AlfalfaGEDB | MS.gene065267

M. sativa cultivar XinJiangDaYe / MS.gene065267

KEGG: -
GO: -
PFAM: PF04935.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065267.t1	KEH38713.1	61	187	63	2	5	191	49	225	1.60E-40	176

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065267.t1	A0A072VB89	61.0	187	63	2	5	191	49	225	1.2e-40	176.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene065267	0.804172	2.45E-49	-1.69E-46
MS.gene050227	MS.gene065267	0.813625	2.33E-51	-1.69E-46
MS.gene050228	MS.gene065267	0.817105	3.92E-52	-1.69E-46
MS.gene050303	MS.gene065267	0.81547	9.09E-52	-1.69E-46
MS.gene050330	MS.gene065267	-0.813496	2.49E-51	-1.69E-46
MS.gene050442	MS.gene065267	0.807908	4.02E-50	-1.69E-46
MS.gene050719	MS.gene065267	0.826575	2.52E-54	-1.69E-46
MS.gene050721	MS.gene065267	0.825135	5.53E-54	-1.69E-46
MS.gene050724	MS.gene065267	0.81309	3.05E-51	-1.69E-46
MS.gene050780	MS.gene065267	0.803889	2.81E-49	-1.69E-46
MS.gene050815	MS.gene065267	0.804636	1.96E-49	-1.69E-46
MS.gene050824	MS.gene065267	0.814376	1.59E-51	-1.69E-46
MS.gene050844	MS.gene065267	0.804027	2.63E-49	-1.69E-46
MS.gene051265	MS.gene065267	0.805213	1.49E-49	-1.69E-46
MS.gene052012	MS.gene065267	0.807582	4.71E-50	-1.69E-46
MS.gene052176	MS.gene065267	0.805536	1.27E-49	-1.69E-46
MS.gene052190	MS.gene065267	0.819472	1.14E-52	-1.69E-46
MS.gene052309	MS.gene065267	-0.813616	2.34E-51	-1.69E-46
MS.gene052358	MS.gene065267	-0.82207	2.88E-53	-1.69E-46
MS.gene052519	MS.gene065267	0.807063	6.07E-50	-1.69E-46
MS.gene052863	MS.gene065267	0.819787	9.67E-53	-1.69E-46
MS.gene052896	MS.gene065267	0.819529	1.11E-52	-1.69E-46
MS.gene053006	MS.gene065267	0.804405	2.19E-49	-1.69E-46
MS.gene053008	MS.gene065267	0.81818	2.24E-52	-1.69E-46
MS.gene054468	MS.gene065267	0.801694	7.98E-49	-1.69E-46
MS.gene054472	MS.gene065267	0.805651	1.20E-49	-1.69E-46
MS.gene055358	MS.gene065267	0.808203	3.48E-50	-1.69E-46
MS.gene055818	MS.gene065267	0.842719	2.18E-58	-1.69E-46
MS.gene056506	MS.gene065267	0.828328	9.56E-55	-1.69E-46
MS.gene057026	MS.gene065267	0.824945	6.13E-54	-1.69E-46
MS.gene057060	MS.gene065267	0.804425	2.17E-49	-1.69E-46
MS.gene058037	MS.gene065267	0.809102	2.23E-50	-1.69E-46
MS.gene058139	MS.gene065267	0.800973	1.12E-48	-1.69E-46
MS.gene058399	MS.gene065267	0.823139	1.63E-53	-1.69E-46
MS.gene058625	MS.gene065267	0.803674	3.11E-49	-1.69E-46
MS.gene058906	MS.gene065267	0.809239	2.09E-50	-1.69E-46
MS.gene059249	MS.gene065267	0.814305	1.65E-51	-1.69E-46
MS.gene059789	MS.gene065267	0.961038	3.06E-119	-1.69E-46
MS.gene059830	MS.gene065267	0.806765	7.02E-50	-1.69E-46
MS.gene059842	MS.gene065267	0.801086	1.06E-48	-1.69E-46
MS.gene060322	MS.gene065267	0.851819	6.92E-61	-1.69E-46
MS.gene060572	MS.gene065267	0.835052	2.10E-56	-1.69E-46
MS.gene060683	MS.gene065267	0.829581	4.75E-55	-1.69E-46
MS.gene060685	MS.gene065267	0.827563	1.46E-54	-1.69E-46
MS.gene060803	MS.gene065267	0.813605	2.35E-51	-1.69E-46
MS.gene061040	MS.gene065267	-0.839277	1.74E-57	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065267.t1	MTR_2g079000	79.021	143	28	1	49	191	85	225	2.24e-68	208
MS.gene065267.t1	MTR_5g012820	61.929	197	48	4	10	179	109	305	2.42e-65	204
MS.gene065267.t1	MTR_5g012820	61.929	197	48	4	10	179	119	315	4.75e-65	204
MS.gene065267.t1	MTR_2g079040	87.500	40	5	0	133	172	1	40	7.42e-16	71.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065267.t1	AT5G05210	46.078	204	78	6	1	179	144	340	1.25e-41	144
MS.gene065267.t1	AT5G05210	46.078	204	78	6	1	179	136	332	1.25e-41	144
MS.gene065267.t1	AT2G27750	37.356	174	75	6	12	179	3	148	5.84e-21	85.9

