AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene75261


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75261.t1 MTR_0097s0070 96.020 201 8 0 1 201 1 201 1.85e-142 395
MS.gene75261.t1 MTR_8g089300 73.196 194 51 1 1 194 1 193 2.77e-94 273
MS.gene75261.t1 MTR_4g118810 36.986 146 86 3 29 171 35 177 4.65e-22 89.4
MS.gene75261.t1 MTR_7g108130 31.609 174 112 3 17 189 18 185 7.52e-21 85.9
MS.gene75261.t1 MTR_0004s0210 32.927 164 94 2 29 192 10 157 1.03e-20 84.7
MS.gene75261.t1 MTR_2g020060 28.659 164 106 3 31 189 27 184 5.62e-15 70.5
MS.gene75261.t1 MTR_7g011090 27.957 186 121 3 6 188 10 185 1.07e-13 67.0
MS.gene75261.t1 MTR_4g081880 30.189 159 96 4 31 185 33 180 4.97e-12 62.4
MS.gene75261.t1 MTR_5g041900 30.323 155 101 1 31 185 32 179 1.73e-11 60.8
MS.gene75261.t1 MTR_1g058540 33.562 146 84 4 31 173 42 177 7.86e-11 59.3
MS.gene75261.t1 MTR_2g086740 29.268 123 71 2 71 185 99 213 8.21e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75261.t1 AT5G44550 58.721 172 71 0 23 194 14 185 1.85e-66 202
MS.gene75261.t1 AT4G20390 55.882 170 74 1 22 190 12 181 5.07e-54 171
MS.gene75261.t1 AT4G15620 29.570 186 124 4 5 189 10 189 2.22e-18 79.3
MS.gene75261.t1 AT1G03700 28.659 164 101 3 29 192 11 158 8.07e-18 77.4
MS.gene75261.t1 AT4G15630 28.022 182 125 3 9 189 13 189 1.22e-17 77.4
MS.gene75261.t1 AT4G15610 30.556 144 93 3 31 172 33 171 5.54e-16 73.2
MS.gene75261.t1 AT1G14160 29.032 155 103 1 31 185 55 202 6.80e-16 73.2
MS.gene75261.t1 AT5G15290 30.769 143 90 3 31 172 32 166 3.75e-15 70.9
MS.gene75261.t1 AT3G06390 30.827 133 84 3 32 161 42 169 8.76e-14 67.4
MS.gene75261.t1 AT4G03540 29.008 131 77 3 44 174 26 140 4.39e-13 64.7

Find 41 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTGGAGTTTGATTAAATT+TGG 0.215698 2.1:-43738139 None:intergenic
GCCCTTATTGCTTCTTTCAT+TGG 0.228754 2.1:+43739007 MS.gene75261:CDS
GCAGAAAGAGTAGCAGTTAT+TGG 0.256041 2.1:-43738117 None:intergenic
AAGTCCTTTGTAATCATATT+GGG 0.274380 2.1:-43738709 None:intergenic
ATGATTGCATTGGATATATT+AGG 0.293339 2.1:+43738684 MS.gene75261:CDS
CAGAAAGAGTAGCAGTTATT+GGG 0.306576 2.1:-43738116 None:intergenic
TACAAAATGGCTTCAAGAAA+TGG 0.318606 2.1:+43737922 None:intergenic
GAAGTCCTTTGTAATCATAT+TGG 0.328703 2.1:-43738710 None:intergenic
AGCCAATGAAAGAAGCAATA+AGG 0.340360 2.1:-43739009 None:intergenic
GGTGCCAATAACAAAACTTT+TGG 0.346894 2.1:-43738082 None:intergenic
