Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062572.t1 | XP_003606407.1 | 93.1 | 218 | 15 | 0 | 1 | 218 | 1 | 218 | 1.90E-117 | 431.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062572.t1 | Q9SR36 | 45.6 | 215 | 113 | 3 | 3 | 215 | 5 | 217 | 3.6e-48 | 193.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062572.t1 | G7JPE9 | 93.1 | 218 | 15 | 0 | 1 | 218 | 1 | 218 | 1.4e-117 | 431.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene062572 | -0.800157 | 1.64E-48 | -1.69E-46 |
| MS.gene049611 | MS.gene062572 | 0.82612 | 3.23E-54 | -1.69E-46 |
| MS.gene050082 | MS.gene062572 | 0.816994 | 4.15E-52 | -1.69E-46 |
| MS.gene050323 | MS.gene062572 | -0.833839 | 4.23E-56 | -1.69E-46 |
| MS.gene050381 | MS.gene062572 | 0.813182 | 2.91E-51 | -1.69E-46 |
| MS.gene050444 | MS.gene062572 | -0.80843 | 3.11E-50 | -1.69E-46 |
| MS.gene050612 | MS.gene062572 | -0.803911 | 2.78E-49 | -1.69E-46 |
| MS.gene05079 | MS.gene062572 | 0.815347 | 9.69E-52 | -1.69E-46 |
| MS.gene050809 | MS.gene062572 | 0.832101 | 1.15E-55 | -1.69E-46 |
| MS.gene050832 | MS.gene062572 | 0.801545 | 8.56E-49 | -1.69E-46 |
| MS.gene050833 | MS.gene062572 | 0.807265 | 5.50E-50 | -1.69E-46 |
| MS.gene05104 | MS.gene062572 | 0.814203 | 1.74E-51 | -1.69E-46 |
| MS.gene051127 | MS.gene062572 | 0.818101 | 2.34E-52 | -1.69E-46 |
| MS.gene051138 | MS.gene062572 | 0.850014 | 2.23E-60 | -1.69E-46 |
| MS.gene051364 | MS.gene062572 | 0.822151 | 2.76E-53 | -1.69E-46 |
| MS.gene051366 | MS.gene062572 | 0.834001 | 3.86E-56 | -1.69E-46 |
| MS.gene051422 | MS.gene062572 | 0.827092 | 1.89E-54 | -1.69E-46 |
| MS.gene051651 | MS.gene062572 | 0.835799 | 1.36E-56 | -1.69E-46 |
| MS.gene051863 | MS.gene062572 | 0.816798 | 4.59E-52 | -1.69E-46 |
| MS.gene052129 | MS.gene062572 | 0.811766 | 5.94E-51 | -1.69E-46 |
| MS.gene053420 | MS.gene062572 | 0.841763 | 3.90E-58 | -1.69E-46 |
| MS.gene053887 | MS.gene062572 | 0.802477 | 5.51E-49 | -1.69E-46 |
| MS.gene054706 | MS.gene062572 | 0.829146 | 6.06E-55 | -1.69E-46 |
| MS.gene055154 | MS.gene062572 | 0.817737 | 2.82E-52 | -1.69E-46 |
| MS.gene055202 | MS.gene062572 | 0.808755 | 2.65E-50 | -1.69E-46 |
| MS.gene055502 | MS.gene062572 | -0.809559 | 1.78E-50 | -1.69E-46 |
| MS.gene05589 | MS.gene062572 | -0.816143 | 6.44E-52 | -1.69E-46 |
| MS.gene055942 | MS.gene062572 | 0.81133 | 7.39E-51 | -1.69E-46 |
| MS.gene056224 | MS.gene062572 | 0.824987 | 5.99E-54 | -1.69E-46 |
| MS.gene056387 | MS.gene062572 | -0.800999 | 1.11E-48 | -1.69E-46 |
| MS.gene056504 | MS.gene062572 | -0.824419 | 8.15E-54 | -1.69E-46 |
| MS.gene056856 | MS.gene062572 | 0.871048 | 9.20E-67 | -1.69E-46 |
| MS.gene056857 | MS.gene062572 | 0.855985 | 4.37E-62 | -1.69E-46 |