Find 44 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGGTGAAGCTATTGCTAAAA+AGG	0.287342	2.4:+19341222	MS.gene065267:CDS
ATACTTCTCTTTATCAGTTT+AGG	0.301257	2.4:-19341298	None:intergenic
TGAAAGAAAGATAGAGTTTC+CGG	0.308185	2.4:+19341036	MS.gene065267:CDS
AGGTCCTAACTGTGCAAATC+TGG	0.332531	2.4:+19340994	MS.gene065267:CDS
GCAGTTAAGCCTTTCATGTC+GGG	0.372922	2.4:+19340893	MS.gene065267:CDS
AGCAGAAAATCGCAGTGAAA+TGG	0.374864	2.4:+19341341	MS.gene065267:CDS
TGAAAGAGCTAAGAAGTTAG+AGG	0.400614	2.4:+19341174	MS.gene065267:CDS
GTATACAGAAATGGAAGAAT+AGG	0.408150	2.4:+19341317	MS.gene065267:CDS
GCAATAGCTTCACCCTTCTC+AGG	0.419537	2.4:-19341214	None:intergenic
AGCAGCAATGAATAGAGCTT+CGG	0.425070	2.4:+19341255	MS.gene065267:CDS
GCAGCAATGAATAGAGCTTC+GGG	0.426903	2.4:+19341256	MS.gene065267:CDS
TCTTTAAAAGAGCTTGTGTT+TGG	0.437000	2.4:+19341088	MS.gene065267:CDS
TCCTTTACCACCTTCTCCTC+CGG	0.440386	2.4:-19341055	None:intergenic
ATCTGGAAAATGCTAATAAG+AGG	0.444036	2.4:+19341011	MS.gene065267:CDS
TAAAGAGAAGTATACAGAAA+TGG	0.461758	2.4:+19341308	MS.gene065267:CDS
GCAGAAAATCGCAGTGAAAT+GGG	0.472714	2.4:+19341342	MS.gene065267:CDS
TCTTTCAAGTTCCTTGTGCT+TGG	0.476271	2.4:-19341158	None:intergenic
GGCAATGTTAAACTTCAGAA+TGG	0.487362	2.4:+19341109	MS.gene065267:CDS
GATAGAGTTTCCGGAGGAGA+AGG	0.496889	2.4:+19341045	MS.gene065267:CDS
GAATAGAGCTTCGGGAATCA+AGG	0.497557	2.4:+19341264	MS.gene065267:CDS
TCCGGAGGAGAAGGTGGTAA+AGG	0.513098	2.4:+19341054	MS.gene065267:CDS
TCAAACAAAGGATCAGTTGA+AGG	0.525525	2.4:+19341382	MS.gene065267:CDS
AAACTTGAAGAGTTTCAGTC+AGG	0.526291	2.4:+19340974	MS.gene065267:CDS
TAAGCCTTTCATGTCGGGGT+TGG	0.538275	2.4:+19340898	MS.gene065267:CDS
TGCAGTTAAGCCTTTCATGT+CGG	0.538494	2.4:+19340892	MS.gene065267:CDS
CCTTTCGCGTAGTTCTTCAA+AGG	0.546785	2.4:-19340942	None:intergenic
CGCAGTGAAATGGGAAGAGA+GGG	0.547718	2.4:+19341351	MS.gene065267:CDS
GCTTCCAACCCCGACATGAA+AGG	0.556004	2.4:-19340902	None:intergenic
CGGGGTTGGAAGCAGATGAT+CGG	0.561569	2.4:+19340912	MS.gene065267:CDS
CTTCAGAATGGTGAAATGCA+AGG	0.562609	2.4:+19341121	MS.gene065267:CDS
TTCAGAATGGTGAAATGCAA+GGG	0.568164	2.4:+19341122	MS.gene065267:CDS
AGAGTTTCCGGAGGAGAAGG+TGG	0.570130	2.4:+19341048	MS.gene065267:CDS
AATGACAGCGATGGAGAGGA+AGG	0.580649	2.4:+19340869	MS.gene065267:CDS
AATGAATGACAGCGATGGAG+AGG	0.585376	2.4:+19340865	None:intergenic
