AlfalfaGEDB | MS.gene97914

M. sativa cultivar XinJiangDaYe / MS.gene97914

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016787
PFAM: PF07859.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97914.t1	XP_013452514.1	92.3	325	25	0	1	325	6	330	3.90E-175	624

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97914.t1	Q0ZPV7	52.8	318	144	2	5	320	14	327	2.8e-89	330.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97914.t1	A0A072UA74	92.3	325	25	0	1	325	6	330	2.8e-175	624.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049222	MS.gene97914	0.8152	1.04E-51	-1.69E-46
MS.gene049232	MS.gene97914	0.802682	5.00E-49	-1.69E-46
MS.gene049609	MS.gene97914	0.821085	4.87E-53	-1.69E-46
MS.gene049805	MS.gene97914	0.831566	1.55E-55	-1.69E-46
MS.gene05010	MS.gene97914	0.81129	7.54E-51	-1.69E-46
MS.gene050170	MS.gene97914	0.803803	2.93E-49	-1.69E-46
MS.gene050323	MS.gene97914	0.81048	1.13E-50	-1.69E-46
MS.gene050496	MS.gene97914	0.808575	2.90E-50	-1.69E-46
MS.gene050502	MS.gene97914	0.811705	6.13E-51	-1.69E-46
MS.gene050504	MS.gene97914	0.808195	3.49E-50	-1.69E-46
MS.gene050520	MS.gene97914	0.810378	1.19E-50	-1.69E-46
MS.gene050612	MS.gene97914	0.803647	3.15E-49	-1.69E-46
MS.gene050675	MS.gene97914	0.812206	4.76E-51	-1.69E-46
MS.gene050699	MS.gene97914	0.810782	9.71E-51	-1.69E-46
MS.gene050747	MS.gene97914	0.822483	2.31E-53	-1.69E-46
MS.gene050772	MS.gene97914	0.822892	1.86E-53	-1.69E-46
MS.gene050868	MS.gene97914	0.830913	2.25E-55	-1.69E-46
MS.gene050871	MS.gene97914	0.834419	3.03E-56	-1.69E-46
MS.gene050950	MS.gene97914	0.817017	4.10E-52	-1.69E-46
MS.gene050986	MS.gene97914	0.805813	1.11E-49	-1.69E-46
MS.gene051167	MS.gene97914	-0.812609	3.89E-51	-1.69E-46
MS.gene051387	MS.gene97914	0.804491	2.11E-49	-1.69E-46
MS.gene051509	MS.gene97914	0.81118	7.96E-51	-1.69E-46
MS.gene051941	MS.gene97914	0.801415	9.10E-49	-1.69E-46
MS.gene052318	MS.gene97914	0.810046	1.40E-50	-1.69E-46
MS.gene052421	MS.gene97914	0.802105	6.57E-49	-1.69E-46
MS.gene052503	MS.gene97914	0.807018	6.20E-50	-1.69E-46
MS.gene052504	MS.gene97914	0.806178	9.33E-50	-1.69E-46
MS.gene052506	MS.gene97914	0.801097	1.06E-48	-1.69E-46
MS.gene052615	MS.gene97914	0.850597	1.53E-60	-1.69E-46
MS.gene052823	MS.gene97914	0.807529	4.84E-50	-1.69E-46
MS.gene052825	MS.gene97914	0.807643	4.57E-50	-1.69E-46
MS.gene052966	MS.gene97914	0.800621	1.32E-48	-1.69E-46
MS.gene053203	MS.gene97914	0.812454	4.21E-51	-1.69E-46
MS.gene053207	MS.gene97914	0.814364	1.60E-51	-1.69E-46
MS.gene053213	MS.gene97914	0.836112	1.13E-56	-1.69E-46
MS.gene053286	MS.gene97914	0.834739	2.52E-56	-1.69E-46
MS.gene053436	MS.gene97914	0.819552	1.09E-52	-1.69E-46
MS.gene053480	MS.gene97914	0.809373	1.95E-50	-1.69E-46
MS.gene053505	MS.gene97914	0.8122	4.78E-51	-1.69E-46
MS.gene053574	MS.gene97914	0.82003	8.51E-53	-1.69E-46
MS.gene053626	MS.gene97914	0.839035	2.02E-57	-1.69E-46
MS.gene053632	MS.gene97914	0.812017	5.24E-51	-1.69E-46
MS.gene053813	MS.gene97914	0.810669	1.03E-50	-1.69E-46
MS.gene053824	MS.gene97914	0.823864	1.10E-53	-1.69E-46
MS.gene054408	MS.gene97914	0.814263	1.68E-51	-1.69E-46
MS.gene054486	MS.gene97914	0.824075	9.82E-54	-1.69E-46