| MS.gene057473 | MS.gene062572 | 0.807383 | 5.19E-50 | -1.69E-46 |
| MS.gene057547 | MS.gene062572 | 0.820727 | 5.89E-53 | -1.69E-46 |
| MS.gene057548 | MS.gene062572 | 0.821475 | 3.96E-53 | -1.69E-46 |
| MS.gene057549 | MS.gene062572 | 0.850472 | 1.66E-60 | -1.69E-46 |
| MS.gene057768 | MS.gene062572 | 0.818967 | 1.49E-52 | -1.69E-46 |
| MS.gene058067 | MS.gene062572 | 0.828503 | 8.67E-55 | -1.69E-46 |
| MS.gene058068 | MS.gene062572 | 0.82609 | 3.28E-54 | -1.69E-46 |
| MS.gene058093 | MS.gene062572 | 0.809368 | 1.96E-50 | -1.69E-46 |
| MS.gene058099 | MS.gene062572 | 0.842424 | 2.61E-58 | -1.69E-46 |
| MS.gene058111 | MS.gene062572 | 0.81423 | 1.71E-51 | -1.69E-46 |
| MS.gene058171 | MS.gene062572 | 0.80149 | 8.79E-49 | -1.69E-46 |
| MS.gene058175 | MS.gene062572 | 0.811795 | 5.86E-51 | -1.69E-46 |
| MS.gene058268 | MS.gene062572 | 0.831587 | 1.53E-55 | -1.69E-46 |
| MS.gene058269 | MS.gene062572 | 0.826128 | 3.21E-54 | -1.69E-46 |
| MS.gene058334 | MS.gene062572 | 0.809751 | 1.62E-50 | -1.69E-46 |
| MS.gene058382 | MS.gene062572 | 0.817926 | 2.56E-52 | -1.69E-46 |
| MS.gene058603 | MS.gene062572 | 0.818245 | 2.17E-52 | -1.69E-46 |
| MS.gene059069 | MS.gene062572 | 0.806942 | 6.44E-50 | -1.69E-46 |
| MS.gene059070 | MS.gene062572 | 0.807157 | 5.80E-50 | -1.69E-46 |
| MS.gene059699 | MS.gene062572 | 0.808485 | 3.03E-50 | -1.69E-46 |
| MS.gene059703 | MS.gene062572 | 0.81957 | 1.08E-52 | -1.69E-46 |
| MS.gene059865 | MS.gene062572 | -0.816573 | 5.16E-52 | -1.69E-46 |
| MS.gene060405 | MS.gene062572 | 0.814682 | 1.36E-51 | -1.69E-46 |
| MS.gene060762 | MS.gene062572 | -0.807653 | 4.55E-50 | -1.69E-46 |
| MS.gene060823 | MS.gene062572 | 0.827039 | 1.95E-54 | -1.69E-46 |
| MS.gene060982 | MS.gene062572 | -0.802295 | 6.00E-49 | -1.69E-46 |
| MS.gene061044 | MS.gene062572 | 0.818042 | 2.41E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062572.t1 | MTR_4g059730 | 93.119 | 218 | 15 | 0 | 1 | 218 | 1 | 218 | 5.75e-152 | 421 |
| MS.gene062572.t1 | MTR_1g090060 | 68.372 | 215 | 68 | 0 | 4 | 218 | 3 | 217 | 2.20e-111 | 318 |
| MS.gene062572.t1 | MTR_1g090100 | 67.290 | 214 | 70 | 0 | 5 | 218 | 3 | 216 | 2.93e-106 | 305 |
| MS.gene062572.t1 | MTR_1g090070 | 63.426 | 216 | 78 | 1 | 4 | 218 | 9 | 224 | 2.35e-102 | 295 |
| MS.gene062572.t1 | MTR_1g090090 | 65.581 | 215 | 74 | 0 | 4 | 218 | 3 | 217 | 4.04e-102 | 295 |
| MS.gene062572.t1 | MTR_1g115195 | 48.837 | 215 | 106 | 3 | 1 | 213 | 1 | 213 | 2.92e-66 | 204 |
| MS.gene062572.t1 | MTR_1g090150 | 47.867 | 211 | 109 | 1 | 3 | 213 | 2 | 211 | 5.53e-66 | 203 |
| MS.gene062572.t1 | MTR_4g019790 | 45.755 | 212 | 111 | 2 | 4 | 214 | 7 | 215 | 2.04e-62 | 194 |
