Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99887.t1 | XP_003601051.1 | 84.4 | 435 | 64 | 1 | 1 | 431 | 1 | 435 | 1.80E-131 | 479.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99887.t1 | O23461 | 54.9 | 71 | 32 | 0 | 450 | 520 | 847 | 917 | 5.2e-15 | 84.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99887.t1 | G7J309 | 84.4 | 435 | 64 | 1 | 1 | 431 | 1 | 435 | 1.3e-131 | 479.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene99887 | 0.837408 | 5.29E-57 | -1.69E-46 |
| MS.gene049159 | MS.gene99887 | 0.853405 | 2.44E-61 | -1.69E-46 |
| MS.gene049341 | MS.gene99887 | 0.82406 | 9.90E-54 | -1.69E-46 |
| MS.gene049382 | MS.gene99887 | 0.805385 | 1.37E-49 | -1.69E-46 |
| MS.gene049435 | MS.gene99887 | 0.821973 | 3.04E-53 | -1.69E-46 |
| MS.gene049501 | MS.gene99887 | 0.818223 | 2.19E-52 | -1.69E-46 |
| MS.gene049850 | MS.gene99887 | 0.859542 | 3.85E-63 | -1.69E-46 |
| MS.gene049887 | MS.gene99887 | 0.801033 | 1.09E-48 | -1.69E-46 |
| MS.gene049979 | MS.gene99887 | 0.808044 | 3.76E-50 | -1.69E-46 |
| MS.gene050020 | MS.gene99887 | 0.835036 | 2.12E-56 | -1.69E-46 |
| MS.gene050226 | MS.gene99887 | 0.811061 | 8.45E-51 | -1.69E-46 |
| MS.gene050228 | MS.gene99887 | 0.80404 | 2.61E-49 | -1.69E-46 |
| MS.gene050442 | MS.gene99887 | 0.817604 | 3.03E-52 | -1.69E-46 |
| MS.gene050586 | MS.gene99887 | 0.821267 | 4.42E-53 | -1.69E-46 |
| MS.gene050689 | MS.gene99887 | 0.852413 | 4.69E-61 | -1.69E-46 |
| MS.gene050719 | MS.gene99887 | 0.807926 | 3.98E-50 | -1.69E-46 |
| MS.gene050721 | MS.gene99887 | 0.808619 | 2.83E-50 | -1.69E-46 |
| MS.gene050724 | MS.gene99887 | 0.810289 | 1.24E-50 | -1.69E-46 |
| MS.gene050762 | MS.gene99887 | 0.808263 | 3.38E-50 | -1.69E-46 |
| MS.gene050763 | MS.gene99887 | 0.807368 | 5.23E-50 | -1.69E-46 |
| MS.gene050780 | MS.gene99887 | 0.802752 | 4.83E-49 | -1.69E-46 |
| MS.gene050807 | MS.gene99887 | 0.861728 | 8.36E-64 | -1.69E-46 |
| MS.gene050808 | MS.gene99887 | 0.810797 | 9.64E-51 | -1.69E-46 |
| MS.gene050815 | MS.gene99887 | 0.828119 | 1.07E-54 | -1.69E-46 |
| MS.gene050959 | MS.gene99887 | 0.806595 | 7.62E-50 | -1.69E-46 |
| MS.gene051102 | MS.gene99887 | 0.840024 | 1.12E-57 | -1.69E-46 |
| MS.gene051180 | MS.gene99887 | 0.852052 | 5.95E-61 | -1.69E-46 |
| MS.gene051198 | MS.gene99887 | 0.815566 | 8.66E-52 | -1.69E-46 |
| MS.gene051210 | MS.gene99887 | 0.80243 | 5.63E-49 | -1.69E-46 |
| MS.gene051243 | MS.gene99887 | 0.804855 | 1.77E-49 | -1.69E-46 |
| MS.gene051247 | MS.gene99887 | 0.809262 | 2.06E-50 | -1.69E-46 |
| MS.gene051328 | MS.gene99887 | 0.83003 | 3.69E-55 | -1.69E-46 |
| MS.gene051388 | MS.gene99887 | 0.81257 | 3.97E-51 | -1.69E-46 |
| MS.gene051577 | MS.gene99887 | 0.812402 | 4.32E-51 | -1.69E-46 |
| MS.gene05157 | MS.gene99887 | 0.833221 | 6.04E-56 | -1.69E-46 |
| MS.gene051719 | MS.gene99887 | 0.811424 | 7.05E-51 | -1.69E-46 |