TTTGAAAGCTATTGCAATAG+AGG 0.418883 2.1:+43738977 MS.gene75261:CDS
ATGTCAGCATTGGGAAGAAA+TGG 0.423404 2.1:+43738917 MS.gene75261:CDS
GCCAATGAAAGAAGCAATAA+GGG 0.431019 2.1:-43739008 None:intergenic
GGAGCATGGTTAATGCGATT+AGG 0.431376 2.1:-43739082 None:intergenic
TTGAAAACTTACACAAAAGC+TGG 0.437745 2.1:-43738156 None:intergenic
GCGATTAGGTTTGAGAAGCT+TGG 0.466239 2.1:-43739068 None:intergenic
ATGCAATCATCACCAAGTTA+TGG 0.467360 2.1:-43738671 None:intergenic
AGCAACATTCATGTCAGCAT+TGG 0.488005 2.1:+43738907 MS.gene75261:CDS
ACTATGGCTCTGGCATCAGC+TGG 0.494373 2.1:+43738875 MS.gene75261:CDS
GCAACATTCATGTCAGCATT+GGG 0.495269 2.1:+43738908 MS.gene75261:CDS
AAATGCTAGTCTCCATAACT+TGG 0.496794 2.1:+43738659 MS.gene75261:CDS
GGTGAAGTTAACTATGGCTC+TGG 0.500555 2.1:+43738865 MS.gene75261:intron
TGCAGCTGCAACACTTGTAA+TGG 0.510923 2.1:+43738044 MS.gene75261:CDS
TGGTAATTCACATGCAAGAT+GGG 0.512674 2.1:+43738937 MS.gene75261:CDS
ATGGTAATTCACATGCAAGA+TGG 0.522902 2.1:+43738936 MS.gene75261:CDS
ATGGAGACTAGCATTTCCAT+TGG 0.523367 2.1:-43738652 None:intergenic
AGGTTCTTTGTGATAGCCAA+TGG 0.526185 2.1:+43738636 MS.gene75261:intron
GCTCTGGCATCAGCTGGAGA+TGG 0.540036 2.1:+43738881 MS.gene75261:CDS
TGTTAATCATATCAAGGACT+AGG 0.549879 2.1:-43739103 None:intergenic
TAACTTGGTGATGATTGCAT+TGG 0.550469 2.1:+43738674 MS.gene75261:CDS
AGGTTTGAGAAGCTTGGCAA+TGG 0.555323 2.1:-43739062 None:intergenic
TACAAGTGTTGCAGCTGCAG+TGG 0.571483 2.1:-43738040 None:intergenic
CTAAAATTGCAATAAATGCA+AGG 0.578209 2.1:-43738734 None:intergenic
AGCTATTGCAATAGAGGTGG+TGG 0.598102 2.1:+43738983 MS.gene75261:CDS
CATATCAAGGACTAGGAGCA+TGG 0.606251 2.1:-43739096 None:intergenic
GAAAGCTATTGCAATAGAGG+TGG 0.619990 2.1:+43738980 MS.gene75261:CDS
AGTTATTGGGGTGTTACCAA+CGG 0.622547 2.1:-43738103 None:intergenic
AGTCCTTTGTAATCATATTG+GGG 0.628572 2.1:-43738708 None:intergenic
AGAAAGAGTAGCAGTTATTG+GGG 0.633112 2.1:-43738115 None:intergenic
GGACCCCAATATGATTACAA+AGG 0.645480 2.1:+43738705 MS.gene75261:CDS
GGTGAAGAGAAAGTTGAACG+TGG 0.756724 2.1:+43737943 MS.gene75261:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATACATTTATATTAACAT+GGG + chr2.1:43738606-43738625 MS.gene75261:intron 10.0%
!! TTTATACATTTATATTAACA+TGG + chr2.1:43738605-43738624 MS.gene75261:intron 10.0%
!!! AAGTTTTTTAATTTTTTCTT+AGG + chr2.1:43738796-43738815 MS.gene75261:intron 10.0%
!! TATACATTTATATTAACATG+GGG + chr2.1:43738607-43738626 MS.gene75261:intron 15.0%
