AlfalfaGEDB | MS.gene056952

M. sativa cultivar XinJiangDaYe / MS.gene056952

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF00076.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056952.t1	RHN63866.1	95.7	70	3	0	1	70	1	70	3.30E-31	143.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056952.t1	Q9DED4	39.1	69	41	1	3	70	1	69	6.8e-09	60.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056952.t1	G7JU64	95.7	70	3	0	1	70	1	70	2.4e-31	143.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049435	MS.gene056952	0.804368	2.23E-49	-1.69E-46
MS.gene050442	MS.gene056952	0.810116	1.35E-50	-1.69E-46
MS.gene052333	MS.gene056952	0.802592	5.21E-49	-1.69E-46
MS.gene054467	MS.gene056952	0.815217	1.04E-51	-1.69E-46
MS.gene054559	MS.gene056952	0.807805	4.22E-50	-1.69E-46
MS.gene056173	MS.gene056952	0.808498	3.01E-50	-1.69E-46
MS.gene056952	MS.gene057869	0.811296	7.52E-51	-1.69E-46
MS.gene056952	MS.gene061333	0.804107	2.53E-49	-1.69E-46
MS.gene056952	MS.gene06697	0.808821	2.57E-50	-1.69E-46
MS.gene056952	MS.gene06705	0.814826	1.26E-51	-1.69E-46
MS.gene056952	MS.gene06922	0.807355	5.26E-50	-1.69E-46
MS.gene056952	MS.gene072677	0.809383	1.94E-50	-1.69E-46
MS.gene056952	MS.gene27571	0.80821	3.46E-50	-1.69E-46
MS.gene056952	MS.gene30930	0.82058	6.36E-53	-1.69E-46
MS.gene056952	MS.gene31283	0.820354	7.17E-53	-1.69E-46
MS.gene056952	MS.gene35408	0.80698	6.32E-50	-1.69E-46
MS.gene056952	MS.gene35967	0.800237	1.58E-48	-1.69E-46
MS.gene056952	MS.gene43973	0.810385	1.18E-50	-1.69E-46
MS.gene056952	MS.gene44461	0.801281	9.70E-49	-1.69E-46
MS.gene056952	MS.gene53586	0.815693	8.11E-52	-1.69E-46
MS.gene056952	MS.gene60204	0.806342	8.62E-50	-1.69E-46
MS.gene056952	MS.gene60206	0.806065	9.86E-50	-1.69E-46
MS.gene056952	MS.gene60903	0.803196	3.91E-49	-1.69E-46
MS.gene056952	MS.gene61139	0.800814	1.21E-48	-1.69E-46
MS.gene056952	MS.gene64249	0.818505	1.89E-52	-1.69E-46
MS.gene056952	MS.gene68333	0.800865	1.18E-48	-1.69E-46
MS.gene056952	MS.gene72268	0.827737	1.33E-54	-1.69E-46
MS.gene056952	MS.gene82879	0.800254	1.57E-48	-1.69E-46
MS.gene056952	MS.gene83042	0.802643	5.09E-49	-1.69E-46
MS.gene056952	MS.gene84326	0.80525	1.46E-49	-1.69E-46
MS.gene056952	MS.gene94342	0.80251	5.42E-49	-1.69E-46
MS.gene056952	MS.gene95255	0.823701	1.20E-53	-1.69E-46
MS.gene056952	MS.gene99887	0.802593	5.21E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056952.t1	MTR_4g113150	95.714	70	3	0	1	70	1	70	9.92e-42	140
MS.gene056952.t1	MTR_4g113150	95.714	70	3	0	1	70	1	70	4.88e-41	140
MS.gene056952.t1	MTR_4g070080	45.161	62	33	1	10	70	9	70	4.42e-12	57.0
MS.gene056952.t1	MTR_4g070080	45.161	62	33	1	10	70	9	70	8.22e-12	57.0
MS.gene056952.t1	MTR_4g070190	43.548	62	34	1	10	70	11	72	2.04e-11	55.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene056952.t1	AT5G19960	61.429	70	27	0	1	70	1	70	7.50e-27	99.8
MS.gene056952.t1	AT3G47120	39.394	66	39	1	5	69	34	99	5.78e-11	56.2
MS.gene056952.t1	AT3G47120	39.394	66	39	1	5	69	34	99	7.54e-11	55.8