| MS.gene051720 | MS.gene99887 | 0.828994 | 6.60E-55 | -1.69E-46 |
| MS.gene051744 | MS.gene99887 | 0.833092 | 6.51E-56 | -1.69E-46 |
| MS.gene051800 | MS.gene99887 | 0.822581 | 2.19E-53 | -1.69E-46 |
| MS.gene052012 | MS.gene99887 | 0.801343 | 9.42E-49 | -1.69E-46 |
| MS.gene052020 | MS.gene99887 | 0.844503 | 7.26E-59 | -1.69E-46 |
| MS.gene052133 | MS.gene99887 | 0.80778 | 4.28E-50 | -1.69E-46 |
| MS.gene052323 | MS.gene99887 | 0.8553 | 6.92E-62 | -1.69E-46 |
| MS.gene052397 | MS.gene99887 | 0.854575 | 1.12E-61 | -1.69E-46 |
| MS.gene052516 | MS.gene99887 | 0.860129 | 2.56E-63 | -1.69E-46 |
| MS.gene052609 | MS.gene99887 | 0.816191 | 6.28E-52 | -1.69E-46 |
| MS.gene052611 | MS.gene99887 | 0.870053 | 1.95E-66 | -1.69E-46 |
| MS.gene052612 | MS.gene99887 | 0.838618 | 2.58E-57 | -1.69E-46 |
| MS.gene052665 | MS.gene99887 | 0.855841 | 4.81E-62 | -1.69E-46 |
| MS.gene052773 | MS.gene99887 | 0.815379 | 9.53E-52 | -1.69E-46 |
| MS.gene052858 | MS.gene99887 | 0.810437 | 1.15E-50 | -1.69E-46 |
| MS.gene052860 | MS.gene99887 | 0.822554 | 2.23E-53 | -1.69E-46 |
| MS.gene052863 | MS.gene99887 | 0.84601 | 2.84E-59 | -1.69E-46 |
| MS.gene052896 | MS.gene99887 | 0.849517 | 3.07E-60 | -1.69E-46 |
| MS.gene053008 | MS.gene99887 | 0.831351 | 1.75E-55 | -1.69E-46 |
| MS.gene053074 | MS.gene99887 | 0.818828 | 1.60E-52 | -1.69E-46 |
| MS.gene053079 | MS.gene99887 | 0.814125 | 1.81E-51 | -1.69E-46 |
| MS.gene053144 | MS.gene99887 | 0.817863 | 2.64E-52 | -1.69E-46 |
| MS.gene053226 | MS.gene99887 | 0.840701 | 7.42E-58 | -1.69E-46 |
| MS.gene053565 | MS.gene99887 | 0.831801 | 1.36E-55 | -1.69E-46 |
| MS.gene053581 | MS.gene99887 | 0.809688 | 1.67E-50 | -1.69E-46 |
| MS.gene05364 | MS.gene99887 | 0.817727 | 2.84E-52 | -1.69E-46 |
| MS.gene054379 | MS.gene99887 | 0.810376 | 1.19E-50 | -1.69E-46 |
| MS.gene054467 | MS.gene99887 | 0.819295 | 1.25E-52 | -1.69E-46 |
| MS.gene054472 | MS.gene99887 | 0.824988 | 5.99E-54 | -1.69E-46 |
| MS.gene054584 | MS.gene99887 | 0.815144 | 1.07E-51 | -1.69E-46 |
| MS.gene05462 | MS.gene99887 | 0.83884 | 2.26E-57 | -1.69E-46 |
| MS.gene054651 | MS.gene99887 | 0.811994 | 5.30E-51 | -1.69E-46 |
| MS.gene05468 | MS.gene99887 | 0.802792 | 4.74E-49 | -1.69E-46 |
| MS.gene054994 | MS.gene99887 | 0.803511 | 3.37E-49 | -1.69E-46 |
| MS.gene05510 | MS.gene99887 | 0.805238 | 1.47E-49 | -1.69E-46 |
| MS.gene055227 | MS.gene99887 | 0.813942 | 1.98E-51 | -1.69E-46 |
| MS.gene055363 | MS.gene99887 | 0.847319 | 1.25E-59 | -1.69E-46 |
| MS.gene055465 | MS.gene99887 | 0.831271 | 1.83E-55 | -1.69E-46 |
| MS.gene055466 | MS.gene99887 | 0.842253 | 2.89E-58 | -1.69E-46 |
| MS.gene055468 | MS.gene99887 | 0.81818 | 2.24E-52 | -1.69E-46 |
| MS.gene055469 | MS.gene99887 | 0.817858 | 2.65E-52 | -1.69E-46 |
| MS.gene055512 | MS.gene99887 | 0.865155 | 7.25E-65 | -1.69E-46 |
| MS.gene055580 | MS.gene99887 | -0.807798 | 4.24E-50 | -1.69E-46 |
