTG+GGG 0.540089 1.1:-68464227 MS.gene49436:CDS
AAGCCTCAACAAGGGATATG+TGG 0.555870 1.1:-68465463 MS.gene49436:CDS
AGGAATAGCTAAACAATTGG+TGG 0.557067 1.1:-68464258 MS.gene49436:CDS
TGGTTGCAAAAGAACCTGTC+TGG 0.566369 1.1:-68465568 MS.gene49436:CDS
AAGTGAAGACTTCAAGATTG+CGG 0.578803 1.1:-68465502 MS.gene49436:CDS
GTTGAGGATGGATAGATTGG+TGG 0.579094 1.1:-68465619 None:intergenic
AGGGGCCTCTGCGACAGAGA+AGG 0.581862 1.1:-68465395 MS.gene49436:intron
TGAGGCCAGTTAGCTCCTCC+CGG 0.584487 1.1:+68464116 None:intergenic
CAGCCACATATCCCTTGTTG+AGG 0.606889 1.1:+68465460 None:intergenic
ACAAAGTTATACCAAGGGCA+AGG 0.614543 1.1:-68464158 MS.gene49436:CDS
TGGGAAATTAAGCCTCAACA+AGG 0.631474 1.1:-68465472 MS.gene49436:CDS
TAAATCGCAGTGTAATGCAA+TGG 0.632285 1.1:+68464196 None:intergenic
CAGACTTTCTACCTAGAAAG+GGG 0.636163 1.1:-68465413 MS.gene49436:CDS
AATTGCATACATATCAAATG+TGG 0.643430 1.1:-68464306 MS.gene49436:CDS
CGGCAACAAAGTTATACCAA+GGG 0.644825 1.1:-68464163 MS.gene49436:CDS
GCGGCAACAAAGTTATACCA+AGG 0.651915 1.1:-68464164 MS.gene49436:CDS
ACTCACCTTCTCTGTCGCAG+AGG 0.668717 1.1:+68465390 None:intergenic
GGGAAATTAAGCCTCAACAA+GGG 0.686786 1.1:-68465471 MS.gene49436:CDS
CTGCGATTTACGAAATCCTG+CGG 0.698179 1.1:-68464183 MS.gene49436:CDS
AAATCTATAAAGGTTCCGGG+AGG 0.724022 1.1:-68464131 MS.gene49436:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATGTATAAAAACTATTGT+AGG - chr1.1:68465324-68465343 MS.gene49436:intron 15.0%
!!! ATAAAAAGTAGTTTGTATTT+TGG + chr1.1:68464530-68464549 None:intergenic 15.0%
!!! CAATTAATTTTAACATGTTT+TGG + chr1.1:68464576-68464595 None:intergenic 15.0%
!!! TTGTATCTTTTTCTTATAAT+TGG + chr1.1:68464771-68464790 None:intergenic 15.0%
!! ATCAAAATTATAGCATTAAG+TGG - chr1.1:68464922-68464941 MS.gene49436:intron 20.0%
!! ATGCATTAAAAGACAAAATA+AGG + chr1.1:68464830-68464849 None:intergenic 20.0%
!! TGCATTAAAAGACAAAATAA+GGG + chr1.1:68464829-68464848 None:intergenic 20.0%
!!! ATCTTTTTCTTATAATTGGT+AGG + chr1.1:68464767-68464786 None:intergenic 20.0%
!!! ATTTAATTTAGAGTCATGTT+TGG - chr1.1:68464891-68464910 MS.gene49436:intron 20.0%
!!! TATGTTTCTAACTATGTATA+AGG - chr1.1:68465227-68465246 MS.gene49436:intron 20.0%
!!! TTTAATTTAGAGTCATGTTT+GGG - chr1.1:68464892-68464911 MS.gene49436:intron 20.0%
! AATTGCATACATATCAAATG+TGG - chr1.1:68465352-68465371 MS.gene49436:intron 25.0%
! AGAGAAAAATTTAGACAGAA+AGG - chr1.1:68465380-68465399 MS.gene49436:intron 25.0%
! AGATAACTTGAAAATTACAC+AGG + chr1.1:68464492-68464511 None:intergenic 25.0%
! ATCTATTCCATATAAACAGT+AGG - chr1.1:68464848-68464867 MS.gene49436:intron 25.0%
! CTTGATCAAACTTTCTTATT+TGG - chr1.1:68464133-68464152 MS.gene49436:CDS 25.0%
! TATAAAAACTATTGTAGGAC+TGG - chr1.1:68465329-68465348 MS.gene49436:intron 25.0%
