AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053470.t1 MTR_4g086510 96.262 321 12 0 1 321 1 321 0.0 636
MS.gene053470.t1 MTR_7g056207 85.489 317 46 0 2 318 34 350 0.0 568
MS.gene053470.t1 MTR_4g086480 64.798 321 106 3 1 317 1 318 6.37e-160 449
MS.gene053470.t1 MTR_4g086470 62.229 323 116 3 1 320 1 320 2.20e-144 410
MS.gene053470.t1 MTR_4g086540 45.570 316 149 4 2 317 12 304 1.62e-94 283
MS.gene053470.t1 MTR_5g005850 44.713 331 164 9 1 317 3 328 2.46e-94 283
MS.gene053470.t1 MTR_3g101420 43.354 316 165 6 9 318 8 315 1.37e-92 278
MS.gene053470.t1 MTR_1g105020 44.172 326 169 8 1 317 1 322 8.50e-91 274
MS.gene053470.t1 MTR_3g450680 42.202 327 175 7 2 318 10 332 1.62e-82 253
MS.gene053470.t1 MTR_8g040860 41.066 319 177 7 7 318 15 329 1.23e-76 238
MS.gene053470.t1 MTR_5g005920 39.024 328 189 8 2 319 6 332 1.99e-76 237
MS.gene053470.t1 MTR_5g005900 40.785 331 181 8 1 319 1 328 1.39e-74 233
MS.gene053470.t1 MTR_1g104930 40.683 322 178 7 4 318 8 323 5.08e-70 221
MS.gene053470.t1 MTR_7g051020 39.752 322 184 7 4 318 8 326 1.16e-68 217
MS.gene053470.t1 MTR_1g104933 40.063 317 175 7 8 318 10 317 2.87e-61 198
MS.gene053470.t1 MTR_1g111280 38.750 320 172 9 8 313 26 335 7.61e-61 198
MS.gene053470.t1 MTR_1g012430 38.801 317 177 9 8 318 3 308 4.61e-57 187
MS.gene053470.t1 MTR_7g406880 36.928 306 167 9 15 299 28 328 1.46e-55 184
MS.gene053470.t1 MTR_4g050140 34.877 324 190 8 5 317 2 315 2.61e-55 182
MS.gene053470.t1 MTR_7g107040 33.754 317 196 7 8 317 7 316 1.31e-46 160
MS.gene053470.t1 MTR_1g102160 35.231 281 157 7 16 279 19 291 5.12e-45 156
MS.gene053470.t1 MTR_8g035520 34.824 313 186 8 16 318 21 325 6.14e-43 150
MS.gene053470.t1 MTR_6g464120 36.585 287 158 10 21 294 29 304 7.14e-43 155
MS.gene053470.t1 MTR_6g464280 34.831 267 152 7 35 288 38 295 1.83e-42 149
MS.gene053470.t1 MTR_6g464510 34.628 309 176 11 24 318 31 327 4.50e-42 148
MS.gene053470.t1 MTR_1g102170 36.059 269 149 9 21 276 43 301 8.64e-42 148
MS.gene053470.t1 MTR_8g035540 34.494 316 185 9 16 317 9 316 9.24e-42 147
MS.gene053470.t1 MTR_6g065110 35.616 292 168 8 39 319 54 336 3.56e-41 146
MS.gene053470.t1 MTR_6g464320 32.270 282 169 8 35 302 39 312 6.51e-41 145
MS.gene053470.t1 MTR_0007s0520 31.045 335 197 11 1 320 12 327 4.13e-40 143
MS.gene053470.t1 MTR_6g065120 33.670 297 174 8 35 318 50 336 1.19e-39 142
MS.gene053470.t1 MTR_3g012440 30.321 343 207 10 4 318 3 341 1.36e-39 142
MS.gene053470.t1 MTR_6g065430 31.270 307 191 8 21 317 31 327 2.18e-36 134