| MS.gene055623 | MS.gene99887 | 0.829333 | 5.46E-55 | -1.69E-46 |
| MS.gene055818 | MS.gene99887 | 0.834153 | 3.53E-56 | -1.69E-46 |
| MS.gene055926 | MS.gene99887 | 0.814508 | 1.49E-51 | -1.69E-46 |
| MS.gene056043 | MS.gene99887 | -0.801035 | 1.09E-48 | -1.69E-46 |
| MS.gene05604 | MS.gene99887 | 0.802589 | 5.22E-49 | -1.69E-46 |
| MS.gene056173 | MS.gene99887 | 0.819146 | 1.35E-52 | -1.69E-46 |
| MS.gene056308 | MS.gene99887 | 0.815981 | 7.00E-52 | -1.69E-46 |
| MS.gene056366 | MS.gene99887 | 0.812773 | 3.58E-51 | -1.69E-46 |
| MS.gene056507 | MS.gene99887 | 0.832035 | 1.19E-55 | -1.69E-46 |
| MS.gene056543 | MS.gene99887 | 0.80381 | 2.92E-49 | -1.69E-46 |
| MS.gene056553 | MS.gene99887 | 0.850279 | 1.88E-60 | -1.69E-46 |
| MS.gene056589 | MS.gene99887 | 0.805062 | 1.60E-49 | -1.69E-46 |
| MS.gene056643 | MS.gene99887 | 0.819712 | 1.01E-52 | -1.69E-46 |
| MS.gene056760 | MS.gene99887 | 0.849155 | 3.88E-60 | -1.69E-46 |
| MS.gene056952 | MS.gene99887 | 0.802593 | 5.21E-49 | -1.69E-46 |
| MS.gene057057 | MS.gene99887 | 0.800464 | 1.42E-48 | -1.69E-46 |
| MS.gene057140 | MS.gene99887 | 0.813164 | 2.94E-51 | -1.69E-46 |
| MS.gene057444 | MS.gene99887 | 0.821922 | 3.12E-53 | -1.69E-46 |
| MS.gene057446 | MS.gene99887 | 0.842289 | 2.83E-58 | -1.69E-46 |
| MS.gene057869 | MS.gene99887 | 0.863193 | 2.97E-64 | -1.69E-46 |
| MS.gene057915 | MS.gene99887 | 0.852061 | 5.91E-61 | -1.69E-46 |
| MS.gene058010 | MS.gene99887 | 0.838224 | 3.27E-57 | -1.69E-46 |
| MS.gene058168 | MS.gene99887 | 0.809871 | 1.53E-50 | -1.69E-46 |
| MS.gene05847 | MS.gene99887 | 0.815535 | 8.80E-52 | -1.69E-46 |
| MS.gene058625 | MS.gene99887 | 0.820298 | 7.39E-53 | -1.69E-46 |
| MS.gene058636 | MS.gene99887 | 0.807906 | 4.02E-50 | -1.69E-46 |
| MS.gene058829 | MS.gene99887 | 0.801881 | 7.30E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene99887 | 0.821756 | 3.41E-53 | -1.69E-46 |
| MS.gene058982 | MS.gene99887 | 0.818204 | 2.21E-52 | -1.69E-46 |
| MS.gene059006 | MS.gene99887 | 0.802516 | 5.41E-49 | -1.69E-46 |
| MS.gene059128 | MS.gene99887 | 0.802639 | 5.10E-49 | -1.69E-46 |
| MS.gene059331 | MS.gene99887 | 0.808786 | 2.61E-50 | -1.69E-46 |
| MS.gene059335 | MS.gene99887 | 0.806854 | 6.72E-50 | -1.69E-46 |
| MS.gene059713 | MS.gene99887 | 0.830834 | 2.35E-55 | -1.69E-46 |
| MS.gene059725 | MS.gene99887 | 0.823056 | 1.70E-53 | -1.69E-46 |
| MS.gene05978 | MS.gene99887 | 0.849002 | 4.28E-60 | -1.69E-46 |
| MS.gene059842 | MS.gene99887 | 0.835939 | 1.25E-56 | -1.69E-46 |
| MS.gene059937 | MS.gene99887 | 0.862869 | 3.73E-64 | -1.69E-46 |
| MS.gene060310 | MS.gene99887 | 0.806969 | 6.35E-50 | -1.69E-46 |
| MS.gene060312 | MS.gene99887 | 0.80291 | 4.48E-49 | -1.69E-46 |
| MS.gene060322 | MS.gene99887 | 0.808843 | 2.54E-50 | -1.69E-46 |
| MS.gene06052 | MS.gene99887 | 0.813113 | 3.02E-51 | -1.69E-46 |