! TTTCAAATCTATAAAGGTTC+CGG - chr1.1:68465523-68465542 MS.gene49436:CDS 25.0%
!! AACATAGTTTACCATTTTGA+AGG + chr1.1:68465213-68465232 None:intergenic 25.0%
!! ACATAGTTTACCATTTTGAA+GGG + chr1.1:68465212-68465231 None:intergenic 25.0%
!!! ACAAACTACTTTTTATCTAG+AGG - chr1.1:68464534-68464553 MS.gene49436:intron 25.0%
!!! ATTGTTTTTTTCTTTGACCA+GGG - chr1.1:68464961-68464980 MS.gene49436:intron 25.0%
!!! TAAAACTTGTTTCTGAATCT+GGG - chr1.1:68464291-68464310 MS.gene49436:CDS 25.0%
!!! TTAAAACTTGTTTCTGAATC+TGG - chr1.1:68464290-68464309 MS.gene49436:CDS 25.0%
ACTAGTAACATGAGTAAACT+AGG + chr1.1:68465278-68465297 None:intergenic 30.0%
GAAAATTACACAGGAATGAA+AGG + chr1.1:68464483-68464502 None:intergenic 30.0%
GAAAGGAATAGCTAAACAAT+TGG - chr1.1:68465397-68465416 MS.gene49436:CDS 30.0%
GCAAGGTTTCAAATCTATAA+AGG - chr1.1:68465517-68465536 MS.gene49436:CDS 30.0%
TCTAAATTAAATGCTCTTCG+AGG + chr1.1:68464883-68464902 None:intergenic 30.0%
TTCAAATCTATAAAGGTTCC+GGG - chr1.1:68465524-68465543 MS.gene49436:CDS 30.0%
!! TTCTGTTTTAAACAAGTGCA+AGG + chr1.1:68465090-68465109 None:intergenic 30.0%
!!! GATTGTTTTTTTCTTTGACC+AGG - chr1.1:68464960-68464979 MS.gene49436:intron 30.0%
AAGTGAAGACTTCAAGATTG+CGG - chr1.1:68464156-68464175 MS.gene49436:CDS 35.0%
AATTAAATGCTCTTCGAGGA+TGG + chr1.1:68464879-68464898 None:intergenic 35.0%
AGGAATAGCTAAACAATTGG+TGG - chr1.1:68465400-68465419 MS.gene49436:CDS 35.0%
AGTTATGTGATAAATGTCCC+TGG + chr1.1:68464981-68465000 None:intergenic 35.0%
ATGAAGTTGAACTTCACATC+TGG + chr1.1:68465581-68465600 None:intergenic 35.0%
GAAAATTGTTGAGACATGTC+TGG + chr1.1:68464628-68464647 None:intergenic 35.0%
GGTAACACCTACTGTTTATA+TGG + chr1.1:68464858-68464877 None:intergenic 35.0%
TAAATCGCAGTGTAATGCAA+TGG + chr1.1:68465465-68465484 None:intergenic 35.0%
TCTCTTCATATTGATCACTG+AGG - chr1.1:68465117-68465136 MS.gene49436:intron 35.0%
! CATTTTCTTATACACGAGAC+TGG + chr1.1:68464730-68464749 None:intergenic 35.0%
! TTCACATCTGGAGAAGTTTT+TGG + chr1.1:68465569-68465588 None:intergenic 35.0%
!! AGACAAGCTAGAAGCATTTA+AGG + chr1.1:68464682-68464701 None:intergenic 35.0%
AAATCTATAAAGGTTCCGGG+AGG - chr1.1:68465527-68465546 MS.gene49436:CDS 40.0%
ACAAAGTTATACCAAGGGCA+AGG - chr1.1:68465500-68465519 MS.gene49436:CDS 40.0%
AGAATCACAAGCCAGAAGTT+GGG - chr1.1:68465430-68465449 MS.gene49436:CDS 40.0%
AGTGAAGACTTCAAGATTGC+GGG - chr1.1:68464157-68464176 MS.gene49436:CDS 40.0%
ATTGATCACTGAGGCAAGAA+TGG - chr1.1:68465126-68465145 MS.gene49436:intron 40.0%
CAGACTTTCTACCTAGAAAG+GGG - chr1.1:68464245-68464264 MS.gene49436:CDS 40.0%
CGGCAACAAAGTTATACCAA+GGG - chr1.1:68465495-68465514 MS.gene49436:CDS 40.0%
GCAGACTTTCTACCTAGAAA+GGG - chr1.1:68464244-68464263 MS.gene49436:CDS 40.0%
