Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061718.t1 | XP_013441833.1 | 98.2 | 218 | 4 | 0 | 1 | 218 | 117 | 334 | 1.50E-114 | 422.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061718.t1 | P81392 | 38.0 | 192 | 78 | 9 | 22 | 197 | 141 | 307 | 2.3e-10 | 67.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061718.t1 | A0A072TFY2 | 98.2 | 218 | 4 | 0 | 1 | 218 | 117 | 334 | 1.1e-114 | 422.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049356 | MS.gene061718 | 0.803765 | 2.98E-49 | -1.69E-46 |
| MS.gene049369 | MS.gene061718 | 0.813125 | 3.00E-51 | -1.69E-46 |
| MS.gene049396 | MS.gene061718 | 0.855983 | 4.37E-62 | -1.69E-46 |
| MS.gene050380 | MS.gene061718 | -0.802515 | 5.41E-49 | -1.69E-46 |
| MS.gene050499 | MS.gene061718 | 0.82081 | 5.63E-53 | -1.69E-46 |
| MS.gene050502 | MS.gene061718 | 0.850582 | 1.55E-60 | -1.69E-46 |
| MS.gene050686 | MS.gene061718 | 0.807189 | 5.71E-50 | -1.69E-46 |
| MS.gene05105 | MS.gene061718 | 0.817042 | 4.05E-52 | -1.69E-46 |
| MS.gene051509 | MS.gene061718 | 0.807781 | 4.27E-50 | -1.69E-46 |
| MS.gene052346 | MS.gene061718 | 0.809317 | 2.01E-50 | -1.69E-46 |
| MS.gene052414 | MS.gene061718 | 0.829816 | 4.17E-55 | -1.69E-46 |
| MS.gene053449 | MS.gene061718 | 0.835685 | 1.45E-56 | -1.69E-46 |
| MS.gene053505 | MS.gene061718 | 0.823164 | 1.60E-53 | -1.69E-46 |
| MS.gene053574 | MS.gene061718 | 0.818891 | 1.55E-52 | -1.69E-46 |
| MS.gene053813 | MS.gene061718 | 0.831505 | 1.61E-55 | -1.69E-46 |
| MS.gene054486 | MS.gene061718 | 0.805775 | 1.13E-49 | -1.69E-46 |
| MS.gene05505 | MS.gene061718 | 0.812438 | 4.24E-51 | -1.69E-46 |
| MS.gene055909 | MS.gene061718 | 0.861351 | 1.09E-63 | -1.69E-46 |
| MS.gene055911 | MS.gene061718 | 0.866621 | 2.49E-65 | -1.69E-46 |
| MS.gene056166 | MS.gene061718 | 0.814482 | 1.51E-51 | -1.69E-46 |
| MS.gene056422 | MS.gene061718 | 0.866724 | 2.31E-65 | -1.69E-46 |
| MS.gene056448 | MS.gene061718 | 0.823388 | 1.42E-53 | -1.69E-46 |
| MS.gene056511 | MS.gene061718 | 0.841974 | 3.43E-58 | -1.69E-46 |
| MS.gene056942 | MS.gene061718 | 0.800146 | 1.65E-48 | -1.69E-46 |
| MS.gene057086 | MS.gene061718 | 0.813154 | 2.95E-51 | -1.69E-46 |
| MS.gene057734 | MS.gene061718 | 0.801971 | 7.00E-49 | -1.69E-46 |
| MS.gene057749 | MS.gene061718 | 0.803156 | 3.99E-49 | -1.69E-46 |
| MS.gene057823 | MS.gene061718 | 0.817037 | 4.06E-52 | -1.69E-46 |
| MS.gene058075 | MS.gene061718 | 0.814996 | 1.16E-51 | -1.69E-46 |
| MS.gene058376 | MS.gene061718 | 0.817883 | 2.62E-52 | -1.69E-46 |
| MS.gene058928 | MS.gene061718 | 0.81983 | 9.45E-53 | -1.69E-46 |
| MS.gene058995 | MS.gene061718 | 0.847103 | 1.43E-59 | -1.69E-46 |
| MS.gene05913 | MS.gene061718 | 0.810587 | 1.07E-50 | -1.69E-46 |
| MS.gene059366 | MS.gene061718 | 0.821155 | 4.69E-53 | -1.69E-46 |