GAAGAAGAATGATCCTGAGA+AGG	0.589096	2.4:+19341201	MS.gene065267:CDS
AAGAAGAATGATCCTGAGAA+GGG	0.591026	2.4:+19341202	MS.gene065267:CDS
CAGTTAAGCCTTTCATGTCG+GGG	0.594657	2.4:+19340894	MS.gene065267:CDS
TCTGGAAAATGCTAATAAGA+GGG	0.594774	2.4:+19341012	MS.gene065267:CDS
TCGCAGTGAAATGGGAAGAG+AGG	0.597560	2.4:+19341350	MS.gene065267:CDS
CCTTTGAAGAACTACGCGAA+AGG	0.632563	2.4:+19340942	MS.gene065267:CDS
AGGGTTCAAAGTTCAAACAA+AGG	0.644899	2.4:+19341370	MS.gene065267:CDS
GAGAAAAGTTTCCAAGCACA+AGG	0.645702	2.4:+19341147	MS.gene065267:CDS
GGAGGAGAAGGTGGTAAAGG+AGG	0.646856	2.4:+19341057	MS.gene065267:CDS
AAGAAAGATAGAGTTTCCGG+AGG	0.655190	2.4:+19341039	MS.gene065267:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATACTTCTCTTTATCAGTTT+AGG	-	chr2.4:19341301-19341320	None:intergenic	25.0%
!!	TAAAGAGAAGTATACAGAAA+TGG	+	chr2.4:19341308-19341327	MS.gene065267:CDS	25.0%
	ATCTGGAAAATGCTAATAAG+AGG	+	chr2.4:19341011-19341030	MS.gene065267:CDS	30.0%
	GTATACAGAAATGGAAGAAT+AGG	+	chr2.4:19341317-19341336	MS.gene065267:CDS	30.0%
	TCTGGAAAATGCTAATAAGA+GGG	+	chr2.4:19341012-19341031	MS.gene065267:CDS	30.0%
	TGAAAGAAAGATAGAGTTTC+CGG	+	chr2.4:19341036-19341055	MS.gene065267:CDS	30.0%
!	TCTTTAAAAGAGCTTGTGTT+TGG	+	chr2.4:19341088-19341107	MS.gene065267:CDS	30.0%
	AAACTTGAAGAGTTTCAGTC+AGG	+	chr2.4:19340974-19340993	MS.gene065267:CDS	35.0%
	AAGAAGAATGATCCTGAGAA+GGG	+	chr2.4:19341202-19341221	MS.gene065267:CDS	35.0%
	AGGCAGAGAAACAACAAAAA+AGG	+	chr2.4:19341402-19341421	MS.gene065267:CDS	35.0%
	AGGGTTCAAAGTTCAAACAA+AGG	+	chr2.4:19341370-19341389	MS.gene065267:CDS	35.0%
	CTATTGCTAAAAAGGAAGCA+TGG	+	chr2.4:19341230-19341249	MS.gene065267:CDS	35.0%
	GGCAATGTTAAACTTCAGAA+TGG	+	chr2.4:19341109-19341128	MS.gene065267:CDS	35.0%
	TCAAACAAAGGATCAGTTGA+AGG	+	chr2.4:19341382-19341401	MS.gene065267:CDS	35.0%
	TGAAAGAGCTAAGAAGTTAG+AGG	+	chr2.4:19341174-19341193	MS.gene065267:CDS	35.0%
	TTCAGAATGGTGAAATGCAA+GGG	+	chr2.4:19341122-19341141	MS.gene065267:CDS	35.0%
!	TTTTCCAGATTTGCACAGTT+AGG	-	chr2.4:19341001-19341020	None:intergenic	35.0%
!!	AAGCTCTTTTAAAGACTCTG+TGG	-	chr2.4:19341083-19341102	None:intergenic	35.0%
	AAGAAAGATAGAGTTTCCGG+AGG	+	chr2.4:19341039-19341058	MS.gene065267:CDS	40.0%
	AGCAGAAAATCGCAGTGAAA+TGG	+	chr2.4:19341341-19341360	MS.gene065267:CDS	40.0%
	AGCAGCAATGAATAGAGCTT+CGG	+	chr2.4:19341255-19341274	MS.gene065267:CDS	40.0%