!! AAAGAAAAACAAAGAATGAT+TGG + chr2.1:43737983-43738002 MS.gene75261:CDS 20.0%
!!! AAACCAAAAAAGTTTTATGT+AGG + chr2.1:43738375-43738394 MS.gene75261:intron 20.0%
!!! ATTTATTGCAATTTTAGACA+TGG + chr2.1:43738740-43738759 MS.gene75261:CDS 20.0%
! AAGTCCTTTGTAATCATATT+GGG - chr2.1:43738712-43738731 None:intergenic 25.0%
! AATTTCATCGCTTAATTCAA+AGG + chr2.1:43738502-43738521 MS.gene75261:intron 25.0%
! ATGATTGCATTGGATATATT+AGG + chr2.1:43738684-43738703 MS.gene75261:CDS 25.0%
! CTAAAATTGCAATAAATGCA+AGG - chr2.1:43738737-43738756 None:intergenic 25.0%
!! ATTGGATTCTTTTATCTCTT+CGG + chr2.1:43738001-43738020 MS.gene75261:CDS 25.0%
ACGATTGAGAAAATAGAGAA+AGG + chr2.1:43738458-43738477 MS.gene75261:intron 30.0%
AGCTGGAGTTTGATTAAATT+TGG - chr2.1:43738142-43738161 None:intergenic 30.0%
AGTCCTTTGTAATCATATTG+GGG - chr2.1:43738711-43738730 None:intergenic 30.0%
GAAGTCCTTTGTAATCATAT+TGG - chr2.1:43738713-43738732 None:intergenic 30.0%
TTGAAAACTTACACAAAAGC+TGG - chr2.1:43738159-43738178 None:intergenic 30.0%
TTTGAAAGCTATTGCAATAG+AGG + chr2.1:43738977-43738996 MS.gene75261:CDS 30.0%
! TTGGATTCTTTTATCTCTTC+GGG + chr2.1:43738002-43738021 MS.gene75261:CDS 30.0%
!! CCAAAAAAGTTTTATGTAGG+AGG + chr2.1:43738378-43738397 MS.gene75261:intron 30.0%
!!! CAGACCAAAAGTTTTGTTAT+TGG + chr2.1:43738078-43738097 MS.gene75261:CDS 30.0%
!!! CCTCCTACATAAAACTTTTT+TGG - chr2.1:43738381-43738400 None:intergenic 30.0%
!!! TTTTTGGGTGAAGTTAACTA+TGG + chr2.1:43738859-43738878 MS.gene75261:intron 30.0%
AGAAAGAGTAGCAGTTATTG+GGG - chr2.1:43738118-43738137 None:intergenic 35.0%
AGCCAATGAAAGAAGCAATA+AGG - chr2.1:43739012-43739031 None:intergenic 35.0%
ATACCATTGTACTACTCGTT+CGG - chr2.1:43738347-43738366 None:intergenic 35.0%
ATATTAACATGGGGTGATAC+AGG + chr2.1:43738616-43738635 MS.gene75261:intron 35.0%
ATGCAATCATCACCAAGTTA+TGG - chr2.1:43738674-43738693 None:intergenic 35.0%
ATGGTAATTCACATGCAAGA+TGG + chr2.1:43738936-43738955 MS.gene75261:CDS 35.0%
CAGAAAGAGTAGCAGTTATT+GGG - chr2.1:43738119-43738138 None:intergenic 35.0%
GCCAATGAAAGAAGCAATAA+GGG - chr2.1:43739011-43739030 None:intergenic 35.0%
TAGTAGAAGAGGTCGAATAT+CGG - chr2.1:43738405-43738424 None:intergenic 35.0%
TGGTAATTCACATGCAAGAT+GGG + chr2.1:43738937-43738956 MS.gene75261:CDS 35.0%
! AAATGCTAGTCTCCATAACT+TGG + chr2.1:43738659-43738678 MS.gene75261:CDS 35.0%
! CTCTATTTTCTCAATCGTAG+TGG - chr2.1:43738456-43738475 None:intergenic 35.0%