MS.gene053470.t1 MTR_6g065340 31.849 292 169 6 35 318 60 329 2.98e-36 133
MS.gene053470.t1 MTR_1g089310 32.602 319 179 11 21 318 38 341 3.97e-36 133
MS.gene053470.t1 MTR_1g089310 32.602 319 179 11 21 318 54 357 4.80e-36 133
MS.gene053470.t1 MTR_7g093950 33.007 306 174 10 33 318 53 347 6.71e-36 132
MS.gene053470.t1 MTR_8g035500 31.890 254 161 5 35 279 33 283 4.63e-35 130
MS.gene053470.t1 MTR_6g065270 30.769 299 183 7 35 318 47 336 7.39e-35 130
MS.gene053470.t1 MTR_6g065370 30.169 295 186 6 35 318 49 334 2.63e-34 128
MS.gene053470.t1 MTR_6g065400 30.508 295 185 6 35 318 49 334 2.83e-34 128
MS.gene053470.t1 MTR_1g082210 30.464 302 181 8 33 318 53 341 8.43e-34 127
MS.gene053470.t1 MTR_6g022710 26.354 277 163 8 61 300 146 418 5.08e-18 84.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053470.t1 AT3G48700 49.848 329 151 6 1 318 1 326 1.06e-108 320
MS.gene053470.t1 AT3G48690 50.920 326 147 7 1 318 1 321 8.53e-107 315
MS.gene053470.t1 AT2G03550 49.679 312 151 3 8 318 5 311 4.85e-106 312
MS.gene053470.t1 AT1G47480 46.729 321 159 6 1 319 1 311 1.48e-102 303
MS.gene053470.t1 AT1G19190 48.447 322 154 6 1 318 1 314 1.04e-101 301
MS.gene053470.t1 AT1G49660 46.349 315 166 3 6 317 3 317 2.92e-94 283
MS.gene053470.t1 AT1G49650 47.078 308 159 4 14 318 67 373 5.99e-91 276
MS.gene053470.t1 AT1G49640 46.753 308 154 6 14 317 12 313 1.18e-87 265
MS.gene053470.t1 AT1G68620 36.250 320 185 8 8 319 23 331 1.32e-56 186
MS.gene053470.t1 AT5G16080 37.748 302 171 9 8 300 28 321 4.44e-55 183
MS.gene053470.t1 AT2G45600 32.177 317 185 9 16 318 16 316 3.59e-39 141
MS.gene053470.t1 AT5G06570 32.817 323 203 7 7 319 9 327 1.24e-38 139
MS.gene053470.t1 AT5G06570 32.817 323 203 7 7 319 9 327 1.24e-38 139
MS.gene053470.t1 AT5G06570 32.817 323 203 7 7 319 24 342 2.77e-38 139
MS.gene053470.t1 AT5G06570 32.817 323 203 7 7 319 24 342 2.77e-38 139
MS.gene053470.t1 AT5G62180 32.302 291 171 8 21 295 21 301 3.35e-38 138
MS.gene053470.t1 AT2G45610 32.063 315 195 9 16 318 17 324 1.13e-35 131
MS.gene053470.t1 AT3G05120 30.189 318 189 9 21 318 38 342 4.99e-35 130
MS.gene053470.t1 AT5G23530 31.773 299 172 10 33 319 50 328 1.99e-34 128
MS.gene053470.t1 AT5G27320 30.565 301 181 9 33 318 53 340 1.08e-33 127
MS.gene053470.t1 AT3G63010 33.200 250 148 7 75 318 106 342 1.25e-32 124

Find 87 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAACCGTTATGCAGATTT+TGG 0.137747 4.3:+26296879 MS.gene053470:CDS
TATCTTTGGCAGATAATTTA+TGG 0.167740 4.3:+26297075 MS.gene053470:CDS
TCCTGGAAAGGATAAGAATT+TGG 0.204845 4.3:+26297145 MS.gene053470:CDS
