AlfalfaGEDB | MS.gene064834

M. sativa cultivar XinJiangDaYe / MS.gene064834

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064834.t1	XP_013462271.1	97.7	222	5	0	1	222	1	222	4.50E-114	420.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064834.t1	I3SCR3	97.7	222	5	0	1	222	1	222	3.2e-114	420.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049596	MS.gene064834	0.821971	3.04E-53	-1.69E-46
MS.gene049989	MS.gene064834	0.848594	5.55E-60	-1.69E-46
MS.gene050297	MS.gene064834	0.847869	8.80E-60	-1.69E-46
MS.gene05124	MS.gene064834	0.836458	9.25E-57	-1.69E-46
MS.gene051629	MS.gene064834	0.845119	4.95E-59	-1.69E-46
MS.gene051816	MS.gene064834	0.835919	1.27E-56	-1.69E-46
MS.gene051955	MS.gene064834	0.865537	5.50E-65	-1.69E-46
MS.gene052143	MS.gene064834	0.818746	1.67E-52	-1.69E-46
MS.gene052308	MS.gene064834	0.847346	1.23E-59	-1.69E-46
MS.gene052367	MS.gene064834	0.877791	4.73E-69	-1.69E-46
MS.gene053173	MS.gene064834	0.845458	4.01E-59	-1.69E-46
MS.gene053348	MS.gene064834	0.82477	6.74E-54	-1.69E-46
MS.gene053416	MS.gene064834	0.837877	4.01E-57	-1.69E-46
MS.gene053417	MS.gene064834	0.837103	6.33E-57	-1.69E-46
MS.gene05369	MS.gene064834	0.80814	3.58E-50	-1.69E-46
MS.gene054551	MS.gene064834	0.846973	1.55E-59	-1.69E-46
MS.gene054552	MS.gene064834	0.821779	3.37E-53	-1.69E-46
MS.gene054593	MS.gene064834	0.834599	2.73E-56	-1.69E-46
MS.gene054633	MS.gene064834	0.807261	5.51E-50	-1.69E-46
MS.gene054643	MS.gene064834	0.803468	3.44E-49	-1.69E-46
MS.gene054662	MS.gene064834	0.815117	1.09E-51	-1.69E-46
MS.gene054885	MS.gene064834	0.817213	3.71E-52	-1.69E-46
MS.gene055308	MS.gene064834	0.839103	1.94E-57	-1.69E-46
MS.gene055578	MS.gene064834	0.856068	4.13E-62	-1.69E-46
MS.gene055579	MS.gene064834	0.801374	9.28E-49	-1.69E-46
MS.gene055957	MS.gene064834	0.849822	2.53E-60	-1.69E-46
MS.gene056041	MS.gene064834	0.885376	8.58E-72	-1.69E-46
MS.gene05640	MS.gene064834	0.856604	2.88E-62	-1.69E-46
MS.gene056526	MS.gene064834	0.816898	4.36E-52	-1.69E-46
MS.gene056528	MS.gene064834	0.811131	8.16E-51	-1.69E-46
MS.gene057458	MS.gene064834	0.858586	7.44E-63	-1.69E-46
MS.gene058013	MS.gene064834	0.812165	4.86E-51	-1.69E-46
MS.gene058128	MS.gene064834	0.806542	7.82E-50	-1.69E-46
MS.gene058818	MS.gene064834	0.811628	6.37E-51	-1.69E-46
MS.gene058827	MS.gene064834	0.817798	2.74E-52	-1.69E-46
MS.gene058844	MS.gene064834	0.810456	1.14E-50	-1.69E-46
MS.gene058853	MS.gene064834	0.844804	6.03E-59	-1.69E-46
MS.gene059300	MS.gene064834	0.816335	5.83E-52	-1.69E-46
MS.gene059553	MS.gene064834	0.862608	4.49E-64	-1.69E-46
MS.gene060473	MS.gene064834	0.887698	1.13E-72	-1.69E-46
MS.gene060565	MS.gene064834	0.87804	3.87E-69	-1.69E-46
MS.gene06057	MS.gene064834	0.816777	4.64E-52	-1.69E-46
MS.gene06093	MS.gene064834	0.852501	4.43E-61	-1.69E-46
MS.gene061099	MS.gene064834	0.861886	7.48E-64	-1.69E-46
MS.gene061260	MS.gene064834	0.882137	1.34E-70	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064834.t1	MTR_3g115300	97.748	222	5	0	1	222	1	222	7.65e-160	441