! GGTGCCAATAACAAAACTTT+TGG - chr2.1:43738085-43738104 None:intergenic 35.0%
!! ATCGTAGTGGTTAATTTGTC+AGG - chr2.1:43738443-43738462 None:intergenic 35.0%
!! TAACTTGGTGATGATTGCAT+TGG + chr2.1:43738674-43738693 MS.gene75261:CDS 35.0%
!! TCGTAGTGGTTAATTTGTCA+GGG - chr2.1:43738442-43738461 None:intergenic 35.0%
AGCAACATTCATGTCAGCAT+TGG + chr2.1:43738907-43738926 MS.gene75261:CDS 40.0%
AGGTTCTTTGTGATAGCCAA+TGG + chr2.1:43738636-43738655 MS.gene75261:intron 40.0%
ATGGAGACTAGCATTTCCAT+TGG - chr2.1:43738655-43738674 None:intergenic 40.0%
GAAAGCTATTGCAATAGAGG+TGG + chr2.1:43738980-43738999 MS.gene75261:CDS 40.0%
GAGGGAAATGTTAGTAGAAG+AGG - chr2.1:43738416-43738435 None:intergenic 40.0%
GCAACATTCATGTCAGCATT+GGG + chr2.1:43738908-43738927 MS.gene75261:CDS 40.0%
GCAGAAAGAGTAGCAGTTAT+TGG - chr2.1:43738120-43738139 None:intergenic 40.0%
GCCCTTATTGCTTCTTTCAT+TGG + chr2.1:43739007-43739026 MS.gene75261:CDS 40.0%
GGACCCCAATATGATTACAA+AGG + chr2.1:43738705-43738724 MS.gene75261:CDS 40.0%
TACCATTGTACTACTCGTTC+GGG - chr2.1:43738346-43738365 None:intergenic 40.0%
! AGTTATTGGGGTGTTACCAA+CGG - chr2.1:43738106-43738125 None:intergenic 40.0%
! ATGTCAGCATTGGGAAGAAA+TGG + chr2.1:43738917-43738936 MS.gene75261:CDS 40.0%
! GATTCTTTTATCTCTTCGGG+TGG + chr2.1:43738005-43738024 MS.gene75261:CDS 40.0%
!! GTTAATTTGTCAGGGAGAGA+GGG - chr2.1:43738434-43738453 None:intergenic 40.0%
!!! AGTTTTGTTATTGGCACCGT+TGG + chr2.1:43738087-43738106 MS.gene75261:CDS 40.0%
ACCATTGTACTACTCGTTCG+GGG - chr2.1:43738345-43738364 None:intergenic 45.0%
AGCTATTGCAATAGAGGTGG+TGG + chr2.1:43738983-43739002 MS.gene75261:CDS 45.0%
AGGTTTGAGAAGCTTGGCAA+TGG - chr2.1:43739065-43739084 None:intergenic 45.0%
GCGATTAGGTTTGAGAAGCT+TGG - chr2.1:43739071-43739090 None:intergenic 45.0%
GGAGCATGGTTAATGCGATT+AGG - chr2.1:43739085-43739104 None:intergenic 45.0%
GGTGAAGAGAAAGTTGAACG+TGG + chr2.1:43737943-43737962 MS.gene75261:CDS 45.0%
GGTGAAGTTAACTATGGCTC+TGG + chr2.1:43738865-43738884 MS.gene75261:intron 45.0%
TGCAGCTGCAACACTTGTAA+TGG + chr2.1:43738044-43738063 MS.gene75261:CDS 45.0%
!! GGTTAATTTGTCAGGGAGAG+AGG - chr2.1:43738435-43738454 None:intergenic 45.0%
!! ATCTATAATAAAATTAAAAT+AGG - chr2.1:43738584-43738603 None:intergenic 5.0%
!!! ATTGATAAATAATATTTTTT+TGG + chr2.1:43738843-43738862 MS.gene75261:intron 5.0%
!!! TTGATAAATAATATTTTTTT+GGG + chr2.1:43738844-43738863 MS.gene75261:intron 5.0%
TCCCCGAACGAGTAGTACAA+TGG + chr2.1:43738341-43738360 MS.gene75261:intron 50.0%