CCCAAATTCTTATCCTTTCC+AGG 0.230563 4.3:-26297146 None:intergenic
GAGATAGCGTTATCACAATT+TGG 0.257012 4.3:-26297329 None:intergenic
AAGGAGTTGCTTGAGAAAAT+TGG 0.262640 4.3:+26297242 MS.gene053470:CDS
AAACAAGGAGAGGGAGCTTT+TGG 0.316467 4.3:-26296644 None:intergenic
GAAATCTGAACATTTATCTT+TGG 0.318877 4.3:+26297061 MS.gene053470:CDS
TTGGATTCTACGTTTGTTGT+TGG 0.322208 4.3:-26296543 None:intergenic
CCTGGAAAGGATAAGAATTT+GGG 0.324352 4.3:+26297146 MS.gene053470:CDS
AGATTCTGACGGATATGTTT+TGG 0.326819 4.3:-26296587 None:intergenic
GGACAGAGGTTGGTATTATA+AGG 0.329561 4.3:+26297223 MS.gene053470:CDS
GGATAAGAATTTGGGGAGGT+TGG 0.340905 4.3:+26297154 MS.gene053470:CDS
GTTGGGTTGTAAGAGAGTTT+TGG 0.343853 4.3:+26297172 MS.gene053470:CDS
TTTGTTTGTCCAACAAGTTC+GGG 0.347347 4.3:+26297101 MS.gene053470:CDS
AATTGATCGTTGTTGGGTTT+GGG 0.349087 4.3:-26296615 None:intergenic
TTTGGAAAAGTGTTCTTTGC+AGG 0.354718 4.3:+26296897 MS.gene053470:CDS
TAACCTTGAAGGGATTGTTT+TGG 0.366201 4.3:+26296989 MS.gene053470:CDS
TTAACCACTGTGATTAATAA+AGG 0.374288 4.3:-26297372 None:intergenic
AAATTGATCGTTGTTGGGTT+TGG 0.376453 4.3:-26296616 None:intergenic
GATAAGAATTTGGGGAGGTT+GGG 0.378686 4.3:+26297155 MS.gene053470:CDS
GTTTGTTTGTCCAACAAGTT+CGG 0.387947 4.3:+26297100 MS.gene053470:CDS
GTTTGGAAATTGATCGTTGT+TGG 0.395971 4.3:-26296622 None:intergenic
ATTCATGGACTTCCCTCAAA+TGG 0.399432 4.3:+26296817 MS.gene053470:CDS
GGTTCTCATTTACATGGAAA+TGG 0.399768 4.3:+26296843 MS.gene053470:CDS
GCTATAGGAAGAGGATGTTC+TGG 0.403308 4.3:-26296786 None:intergenic
CATGAATCTTCGTATGCTAT+AGG 0.408799 4.3:-26296801 None:intergenic
CTATAGCATACGAAGATTCA+TGG 0.419285 4.3:+26296802 MS.gene053470:CDS
TTGCTTGAGAAAATTGGTTA+TGG 0.425920 4.3:+26297248 MS.gene053470:CDS
TAATCATGGGTGAGAGATCA+AGG 0.441310 4.3:-26296455 None:intergenic
TTTGTTGTTGGATCAAAAGA+TGG 0.442349 4.3:-26296531 None:intergenic
CCTCTCCTTGTTTACTTCCA+CGG 0.454808 4.3:+26296654 MS.gene053470:CDS
ATGGAAATGGTTCCGATGAA+TGG 0.465752 4.3:+26296856 MS.gene053470:CDS
GAACCAAAACAATCCCTTCA+AGG 0.467598 4.3:-26296992 None:intergenic
TCGTTAGAGCTTGAATCCAT+TGG 0.469914 4.3:-26296426 None:intergenic
GTTGGAACTGAAGGGCTTCA+AGG 0.471170 4.3:+26296963 MS.gene053470:CDS
TGGTGAAAGGGTTGGATCTG+AGG 0.472890 4.3:+26297031 MS.gene053470:CDS
CTTCAAGGGATTAACCTTGA+AGG 0.479794 4.3:+26296978 MS.gene053470:CDS
TTGTGGTAGGGTGGTGAGAA+AGG 0.481397 4.3:-26296699 None:intergenic
TTCTTTGCAGGAGACAGTGC+TGG 0.484895 4.3:+26296909 MS.gene053470:CDS
CTTGTTTACTTCCACGGTGG+TGG 0.494455 4.3:+26296660 MS.gene053470:CDS