MS.gene054595	MS.gene97914	0.836312	1.01E-56	-1.69E-46
MS.gene05494	MS.gene97914	0.815491	9.00E-52	-1.69E-46
MS.gene05505	MS.gene97914	0.825921	3.60E-54	-1.69E-46
MS.gene055362	MS.gene97914	0.806302	8.79E-50	-1.69E-46
MS.gene055577	MS.gene97914	0.83129	1.82E-55	-1.69E-46
MS.gene055909	MS.gene97914	0.835795	1.36E-56	-1.69E-46
MS.gene055911	MS.gene97914	0.84004	1.10E-57	-1.69E-46
MS.gene055941	MS.gene97914	0.824182	9.27E-54	-1.69E-46
MS.gene05601	MS.gene97914	0.807699	4.45E-50	-1.69E-46
MS.gene056448	MS.gene97914	0.800128	1.67E-48	-1.69E-46
MS.gene056504	MS.gene97914	0.822639	2.13E-53	-1.69E-46
MS.gene056510	MS.gene97914	0.814249	1.70E-51	-1.69E-46
MS.gene056657	MS.gene97914	0.808924	2.44E-50	-1.69E-46
MS.gene05666	MS.gene97914	0.813455	2.54E-51	-1.69E-46
MS.gene057076	MS.gene97914	0.810752	9.86E-51	-1.69E-46
MS.gene057086	MS.gene97914	0.819075	1.41E-52	-1.69E-46
MS.gene057416	MS.gene97914	0.809081	2.26E-50	-1.69E-46
MS.gene057856	MS.gene97914	0.807534	4.82E-50	-1.69E-46
MS.gene057857	MS.gene97914	0.800945	1.14E-48	-1.69E-46
MS.gene05787	MS.gene97914	0.814402	1.57E-51	-1.69E-46
MS.gene058130	MS.gene97914	0.806208	9.20E-50	-1.69E-46
MS.gene058157	MS.gene97914	0.83824	3.23E-57	-1.69E-46
MS.gene05837	MS.gene97914	0.817882	2.62E-52	-1.69E-46
MS.gene059367	MS.gene97914	0.843414	1.42E-58	-1.69E-46
MS.gene059368	MS.gene97914	0.836587	8.57E-57	-1.69E-46
MS.gene059674	MS.gene97914	0.813565	2.40E-51	-1.69E-46
MS.gene059718	MS.gene97914	0.820297	7.39E-53	-1.69E-46
MS.gene059865	MS.gene97914	0.806345	8.60E-50	-1.69E-46
MS.gene060372	MS.gene97914	0.803996	2.67E-49	-1.69E-46
MS.gene060482	MS.gene97914	0.82072	5.91E-53	-1.69E-46
MS.gene060778	MS.gene97914	0.852678	3.94E-61	-1.69E-46
MS.gene06077	MS.gene97914	0.815501	8.95E-52	-1.69E-46
MS.gene060835	MS.gene97914	0.813104	3.03E-51	-1.69E-46
MS.gene060837	MS.gene97914	0.809591	1.75E-50	-1.69E-46
MS.gene060982	MS.gene97914	0.809402	1.93E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene87835	MS.gene97914	PPI
MS.gene97914	MS.gene37505	PPI
MS.gene23638	MS.gene97914	PPI
MS.gene97914	MS.gene019398	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97914.t1	MTR_6g464320	92.308	325	25	0	1	325	6	330	0.0	630
MS.gene97914.t1	MTR_6g464280	84.326	319	50	0	3	321	7	325	0.0	554
MS.gene97914.t1	MTR_6g464510	69.782	321	91	4	3	321	11	327	2.48e-158	446
MS.gene97914.t1	MTR_1g102160	53.870	323	138	4	4	320	8	325	6.84e-114	333
MS.gene97914.t1	MTR_1g102170	52.215	316	142	3	6	319	29	337	5.97e-113	331
MS.gene97914.t1	MTR_6g464120	54.019	311	137	5	3	312	12	317	3.68e-106	320
MS.gene97914.t1	MTR_0007s0520	51.316	304	138	5	22	321	28	325	5.54e-99	295
MS.gene97914.t1	MTR_8g035520	47.720	329	156	5	3	324	9	328	2.30e-95	286
MS.gene97914.t1	MTR_8g035540	46.296	324	154	6	8	320	2	316	3.04e-89	270
MS.gene97914.t1	MTR_8g035500	36.562	320	189	6	8	320	5	317	4.64e-73	228
MS.gene97914.t1	MTR_1g111280	35.629	334	175	9	12	325	33	346	1.19e-54	182
MS.gene97914.t1	MTR_0007s0480	54.651	172	72	4	154	321	2	171	1.70e-52	171
MS.gene97914.t1	MTR_7g406880	34.505	313	178	9	30	321	44	350	3.44e-52	176
MS.gene97914.t1	MTR_6g065430	38.306	248	143	4	42	289	58	295	5.94e-48	164
