Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074519.t1 | XP_013451320.1 | 92.8 | 456 | 31 | 1 | 2 | 457 | 1 | 454 | 1.20E-65 | 260.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074519.t1 | A0A072UHW8 | 92.8 | 456 | 31 | 1 | 2 | 457 | 1 | 454 | 8.7e-66 | 260.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene074519 | 0.848828 | 4.78E-60 | -1.69E-46 |
| MS.gene049350 | MS.gene074519 | 0.862227 | 5.88E-64 | -1.69E-46 |
| MS.gene049711 | MS.gene074519 | 0.843389 | 1.44E-58 | -1.69E-46 |
| MS.gene049807 | MS.gene074519 | 0.830517 | 2.81E-55 | -1.69E-46 |
| MS.gene049821 | MS.gene074519 | 0.832414 | 9.58E-56 | -1.69E-46 |
| MS.gene049824 | MS.gene074519 | 0.810065 | 1.39E-50 | -1.69E-46 |
| MS.gene049825 | MS.gene074519 | 0.806832 | 6.79E-50 | -1.69E-46 |
| MS.gene049850 | MS.gene074519 | 0.847508 | 1.11E-59 | -1.69E-46 |
| MS.gene049887 | MS.gene074519 | 0.825722 | 4.01E-54 | -1.69E-46 |
| MS.gene050807 | MS.gene074519 | 0.824432 | 8.10E-54 | -1.69E-46 |
| MS.gene050808 | MS.gene074519 | 0.811328 | 7.40E-51 | -1.69E-46 |
| MS.gene050838 | MS.gene074519 | 0.806837 | 6.77E-50 | -1.69E-46 |
| MS.gene050901 | MS.gene074519 | 0.841625 | 4.24E-58 | -1.69E-46 |
| MS.gene051018 | MS.gene074519 | 0.829046 | 6.41E-55 | -1.69E-46 |
| MS.gene051021 | MS.gene074519 | 0.826647 | 2.42E-54 | -1.69E-46 |
| MS.gene051029 | MS.gene074519 | 0.807332 | 5.32E-50 | -1.69E-46 |
| MS.gene051247 | MS.gene074519 | 0.838204 | 3.30E-57 | -1.69E-46 |
| MS.gene051279 | MS.gene074519 | 0.820572 | 6.39E-53 | -1.69E-46 |
| MS.gene051388 | MS.gene074519 | 0.813532 | 2.44E-51 | -1.69E-46 |
| MS.gene05157 | MS.gene074519 | 0.833717 | 4.54E-56 | -1.69E-46 |
| MS.gene051664 | MS.gene074519 | 0.822577 | 2.20E-53 | -1.69E-46 |
| MS.gene051700 | MS.gene074519 | 0.843223 | 1.60E-58 | -1.69E-46 |
| MS.gene051719 | MS.gene074519 | 0.894717 | 1.90E-75 | -1.69E-46 |
| MS.gene051720 | MS.gene074519 | 0.89975 | 1.45E-77 | -1.69E-46 |
| MS.gene051744 | MS.gene074519 | 0.810408 | 1.17E-50 | -1.69E-46 |
| MS.gene051885 | MS.gene074519 | 0.801889 | 7.28E-49 | -1.69E-46 |
| MS.gene051957 | MS.gene074519 | 0.800929 | 1.14E-48 | -1.69E-46 |
| MS.gene052000 | MS.gene074519 | 0.804772 | 1.84E-49 | -1.69E-46 |
| MS.gene052133 | MS.gene074519 | 0.840657 | 7.62E-58 | -1.69E-46 |
| MS.gene052335 | MS.gene074519 | 0.817148 | 3.83E-52 | -1.69E-46 |
| MS.gene052463 | MS.gene074519 | 0.827376 | 1.62E-54 | -1.69E-46 |
| MS.gene052516 | MS.gene074519 | 0.857015 | 2.18E-62 | -1.69E-46 |
| MS.gene052519 | MS.gene074519 | 0.803456 | 3.46E-49 | -1.69E-46 |
| MS.gene052665 | MS.gene074519 | 0.82717 | 1.81E-54 | -1.69E-46 |
| MS.gene052673 | MS.gene074519 | -0.824102 | 9.68E-54 | -1.69E-46 |
| MS.gene052732 | MS.gene074519 | 0.843356 | 1.47E-58 | -1.69E-46 |
| MS.gene052773 | MS.gene074519 | 0.802391 | 5.74E-49 | -1.69E-46 |
| MS.gene052863 | MS.gene074519 | 0.803508 | 3.37E-49 | -1.69E-46 |
| MS.gene052896 | MS.gene074519 | 0.80179 | 7.63E-49 | -1.69E-46 |
| MS.gene053079 | MS.gene074519 | 0.811377 | 7.22E-51 | -1.69E-46 |
| MS.gene053122 | MS.gene074519 | 0.826134 | 3.20E-54 | -1.69E-46 |
| MS.gene053144 | MS.gene074519 | 0.881467 | 2.34E-70 | -1.69E-46 |
| MS.gene053164 | MS.gene074519 | 0.809817 | 1.57E-50 | -1.69E-46 |
| MS.gene053171 | MS.gene074519 | 0.83174 | 1.41E-55 | -1.69E-46 |
| MS.gene053174 | MS.gene074519 | 0.806987 | 6.30E-50 | -1.69E-46 |
| MS.gene053226 | MS.gene074519 | 0.835746 | 1.40E-56 | -1.69E-46 |