GCAGGATTCTCTTTACCAAA+TGG - chr1.1:68464070-68464089 MS.gene49436:CDS 40.0%
GGGAAATTAAGCCTCAACAA+GGG - chr1.1:68464187-68464206 MS.gene49436:CDS 40.0%
TCCTGCATTACCCTTCAAAA+TGG - chr1.1:68465199-68465218 MS.gene49436:intron 40.0%
TGACATGCGATGAAAGAACA+TGG + chr1.1:68465042-68465061 None:intergenic 40.0%
TGCAGACTTTCTACCTAGAA+AGG - chr1.1:68464243-68464262 MS.gene49436:CDS 40.0%
TGGGAAATTAAGCCTCAACA+AGG - chr1.1:68464186-68464205 MS.gene49436:CDS 40.0%
TTCTTATACACGAGACTGGA+GGG + chr1.1:68464726-68464745 None:intergenic 40.0%
TTTCTTATACACGAGACTGG+AGG + chr1.1:68464727-68464746 None:intergenic 40.0%
! AGCTAAACAATTGGTGGCTA+AGG - chr1.1:68465406-68465425 MS.gene49436:CDS 40.0%
! CAGGTTCTTTTGCAACCATT+TGG + chr1.1:68464088-68464107 None:intergenic 40.0%
! TAGATTTGAAACCTTGCCCT+TGG + chr1.1:68465514-68465533 None:intergenic 40.0%
!! ACCATTTTGAAGGGTAATGC+AGG + chr1.1:68465203-68465222 None:intergenic 40.0%
!! CTGAATTCGCTTTTGTCTTC+CGG - chr1.1:68464599-68464618 MS.gene49436:intron 40.0%
!! TCAAGATTGCGGGTTTTGAT+GGG - chr1.1:68464167-68464186 MS.gene49436:CDS 40.0%
!! TTCAAGATTGCGGGTTTTGA+TGG - chr1.1:68464166-68464185 MS.gene49436:CDS 40.0%
!!! TCTGGAGAAGTTTTTGGTTG+AGG + chr1.1:68465563-68465582 None:intergenic 40.0%
AAGCCTCAACAAGGGATATG+TGG - chr1.1:68464195-68464214 MS.gene49436:CDS 45.0%
ACACAGGAATGAAAGGCAAG+CGG + chr1.1:68464476-68464495 None:intergenic 45.0%
CAGAATCACAAGCCAGAAGT+TGG - chr1.1:68465429-68465448 MS.gene49436:CDS 45.0%
CGCATGTCAATAGTTCTTCG+TGG - chr1.1:68465053-68465072 MS.gene49436:intron 45.0%
CTGCGATTTACGAAATCCTG+CGG - chr1.1:68465475-68465494 MS.gene49436:CDS 45.0%
GAATCACAAGCCAGAAGTTG+GGG - chr1.1:68465431-68465450 MS.gene49436:CDS 45.0%
GCGGCAACAAAGTTATACCA+AGG - chr1.1:68465494-68465513 MS.gene49436:CDS 45.0%
TGAATCTGGGTTTGTGAGTG+AGG - chr1.1:68464304-68464323 MS.gene49436:CDS 45.0%
TGGTATAACTTTGTTGCCGC+AGG + chr1.1:68465494-68465513 None:intergenic 45.0%
TGGTTGCAAAAGAACCTGTC+TGG - chr1.1:68464090-68464109 MS.gene49436:CDS 45.0%
! ACCTGTCTGGTTGCTGTTAT+TGG - chr1.1:68464103-68464122 MS.gene49436:CDS 45.0%
! AGATTGGTGGCTATGTTAGC+AGG - chr1.1:68464052-68464071 MS.gene49436:CDS 45.0%
!! CTGAGGCAAGAATGGACTTT+TGG - chr1.1:68465134-68465153 MS.gene49436:intron 45.0%
CAGCCACATATCCCTTGTTG+AGG + chr1.1:68464201-68464220 None:intergenic 50.0%
CTCAACAAGGGATATGTGGC+TGG - chr1.1:68464199-68464218 MS.gene49436:CDS 50.0%
GCCAATAACAGCAACCAGAC+AGG + chr1.1:68464107-68464126 None:intergenic 50.0%
GTTGAGACATGTCTGGACTC+CGG + chr1.1:68464621-68464640 None:intergenic 50.0%
ACTCACCTTCTCTGTCGCAG+AGG + chr1.1:68464271-68464290 None:intergenic 55.0%
! ACGAGACTGGAGGGAGTATG+TGG + chr1.1:68464717-68464736 None:intergenic 55.0%
