AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08427


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 17 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTGCACCGTTAAGAAAA+AGG 0.287608 4.2:+14318048 MS.gene08427:CDS
TGTACCTCTGTGGAACAGTT+TGG 0.321117 4.2:-14316210 None:intergenic
TCCTTTACTTGCTGAAATGA+TGG 0.374631 4.2:+14316352 MS.gene08427:CDS
GTACCTCTGTGGAACAGTTT+GGG 0.391331 4.2:-14316209 None:intergenic
TAACGGTGCAGCAGTGTCTC+TGG 0.427524 4.2:-14318039 None:intergenic
TGCAGCAGTGTCTCTGGAAT+CGG 0.454691 4.2:-14318033 None:intergenic
AATTAATTTGAACTCAATCA+AGG 0.455044 4.2:+14316382 MS.gene08427:CDS
CCTTTACTTGCTGAAATGAT+GGG 0.538147 4.2:+14316353 MS.gene08427:CDS
CCCATCATTTCAGCAAGTAA+AGG 0.541167 4.2:-14316353 None:intergenic
ACGAAAACTCATCGAGGAGA+AGG 0.547378 4.2:+14318004 MS.gene08427:CDS
TACATCACTATCACAATAGC+TGG 0.549893 4.2:-14316166 None:intergenic
AGCTATTGTGATAGTGATGT+AGG 0.582124 4.2:+14316168 MS.gene08427:CDS
AGTGATGTAGGAGAGTTCAC+TGG 0.605602 4.2:+14316180 MS.gene08427:CDS
AAGGGATTATTGTACCTCTG+TGG 0.666642 4.2:-14316220 None:intergenic
GGATGCAGTTATGAATCTAG+AGG 0.670936 4.2:+14316143 None:intergenic
TAACCCAAACTGTTCCACAG+AGG 0.736648 4.2:+14316206 MS.gene08427:CDS
GGTTGAACGAAAACTCATCG+AGG 0.759935 4.2:+14317998 MS.gene08427:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATAAAATGAAATTGA+GGG - chr4.2:14317939-14317958 None:intergenic 10.0%
!! AATCTAAAAATTAACAAAAT+AGG + chr4.2:14317575-14317594 MS.gene08427:intron 10.0%
!! AATGTGTTAAATTAAAAATA+AGG + chr4.2:14317545-14317564 MS.gene08427:intron 10.0%
!! ATTAAGTAAATATAATCTTA+GGG - chr4.2:14316850-14316869 None:intergenic 10.0%
!! GAAAAAATAAAAATAAAAAG+TGG + chr4.2:14317890-14317909 MS.gene08427:intron 10.0%
!! TAATAAATAAAATGAAATTG+AGG - chr4.2:14317940-14317959 None:intergenic 10.0%
!!! ACAATTCTTTTATTTTATAT+GGG + chr4.2:14317362-14317381 MS.gene08427:intron 10.0%
!!! TTTTGTTAATTTTTAGATTT+TGG - chr4.2:14317574-14317593 None:intergenic 10.0%
!! CATTAAGTAAATATAATCTT+AGG - chr4.2:14316851-14316870 None:intergenic 15.0%
!!! AAAACAATAATTTGTTTCTT+AGG - chr4.2:14317713-14317732 None:intergenic 15.0%
!!! AAACAATAATTTGTTTCTTA+GGG - chr4.2:14317712-14317731 None:intergenic 15.0%
!!! ACTTATTTTACTTAAATCTT+AGG - chr4.2:14317622-14317641 None:intergenic 15.0%
!!! CAATTCTTTTATTTTATATG+GGG + chr4.2:14317363-14317382 MS.gene08427:intron 15.0%