| MS.gene059367 | MS.gene061718 | 0.826076 | 3.31E-54 | -1.69E-46 |
| MS.gene059368 | MS.gene061718 | 0.866443 | 2.84E-65 | -1.69E-46 |
| MS.gene059517 | MS.gene061718 | 0.808941 | 2.42E-50 | -1.69E-46 |
| MS.gene059674 | MS.gene061718 | 0.81803 | 2.42E-52 | -1.69E-46 |
| MS.gene059807 | MS.gene061718 | 0.862404 | 5.19E-64 | -1.69E-46 |
| MS.gene059933 | MS.gene061718 | 0.833368 | 5.55E-56 | -1.69E-46 |
| MS.gene060401 | MS.gene061718 | 0.814826 | 1.26E-51 | -1.69E-46 |
| MS.gene060482 | MS.gene061718 | 0.826776 | 2.25E-54 | -1.69E-46 |
| MS.gene060804 | MS.gene061718 | 0.802064 | 6.70E-49 | -1.69E-46 |
| MS.gene060835 | MS.gene061718 | 0.803864 | 2.84E-49 | -1.69E-46 |
| MS.gene060837 | MS.gene061718 | 0.835398 | 1.72E-56 | -1.69E-46 |
| MS.gene06097 | MS.gene061718 | 0.818746 | 1.67E-52 | -1.69E-46 |
| MS.gene061041 | MS.gene061718 | 0.819073 | 1.41E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061718.t1 | MTR_0140s0030 | 98.165 | 218 | 4 | 0 | 1 | 218 | 117 | 334 | 6.55e-158 | 440 |
| MS.gene061718.t1 | MTR_0489s0020 | 98.165 | 218 | 4 | 0 | 1 | 218 | 117 | 334 | 6.55e-158 | 440 |
| MS.gene061718.t1 | MTR_3g039990 | 37.778 | 225 | 96 | 9 | 4 | 218 | 121 | 311 | 2.80e-30 | 114 |
| MS.gene061718.t1 | MTR_6g012180 | 36.041 | 197 | 107 | 7 | 11 | 205 | 130 | 309 | 6.20e-21 | 89.4 |
| MS.gene061718.t1 | MTR_7g010210 | 31.718 | 227 | 107 | 10 | 1 | 206 | 116 | 315 | 2.96e-16 | 76.3 |
| MS.gene061718.t1 | MTR_7g010210 | 40.678 | 118 | 43 | 4 | 1 | 105 | 116 | 219 | 8.05e-14 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061718.t1 | AT1G74650 | 33.333 | 198 | 99 | 7 | 22 | 211 | 74 | 246 | 3.41e-17 | 77.8 |
| MS.gene061718.t1 | AT1G74650 | 33.333 | 198 | 99 | 7 | 22 | 211 | 79 | 251 | 6.25e-17 | 77.4 |
| MS.gene061718.t1 | AT1G74650 | 33.333 | 198 | 99 | 7 | 22 | 211 | 155 | 327 | 3.63e-16 | 76.3 |
| MS.gene061718.t1 | AT3G47600 | 35.176 | 199 | 97 | 10 | 23 | 212 | 147 | 322 | 1.84e-15 | 74.3 |
| MS.gene061718.t1 | AT3G47600 | 35.176 | 199 | 97 | 10 | 23 | 212 | 153 | 328 | 2.41e-15 | 73.9 |
| MS.gene061718.t1 | AT3G28910 | 30.366 | 191 | 87 | 5 | 23 | 197 | 73 | 233 | 2.46e-14 | 70.1 |
| MS.gene061718.t1 | AT3G28910 | 30.366 | 191 | 87 | 5 | 23 | 197 | 149 | 309 | 8.80e-14 | 69.3 |
| MS.gene061718.t1 | AT5G62470 | 33.043 | 230 | 98 | 10 | 22 | 217 | 145 | 352 | 5.70e-13 | 67.4 |
| MS.gene061718.t1 | AT5G62470 | 33.043 | 230 | 98 | 10 | 22 | 217 | 144 | 351 | 6.43e-13 | 67.0 |
Find 44 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGATTATGCATCTCGATTT+AGG | 0.240478 | 8.1:-16727581 | MS.gene061718:CDS |
| GATTAATATTAAAGCAAATA+TGG | 0.292292 | 8.1:-16727487 | MS.gene061718:CDS |
| TGAAAACATTTCTAGATTGA+TGG | 0.297412 | 8.1:-16727739 | MS.gene061718:CDS |