AGGTTCCGGGAGGAGCTAAC+TGG - chr1.1:68465537-68465556 MS.gene49436:CDS 60.0%
GGCACGACATCCCCAACTTC+TGG + chr1.1:68465444-68465463 None:intergenic 60.0%
TGAGGCCAGTTAGCTCCTCC+CGG + chr1.1:68465545-68465564 None:intergenic 60.0%
TGTCGCAGAGGCCCCTTTCT+AGG + chr1.1:68464259-68464278 None:intergenic 60.0%
AGGGGCCTCTGCGACAGAGA+AGG - chr1.1:68464263-68464282 MS.gene49436:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 68464046 68465634 68464046 ID=MS.gene49436
chr1.1 mRNA 68464046 68465634 68464046 ID=MS.gene49436.t1;Parent=MS.gene49436
chr1.1 exon 68465396 68465634 68465396 ID=MS.gene49436.t1.exon1;Parent=MS.gene49436.t1
chr1.1 CDS 68465396 68465634 68465396 ID=cds.MS.gene49436.t1;Parent=MS.gene49436.t1
chr1.1 exon 68464046 68464334 68464046 ID=MS.gene49436.t1.exon2;Parent=MS.gene49436.t1
chr1.1 CDS 68464046 68464334 68464046 ID=cds.MS.gene49436.t1;Parent=MS.gene49436.t1
Gene Sequence

>MS.gene49436

ATGGATAGATTGGTGGCTATGTTAGCAGGATTCTCTTTACCAAATGGTTGCAAAAGAACCTGTCTGGTTGCTGTTATTGGCAATTCACTTGATCAAACTTTCTTATTTGGAAGTGAAGACTTCAAGATTGCGGGTTTTGATGGGAAATTAAGCCTCAACAAGGGATATGTGGCTGGCATATTAACAGTAGATACTGTTGCAGACTTTCTACCTAGAAAGGGGCCTCTGCGACAGAGAAGGTGAGTTAAAACTTGTTTCTGAATCTGGGTTTGTGAGTGAGGATTTATGACACGAATAATGATTGAAATGCAAAAACTGTGTATGAATAAACTTGAGACTAAAAATCATCTTTGAACGCAAAAACTACAATTTCTTGATACAAGTTAAAACTGTGTATGAATAAACTTGAGACTAATAAACTTGTTTTCCGCTTGCCTTTCATTCCTGTGTAATTTTCAAGTTATCTGTATTTGAAAATTTACCAAAATACAAACTACTTTTTATCTAGAGGCAAAATCAATTGTACTCCAAAACATGTTAAAATTAATTGTGTCTGAATTCGCTTTTGTCTTCCGGAGTCCAGACATGTCTCAACAATTTTCAGATAATTATGTTCTGTTATAAACATATAGACCTTAAATGCTTCTAGCTTGTCTTGTTTTAACTATCCACATACTCCCTCCAGTCTCGTGTATAAGAAAATGATAGAAGTTTTTTTCCTACCAATTATAAGAAAAAGATACAATTTAAGATTTATTAATTAATTATTTTACTTTTATACCCTTATTTTGTCTTTTAATGCATCTATTCCATATAAACAGTAGGTGTTACCATCCTCGAAGAGCATTTAATTTAGAGTCATGTTTGGGATAAACTATCAAAATTATAGCATTAAGTGGTCTCATCTTCTTGATGATTGTTTTTTTCTTTGACCAGGGACATTTATCACATAACTTGTCTTTCATTTGTGTTTTGCTGTAAAAGTAACTAAGTCCATGTTCTTTCATCGCATGTCAATAGTTCTTCGTGGTTAGTTTGTAGCCTTGCACTTGTTTAAAACAGAAGTTAAGATCTCTTCATATTGATCACTGAGGCAAGAATGGACTTTTGGTTAATCGAAACATGCTAAAAATCTTTTATGAAATTCATTGATTCCTGCATTACCCTTCAAAATGGTAAACTATGTTTCTAACTATGTATAAGGCGATGTTAGATTATGTATTTATGTTCCTAGTTTACTCATGTTACTAGTGAATCTTTGCTTATAGCTGCAACTTTTTATGTATAAAAACTATTGTAGGACTGGAATTGCATACATATCAAATGTGGCAGTGAGAGAAAAATTTAGACAGAAAGGAATAGCTAAACAATTGGTGGCTAAGGCAGAATCACAAGCCAGAAGTTGGGGATGTCGTGCCATTGCATTACACTGCGATTTACGAAATCCTGCGGCAACAAAGTTATACCAAGGGCAAGGTTTCAAATCTATAAAGGTTCCGGGAGGAGCTAACTGGCCTCAACCAAAAACTTCTCCAGATGTGAAGTTCAACTTCATGATGAAGCTTCTCAACAAATCAGCAGTTTCTTAA

Protein sequence

>MS.gene49436.t1

MDRLVAMLAGFSLPNGCKRTCLVAVIGNSLDQTFLFGSEDFKIAGFDGKLSLNKGYVAGILTVDTVADFLPRKGPLRQRRTGIAYISNVAVREKFRQKGIAKQLVAKAESQARSWGCRAIALHCDLRNPAATKLYQGQGFKSIKVPGGANWPQPKTSPDVKFNFMMKLLNKSAVS