| MS.gene060587 | MS.gene99887 | 0.834208 | 3.42E-56 | -1.69E-46 |
| MS.gene060637 | MS.gene99887 | 0.828539 | 8.50E-55 | -1.69E-46 |
| MS.gene060683 | MS.gene99887 | 0.813593 | 2.37E-51 | -1.69E-46 |
| MS.gene060685 | MS.gene99887 | 0.810739 | 9.93E-51 | -1.69E-46 |
| MS.gene060803 | MS.gene99887 | 0.847865 | 8.82E-60 | -1.69E-46 |
| MS.gene060998 | MS.gene99887 | 0.818254 | 2.16E-52 | -1.69E-46 |
| MS.gene061006 | MS.gene99887 | 0.803243 | 3.83E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene007964 | MS.gene99887 | PPI |
| MS.gene039956 | MS.gene99887 | PPI |
| MS.gene031884 | MS.gene99887 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99887.t1 | MTR_3g072480 | 94.260 | 453 | 22 | 1 | 1 | 449 | 1 | 453 | 0.0 | 821 |
| MS.gene99887.t1 | MTR_1g009190 | 74.648 | 71 | 18 | 0 | 450 | 520 | 781 | 851 | 4.50e-26 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99887.t1 | AT1G44780 | 46.667 | 435 | 189 | 9 | 10 | 439 | 17 | 413 | 8.68e-101 | 315 |
| MS.gene99887.t1 | AT1G44780 | 46.437 | 435 | 191 | 8 | 10 | 439 | 17 | 414 | 1.39e-100 | 314 |
| MS.gene99887.t1 | AT4G08310 | 47.073 | 427 | 203 | 9 | 16 | 437 | 34 | 442 | 5.45e-95 | 301 |
| MS.gene99887.t1 | AT4G16130 | 54.930 | 71 | 32 | 0 | 450 | 520 | 847 | 917 | 2.83e-16 | 83.6 |
| MS.gene99887.t1 | AT3G42850 | 58.065 | 62 | 26 | 0 | 459 | 520 | 780 | 841 | 3.76e-14 | 76.6 |
| MS.gene99887.t1 | AT3G42850 | 58.065 | 62 | 26 | 0 | 459 | 520 | 789 | 850 | 3.87e-14 | 76.6 |
Find 132 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATTGCTCGAGAAAGATTT+AGG | 0.188651 | 3.2:+65290496 | MS.gene99887:CDS |
| TTTACTTATAAACTTCTTAA+AGG | 0.192372 | 3.2:-65292293 | None:intergenic |
| CCCATTGCCTTCTGCTATCT+TGG | 0.207283 | 3.2:-65291961 | None:intergenic |
| TCTTCTGATGCTGCTTTATC+AGG | 0.212935 | 3.2:-65292676 | None:intergenic |
| TGACCACCATTAATGGTTTC+AGG | 0.229197 | 3.2:-65298947 | None:intergenic |
| ATATTTATATACTTCACTTT+TGG | 0.230192 | 3.2:-65300628 | None:intergenic |
| CCCTGAAGATGAGGAGAAAA+TGG | 0.258537 | 3.2:+65291884 | MS.gene99887:CDS |
| TTTAATAGCTTTCTTAATTA+GGG | 0.269138 | 3.2:-65292006 | None:intergenic |
| TCTGAGGAGGGCGGGGAAAA+AGG | 0.296429 | 3.2:+65291798 | MS.gene99887:CDS |
| TAGAAATCAAAGAAGTAAAT+AGG | 0.302925 | 3.2:+65294018 | MS.gene99887:intron |
| TCCACTGGAATCTTGGGCTT+TGG | 0.303298 | 3.2:-65294140 | None:intergenic |
| GGGTAGTCTTCGCCGATTAT+TGG | 0.304072 | 3.2:+65292234 | MS.gene99887:CDS |
| ACTAGTTTCCACTGGAATCT+TGG | 0.310141 | 3.2:-65294147 | None:intergenic |
| CTAGTTTCCACTGGAATCTT+GGG | 0.332663 | 3.2:-65294146 | None:intergenic |
| GTTTCAAAACAGGTAATCTT+AGG | 0.341465 | 3.2:+65299321 | MS.gene99887:intron |
| GACAGCCTTAACTTATTTGT+TGG | 0.341791 | 3.2:-65299410 | None:intergenic |
| GGAGTCTGCATCTTGCCTTT+TGG | 0.346161 | 3.2:-65292592 | None:intergenic |
| ACACTTCCTGAAACCATTAA+TGG | 0.347608 | 3.2:+65298941 | MS.gene99887:CDS |