| MS.gene062572.t1 | MTR_4g019780 | 46.083 | 217 | 113 | 3 | 3 | 218 | 7 | 220 | 7.06e-61 | 190 |
| MS.gene062572.t1 | MTR_7g065660 | 46.479 | 213 | 110 | 2 | 3 | 214 | 6 | 215 | 3.25e-59 | 186 |
| MS.gene062572.t1 | MTR_7g065740 | 44.393 | 214 | 114 | 2 | 3 | 214 | 6 | 216 | 4.22e-59 | 186 |
| MS.gene062572.t1 | MTR_7g065230 | 45.370 | 216 | 109 | 3 | 3 | 217 | 6 | 213 | 3.60e-58 | 183 |
| MS.gene062572.t1 | MTR_5g040430 | 44.545 | 220 | 116 | 4 | 2 | 216 | 5 | 223 | 2.46e-57 | 181 |
| MS.gene062572.t1 | MTR_7g065720 | 44.393 | 214 | 114 | 2 | 3 | 214 | 6 | 216 | 3.49e-57 | 181 |
| MS.gene062572.t1 | MTR_3g099757 | 45.070 | 213 | 113 | 3 | 3 | 214 | 4 | 213 | 3.08e-56 | 178 |
| MS.gene062572.t1 | MTR_7g065590 | 42.523 | 214 | 117 | 3 | 3 | 214 | 6 | 215 | 7.49e-56 | 177 |
| MS.gene062572.t1 | MTR_8g087425 | 44.651 | 215 | 113 | 2 | 2 | 214 | 3 | 213 | 7.17e-55 | 175 |
| MS.gene062572.t1 | MTR_7g065750 | 42.056 | 214 | 119 | 2 | 3 | 214 | 6 | 216 | 2.07e-54 | 174 |
| MS.gene062572.t1 | MTR_8g087410 | 47.085 | 223 | 107 | 6 | 1 | 218 | 2 | 218 | 2.66e-53 | 171 |
| MS.gene062572.t1 | MTR_2g070120 | 42.723 | 213 | 117 | 3 | 1 | 212 | 1 | 209 | 8.18e-53 | 169 |
| MS.gene062572.t1 | MTR_7g065600 | 39.906 | 213 | 124 | 2 | 3 | 214 | 6 | 215 | 6.55e-52 | 167 |
| MS.gene062572.t1 | MTR_7g065270 | 42.056 | 214 | 118 | 4 | 3 | 214 | 6 | 215 | 8.49e-52 | 167 |
| MS.gene062572.t1 | MTR_2g070150 | 45.455 | 209 | 107 | 4 | 1 | 207 | 1 | 204 | 1.20e-51 | 166 |
| MS.gene062572.t1 | MTR_8g061950 | 43.578 | 218 | 115 | 6 | 5 | 215 | 6 | 222 | 1.14e-50 | 164 |
| MS.gene062572.t1 | MTR_7g065290 | 40.376 | 213 | 123 | 2 | 3 | 214 | 6 | 215 | 3.35e-50 | 163 |
| MS.gene062572.t1 | MTR_6g080440 | 43.434 | 198 | 106 | 3 | 18 | 213 | 17 | 210 | 4.56e-50 | 162 |
| MS.gene062572.t1 | MTR_7g065630 | 40.376 | 213 | 119 | 3 | 3 | 214 | 24 | 229 | 4.85e-50 | 163 |
| MS.gene062572.t1 | MTR_7g065640 | 41.784 | 213 | 102 | 3 | 3 | 214 | 6 | 197 | 5.56e-50 | 162 |
| MS.gene062572.t1 | MTR_7g065710 | 41.395 | 215 | 116 | 4 | 1 | 213 | 1 | 207 | 6.59e-50 | 162 |
| MS.gene062572.t1 | MTR_7g065265 | 40.845 | 213 | 122 | 2 | 3 | 214 | 6 | 215 | 2.93e-49 | 160 |
| MS.gene062572.t1 | MTR_2g070200 | 42.793 | 222 | 118 | 6 | 1 | 218 | 1 | 217 | 3.75e-49 | 160 |
| MS.gene062572.t1 | MTR_7g065700 | 42.056 | 214 | 116 | 3 | 1 | 213 | 4 | 210 | 7.07e-49 | 159 |
| MS.gene062572.t1 | MTR_2g070130 | 40.670 | 209 | 119 | 3 | 1 | 208 | 1 | 205 | 6.32e-48 | 157 |
| MS.gene062572.t1 | MTR_2g070180 | 42.381 | 210 | 114 | 4 | 1 | 208 | 1 | 205 | 9.00e-48 | 156 |
| MS.gene062572.t1 | MTR_7g065260 | 43.256 | 215 | 115 | 5 | 3 | 214 | 13 | 223 | 2.43e-47 | 155 |
| MS.gene062572.t1 | MTR_2g070210 | 40.741 | 216 | 119 | 5 | 1 | 212 | 1 | 211 | 1.51e-46 | 153 |