!!! GACAATTCTTTTATTTTATA+TGG + chr4.2:14317361-14317380 MS.gene08427:intron 15.0%
!!! GACTGAATTTTATATTAAAA+TGG + chr4.2:14316870-14316889 MS.gene08427:intron 15.0%
!!! TATGTAATTTCAAGTATTAT+GGG + chr4.2:14316992-14317011 MS.gene08427:intron 15.0%
!!! TTAAATTACTATTGTTTTAG+TGG + chr4.2:14317291-14317310 MS.gene08427:intron 15.0%
!!! TTATGTAATTTCAAGTATTA+TGG + chr4.2:14316991-14317010 MS.gene08427:intron 15.0%
!! ATTTCTTCAATTCTATTGAA+TGG + chr4.2:14316778-14316797 MS.gene08427:intron 20.0%
!! CTAAAAATTAACAAAATAGG+AGG + chr4.2:14317578-14317597 MS.gene08427:intron 20.0%
!! GAATTCTACATAATTTACAT+CGG - chr4.2:14316563-14316582 None:intergenic 20.0%
!! TTTGTGATATTAATCAATGA+TGG + chr4.2:14317339-14317358 MS.gene08427:intron 20.0%
!!! AACAATAATTTGTTTCTTAG+GGG - chr4.2:14317711-14317730 None:intergenic 20.0%
!!! AATTAATTTGAACTCAATCA+AGG + chr4.2:14316382-14316401 MS.gene08427:CDS 20.0%
!!! ACAAATAAAAGCAAGATTTT+TGG - chr4.2:14317324-14317343 None:intergenic 20.0%
!!! GCAGATTATTGATTTTAATT+TGG + chr4.2:14316319-14316338 MS.gene08427:intron 20.0%
!!! TATTGATTTTAATTTGGATG+TGG + chr4.2:14316325-14316344 MS.gene08427:CDS 20.0%
!!! TCTAAAGGTTTCTTATTTTA+TGG + chr4.2:14316750-14316769 MS.gene08427:intron 20.0%
!!! TTTTGAACAAACCAAAATAA+AGG - chr4.2:14317525-14317544 None:intergenic 20.0%
! AAAAAAAGTAAAACGAGAAG+AGG + chr4.2:14317868-14317887 MS.gene08427:intron 25.0%
! AATGTGTTACCAAACAATAA+GGG - chr4.2:14316241-14316260 None:intergenic 25.0%
! ATTCTAGATGACTGTAATTA+CGG + chr4.2:14316579-14316598 MS.gene08427:intron 25.0%
! TACAATAATCCCTTATTGTT+TGG + chr4.2:14316229-14316248 MS.gene08427:intron 25.0%
! TATTGTATGGATGACTTATT+TGG + chr4.2:14317202-14317221 MS.gene08427:intron 25.0%
! TTAAAAAACCTTGTCATGAA+TGG + chr4.2:14316824-14316843 MS.gene08427:intron 25.0%
! TTGATTTCAATGAAATGTGT+TGG + chr4.2:14316265-14316284 MS.gene08427:intron 25.0%
! TTGTTTATAGGGAAAAATCA+TGG + chr4.2:14316903-14316922 MS.gene08427:intron 25.0%
!! ATTTTATGTAGCTTTTGTCT+AGG + chr4.2:14317977-14317996 MS.gene08427:intron 25.0%
!!! ACAATAATTTGTTTCTTAGG+GGG - chr4.2:14317710-14317729 None:intergenic 25.0%
!!! GGTTTTTTTTTTCCAAGAAA+AGG + chr4.2:14317240-14317259 MS.gene08427:intron 25.0%
!!! GTTTATTAGCACCTTTATTT+TGG + chr4.2:14317511-14317530 MS.gene08427:intron 25.0%
ATAGGGAAAAATCATGGTTA+TGG + chr4.2:14316909-14316928 MS.gene08427:intron 30.0%