| MS.gene053536 | MS.gene074519 | 0.828998 | 6.58E-55 | -1.69E-46 |
| MS.gene053538 | MS.gene074519 | 0.830499 | 2.84E-55 | -1.69E-46 |
| MS.gene053581 | MS.gene074519 | 0.88268 | 8.51E-71 | -1.69E-46 |
| MS.gene053599 | MS.gene074519 | 0.833926 | 4.03E-56 | -1.69E-46 |
| MS.gene05364 | MS.gene074519 | 0.810416 | 1.17E-50 | -1.69E-46 |
| MS.gene05423 | MS.gene074519 | 0.831369 | 1.74E-55 | -1.69E-46 |
| MS.gene054584 | MS.gene074519 | 0.873281 | 1.66E-67 | -1.69E-46 |
| MS.gene05458 | MS.gene074519 | 0.831207 | 1.90E-55 | -1.69E-46 |
| MS.gene054651 | MS.gene074519 | 0.820213 | 7.72E-53 | -1.69E-46 |
| MS.gene054775 | MS.gene074519 | 0.800805 | 1.21E-48 | -1.69E-46 |
| MS.gene054991 | MS.gene074519 | 0.819013 | 1.45E-52 | -1.69E-46 |
| MS.gene055083 | MS.gene074519 | 0.817641 | 2.97E-52 | -1.69E-46 |
| MS.gene055144 | MS.gene074519 | 0.842505 | 2.48E-58 | -1.69E-46 |
| MS.gene055465 | MS.gene074519 | 0.903756 | 2.50E-79 | -1.69E-46 |
| MS.gene055466 | MS.gene074519 | 0.891631 | 3.33E-74 | -1.69E-46 |
| MS.gene055467 | MS.gene074519 | 0.822526 | 2.26E-53 | -1.69E-46 |
| MS.gene055468 | MS.gene074519 | 0.894013 | 3.68E-75 | -1.69E-46 |
| MS.gene055469 | MS.gene074519 | 0.875984 | 2.00E-68 | -1.69E-46 |
| MS.gene055512 | MS.gene074519 | 0.803115 | 4.07E-49 | -1.69E-46 |
| MS.gene055513 | MS.gene074519 | 0.831271 | 1.84E-55 | -1.69E-46 |
| MS.gene055537 | MS.gene074519 | 0.878043 | 3.86E-69 | -1.69E-46 |
| MS.gene055580 | MS.gene074519 | -0.884053 | 2.66E-71 | -1.69E-46 |
| MS.gene055764 | MS.gene074519 | 0.817265 | 3.61E-52 | -1.69E-46 |
| MS.gene055778 | MS.gene074519 | 0.800636 | 1.31E-48 | -1.69E-46 |
| MS.gene055861 | MS.gene074519 | -0.805966 | 1.03E-49 | -1.69E-46 |
| MS.gene055890 | MS.gene074519 | 0.814684 | 1.36E-51 | -1.69E-46 |
| MS.gene055891 | MS.gene074519 | 0.858112 | 1.03E-62 | -1.69E-46 |
| MS.gene056005 | MS.gene074519 | 0.826621 | 2.45E-54 | -1.69E-46 |
| MS.gene056043 | MS.gene074519 | -0.869019 | 4.23E-66 | -1.69E-46 |
| MS.gene056215 | MS.gene074519 | 0.812761 | 3.60E-51 | -1.69E-46 |
| MS.gene056308 | MS.gene074519 | 0.844056 | 9.57E-59 | -1.69E-46 |
| MS.gene056507 | MS.gene074519 | 0.803645 | 3.16E-49 | -1.69E-46 |
| MS.gene056595 | MS.gene074519 | 0.804597 | 2.00E-49 | -1.69E-46 |
| MS.gene057294 | MS.gene074519 | 0.862373 | 5.31E-64 | -1.69E-46 |
| MS.gene057915 | MS.gene074519 | 0.818144 | 2.29E-52 | -1.69E-46 |
| MS.gene058087 | MS.gene074519 | 0.86562 | 5.17E-65 | -1.69E-46 |
| MS.gene058339 | MS.gene074519 | 0.84689 | 1.63E-59 | -1.69E-46 |
| MS.gene058492 | MS.gene074519 | 0.83313 | 6.36E-56 | -1.69E-46 |
| MS.gene058625 | MS.gene074519 | 0.843358 | 1.47E-58 | -1.69E-46 |
| MS.gene058668 | MS.gene074519 | 0.833035 | 6.72E-56 | -1.69E-46 |
| MS.gene058852 | MS.gene074519 | 0.839906 | 1.20E-57 | -1.69E-46 |
| MS.gene058945 | MS.gene074519 | 0.838796 | 2.32E-57 | -1.69E-46 |
| MS.gene05894 | MS.gene074519 | 0.817802 | 2.73E-52 | -1.69E-46 |
| MS.gene059015 | MS.gene074519 | 0.800638 | 1.31E-48 | -1.69E-46 |
| MS.gene059084 | MS.gene074519 | 0.819944 | 8.90E-53 | -1.69E-46 |
| MS.gene059170 | MS.gene074519 | 0.80723 | 5.59E-50 | -1.69E-46 |
| MS.gene059424 | MS.gene074519 | 0.816116 | 6.53E-52 | -1.69E-46 |
| MS.gene059426 | MS.gene074519 | 0.810145 | 1.33E-50 | -1.69E-46 |
| MS.gene059570 | MS.gene074519 | 0.804423 | 2.18E-49 | -1.69E-46 |
| MS.gene059625 | MS.gene074519 | 0.825638 | 4.20E-54 | -1.69E-46 |