| MS.gene062572.t1 | MTR_2g070140 | 43.137 | 204 | 109 | 4 | 17 | 218 | 1 | 199 | 4.51e-46 | 152 |
| MS.gene062572.t1 | MTR_7g065680 | 39.906 | 213 | 120 | 3 | 3 | 214 | 6 | 211 | 1.26e-44 | 148 |
| MS.gene062572.t1 | MTR_8g056940 | 39.906 | 213 | 123 | 3 | 1 | 212 | 1 | 209 | 1.90e-44 | 148 |
| MS.gene062572.t1 | MTR_3g467420 | 40.476 | 210 | 120 | 3 | 10 | 218 | 15 | 220 | 1.34e-43 | 146 |
| MS.gene062572.t1 | MTR_5g037380 | 38.222 | 225 | 130 | 4 | 3 | 218 | 4 | 228 | 3.38e-43 | 145 |
| MS.gene062572.t1 | MTR_4g124130 | 40.000 | 220 | 126 | 4 | 1 | 218 | 1 | 216 | 1.49e-42 | 143 |
| MS.gene062572.t1 | MTR_3g467430 | 39.631 | 217 | 126 | 3 | 3 | 218 | 8 | 220 | 1.74e-42 | 143 |
| MS.gene062572.t1 | MTR_2g070060 | 38.426 | 216 | 128 | 3 | 1 | 215 | 1 | 212 | 2.92e-42 | 142 |
| MS.gene062572.t1 | MTR_2g070070 | 40.376 | 213 | 122 | 3 | 1 | 212 | 1 | 209 | 7.38e-42 | 141 |
| MS.gene062572.t1 | MTR_2g070110 | 41.232 | 211 | 114 | 5 | 1 | 208 | 1 | 204 | 2.94e-40 | 137 |
| MS.gene062572.t1 | MTR_5g076900 | 36.321 | 212 | 130 | 3 | 2 | 212 | 4 | 211 | 1.56e-37 | 130 |
| MS.gene062572.t1 | MTR_0186s0030 | 46.324 | 136 | 72 | 1 | 1 | 135 | 1 | 136 | 1.84e-35 | 122 |
| MS.gene062572.t1 | MTR_2g072120 | 60.714 | 56 | 21 | 1 | 48 | 102 | 2 | 57 | 3.01e-19 | 79.0 |
| MS.gene062572.t1 | MTR_5g037390 | 42.857 | 91 | 42 | 2 | 28 | 115 | 11 | 94 | 3.64e-13 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062572.t1 | AT3G09270 | 45.581 | 215 | 113 | 3 | 3 | 215 | 5 | 217 | 6.85e-59 | 185 |
| MS.gene062572.t1 | AT2G29450 | 43.578 | 218 | 119 | 3 | 3 | 218 | 5 | 220 | 3.18e-56 | 178 |
| MS.gene062572.t1 | AT2G29460 | 43.318 | 217 | 120 | 2 | 3 | 218 | 6 | 220 | 6.11e-56 | 177 |
| MS.gene062572.t1 | AT2G29490 | 43.981 | 216 | 118 | 2 | 3 | 217 | 6 | 219 | 1.51e-55 | 176 |
| MS.gene062572.t1 | AT1G10370 | 43.694 | 222 | 120 | 3 | 2 | 218 | 3 | 224 | 2.81e-55 | 176 |
| MS.gene062572.t1 | AT2G29470 | 42.661 | 218 | 121 | 3 | 3 | 218 | 6 | 221 | 9.73e-55 | 174 |
| MS.gene062572.t1 | AT2G29420 | 40.092 | 217 | 128 | 1 | 2 | 218 | 7 | 221 | 3.02e-54 | 173 |
| MS.gene062572.t1 | AT2G29480 | 43.779 | 217 | 119 | 2 | 3 | 218 | 6 | 220 | 7.02e-54 | 172 |
| MS.gene062572.t1 | AT1G17180 | 41.096 | 219 | 124 | 3 | 1 | 218 | 1 | 215 | 4.73e-49 | 160 |
| MS.gene062572.t1 | AT2G29440 | 40.092 | 217 | 127 | 2 | 3 | 218 | 5 | 219 | 1.56e-48 | 159 |
| MS.gene062572.t1 | AT1G78340 | 41.981 | 212 | 118 | 3 | 1 | 211 | 1 | 208 | 2.98e-48 | 157 |
| MS.gene062572.t1 | AT1G10360 | 41.176 | 221 | 125 | 3 | 3 | 218 | 4 | 224 | 4.65e-48 | 157 |
| MS.gene062572.t1 | AT1G27130 | 40.553 | 217 | 123 | 4 | 3 | 213 | 5 | 221 | 7.90e-48 | 157 |