MS.gene97914.t1	MTR_6g065120	38.559	236	131	5	20	244	33	265	1.36e-46	160
MS.gene97914.t1	MTR_6g065110	34.333	300	185	6	27	321	43	335	1.80e-45	158
MS.gene97914.t1	MTR_6g065400	33.546	313	187	8	20	321	32	334	5.10e-45	157
MS.gene97914.t1	MTR_6g065270	36.667	240	138	4	12	240	22	258	2.40e-44	155
MS.gene97914.t1	MTR_7g107040	37.838	259	136	9	76	320	69	316	1.28e-43	152
MS.gene97914.t1	MTR_6g065370	32.907	313	189	8	20	321	32	334	2.04e-43	152
MS.gene97914.t1	MTR_4g050140	30.031	323	195	6	15	323	13	318	1.53e-42	149
MS.gene97914.t1	MTR_1g012430	32.836	268	167	5	20	286	15	270	1.39e-41	147
MS.gene97914.t1	MTR_7g056207	33.220	295	176	9	34	321	67	347	3.88e-41	147
MS.gene97914.t1	MTR_4g086510	31.879	298	179	9	34	321	35	318	1.84e-39	141
MS.gene97914.t1	MTR_4g086480	32.215	298	173	10	34	320	39	318	1.61e-36	134
MS.gene97914.t1	MTR_6g065340	34.272	213	117	4	32	240	58	251	1.79e-35	131
MS.gene97914.t1	MTR_3g101420	31.230	317	186	9	18	321	18	315	1.93e-35	130
MS.gene97914.t1	MTR_1g089310	36.638	232	135	5	74	304	119	339	2.22e-35	132
MS.gene97914.t1	MTR_1g089310	36.638	232	135	5	74	304	103	323	2.22e-35	131
MS.gene97914.t1	MTR_7g093950	32.753	287	173	7	48	321	68	347	3.13e-35	131
MS.gene97914.t1	MTR_4g086470	32.171	258	154	7	34	286	38	279	1.67e-34	128
MS.gene97914.t1	MTR_1g082210	30.592	304	183	9	10	300	31	319	3.75e-31	120
MS.gene97914.t1	MTR_4g086540	28.571	301	181	7	23	321	34	302	4.16e-30	116
MS.gene97914.t1	MTR_1g105020	30.745	322	196	12	12	320	15	322	1.14e-29	115
MS.gene97914.t1	MTR_3g012440	30.802	237	149	5	58	289	74	300	1.94e-28	112
MS.gene97914.t1	MTR_3g450680	29.618	314	193	11	22	320	31	331	2.49e-28	112
MS.gene97914.t1	MTR_8g040860	29.022	317	192	13	20	320	29	328	3.39e-27	109
MS.gene97914.t1	MTR_5g005900	27.217	327	200	13	15	320	17	326	4.83e-26	105
MS.gene97914.t1	MTR_5g005850	28.528	326	195	12	15	320	21	328	1.74e-25	104
MS.gene97914.t1	MTR_5g005920	26.129	310	190	11	28	320	43	330	8.79e-25	102
MS.gene97914.t1	MTR_6g022710	31.250	208	109	3	70	243	154	361	6.81e-23	99.0
MS.gene97914.t1	MTR_7g051020	29.747	316	188	12	22	320	27	325	2.06e-22	95.9
MS.gene97914.t1	MTR_1g104930	30.830	253	149	8	44	286	48	284	4.76e-21	92.0
MS.gene97914.t1	MTR_1g104933	31.765	255	140	10	44	286	46	278	2.52e-18	84.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97914.t1	AT5G62180	46.729	321	167	3	3	320	4	323	3.25e-102	303
MS.gene97914.t1	AT2G45600	43.769	329	161	7	3	322	4	317	1.10e-88	269
MS.gene97914.t1	AT2G45610	39.939	328	178	6	4	321	6	324	2.00e-78	242
MS.gene97914.t1	AT5G16080	36.431	269	148	7	30	289	51	305	2.19e-48	166
MS.gene97914.t1	AT1G68620	36.667	300	169	8	1	297	19	300	6.12e-47	161
MS.gene97914.t1	AT5G23530	36.842	228	130	4	17	240	32	249	5.34e-41	146
MS.gene97914.t1	AT5G06570	35.354	297	162	10	42	323	47	328	4.63e-38	138
MS.gene97914.t1	AT5G06570	35.354	297	162	10	42	323	47	328	4.63e-38	138
MS.gene97914.t1	AT5G06570	35.354	297	162	10	42	323	62	343	5.56e-38	138
MS.gene97914.t1	AT5G06570	35.354	297	162	10	42	323	62	343	5.56e-38	138
MS.gene97914.t1	AT1G19190	31.250	272	168	7	20	286	18	275	1.48e-35	131
MS.gene97914.t1	AT1G47480	28.025	314	202	8	18	325	19	314	4.51e-34	127