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064834.t1	AT5G42330	47.208	197	83	10	6	194	2	185	4.86e-37	129

Find 53 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCACAATTGCTTCTGAATTT+AGG	0.081710	3.4:+98648296	MS.gene064834:CDS
CACAATTGCTTCTGAATTTA+GGG	0.195473	3.4:+98648297	MS.gene064834:CDS
TGAAACAGAACACCAGATTT+AGG	0.204443	3.4:-98648394	None:intergenic
GAAGCAATTGTGCGGTATTC+AGG	0.265276	3.4:-98648286	None:intergenic
AGGATGGTTCGTGACGAATT+TGG	0.279654	3.4:+98648328	MS.gene064834:CDS
TCGGTCGCGGTGGTCGGAGG+TGG	0.289193	3.4:-98648040	None:intergenic
TGGTCGGAGGTGGGATTTCA+TGG	0.291163	3.4:-98648030	None:intergenic
GAAACAGAACACCAGATTTA+GGG	0.318085	3.4:-98648393	None:intergenic
CCAGATCCGGAAGAAATTAA+TGG	0.326179	3.4:+98648355	MS.gene064834:CDS
CCATTAATTTCTTCCGGATC+TGG	0.333300	3.4:-98648355	None:intergenic
TTTAACCAAGTCTCGGAAAC+TGG	0.342643	3.4:+98648243	MS.gene064834:CDS
AGATTTAGGGTTACAAGAAT+TGG	0.342948	3.4:-98648380	None:intergenic
CTTGGTGTTACTGTTGAATC+TGG	0.346379	3.4:+98648547	MS.gene064834:CDS
TTGGTACCATTAATTTCTTC+CGG	0.348296	3.4:-98648361	None:intergenic
ATGATGAGTATTCTGCAAAA+AGG	0.356084	3.4:+98648431	MS.gene064834:CDS
CGAAGACGCATCGGAGACTT+AGG	0.358488	3.4:-98648065	None:intergenic
CGGTATTCAGGTCGTAGTTC+AGG	0.361330	3.4:-98648274	None:intergenic
AATAATCATCATAATGTTCT+TGG	0.387259	3.4:+98648529	MS.gene064834:CDS
AATTCTTGTAACCCTAAATC+TGG	0.396987	3.4:+98648382	MS.gene064834:CDS
AAGAATACTGCAAGAAAACT+TGG	0.439698	3.4:+98648574	MS.gene064834:CDS
CCCTCTCTTCAAACTCCACC+AGG	0.453304	3.4:+98648110	MS.gene064834:CDS
GAGAGGTTGAAGAGAAAGAA+AGG	0.462625	3.4:+98648457	MS.gene064834:CDS
TAGGGATCGGTCGCGGTGGT+CGG	0.470489	3.4:-98648046	None:intergenic
TTGAACCAGTTTCCGAGACT+TGG	0.470567	3.4:-98648248	None:intergenic
CGGTCGCGGTGGTCGGAGGT+GGG	0.481693	3.4:-98648039	None:intergenic
CTGAATTTAGGGCTTTATCA+AGG	0.492327	3.4:+98648308	MS.gene064834:CDS
CGAATTTGGAAAACCAGATC+CGG	0.501998	3.4:+98648342	MS.gene064834:CDS
AATAAGAAACCTCCTCTAGC+TGG	0.510440	3.4:+98648682	MS.gene064834:CDS
TACTAGTATTAAGCAAACTA+AGG	0.520435	3.4:+98648504	MS.gene064834:CDS
GTTGGAGCATCGAGAAGAGT+AGG	0.523400	3.4:+98648745	MS.gene064834:CDS
ACTGTTGGTGAGAAGAAAGT+TGG	0.529021	3.4:+98648727	MS.gene064834:CDS
GGATCGGTCGCGGTGGTCGG+AGG	0.529657	3.4:-98648043	None:intergenic
ATTTAGGGCTTTATCAAGGA+TGG	0.529903	3.4:+98648312	MS.gene064834:CDS
CCTGGTGGAGTTTGAAGAGA+GGG	0.535099	3.4:-98648110	None:intergenic
CATGCTTAGAAGCATGACTA+AGG	0.538545	3.4:+98648657	MS.gene064834:CDS
CAGGTTCTTTAACCAAGTCT+CGG	0.545491	3.4:+98648236	MS.gene064834:intron
TTCTGCTGCTTATTCTGCTG+AGG	0.545660	3.4:+98648615	MS.gene064834:CDS
TTTGATAAATCTGTTACTGT+TGG	0.555181	3.4:+98648712	MS.gene064834:CDS
ACCTGGTGGAGTTTGAAGAG+AGG	0.555239	3.4:-98648111	None:intergenic
GAAGACGCATCGGAGACTTA+GGG	0.556071	3.4:-98648064	None:intergenic
TCAAATCTTGAACCAGCTAG+AGG	0.605228	3.4:-98648694	None:intergenic
CGCATCGGAGACTTAGGGAT+CGG	0.618390	3.4:-98648059	None:intergenic
