AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061333


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 52 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAAGCATTATCAGAATATT+TGG 0.154201 8.1:-87229372 MS.gene061333:CDS
CTTGATACTGACATTGTTCT+AGG 0.157660 8.1:+87229593 None:intergenic
ATTAACTTTGTTACTGTTCT+TGG 0.162375 8.1:+87229818 None:intergenic
ACCATTTCAAAGCGCGTCTA+AGG 0.318480 8.1:-87229673 MS.gene061333:CDS
ACGTGAAACTAAGCTTGTTT+TGG 0.326591 8.1:-87230053 MS.gene061333:CDS
GACTTTGTAGTTCTTGATCA+TGG 0.348123 8.1:+87230483 None:intergenic
TCTTTATTTCCAAGGTGAAT+TGG 0.350401 8.1:+87230583 None:intergenic
ATCAAATCAGTGAAATGTTT+AGG 0.353279 8.1:+87229905 None:intergenic
CTGCTAAACTTAACTCTTCA+AGG 0.360388 8.1:+87230686 None:intergenic
TTACCTTCCAGCTGATTTAA+CGG 0.361070 8.1:-87229873 MS.gene061333:CDS
AACATTTCACTGATTTGATA+AGG 0.390830 8.1:-87229901 MS.gene061333:CDS
ACTCCTCAATTAATCTCAAT+TGG 0.392658 8.1:-87230607 MS.gene061333:CDS
GTAACAAAGTTAATAGATTC+TGG 0.396787 8.1:-87229809 MS.gene061333:CDS
AAGAAACCGTTCATGGAAAT+CGG 0.400354 8.1:-87230338 MS.gene061333:CDS
GAAGTGGCAGTGCTTGTTAA+TGG 0.401978 8.1:-87229454 MS.gene061333:CDS
ATAGACGCTCATAGAAGAAA+TGG 0.406927 8.1:+87230524 None:intergenic
TCCTTAGACGCGCTTTGAAA+TGG 0.415205 8.1:+87229672 None:intergenic
TCTAAATCCGTTAAATCAGC+TGG 0.442093 8.1:+87229866 None:intergenic
AGCAATAACCATAGGTTTCA+AGG 0.443493 8.1:-87229929 MS.gene061333:CDS
CAGAGAAAATAACTCAGAAA+TGG 0.447228 8.1:+87229728 None:intergenic
ATCTGAAACTGAATATGAAA+TGG 0.454211 8.1:-87230734 MS.gene061333:CDS
GTTTAGGACCTTGAAACCTA+TGG 0.455366 8.1:+87229921 None:intergenic
AGAGCTAGTTCTACTTTGGT+TGG 0.457619 8.1:-87229301 MS.gene061333:CDS
GGAAGTGATTGTGCATGAAA+TGG 0.461128 8.1:-87229488 MS.gene061333:CDS
GGACCAATTGAGATTAATTG+AGG 0.464532 8.1:+87230604 None:intergenic
TTGGAGAGCTAGTTCTACTT+TGG 0.466290 8.1:-87229305 MS.gene061333:CDS
AGTGGCTTAATTGGTGATGT+TGG 0.493716 8.1:-87229998 MS.gene061333:CDS
GTTGAAGTTCTTTATTTCCA+AGG 0.495312 8.1:+87230575 None:intergenic
TCTAAGGATTCCGCAGTTGA+AGG 0.499213 8.1:-87229657 MS.gene061333:CDS
AGCAGATTGTAGCATCTACA+AGG 0.504878 8.1:-87230668 MS.gene061333:CDS
ATTACATTCTAACACAAGCT+TGG 0.511928 8.1:-87230252 MS.gene061333:CDS
TTGATACTGACATTGTTCTA+GGG 0.522905 8.1:+87229594 None:intergenic
ATGTTTCAATCTAAATGACT+TGG 0.523292 8.1:+87230282 None:intergenic
GAAGTGATTGTGCATGAAAT+GGG 0.523677 8.1:-87229487 MS.gene061333:CDS
TTAACGGATTTAGAAAAGAG+TGG 0.543465 8.1:-87229857 MS.gene061333:CDS
CACCAACCACAATGCAGCCA+TGG 0.546124 8.1:+87229342 None:intergenic