| CGGACAACTAATTAAAGAAT+TGG | 0.355478 | 3.2:+65293211 | MS.gene99887:CDS |
| ATGGAAGATTCTCCGGTATT+AGG | 0.360072 | 3.2:+65291903 | MS.gene99887:CDS |
| TTAGCACTTTCTGCAGGTTC+CGG | 0.360550 | 3.2:-65292445 | None:intergenic |
| CAGTATCGACCGGTTTCTTA+AGG | 0.363832 | 3.2:+65299387 | MS.gene99887:CDS |
| TTACTTATAAACTTCTTAAA+GGG | 0.364968 | 3.2:-65292292 | None:intergenic |
| GCAGAGTTCCTTGGCATACT+AGG | 0.366428 | 3.2:+65299737 | MS.gene99887:CDS |
| TTGTGTCAAGCACACGTAGA+AGG | 0.366741 | 3.2:+65294087 | MS.gene99887:CDS |
| ATTAATGTCTTCTGAAGTTC+CGG | 0.373428 | 3.2:+65292426 | MS.gene99887:CDS |
| TAAGATCTTCCTCCAATAAT+CGG | 0.374296 | 3.2:-65292246 | None:intergenic |
| TTCTTAATTAGGGTCTCATT+TGG | 0.378832 | 3.2:-65291996 | None:intergenic |
| GGAATCTTGGGCTTTGGTGG+TGG | 0.379600 | 3.2:-65294134 | None:intergenic |
| GCTGTGATATGTTCCTGTTT+TGG | 0.381167 | 3.2:-65300560 | None:intergenic |
| AAACTTCAGAAGTGCTTTGA+AGG | 0.385836 | 3.2:-65299189 | None:intergenic |
| CAATCAACCCAGCAAGAAAA+AGG | 0.386513 | 3.2:+65292648 | MS.gene99887:CDS |
| ATCTTGGGCTTTGGTGGTGG+AGG | 0.389017 | 3.2:-65294131 | None:intergenic |
| GATTGCTCGAGAAAGATTTA+GGG | 0.400346 | 3.2:+65290497 | MS.gene99887:CDS |
| ACTGGAATCTTGGGCTTTGG+TGG | 0.401139 | 3.2:-65294137 | None:intergenic |
| TGATACTAAAGCAACTAAAA+AGG | 0.402536 | 3.2:+65292489 | MS.gene99887:CDS |
| TGTCTTCTGTTTGGTGTTGG+TGG | 0.407420 | 3.2:-65300597 | None:intergenic |
| AATGAGGATGCAGCCAAAAC+AGG | 0.407952 | 3.2:+65300547 | MS.gene99887:CDS |
| TGTTTGGTGTTGGTGGCTGC+TGG | 0.409284 | 3.2:-65300590 | None:intergenic |
| AGGTCTGCTTAAAGAACAAA+AGG | 0.410447 | 3.2:+65291923 | MS.gene99887:CDS |
| GCCCTCCTCAGACGTCTTTG+TGG | 0.419370 | 3.2:-65291787 | None:intergenic |
| TAAGGCCAACAAATAAGTTA+AGG | 0.422891 | 3.2:+65299405 | MS.gene99887:CDS |
| TGATTTCAGAAATGTAACTT+TGG | 0.426248 | 3.2:+65292213 | MS.gene99887:intron |
| GATTTCAGAAATGTAACTTT+GGG | 0.430263 | 3.2:+65292214 | MS.gene99887:intron |
| ATTTGTTGGCCTTAAGAAAC+CGG | 0.432578 | 3.2:-65299396 | None:intergenic |
| TTATTTCTGCCACAGTGCTT+AGG | 0.445202 | 3.2:+65291741 | MS.gene99887:intron |
| TACTGCTAACAAAGAATTGA+AGG | 0.450590 | 3.2:-65299369 | None:intergenic |
| CAAAGACGTCTGAGGAGGGC+GGG | 0.462351 | 3.2:+65291790 | MS.gene99887:CDS |
| TTAATCTTTGCAGAGTTCCT+TGG | 0.465492 | 3.2:+65299728 | MS.gene99887:CDS |
| AGCAGGTCCCTGAAAACAAA+CGG | 0.472188 | 3.2:+65293186 | MS.gene99887:CDS |
| AACCTTTCTGATCCTAGAGT+TGG | 0.472353 | 3.2:-65299344 | None:intergenic |
| CGTTTACTGTACACAGGAGT+TGG | 0.472645 | 3.2:-65293013 | None:intergenic |
| CTCTGCAAAGATTAACGAAA+AGG | 0.475429 | 3.2:-65299720 | None:intergenic |
| CCAAGATAGCAGAAGGCAAT+GGG | 0.479554 | 3.2:+65291961 | MS.gene99887:CDS |
| ACATCAATCTAAAGATGAAA+AGG | 0.481334 | 3.2:+65291857 | MS.gene99887:CDS |
| TAGGAAGAAGGCACGGGCCA+AGG | 0.492533 | 3.2:+65294037 | MS.gene99887:CDS |