ATGCTTCTAAGCATGTACCT+TGG	0.620137	3.4:-98648649	None:intergenic
AAGAGAGGGAGAGTCAAGAA+CGG	0.623596	3.4:-98648096	None:intergenic
GTTCGAGTTCGAAGACGCAT+CGG	0.624113	3.4:-98648074	None:intergenic
GGAGACTTAGGGATCGGTCG+CGG	0.628168	3.4:-98648053	None:intergenic
AATCTTGAACCAGCTAGAGG+AGG	0.634666	3.4:-98648691	None:intergenic
CTGAGGTTAGTGAAACTCCA+AGG	0.641822	3.4:+98648632	MS.gene064834:CDS
GACTTAGGGATCGGTCGCGG+TGG	0.645567	3.4:-98648050	None:intergenic
GAAGAGAAAGAAAGGAGTCA+CGG	0.667345	3.4:+98648465	MS.gene064834:CDS
TCTGGTGTTCTGTTTCAGAG+AGG	0.698623	3.4:+98648400	MS.gene064834:CDS
TAAATTCAGAAGCAATTGTG+CGG	0.713706	3.4:-98648294	None:intergenic
GAGAAAGAAAGGAGTCACGG+TGG	0.729465	3.4:+98648468	MS.gene064834:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATAATCATCATAATGTTCT+TGG	+	chr3.4:98648529-98648548	MS.gene064834:CDS	20.0%
!	GAAATAAGATTATAAGAACC+TGG	-	chr3.4:98648131-98648150	None:intergenic	25.0%
!	TACTAGTATTAAGCAAACTA+AGG	+	chr3.4:98648504-98648523	MS.gene064834:CDS	25.0%
!	TTTGATAAATCTGTTACTGT+TGG	+	chr3.4:98648712-98648731	MS.gene064834:CDS	25.0%
	AAGAATACTGCAAGAAAACT+TGG	+	chr3.4:98648574-98648593	MS.gene064834:CDS	30.0%
	AATTCTTGTAACCCTAAATC+TGG	+	chr3.4:98648382-98648401	MS.gene064834:CDS	30.0%
	ATAAGATTATAAGAACCTGG+TGG	-	chr3.4:98648128-98648147	None:intergenic	30.0%
	ATGATGAGTATTCTGCAAAA+AGG	+	chr3.4:98648431-98648450	MS.gene064834:CDS	30.0%
	CACAATTGCTTCTGAATTTA+GGG	+	chr3.4:98648297-98648316	MS.gene064834:CDS	30.0%
	CCACAAACAAAAAATGTCAA+TGG	-	chr3.4:98648201-98648220	None:intergenic	30.0%
	GTGGATTTGAAAATTGAAAC+AGG	+	chr3.4:98648217-98648236	MS.gene064834:intron	30.0%
	TAAATTCAGAAGCAATTGTG+CGG	-	chr3.4:98648297-98648316	None:intergenic	30.0%
	TTGGTACCATTAATTTCTTC+CGG	-	chr3.4:98648364-98648383	None:intergenic	30.0%
!	AGATTTAGGGTTACAAGAAT+TGG	-	chr3.4:98648383-98648402	None:intergenic	30.0%
!!!	CCATTGACATTTTTTGTTTG+TGG	+	chr3.4:98648198-98648217	MS.gene064834:intron	30.0%
	GAAACAGAACACCAGATTTA+GGG	-	chr3.4:98648396-98648415	None:intergenic	35.0%
	GCACAATTGCTTCTGAATTT+AGG	+	chr3.4:98648296-98648315	MS.gene064834:CDS	35.0%
	TGAAACAGAACACCAGATTT+AGG	-	chr3.4:98648397-98648416	None:intergenic	35.0%
!	ATTTAGGGCTTTATCAAGGA+TGG	+	chr3.4:98648312-98648331	MS.gene064834:CDS	35.0%
!	CTGAATTTAGGGCTTTATCA+AGG	+	chr3.4:98648308-98648327	MS.gene064834:CDS	35.0%
	AATAAGAAACCTCCTCTAGC+TGG	+	chr3.4:98648682-98648701	MS.gene064834:CDS	40.0%
	ATGCTTCTAAGCATGTACCT+TGG	-	chr3.4:98648652-98648671	None:intergenic	40.0%
	ATTCTGCAAAAAGGAACGAG+AGG	+	chr3.4:98648440-98648459	MS.gene064834:CDS	40.0%
	CAGGTTCTTTAACCAAGTCT+CGG	+	chr3.4:98648236-98648255	MS.gene064834:intron	40.0%
	CCAGATCCGGAAGAAATTAA+TGG	+	chr3.4:98648355-98648374	MS.gene064834:CDS	40.0%
	CCATTAATTTCTTCCGGATC+TGG	-	chr3.4:98648358-98648377	None:intergenic	40.0%
	CGAATTTGGAAAACCAGATC+CGG	+	chr3.4:98648342-98648361	MS.gene064834:CDS	40.0%
	GAAGAGAAAGAAAGGAGTCA+CGG	+	chr3.4:98648465-98648484	MS.gene064834:CDS	40.0%