| MS.gene059725 | MS.gene074519 | 0.805693 | 1.18E-49 | -1.69E-46 |
| MS.gene060429 | MS.gene074519 | 0.803876 | 2.83E-49 | -1.69E-46 |
| MS.gene060519 | MS.gene074519 | 0.876682 | 1.15E-68 | -1.69E-46 |
| MS.gene060587 | MS.gene074519 | 0.867093 | 1.76E-65 | -1.69E-46 |
| MS.gene060884 | MS.gene074519 | 0.813249 | 2.82E-51 | -1.69E-46 |
| MS.gene061006 | MS.gene074519 | 0.809189 | 2.14E-50 | -1.69E-46 |
| MS.gene061100 | MS.gene074519 | 0.827337 | 1.65E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074519.t1 | MTR_6g018780 | 92.763 | 456 | 31 | 1 | 2 | 457 | 1 | 454 | 0.0 | 851 |
| MS.gene074519.t1 | MTR_7g082230 | 52.143 | 140 | 56 | 5 | 315 | 450 | 45 | 177 | 7.71e-31 | 117 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074519.t1 | AT5G38300 | 42.568 | 148 | 65 | 7 | 319 | 457 | 126 | 262 | 2.57e-16 | 79.3 |
| MS.gene074519.t1 | AT1G67035 | 48.039 | 102 | 34 | 5 | 340 | 435 | 107 | 195 | 2.39e-14 | 72.8 |
Find 93 sgRNAs with CRISPR-Local
Find 134 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTCAACCAAATTCCTTTC+TGG | 0.113204 | 6.1:+4792388 | None:intergenic |
| GTTAACCTCTTTCCTTGTTT+TGG | 0.201042 | 6.1:+4791904 | None:intergenic |
| TTGAATTTGAATTTGAATTT+TGG | 0.204549 | 6.1:+4792578 | None:intergenic |
| TTTGAAGGTGATTTGGGTTA+TGG | 0.234233 | 6.1:-4792091 | MS.gene074519:CDS |
| TTAGTTCTGGATGCAAATTT+TGG | 0.235426 | 6.1:+4792127 | None:intergenic |
| CTTAACCTCTTTCTTAGTTC+TGG | 0.237039 | 6.1:+4792114 | None:intergenic |
| TGGTTAAGGATCAATGAAAT+TGG | 0.265606 | 6.1:+4792598 | None:intergenic |
| CTTGTTTCTTGTGGGAGAAA+TGG | 0.269447 | 6.1:-4792187 | MS.gene074519:CDS |
| GATAACTTTGAAGGTGGATT+TGG | 0.272280 | 6.1:-4792268 | MS.gene074519:CDS |
| TGTTGACCCAGAAAGGAATT+TGG | 0.281401 | 6.1:-4792395 | MS.gene074519:CDS |
| GTTAACTTTCATGGTGGATT+TGG | 0.284316 | 6.1:-4791887 | MS.gene074519:CDS |
| CAAACCAACCTTGTCCTCTA+AGG | 0.295035 | 6.1:-4792558 | MS.gene074519:CDS |
| TGAAGTAAAGGTTCAAGAAT+TGG | 0.296365 | 6.1:+4792631 | None:intergenic |
| TTTGATGGTGATTTGGGTTA+TGG | 0.298121 | 6.1:-4792367 | MS.gene074519:CDS |
| TTGAAGGTGATTTGGGTTAT+GGG | 0.301077 | 6.1:-4792090 | MS.gene074519:CDS |
| GGTTAAGTTTGAAGGTGATT+TGG | 0.308687 | 6.1:-4792098 | MS.gene074519:CDS |
| TCTAAAGAAGTAAGCCTTAG+AGG | 0.309661 | 6.1:+4792544 | None:intergenic |
| ACTGGAACAAGAAGAAAATT+TGG | 0.311441 | 6.1:-4791846 | MS.gene074519:CDS |
| GTTAAGTTTGAAGGTGATTT+GGG | 0.317289 | 6.1:-4792097 | MS.gene074519:CDS |
| GGTTGAGTTTGATGGTGATT+TGG | 0.317896 | 6.1:-4792374 | MS.gene074519:CDS |
| TTATGGGAGAAAAGGTTCTA+TGG | 0.319613 | 6.1:-4791759 | MS.gene074519:CDS |
| GGGAAAAGAGGTTGCAGTTA+AGG | 0.328889 | 6.1:-4792468 | MS.gene074519:CDS |
| TTCATGGTGGATTTGGTTAC+GGG | 0.336325 | 6.1:-4791880 | MS.gene074519:CDS |
| GTTGAGTTTGATGGTGATTT+GGG | 0.338151 | 6.1:-4792373 | MS.gene074519:CDS |
| TTGTGGGAGAAATGGGTCTT+TGG | 0.351667 | 6.1:-4792179 | MS.gene074519:CDS |
| AGGAAAGAGGTTAACTTTCA+TGG | 0.357231 | 6.1:-4791896 | MS.gene074519:CDS |
| TTGATGGTGATTTGGGTTAT+GGG | 0.366339 | 6.1:-4792366 | MS.gene074519:CDS |
| TTGTTTCTTGTGGGAGAAAT+GGG | 0.366891 | 6.1:-4792186 | MS.gene074519:CDS |
| ACTCAACCAAATTCCTTTCT+GGG | 0.386526 | 6.1:+4792389 | None:intergenic |
| GAAGTAAAGGTTCAAGAATT+GGG | 0.393264 | 6.1:+4792632 | None:intergenic |
| TTCAAGGTGGATTGGCTTGT+GGG | 0.394720 | 6.1:-4791673 | MS.gene074519:CDS |