| MS.gene062572.t1 | AT1G74590 | 39.269 | 219 | 130 | 2 | 3 | 218 | 6 | 224 | 3.76e-47 | 155 |
| MS.gene062572.t1 | AT1G78320 | 40.566 | 212 | 117 | 6 | 1 | 209 | 1 | 206 | 6.09e-46 | 152 |
| MS.gene062572.t1 | AT1G78370 | 39.623 | 212 | 123 | 3 | 1 | 211 | 1 | 208 | 1.09e-45 | 151 |
| MS.gene062572.t1 | AT5G62480 | 36.404 | 228 | 130 | 5 | 1 | 217 | 5 | 228 | 1.69e-45 | 151 |
| MS.gene062572.t1 | AT1G69930 | 37.674 | 215 | 130 | 3 | 3 | 213 | 11 | 225 | 4.24e-45 | 150 |
| MS.gene062572.t1 | AT1G59670 | 39.631 | 217 | 125 | 4 | 3 | 213 | 5 | 221 | 1.02e-44 | 149 |
| MS.gene062572.t1 | AT1G27140 | 39.910 | 223 | 127 | 4 | 3 | 218 | 5 | 227 | 1.03e-44 | 149 |
| MS.gene062572.t1 | AT1G78380 | 39.726 | 219 | 127 | 3 | 1 | 218 | 1 | 215 | 1.05e-43 | 146 |
| MS.gene062572.t1 | AT1G17170 | 39.906 | 213 | 122 | 4 | 1 | 212 | 1 | 208 | 4.16e-43 | 144 |
| MS.gene062572.t1 | AT1G59700 | 37.327 | 217 | 130 | 4 | 3 | 213 | 5 | 221 | 2.94e-42 | 143 |
| MS.gene062572.t1 | AT1G17190 | 35.945 | 217 | 134 | 3 | 3 | 218 | 4 | 216 | 2.52e-41 | 140 |
| MS.gene062572.t1 | AT1G69920 | 35.814 | 215 | 132 | 4 | 5 | 213 | 35 | 249 | 2.09e-38 | 134 |
| MS.gene062572.t1 | AT1G78360 | 37.383 | 214 | 128 | 4 | 1 | 212 | 1 | 210 | 2.58e-38 | 132 |
| MS.gene062572.t1 | AT1G53680 | 38.119 | 202 | 118 | 4 | 10 | 208 | 13 | 210 | 5.96e-38 | 131 |
| MS.gene062572.t1 | AT3G43800 | 38.182 | 220 | 127 | 5 | 3 | 217 | 4 | 219 | 2.39e-37 | 130 |
| MS.gene062572.t1 | AT5G62480 | 30.222 | 225 | 117 | 5 | 3 | 217 | 7 | 201 | 2.35e-29 | 109 |
| MS.gene062572.t1 | AT5G62480 | 30.222 | 225 | 118 | 5 | 3 | 217 | 7 | 202 | 3.05e-29 | 108 |
| MS.gene062572.t1 | AT1G78320 | 32.701 | 211 | 108 | 6 | 1 | 209 | 1 | 179 | 2.25e-27 | 103 |
| MS.gene062572.t1 | AT2G02390 | 26.852 | 216 | 137 | 6 | 3 | 206 | 7 | 213 | 3.52e-11 | 60.8 |
Find 37 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGAGCCCAAGCTCGGTTC+TGG | 0.280479 | 4.3:-42221171 | MS.gene062572:CDS |
| AGGAGACACCGTTAACATTC+TGG | 0.299490 | 4.3:-42220715 | MS.gene062572:CDS |
| AAGTGAAGCTACATGGATTT+TGG | 0.302285 | 4.3:-42221429 | MS.gene062572:CDS |
| AATACTACCCTCAAAGTAAA+AGG | 0.302524 | 4.3:+42221400 | None:intergenic |
| AGAATACCATAGCGAAAATC+TGG | 0.331408 | 4.3:-42220794 | MS.gene062572:CDS |
| GTATTGACCCTTAAGCTAAA+AGG | 0.363440 | 4.3:-42221382 | MS.gene062572:CDS |
| TTGAATATATTGATGAGATA+TGG | 0.397859 | 4.3:-42221228 | MS.gene062572:CDS |
| TCTTCTTCTATGTTCTCATA+TGG | 0.416671 | 4.3:+42221355 | None:intergenic |
| AACATTCTGGACATAGCTCT+TGG | 0.422694 | 4.3:-42220702 | MS.gene062572:CDS |
| AAAACTCCGGTGTTGGTTCA+TGG | 0.434187 | 4.3:-42221283 | MS.gene062572:CDS |
| AGCTATGTCCAGAATGTTAA+CGG | 0.440075 | 4.3:+42220707 | None:intergenic |