ATTTGGAAAGAGAGAAATTC+TGG + chr4.2:14317219-14317238 MS.gene08427:intron 30.0%
CAATGTGTTACCAAACAATA+AGG - chr4.2:14316242-14316261 None:intergenic 30.0%
CATTGTGAAGTTTATATTGC+TGG + chr4.2:14316602-14316621 MS.gene08427:intron 30.0%
TGAATGGCCTAATGAAAAAT+GGG + chr4.2:14316794-14316813 MS.gene08427:intron 30.0%
TGGTTTCTTAAAATATCAGC+AGG + chr4.2:14316929-14316948 MS.gene08427:intron 30.0%
TTGAATGGCCTAATGAAAAA+TGG + chr4.2:14316793-14316812 MS.gene08427:intron 30.0%
! GACTCTCAAGTTTGTTTATA+GGG + chr4.2:14316892-14316911 MS.gene08427:intron 30.0%
!! AAAGGTGCTAATAAACCAAA+AGG - chr4.2:14317507-14317526 None:intergenic 30.0%
!! GTGTAAGTTTTATGCTAAGA+AGG + chr4.2:14317481-14317500 MS.gene08427:intron 30.0%
AAACCGACAGTTAAAACAGT+CGG + chr4.2:14317079-14317098 MS.gene08427:intron 35.0%
AAATGATGCAAACACACGAA+AGG - chr4.2:14317438-14317457 None:intergenic 35.0%
ACAGTTAAAACAGTCGGAAT+TGG + chr4.2:14317085-14317104 MS.gene08427:intron 35.0%
ACCATAATTGTCATTGCACT+AGG - chr4.2:14316653-14316672 None:intergenic 35.0%
ATAAGAAACCTTTAGACCCA+TGG - chr4.2:14316746-14316765 None:intergenic 35.0%
CTTAGCATAAAACTTACACG+AGG - chr4.2:14317480-14317499 None:intergenic 35.0%
TACATCACTATCACAATAGC+TGG - chr4.2:14316169-14316188 None:intergenic 35.0%
TTCGGTTTATTCGGTTAAGA+CGG - chr4.2:14317052-14317071 None:intergenic 35.0%
TTGTTCGGTTTCGGTTTATT+CGG - chr4.2:14317061-14317080 None:intergenic 35.0%
TTTAACCTTGTTGAACAACC+TGG - chr4.2:14317276-14317295 None:intergenic 35.0%
! AGCTATTGTGATAGTGATGT+AGG + chr4.2:14316168-14316187 MS.gene08427:CDS 35.0%
! ATACAATATGCCTTCGAGTT+AGG - chr4.2:14317190-14317209 None:intergenic 35.0%
! CCTTTACTTGCTGAAATGAT+GGG + chr4.2:14316353-14316372 MS.gene08427:CDS 35.0%
! GGACTCTCAAGTTTGTTTAT+AGG + chr4.2:14316891-14316910 MS.gene08427:intron 35.0%
! TAACTCGAAGGCATATTGTA+TGG + chr4.2:14317189-14317208 MS.gene08427:intron 35.0%
! TACAATATGCCTTCGAGTTA+GGG - chr4.2:14317189-14317208 None:intergenic 35.0%
! TCCTTTACTTGCTGAAATGA+TGG + chr4.2:14316352-14316371 MS.gene08427:CDS 35.0%
! TTTATGCTAAGAAGGCCTTT+TGG + chr4.2:14317489-14317508 MS.gene08427:intron 35.0%
!! ACCTATGCCCATTTTTCATT+AGG - chr4.2:14316804-14316823 None:intergenic 35.0%
!!! ATTCCGACTGTTTTAACTGT+CGG - chr4.2:14317085-14317104 None:intergenic 35.0%
!!! GTTTTAACTGTCGGTTTGTT+CGG - chr4.2:14317076-14317095 None:intergenic 35.0%
AAGGGATTATTGTACCTCTG+TGG - chr4.2:14316223-14316242 None:intergenic 40.0%