	GAGAGGTTGAAGAGAAAGAA+AGG	+	chr3.4:98648457-98648476	MS.gene064834:CDS	40.0%
	TCAAATCTTGAACCAGCTAG+AGG	-	chr3.4:98648697-98648716	None:intergenic	40.0%
	TTTAACCAAGTCTCGGAAAC+TGG	+	chr3.4:98648243-98648262	MS.gene064834:CDS	40.0%
!	ACTGTTGGTGAGAAGAAAGT+TGG	+	chr3.4:98648727-98648746	MS.gene064834:CDS	40.0%
!	CATGCTTAGAAGCATGACTA+AGG	+	chr3.4:98648657-98648676	MS.gene064834:CDS	40.0%
!!	CTTGGTGTTACTGTTGAATC+TGG	+	chr3.4:98648547-98648566	MS.gene064834:CDS	40.0%
	AAGAGAGGGAGAGTCAAGAA+CGG	-	chr3.4:98648099-98648118	None:intergenic	45.0%
	AATCTTGAACCAGCTAGAGG+AGG	-	chr3.4:98648694-98648713	None:intergenic	45.0%
	AGGATGGTTCGTGACGAATT+TGG	+	chr3.4:98648328-98648347	MS.gene064834:CDS	45.0%
	CTGAGGTTAGTGAAACTCCA+AGG	+	chr3.4:98648632-98648651	MS.gene064834:CDS	45.0%
	GAAGCAATTGTGCGGTATTC+AGG	-	chr3.4:98648289-98648308	None:intergenic	45.0%
	TCTGGTGTTCTGTTTCAGAG+AGG	+	chr3.4:98648400-98648419	MS.gene064834:CDS	45.0%
	TTGAACCAGTTTCCGAGACT+TGG	-	chr3.4:98648251-98648270	None:intergenic	45.0%
!	TTCTGCTGCTTATTCTGCTG+AGG	+	chr3.4:98648615-98648634	MS.gene064834:CDS	45.0%
	ACCTGGTGGAGTTTGAAGAG+AGG	-	chr3.4:98648114-98648133	None:intergenic	50.0%
	CCTGGTGGAGTTTGAAGAGA+GGG	-	chr3.4:98648113-98648132	None:intergenic	50.0%
	CGGTATTCAGGTCGTAGTTC+AGG	-	chr3.4:98648277-98648296	None:intergenic	50.0%
	GAAGACGCATCGGAGACTTA+GGG	-	chr3.4:98648067-98648086	None:intergenic	50.0%
	GAGAAAGAAAGGAGTCACGG+TGG	+	chr3.4:98648468-98648487	MS.gene064834:CDS	50.0%
!	GTTCGAGTTCGAAGACGCAT+CGG	-	chr3.4:98648077-98648096	None:intergenic	50.0%
!!	GTTGGAGCATCGAGAAGAGT+AGG	+	chr3.4:98648745-98648764	MS.gene064834:CDS	50.0%
	CCCTCTCTTCAAACTCCACC+AGG	+	chr3.4:98648110-98648129	MS.gene064834:CDS	55.0%
	CGAAGACGCATCGGAGACTT+AGG	-	chr3.4:98648068-98648087	None:intergenic	55.0%
	CGCATCGGAGACTTAGGGAT+CGG	-	chr3.4:98648062-98648081	None:intergenic	55.0%
	TGGTCGGAGGTGGGATTTCA+TGG	-	chr3.4:98648033-98648052	None:intergenic	55.0%
	GGAGACTTAGGGATCGGTCG+CGG	-	chr3.4:98648056-98648075	None:intergenic	60.0%
	GACTTAGGGATCGGTCGCGG+TGG	-	chr3.4:98648053-98648072	None:intergenic	65.0%
	TAGGGATCGGTCGCGGTGGT+CGG	-	chr3.4:98648049-98648068	None:intergenic	65.0%
	CGGTCGCGGTGGTCGGAGGT+GGG	-	chr3.4:98648042-98648061	None:intergenic	75.0%
	GGATCGGTCGCGGTGGTCGG+AGG	-	chr3.4:98648046-98648065	None:intergenic	75.0%
	TCGGTCGCGGTGGTCGGAGG+TGG	-	chr3.4:98648043-98648062	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	98648011	98648786	98648011	ID=MS.gene064834
chr3.4	mRNA	98648011	98648786	98648011	ID=MS.gene064834.t1;Parent=MS.gene064834
chr3.4	exon	98648011	98648131	98648011	ID=MS.gene064834.t1.exon1;Parent=MS.gene064834.t1
chr3.4	CDS	98648011	98648131	98648011	ID=cds.MS.gene064834.t1;Parent=MS.gene064834.t1
chr3.4	exon	98648239	98648786	98648239	ID=MS.gene064834.t1.exon2;Parent=MS.gene064834.t1
chr3.4	CDS	98648239	98648786	98648239	ID=cds.MS.gene064834.t1;Parent=MS.gene064834.t1