MS.gene97914.t1	AT1G49640	34.197	193	123	3	7	199	6	194	5.50e-34	127
MS.gene97914.t1	AT3G48700	32.000	250	156	5	34	275	32	275	3.03e-33	125
MS.gene97914.t1	AT5G27320	34.940	249	131	8	10	242	31	264	3.65e-33	125
MS.gene97914.t1	AT3G63010	34.156	243	139	6	49	289	86	309	7.15e-32	122
MS.gene97914.t1	AT3G48690	29.123	285	178	8	12	286	12	282	8.28e-31	119
MS.gene97914.t1	AT3G05120	39.053	169	95	3	75	242	105	266	1.81e-27	110
MS.gene97914.t1	AT1G49660	33.140	172	106	3	35	201	33	200	7.83e-27	108
MS.gene97914.t1	AT5G14310	27.444	266	150	3	16	243	96	356	3.95e-25	105
MS.gene97914.t1	AT1G49650	32.353	170	105	4	35	199	89	253	8.18e-25	103
MS.gene97914.t1	AT2G03550	30.939	181	110	4	23	197	21	192	1.77e-23	99.0
MS.gene97914.t1	AT3G27320	30.137	219	111	4	65	243	123	339	2.96e-23	99.8
MS.gene97914.t1	AT3G27320	30.137	219	111	4	65	243	155	371	3.68e-23	99.8

Find 84 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACACATAATTCATGAAATT+TGG	0.180622	6.1:+53272890	None:intergenic
GTGGCAGTAACTTGTTATTT+TGG	0.184949	6.1:+53272964	None:intergenic
ATAAATTTCTTTGCTTTAGA+TGG	0.233057	6.1:+53272258	None:intergenic
TTGGTTGATGTTAAGATCTT+TGG	0.327803	6.1:+53273043	None:intergenic
CTATTCTAAGTGTTACCTAA+TGG	0.334421	6.1:-53272718	MS.gene97914:CDS
TCATGAATTATGTGTTGATA+TGG	0.358457	6.1:-53272883	MS.gene97914:CDS
TAAGGGTGTGGATGTTGTTA+AGG	0.362095	6.1:-53272322	MS.gene97914:CDS
TGGAAGCATTGAGTTTGATT+AGG	0.366794	6.1:-53272776	MS.gene97914:CDS
TATTCTAAGTGTTACCTAAT+GGG	0.394316	6.1:-53272717	MS.gene97914:CDS
TTAAGATCTTTGGTAAGAAC+AGG	0.400333	6.1:+53273053	None:intergenic
TGGGAGTTGGCATTACCTAT+TGG	0.407630	6.1:-53272504	MS.gene97914:CDS
GGAATAATCGAAGCCATGTT+TGG	0.417087	6.1:+53273015	None:intergenic
CATCATATGCCGCCGGTAAC+CGG	0.417101	6.1:+53272805	None:intergenic
CGCACCGGAGCACCGGTTAC+CGG	0.420881	6.1:-53272817	MS.gene97914:CDS
ATATCGAGTAAGTGTATCAT+TGG	0.426484	6.1:+53273118	None:intergenic
CAACTCTGATTCAGTCCTAT+TGG	0.435907	6.1:+53272575	None:intergenic
GTGTGTTAGTGATTTGTGTT+GGG	0.440638	6.1:-53272523	MS.gene97914:CDS
TGATCACGATCAACTCCAAT+AGG	0.440937	6.1:+53272489	None:intergenic
GGATCAAGGGTGGAGGCTAT+TGG	0.445047	6.1:-53272409	MS.gene97914:CDS
CTCCAGCACTATTACCCATT+AGG	0.447172	6.1:+53272703	None:intergenic
TAAGGATCGGTTGTCGGTGG+TGG	0.465102	6.1:+53273167	None:intergenic
GAAGCCATGTTTGGTTTGAT+TGG	0.466485	6.1:+53273024	None:intergenic
TGTCAACGGTGGGGATCTAT+TGG	0.468178	6.1:-53272382	MS.gene97914:CDS
GCCGCCGGTAACCGGTGCTC+CGG	0.468450	6.1:+53272813	None:intergenic
CTAACACACAAAGGAAAATC+CGG	0.478583	6.1:+53272537	None:intergenic
TGTGTGTTAGTGATTTGTGT+TGG	0.479150	6.1:-53272524	MS.gene97914:CDS
TAGTGATTTGTGTTGGGAGT+TGG	0.482769	6.1:-53272517	MS.gene97914:CDS
TCAACATCAATCTCAACCAT+TGG	0.489151	6.1:+53272453	None:intergenic
TACCTAATGGGTAATAGTGC+TGG	0.489408	6.1:-53272705	MS.gene97914:CDS
GCTACCATTGCATACCATGC+AGG	0.493871	6.1:-53272681	MS.gene97914:CDS
TTAGATAAGATAAAGGATCA+AGG	0.495282	6.1:-53272423	MS.gene97914:CDS
GTTGAGACTTGAGAATGATC+CGG	0.497765	6.1:-53272556	MS.gene97914:CDS
ACATAATTCATGAAATTTGG+TGG	0.500259	6.1:+53272893	None:intergenic
TTTGAGGAGTTGGTAAGGAT+CGG	0.503990	6.1:+53273154	None:intergenic