	CTTCAGAATGGTGAAATGCA+AGG	+	chr2.4:19341121-19341140	MS.gene065267:CDS	40.0%
	GAAGAAGAATGATCCTGAGA+AGG	+	chr2.4:19341201-19341220	MS.gene065267:CDS	40.0%
	GAGAAAAGTTTCCAAGCACA+AGG	+	chr2.4:19341147-19341166	MS.gene065267:CDS	40.0%
	GCAGAAAATCGCAGTGAAAT+GGG	+	chr2.4:19341342-19341361	MS.gene065267:CDS	40.0%
	GGGTGAAGCTATTGCTAAAA+AGG	+	chr2.4:19341222-19341241	MS.gene065267:CDS	40.0%
	TGCAGTTAAGCCTTTCATGT+CGG	+	chr2.4:19340892-19340911	MS.gene065267:CDS	40.0%
!!	TCTTTCAAGTTCCTTGTGCT+TGG	-	chr2.4:19341161-19341180	None:intergenic	40.0%
	AGGTCCTAACTGTGCAAATC+TGG	+	chr2.4:19340994-19341013	MS.gene065267:CDS	45.0%
	CAGTTAAGCCTTTCATGTCG+GGG	+	chr2.4:19340894-19340913	MS.gene065267:CDS	45.0%
	CCTTTCGCGTAGTTCTTCAA+AGG	-	chr2.4:19340945-19340964	None:intergenic	45.0%
	GAATAGAGCTTCGGGAATCA+AGG	+	chr2.4:19341264-19341283	MS.gene065267:CDS	45.0%
	GCAGCAATGAATAGAGCTTC+GGG	+	chr2.4:19341256-19341275	MS.gene065267:CDS	45.0%
	GCAGTTAAGCCTTTCATGTC+GGG	+	chr2.4:19340893-19340912	MS.gene065267:CDS	45.0%
!	CCTTTGAAGAACTACGCGAA+AGG	+	chr2.4:19340942-19340961	MS.gene065267:CDS	45.0%
	AATGACAGCGATGGAGAGGA+AGG	+	chr2.4:19340869-19340888	MS.gene065267:CDS	50.0%
	CGCAGTGAAATGGGAAGAGA+GGG	+	chr2.4:19341351-19341370	MS.gene065267:CDS	50.0%
	GATAGAGTTTCCGGAGGAGA+AGG	+	chr2.4:19341045-19341064	MS.gene065267:CDS	50.0%
	GCAATAGCTTCACCCTTCTC+AGG	-	chr2.4:19341217-19341236	None:intergenic	50.0%
	TAAGCCTTTCATGTCGGGGT+TGG	+	chr2.4:19340898-19340917	MS.gene065267:CDS	50.0%
	TCCTTTACCACCTTCTCCTC+CGG	-	chr2.4:19341058-19341077	None:intergenic	50.0%
	TCGCAGTGAAATGGGAAGAG+AGG	+	chr2.4:19341350-19341369	MS.gene065267:CDS	50.0%
	AGAGTTTCCGGAGGAGAAGG+TGG	+	chr2.4:19341048-19341067	MS.gene065267:CDS	55.0%
	CGGGGTTGGAAGCAGATGAT+CGG	+	chr2.4:19340912-19340931	MS.gene065267:CDS	55.0%
	GCTTCCAACCCCGACATGAA+AGG	-	chr2.4:19340905-19340924	None:intergenic	55.0%
	GGAGGAGAAGGTGGTAAAGG+AGG	+	chr2.4:19341057-19341076	MS.gene065267:CDS	55.0%
	TCCGGAGGAGAAGGTGGTAA+AGG	+	chr2.4:19341054-19341073	MS.gene065267:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	19340866	19341441	19340866	ID=MS.gene065267
chr2.4	mRNA	19340866	19341441	19340866	ID=MS.gene065267.t1;Parent=MS.gene065267
chr2.4	exon	19340866	19341441	19340866	ID=MS.gene065267.t1.exon1;Parent=MS.gene065267.t1
chr2.4	CDS	19340866	19341441	19340866	ID=cds.MS.gene065267.t1;Parent=MS.gene065267.t1