Find 17 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGCACTGTCTTCAATTTGTA+TGG	0.310277	8.4:+15432114	MS.gene056952:CDS
GGGAAGACCACCAACGTAAA+TGG	0.322442	8.4:-15432067	None:intergenic
ATCAATGACCAACGTACACT+TGG	0.451190	8.4:+15432299	MS.gene056952:CDS
TGTTTCATCAGTGGCGTCGA+AGG	0.487655	8.4:-15432088	None:intergenic
TTGTAACTTTCACAAACCCT+AGG	0.525397	8.4:+15432336	MS.gene056952:CDS
TAGCATCAATTGCAGACCTA+GGG	0.528965	8.4:-15432352	None:intergenic
GATAGCTCCATTTACGTTGG+TGG	0.531498	8.4:+15432060	MS.gene056952:CDS
ATAGCATCAATTGCAGACCT+AGG	0.550720	8.4:-15432353	None:intergenic
GACTACCTTGACATCAAGAA+TGG	0.552219	8.4:-15432139	None:intergenic
GTTTCATCAGTGGCGTCGAA+GGG	0.576277	8.4:-15432087	None:intergenic
TGGCGCCATTCTTGATGTCA+AGG	0.589109	8.4:+15432134	MS.gene056952:CDS
TCAATGACCAACGTACACTT+GGG	0.603480	8.4:+15432300	MS.gene056952:CDS
AGTGCGAATTGTTTCATCAG+TGG	0.616718	8.4:-15432097	None:intergenic
TCAGTTCCACAAAATGACAC+TGG	0.624699	8.4:+15432032	None:intergenic
AGCATTTCCCAAGTGTACGT+TGG	0.632194	8.4:-15432307	None:intergenic
GATGCTATCAACGATATGAA+CGG	0.633466	8.4:+15432368	MS.gene056952:CDS
GATGATAGCTCCATTTACGT+TGG	0.659955	8.4:+15432057	MS.gene056952:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGAAATTAAATAATTAACA+AGG	-	chr8.4:15432202-15432221	None:intergenic	10.0%
!!	AGAAATTAAATAATTAACAA+GGG	-	chr8.4:15432201-15432220	None:intergenic	10.0%
!!	AATAATCAATTCAAGTAATT+GGG	+	chr8.4:15432227-15432246	MS.gene056952:intron	15.0%
!!	TAATAATCAATTCAAGTAAT+TGG	+	chr8.4:15432226-15432245	MS.gene056952:intron	15.0%
	GATGCTATCAACGATATGAA+CGG	+	chr8.4:15432368-15432387	MS.gene056952:CDS	35.0%
	TTGTAACTTTCACAAACCCT+AGG	+	chr8.4:15432336-15432355	MS.gene056952:CDS	35.0%
	AGTGCGAATTGTTTCATCAG+TGG	-	chr8.4:15432100-15432119	None:intergenic	40.0%
	ATAGCATCAATTGCAGACCT+AGG	-	chr8.4:15432356-15432375	None:intergenic	40.0%
	ATCAATGACCAACGTACACT+TGG	+	chr8.4:15432299-15432318	MS.gene056952:CDS	40.0%
	CGCACTGTCTTCAATTTGTA+TGG	+	chr8.4:15432114-15432133	MS.gene056952:CDS	40.0%
	GACTACCTTGACATCAAGAA+TGG	-	chr8.4:15432142-15432161	None:intergenic	40.0%
	GATGATAGCTCCATTTACGT+TGG	+	chr8.4:15432057-15432076	MS.gene056952:CDS	40.0%
	TAGCATCAATTGCAGACCTA+GGG	-	chr8.4:15432355-15432374	None:intergenic	40.0%
	TCAATGACCAACGTACACTT+GGG	+	chr8.4:15432300-15432319	MS.gene056952:CDS	40.0%
	AGCATTTCCCAAGTGTACGT+TGG	-	chr8.4:15432310-15432329	None:intergenic	45.0%
	GATAGCTCCATTTACGTTGG+TGG	+	chr8.4:15432060-15432079	MS.gene056952:CDS	45.0%
	GGGAAGACCACCAACGTAAA+TGG	-	chr8.4:15432070-15432089	None:intergenic	50.0%
	TGGCGCCATTCTTGATGTCA+AGG	+	chr8.4:15432134-15432153	MS.gene056952:CDS	50.0%
!	GTTTCATCAGTGGCGTCGAA+GGG	-	chr8.4:15432090-15432109	None:intergenic	50.0%
!	TGTTTCATCAGTGGCGTCGA+AGG	-	chr8.4:15432091-15432110	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	15432045	15432394	15432045	ID=MS.gene056952
chr8.4	mRNA	15432045	15432394	15432045	ID=MS.gene056952.t1;Parent=MS.gene056952
chr8.4	exon	15432045	15432155	15432045	ID=MS.gene056952.t1.exon1;Parent=MS.gene056952.t1
chr8.4	CDS	15432045	15432155	15432045	ID=cds.MS.gene056952.t1;Parent=MS.gene056952.t1
chr8.4	exon	15432296	15432394	15432296	ID=MS.gene056952.t1.exon2;Parent=MS.gene056952.t1
chr8.4	CDS	15432296	15432394	15432296	ID=cds.MS.gene056952.t1;Parent=MS.gene056952.t1

Gene Sequence	>MS.gene056952 ATGACACTGGACGATGATAGCTCCATTTACGTTGGTGGTCTTCCCTTCGACGCCACTGATGAAACAATTCGCACTGTCTTCAATTTGTATGGCGCCATTCTTGATGTCAAGGTAGTCTTTCACTTTTTTTAAATTTTTAAATTTTTTATTAAACCCTTGTTAATTATTTAATTTCTTTAGCTAATAATCAATTCAAGTAATTGGGTTTTCATTTTTATTGATGAGTTTTTTTTTTTTTTTTTTAATTTCAGATTATCAATGACCAACGTACACTTGGGAAATGCTATTGTTTTGTAACTTTCACAAACCCTAGGTCTGCAATTGATGCTATCAACGATATGAACGGAAGA
Protein sequence	>MS.gene056952.t1 MTLDDDSSIYVGGLPFDATDETIRTVFNLYGAILDVKIINDQRTLGKCYCFVTFTNPRSAIDAINDMNGR