GTGTATCATTGGGATGGTGG+TGG	0.506884	6.1:+53273129	None:intergenic
AAAAGAGTTGGTGCAATTGA+TGG	0.507179	6.1:-53272349	MS.gene97914:CDS
ATCAACCCTTGGATCTTCAA+TGG	0.512904	6.1:+53272624	None:intergenic
GGTGCAATTGATGGAGGATA+AGG	0.517686	6.1:-53272340	MS.gene97914:CDS
TTTCAAGAAGATGGTTGCCA+TGG	0.523109	6.1:-53272297	MS.gene97914:CDS
AGTTGGTAAGGATCGGTTGT+CGG	0.523290	6.1:+53273161	None:intergenic
GAAATTTATTGAGTTAGTGA+AGG	0.523975	6.1:-53272244	MS.gene97914:CDS
CCATGAAAGAAAACAATAAG+TGG	0.524990	6.1:+53272945	None:intergenic
TGTCGAAGCAGTTGTCGCCT+CGG	0.526890	6.1:-53272853	MS.gene97914:CDS
TATCGAGTAAGTGTATCATT+GGG	0.528344	6.1:+53273119	None:intergenic
TTAGGTCAAGCAAAGATGAA+TGG	0.530318	6.1:-53272758	MS.gene97914:CDS
ATCGCCTCGCACCGGAGCAC+CGG	0.536876	6.1:-53272824	MS.gene97914:CDS
TTTGAGCCATTGAAGATCCA+AGG	0.542044	6.1:-53272630	MS.gene97914:CDS
AAATTTATTGAGTTAGTGAA+GGG	0.543509	6.1:-53272243	MS.gene97914:CDS
GGAAGAGAAGTGTCTAATGA+AGG	0.545290	6.1:+53273074	None:intergenic
GGTTGTCGGTGGTGGATGCA+TGG	0.545849	6.1:+53273175	None:intergenic
TAGATAAGATAAAGGATCAA+GGG	0.557087	6.1:-53272422	MS.gene97914:CDS
TATTGGTGAGTGTCAACGGT+GGG	0.568537	6.1:-53272392	MS.gene97914:CDS
GGCGGCATATGATGATGCTA+TGG	0.579015	6.1:-53272796	MS.gene97914:CDS
TTGAGCCATTGAAGATCCAA+GGG	0.580380	6.1:-53272629	MS.gene97914:CDS
GTAACCGGTGCTCCGGTGCG+AGG	0.582296	6.1:+53272820	None:intergenic
AGAAGTGTCTAATGAAGGTG+AGG	0.582650	6.1:+53273079	None:intergenic
TAATTCATGAAATTTGGTGG+AGG	0.583966	6.1:+53272896	None:intergenic
ATTGATGGAGGATAAGGGTG+TGG	0.586811	6.1:-53272334	MS.gene97914:CDS
ATAAGATAAAGGATCAAGGG+TGG	0.587545	6.1:-53272419	MS.gene97914:CDS
GTGCAATTGATGGAGGATAA+GGG	0.588351	6.1:-53272339	MS.gene97914:CDS
GTTGACGCAATATCAACCCT+TGG	0.594787	6.1:+53272613	None:intergenic
CAACTACACGTAGCCCTGCA+TGG	0.595917	6.1:+53272667	None:intergenic
AGATAAAGGATCAAGGGTGG+AGG	0.597761	6.1:-53272416	MS.gene97914:CDS
AGGCTATTGGTGAGTGTCAA+CGG	0.603086	6.1:-53272396	MS.gene97914:CDS
CGAAGCAGTTGTCGCCTCGG+TGG	0.606345	6.1:-53272850	MS.gene97914:CDS
GGTGGATTATCGCCTCGCAC+CGG	0.611850	6.1:-53272832	MS.gene97914:CDS
GGTTGATCGTGCAAAAGAGT+TGG	0.615439	6.1:-53272361	MS.gene97914:CDS
TCAACCAATCAAACCAAACA+TGG	0.620670	6.1:-53273028	MS.gene97914:CDS
CACAAATCACTAACACACAA+AGG	0.622904	6.1:+53272528	None:intergenic
CTACCATTGCATACCATGCA+GGG	0.623078	6.1:-53272680	MS.gene97914:CDS
GAGTAAGTGTATCATTGGGA+TGG	0.623555	6.1:+53273123	None:intergenic
ATTGGTGAGTGTCAACGGTG+GGG	0.628502	6.1:-53272391	MS.gene97914:CDS
TAGCCCTGCATGGTATGCAA+TGG	0.633352	6.1:+53272677	None:intergenic
AGAGTTGGTGCAATTGATGG+AGG	0.635224	6.1:-53272346	MS.gene97914:CDS
TGGTAAGGATCGGTTGTCGG+TGG	0.635695	6.1:+53273164	None:intergenic
ATAGCATCATCATATGCCGC+CGG	0.638454	6.1:+53272798	None:intergenic
ACCGGAGCACCGGTTACCGG+CGG	0.650002	6.1:-53272814	MS.gene97914:CDS
TGAATTATGTGTTGATATGG+CGG	0.656830	6.1:-53272880	MS.gene97914:CDS
TAAGTGTATCATTGGGATGG+TGG	0.658508	6.1:+53273126	None:intergenic
TCATGAGTATTGCAATCCAA+TGG	0.662746	6.1:-53272469	MS.gene97914:CDS
TCTAATGAAGGTGAGGTGTG+TGG	0.664418	6.1:+53273086	None:intergenic
GTTTAATTAAGCATCGAACG+TGG	0.668258	6.1:+53272212	None:intergenic