| GCTATCAGTTTCTCATGATC+AGG | 0.440168 | 4.3:+42220549 | None:intergenic |
| GCTCTCTCATAAGGATCAAC+AGG | 0.442610 | 4.3:+42221187 | None:intergenic |
| CGAGCTTGGGCTCTCTCATA+AGG | 0.459155 | 4.3:+42221178 | None:intergenic |
| AATTCCCTCACTTGCATGCA+TGG | 0.474769 | 4.3:-42220614 | MS.gene062572:CDS |
| TTCAAGAATGTACCTGTCAT+AGG | 0.475029 | 4.3:-42220582 | MS.gene062572:CDS |
| GAGAGCCCAAGCTCGGTTCT+GGG | 0.483641 | 4.3:-42221170 | MS.gene062572:CDS |
| GATTGTATTGAAGAAGTTGA+GGG | 0.534705 | 4.3:+42221317 | None:intergenic |
| TCAAACCATGCATGCAAGTG+AGG | 0.535813 | 4.3:+42220609 | None:intergenic |
| TAAGGATCAACAGGAAGCAA+AGG | 0.547066 | 4.3:+42221196 | None:intergenic |
| AAGGATCAACAGGAAGCAAA+GGG | 0.553758 | 4.3:+42221197 | None:intergenic |
| GCAGATCAAGTGAAGCTACA+TGG | 0.559561 | 4.3:-42221436 | MS.gene062572:CDS |
| GAGGGAATTTCTCAGCTGCT+AGG | 0.567585 | 4.3:+42220628 | None:intergenic |
| CAAACCATGCATGCAAGTGA+GGG | 0.568010 | 4.3:+42220610 | None:intergenic |
| GGATTGTATTGAAGAAGTTG+AGG | 0.571991 | 4.3:+42221316 | None:intergenic |
| GCACAAGAAAACTCCGGTGT+TGG | 0.576241 | 4.3:-42221290 | MS.gene062572:CDS |
| CTAACGAGTGCACCAACTGC+AGG | 0.579048 | 4.3:+42220840 | None:intergenic |
| GAATACCATAGCGAAAATCT+GGG | 0.582158 | 4.3:-42220793 | MS.gene062572:CDS |
| GGTGCACTCGTTAGTAGCGG+TGG | 0.611355 | 4.3:-42220831 | MS.gene062572:CDS |
| CTTATGAGAGAGCCCAAGCT+CGG | 0.617875 | 4.3:-42221177 | MS.gene062572:CDS |
| TTTCCACCATGAACCAACAC+CGG | 0.639081 | 4.3:+42221277 | None:intergenic |
| ATGATCATAGACTCACATAG+AGG | 0.640551 | 4.3:+42221253 | None:intergenic |
| GTAGCGGTGGCGATGAAGAG+CGG | 0.649339 | 4.3:-42220818 | MS.gene062572:CDS |
| TCCAGTGCACAAGAAAACTC+CGG | 0.653047 | 4.3:-42221296 | MS.gene062572:CDS |
| ACTCCGGTGTTGGTTCATGG+TGG | 0.663999 | 4.3:-42221280 | MS.gene062572:CDS |
| GTTGGTGCACTCGTTAGTAG+CGG | 0.664719 | 4.3:-42220834 | MS.gene062572:CDS |
| ACAGGAAGCAAAGGGTACTG+TGG | 0.666663 | 4.3:+42221205 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTAATCAACATATTATTT+AGG | - | chr4.3:42220943-42220962 | MS.gene062572:intron | 10.0% |
| !! | TTATCATATTGAATAAATTT+AGG | + | chr4.3:42221056-42221075 | None:intergenic | 10.0% |
| !! | AAAAATAATATTGTTGGATA+TGG | + | chr4.3:42221020-42221039 | None:intergenic | 15.0% |
| !!! | AAATTACTATTACTTTTTCA+TGG | + | chr4.3:42220880-42220899 | None:intergenic | 15.0% |
| !! | TTGAATATATTGATGAGATA+TGG | - | chr4.3:42220732-42220751 | MS.gene062572:CDS | 20.0% |
| ! | CTAAGAAAATTTGCAAATGA+AGG | + | chr4.3:42220982-42221001 | None:intergenic | 25.0% |
| AATACTACCCTCAAAGTAAA+AGG | + | chr4.3:42220563-42220582 | None:intergenic | 30.0% | |
| GATTGTATTGAAGAAGTTGA+GGG | + | chr4.3:42220646-42220665 | None:intergenic | 30.0% | |