| CTTTCCCATTGACCTTTATT+CGG | 0.331227 | 8.1:+16727939 | None:intergenic |
| AGCCTCAGATAAGGCTTGTT+TGG | 0.361155 | 8.1:+16727890 | None:intergenic |
| TCGAGATGCATAATCAGATT+TGG | 0.373847 | 8.1:+16727587 | None:intergenic |
| CGTTCGCAGCTGCCGAATAA+AGG | 0.376269 | 8.1:-16727951 | MS.gene061718:CDS |
| TTTGTTTCAGCTGATGAGTT+AGG | 0.376364 | 8.1:+16727699 | None:intergenic |
| ACATGTTTATAAGCTCTTCA+TGG | 0.378049 | 8.1:+16727397 | None:intergenic |
| GCTTATAAACATGTCATTAG+AGG | 0.404105 | 8.1:-16727388 | MS.gene061718:CDS |
| AATCAGATTTGGTTGAGTTA+AGG | 0.409124 | 8.1:+16727598 | None:intergenic |
| GATGAGGGTTTCATCTGATC+AGG | 0.419961 | 8.1:+16727828 | None:intergenic |
| TAATATTAATCCTTTCCTCT+TGG | 0.421047 | 8.1:+16727499 | None:intergenic |
| TATTAATCCTTTCCTCTTGG+TGG | 0.453369 | 8.1:+16727502 | None:intergenic |
| ATCGATATTCAGCAACATGC+AGG | 0.473325 | 8.1:-16727670 | MS.gene061718:CDS |
| TTTCTAGATTGATGGAAACT+TGG | 0.473758 | 8.1:-16727731 | MS.gene061718:CDS |
| GGCAAAGTACCAAGGGTTGT+AGG | 0.474388 | 8.1:+16727849 | None:intergenic |
| AGCTGCCGAATAAAGGTCAA+TGG | 0.474923 | 8.1:-16727944 | MS.gene061718:CDS |
| GGAGATGGAATACTCAGGTT+TGG | 0.477996 | 8.1:+16727777 | None:intergenic |
| GGGTTGTAGGGTTATGTTGA+AGG | 0.484469 | 8.1:+16727862 | None:intergenic |
| GCTACAAACAGATATTCATA+TGG | 0.516194 | 8.1:-16727913 | MS.gene061718:CDS |
| CTTCAACTTGAACACAAAAG+AGG | 0.519329 | 8.1:-16727535 | MS.gene061718:CDS |
| CAACATAACCCTACAACCCT+TGG | 0.522290 | 8.1:-16727858 | MS.gene061718:CDS |
| GCAAAGTACCAAGGGTTGTA+GGG | 0.526670 | 8.1:+16727850 | None:intergenic |
| GCTGCCGAATAAAGGTCAAT+GGG | 0.539519 | 8.1:-16727943 | MS.gene061718:CDS |
| ATAAAGGTCAATGGGAAAGA+AGG | 0.547179 | 8.1:-16727935 | MS.gene061718:CDS |
| TCTTCTTCCACCAAGAGGAA+AGG | 0.547540 | 8.1:-16727509 | MS.gene061718:CDS |
| TAGCTTGATGCATATGGAGA+TGG | 0.555943 | 8.1:+16727762 | None:intergenic |
| GATCAAAGTAGTAACGATGA+AGG | 0.558829 | 8.1:-16727990 | MS.gene061718:CDS |
| CGATTTAGGTCAACTCATGA+AGG | 0.562437 | 8.1:-16727567 | MS.gene061718:CDS |
| GATGGAATACTCAGGTTTGG+TGG | 0.569712 | 8.1:+16727780 | None:intergenic |
| TTCAACTTGAACACAAAAGA+GGG | 0.572702 | 8.1:-16727534 | MS.gene061718:CDS |
| GGTTGTAGGGTTATGTTGAA+GGG | 0.574998 | 8.1:+16727863 | None:intergenic |
| AAGGGACAAAGCCTCAGATA+AGG | 0.576279 | 8.1:+16727881 | None:intergenic |
| CTTATAAACATGTCATTAGA+GGG | 0.583066 | 8.1:-16727387 | MS.gene061718:CDS |
| CAGGGATCAAGTTGTAGTGA+AGG | 0.587215 | 8.1:-16727651 | MS.gene061718:CDS |
| TCGATATTCAGCAACATGCA+GGG | 0.617549 | 8.1:-16727669 | MS.gene061718:CDS |
| CTGATCAGGCAAAGTACCAA+GGG | 0.619842 | 8.1:+16727842 | None:intergenic |
| TCTGATCAGGCAAAGTACCA+AGG | 0.632255 | 8.1:+16727841 | None:intergenic |
| AGCACAACACAAGATCATCA+TGG | 0.642061 | 8.1:-16727621 | MS.gene061718:CDS |
