AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene98021


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene98021.t1 MTR_0097s0070 95.025 201 10 0 1 201 1 201 8.23e-133 371
MS.gene98021.t1 MTR_8g089300 72.680 194 52 1 1 194 1 193 8.18e-94 272
MS.gene98021.t1 MTR_4g118810 36.986 146 86 3 29 171 35 177 1.20e-22 90.9
MS.gene98021.t1 MTR_7g108130 31.429 175 113 3 16 189 17 185 3.23e-21 87.0
MS.gene98021.t1 MTR_0004s0210 32.927 164 94 2 29 192 10 157 1.13e-20 84.7
MS.gene98021.t1 MTR_2g020060 28.659 164 106 3 31 189 27 184 2.23e-15 71.2
MS.gene98021.t1 MTR_7g011090 27.957 186 121 3 6 188 10 185 6.98e-14 67.4
MS.gene98021.t1 MTR_4g081880 30.189 159 96 4 31 185 33 180 3.30e-12 62.8
MS.gene98021.t1 MTR_5g041900 30.323 155 101 1 31 185 32 179 2.44e-11 60.5
MS.gene98021.t1 MTR_1g058540 33.562 146 84 4 31 173 42 177 5.58e-11 59.7
MS.gene98021.t1 MTR_2g086740 27.607 163 95 3 31 185 66 213 7.65e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene98021.t1 AT5G44550 58.140 172 72 0 23 194 14 185 2.25e-65 200
MS.gene98021.t1 AT4G20390 55.294 170 75 1 22 190 12 181 3.23e-53 169
MS.gene98021.t1 AT4G15620 29.570 186 124 4 5 189 10 189 8.45e-19 80.5
MS.gene98021.t1 AT4G15630 28.022 182 125 3 9 189 13 189 4.92e-18 78.6
MS.gene98021.t1 AT1G03700 28.049 164 102 3 29 192 11 158 2.15e-17 76.3
MS.gene98021.t1 AT4G15610 30.070 143 95 3 31 172 33 171 8.75e-16 72.8
MS.gene98021.t1 AT1G14160 28.387 155 104 1 31 185 55 202 1.46e-15 72.4
MS.gene98021.t1 AT5G15290 30.769 143 90 3 31 172 32 166 2.16e-15 71.2
MS.gene98021.t1 AT3G06390 30.827 133 84 3 32 161 42 169 3.97e-14 68.2
MS.gene98021.t1 AT4G03540 30.841 107 67 2 68 174 41 140 9.83e-13 63.9

Find 41 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTGGAGTTTGATTAAATT+TGG 0.215698 2.3:-43265559 None:intergenic
GCCCTTATTGCTTCTTTCAT+TGG 0.228754 2.3:+43266422 MS.gene98021:CDS
GCAGAAATAGTAGCAGTTAT+TGG 0.231666 2.3:-43265537 None:intergenic
CAGAAATAGTAGCAGTTATT+GGG 0.254239 2.3:-43265536 None:intergenic
AAGTCCTTTGTAATCATATT+GGG 0.274380 2.3:-43266123 None:intergenic
ATGATTGCATTGGATATATT+AGG 0.293339 2.3:+43266098 MS.gene98021:CDS
TACAAAATGGCTTCAAGAAA+TGG 0.318606 2.3:+43265342 None:intergenic
GAAGTCCTTTGTAATCATAT+TGG 0.328703 2.3:-43266124 None:intergenic
AGCCAATGAAAGAAGCAATA+AGG 0.340360 2.3:-43266424 None:intergenic
GGTGTCAATAACAAAACTTT+TGG 0.404127 2.3:-43265502 None:intergenic
TTTGAAAGCTATTGCAATAG+AGG 0.418883 2.3:+43266392 MS.gene98021:CDS
