AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene06697


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06697.t1 MTR_4g120840 47.872 94 48 1 1 93 13 106 4.17e-23 89.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 38 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAGAAAGGTTGTGTTTA+GGG 0.236468 3.3:-76423634 MS.gene06697:CDS
TACAATGGTTAGAGATGTTT+TGG 0.255930 3.3:-76423310 MS.gene06697:CDS
CATTGAGGGATTGGGGTCTT+AGG 0.272322 3.3:-76423380 MS.gene06697:CDS
CTTCAAGAAAGGTTGTGTTT+AGG 0.331468 3.3:-76423635 MS.gene06697:CDS
TATCAATGAAGTGGGCAGTT+AGG 0.342841 3.3:+76423490 None:intergenic
TTTAGAGTCATTGAGGGATT+GGG 0.363471 3.3:-76423388 MS.gene06697:CDS
ATCAATGAAGTGGGCAGTTA+GGG 0.365154 3.3:+76423491 None:intergenic
GGCAGAAACCGGAGAAGCTT+TGG 0.383249 3.3:-76424457 MS.gene06697:CDS
AACCATTGTAGCCACATCAT+TGG 0.433510 3.3:+76423323 None:intergenic
CTAAACACAACCTTTCTTGA+AGG 0.439583 3.3:+76423636 None:intergenic
ACAAGTGTCCAAAGCTTCTC+CGG 0.453626 3.3:+76424449 None:intergenic
TTTGAATACCCCTTCAAGAA+AGG 0.454546 3.3:-76423646 MS.gene06697:CDS
ATTGTGTCACCCGTGTGGTT+TGG 0.454713 3.3:+76423423 None:intergenic
TAAAAGATGGAGGCAGAAAC+CGG 0.456738 3.3:-76424468 None:intergenic
TCTAAAGCTACTGATGAAAC+AGG 0.469935 3.3:+76423282 None:intergenic
CTTGAAGGGGTATTCAAATG+AGG 0.476544 3.3:+76423651 None:intergenic
AACCACACGGGTGACACAAT+AGG 0.486470 3.3:-76423420 MS.gene06697:CDS
ATTGGACGAAGCATTGTTAA+CGG 0.496491 3.3:+76423341 None:intergenic
CCTAAAAGCATCACCTTGCA+AGG 0.515004 3.3:+76423764 None:intergenic
TAAACACAACCTTTCTTGAA+GGG 0.523423 3.3:+76423637 None:intergenic
TCCCTTCAGTATCAATGAAG+TGG 0.525870 3.3:+76423481 None:intergenic
TTAGAGTCATTGAGGGATTG+GGG 0.541307 3.3:-76423387 MS.gene06697:CDS
CCCTTCAGTATCAATGAAGT+GGG 0.551659 3.3:+76423482 None:intergenic
GCCCACTTCATTGATACTGA+AGG 0.572131 3.3:-76423483 MS.gene06697:CDS
TAGCATTCACTACCGACCGC+TGG 0.579833 3.3:-76423542 MS.gene06697:CDS
GTCCAATGATGTGGCTACAA+TGG 0.588721 3.3:-76423325 MS.gene06697:CDS
AAACACAACCTTTCTTGAAG+GGG 0.592722 3.3:+76423638 None:intergenic
CCCACTTCATTGATACTGAA+GGG 0.608037 3.3:-76423482 MS.gene06697:CDS
CAATGCTTCGTCCAATGATG+TGG 0.608816 3.3:-76423334 MS.gene06697:CDS
GCAGCTGTTGCACCCTTGCA+AGG 0.611569 3.3:-76423777 MS.gene06697:CDS
TACTATAGAAGTCCAGCGGT+CGG 0.629469 3.3:+76423530 None:intergenic
CTAAAAGCATCACCTTGCAA+GGG 0.630736 3.3:+76423765 None:intergenic
AGCTACTGATGAAACAGGTG+TGG 0.636450 3.3:+76423287 None:intergenic
TAGTTTAGTTCCAAACCACA+CGG 0.642752 3.3:-76423433 MS.gene06697:CDS
AGTTTAGTTCCAAACCACAC+GGG 0.660320 3.3:-76423432 MS.gene06697:CDS
TCAATGAAGTGGGCAGTTAG+GGG 0.667038 3.3:+76423492 None:intergenic
GACCTATTGTGTCACCCGTG+TGG 0.681347 3.3:+76423418 None:intergenic