ACATAGTGCATACTGCATAC+TGG - chr4.2:14316713-14316732 None:intergenic 40.0%
CAATTGCTGATGCTACAACA+TGG - chr4.2:14316527-14316546 None:intergenic 40.0%
CACACGAAAGGTAACAAAAC+TGG - chr4.2:14317426-14317445 None:intergenic 40.0%
CCCATCATTTCAGCAAGTAA+AGG - chr4.2:14316356-14316375 None:intergenic 40.0%
GCCTAATGAAAAATGGGCAT+AGG + chr4.2:14316800-14316819 MS.gene08427:intron 40.0%
GCCTAGTGCAATGACAATTA+TGG + chr4.2:14316649-14316668 MS.gene08427:intron 40.0%
TTCAAGTATTATGGGCCACA+TGG + chr4.2:14317000-14317019 MS.gene08427:intron 40.0%
TTCCAAGAAAAGGACGACTT+TGG + chr4.2:14317250-14317269 MS.gene08427:intron 40.0%
!! ATTCGGTTAAGACGGATTAC+CGG - chr4.2:14317044-14317063 None:intergenic 40.0%
AACCTTTAGACCCATGGTCA+AGG - chr4.2:14316740-14316759 None:intergenic 45.0%
ACGAAAACTCATCGAGGAGA+AGG + chr4.2:14318004-14318023 MS.gene08427:CDS 45.0%
ACTATGTAGAGCCTTGACCA+TGG + chr4.2:14316726-14316745 MS.gene08427:intron 45.0%
AGTGATGTAGGAGAGTTCAC+TGG + chr4.2:14316180-14316199 MS.gene08427:CDS 45.0%
CTATGTAGAGCCTTGACCAT+GGG + chr4.2:14316727-14316746 MS.gene08427:intron 45.0%
CTGCTGCACCGTTAAGAAAA+AGG + chr4.2:14318048-14318067 MS.gene08427:CDS 45.0%
GACCTCAAGATATCAACCGT+GGG + chr4.2:14317107-14317126 MS.gene08427:intron 45.0%
GGTTGAACGAAAACTCATCG+AGG + chr4.2:14317998-14318017 MS.gene08427:intron 45.0%
GTACCTCTGTGGAACAGTTT+GGG - chr4.2:14316212-14316231 None:intergenic 45.0%
TAACCCAAACTGTTCCACAG+AGG + chr4.2:14316206-14316225 MS.gene08427:CDS 45.0%
TCTTAGGGCCATTCATGACA+AGG - chr4.2:14316835-14316854 None:intergenic 45.0%
TGTACCTCTGTGGAACAGTT+TGG - chr4.2:14316213-14316232 None:intergenic 45.0%
TTTGGCCAGGTTGTTCAACA+AGG + chr4.2:14317268-14317287 MS.gene08427:intron 45.0%
! CTTGGCTGGATTGAGGTTTT+GGG + chr4.2:14317130-14317149 MS.gene08427:intron 45.0%
! TGTCTCTTGTTGTGCAGCTA+CGG + chr4.2:14316490-14316509 MS.gene08427:intron 45.0%
!! ACTGTCGGTTTGTTCGGTTT+CGG - chr4.2:14317070-14317089 None:intergenic 45.0%
!! TTCGGTTAAGACGGATTACC+GGG - chr4.2:14317043-14317062 None:intergenic 45.0%
!! AAAAAAGAAAAAAAAAATAA+AGG - chr4.2:14317399-14317418 None:intergenic 5.0%
!! AAAAAATAAAAATAAAAAGT+GGG + chr4.2:14317891-14317910 MS.gene08427:intron 5.0%
AGAAAAGGACGACTTTGGCC+AGG + chr4.2:14317255-14317274 MS.gene08427:intron 50.0%
AGCCTTGACCATGGGTCTAA+AGG + chr4.2:14316735-14316754 MS.gene08427:intron 50.0%
CAAGATATCAACCGTGGGCT+TGG + chr4.2:14317112-14317131 MS.gene08427:intron 50.0%