ATGTCAGCATTGGGAAGAAA+TGG 0.423404 2.3:+43266332 MS.gene98021:CDS
GCCAATGAAAGAAGCAATAA+GGG 0.431019 2.3:-43266423 None:intergenic
GGAGCATGGTTAATGCGATT+AGG 0.431376 2.3:-43266497 None:intergenic
TTGAAAACTTACACAAAAGC+TGG 0.466135 2.3:-43265576 None:intergenic
GCGATTAGGTTTGAGAAGCT+TGG 0.466239 2.3:-43266483 None:intergenic
ATGCAATCATCACCAAGTTA+TGG 0.467360 2.3:-43266085 None:intergenic
AGCAACATTCATGTCAGCAT+TGG 0.488005 2.3:+43266322 MS.gene98021:CDS
ACTATGGCTCTGGCATCAGC+TGG 0.494373 2.3:+43266290 MS.gene98021:CDS
GCAACATTCATGTCAGCATT+GGG 0.495269 2.3:+43266323 MS.gene98021:CDS
AAATGCTAGTCTCCATAACT+TGG 0.496794 2.3:+43266073 MS.gene98021:CDS
GGTGAAGTTAACTATGGCTC+TGG 0.500555 2.3:+43266280 MS.gene98021:intron
TGCAGCTGCAACACTTGTAA+TGG 0.510923 2.3:+43265464 MS.gene98021:CDS
TGGTAATTCACATGCAAGAT+GGG 0.512674 2.3:+43266352 MS.gene98021:CDS
ATGGTAATTCACATGCAAGA+TGG 0.522902 2.3:+43266351 MS.gene98021:CDS
ATGGAGACTAGCATTTCCAT+TGG 0.523367 2.3:-43266066 None:intergenic
AGGTTCTTTGTGATAGCCAA+TGG 0.526185 2.3:+43266050 MS.gene98021:intron
GCTCTGGCATCAGCTGGAGA+TGG 0.540036 2.3:+43266296 MS.gene98021:CDS
TGTTAATCATATCAAGGACT+AGG 0.549879 2.3:-43266518 None:intergenic
TAACTTGGTGATGATTGCAT+TGG 0.550469 2.3:+43266088 MS.gene98021:CDS
AGGTTTGAGAAGCTTGGCAA+TGG 0.555323 2.3:-43266477 None:intergenic
TACAAGTGTTGCAGCTGCAG+TGG 0.571483 2.3:-43265460 None:intergenic
CTAAAATTGCAATAAATGCA+AGG 0.578209 2.3:-43266148 None:intergenic
AGCTATTGCAATAGAGGTGG+TGG 0.598102 2.3:+43266398 MS.gene98021:CDS
CATATCAAGGACTAGGAGCA+TGG 0.606251 2.3:-43266511 None:intergenic
GAAAGCTATTGCAATAGAGG+TGG 0.619990 2.3:+43266395 MS.gene98021:CDS
AGTTATTGGGGTGTTACCAA+CGG 0.622547 2.3:-43265523 None:intergenic
AGTCCTTTGTAATCATATTG+GGG 0.628572 2.3:-43266122 None:intergenic
AGAAATAGTAGCAGTTATTG+GGG 0.633112 2.3:-43265535 None:intergenic
GGACCCCAATATGATTACAA+AGG 0.645480 2.3:+43266119 MS.gene98021:CDS
GGTGAAGAGAAAGTTGAACG+TGG 0.756724 2.3:+43265363 MS.gene98021:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTTTTTAATTTTTTTCTT+AGG + chr2.3:43266211-43266230 MS.gene98021:intron 10.0%
!! AATCAATACTACTTATATTT+CGG - chr2.3:43265772-43265791 None:intergenic 15.0%
!! AAAGAAAAACAAAGAATGAT+TGG + chr2.3:43265403-43265422 MS.gene98021:CDS 20.0%
!!! AAACCAAAAAAGTTTTATGT+AGG + chr2.3:43265795-43265814 MS.gene98021:intron 20.0%
!!! ATTTATTGCAATTTTAGACA+TGG + chr2.3:43266154-43266173 MS.gene98021:CDS 20.0%