TGGTTCAAACATGAAAATGA+AGG 0.548134 8.1:+87229692 None:intergenic
TCAATTGGTCCAATTCACCT+TGG 0.558480 8.1:-87230592 MS.gene061333:CDS
TTAATTGGTGATGTTGGTAG+TGG 0.564871 8.1:-87229992 MS.gene061333:CDS
ACCACAATGCAGCCATGGTA+CGG 0.571593 8.1:+87229347 None:intergenic
ATGTATCCGATTTCCATGAA+CGG 0.580945 8.1:+87230332 None:intergenic
TGGTCTTTGTAAACATGTTG+TGG 0.582048 8.1:-87229434 MS.gene061333:CDS
AATCTATGAGCAATAACCAT+AGG 0.583238 8.1:-87229937 MS.gene061333:CDS
GACATTGTTCTAGGGCAATG+AGG 0.593451 8.1:+87229602 None:intergenic
GGCTCAGCAAAAGCATCACA+AGG 0.605591 8.1:-87230231 MS.gene061333:CDS
TACCATGGCTGCATTGTGGT+TGG 0.611964 8.1:-87229344 MS.gene061333:CDS
AATCCGTTAAATCAGCTGGA+AGG 0.622325 8.1:+87229870 None:intergenic
AATGGGAAGTGATAAAGAAG+TGG 0.638138 8.1:-87229470 MS.gene061333:CDS
GAATATTTGGAACCGTACCA+TGG 0.643141 8.1:-87229359 MS.gene061333:CDS
GTGTGATCAACCTTCAACTG+CGG 0.710202 8.1:+87229647 None:intergenic
GCTCAGCAAAAGCATCACAA+GGG 0.717992 8.1:-87230230 MS.gene061333:CDS
ACCGTACCATGGCTGCATTG+TGG 0.722473 8.1:-87229348 MS.gene061333:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAGCATTATCAGAATATT+TGG - chr8.1:87230648-87230667 MS.gene061333:CDS 20.0%
!! GAAACATAATTGCATATATA+AGG + chr8.1:87230458-87230477 None:intergenic 20.0%
!!! AAATTTTTCTTTGCTTATGT+TGG + chr8.1:87229615-87229634 None:intergenic 20.0%
!!! AAGAAATATTATTTTGTGAG+TGG - chr8.1:87230004-87230023 MS.gene061333:CDS 20.0%
!!! AATTTTTCTTTGCTTATGTT+GGG + chr8.1:87229614-87229633 None:intergenic 20.0%
!!! TTTATCAGAATGTTTTTTCT+TGG + chr8.1:87230065-87230084 None:intergenic 20.0%
! ATCAAATCAGTGAAATGTTT+AGG + chr8.1:87230118-87230137 None:intergenic 25.0%
! ATGTTTCAATCTAAATGACT+TGG + chr8.1:87229741-87229760 None:intergenic 25.0%
! ATTAACTTTGTTACTGTTCT+TGG + chr8.1:87230205-87230224 None:intergenic 25.0%
! CATAAGCAAAGAAAAATTTG+TGG - chr8.1:87229616-87229635 MS.gene061333:CDS 25.0%
! GTAACAAAGTTAATAGATTC+TGG - chr8.1:87230211-87230230 MS.gene061333:CDS 25.0%
!! ATCTGAAACTGAATATGAAA+TGG - chr8.1:87229286-87229305 MS.gene061333:CDS 25.0%
!! ATTTGTGGAAACAATTTTGT+TGG - chr8.1:87229631-87229650 MS.gene061333:CDS 25.0%
!! CAAGATCAAGAAAACTTTTA+TGG + chr8.1:87229904-87229923 None:intergenic 25.0%
!!! AACATTTCACTGATTTGATA+AGG - chr8.1:87230119-87230138 MS.gene061333:CDS 25.0%
AATCAACATCAACTTGTGTA+TGG + chr8.1:87230499-87230518 None:intergenic 30.0%
AATCTATGAGCAATAACCAT+AGG - chr8.1:87230083-87230102 MS.gene061333:CDS 30.0%
ACTCCTCAATTAATCTCAAT+TGG - chr8.1:87229413-87229432 MS.gene061333:CDS 30.0%