AGTTTACTATAGAAGTCCAG+CGG 0.728280 3.3:+76423526 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTACTACTATTTTCATAATT+TGG - chr3.3:76423512-76423531 MS.gene06697:CDS 15.0%
!! AATTGTTGAACATATCTTAA+AGG + chr3.3:76423798-76423817 None:intergenic 20.0%
!!! AGTATGTTTTAATATGATTC+TGG - chr3.3:76423867-76423886 MS.gene06697:intron 20.0%
! AATAAAGATGTCATTGTATG+TGG + chr3.3:76423582-76423601 None:intergenic 25.0%
! AGCATATTAAAACTCATTGT+TGG + chr3.3:76423769-76423788 None:intergenic 25.0%
! TACAATGACATCTTTATTCA+TGG - chr3.3:76423584-76423603 MS.gene06697:CDS 25.0%
! TATGAAGTTGTTTGTTTGTA+TGG - chr3.3:76423307-76423326 MS.gene06697:CDS 25.0%
!! AGCAAAAATTTGTTTCCAAA+CGG - chr3.3:76423626-76423645 MS.gene06697:CDS 25.0%
!! TTTTCTCTTAAAATCAGCTA+TGG - chr3.3:76423678-76423697 MS.gene06697:intron 25.0%
!!! AAAAAGTTTTAGAGTCATTG+AGG - chr3.3:76424297-76424316 MS.gene06697:intron 25.0%
!!! AAAAGTTTTAGAGTCATTGA+GGG - chr3.3:76424298-76424317 MS.gene06697:intron 25.0%
AAGCTAGATTCAATCTACAT+AGG + chr3.3:76423436-76423455 None:intergenic 30.0%
AGCACAGGAAATATCAAAAA+AGG - chr3.3:76424443-76424462 MS.gene06697:intron 30.0%
AGTAAATGGAGTTACAACTA+TGG - chr3.3:76423705-76423724 MS.gene06697:intron 30.0%
CTGAAGGGAAATATCAAAAA+AGG - chr3.3:76424225-76424244 MS.gene06697:intron 30.0%
TAAACACAACCTTTCTTGAA+GGG + chr3.3:76424058-76424077 None:intergenic 30.0%
TATTCAAATGAGGCTACAAA+TGG + chr3.3:76424034-76424053 None:intergenic 30.0%
TGTTTGATCAAGTGTTCATT+AGG - chr3.3:76423392-76423411 MS.gene06697:CDS 30.0%
TTCAAGAAAGGTTGTGTTTA+GGG - chr3.3:76424058-76424077 MS.gene06697:intron 30.0%
! CTCTAAAAGGAAGTTTAGAA+AGG + chr3.3:76423977-76423996 None:intergenic 30.0%
! GATTGAATCTAGCTTATGTT+TGG - chr3.3:76423441-76423460 MS.gene06697:CDS 30.0%
! TACAATGGTTAGAGATGTTT+TGG - chr3.3:76424382-76424401 MS.gene06697:intron 30.0%
!!! TGGATCTAGAGAAGTATTTT+GGG + chr3.3:76423562-76423581 None:intergenic 30.0%
AAACACAACCTTTCTTGAAG+GGG + chr3.3:76424057-76424076 None:intergenic 35.0%
AAATGGAGTTACAACTATGG+AGG - chr3.3:76423708-76423727 MS.gene06697:intron 35.0%
AATGGAGTTACAACTATGGA+GGG - chr3.3:76423709-76423728 MS.gene06697:intron 35.0%
AGTTTACTATAGAAGTCCAG+CGG + chr3.3:76424169-76424188 None:intergenic 35.0%
ATAGGACTAAACCACATTGA+AGG + chr3.3:76423418-76423437 None:intergenic 35.0%
CTAAACACAACCTTTCTTGA+AGG + chr3.3:76424059-76424078 None:intergenic 35.0%
CTCTTAAAATCAGCTATGGA+AGG - chr3.3:76423682-76423701 MS.gene06697:intron 35.0%
CTTCAAGAAAGGTTGTGTTT+AGG - chr3.3:76424057-76424076 MS.gene06697:intron 35.0%
TGAGACTCAACTACTCTAAA+AGG + chr3.3:76423990-76424009 None:intergenic 35.0%
TTTGAATACCCCTTCAAGAA+AGG - chr3.3:76424046-76424065 MS.gene06697:intron 35.0%
! AAACAAATTTTTGCTGCTGC+TGG + chr3.3:76423621-76423640 None:intergenic 35.0%