! AACGAACATAAATTGAAAAG+AGG - chr2.3:43265677-43265696 None:intergenic 25.0%
! AAGTCCTTTGTAATCATATT+GGG - chr2.3:43266126-43266145 None:intergenic 25.0%
! ATGATTGCATTGGATATATT+AGG + chr2.3:43266098-43266117 MS.gene98021:CDS 25.0%
! CTAAAATTGCAATAAATGCA+AGG - chr2.3:43266151-43266170 None:intergenic 25.0%
! TTACATTAACACATCATTGT+GGG + chr2.3:43266020-43266039 MS.gene98021:intron 25.0%
! TTTACATTAACACATCATTG+TGG + chr2.3:43266019-43266038 MS.gene98021:intron 25.0%
!! ATTGGATTCTTTTATCTCTT+CGG + chr2.3:43265421-43265440 MS.gene98021:CDS 25.0%
!! CTCTATTTTCTCAATATTAG+TGG - chr2.3:43265876-43265895 None:intergenic 25.0%
!!! ATATTAGTGGTTAATTTGTC+AGG - chr2.3:43265863-43265882 None:intergenic 25.0%
!!! TATTAGTGGTTAATTTGTCA+GGG - chr2.3:43265862-43265881 None:intergenic 25.0%
AGAAATAGTAGCAGTTATTG+GGG - chr2.3:43265538-43265557 None:intergenic 30.0%
AGCTGGAGTTTGATTAAATT+TGG - chr2.3:43265562-43265581 None:intergenic 30.0%
AGTCCTTTGTAATCATATTG+GGG - chr2.3:43266125-43266144 None:intergenic 30.0%
CAGAAATAGTAGCAGTTATT+GGG - chr2.3:43265539-43265558 None:intergenic 30.0%
GAAGTCCTTTGTAATCATAT+TGG - chr2.3:43266127-43266146 None:intergenic 30.0%
TACATTAACACATCATTGTG+GGG + chr2.3:43266021-43266040 MS.gene98021:intron 30.0%
TTGAAAACTTACACAAAAGC+TGG - chr2.3:43265579-43265598 None:intergenic 30.0%
TTTGAAAGCTATTGCAATAG+AGG + chr2.3:43266392-43266411 MS.gene98021:CDS 30.0%
! GGTGTCAATAACAAAACTTT+TGG - chr2.3:43265505-43265524 None:intergenic 30.0%
! TTGGATTCTTTTATCTCTTC+GGG + chr2.3:43265422-43265441 MS.gene98021:CDS 30.0%
!! ATTAGTGGTTAATTTGTCAG+GGG - chr2.3:43265861-43265880 None:intergenic 30.0%
!! CCAAAAAAGTTTTATGTAGG+AGG + chr2.3:43265798-43265817 MS.gene98021:intron 30.0%
!!! CCTCCTACATAAAACTTTTT+TGG - chr2.3:43265801-43265820 None:intergenic 30.0%
!!! TTTTTGGGTGAAGTTAACTA+TGG + chr2.3:43266274-43266293 MS.gene98021:intron 30.0%
AGCCAATGAAAGAAGCAATA+AGG - chr2.3:43266427-43266446 None:intergenic 35.0%
ATGCAATCATCACCAAGTTA+TGG - chr2.3:43266088-43266107 None:intergenic 35.0%
ATGGTAATTCACATGCAAGA+TGG + chr2.3:43266351-43266370 MS.gene98021:CDS 35.0%
GCAGAAATAGTAGCAGTTAT+TGG - chr2.3:43265540-43265559 None:intergenic 35.0%
GCCAATGAAAGAAGCAATAA+GGG - chr2.3:43266426-43266445 None:intergenic 35.0%
TAGTAGAAGAGGTCGAATAT+CGG - chr2.3:43265825-43265844 None:intergenic 35.0%
TGGTAATTCACATGCAAGAT+GGG + chr2.3:43266352-43266371 MS.gene98021:CDS 35.0%