AGATTCATCAGCACAAAAAA+AGG + chr8.1:87229842-87229861 None:intergenic 30.0%
ATTACATTCTAACACAAGCT+TGG - chr8.1:87229768-87229787 MS.gene061333:CDS 30.0%
CAATGCAACAACACAAAAAA+AGG - chr8.1:87229468-87229487 MS.gene061333:CDS 30.0%
CAGAGAAAATAACTCAGAAA+TGG + chr8.1:87230295-87230314 None:intergenic 30.0%
TGGTTCAAACATGAAAATGA+AGG + chr8.1:87230331-87230350 None:intergenic 30.0%
TTGATACTGACATTGTTCTA+GGG + chr8.1:87230429-87230448 None:intergenic 30.0%
! GTTGAAGTTCTTTATTTCCA+AGG + chr8.1:87229448-87229467 None:intergenic 30.0%
! TATTTTGTGAGTGGCTTAAT+TGG - chr8.1:87230013-87230032 MS.gene061333:CDS 30.0%
! TCTTTATTTCCAAGGTGAAT+TGG + chr8.1:87229440-87229459 None:intergenic 30.0%
! TTTTGTTAAGAAACCGTTCA+TGG - chr8.1:87229675-87229694 MS.gene061333:CDS 30.0%
!! AAGCTTCTTGTTTCACTTTT+CGG + chr8.1:87229391-87229410 None:intergenic 30.0%
!! ACAAGTTGATGTTGATTTGT+TGG - chr8.1:87230502-87230521 MS.gene061333:CDS 30.0%
!! CACAACAGAATGTGTTTTTA+AGG - chr8.1:87230393-87230412 MS.gene061333:CDS 30.0%
!! TTAACGGATTTAGAAAAGAG+TGG - chr8.1:87230163-87230182 MS.gene061333:CDS 30.0%
!!! CTTTTTTTGTGTTGTTGCAT+TGG + chr8.1:87229470-87229489 None:intergenic 30.0%
AAGAAACCGTTCATGGAAAT+CGG - chr8.1:87229682-87229701 MS.gene061333:CDS 35.0%
AATGGGAAGTGATAAAGAAG+TGG - chr8.1:87230550-87230569 MS.gene061333:CDS 35.0%
AGCAATAACCATAGGTTTCA+AGG - chr8.1:87230091-87230110 MS.gene061333:CDS 35.0%
ATAGACGCTCATAGAAGAAA+TGG + chr8.1:87229499-87229518 None:intergenic 35.0%
ATGTATCCGATTTCCATGAA+CGG + chr8.1:87229691-87229710 None:intergenic 35.0%
CTGCTAAACTTAACTCTTCA+AGG + chr8.1:87229337-87229356 None:intergenic 35.0%
CTTGATACTGACATTGTTCT+AGG + chr8.1:87230430-87230449 None:intergenic 35.0%
CTTGCTAGAATTCTCTTCTA+AGG - chr8.1:87230782-87230801 MS.gene061333:CDS 35.0%
GAAGTGATTGTGCATGAAAT+GGG - chr8.1:87230533-87230552 MS.gene061333:CDS 35.0%
GACTTTGTAGTTCTTGATCA+TGG + chr8.1:87229540-87229559 None:intergenic 35.0%
GGACCAATTGAGATTAATTG+AGG + chr8.1:87229419-87229438 None:intergenic 35.0%
TCTAAATCCGTTAAATCAGC+TGG + chr8.1:87230157-87230176 None:intergenic 35.0%
TGGTCTTTGTAAACATGTTG+TGG - chr8.1:87230586-87230605 MS.gene061333:CDS 35.0%
! TTACCTTCCAGCTGATTTAA+CGG - chr8.1:87230147-87230166 MS.gene061333:CDS 35.0%
!! ACGTGAAACTAAGCTTGTTT+TGG - chr8.1:87229967-87229986 MS.gene061333:CDS 35.0%
!! CTGAGTTATTTTCTCTGCTT+AGG - chr8.1:87230298-87230317 MS.gene061333:CDS 35.0%
!! TGGTGTATTTTAGAGATGCT+TGG - chr8.1:87230696-87230715 MS.gene061333:CDS 35.0%
!! TTAATTGGTGATGTTGGTAG+TGG - chr8.1:87230028-87230047 MS.gene061333:CDS 35.0%