! TAGTTTAGTTCCAAACCACA+CGG - chr3.3:76424259-76424278 MS.gene06697:intron 35.0%
! TCTAAAGCTACTGATGAAAC+AGG + chr3.3:76424413-76424432 None:intergenic 35.0%
! TTGGACACTTGTAAGTTTTG+TGG - chr3.3:76423254-76423273 MS.gene06697:CDS 35.0%
! TTTAGAGTCATTGAGGGATT+GGG - chr3.3:76424304-76424323 MS.gene06697:intron 35.0%
!! ATTGGACGAAGCATTGTTAA+CGG + chr3.3:76424354-76424373 None:intergenic 35.0%
!!! GTGGATCTAGAGAAGTATTT+TGG + chr3.3:76423563-76423582 None:intergenic 35.0%
!!! TTTTAGAGTCATTGAGGGAT+TGG - chr3.3:76424303-76424322 MS.gene06697:intron 35.0%
AACCATTGTAGCCACATCAT+TGG + chr3.3:76424372-76424391 None:intergenic 40.0%
ATCAATGAAGTGGGCAGTTA+GGG + chr3.3:76424204-76424223 None:intergenic 40.0%
ATGGAGTTACAACTATGGAG+GGG - chr3.3:76423710-76423729 MS.gene06697:intron 40.0%
CCCACTTCATTGATACTGAA+GGG - chr3.3:76424210-76424229 MS.gene06697:intron 40.0%
CCCTTCAGTATCAATGAAGT+GGG + chr3.3:76424213-76424232 None:intergenic 40.0%
GTATGGCGTGTTCATATCTA+TGG - chr3.3:76423324-76423343 MS.gene06697:CDS 40.0%
TATCAATGAAGTGGGCAGTT+AGG + chr3.3:76424205-76424224 None:intergenic 40.0%
TCAGCTATGGAAGGAGTAAA+TGG - chr3.3:76423691-76423710 MS.gene06697:intron 40.0%
TCCCTTCAGTATCAATGAAG+TGG + chr3.3:76424214-76424233 None:intergenic 40.0%
TGTTCATTAGGCCTTCAATG+TGG - chr3.3:76423404-76423423 MS.gene06697:CDS 40.0%
TTAGAGTCATTGAGGGATTG+GGG - chr3.3:76424305-76424324 MS.gene06697:intron 40.0%
! AGTTTAGTTCCAAACCACAC+GGG - chr3.3:76424260-76424279 MS.gene06697:intron 40.0%
!! CTAAAAGCATCACCTTGCAA+GGG + chr3.3:76423930-76423949 None:intergenic 40.0%
!! CTTGAAGGGGTATTCAAATG+AGG + chr3.3:76424044-76424063 None:intergenic 40.0%
!!! TTAGAGAGCATTTTCAGCAC+AGG - chr3.3:76424428-76424447 MS.gene06697:intron 40.0%
ACAAGTGTCCAAAGCTTCTC+CGG + chr3.3:76423246-76423265 None:intergenic 45.0%
AGCTACTGATGAAACAGGTG+TGG + chr3.3:76424408-76424427 None:intergenic 45.0%
CAATGCTTCGTCCAATGATG+TGG - chr3.3:76424358-76424377 MS.gene06697:intron 45.0%
GCCCACTTCATTGATACTGA+AGG - chr3.3:76424209-76424228 MS.gene06697:intron 45.0%
GTCCAATGATGTGGCTACAA+TGG - chr3.3:76424367-76424386 MS.gene06697:intron 45.0%
TACTATAGAAGTCCAGCGGT+CGG + chr3.3:76424165-76424184 None:intergenic 45.0%
TCAATGAAGTGGGCAGTTAG+GGG + chr3.3:76424203-76424222 None:intergenic 45.0%
TCACACAGATAGTCACCGTT+TGG + chr3.3:76423644-76423663 None:intergenic 45.0%
! CCTTGCAAGGTGATGCTTTT+AGG - chr3.3:76423928-76423947 MS.gene06697:intron 45.0%
!! CCTAAAAGCATCACCTTGCA+AGG + chr3.3:76423931-76423950 None:intergenic 45.0%
!! GCAAGGTGATGCTTTTAGGA+AGG - chr3.3:76423932-76423951 MS.gene06697:intron 45.0%
AACCACACGGGTGACACAAT+AGG - chr3.3:76424272-76424291 MS.gene06697:intron 50.0%
ATTGTGTCACCCGTGTGGTT+TGG + chr3.3:76424272-76424291 None:intergenic 50.0%