TCATCGGATCCCGAACTTGT+TGG 0.500915 4.3:-26297110 None:intergenic
TTTGGAAATTGATCGTTGTT+GGG 0.501578 4.3:-26296621 None:intergenic
GGTCACATAGAAAGACTCAT+AGG 0.504720 4.3:+26296492 MS.gene053470:CDS
TTCAAGGGATTAACCTTGAA+GGG 0.506683 4.3:+26296979 MS.gene053470:CDS
CCTTTCCAGGATTCAAAAGC+GGG 0.506964 4.3:-26297133 None:intergenic
AATGAGAACCGACCCATTTG+AGG 0.508046 4.3:-26296830 None:intergenic
GCTATTCGTGTTGGAACTGA+AGG 0.508303 4.3:+26296954 MS.gene053470:CDS
TCTTCGTATGCTATAGGAAG+AGG 0.511339 4.3:-26296795 None:intergenic
TGCTGAGAATGATTCGTTGA+AGG 0.512607 4.3:+26297202 MS.gene053470:CDS
TGGGTCGGTTCTCATTTACA+TGG 0.515529 4.3:+26296837 MS.gene053470:CDS
ATGATTAAAATCTACAAAGA+TGG 0.516689 4.3:+26296471 MS.gene053470:CDS
TTCATGGACTTCCCTCAAAT+GGG 0.516932 4.3:+26296818 MS.gene053470:CDS
CTTTAGAAACAATGGTGTTG+AGG 0.518105 4.3:-26296725 None:intergenic
CTTGAGAAAATTGGTTATGG+TGG 0.520361 4.3:+26297251 MS.gene053470:CDS
AGGATTCAAAAGCGGGTCAT+CGG 0.531320 4.3:-26297126 None:intergenic
ATTCGTTGAAGGACAGAGGT+TGG 0.532870 4.3:+26297213 MS.gene053470:CDS
TTGGAACTGAAGGGCTTCAA+GGG 0.535779 4.3:+26296964 MS.gene053470:CDS
CAATGGTGTTGAGGAAATTG+TGG 0.547639 4.3:-26296716 None:intergenic
CACATTAGCTTTAGAAACAA+TGG 0.549484 4.3:-26296733 None:intergenic
AAAAGATGGAGGTACGATGT+TGG 0.553050 4.3:-26296517 None:intergenic
CATCACATGGCTATTCGTGT+TGG 0.555895 4.3:+26296945 MS.gene053470:CDS
TTGAGGAAATTGTGGTAGGG+TGG 0.557588 4.3:-26296708 None:intergenic
ATGAGAACCGACCCATTTGA+GGG 0.557631 4.3:-26296829 None:intergenic
AATTGGTTATGGTGGTGTTG+TGG 0.565299 4.3:+26297259 MS.gene053470:CDS
AGCAAACATTGCACATCACA+TGG 0.575653 4.3:+26296932 MS.gene053470:CDS
TGGACTTCCCTCAAATGGGT+CGG 0.583373 4.3:+26296822 MS.gene053470:CDS
CTCCTTGTTTACTTCCACGG+TGG 0.586065 4.3:+26296657 MS.gene053470:CDS
GTGGAAGTGATTGAGACAAA+AGG 0.600674 4.3:+26297278 MS.gene053470:CDS
CTATTCGTGTTGGAACTGAA+GGG 0.601762 4.3:+26296955 MS.gene053470:CDS
CACCACCGTGGAAGTAAACA+AGG 0.602557 4.3:-26296659 None:intergenic
GTGATTGAGACAAAAGGTGA+AGG 0.603450 4.3:+26297284 MS.gene053470:CDS
TCCTTTCCAGGATTCAAAAG+CGG 0.605233 4.3:-26297134 None:intergenic
GGTGTTGAGGAAATTGTGGT+AGG 0.613284 4.3:-26296712 None:intergenic
GTTGTTGGATCAAAAGATGG+AGG 0.615289 4.3:-26296528 None:intergenic
CGTGGAAGTAAACAAGGAGA+GGG 0.624111 4.3:-26296653 None:intergenic
GTGTTGAGGAAATTGTGGTA+GGG 0.625602 4.3:-26296711 None:intergenic
CCGTGGAAGTAAACAAGGAG+AGG 0.629043 4.3:-26296654 None:intergenic
ATAACGGTTGATCCATTCAT+CGG 0.634770 4.3:-26296868 None:intergenic