| TCATATGCGATGCATCCAAC+AGG | 0.493182 | 3.2:-65300512 | None:intergenic |
| CCACAGTGCTTAGGAGAGGC+TGG | 0.495291 | 3.2:+65291750 | MS.gene99887:intron |
| AGTATTCTCTGCTGACGAAT+GGG | 0.496613 | 3.2:-65292746 | None:intergenic |
| TCTTTGCCACTAGTTTCCAC+TGG | 0.500056 | 3.2:-65294155 | None:intergenic |
| TTCATTGATAGGATGTAATG+TGG | 0.504881 | 3.2:-65299047 | None:intergenic |
| CTTCATACCATCTTCTGCCA+TGG | 0.507544 | 3.2:-65290269 | None:intergenic |
| AAATCTGTTATTAAAGCATG+TGG | 0.508183 | 3.2:+65293049 | MS.gene99887:CDS |
| ACAATTCTTAAACTTGCAGC+AGG | 0.510603 | 3.2:-65299225 | None:intergenic |
| GGCAAGATGCAGACTCCTGT+TGG | 0.510887 | 3.2:+65292598 | MS.gene99887:CDS |
| AAAGACGTCTGAGGAGGGCG+GGG | 0.515404 | 3.2:+65291791 | MS.gene99887:CDS |
| ATGATCTACAGACCATGCTT+CGG | 0.516734 | 3.2:+65300487 | MS.gene99887:intron |
| CAAAGAAGTAAATAGGAAGA+AGG | 0.517613 | 3.2:+65294025 | MS.gene99887:CDS |
| GGAGAAAATGGAAGATTCTC+CGG | 0.519838 | 3.2:+65291896 | MS.gene99887:CDS |
| ATCAATACCCTCGAGTTCCT+TGG | 0.520290 | 3.2:-65294054 | None:intergenic |
| TAAGTTAAGGCTGTCGGTGA+TGG | 0.525569 | 3.2:+65299418 | MS.gene99887:CDS |
| GTGAGAAACGCGAGAGCGCA+TGG | 0.525813 | 3.2:-65290338 | None:intergenic |
| GTCTTCAAATCCATCTGAGA+AGG | 0.532832 | 3.2:+65293259 | MS.gene99887:CDS |
| ACCAAGATAGCAGAAGGCAA+TGG | 0.540066 | 3.2:+65291960 | MS.gene99887:CDS |
| TGTGTCAAGCACACGTAGAA+GGG | 0.540469 | 3.2:+65294088 | MS.gene99887:CDS |
| GGTCTGCTTAAAGAACAAAA+GGG | 0.541386 | 3.2:+65291924 | MS.gene99887:CDS |
| TAGTATTCTCTGCTGACGAA+TGG | 0.542456 | 3.2:-65292747 | None:intergenic |
| GTTGGATGCATCGCATATGA+TGG | 0.543104 | 3.2:+65300515 | MS.gene99887:CDS |
| CAATGAAAATTTCCGAGTCA+AGG | 0.543336 | 3.2:+65299063 | MS.gene99887:CDS |
| GCAGGTCCCTGAAAACAAAC+GGG | 0.543917 | 3.2:+65293187 | MS.gene99887:CDS |
| AACTCTAGGATCAGAAAGGT+TGG | 0.546209 | 3.2:+65299346 | MS.gene99887:CDS |
| TAGTCTTCGCCGATTATTGG+AGG | 0.546979 | 3.2:+65292237 | MS.gene99887:CDS |
| GGTAATCTTAGGCCAACTCT+AGG | 0.548290 | 3.2:+65299332 | MS.gene99887:intron |
| TTCTCATCAATAATAGTGAC+AGG | 0.551294 | 3.2:-65299001 | None:intergenic |
| GGCACGGGCCAAGGAACTCG+AGG | 0.554049 | 3.2:+65294046 | MS.gene99887:CDS |
| AACAAATAAGTTAAGGCTGT+CGG | 0.554482 | 3.2:+65299412 | MS.gene99887:CDS |
| TCTTTAAGCAGACCTAATAC+CGG | 0.555361 | 3.2:-65291915 | None:intergenic |
| AGAGAGACCATGGCAGAAGA+TGG | 0.563600 | 3.2:+65290262 | None:intergenic |
| GACCATGCTTCGGATCCTGT+TGG | 0.564365 | 3.2:+65300497 | MS.gene99887:intron |
| TTCAGATGAAGAGAGTGAAG+AGG | 0.569278 | 3.2:+65292537 | MS.gene99887:CDS |
| ACAAGAGACTGAAGGACAGA+AGG | 0.569598 | 3.2:+65291830 | MS.gene99887:CDS |
| AGTAAATAGGAAGAAGGCAC+GGG | 0.570160 | 3.2:+65294031 | MS.gene99887:CDS |
| ATGAGAAGTGTACTTACGTA+GGG | 0.570522 | 3.2:+65299017 | MS.gene99887:CDS |
| AAGTAAATAGGAAGAAGGCA+CGG | 0.576297 | 3.2:+65294030 | MS.gene99887:CDS |