GGACCTCAAGATATCAACCG+TGG + chr4.2:14317106-14317125 MS.gene08427:intron 50.0%
! AGCCCACGGTTGATATCTTG+AGG - chr4.2:14317112-14317131 None:intergenic 50.0%
! ATATGCCTTCGAGTTAGGGC+TGG - chr4.2:14317185-14317204 None:intergenic 50.0%
! GCTTGGCTGGATTGAGGTTT+TGG + chr4.2:14317129-14317148 MS.gene08427:intron 50.0%
! GGCCAAAGTCGTCCTTTTCT+TGG - chr4.2:14317255-14317274 None:intergenic 50.0%
! TTCGAGTTAGGGCTGGACAT+CGG - chr4.2:14317178-14317197 None:intergenic 50.0%
!! GGTTGACTCGGTTAACCATG+TGG - chr4.2:14317018-14317037 None:intergenic 50.0%
!! GTTAAGACGGATTACCGGGT+GGG - chr4.2:14317039-14317058 None:intergenic 50.0%
!! TGCAGCAGTGTCTCTGGAAT+CGG - chr4.2:14318036-14318055 None:intergenic 50.0%
AGTTAGGGCTGGACATCGGT+CGG - chr4.2:14317174-14317193 None:intergenic 55.0%
ATATCAACCGTGGGCTTGGC+TGG + chr4.2:14317116-14317135 MS.gene08427:intron 55.0%
GATGTCCAGCCCTAACTCGA+AGG + chr4.2:14317177-14317196 MS.gene08427:intron 55.0%
GTTAACCGAGTCAACCCACC+CGG + chr4.2:14317022-14317041 MS.gene08427:intron 55.0%
TAACGGTGCAGCAGTGTCTC+TGG - chr4.2:14318042-14318061 None:intergenic 55.0%
!! GATTACCGGGTGGGTTGACT+CGG - chr4.2:14317030-14317049 None:intergenic 55.0%
!! GGTTAAGACGGATTACCGGG+TGG - chr4.2:14317040-14317059 None:intergenic 55.0%
CCTCAATCCAGCCAAGCCCA+CGG - chr4.2:14317126-14317145 None:intergenic 60.0%
! GGCTGGACATCGGTCGGTTT+GGG - chr4.2:14317168-14317187 None:intergenic 60.0%
!! GGACATCGGTCGGTTTGGGT+CGG - chr4.2:14317164-14317183 None:intergenic 60.0%
!! GGTCGGTTTGGGTCGGTAGT+CGG - chr4.2:14317157-14317176 None:intergenic 60.0%
!! GTCGGTTTGGGTCGGTAGTC+GGG - chr4.2:14317156-14317175 None:intergenic 60.0%
CCGTGGGCTTGGCTGGATTG+AGG + chr4.2:14317123-14317142 MS.gene08427:intron 65.0%
GGGCTGGACATCGGTCGGTT+TGG - chr4.2:14317169-14317188 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 14316153 14318073 14316153 ID=MS.gene08427
chr4.2 mRNA 14316153 14318073 14316153 ID=MS.gene08427.t1;Parent=MS.gene08427
chr4.2 exon 14316153 14316227 14316153 ID=MS.gene08427.t1.exon1;Parent=MS.gene08427.t1
chr4.2 CDS 14316153 14316227 14316153 ID=cds.MS.gene08427.t1;Parent=MS.gene08427.t1
chr4.2 exon 14316323 14316403 14316323 ID=MS.gene08427.t1.exon2;Parent=MS.gene08427.t1
chr4.2 CDS 14316323 14316403 14316323 ID=cds.MS.gene08427.t1;Parent=MS.gene08427.t1
chr4.2 exon 14316535 14316564 14316535 ID=MS.gene08427.t1.exon3;Parent=MS.gene08427.t1