! TACAAGTGTTGCAGCTGCAG+TGG - chr2.1:43738043-43738062 None:intergenic 50.0%
!! ACTATGGCTCTGGCATCAGC+TGG + chr2.1:43738875-43738894 MS.gene75261:CDS 55.0%
GCTCTGGCATCAGCTGGAGA+TGG + chr2.1:43738881-43738900 MS.gene75261:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 43737928 43739114 43737928 ID=MS.gene75261
chr2.1 mRNA 43737928 43739114 43737928 ID=MS.gene75261.t1;Parent=MS.gene75261
chr2.1 exon 43737928 43738166 43737928 ID=MS.gene75261.t1.exon1;Parent=MS.gene75261.t1
chr2.1 CDS 43737928 43738166 43737928 ID=cds.MS.gene75261.t1;Parent=MS.gene75261.t1
chr2.1 exon 43738638 43738761 43738638 ID=MS.gene75261.t1.exon2;Parent=MS.gene75261.t1
chr2.1 CDS 43738638 43738761 43738638 ID=cds.MS.gene75261.t1;Parent=MS.gene75261.t1
chr2.1 exon 43738872 43739114 43738872 ID=MS.gene75261.t1.exon3;Parent=MS.gene75261.t1
chr2.1 CDS 43738872 43739114 43738872 ID=cds.MS.gene75261.t1;Parent=MS.gene75261.t1
Gene Sequence

>MS.gene75261

ATGGCTTCAAGAAATGGTGAAGAGAAAGTTGAACGTGGCTTCAATGATGTTCATGAAAGAAAAACAAAGAATGATTGGATTCTTTTATCTCTTCGGGTGGCTGCATTTTTTGCCACTGCAGCTGCAACACTTGTAATGGCACTCAACAAACAGACCAAAAGTTTTGTTATTGGCACCGTTGGTAACACCCCAATAACTGCTACTCTTTCTGCCAAATTTAATCAAACTCCAGCTTTTGTGTAAGTTTTCAATAATTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATACACAAATATCTATGATTCTACTTTTCTCTTTTTTATTTATGTTCGTTTGCAATGACTACTAGAAAAAAAATATAAGTAGTAACAATAGAGTCCCCGAACGAGTAGTACAATGGTATCGATTCATAAACCAAAAAAGTTTTATGTAGGAGGTCCGATATTCGACCTCTTCTACTAACATTTCCCTCTCTCCCTGACAAATTAACCACTACGATTGAGAAAATAGAGAAAGGAAATTGTGATGATCTATCTCTAATTTCATCGCTTAATTCAAAGGCGATGAATTTAAAGATATGTTTATTATTTTTTTATTTTTATTTTTGTGATGATATTCCTATTTTAATTTTATTATAGATTTTTATACATTTATATTAACATGGGGTGATACAGGTTCTTTGTGATAGCCAATGGAAATGCTAGTCTCCATAACTTGGTGATGATTGCATTGGATATATTAGGACCCCAATATGATTACAAAGGACTTCGCCTTGCATTTATTGCAATTTTAGACATGGTAATTTTTTTAATTAATTTTACATCAATTCTACAAGTTTTTTAATTTTTTCTTAGGATATCATTTATGTAAGAAAAAATGATTGATAAATAATATTTTTTTGGGTGAAGTTAACTATGGCTCTGGCATCAGCTGGAGATGGTGCAGCAACATTCATGTCAGCATTGGGAAGAAATGGTAATTCACATGCAAGATGGGATAAGATTTGTGACAAATTTGAAAGCTATTGCAATAGAGGTGGTGGTGCCCTTATTGCTTCTTTCATTGGCTTCATTCTTCTCTTGATTATCACTGTGATGTCCATTGCCAAGCTTCTCAAACCTAATCGCATTAACCATGCTCCTAGTCCTTGA

Protein sequence

>MS.gene75261.t1

MASRNGEEKVERGFNDVHERKTKNDWILLSLRVAAFFATAAATLVMALNKQTKSFVIGTVGNTPITATLSAKFNQTPAFVFFVIANGNASLHNLVMIALDILGPQYDYKGLRLAFIAILDMLTMALASAGDGAATFMSALGRNGNSHARWDKICDKFESYCNRGGGALIASFIGFILLLIITVMSIAKLLKPNRINHAPSP