| AAGAAAGAGGTTAAGTTTGA+AGG | 0.396584 | 6.1:-4792106 | MS.gene074519:CDS |
| CACCACTCCATTATCATCCT+TGG | 0.396729 | 6.1:+4791517 | None:intergenic |
| GATTGAATCAGAGAAAGTTT+TGG | 0.402844 | 6.1:-4792308 | MS.gene074519:CDS |
| GATTTGGGTTATGGGAGAAA+TGG | 0.411151 | 6.1:-4792358 | MS.gene074519:CDS |
| AGGAATTTGGTTGAGTTTGA+TGG | 0.415010 | 6.1:-4792382 | MS.gene074519:CDS |
| GGATTTGGTTACGGGAGAAA+AGG | 0.419540 | 6.1:-4791872 | MS.gene074519:CDS |
| AGAAAAGGTTCTTCTGTTGT+TGG | 0.425298 | 6.1:-4791650 | MS.gene074519:CDS |
| TTTCATGGTGGATTTGGTTA+CGG | 0.427010 | 6.1:-4791881 | MS.gene074519:CDS |
| GGATTTGGTTATGAGAGAAA+AGG | 0.430577 | 6.1:-4792253 | MS.gene074519:CDS |
| TTATGAGAGAAAAGGGTCAT+TGG | 0.434058 | 6.1:-4792245 | MS.gene074519:CDS |
| AGGAAAGAGGTTAGGTTTCA+AGG | 0.439962 | 6.1:-4791791 | MS.gene074519:CDS |
| ATTTGGGTTATGGGAGAAAT+GGG | 0.443961 | 6.1:-4792357 | MS.gene074519:CDS |
| AAGAAAACTGTTGGCGGCGG+CGG | 0.447845 | 6.1:-4791446 | MS.gene074519:CDS |
| GGCTAAGTTTCAAGGTGGAT+TGG | 0.451767 | 6.1:-4791681 | MS.gene074519:CDS |
| CCTCAACAAGGAAAGAGGTT+AGG | 0.463514 | 6.1:-4791799 | MS.gene074519:CDS |
| GATTTGGGTTATGGGAGAAA+AGG | 0.464132 | 6.1:-4792082 | MS.gene074519:CDS |
| TTATGGGAGAAAAGGGTCAT+TGG | 0.475958 | 6.1:-4792074 | MS.gene074519:CDS |
| GGATTGGCTTGTGGGAGAAA+AGG | 0.477021 | 6.1:-4791665 | MS.gene074519:CDS |
| TGCATCCAAAACAAGGAAAG+AGG | 0.487662 | 6.1:-4791909 | MS.gene074519:CDS |
| GATTTGGTTATGAGAGAAAA+GGG | 0.490244 | 6.1:-4792252 | MS.gene074519:CDS |
| TTATGGGAGAAAAGGGTTGT+TGG | 0.492331 | 6.1:-4791969 | MS.gene074519:CDS |
| GGGAGTAAGTCAGAGAGTGC+AGG | 0.493403 | 6.1:-4791482 | MS.gene074519:CDS |
| TGCATCCAGAACTAAGAAAG+AGG | 0.496584 | 6.1:-4792119 | MS.gene074519:CDS |
| TGTAGCCTCAACAAGGAAAG+AGG | 0.497480 | 6.1:-4791804 | MS.gene074519:CDS |
| ATGATCGTTATGCTGAATTG+AGG | 0.497816 | 6.1:-4792489 | MS.gene074519:CDS |
| AGAAAAGAGGCTAAGTTTCA+AGG | 0.499190 | 6.1:-4791689 | MS.gene074519:CDS |
| CTGTATTGAGGAAAGAGAAT+CGG | 0.499795 | 6.1:-4791604 | MS.gene074519:CDS |
| ATTTGGGTTATGGGAGAAAA+GGG | 0.506933 | 6.1:-4792081 | MS.gene074519:CDS |
| GAATTATTTGCCTGCAGTGA+TGG | 0.512436 | 6.1:-4791573 | MS.gene074519:CDS |
| GATTTGGTTACGGGAGAAAA+GGG | 0.515820 | 6.1:-4791871 | MS.gene074519:CDS |
| GTCATTATCTCCATCACTGC+AGG | 0.516529 | 6.1:+4791563 | None:intergenic |
| AGGTGTTTGATGAATGTTCA+AGG | 0.520295 | 6.1:-4792448 | MS.gene074519:CDS |
| GCTATCAATGGTGAAGGAAG+AGG | 0.520988 | 6.1:-4791353 | MS.gene074519:CDS |
| TGATCGTTATGCTGAATTGA+GGG | 0.522928 | 6.1:-4792488 | MS.gene074519:CDS |
| CCTAACCTCTTTCCTTGTTG+AGG | 0.526122 | 6.1:+4791799 | None:intergenic |
| TTTCAAGGTGGATTGGCTTG+TGG | 0.527345 | 6.1:-4791674 | MS.gene074519:CDS |
| GGAGTAAGTCAGAGAGTGCA+GGG | 0.531282 | 6.1:-4791481 | MS.gene074519:CDS |
| ATGCTTGCATTGATGAGCTA+AGG | 0.533829 | 6.1:-4791400 | MS.gene074519:CDS |
| ATGCTGAATTGAGGGAAAAG+AGG | 0.541617 | 6.1:-4792480 | MS.gene074519:CDS |
| GGATCAAGGACATGAAGTAA+AGG | 0.555255 | 6.1:+4792619 | None:intergenic |
| GGATCAATGAAATTGGATCA+AGG | 0.555295 | 6.1:+4792605 | None:intergenic |
| TGCAACCAAAGCAAGAAAAG+AGG | 0.555909 | 6.1:-4791702 | MS.gene074519:CDS |
| GAAGTAAGCCTTAGAGGACA+AGG | 0.558378 | 6.1:+4792550 | None:intergenic |
| ACTGCAGGCAAATAATTCAA+TGG | 0.561302 | 6.1:+4791578 | None:intergenic |
| AAGAATAAGAAGAAAACTGT+TGG | 0.563642 | 6.1:-4791455 | MS.gene074519:CDS |
| GTTGAGGCTACAAATTTCTG+TGG | 0.573758 | 6.1:+4791815 | None:intergenic |