| TCTTCTTCTATGTTCTCATA+TGG | + | chr4.3:42220608-42220627 | None:intergenic | 30.0% | |
| ! | GAAACTGATAGCTTTTATGA+AGG | - | chr4.3:42221422-42221441 | MS.gene062572:CDS | 30.0% |
| ! | GACTACAAGAAGAAACTTTT+TGG | - | chr4.3:42221222-42221241 | MS.gene062572:CDS | 30.0% |
| ! | TACAAGAAGAAACTTTTTGG+AGG | - | chr4.3:42221225-42221244 | MS.gene062572:CDS | 30.0% |
| ! | TTTTCATGGTATGCAAATGA+AGG | + | chr4.3:42220866-42220885 | None:intergenic | 30.0% |
| ! | TTTTTATAGATTCCTGCAGT+TGG | - | chr4.3:42221108-42221127 | MS.gene062572:intron | 30.0% |
| !!! | ATGGTATACCTTTTAGCTTA+AGG | + | chr4.3:42220589-42220608 | None:intergenic | 30.0% |
| !!! | TGGTATACCTTTTAGCTTAA+GGG | + | chr4.3:42220588-42220607 | None:intergenic | 30.0% |
| AGAATACCATAGCGAAAATC+TGG | - | chr4.3:42221166-42221185 | MS.gene062572:CDS | 35.0% | |
| AGCTATGTCCAGAATGTTAA+CGG | + | chr4.3:42221256-42221275 | None:intergenic | 35.0% | |
| ATGATCATAGACTCACATAG+AGG | + | chr4.3:42220710-42220729 | None:intergenic | 35.0% | |
| GAATACCATAGCGAAAATCT+GGG | - | chr4.3:42221167-42221186 | MS.gene062572:CDS | 35.0% | |
| GGATATGGTATCAACTATCA+AGG | + | chr4.3:42221005-42221024 | None:intergenic | 35.0% | |
| GGATTGTATTGAAGAAGTTG+AGG | + | chr4.3:42220647-42220666 | None:intergenic | 35.0% | |
| TTCAAGAATGTACCTGTCAT+AGG | - | chr4.3:42221378-42221397 | MS.gene062572:CDS | 35.0% | |
| ! | AAGTGAAGCTACATGGATTT+TGG | - | chr4.3:42220531-42220550 | MS.gene062572:CDS | 35.0% |
| ! | GTACAGTCCTTTTACTTTGA+GGG | - | chr4.3:42220553-42220572 | MS.gene062572:CDS | 35.0% |
| ! | GTATTGACCCTTAAGCTAAA+AGG | - | chr4.3:42220578-42220597 | MS.gene062572:CDS | 35.0% |
| AACATTCTGGACATAGCTCT+TGG | - | chr4.3:42221258-42221277 | MS.gene062572:CDS | 40.0% | |
| AAGGATCAACAGGAAGCAAA+GGG | + | chr4.3:42220766-42220785 | None:intergenic | 40.0% | |
| GCTATCAGTTTCTCATGATC+AGG | + | chr4.3:42221414-42221433 | None:intergenic | 40.0% | |
| TAAGGATCAACAGGAAGCAA+AGG | + | chr4.3:42220767-42220786 | None:intergenic | 40.0% | |
| ! | ATGTTCCCAGATTTTCGCTA+TGG | + | chr4.3:42221175-42221194 | None:intergenic | 40.0% |
| ! | GGAAGATTTTCACCTATGAC+AGG | + | chr4.3:42221393-42221412 | None:intergenic | 40.0% |
| ! | GGTACAGTCCTTTTACTTTG+AGG | - | chr4.3:42220552-42220571 | MS.gene062572:CDS | 40.0% |
| AATTCCCTCACTTGCATGCA+TGG | - | chr4.3:42221346-42221365 | MS.gene062572:CDS | 45.0% | |
| AGGAGACACCGTTAACATTC+TGG | - | chr4.3:42221245-42221264 | MS.gene062572:CDS | 45.0% | |
| CAAACCATGCATGCAAGTGA+GGG | + | chr4.3:42221353-42221372 | None:intergenic | 45.0% | |
| GCAGATCAAGTGAAGCTACA+TGG | - | chr4.3:42220524-42220543 | MS.gene062572:CDS | 45.0% | |