| CATATGGAGATGGAATACTC+AGG | 0.654786 | 8.1:+16727772 | None:intergenic |
| GGGTCTCTTCTTCCACCAAG+AGG | 0.658408 | 8.1:-16727514 | MS.gene061718:CDS |
| GGCCAAACAAGCCTTATCTG+AGG | 0.663946 | 8.1:-16727892 | MS.gene061718:CDS |
| CATGTTGCTGAATATCGATG+AGG | 0.741627 | 8.1:+16727674 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATTAATATTAAAGCAAATA+TGG | - | chr8.1:16727877-16727896 | MS.gene061718:CDS | 15.0% |
| ! | CTTATAAACATGTCATTAGA+GGG | - | chr8.1:16727977-16727996 | MS.gene061718:CDS | 25.0% |
| ! | TAATATTAATCCTTTCCTCT+TGG | + | chr8.1:16727868-16727887 | None:intergenic | 25.0% |
| !! | TGAAAACATTTCTAGATTGA+TGG | - | chr8.1:16727625-16727644 | MS.gene061718:CDS | 25.0% |
| !!! | AAAAATGGCTTTTTGAAGAT+GGG | - | chr8.1:16727927-16727946 | MS.gene061718:CDS | 25.0% |
| ACATGTTTATAAGCTCTTCA+TGG | + | chr8.1:16727970-16727989 | None:intergenic | 30.0% | |
| AGAATGAGAAAAACTTGATG+AGG | + | chr8.1:16727555-16727574 | None:intergenic | 30.0% | |
| CTCTTACTTTGCTTGAAAAA+TGG | - | chr8.1:16727912-16727931 | MS.gene061718:CDS | 30.0% | |
| GAATGAGAAAAACTTGATGA+GGG | + | chr8.1:16727554-16727573 | None:intergenic | 30.0% | |
| GCTACAAACAGATATTCATA+TGG | - | chr8.1:16727451-16727470 | MS.gene061718:CDS | 30.0% | |
| GCTTATAAACATGTCATTAG+AGG | - | chr8.1:16727976-16727995 | MS.gene061718:CDS | 30.0% | |
| TTCAACTTGAACACAAAAGA+GGG | - | chr8.1:16727830-16727849 | MS.gene061718:CDS | 30.0% | |
| TTTCTAGATTGATGGAAACT+TGG | - | chr8.1:16727633-16727652 | MS.gene061718:CDS | 30.0% | |
| ! | AATCAGATTTGGTTGAGTTA+AGG | + | chr8.1:16727769-16727788 | None:intergenic | 30.0% |
| ! | TTTTCATAGCTTGATGCATA+TGG | + | chr8.1:16727611-16727630 | None:intergenic | 30.0% |
| !! | AAAATGGCTTTTTGAAGATG+GGG | - | chr8.1:16727928-16727947 | MS.gene061718:CDS | 30.0% |
| !! | GAAAAATGGCTTTTTGAAGA+TGG | - | chr8.1:16727926-16727945 | MS.gene061718:CDS | 30.0% |
| !!! | CATTTTTCAAGCAAAGTAAG+AGG | + | chr8.1:16727914-16727933 | None:intergenic | 30.0% |
| ATAAAGGTCAATGGGAAAGA+AGG | - | chr8.1:16727429-16727448 | MS.gene061718:CDS | 35.0% | |
| CTGATTATGCATCTCGATTT+AGG | - | chr8.1:16727783-16727802 | MS.gene061718:CDS | 35.0% | |
| CTTCAACTTGAACACAAAAG+AGG | - | chr8.1:16727829-16727848 | MS.gene061718:CDS | 35.0% | |
| CTTTCCCATTGACCTTTATT+CGG | + | chr8.1:16727428-16727447 | None:intergenic | 35.0% | |
| GATCAAAGTAGTAACGATGA+AGG | - | chr8.1:16727374-16727393 | MS.gene061718:CDS | 35.0% | |
| TATTAATCCTTTCCTCTTGG+TGG | + | chr8.1:16727865-16727884 | None:intergenic | 35.0% | |
| TCGAGATGCATAATCAGATT+TGG | + | chr8.1:16727780-16727799 | None:intergenic | 35.0% | |
| TTTGTTTCAGCTGATGAGTT+AGG | + | chr8.1:16727668-16727687 | None:intergenic | 35.0% | |
| !! | AAATGGCTTTTTGAAGATGG+GGG | - | chr8.1:16727929-16727948 | MS.gene061718:CDS | 35.0% |