| GCTACTGCAACTGCTATCAA+TGG | 0.576687 | 6.1:-4791365 | MS.gene074519:CDS |
| TTCCAAGGATGATAATGGAG+TGG | 0.592140 | 6.1:-4791519 | MS.gene074519:CDS |
| TAAGCCTTAGAGGACAAGGT+TGG | 0.592914 | 6.1:+4792554 | None:intergenic |
| ATGGTGAAGGAAGAGGAGGA+AGG | 0.593712 | 6.1:-4791346 | MS.gene074519:CDS |
| TTACGGGAGAAAAGGGTCAC+TGG | 0.594430 | 6.1:-4791864 | MS.gene074519:CDS |
| GCAACTGCTATCAATGGTGA+AGG | 0.594488 | 6.1:-4791359 | MS.gene074519:CDS |
| AAAGAGGTTAGGTTTCAAGG+TGG | 0.599875 | 6.1:-4791788 | MS.gene074519:CDS |
| AATAAGAAGAAAACTGTTGG+CGG | 0.607019 | 6.1:-4791452 | MS.gene074519:CDS |
| AGAAATTTGTAGCCTCAACA+AGG | 0.608034 | 6.1:-4791811 | MS.gene074519:CDS |
| ATCAATGGTGAAGGAAGAGG+AGG | 0.624042 | 6.1:-4791350 | MS.gene074519:CDS |
| GGAGTGGTGTCAAATTCAAG+AGG | 0.628865 | 6.1:-4791503 | MS.gene074519:CDS |
| AAGAAGAAAACTGTTGGCGG+CGG | 0.632313 | 6.1:-4791449 | MS.gene074519:CDS |
| AAAGAGGTTAACTTTCATGG+TGG | 0.633453 | 6.1:-4791893 | MS.gene074519:CDS |
| GAGTAAGTCAGAGAGTGCAG+GGG | 0.654132 | 6.1:-4791480 | MS.gene074519:CDS |
| AAAGAGGCTAAGTTTCAAGG+TGG | 0.660328 | 6.1:-4791686 | MS.gene074519:CDS |
| GAGTGGTGTCAAATTCAAGA+GGG | 0.698354 | 6.1:-4791502 | MS.gene074519:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTGAATTTGAATTTGAATTT+TGG | + | chr6.1:4791372-4791391 | None:intergenic | 15.0% |
| !!! | AAAAGAAGAAGATTTTGATA+TGG | - | chr6.1:4791897-4791916 | MS.gene074519:CDS | 20.0% |
| !!! | AAAAGAAGAATTTTTTGAGA+TGG | - | chr6.1:4791792-4791811 | MS.gene074519:CDS | 20.0% |
| !!! | TTGAATTTGAATTTTGGTTA+AGG | + | chr6.1:4791366-4791385 | None:intergenic | 20.0% |
| !!! | TTTAGAAGATAGTTTTGTTA+TGG | - | chr6.1:4791420-4791439 | MS.gene074519:CDS | 20.0% |
| ! | AAAAATTTGCATCCAAAACA+AGG | - | chr6.1:4792031-4792050 | MS.gene074519:CDS | 25.0% |
| ! | AAGAAAAAAGTGAACTTTGA+AGG | - | chr6.1:4791946-4791965 | MS.gene074519:CDS | 25.0% |
| ! | AAGAATAAGAAGAAAACTGT+TGG | - | chr6.1:4792492-4792511 | MS.gene074519:CDS | 25.0% |
| ! | AGGAAAAATGATAACTTTGA+AGG | - | chr6.1:4791670-4791689 | MS.gene074519:CDS | 25.0% |
| !! | AAAAATGATAACTTTGAAGG+TGG | - | chr6.1:4791673-4791692 | MS.gene074519:CDS | 25.0% |
| !! | TTCAAAAACTTGTTTCTTGT+GGG | - | chr6.1:4791752-4791771 | MS.gene074519:CDS | 25.0% |
| !! | TTTCAAAAACTTGTTTCTTG+TGG | - | chr6.1:4791751-4791770 | MS.gene074519:CDS | 25.0% |
| !!! | AAGTTATCATTTTTCCTTGA+AGG | + | chr6.1:4791667-4791686 | None:intergenic | 25.0% |
| !!! | ACAAGATGAATATTTTGAGA+TGG | - | chr6.1:4792002-4792021 | MS.gene074519:CDS | 25.0% |
| !!! | AGTTATCATTTTTCCTTGAA+GGG | + | chr6.1:4791666-4791685 | None:intergenic | 25.0% |
| AAGAAAGAGGTTAAGTTTGA+AGG | - | chr6.1:4791841-4791860 | MS.gene074519:CDS | 30.0% | |
| AATAAGAAGAAAACTGTTGG+CGG | - | chr6.1:4792495-4792514 | MS.gene074519:CDS | 30.0% | |
| ACTGGAACAAGAAGAAAATT+TGG | - | chr6.1:4792101-4792120 | MS.gene074519:CDS | 30.0% | |
| GAAGTAAAGGTTCAAGAATT+GGG | + | chr6.1:4791318-4791337 | None:intergenic | 30.0% | |
| GTTAAGTTTGAAGGTGATTT+GGG | - | chr6.1:4791850-4791869 | MS.gene074519:CDS | 30.0% | |
| TGAAGTAAAGGTTCAAGAAT+TGG | + | chr6.1:4791319-4791338 | None:intergenic | 30.0% | |
| TGGTTAAGGATCAATGAAAT+TGG | + | chr6.1:4791352-4791371 | None:intergenic | 30.0% | |
| ! | AAAAAAGTGAACTTTGAAGG+TGG | - | chr6.1:4791949-4791968 | MS.gene074519:CDS | 30.0% |
| ! | AGTTTTTCCAAGGATGATAA+TGG | - | chr6.1:4792423-4792442 | MS.gene074519:CDS | 30.0% |