!!! CTTCTTTTTTGTCGTCGTTA+GGG + chr8.1:87229880-87229899 None:intergenic 35.0%
!!! GTGAGTTTTGAGAAAGTTGT+GGG - chr8.1:87230235-87230254 MS.gene061333:CDS 35.0%
!!! TCTTCTTTTTTGTCGTCGTT+AGG + chr8.1:87229881-87229900 None:intergenic 35.0%
!!! TGAGTTTTGAGAAAGTTGTG+GGG - chr8.1:87230236-87230255 MS.gene061333:CDS 35.0%
!!! TGTGAGTTTTGAGAAAGTTG+TGG - chr8.1:87230234-87230253 MS.gene061333:CDS 35.0%
AATCCGTTAAATCAGCTGGA+AGG + chr8.1:87230153-87230172 None:intergenic 40.0%
AGCAGATTGTAGCATCTACA+AGG - chr8.1:87229352-87229371 MS.gene061333:CDS 40.0%
GGAAGTGATTGTGCATGAAA+TGG - chr8.1:87230532-87230551 MS.gene061333:CDS 40.0%
TCAATTGGTCCAATTCACCT+TGG - chr8.1:87229428-87229447 MS.gene061333:CDS 40.0%
! CACAAGGGACTTGATTTTGT+TGG - chr8.1:87229805-87229824 MS.gene061333:CDS 40.0%
! GAATATTTGGAACCGTACCA+TGG - chr8.1:87230661-87230680 MS.gene061333:CDS 40.0%
! GTTTAGGACCTTGAAACCTA+TGG + chr8.1:87230102-87230121 None:intergenic 40.0%
!! AGAGCTAGTTCTACTTTGGT+TGG - chr8.1:87230719-87230738 MS.gene061333:CDS 40.0%
!! AGTGGCTTAATTGGTGATGT+TGG - chr8.1:87230022-87230041 MS.gene061333:CDS 40.0%
!! TGTTGATTTGTTGGTCGAGA+AGG - chr8.1:87230511-87230530 MS.gene061333:CDS 40.0%
!! TTCACTTTTCGGAGATTGTG+AGG + chr8.1:87229380-87229399 None:intergenic 40.0%
!! TTGGAGAGCTAGTTCTACTT+TGG - chr8.1:87230715-87230734 MS.gene061333:CDS 40.0%
!!! GTTGGATGCTGTTTTCATCA+TGG - chr8.1:87229649-87229668 MS.gene061333:CDS 40.0%
ACCATTTCAAAGCGCGTCTA+AGG - chr8.1:87230347-87230366 MS.gene061333:CDS 45.0%
GTGTGATCAACCTTCAACTG+CGG + chr8.1:87230376-87230395 None:intergenic 45.0%
TCTAAGGATTCCGCAGTTGA+AGG - chr8.1:87230363-87230382 MS.gene061333:CDS 45.0%
! GAAGTGGCAGTGCTTGTTAA+TGG - chr8.1:87230566-87230585 MS.gene061333:CDS 45.0%
!! GACATTGTTCTAGGGCAATG+AGG + chr8.1:87230421-87230440 None:intergenic 45.0%
!! GCTCAGCAAAAGCATCACAA+GGG - chr8.1:87229790-87229809 MS.gene061333:CDS 45.0%
!! TCCTTAGACGCGCTTTGAAA+TGG + chr8.1:87230351-87230370 None:intergenic 45.0%
ACCACAATGCAGCCATGGTA+CGG + chr8.1:87230676-87230695 None:intergenic 50.0%
TACCATGGCTGCATTGTGGT+TGG - chr8.1:87230676-87230695 MS.gene061333:CDS 50.0%
!! GGCTCAGCAAAAGCATCACA+AGG - chr8.1:87229789-87229808 MS.gene061333:CDS 50.0%
ACCGTACCATGGCTGCATTG+TGG - chr8.1:87230672-87230691 MS.gene061333:CDS 55.0%
CACCAACCACAATGCAGCCA+TGG + chr8.1:87230681-87230700 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 87229240 87230802 87229240 ID=MS.gene061333
chr8.1 mRNA 87229240 87230802 87229240 ID=MS.gene061333.t1;Parent=MS.gene061333