CATTGAGGGATTGGGGTCTT+AGG - chr3.3:76424312-76424331 MS.gene06697:intron 50.0%
! ACTCAAGTTGCAGCAGCTAC+TGG - chr3.3:76423735-76423754 MS.gene06697:intron 50.0%
GACCTATTGTGTCACCCGTG+TGG + chr3.3:76424277-76424296 None:intergenic 55.0%
GGCAGAAACCGGAGAAGCTT+TGG - chr3.3:76423235-76423254 MS.gene06697:CDS 55.0%
TAGCATTCACTACCGACCGC+TGG - chr3.3:76424150-76424169 MS.gene06697:intron 55.0%
GCAGCTGTTGCACCCTTGCA+AGG - chr3.3:76423915-76423934 MS.gene06697:intron 60.0%
GTTGCAGCAGCTACTGGAGC+AGG - chr3.3:76423741-76423760 MS.gene06697:intron 60.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 76423230 76424484 76423230 ID=MS.gene06697
chr3.3 mRNA 76423230 76424484 76423230 ID=MS.gene06697.t1;Parent=MS.gene06697
chr3.3 exon 76424451 76424484 76424451 ID=MS.gene06697.t1.exon1;Parent=MS.gene06697.t1
chr3.3 CDS 76424451 76424484 76424451 ID=cds.MS.gene06697.t1;Parent=MS.gene06697.t1
chr3.3 exon 76423746 76423819 76423746 ID=MS.gene06697.t1.exon2;Parent=MS.gene06697.t1
chr3.3 CDS 76423746 76423819 76423746 ID=cds.MS.gene06697.t1;Parent=MS.gene06697.t1
chr3.3 exon 76423230 76423673 76423230 ID=MS.gene06697.t1.exon3;Parent=MS.gene06697.t1
chr3.3 CDS 76423230 76423673 76423230 ID=cds.MS.gene06697.t1;Parent=MS.gene06697.t1
Gene Sequence

>MS.gene06697

ATGGAGGCAGAAACCGGAGAAGCTTTGGACACTTGTAAGTTTTGTGGTGATTTGTATTTTTTTTTTTTATGAAAATCTATGAAGTTGTTTGTTTGTATGGCGTGTTCATATCTATGGAATTTAAGAAATTATGAGTCATGTGTATAATTATTTGTGTGTACGTGTTTGATCAAGTGTTCATTAGGCCTTCAATGTGGTTTAGTCCTATGTAGATTGAATCTAGCTTATGTTTGGTATGTTTATTTGCATTCTGTTTACTACTATTAAAATTCTACAATATGTTTACTACTATTTTCATAATTTGGCAGTTGATAACACTCAATTTGCTACCCAAAATACTTCTCTAGATCCACATACAATGACATCTTTATTCATGGCAGTAGCTATTCCAGCAGCAGCAAAAATTTGTTTCCAAACGGTGACTATCTGTGTGATTTAACTGTTTTTTTTTTCTCTTAAAATCAGCTATGGAAGGAGTAAATGGAGTTACAACTATGGAGGGGTAACTCAAGTTGCAGCAGCTACTGGAGCAGGAACCAACAATGAGTTTTAATATGCTTATCTGCCTTTAAGATATGTTCAACAATTAACATGTGTTATTAAGTTCTGTTATGTGTAAATTTTTTATTCACATAACAGTATGTTTTAATATGATTCTGGTGCAGCTGCAATTGTTGAAACTGAAGCAGCTGTTGCACCCTTGCAAGGTGATGCTTTTAGGAAGGCAATTGCTAGATTTGTTATCCTTTCTAAACTTCCTTTTAGAGTAGTTGAGTCTCATAGCTTCAAGCGCTTATGTAACCATTTGTAGCCTCATTTGAATACCCCTTCAAGAAAGGTTGTGTTTAGGGATTGTTTTAAGATGTATCAAGATGAAAAAGTGAAGCTCAAAGCTTATTTTAAATCTGATTGTAGTAAGCTAGCATTCACTACCGACCGCTGGACTTCTATAGTAAACTTTAGCTACATTCCCCTAACTGCCCACTTCATTGATACTGAAGGGAAATATCAAAAAAGGATTATTAGCTTTAGTTTAGTTCCAAACCACACGGGTGACACAATAGGTCAAAAAGTTTTAGAGTCATTGAGGGATTGGGGTCTTAGGAATGTGTCTACAGTCACCGTTAACAATGCTTCGTCCAATGATGTGGCTACAATGGTTAGAGATGTTTTGGCCACACCTGTTTCATCAGTAGCTTTAGAGAGCATTTTCAGCACAGGAAATATCAAAAAAGGATTATTAGCTTTAGTTTAG

Protein sequence

>MS.gene06697.t1

MEAETGEALDTSAIVETEAAVAPLQGDAFRKAIARFPHLNTPSRKVVFRDCFKMYQDEKVKLKAYFKSDCSKLAFTTDRWTSIVNFSYIPLTAHFIDTEGKYQKRIISFSLVPNHTGDTIGQKVLESLRDWGLRNVSTVTVNNASSNDVATMVRDVLATPVSSVALESIFSTGNIKKGLLALV