TTTGGGAATGAAGATTCTGA+CGG 0.635421 4.3:-26296598 None:intergenic
AATGATTCGTTGAAGGACAG+AGG 0.637660 4.3:+26297209 MS.gene053470:CDS
CTTTGGCAGATAATTTATGG+AGG 0.639376 4.3:+26297078 MS.gene053470:CDS
TGATTGAGACAAAAGGTGAA+GGG 0.642095 4.3:+26297285 MS.gene053470:CDS
GTTTCCTTTATTAATCACAG+TGG 0.666843 4.3:+26297368 MS.gene053470:CDS
CTGGAAAGGATAAGAATTTG+GGG 0.670967 4.3:+26297147 MS.gene053470:CDS
GAAAGGATAAGAATTTGGGG+AGG 0.711471 4.3:+26297150 MS.gene053470:CDS
CTACGCAGAAACCACCACCG+TGG 0.793795 4.3:-26296671 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGATTAAAATCTACAAAGA+TGG + chr4.3:26296471-26296490 MS.gene053470:CDS 20.0%
!!! ATCTTTGTAGATTTTAATCA+TGG - chr4.3:26296472-26296491 None:intergenic 20.0%
!!! TCTTTGTAGATTTTAATCAT+GGG - chr4.3:26296471-26296490 None:intergenic 20.0%
! GAAATCTGAACATTTATCTT+TGG + chr4.3:26297061-26297080 MS.gene053470:CDS 25.0%
! TATCTTTGGCAGATAATTTA+TGG + chr4.3:26297075-26297094 MS.gene053470:CDS 25.0%
! TTAACCACTGTGATTAATAA+AGG - chr4.3:26297375-26297394 None:intergenic 25.0%
!!! TTTGGTTCATACATTCTTTT+GGG + chr4.3:26297007-26297026 MS.gene053470:CDS 25.0%
!!! TTTTGGTTCATACATTCTTT+TGG + chr4.3:26297006-26297025 MS.gene053470:CDS 25.0%
GTTTCCTTTATTAATCACAG+TGG + chr4.3:26297368-26297387 MS.gene053470:CDS 30.0%
TTGCTTGAGAAAATTGGTTA+TGG + chr4.3:26297248-26297267 MS.gene053470:CDS 30.0%
TTTGGAAATTGATCGTTGTT+GGG - chr4.3:26296624-26296643 None:intergenic 30.0%
! CTTTTCCAAAATCTGCATAA+CGG - chr4.3:26296887-26296906 None:intergenic 30.0%
! TTGGTTCATACATTCTTTTG+GGG + chr4.3:26297008-26297027 MS.gene053470:CDS 30.0%
!! CACATTAGCTTTAGAAACAA+TGG - chr4.3:26296736-26296755 None:intergenic 30.0%
!! TTTGTTGTTGGATCAAAAGA+TGG - chr4.3:26296534-26296553 None:intergenic 30.0%
!!! TTTTGAGATGAGAATGTCTT+TGG - chr4.3:26296565-26296584 None:intergenic 30.0%
AAATTGATCGTTGTTGGGTT+TGG - chr4.3:26296619-26296638 None:intergenic 35.0%
AAGGAGTTGCTTGAGAAAAT+TGG + chr4.3:26297242-26297261 MS.gene053470:CDS 35.0%
AATTGATCGTTGTTGGGTTT+GGG - chr4.3:26296618-26296637 None:intergenic 35.0%
ATAACGGTTGATCCATTCAT+CGG - chr4.3:26296871-26296890 None:intergenic 35.0%
CATGAATCTTCGTATGCTAT+AGG - chr4.3:26296804-26296823 None:intergenic 35.0%
CCTGGAAAGGATAAGAATTT+GGG + chr4.3:26297146-26297165 MS.gene053470:CDS 35.0%
CTATAGCATACGAAGATTCA+TGG + chr4.3:26296802-26296821 MS.gene053470:CDS 35.0%
CTGGAAAGGATAAGAATTTG+GGG + chr4.3:26297147-26297166 MS.gene053470:CDS 35.0%
CTTGAGAAAATTGGTTATGG+TGG + chr4.3:26297251-26297270 MS.gene053470:CDS 35.0%