! AAATGCTAGTCTCCATAACT+TGG + chr2.3:43266073-43266092 MS.gene98021:CDS 35.0%
!! TAACTTGGTGATGATTGCAT+TGG + chr2.3:43266088-43266107 MS.gene98021:CDS 35.0%
!! TTAGTGGTTAATTTGTCAGG+GGG - chr2.3:43265860-43265879 None:intergenic 35.0%
!!! AGTTTTGTTATTGACACCGT+TGG + chr2.3:43265507-43265526 MS.gene98021:CDS 35.0%
AGCAACATTCATGTCAGCAT+TGG + chr2.3:43266322-43266341 MS.gene98021:CDS 40.0%
AGGTTCTTTGTGATAGCCAA+TGG + chr2.3:43266050-43266069 MS.gene98021:intron 40.0%
ATGGAGACTAGCATTTCCAT+TGG - chr2.3:43266069-43266088 None:intergenic 40.0%
GAAAGCTATTGCAATAGAGG+TGG + chr2.3:43266395-43266414 MS.gene98021:CDS 40.0%
GAGGGAAATGTTAGTAGAAG+AGG - chr2.3:43265836-43265855 None:intergenic 40.0%
GCAACATTCATGTCAGCATT+GGG + chr2.3:43266323-43266342 MS.gene98021:CDS 40.0%
GCCCTTATTGCTTCTTTCAT+TGG + chr2.3:43266422-43266441 MS.gene98021:CDS 40.0%
GGACCCCAATATGATTACAA+AGG + chr2.3:43266119-43266138 MS.gene98021:CDS 40.0%
! AGTTATTGGGGTGTTACCAA+CGG - chr2.3:43265526-43265545 None:intergenic 40.0%
! ATGTCAGCATTGGGAAGAAA+TGG + chr2.3:43266332-43266351 MS.gene98021:CDS 40.0%
! GATTCTTTTATCTCTTCGGG+TGG + chr2.3:43265425-43265444 MS.gene98021:CDS 40.0%
ACATCATTGTGGGGTGATAC+AGG + chr2.3:43266030-43266049 MS.gene98021:intron 45.0%
AGCTATTGCAATAGAGGTGG+TGG + chr2.3:43266398-43266417 MS.gene98021:CDS 45.0%
AGGTTTGAGAAGCTTGGCAA+TGG - chr2.3:43266480-43266499 None:intergenic 45.0%
GCGATTAGGTTTGAGAAGCT+TGG - chr2.3:43266486-43266505 None:intergenic 45.0%
GGAGCATGGTTAATGCGATT+AGG - chr2.3:43266500-43266519 None:intergenic 45.0%
GGTGAAGAGAAAGTTGAACG+TGG + chr2.3:43265363-43265382 MS.gene98021:CDS 45.0%
GGTGAAGTTAACTATGGCTC+TGG + chr2.3:43266280-43266299 MS.gene98021:intron 45.0%
TGCAGCTGCAACACTTGTAA+TGG + chr2.3:43265464-43265483 MS.gene98021:CDS 45.0%
!! GTTAATTTGTCAGGGGGAGA+GGG - chr2.3:43265854-43265873 None:intergenic 45.0%
!!! ATTGATAAATAATATTTTTT+TGG + chr2.3:43266258-43266277 MS.gene98021:intron 5.0%
!!! TTGATAAATAATATTTTTTT+GGG + chr2.3:43266259-43266278 MS.gene98021:intron 5.0%
! TACAAGTGTTGCAGCTGCAG+TGG - chr2.3:43265463-43265482 None:intergenic 50.0%
!! GGTTAATTTGTCAGGGGGAG+AGG - chr2.3:43265855-43265874 None:intergenic 50.0%
!! ACTATGGCTCTGGCATCAGC+TGG + chr2.3:43266290-43266309 MS.gene98021:CDS 55.0%
AGAGTCCCCGCACGAGTAGT+AGG + chr2.3:43265742-43265761 MS.gene98021:intron 60.0%
CGGACCTCCTACTACTCGTG+CGG - chr2.3:43265752-43265771 None:intergenic 60.0%