| ATGATGGAACTAAAGAAATG+AGG | 0.579539 | 3.2:+65300531 | MS.gene99887:CDS |
| CAAGAAACCAAGATAGCAGA+AGG | 0.585200 | 3.2:+65291954 | MS.gene99887:CDS |
| TCTTCAAATCCATCTGAGAA+GGG | 0.588082 | 3.2:+65293260 | MS.gene99887:CDS |
| AAAGGATCTCCCTGAAGATG+AGG | 0.591029 | 3.2:+65291875 | MS.gene99887:CDS |
| AGAGACTGAAGGACAGAAGG+AGG | 0.595213 | 3.2:+65291833 | MS.gene99887:CDS |
| GAAGAAATACTATCTAGAGA+AGG | 0.598424 | 3.2:+65293233 | MS.gene99887:CDS |
| ATCCAACAGGATCCGAAGCA+TGG | 0.600520 | 3.2:-65300499 | None:intergenic |
| GCCACAAAGACGTCTGAGGA+GGG | 0.603177 | 3.2:+65291786 | MS.gene99887:CDS |
| TCATTGATAGGATGTAATGT+GGG | 0.603772 | 3.2:-65299046 | None:intergenic |
| ACAAAGACGTCTGAGGAGGG+CGG | 0.604069 | 3.2:+65291789 | MS.gene99887:CDS |
| GGCCAACTCTAGGATCAGAA+AGG | 0.608310 | 3.2:+65299342 | MS.gene99887:CDS |
| GTCTGCTTAAAGAACAAAAG+GGG | 0.608849 | 3.2:+65291925 | MS.gene99887:CDS |
| TCTTTGTTAGCAGTATCGAC+CGG | 0.610583 | 3.2:+65299377 | MS.gene99887:CDS |
| TATAAGTAAAGAGCTTGATG+AGG | 0.612914 | 3.2:+65292306 | MS.gene99887:CDS |
| ATAAAGTCCTAACCTTGACT+CGG | 0.617132 | 3.2:-65299075 | None:intergenic |
| TGCCACAAAGACGTCTGAGG+AGG | 0.617500 | 3.2:+65291785 | MS.gene99887:CDS |
| TTATTAAAGCATGTGGGATG+AGG | 0.618988 | 3.2:+65293056 | MS.gene99887:CDS |
| GTCCCTGAAAACAAACGGGA+CGG | 0.623725 | 3.2:+65293191 | MS.gene99887:CDS |
| ATAAGGAACCTAGTATGCCA+AGG | 0.627054 | 3.2:-65299745 | None:intergenic |
| CATACTCTGCATTAGCCTTG+AGG | 0.627628 | 3.2:-65292043 | None:intergenic |
| TATCAAACAATCTTTAGACA+AGG | 0.630540 | 3.2:+65290555 | MS.gene99887:CDS |
| TCTGCCACAGTGCTTAGGAG+AGG | 0.635941 | 3.2:+65291746 | MS.gene99887:intron |
| AATAGTGATGCAGAAGACGA+TGG | 0.643756 | 3.2:+65292700 | MS.gene99887:CDS |
| TCCTGTGTACAGTAAACGTG+TGG | 0.644428 | 3.2:+65293018 | MS.gene99887:CDS |
| AAGATTCCAGTGGAAACTAG+TGG | 0.653175 | 3.2:+65294149 | MS.gene99887:CDS |
| GATGAGAAGTGTACTTACGT+AGG | 0.655130 | 3.2:+65299016 | MS.gene99887:CDS |
| ACTAGTGGCAAAGATACCAA+AGG | 0.657324 | 3.2:+65294164 | MS.gene99887:CDS |
| TCTGCTTAAAGAACAAAAGG+GGG | 0.658439 | 3.2:+65291926 | MS.gene99887:CDS |
| TGATGCCACAAAGACGTCTG+AGG | 0.660915 | 3.2:+65291782 | MS.gene99887:CDS |
| ACCAAAGCCCAAGATTCCAG+TGG | 0.665045 | 3.2:+65294139 | MS.gene99887:CDS |
| GAAAGTACACAAGAGACTGA+AGG | 0.670007 | 3.2:+65291822 | MS.gene99887:CDS |
| TCCACACGTTTACTGTACAC+AGG | 0.676303 | 3.2:-65293019 | None:intergenic |
| AATCTGTTATTAAAGCATGT+GGG | 0.677116 | 3.2:+65293050 | MS.gene99887:CDS |
| CTTCCTGAAACCATTAATGG+TGG | 0.690504 | 3.2:+65298944 | MS.gene99887:CDS |
| GTCAGCAGAGAATACTACCA+AGG | 0.702422 | 3.2:+65292753 | MS.gene99887:CDS |
| GCACGGGCCAAGGAACTCGA+GGG | 0.706558 | 3.2:+65294047 | MS.gene99887:CDS |
| CTGCTTAAAGAACAAAAGGG+GGG | 0.713202 | 3.2:+65291927 | MS.gene99887:CDS |