CTATTGGTGAGTGTCAACGG+TGG	0.715797	6.1:-53272393	MS.gene97914:CDS
TGCGAGGCGATAATCCACCG+AGG	0.794949	6.1:+53272836	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGAAAAATTAGATAAGATAA+AGG	-	chr6.1:53272961-53272980	MS.gene97914:CDS	15.0%
!!	AAATTTATTGAGTTAGTGAA+GGG	-	chr6.1:53273148-53273167	MS.gene97914:CDS	20.0%
!!	AACACATAATTCATGAAATT+TGG	+	chr6.1:53272504-53272523	None:intergenic	20.0%
!!!	ATAAATTTCTTTGCTTTAGA+TGG	+	chr6.1:53273136-53273155	None:intergenic	20.0%
!!!	GAAATATTTGTTGCTTTTTT+AGG	+	chr6.1:53272404-53272423	None:intergenic	20.0%
!	ACATAATTCATGAAATTTGG+TGG	+	chr6.1:53272501-53272520	None:intergenic	25.0%
!	GAAATTTATTGAGTTAGTGA+AGG	-	chr6.1:53273147-53273166	MS.gene97914:CDS	25.0%
!	TAGATAAGATAAAGGATCAA+GGG	-	chr6.1:53272969-53272988	MS.gene97914:CDS	25.0%
!	TATTCTAAGTGTTACCTAAT+GGG	-	chr6.1:53272674-53272693	MS.gene97914:CDS	25.0%
!	TTAGATAAGATAAAGGATCA+AGG	-	chr6.1:53272968-53272987	MS.gene97914:CDS	25.0%
!!	TCATGAATTATGTGTTGATA+TGG	-	chr6.1:53272508-53272527	MS.gene97914:CDS	25.0%
!!!	TATTTGTTGCTTTTTTAGGT+AGG	+	chr6.1:53272400-53272419	None:intergenic	25.0%
	ATATCGAGTAAGTGTATCAT+TGG	+	chr6.1:53272276-53272295	None:intergenic	30.0%
	CCATGAAAGAAAACAATAAG+TGG	+	chr6.1:53272449-53272468	None:intergenic	30.0%
	CTATTCTAAGTGTTACCTAA+TGG	-	chr6.1:53272673-53272692	MS.gene97914:CDS	30.0%
	TATCGAGTAAGTGTATCATT+GGG	+	chr6.1:53272275-53272294	None:intergenic	30.0%
	TTAAGATCTTTGGTAAGAAC+AGG	+	chr6.1:53272341-53272360	None:intergenic	30.0%
!	ATTGTTTTCTTTCATGGTAG+TGG	-	chr6.1:53272452-53272471	MS.gene97914:CDS	30.0%
!	CCACTTATTGTTTTCTTTCA+TGG	-	chr6.1:53272446-53272465	MS.gene97914:CDS	30.0%
!	TAATTCATGAAATTTGGTGG+AGG	+	chr6.1:53272498-53272517	None:intergenic	30.0%
!	TGAATTATGTGTTGATATGG+CGG	-	chr6.1:53272511-53272530	MS.gene97914:CDS	30.0%
!	TTGGTTGATGTTAAGATCTT+TGG	+	chr6.1:53272351-53272370	None:intergenic	30.0%
!!	GTTAAGGATTTTCAAGAAGA+TGG	-	chr6.1:53273085-53273104	MS.gene97914:CDS	30.0%
!!!	ATATTGCGTCAACCATTTTT+TGG	-	chr6.1:53272788-53272807	MS.gene97914:CDS	30.0%
	ATAAGATAAAGGATCAAGGG+TGG	-	chr6.1:53272972-53272991	MS.gene97914:CDS	35.0%
	CACAAATCACTAACACACAA+AGG	+	chr6.1:53272866-53272885	None:intergenic	35.0%
	CTAACACACAAAGGAAAATC+CGG	+	chr6.1:53272857-53272876	None:intergenic	35.0%
	TCAACATCAATCTCAACCAT+TGG	+	chr6.1:53272941-53272960	None:intergenic	35.0%
	TCAACCAATCAAACCAAACA+TGG	-	chr6.1:53272363-53272382	MS.gene97914:CDS	35.0%
	TTAGGTCAAGCAAAGATGAA+TGG	-	chr6.1:53272633-53272652	MS.gene97914:CDS	35.0%
!	GTGTGTTAGTGATTTGTGTT+GGG	-	chr6.1:53272868-53272887	MS.gene97914:CDS	35.0%
!	TCATGAGTATTGCAATCCAA+TGG	-	chr6.1:53272922-53272941	MS.gene97914:CDS	35.0%
!	TGTGTGTTAGTGATTTGTGT+TGG	-	chr6.1:53272867-53272886	MS.gene97914:CDS	35.0%
!!	AAAAGAGTTGGTGCAATTGA+TGG	-	chr6.1:53273042-53273061	MS.gene97914:CDS	35.0%
!!	TGGAAGCATTGAGTTTGATT+AGG	-	chr6.1:53272615-53272634	MS.gene97914:CDS	35.0%
!!!	GTGGCAGTAACTTGTTATTT+TGG	+	chr6.1:53272430-53272449	None:intergenic	35.0%
	ATCAACCCTTGGATCTTCAA+TGG	+	chr6.1:53272770-53272789	None:intergenic	40.0%
	CAACTCTGATTCAGTCCTAT+TGG	+	chr6.1:53272819-53272838	None:intergenic	40.0%