Gene Sequence

>MS.gene065267

ATGAATGACAGCGATGGAGAGGAAGGTGCAGTTAAGCCTTTCATGTCGGGGTTGGAAGCAGATGATCGGTTTGCGACCTTTGAAGAACTACGCGAAAGGCTTCATCGCAAACTTGAAGAGTTTCAGTCAGGTCCTAACTGTGCAAATCTGGAAAATGCTAATAAGAGGGATGAAAGAAAGATAGAGTTTCCGGAGGAGAAGGTGGTAAAGGAGGCCACAGAGTCTTTAAAAGAGCTTGTGTTTGGCAATGTTAAACTTCAGAATGGTGAAATGCAAGGGAAGAGAAAAGTTTCCAAGCACAAGGAACTTGAAAGAGCTAAGAAGTTAGAGGAAGTGAAGAAGAATGATCCTGAGAAGGGTGAAGCTATTGCTAAAAAGGAAGCATGGAAAGCAGCAATGAATAGAGCTTCGGGAATCAAGGTTCATGATGATCCTAAACTGATAAAGAGAAGTATACAGAAATGGAAGAATAGGCAGCAGAAAATCGCAGTGAAATGGGAAGAGAGGGTTCAAAGTTCAAACAAAGGATCAGTTGAAGGCAGAGAAACAACAAAAAAGGTATGCAAATATAGCTGA

Protein sequence

>MS.gene065267.t1

MNDSDGEEGAVKPFMSGLEADDRFATFEELRERLHRKLEEFQSGPNCANLENANKRDERKIEFPEEKVVKEATESLKELVFGNVKLQNGEMQGKRKVSKHKELERAKKLEEVKKNDPEKGEAIAKKEAWKAAMNRASGIKVHDDPKLIKRSIQKWKNRQQKIAVKWEERVQSSNKGSVEGRETTKKVCKYS