chr8.1 exon 87229601 87230802 87229601 ID=MS.gene061333.t1.exon1;Parent=MS.gene061333.t1
chr8.1 CDS 87229601 87230802 87229601 ID=cds.MS.gene061333.t1;Parent=MS.gene061333.t1
chr8.1 exon 87229240 87229509 87229240 ID=MS.gene061333.t1.exon2;Parent=MS.gene061333.t1
chr8.1 CDS 87229240 87229509 87229240 ID=cds.MS.gene061333.t1;Parent=MS.gene061333.t1
Gene Sequence

>MS.gene061333

GGAAAAATAATATTATGGCACATGCTAATACTTCTGTTGATGCTTTATCTGAAACTGAATATGAAATGGTGAAACACATCATTGACGTTGAAGTCCTTGAAGAGTTAAGTTTAGCAGATTGTAGCATCTACAAGGTTCCTCACAATCTCCGAAAAGTGAAACAAGAAGCTTACACTCCTCAATTAATCTCAATTGGTCCAATTCACCTTGGAAATAAAGAACTTCAACCAATGCAACAACACAAAAAAAGGTATTTCCATTTCTTCTATGAGCGTCTATCTCAATTCAATCAACAATCCATGATCAAGAACTACAAAGTCTATCTTGAAAACAAAGAACAAGAAATAAGAAAATGTTATGCAGAGAAATTTCCCAACATAAGCAAAGAAAAATTTGTGGAAACAATTTTGTTGGATGCTGTTTTCATCATGGAGCTTTTGTTAAGAAACCGTTCATGGAAATCGGATACATCAAAACATGAACATGAATACAAGCAATCCAAGTCATTTAGATTGAAACATAGTGATGATTACATTCTAACACAAGCTTGGCTCAGCAAAAGCATCACAAGGGACTTGATTTTGTTGGAAAATCAGATACCTTTTTTTGTGCTGATGAATCTTTACGATACTGTCGTCCCTAACGACGACAAAAAAGAAGACCATAAAAGTTTTCTTGATCTTGCACTTGAGTATTTTGCATTCTACGACACTCAGAAATCGTCATCACGTGAAACTAAGCTTGTTTTGGATAAGAATCAGACAAAGAAATATTATTTTGTGAGTGGCTTAATTGGTGATGTTGGTAGTGGTAATTCAGTTTCCAAGAAAAAACATTCTGATAAATCTATGAGCAATAACCATAGGTTTCAAGGTCCTAAACATTTCACTGATTTGATAAGGTTTTTTTACCTTCCAGCTGATTTAACGGATTTAGAAAAGAGTGGTTGTTCTCATACTTTTCCAAGAACAGTAACAAAGTTAATAGATTCTGGTGTGAGTTTTGAGAAAGTTGTGGGGAGAAGATTGCTAGAAATAACATTTGAGAAGAAACCATTTCTGAGTTATTTTCTCTGCTTAGGTTGTTTACCTTCATTTTCATGTTTGAACCATTTCAAAGCGCGTCTAAGGATTCCGCAGTTGAAGGTTGATCACACAACAGAATGTGTTTTTAAGGAACCTCATTGCCCTAGAACAATGTCAGTATCAAGAACAACCTTATATATGCAATTATGTTTCACTTGTTGATTCTCTTATCCATACACAAGTTGATGTTGATTTGTTGGTCGAGAAGGAAGTGATTGTGCATGAAATGGGAAGTGATAAAGAAGTGGCAGTGCTTGTTAATGGTCTTTGTAAACATGTTGTGGTGAATTCAACTTGTTACTATGATATTATTAATGAGCTTAATAAGCATTATCAGAATATTTGGAACCGTACCATGGCTGCATTGTGGTTGGTGTATTTTAGAGATGCTTGGAGAGCTAGTTCTACTTTGGTTGGAATTGCTTTTCTTGTTTACACAGCATTCAACTTTGTACGTCTTGCTAGAATTCTCTTCTAA

Protein sequence

>MS.gene061333.t1

GKIILWHMLILLLMLYLKLNMKWUNTSLTLKSLKSUVUQIVASTRFLTISEKUNKKLTLLNUSQLVQFTLEIKNFNQCNNTKKGISISSMSVYLNSINNPUSRTTKSILKTKNKKUENVMQRNFPTUAKKNLWKQFCWMLFSSWSFCUETVHGNRIHQNMNMNTSNPSHLDUNIVMITFUHKLGSAKASQGTUFCWKIRYLFLCUUIFTILSSLTTTKKKTIKVFLILHLSILHSTTLRNRHHVKLSLFWIRIRQRNIILUVAULVMLVVVIQFPRKNILINLUAITIGFKVLNISLIUUGFFTFQLIURIUKRVVVLILFQEQUQSUUILVUVLRKLWGEDCUKUHLRRNHFUVIFSAUVVYLHFHVUTISKRVUGFRSURLITQQNVFLRNLIALEQCQKULCMKWEVIKKWQCLLMVFVNMLWUIQLVTMILLMSLISIIRIFGTVPWLHCGWCILEMLGELVLLWLELLFLFTQHSTLYVLLEFSS