	CTATTGGTCCCACCAAAAAA+TGG	+	chr6.1:53272803-53272822	None:intergenic	40.0%
	GAGTAAGTGTATCATTGGGA+TGG	+	chr6.1:53272271-53272290	None:intergenic	40.0%
	GCTCAAAAAACTCAACTCCA+TGG	+	chr6.1:53273114-53273133	None:intergenic	40.0%
	GGAATAATCGAAGCCATGTT+TGG	+	chr6.1:53272379-53272398	None:intergenic	40.0%
	GTGCAATTGATGGAGGATAA+GGG	-	chr6.1:53273052-53273071	MS.gene97914:CDS	40.0%
	GTTGAGACTTGAGAATGATC+CGG	-	chr6.1:53272835-53272854	MS.gene97914:CDS	40.0%
	TAAGTGTATCATTGGGATGG+TGG	+	chr6.1:53272268-53272287	None:intergenic	40.0%
	TGATCACGATCAACTCCAAT+AGG	+	chr6.1:53272905-53272924	None:intergenic	40.0%
	TTGAGCCATTGAAGATCCAA+GGG	-	chr6.1:53272762-53272781	MS.gene97914:CDS	40.0%
	TTTCAAGAAGATGGTTGCCA+TGG	-	chr6.1:53273094-53273113	MS.gene97914:CDS	40.0%
	TTTGAGCCATTGAAGATCCA+AGG	-	chr6.1:53272761-53272780	MS.gene97914:CDS	40.0%
!	TAAGGGTGTGGATGTTGTTA+AGG	-	chr6.1:53273069-53273088	MS.gene97914:CDS	40.0%
!	TACCTAATGGGTAATAGTGC+TGG	-	chr6.1:53272686-53272705	MS.gene97914:CDS	40.0%
!	TAGTGATTTGTGTTGGGAGT+TGG	-	chr6.1:53272874-53272893	MS.gene97914:CDS	40.0%
!!	AGAAGTGTCTAATGAAGGTG+AGG	+	chr6.1:53272315-53272334	None:intergenic	40.0%
!!	GAAGCCATGTTTGGTTTGAT+TGG	+	chr6.1:53272370-53272389	None:intergenic	40.0%
!!	GGAAGAGAAGTGTCTAATGA+AGG	+	chr6.1:53272320-53272339	None:intergenic	40.0%
!!	TTTGAGGAGTTGGTAAGGAT+CGG	+	chr6.1:53272240-53272259	None:intergenic	40.0%
!!!	CATTTTTTGGTGGGACCAAT+AGG	-	chr6.1:53272801-53272820	MS.gene97914:CDS	40.0%
!!!	TGCGTCAACCATTTTTTGGT+GGG	-	chr6.1:53272792-53272811	MS.gene97914:CDS	40.0%
!!!	TGGATTTTGAGGAGTTGGTA+AGG	+	chr6.1:53272245-53272264	None:intergenic	40.0%
!!!	TTGCGTCAACCATTTTTTGG+TGG	-	chr6.1:53272791-53272810	MS.gene97914:CDS	40.0%
	AGATAAAGGATCAAGGGTGG+AGG	-	chr6.1:53272975-53272994	MS.gene97914:CDS	45.0%
	AGGCTATTGGTGAGTGTCAA+CGG	-	chr6.1:53272995-53273014	MS.gene97914:CDS	45.0%
	ATAGCATCATCATATGCCGC+CGG	+	chr6.1:53272596-53272615	None:intergenic	45.0%
	ATTGATGGAGGATAAGGGTG+TGG	-	chr6.1:53273057-53273076	MS.gene97914:CDS	45.0%
	CTACCATTGCATACCATGCA+GGG	-	chr6.1:53272711-53272730	MS.gene97914:CDS	45.0%
	CTCCAGCACTATTACCCATT+AGG	+	chr6.1:53272691-53272710	None:intergenic	45.0%
	GGTGCAATTGATGGAGGATA+AGG	-	chr6.1:53273051-53273070	MS.gene97914:CDS	45.0%
	GGTTGATCGTGCAAAAGAGT+TGG	-	chr6.1:53273030-53273049	MS.gene97914:CDS	45.0%
	GTTGACGCAATATCAACCCT+TGG	+	chr6.1:53272781-53272800	None:intergenic	45.0%
	TATTGGTGAGTGTCAACGGT+GGG	-	chr6.1:53272999-53273018	MS.gene97914:CDS	45.0%
	TCTAATGAAGGTGAGGTGTG+TGG	+	chr6.1:53272308-53272327	None:intergenic	45.0%
	TGGGAGTTGGCATTACCTAT+TGG	-	chr6.1:53272887-53272906	MS.gene97914:CDS	45.0%
!!	AGAGTTGGTGCAATTGATGG+AGG	-	chr6.1:53273045-53273064	MS.gene97914:CDS	45.0%
!!	AGTTGGTAAGGATCGGTTGT+CGG	+	chr6.1:53272233-53272252	None:intergenic	45.0%
	ATTGGTGAGTGTCAACGGTG+GGG	-	chr6.1:53273000-53273019	MS.gene97914:CDS	50.0%
	CTATTGGTGAGTGTCAACGG+TGG	-	chr6.1:53272998-53273017	MS.gene97914:CDS	50.0%
	GCTACCATTGCATACCATGC+AGG	-	chr6.1:53272710-53272729	MS.gene97914:CDS	50.0%
	TAGCCCTGCATGGTATGCAA+TGG	+	chr6.1:53272717-53272736	None:intergenic	50.0%
	TGTCAACGGTGGGGATCTAT+TGG	-	chr6.1:53273009-53273028	MS.gene97914:CDS	50.0%