CTTTGGCAGATAATTTATGG+AGG + chr4.3:26297078-26297097 MS.gene053470:CDS 35.0%
GAGATAGCGTTATCACAATT+TGG - chr4.3:26297332-26297351 None:intergenic 35.0%
GGTTCTCATTTACATGGAAA+TGG + chr4.3:26296843-26296862 MS.gene053470:CDS 35.0%
GTTTGGAAATTGATCGTTGT+TGG - chr4.3:26296625-26296644 None:intergenic 35.0%
GTTTGTTTGTCCAACAAGTT+CGG + chr4.3:26297100-26297119 MS.gene053470:CDS 35.0%
TCCTGGAAAGGATAAGAATT+TGG + chr4.3:26297145-26297164 MS.gene053470:CDS 35.0%
TGATTGAGACAAAAGGTGAA+GGG + chr4.3:26297285-26297304 MS.gene053470:CDS 35.0%
TTCAAGGGATTAACCTTGAA+GGG + chr4.3:26296979-26296998 MS.gene053470:CDS 35.0%
TTTGGGAATGAAGATTCTGA+CGG - chr4.3:26296601-26296620 None:intergenic 35.0%
TTTGTTTGTCCAACAAGTTC+GGG + chr4.3:26297101-26297120 MS.gene053470:CDS 35.0%
! ATCAACCGTTATGCAGATTT+TGG + chr4.3:26296879-26296898 MS.gene053470:CDS 35.0%
! CTTTAGAAACAATGGTGTTG+AGG - chr4.3:26296728-26296747 None:intergenic 35.0%
! TAACCTTGAAGGGATTGTTT+TGG + chr4.3:26296989-26297008 MS.gene053470:CDS 35.0%
!! AGATTCTGACGGATATGTTT+TGG - chr4.3:26296590-26296609 None:intergenic 35.0%
!! TTGGATTCTACGTTTGTTGT+TGG - chr4.3:26296546-26296565 None:intergenic 35.0%
!! TTTGGAAAAGTGTTCTTTGC+AGG + chr4.3:26296897-26296916 MS.gene053470:CDS 35.0%
AATGATTCGTTGAAGGACAG+AGG + chr4.3:26297209-26297228 MS.gene053470:CDS 40.0%
AGCAAACATTGCACATCACA+TGG + chr4.3:26296932-26296951 MS.gene053470:CDS 40.0%
ATGGAAATGGTTCCGATGAA+TGG + chr4.3:26296856-26296875 MS.gene053470:CDS 40.0%
ATTCATGGACTTCCCTCAAA+TGG + chr4.3:26296817-26296836 MS.gene053470:CDS 40.0%
CCCAAATTCTTATCCTTTCC+AGG - chr4.3:26297149-26297168 None:intergenic 40.0%
CTATTCGTGTTGGAACTGAA+GGG + chr4.3:26296955-26296974 MS.gene053470:CDS 40.0%
CTTCAAGGGATTAACCTTGA+AGG + chr4.3:26296978-26296997 MS.gene053470:CDS 40.0%
GAAAGGATAAGAATTTGGGG+AGG + chr4.3:26297150-26297169 MS.gene053470:CDS 40.0%
GAACCAAAACAATCCCTTCA+AGG - chr4.3:26296995-26297014 None:intergenic 40.0%
GATAAGAATTTGGGGAGGTT+GGG + chr4.3:26297155-26297174 MS.gene053470:CDS 40.0%
GGTCACATAGAAAGACTCAT+AGG + chr4.3:26296492-26296511 MS.gene053470:CDS 40.0%
GTGATTGAGACAAAAGGTGA+AGG + chr4.3:26297284-26297303 MS.gene053470:CDS 40.0%
GTGGAAGTGATTGAGACAAA+AGG + chr4.3:26297278-26297297 MS.gene053470:CDS 40.0%
GTGTTGAGGAAATTGTGGTA+GGG - chr4.3:26296714-26296733 None:intergenic 40.0%
GTTGTTGGATCAAAAGATGG+AGG - chr4.3:26296531-26296550 None:intergenic 40.0%
TAATCATGGGTGAGAGATCA+AGG - chr4.3:26296458-26296477 None:intergenic 40.0%