| AGCACAACACAAGATCATCA+TGG | - | chr8.1:16727743-16727762 | MS.gene061718:CDS | 40.0% | |
| ATCGATATTCAGCAACATGC+AGG | - | chr8.1:16727694-16727713 | MS.gene061718:CDS | 40.0% | |
| CATATGGAGATGGAATACTC+AGG | + | chr8.1:16727595-16727614 | None:intergenic | 40.0% | |
| CATGTTGCTGAATATCGATG+AGG | + | chr8.1:16727693-16727712 | None:intergenic | 40.0% | |
| CGATTTAGGTCAACTCATGA+AGG | - | chr8.1:16727797-16727816 | MS.gene061718:CDS | 40.0% | |
| GGTTGTAGGGTTATGTTGAA+GGG | + | chr8.1:16727504-16727523 | None:intergenic | 40.0% | |
| TAGCTTGATGCATATGGAGA+TGG | + | chr8.1:16727605-16727624 | None:intergenic | 40.0% | |
| TCGATATTCAGCAACATGCA+GGG | - | chr8.1:16727695-16727714 | MS.gene061718:CDS | 40.0% | |
| AAGGGACAAAGCCTCAGATA+AGG | + | chr8.1:16727486-16727505 | None:intergenic | 45.0% | |
| AGCTGCCGAATAAAGGTCAA+TGG | - | chr8.1:16727420-16727439 | MS.gene061718:CDS | 45.0% | |
| CAACATAACCCTACAACCCT+TGG | - | chr8.1:16727506-16727525 | MS.gene061718:CDS | 45.0% | |
| GATGAGGGTTTCATCTGATC+AGG | + | chr8.1:16727539-16727558 | None:intergenic | 45.0% | |
| GATGGAATACTCAGGTTTGG+TGG | + | chr8.1:16727587-16727606 | None:intergenic | 45.0% | |
| GCTGCCGAATAAAGGTCAAT+GGG | - | chr8.1:16727421-16727440 | MS.gene061718:CDS | 45.0% | |
| GGAGATGGAATACTCAGGTT+TGG | + | chr8.1:16727590-16727609 | None:intergenic | 45.0% | |
| GGGTTGTAGGGTTATGTTGA+AGG | + | chr8.1:16727505-16727524 | None:intergenic | 45.0% | |
| TCTTCTTCCACCAAGAGGAA+AGG | - | chr8.1:16727855-16727874 | MS.gene061718:CDS | 45.0% | |
| ! | AGCCTCAGATAAGGCTTGTT+TGG | + | chr8.1:16727477-16727496 | None:intergenic | 45.0% |
| ! | CAGGGATCAAGTTGTAGTGA+AGG | - | chr8.1:16727713-16727732 | MS.gene061718:CDS | 45.0% |
| ! | CTGATCAGGCAAAGTACCAA+GGG | + | chr8.1:16727525-16727544 | None:intergenic | 45.0% |
| ! | GCAAAGTACCAAGGGTTGTA+GGG | + | chr8.1:16727517-16727536 | None:intergenic | 45.0% |
| ! | TCTGATCAGGCAAAGTACCA+AGG | + | chr8.1:16727526-16727545 | None:intergenic | 45.0% |
| ! | GGCAAAGTACCAAGGGTTGT+AGG | + | chr8.1:16727518-16727537 | None:intergenic | 50.0% |
| ! | GGCCAAACAAGCCTTATCTG+AGG | - | chr8.1:16727472-16727491 | MS.gene061718:CDS | 50.0% |
| CGTTCGCAGCTGCCGAATAA+AGG | - | chr8.1:16727413-16727432 | MS.gene061718:CDS | 55.0% | |
| GGGTCTCTTCTTCCACCAAG+AGG | - | chr8.1:16727850-16727869 | MS.gene061718:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 16727365 | 16728021 | 16727365 | ID=MS.gene061718 |
| chr8.1 | mRNA | 16727365 | 16728021 | 16727365 | ID=MS.gene061718.t1;Parent=MS.gene061718 |
| chr8.1 | exon | 16727365 | 16728021 | 16727365 | ID=MS.gene061718.t1.exon1;Parent=MS.gene061718.t1 |
| chr8.1 | CDS | 16727365 | 16728021 | 16727365 | ID=cds.MS.gene061718.t1;Parent=MS.gene061718.t1 |
| Gene Sequence |
| Protein sequence |