!	GGCGGCATATGATGATGCTA+TGG	-	chr6.1:53272595-53272614	MS.gene97914:CDS	50.0%
!	GTGTATCATTGGGATGGTGG+TGG	+	chr6.1:53272265-53272284	None:intergenic	50.0%
!!!	GGTGGTGGATTTTGAGGAGT+TGG	+	chr6.1:53272250-53272269	None:intergenic	50.0%
!!!	TGGGATGGTGGTGGATTTTG+AGG	+	chr6.1:53272256-53272275	None:intergenic	50.0%
	CAACTACACGTAGCCCTGCA+TGG	+	chr6.1:53272727-53272746	None:intergenic	55.0%
	CATCATATGCCGCCGGTAAC+CGG	+	chr6.1:53272589-53272608	None:intergenic	55.0%
	GGATCAAGGGTGGAGGCTAT+TGG	-	chr6.1:53272982-53273001	MS.gene97914:CDS	55.0%
	TAAGGATCGGTTGTCGGTGG+TGG	+	chr6.1:53272227-53272246	None:intergenic	55.0%
	TGGTAAGGATCGGTTGTCGG+TGG	+	chr6.1:53272230-53272249	None:intergenic	55.0%
	TGTCGAAGCAGTTGTCGCCT+CGG	-	chr6.1:53272538-53272557	MS.gene97914:CDS	55.0%
	TGCGAGGCGATAATCCACCG+AGG	+	chr6.1:53272558-53272577	None:intergenic	60.0%
!!	GGTGGATTATCGCCTCGCAC+CGG	-	chr6.1:53272559-53272578	MS.gene97914:CDS	60.0%
	CGAAGCAGTTGTCGCCTCGG+TGG	-	chr6.1:53272541-53272560	MS.gene97914:CDS	65.0%
	ATCGCCTCGCACCGGAGCAC+CGG	-	chr6.1:53272567-53272586	MS.gene97914:CDS	70.0%
	GTAACCGGTGCTCCGGTGCG+AGG	+	chr6.1:53272574-53272593	None:intergenic	70.0%
!!	ACCGGAGCACCGGTTACCGG+CGG	-	chr6.1:53272577-53272596	MS.gene97914:CDS	70.0%
!!	CGCACCGGAGCACCGGTTAC+CGG	-	chr6.1:53272574-53272593	MS.gene97914:CDS	70.0%
	GCCGCCGGTAACCGGTGCTC+CGG	+	chr6.1:53272581-53272600	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.1	gene	53272218	53273195	53272218	ID=MS.gene97914
chr6.1	mRNA	53272218	53273195	53272218	ID=MS.gene97914.t1;Parent=MS.gene97914
chr6.1	exon	53272218	53273195	53272218	ID=MS.gene97914.t1.exon1;Parent=MS.gene97914.t1
chr6.1	CDS	53272218	53273195	53272218	ID=cds.MS.gene97914.t1;Parent=MS.gene97914.t1

Gene Sequence

>MS.gene97914

ATGCATCCACCACCGACAACCGATCCTTACCAACTCCTCAAAATCCACCACCATCCCAATGATACACTTACTCGATATTTTGAAGATCCACACACCTCACCTTCATTAGACACTTCTCTTCCTGTTCTTACCAAAGATCTTAACATCAACCAATCAAACCAAACATGGCTTCGATTATTCCTACCTAAAAAAGCAACAAATATTTCAAACCAAAATAACAAGTTACTGCCACTTATTGTTTTCTTTCATGGTAGTGGCTTCATCGTACAAAGTGCAGCCTCCACCAAATTTCATGAATTATGTGTTGATATGGCGGATACTGTCGAAGCAGTTGTCGCCTCGGTGGATTATCGCCTCGCACCGGAGCACCGGTTACCGGCGGCATATGATGATGCTATGGAAGCATTGAGTTTGATTAGGTCAAGCAAAGATGAATGGTTGACTAAATATGTTGACTATTCTAAGTGTTACCTAATGGGTAATAGTGCTGGAGCTACCATTGCATACCATGCAGGGCTACGTGTAGTTGAAAACGTGAATGATTTTGAGCCATTGAAGATCCAAGGGTTGATATTGCGTCAACCATTTTTTGGTGGGACCAATAGGACTGAATCAGAGTTGAGACTTGAGAATGATCCGGATTTTCCTTTGTGTGTTAGTGATTTGTGTTGGGAGTTGGCATTACCTATTGGAGTTGATCGTGATCATGAGTATTGCAATCCAATGGTTGAGATTGATGTTGATGAAAAATTAGATAAGATAAAGGATCAAGGGTGGAGGCTATTGGTGAGTGTCAACGGTGGGGATCTATTGGTTGATCGTGCAAAAGAGTTGGTGCAATTGATGGAGGATAAGGGTGTGGATGTTGTTAAGGATTTTCAAGAAGATGGTTGCCATGGAGTTGAGTTTTTTGAGCCATCTAAAGCAAAGAAATTTATTGAGTTAGTGAAGGGCTTCATTTCCACGTTCGATGCTTAA

Protein sequence

>MS.gene97914.t1

MHPPPTTDPYQLLKIHHHPNDTLTRYFEDPHTSPSLDTSLPVLTKDLNINQSNQTWLRLFLPKKATNISNQNNKLLPLIVFFHGSGFIVQSAASTKFHELCVDMADTVEAVVASVDYRLAPEHRLPAAYDDAMEALSLIRSSKDEWLTKYVDYSKCYLMGNSAGATIAYHAGLRVVENVNDFEPLKIQGLILRQPFFGGTNRTESELRLENDPDFPLCVSDLCWELALPIGVDRDHEYCNPMVEIDVDEKLDKIKDQGWRLLVSVNGGDLLVDRAKELVQLMEDKGVDVVKDFQEDGCHGVEFFEPSKAKKFIELVKGFISTFDA