TCCTTTCCAGGATTCAAAAG+CGG - chr4.3:26297137-26297156 None:intergenic 40.0%
TCTTCGTATGCTATAGGAAG+AGG - chr4.3:26296798-26296817 None:intergenic 40.0%
TGCTGAGAATGATTCGTTGA+AGG + chr4.3:26297202-26297221 MS.gene053470:CDS 40.0%
TTCATGGACTTCCCTCAAAT+GGG + chr4.3:26296818-26296837 MS.gene053470:CDS 40.0%
! AAAAGATGGAGGTACGATGT+TGG - chr4.3:26296520-26296539 None:intergenic 40.0%
! AATTGGTTATGGTGGTGTTG+TGG + chr4.3:26297259-26297278 MS.gene053470:CDS 40.0%
! CAATGGTGTTGAGGAAATTG+TGG - chr4.3:26296719-26296738 None:intergenic 40.0%
!! GGACAGAGGTTGGTATTATA+AGG + chr4.3:26297223-26297242 MS.gene053470:CDS 40.0%
!! GTTCATACATTCTTTTGGGG+TGG + chr4.3:26297011-26297030 MS.gene053470:CDS 40.0%
!! GTTGGGTTGTAAGAGAGTTT+TGG + chr4.3:26297172-26297191 MS.gene053470:CDS 40.0%
!!! ATTCTTTTGGGGTGGTGAAA+GGG + chr4.3:26297019-26297038 MS.gene053470:CDS 40.0%
AATGAGAACCGACCCATTTG+AGG - chr4.3:26296833-26296852 None:intergenic 45.0%
AGGATTCAAAAGCGGGTCAT+CGG - chr4.3:26297129-26297148 None:intergenic 45.0%
ATGAGAACCGACCCATTTGA+GGG - chr4.3:26296832-26296851 None:intergenic 45.0%
ATTCGTTGAAGGACAGAGGT+TGG + chr4.3:26297213-26297232 MS.gene053470:CDS 45.0%
CATCACATGGCTATTCGTGT+TGG + chr4.3:26296945-26296964 MS.gene053470:CDS 45.0%
CCTTTCCAGGATTCAAAAGC+GGG - chr4.3:26297136-26297155 None:intergenic 45.0%
CGTGGAAGTAAACAAGGAGA+GGG - chr4.3:26296656-26296675 None:intergenic 45.0%
GCTATAGGAAGAGGATGTTC+TGG - chr4.3:26296789-26296808 None:intergenic 45.0%
GCTATTCGTGTTGGAACTGA+AGG + chr4.3:26296954-26296973 MS.gene053470:CDS 45.0%
GGATAAGAATTTGGGGAGGT+TGG + chr4.3:26297154-26297173 MS.gene053470:CDS 45.0%
TTGAGGAAATTGTGGTAGGG+TGG - chr4.3:26296711-26296730 None:intergenic 45.0%
TTGGAACTGAAGGGCTTCAA+GGG + chr4.3:26296964-26296983 MS.gene053470:CDS 45.0%
! AAACAAGGAGAGGGAGCTTT+TGG - chr4.3:26296647-26296666 None:intergenic 45.0%
! CCTCTCCTTGTTTACTTCCA+CGG + chr4.3:26296654-26296673 MS.gene053470:CDS 45.0%
! GGTGTTGAGGAAATTGTGGT+AGG - chr4.3:26296715-26296734 None:intergenic 45.0%
! TGGGTCGGTTCTCATTTACA+TGG + chr4.3:26296837-26296856 MS.gene053470:CDS 45.0%
!!! CATTCTTTTGGGGTGGTGAA+AGG + chr4.3:26297018-26297037 MS.gene053470:CDS 45.0%
CACCACCGTGGAAGTAAACA+AGG - chr4.3:26296662-26296681 None:intergenic 50.0%
CCGTGGAAGTAAACAAGGAG+AGG - chr4.3:26296657-26296676 None:intergenic 50.0%
CTTGTTTACTTCCACGGTGG+TGG + chr4.3:26296660-26296679 MS.gene053470:CDS 50.0%
GTTGGAACTGAAGGGCTTCA+AGG + chr4.3:26296963-26296982 MS.gene053470:CDS 50.0%
TCATCGGATCCCGAACTTGT+TGG - chr4.3:26297113-26297132 None:intergenic 50.0%