| ! | GATTGAATCAGAGAAAGTTT+TGG | - | chr6.1:4791639-4791658 | MS.gene074519:CDS | 30.0% |
| ! | GATTTGGTTATGAGAGAAAA+GGG | - | chr6.1:4791695-4791714 | MS.gene074519:CDS | 30.0% |
| !! | GTTATCATTTTTCCTTGAAG+GGG | + | chr6.1:4791665-4791684 | None:intergenic | 30.0% |
| !! | TTCAAGGTGGATTTTCTTAT+GGG | - | chr6.1:4792172-4792191 | MS.gene074519:CDS | 30.0% |
| !! | TTTCAAGGTGGATTTTCTTA+TGG | - | chr6.1:4792171-4792190 | MS.gene074519:CDS | 30.0% |
| !!! | TTAGTTCTGGATGCAAATTT+TGG | + | chr6.1:4791823-4791842 | None:intergenic | 30.0% |
| AAAAAGTTGTTGACCCAGAA+AGG | - | chr6.1:4791545-4791564 | MS.gene074519:CDS | 35.0% | |
| AAAGAGGTTAACTTTCATGG+TGG | - | chr6.1:4792054-4792073 | MS.gene074519:CDS | 35.0% | |
| AACTCAACCAAATTCCTTTC+TGG | + | chr6.1:4791562-4791581 | None:intergenic | 35.0% | |
| ACTCAACCAAATTCCTTTCT+GGG | + | chr6.1:4791561-4791580 | None:intergenic | 35.0% | |
| ACTGCAGGCAAATAATTCAA+TGG | + | chr6.1:4792372-4792391 | None:intergenic | 35.0% | |
| AGAAAAGAGGCTAAGTTTCA+AGG | - | chr6.1:4792258-4792277 | MS.gene074519:CDS | 35.0% | |
| AGAAAAGGTTCTTCTGTTGT+TGG | - | chr6.1:4792297-4792316 | MS.gene074519:CDS | 35.0% | |
| AGAAATTTGTAGCCTCAACA+AGG | - | chr6.1:4792136-4792155 | MS.gene074519:CDS | 35.0% | |
| AGGAAAGAGGTTAACTTTCA+TGG | - | chr6.1:4792051-4792070 | MS.gene074519:CDS | 35.0% | |
| AGGAATTTGGTTGAGTTTGA+TGG | - | chr6.1:4791565-4791584 | MS.gene074519:CDS | 35.0% | |
| AGGTGTTTGATGAATGTTCA+AGG | - | chr6.1:4791499-4791518 | MS.gene074519:CDS | 35.0% | |
| ATCCTTGGAAAAACTACTCT+TGG | + | chr6.1:4792418-4792437 | None:intergenic | 35.0% | |
| ATGATCGTTATGCTGAATTG+AGG | - | chr6.1:4791458-4791477 | MS.gene074519:CDS | 35.0% | |
| CTCAATACAGCAGAAAAATC+AGG | + | chr6.1:4792333-4792352 | None:intergenic | 35.0% | |
| CTGTATTGAGGAAAGAGAAT+CGG | - | chr6.1:4792343-4792362 | MS.gene074519:CDS | 35.0% | |
| CTTAACCTCTTTCTTAGTTC+TGG | + | chr6.1:4791836-4791855 | None:intergenic | 35.0% | |
| GGATCAATGAAATTGGATCA+AGG | + | chr6.1:4791345-4791364 | None:intergenic | 35.0% | |
| GGTTAAGTTTGAAGGTGATT+TGG | - | chr6.1:4791849-4791868 | MS.gene074519:CDS | 35.0% | |
| TCTAAAGAAGTAAGCCTTAG+AGG | + | chr6.1:4791406-4791425 | None:intergenic | 35.0% | |
| TGATCGTTATGCTGAATTGA+GGG | - | chr6.1:4791459-4791478 | MS.gene074519:CDS | 35.0% | |
| TTATGAGAGAAAAGGGTCAT+TGG | - | chr6.1:4791702-4791721 | MS.gene074519:CDS | 35.0% | |
| TTATGGGAGAAAAGGTTCTA+TGG | - | chr6.1:4792188-4792207 | MS.gene074519:CDS | 35.0% | |
| TTGGAAAAACTACTCTTGGA+AGG | + | chr6.1:4792414-4792433 | None:intergenic | 35.0% | |
| ! | ATTTGGGTTATGGGAGAAAA+GGG | - | chr6.1:4791866-4791885 | MS.gene074519:CDS | 35.0% |
| ! | ATTTGGGTTATGGGAGAAAT+GGG | - | chr6.1:4791590-4791609 | MS.gene074519:CDS | 35.0% |
| ! | CTGATTTTTCTGCTGTATTG+AGG | - | chr6.1:4792331-4792350 | MS.gene074519:CDS | 35.0% |
| ! | GAAGGAATAGTAGAGTTTTG+AGG | - | chr6.1:4792619-4792638 | MS.gene074519:CDS | 35.0% |
| ! | GATAACTTTGAAGGTGGATT+TGG | - | chr6.1:4791679-4791698 | MS.gene074519:CDS | 35.0% |
| ! | GGATTTGGTTATGAGAGAAA+AGG | - | chr6.1:4791694-4791713 | MS.gene074519:CDS | 35.0% |
| ! | GGATTTTCTTATGGGAGAAA+AGG | - | chr6.1:4792180-4792199 | MS.gene074519:CDS | 35.0% |
| ! | GTTTTGCTTATGGGAGAAAA+GGG | - | chr6.1:4791971-4791990 | MS.gene074519:CDS | 35.0% |
| ! | TTCCAAGAGTAGTTTTTCCA+AGG | - | chr6.1:4792413-4792432 | MS.gene074519:CDS | 35.0% |