GACCTCCTACTACTCGTGCG+GGG - chr2.3:43265750-43265769 None:intergenic 60.0%
GCTCTGGCATCAGCTGGAGA+TGG + chr2.3:43266296-43266315 MS.gene98021:CDS 60.0%
GGACCTCCTACTACTCGTGC+GGG - chr2.3:43265751-43265770 None:intergenic 60.0%
GTCCCCGCACGAGTAGTAGG+AGG + chr2.3:43265745-43265764 MS.gene98021:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 43265348 43266529 43265348 ID=MS.gene98021
chr2.3 mRNA 43265348 43266529 43265348 ID=MS.gene98021.t1;Parent=MS.gene98021
chr2.3 exon 43265348 43265586 43265348 ID=MS.gene98021.t1.exon1;Parent=MS.gene98021.t1
chr2.3 CDS 43265348 43265586 43265348 ID=cds.MS.gene98021.t1;Parent=MS.gene98021.t1
chr2.3 exon 43266052 43266175 43266052 ID=MS.gene98021.t1.exon2;Parent=MS.gene98021.t1
chr2.3 CDS 43266052 43266175 43266052 ID=cds.MS.gene98021.t1;Parent=MS.gene98021.t1
chr2.3 exon 43266287 43266529 43266287 ID=MS.gene98021.t1.exon3;Parent=MS.gene98021.t1
chr2.3 CDS 43266287 43266529 43266287 ID=cds.MS.gene98021.t1;Parent=MS.gene98021.t1
Gene Sequence

>MS.gene98021

ATGGCTTCAAGAAATGGTGAAGAGAAAGTTGAACGTGGCTTCAATGATGTTCATGAAAGAAAAACAAAGAATGATTGGATTCTTTTATCTCTTCGGGTGGCTGCATTTTTTGCCACTGCAGCTGCAACACTTGTAATGGCACTCAACAAACAGACCAAAAGTTTTGTTATTGACACCGTTGGTAACACCCCAATAACTGCTACTATTTCTGCCAAATTTAATCAAACTCCAGCTTTTGTGTAAGTTTTCAATAGTTATTATATATATATATATATATATATATATACACACACACACACACACACAAATATCTATGATTCTACTTTCCTCTTTTCAATTTATGTTCGTTTGCAATGTGACTACTAGAAAAAAAAATATAGTAGTAACAATAAATAGAGTCCCCGCACGAGTAGTAGGAGGTCCGAAATATAAGTAGTATTGATTCAGAAACCAAAAAAGTTTTATGTAGGAGGTCCGATATTCGACCTCTTCTACTAACATTTCCCTCTCCCCCTGACAAATTAACCACTAATATTGAGAAAATAGAGAAAGAAAATTGTTACGATATGTCTCTAATCTCATTGCTTAATTCAAAGACGATGAATTTAGAGACATGTTTAATTTTTTTGTGATGATATAGCTATTTTTATTTTATTATAGATTTTTATACATTTACATTAACACATCATTGTGGGGTGATACAGGTTCTTTGTGATAGCCAATGGAAATGCTAGTCTCCATAACTTGGTGATGATTGCATTGGATATATTAGGACCCCAATATGATTACAAAGGACTTCGCCTTGCATTTATTGCAATTTTAGACATGGTAATTTTTTTAATTAATTTTACATCAATTCTACAAGTTTTTTAATTTTTTTCTTAGGATATCATTTATGTAAGAAAAAATGATTGATAAATAATATTTTTTTGGGTGAAGTTAACTATGGCTCTGGCATCAGCTGGAGATGGTGCAGCAACATTCATGTCAGCATTGGGAAGAAATGGTAATTCACATGCAAGATGGGATAAGATTTGTGACAAATTTGAAAGCTATTGCAATAGAGGTGGTGGTGCCCTTATTGCTTCTTTCATTGGCTTCATTCTTCTCTTGATTATCACTGTGATGTCCATTGCCAAGCTTCTCAAACCTAATCGCATTAACCATGCTCCTAGTCCTTGA

Protein sequence

>MS.gene98021.t1

MASRNGEEKVERGFNDVHERKTKNDWILLSLRVAAFFATAAATLVMALNKQTKSFVIDTVGNTPITATISAKFNQTPAFVFFVIANGNASLHNLVMIALDILGPQYDYKGLRLAFIAILDMLTMALASAGDGAATFMSALGRNGNSHARWDKICDKFESYCNRGGGALIASFIGFILLLIITVMSIAKLLKPNRINHAPSP