TGGACTTCCCTCAAATGGGT+CGG + chr4.3:26296822-26296841 MS.gene053470:CDS 50.0%
TGGTGAAAGGGTTGGATCTG+AGG + chr4.3:26297031-26297050 MS.gene053470:CDS 50.0%
TTCTTTGCAGGAGACAGTGC+TGG + chr4.3:26296909-26296928 MS.gene053470:CDS 50.0%
TTGTGGTAGGGTGGTGAGAA+AGG - chr4.3:26296702-26296721 None:intergenic 50.0%
! CTCCTTGTTTACTTCCACGG+TGG + chr4.3:26296657-26296676 MS.gene053470:CDS 50.0%
!! AGGAGAGGGAGCTTTTGGTT+TGG - chr4.3:26296642-26296661 None:intergenic 50.0%
!! CCCGCTTTTGAATCCTGGAA+AGG + chr4.3:26297133-26297152 MS.gene053470:CDS 50.0%
!! GATGACCCGCTTTTGAATCC+TGG + chr4.3:26297128-26297147 MS.gene053470:CDS 50.0%
!!! TTTTGGGGTGGTGAAAGGGT+TGG + chr4.3:26297023-26297042 MS.gene053470:CDS 50.0%
CTACGCAGAAACCACCACCG+TGG - chr4.3:26296674-26296693 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 26296429 26297394 26296429 ID=MS.gene053470
chr4.3 mRNA 26296429 26297394 26296429 ID=MS.gene053470.t1;Parent=MS.gene053470
chr4.3 exon 26296429 26297394 26296429 ID=MS.gene053470.t1.exon1;Parent=MS.gene053470.t1
chr4.3 CDS 26296429 26297394 26296429 ID=cds.MS.gene053470.t1;Parent=MS.gene053470.t1
Gene Sequence

>MS.gene053470

ATGGATTCAAGCTCTAACGAAGTAGTCCTTGATCTCTCACCCATGATTAAAATCTACAAAGATGGTCACATAGAAAGACTCATAGGATCCAACATCGTACCTCCATCTTTTGATCCAACAACAAACGTAGAATCCAAAGACATTCTCATCTCAAAAGACCAAAACATATCCGTCAGAATCTTCATTCCCAAACCCAACAACGATCAATTTCCAAACCAAAAGCTCCCTCTCCTTGTTTACTTCCACGGTGGTGGTTTCTGCGTAGAAACACCTTTCTCACCACCCTACCACAATTTCCTCAACACCATTGTTTCTAAAGCTAATGTGATCGCTGTTTCAGTTGATTACAGAAGAGCACCAGAACATCCTCTTCCTATAGCATACGAAGATTCATGGACTTCCCTCAAATGGGTCGGTTCTCATTTACATGGAAATGGTTCCGATGAATGGATCAACCGTTATGCAGATTTTGGAAAAGTGTTCTTTGCAGGAGACAGTGCTGGAGCAAACATTGCACATCACATGGCTATTCGTGTTGGAACTGAAGGGCTTCAAGGGATTAACCTTGAAGGGATTGTTTTGGTTCATACATTCTTTTGGGGTGGTGAAAGGGTTGGATCTGAGGCTGCTGAGAAATCTGAACATTTATCTTTGGCAGATAATTTATGGAGGTTTGTTTGTCCAACAAGTTCGGGATCCGATGACCCGCTTTTGAATCCTGGAAAGGATAAGAATTTGGGGAGGTTGGGTTGTAAGAGAGTTTTGGTTTGTGTTGCTGAGAATGATTCGTTGAAGGACAGAGGTTGGTATTATAAGGAGTTGCTTGAGAAAATTGGTTATGGTGGTGTTGTGGAAGTGATTGAGACAAAAGGTGAAGGGCATGTTTTTCATCTCTTTGATCCAAATTGTGATAACGCTATCTCTTTGCTTAATCAAATTGTTTCCTTTATTAATCACAGTGGTTAA
Protein sequence

>MS.gene053470.t1

MDSSSNEVVLDLSPMIKIYKDGHIERLIGSNIVPPSFDPTTNVESKDILISKDQNISVRIFIPKPNNDQFPNQKLPLLVYFHGGGFCVETPFSPPYHNFLNTIVSKANVIAVSVDYRRAPEHPLPIAYEDSWTSLKWVGSHLHGNGSDEWINRYADFGKVFFAGDSAGANIAHHMAIRVGTEGLQGINLEGIVLVHTFFWGGERVGSEAAEKSEHLSLADNLWRFVCPTSSGSDDPLLNPGKDKNLGRLGCKRVLVCVAENDSLKDRGWYYKELLEKIGYGGVVEVIETKGEGHVFHLFDPNCDNAISLLNQIVSFINHSG