chr4.2 CDS 14316535 14316564 14316535 ID=cds.MS.gene08427.t1;Parent=MS.gene08427.t1
chr4.2 exon 14317999 14318073 14317999 ID=MS.gene08427.t1.exon4;Parent=MS.gene08427.t1
chr4.2 CDS 14317999 14318073 14317999 ID=cds.MS.gene08427.t1;Parent=MS.gene08427.t1
Gene Sequence

>MS.gene08427

ATGAATCTAGAGGCCAGCTATTGTGATAGTGATGTAGGAGAGTTCACTGGTTTTAACCCAAACTGTTCCACAGAGGTACAATAATCCCTTATTGTTTGGTAACACATTGCTTTTGATTTCAATGAAATGTGTTGGTAACACATTGCTTTTTATTTCAATTATTCAAGCAGATTATTGATTTTAATTTGGATGTGGCTACTCCTTTACTTGCTGAAATGATGGGCTGTGTAATTAATTTGAACTCAATCAAGGTTAGTTTTAGTTTTTATTTATTGAATTTAGTCTTCATTTGAACTCAATCTATAAAAAAATACAAATTTTATATCTGATGTTACATTGTCTCTTGTTGTGCAGCTACGGTATTTGTGTGACCATGTTGTAGCATCAGCAATTGCAAGCATCTCATGCCGATGTAAATTATGTAGAATTCTAGATGACTGTAATTACGGCATTGTGAAGTTTATATTGCTGGTTGTGTTGTGCTAGTCTATGTCTTGCCTAGTGCAATGACAATTATGGTTATGATTTCTATTTGTAAACTACTAAACTTAATTATGCCAGTATGCAGTATGCACTATGTAGAGCCTTGACCATGGGTCTAAAGGTTTCTTATTTTATGGTTATGATTTCTTCAATTCTATTGAATGGCCTAATGAAAAATGGGCATAGGTTTAAAAAACCTTGTCATGAATGGCCCTAAGATTATATTTACTTAATGACTGAATTTTATATTAAAATGGACTCTCAAGTTTGTTTATAGGGAAAAATCATGGTTATGGTTTCTTAAAATATCAGCAGGTCTAAATCAAAATTGTAATTTTTTTTTTTATAAAAATAATTATGTAATTTCAAGTATTATGGGCCACATGGTTAACCGAGTCAACCCACCCGGTAATCCGTCTTAACCGAATAAACCGAAACCGAACAAACCGACAGTTAAAACAGTCGGAATTGGACCTCAAGATATCAACCGTGGGCTTGGCTGGATTGAGGTTTTGGGCCCGACTACCGACCCAAACCGACCGATGTCCAGCCCTAACTCGAAGGCATATTGTATGGATGACTTATTTGGAAAGAGAGAAATTCTGGTTTTTTTTTTCCAAGAAAAGGACGACTTTGGCCAGGTTGTTCAACAAGGTTAAATTACTATTGTTTTAGTGGATTAAAGCCAAAAATCTTGCTTTTATTTGTGATATTAATCAATGATGGACAATTCTTTTATTTTATATGGGGTGATCTAGCACCTTTATTTTTTTTTTCTTTTTTTCTTCCAGTTTTGTTACCTTTCGTGTGTTTGCATCATTTCGTTGACTCTAAGAGCTCTCCTCGTGTAAGTTTTATGCTAAGAAGGCCTTTTGGTTTATTAGCACCTTTATTTTGGTTTGTTCAAAAAATGTGTTAAATTAAAAATAAGGATGCCAAAATCTAAAAATTAACAAAATAGGAGGACAAAAATTGCAATCAAACCTAAGATTTAAGTAAAATAAGTGATATTTTTTATACTTTATGAAATAAATAAACAAATTTCATAAATTTTAACAAATTGTTCATTATCCCCCTAAGAAACAAATTATTGTTTTTTTATATACATTTTTTAAGCAATTTTTTTTATTTTTATATACTTTTTTTGAAGAATATTTTTATATACTTTTTTTAAGAAGAATATTTTTATATACTTAATGAGCTGAATTATTAGAGAAAATTTGTTATTATTAAAAAAAAGTAAAACGAGAAGAGGAAAAAATAAAAATAAAAAGTGGGAAAATAATCTTAATAATTTTATCCCTCAATTTCATTTTATTTATTAAAAAAAATCATTTTATCATTTTATGTAGCTTTTGTCTAGGTTGAACGAAAACTCATCGAGGAGAAGGCGAAATCCGATTCCAGAGACACTGCTGCACCGTTAAGAAAAAGGTAA

Protein sequence

>MS.gene08427.t1

MNLEASYCDSDVGEFTGFNPNCSTEIIDFNLDVATPLLAEMMGCVINLNSIKHQQLQASHADVERKLIEEKAKSDSRDTAAPLRKR