Gene Sequence

>MS.gene064834

ATGTACTATTCATCATCAACCATGAAATCCCACCTCCGACCACCGCGACCGATCCCTAAGTCTCCGATGCGTCTTCGAACTCGAACCGTTCTTGACTCTCCCTCTCTTCAAACTCCACCAGGTTCTTATAATCTTATTTCTAATTAATTCTTCTACTTTAATTTCTCGATTTTATATTTTAATTTCTCCATTGACATTTTTTGTTTGTGGATTTGAAAATTGAAACAGGTTCTTTAACCAAGTCTCGGAAACTGGTTCAATCTCCTGAACTACGACCTGAATACCGCACAATTGCTTCTGAATTTAGGGCTTTATCAAGGATGGTTCGTGACGAATTTGGAAAACCAGATCCGGAAGAAATTAATGGTACCAATTCTTGTAACCCTAAATCTGGTGTTCTGTTTCAGAGAGGAAGATTCTATGATGAGTATTCTGCAAAAAGGAACGAGAGGTTGAAGAGAAAGAAAGGAGTCACGGTGGATGAAGTTAATACTACTAGTATTAAGCAAACTAAGGTGAATAATCATCATAATGTTCTTGGTGTTACTGTTGAATCTGGAAAAAAGAATACTGCAAGAAAACTTGGAAGTTTGAGAAAATCTGTTTCTGCTGCTTATTCTGCTGAGGTTAGTGAAACTCCAAGGTACATGCTTAGAAGCATGACTAAGGAGAATAAGAAACCTCCTCTAGCTGGTTCAAGATTTGATAAATCTGTTACTGTTGGTGAGAAGAAAGTTGGAGCATCGAGAAGAGTAGGAAAAATTTCGTACTATTAG

Protein sequence

>MS.gene064834.t1

MYYSSSTMKSHLRPPRPIPKSPMRLRTRTVLDSPSLQTPPGSLTKSRKLVQSPELRPEYRTIASEFRALSRMVRDEFGKPDPEEINGTNSCNPKSGVLFQRGRFYDEYSAKRNERLKRKKGVTVDEVNTTSIKQTKVNNHHNVLGVTVESGKKNTARKLGSLRKSVSAAYSAEVSETPRYMLRSMTKENKKPPLAGSRFDKSVTVGEKKVGASRRVGKISYY