| ! | TTGAAGGTGATTTGGGTTAT+GGG | - | chr6.1:4791857-4791876 | MS.gene074519:CDS | 35.0% |
| ! | TTGTTTCTTGTGGGAGAAAT+GGG | - | chr6.1:4791761-4791780 | MS.gene074519:CDS | 35.0% |
| ! | TTTGAAGGTGATTTGGGTTA+TGG | - | chr6.1:4791856-4791875 | MS.gene074519:CDS | 35.0% |
| !! | GTTAACCTCTTTCCTTGTTT+TGG | + | chr6.1:4792046-4792065 | None:intergenic | 35.0% |
| !! | GTTAACTTTCATGGTGGATT+TGG | - | chr6.1:4792060-4792079 | MS.gene074519:CDS | 35.0% |
| !! | GTTGAGTTTGATGGTGATTT+GGG | - | chr6.1:4791574-4791593 | MS.gene074519:CDS | 35.0% |
| !! | TTGAAGGTGGTTTTGCTTAT+GGG | - | chr6.1:4791962-4791981 | MS.gene074519:CDS | 35.0% |
| !! | TTGATGGTGATTTGGGTTAT+GGG | - | chr6.1:4791581-4791600 | MS.gene074519:CDS | 35.0% |
| !! | TTTCATGGTGGATTTGGTTA+CGG | - | chr6.1:4792066-4792085 | MS.gene074519:CDS | 35.0% |
| !! | TTTGAAGGTGGTTTTGCTTA+TGG | - | chr6.1:4791961-4791980 | MS.gene074519:CDS | 35.0% |
| !! | TTTGATGGTGATTTGGGTTA+TGG | - | chr6.1:4791580-4791599 | MS.gene074519:CDS | 35.0% |
| !!! | GATAGTTTTGTTATGGAGAG+AGG | - | chr6.1:4791427-4791446 | MS.gene074519:CDS | 35.0% |
| !!! | GTTCACTTTTTTCTTTGCTG+AGG | + | chr6.1:4791941-4791960 | None:intergenic | 35.0% |
| AAAGAGGCTAAGTTTCAAGG+TGG | - | chr6.1:4792261-4792280 | MS.gene074519:CDS | 40.0% | |
| AAAGAGGTTAGGTTTCAAGG+TGG | - | chr6.1:4792159-4792178 | MS.gene074519:CDS | 40.0% | |
| AGGAAAGAGGTTAGGTTTCA+AGG | - | chr6.1:4792156-4792175 | MS.gene074519:CDS | 40.0% | |
| ATGCTGAATTGAGGGAAAAG+AGG | - | chr6.1:4791467-4791486 | MS.gene074519:CDS | 40.0% | |
| GAAAAACTACTCTTGGAAGG+TGG | + | chr6.1:4792411-4792430 | None:intergenic | 40.0% | |
| GAATTATTTGCCTGCAGTGA+TGG | - | chr6.1:4792374-4792393 | MS.gene074519:CDS | 40.0% | |
| GGATCAAGGACATGAAGTAA+AGG | + | chr6.1:4791331-4791350 | None:intergenic | 40.0% | |
| GTTGAGGCTACAAATTTCTG+TGG | + | chr6.1:4792135-4792154 | None:intergenic | 40.0% | |
| TGCAACCAAAGCAAGAAAAG+AGG | - | chr6.1:4792245-4792264 | MS.gene074519:CDS | 40.0% | |
| TGCATCCAAAACAAGGAAAG+AGG | - | chr6.1:4792038-4792057 | MS.gene074519:CDS | 40.0% | |
| TGCATCCAGAACTAAGAAAG+AGG | - | chr6.1:4791828-4791847 | MS.gene074519:CDS | 40.0% | |
| TGTTGACCCAGAAAGGAATT+TGG | - | chr6.1:4791552-4791571 | MS.gene074519:CDS | 40.0% | |
| TTATGGGAGAAAAGGGTCAT+TGG | - | chr6.1:4791873-4791892 | MS.gene074519:CDS | 40.0% | |
| TTATGGGAGAAAAGGGTTGT+TGG | - | chr6.1:4791978-4791997 | MS.gene074519:CDS | 40.0% | |
| ! | ATGCTTGCATTGATGAGCTA+AGG | - | chr6.1:4792547-4792566 | MS.gene074519:CDS | 40.0% |
| ! | CTTAGCCTCTTTTCTTGCTT+TGG | + | chr6.1:4792253-4792272 | None:intergenic | 40.0% |
| ! | CTTGTTTCTTGTGGGAGAAA+TGG | - | chr6.1:4791760-4791779 | MS.gene074519:CDS | 40.0% |
| ! | GAGTGGTGTCAAATTCAAGA+GGG | - | chr6.1:4792445-4792464 | MS.gene074519:CDS | 40.0% |
| ! | GATTTGGGTTATGGGAGAAA+AGG | - | chr6.1:4791589-4791608 | MS.gene074519:CDS | 40.0% |
| ! | GATTTGGGTTATGGGAGAAA+TGG | - | chr6.1:4791589-4791608 | MS.gene074519:CDS | 40.0% |
| ! | GATTTGGTTACGGGAGAAAA+GGG | - | chr6.1:4792076-4792095 | MS.gene074519:CDS | 40.0% |
| ! | GCTTTGGTTGCAAGTTTTTG+TGG | + | chr6.1:4792237-4792256 | None:intergenic | 40.0% |
| ! | GGTTTTGCTTATGGGAGAAA+AGG | - | chr6.1:4791970-4791989 | MS.gene074519:CDS | 40.0% |
| !! | GGTTGAGTTTGATGGTGATT+TGG | - | chr6.1:4791573-4791592 | MS.gene074519:CDS | 40.0% |
| !! | GTTCTGGATGCAAATTTTGG+TGG | + | chr6.1:4791820-4791839 | None:intergenic | 40.0% |