| GCTCTCTCATAAGGATCAAC+AGG | + | chr4.3:42220776-42220795 | None:intergenic | 45.0% | |
| TCAAACCATGCATGCAAGTG+AGG | + | chr4.3:42221354-42221373 | None:intergenic | 45.0% | |
| TCCAGTGCACAAGAAAACTC+CGG | - | chr4.3:42220664-42220683 | MS.gene062572:CDS | 45.0% | |
| TTTCCACCATGAACCAACAC+CGG | + | chr4.3:42220686-42220705 | None:intergenic | 45.0% | |
| ! | ATTTTACCCAGAACCGAGCT+TGG | + | chr4.3:42220799-42220818 | None:intergenic | 45.0% |
| ! | TTTTACCCAGAACCGAGCTT+GGG | + | chr4.3:42220798-42220817 | None:intergenic | 45.0% |
| !! | AAAACTCCGGTGTTGGTTCA+TGG | - | chr4.3:42220677-42220696 | MS.gene062572:CDS | 45.0% |
| !! | AGTATTATAAATAATTTATT+GGG | - | chr4.3:42221081-42221100 | MS.gene062572:intron | 5.0% |
| !! | ATAAAAAAAAATAATATTGT+TGG | + | chr4.3:42221026-42221045 | None:intergenic | 5.0% |
| !! | TAGTATTATAAATAATTTAT+TGG | - | chr4.3:42221080-42221099 | MS.gene062572:intron | 5.0% |
| CTTATGAGAGAGCCCAAGCT+CGG | - | chr4.3:42220783-42220802 | MS.gene062572:CDS | 50.0% | |
| GAGGGAATTTCTCAGCTGCT+AGG | + | chr4.3:42221335-42221354 | None:intergenic | 50.0% | |
| GCACAAGAAAACTCCGGTGT+TGG | - | chr4.3:42220670-42220689 | MS.gene062572:CDS | 50.0% | |
| GTTGGTGCACTCGTTAGTAG+CGG | - | chr4.3:42221126-42221145 | MS.gene062572:intron | 50.0% | |
| ! | ACCGGAGTTTTCTTGTGCAC+TGG | + | chr4.3:42220668-42220687 | None:intergenic | 50.0% |
| !! | ACAGGAAGCAAAGGGTACTG+TGG | + | chr4.3:42220758-42220777 | None:intergenic | 50.0% |
| CGAGCTTGGGCTCTCTCATA+AGG | + | chr4.3:42220785-42220804 | None:intergenic | 55.0% | |
| CTAACGAGTGCACCAACTGC+AGG | + | chr4.3:42221123-42221142 | None:intergenic | 55.0% | |
| !! | ACTCCGGTGTTGGTTCATGG+TGG | - | chr4.3:42220680-42220699 | MS.gene062572:CDS | 55.0% |
| AGAGAGCCCAAGCTCGGTTC+TGG | - | chr4.3:42220789-42220808 | MS.gene062572:CDS | 60.0% | |
| GAGAGCCCAAGCTCGGTTCT+GGG | - | chr4.3:42220790-42220809 | MS.gene062572:CDS | 60.0% | |
| GGTGCACTCGTTAGTAGCGG+TGG | - | chr4.3:42221129-42221148 | MS.gene062572:intron | 60.0% | |
| GTAGCGGTGGCGATGAAGAG+CGG | - | chr4.3:42221142-42221161 | MS.gene062572:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 42220521 | 42221461 | 42220521 | ID=MS.gene062572 |
| chr4.3 | mRNA | 42220521 | 42221461 | 42220521 | ID=MS.gene062572.t1;Parent=MS.gene062572 |
| chr4.3 | exon | 42221150 | 42221461 | 42221150 | ID=MS.gene062572.t1.exon1;Parent=MS.gene062572.t1 |
| chr4.3 | CDS | 42221150 | 42221461 | 42221150 | ID=cds.MS.gene062572.t1;Parent=MS.gene062572.t1 |
| chr4.3 | exon | 42220521 | 42220865 | 42220521 | ID=MS.gene062572.t1.exon2;Parent=MS.gene062572.t1 |
| chr4.3 | CDS | 42220521 | 42220865 | 42220521 | ID=cds.MS.gene062572.t1;Parent=MS.gene062572.t1 |
| Gene Sequence |
| Protein sequence |