| AGAAACGCGAGAGCGCATGG+CGG | 0.788364 | 3.2:-65290335 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 65290271 | 65300637 | 65290271 | ID=MS.gene99887 |
| chr3.2 | mRNA | 65290271 | 65300637 | 65290271 | ID=MS.gene99887.t1;Parent=MS.gene99887 |
| chr3.2 | exon | 65290271 | 65290377 | 65290271 | ID=MS.gene99887.t1.exon1;Parent=MS.gene99887.t1 |
| chr3.2 | CDS | 65290271 | 65290377 | 65290271 | ID=cds.MS.gene99887.t1;Parent=MS.gene99887.t1 |
| chr3.2 | exon | 65290471 | 65290576 | 65290471 | ID=MS.gene99887.t1.exon2;Parent=MS.gene99887.t1 |
| chr3.2 | CDS | 65290471 | 65290576 | 65290471 | ID=cds.MS.gene99887.t1;Parent=MS.gene99887.t1 |
| chr3.2 | exon | 65291756 | 65292060 | 65291756 | ID=MS.gene99887.t1.exon3;Parent=MS.gene99887.t1 |
| chr3.2 | CDS | 65291756 | 65292060 | 65291756 | ID=cds.MS.gene99887.t1;Parent=MS.gene99887.t1 |
| chr3.2 | exon | 65292222 | 65292327 | 65292222 | ID=MS.gene99887.t1.exon4;Parent=MS.gene99887.t1 |
| chr3.2 | CDS | 65292222 | 65292327 | 65292222 | ID=cds.MS.gene99887.t1;Parent=MS.gene99887.t1 |
| chr3.2 | exon | 65292424 | 65292774 | 65292424 | ID=MS.gene99887.t1.exon5;Parent=MS.gene99887.t1 |
| chr3.2 | CDS | 65292424 | 65292774 | 65292424 | ID=cds.MS.gene99887.t1;Parent=MS.gene99887.t1 |
| chr3.2 | exon | 65292995 | 65293077 | 65292995 | ID=MS.gene99887.t1.exon6;Parent=MS.gene99887.t1 |
| chr3.2 | CDS | 65292995 | 65293077 | 65292995 | ID=cds.MS.gene99887.t1;Parent=MS.gene99887.t1 |
| chr3.2 | exon | 65293157 | 65293281 | 65293157 | ID=MS.gene99887.t1.exon7;Parent=MS.gene99887.t1 |
| chr3.2 | CDS | 65293157 | 65293281 | 65293157 | ID=cds.MS.gene99887.t1;Parent=MS.gene99887.t1 |
| chr3.2 | exon | 65294021 | 65294185 | 65294021 | ID=MS.gene99887.t1.exon8;Parent=MS.gene99887.t1 |
| chr3.2 | CDS | 65294021 | 65294185 | 65294021 | ID=cds.MS.gene99887.t1;Parent=MS.gene99887.t1 |
| chr3.2 | exon | 65298891 | 65299084 | 65298891 | ID=MS.gene99887.t1.exon9;Parent=MS.gene99887.t1 |
| chr3.2 | CDS | 65298891 | 65299084 | 65298891 | ID=cds.MS.gene99887.t1;Parent=MS.gene99887.t1 |
| chr3.2 | exon | 65299188 | 65299245 | 65299188 | ID=MS.gene99887.t1.exon10;Parent=MS.gene99887.t1 |
| chr3.2 | CDS | 65299188 | 65299245 | 65299188 | ID=cds.MS.gene99887.t1;Parent=MS.gene99887.t1 |
| chr3.2 | exon | 65299333 | 65299439 | 65299333 | ID=MS.gene99887.t1.exon11;Parent=MS.gene99887.t1 |
| chr3.2 | CDS | 65299333 | 65299439 | 65299333 | ID=cds.MS.gene99887.t1;Parent=MS.gene99887.t1 |
| chr3.2 | exon | 65299716 | 65299758 | 65299716 | ID=MS.gene99887.t1.exon12;Parent=MS.gene99887.t1 |
| chr3.2 | CDS | 65299716 | 65299758 | 65299716 | ID=cds.MS.gene99887.t1;Parent=MS.gene99887.t1 |
| chr3.2 | exon | 65300498 | 65300637 | 65300498 | ID=MS.gene99887.t1.exon13;Parent=MS.gene99887.t1 |
| chr3.2 | CDS | 65300498 | 65300637 | 65300498 | ID=cds.MS.gene99887.t1;Parent=MS.gene99887.t1 |
| Gene Sequence |
| Protein sequence |