| !! | GTTTTGTTATGGAGAGAGGA+AGG | - | chr6.1:4791431-4791450 | MS.gene074519:CDS | 40.0% |
| !! | TTCATGGTGGATTTGGTTAC+GGG | - | chr6.1:4792067-4792086 | MS.gene074519:CDS | 40.0% |
| !! | TTCCAAGGATGATAATGGAG+TGG | - | chr6.1:4792428-4792447 | MS.gene074519:CDS | 40.0% |
| AAGAAGAAAACTGTTGGCGG+CGG | - | chr6.1:4792498-4792517 | MS.gene074519:CDS | 45.0% | |
| ATCAATGGTGAAGGAAGAGG+AGG | - | chr6.1:4792597-4792616 | MS.gene074519:CDS | 45.0% | |
| CAAACCAACCTTGTCCTCTA+AGG | - | chr6.1:4791389-4791408 | MS.gene074519:CDS | 45.0% | |
| CACCACTCCATTATCATCCT+TGG | + | chr6.1:4792433-4792452 | None:intergenic | 45.0% | |
| CCTAACCTCTTTCCTTGTTG+AGG | + | chr6.1:4792151-4792170 | None:intergenic | 45.0% | |
| CCTCAACAAGGAAAGAGGTT+AGG | - | chr6.1:4792148-4792167 | MS.gene074519:CDS | 45.0% | |
| GAAGTAAGCCTTAGAGGACA+AGG | + | chr6.1:4791400-4791419 | None:intergenic | 45.0% | |
| GCAACTGCTATCAATGGTGA+AGG | - | chr6.1:4792588-4792607 | MS.gene074519:CDS | 45.0% | |
| GCTACTGCAACTGCTATCAA+TGG | - | chr6.1:4792582-4792601 | MS.gene074519:CDS | 45.0% | |
| GCTATCAATGGTGAAGGAAG+AGG | - | chr6.1:4792594-4792613 | MS.gene074519:CDS | 45.0% | |
| GGCTAAGTTTCAAGGTGGAT+TGG | - | chr6.1:4792266-4792285 | MS.gene074519:CDS | 45.0% | |
| GGGAAAAGAGGTTGCAGTTA+AGG | - | chr6.1:4791479-4791498 | MS.gene074519:CDS | 45.0% | |
| GTCATTATCTCCATCACTGC+AGG | + | chr6.1:4792387-4792406 | None:intergenic | 45.0% | |
| TAAGCCTTAGAGGACAAGGT+TGG | + | chr6.1:4791396-4791415 | None:intergenic | 45.0% | |
| TGTAGCCTCAACAAGGAAAG+AGG | - | chr6.1:4792143-4792162 | MS.gene074519:CDS | 45.0% | |
| TTGTGGGAGAAATGGGTCTT+TGG | - | chr6.1:4791768-4791787 | MS.gene074519:CDS | 45.0% | |
| ! | GGAGTGGTGTCAAATTCAAG+AGG | - | chr6.1:4792444-4792463 | MS.gene074519:CDS | 45.0% |
| ! | GGATTTGGTTACGGGAGAAA+AGG | - | chr6.1:4792075-4792094 | MS.gene074519:CDS | 45.0% |
| !! | AGAAAGTTTTGGCCCCTTCA+AGG | - | chr6.1:4791650-4791669 | MS.gene074519:CDS | 45.0% |
| !! | TTCAAGGTGGATTGGCTTGT+GGG | - | chr6.1:4792274-4792293 | MS.gene074519:CDS | 45.0% |
| !! | TTTCAAGGTGGATTGGCTTG+TGG | - | chr6.1:4792273-4792292 | MS.gene074519:CDS | 45.0% |
| ATGGTGAAGGAAGAGGAGGA+AGG | - | chr6.1:4792601-4792620 | MS.gene074519:CDS | 50.0% | |
| TTACGGGAGAAAAGGGTCAC+TGG | - | chr6.1:4792083-4792102 | MS.gene074519:CDS | 50.0% | |
| ! | GAGTAAGTCAGAGAGTGCAG+GGG | - | chr6.1:4792467-4792486 | MS.gene074519:CDS | 50.0% |
| ! | GGAGTAAGTCAGAGAGTGCA+GGG | - | chr6.1:4792466-4792485 | MS.gene074519:CDS | 50.0% |
| !! | GGATTGGCTTGTGGGAGAAA+AGG | - | chr6.1:4792282-4792301 | MS.gene074519:CDS | 50.0% |
| AAGAAAACTGTTGGCGGCGG+CGG | - | chr6.1:4792501-4792520 | MS.gene074519:CDS | 55.0% | |
| ! | GGGAGTAAGTCAGAGAGTGC+AGG | - | chr6.1:4792465-4792484 | MS.gene074519:CDS | 55.0% |
| !! | GCGGCGGCGTTTTGATGGGA+AGG | - | chr6.1:4792517-4792536 | MS.gene074519:CDS | 65.0% |
| ! | TGGCGGCGGCGGCGTTTTGA+TGG | - | chr6.1:4792512-4792531 | MS.gene074519:CDS | 70.0% |
| !! | GGCGGCGGCGGCGTTTTGAT+GGG | - | chr6.1:4792513-4792532 | MS.gene074519:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 4791298 | 4792671 | 4791298 | ID=MS.gene074519 |
| chr6.1 | mRNA | 4791298 | 4792671 | 4791298 | ID=MS.gene074519.t1;Parent=MS.gene074519 |
| chr6.1 | exon | 4791298 | 4792671 | 4791298 | ID=MS.gene074519.t1.exon1;Parent=MS.gene074519.t1 |
| chr6.1 | CDS | 4791298 | 4792671 | 4791298 | ID=cds.MS.gene074519.t1;Parent=MS.gene074519.t1 |
| Gene Sequence |
| Protein sequence |
