AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene047802

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene047802.t1 MTR_4g086510 96.573 321 11 0 1 321 1 321 0.0 640
MS.gene047802.t1 MTR_7g056207 85.759 316 45 0 2 317 34 349 0.0 568
MS.gene047802.t1 MTR_4g086480 64.907 322 106 3 1 318 1 319 1.41e-161 453
MS.gene047802.t1 MTR_4g086470 62.539 323 115 3 1 320 1 320 1.31e-145 413
MS.gene047802.t1 MTR_5g005850 45.015 331 163 9 1 317 3 328 4.28e-95 285
MS.gene047802.t1 MTR_4g086540 45.253 316 150 4 2 317 12 304 1.11e-93 281
MS.gene047802.t1 MTR_3g101420 43.810 315 163 6 9 317 8 314 2.14e-93 280
MS.gene047802.t1 MTR_1g105020 44.172 326 169 8 1 317 1 322 9.79e-91 273
MS.gene047802.t1 MTR_3g450680 42.202 327 175 7 2 318 10 332 3.45e-83 254
MS.gene047802.t1 MTR_8g040860 40.752 319 178 7 7 318 15 329 1.52e-76 238
MS.gene047802.t1 MTR_5g005920 38.415 328 191 8 2 319 6 332 7.60e-76 236
MS.gene047802.t1 MTR_5g005900 41.337 329 178 8 1 317 1 326 1.64e-75 235
MS.gene047802.t1 MTR_1g104930 41.121 321 176 7 4 317 8 322 6.05e-71 223
MS.gene047802.t1 MTR_7g051020 40.187 321 182 7 4 317 8 325 1.60e-69 219
MS.gene047802.t1 MTR_1g104933 40.506 316 173 7 8 317 10 316 3.38e-62 200
MS.gene047802.t1 MTR_1g111280 40.065 307 160 9 8 300 26 322 8.93e-61 198
MS.gene047802.t1 MTR_1g012430 39.117 317 176 9 8 318 3 308 1.60e-58 191
MS.gene047802.t1 MTR_4g050140 34.462 325 192 8 5 318 2 316 1.73e-55 183
MS.gene047802.t1 MTR_7g406880 36.928 306 167 9 15 299 28 328 2.23e-55 184
MS.gene047802.t1 MTR_7g107040 33.125 320 200 7 8 320 7 319 1.46e-46 160
MS.gene047802.t1 MTR_1g102160 35.231 281 157 7 16 279 19 291 3.65e-45 156
MS.gene047802.t1 MTR_6g464120 36.585 287 158 10 21 294 29 304 8.81e-44 157
MS.gene047802.t1 MTR_8g035520 34.936 312 185 8 16 317 21 324 1.03e-43 153
MS.gene047802.t1 MTR_8g035540 34.494 316 185 9 16 317 9 316 9.06e-43 150
MS.gene047802.t1 MTR_6g464280 34.831 267 152 7 35 288 38 295 1.36e-42 150
MS.gene047802.t1 MTR_6g464510 34.091 308 177 10 24 317 31 326 7.01e-42 148
MS.gene047802.t1 MTR_1g102170 36.059 269 149 9 21 276 43 301 2.03e-41 147
MS.gene047802.t1 MTR_6g464320 31.650 297 179 9 35 317 39 325 2.66e-41 146
MS.gene047802.t1 MTR_6g065110 35.274 292 169 8 39 319 54 336 5.20e-41 146
MS.gene047802.t1 MTR_6g065120 33.784 296 173 8 35 317 50 335 4.12e-40 144
MS.gene047802.t1 MTR_0007s0520 30.746 335 198 11 1 320 12 327 2.71e-39 141
MS.gene047802.t1 MTR_3g012440 30.029 343 208 9 4 318 3 341 3.36e-39 141
MS.gene047802.t1 MTR_6g065340 31.959 291 168 6 35 317 60 328 1.88e-36 134
MS.gene047802.t1 MTR_1g089310 32.704 318 178 11 21 317 38 340 3.22e-36 133
MS.gene047802.t1 MTR_6g065430 31.270 307 191 8 21 317 31 327 3.27e-36 133
MS.gene047802.t1 MTR_1g089310 32.704 318 178 11 21 317 54 356 4.11e-36 133
MS.gene047802.t1 MTR_6g065270 31.208 298 181 7 35 317 47 335 9.33e-36 132
MS.gene047802.t1 MTR_7g093950 33.010 309 168 11 33 317 53 346 2.29e-35 131
MS.gene047802.t1 MTR_8g035500 30.169 295 184 8 35 317 33 317 5.41e-35 129
MS.gene047802.t1 MTR_6g065370 30.272 294 185 6 35 317 49 333 2.01e-34 129
MS.gene047802.t1 MTR_6g065400 30.612 294 184 6 35 317 49 333 2.12e-34 128
MS.gene047802.t1 MTR_1g082210 30.233 301 181 8 33 317 53 340 9.16e-34 127
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene047802.t1 AT3G48700 50.152 329 150 6 1 318 1 326 2.30e-109 321
MS.gene047802.t1 AT3G48690 50.920 326 147 7 1 318 1 321 4.58e-107 315
MS.gene047802.t1 AT2G03550 50.000 312 148 4 8 317 5 310 2.29e-106 313
MS.gene047802.t1 AT1G19190 49.379 322 151 7 1 318 1 314 7.44e-103 304
MS.gene047802.t1 AT1G47480 46.729 321 159 6 1 319 1 311 6.50e-102 302
MS.gene047802.t1 AT1G49660 46.349 315 166 3 6 317 3 317 3.26e-94 282
MS.gene047802.t1 AT1G49650 47.231 307 158 4 14 317 67 372 3.60e-91 276
MS.gene047802.t1 AT1G49640 47.078 308 153 6 14 317 12 313 1.63e-88 268
MS.gene047802.t1 AT1G68620 36.562 320 184 8 8 319 23 331 2.09e-56 186
MS.gene047802.t1 AT5G16080 36.025 322 186 10 8 317 28 341 2.37e-54 181
MS.gene047802.t1 AT2G45600 32.492 317 184 9 16 318 16 316 7.18e-41 145
MS.gene047802.t1 AT5G06570 32.710 321 202 7 7 317 9 325 1.59e-38 139
MS.gene047802.t1 AT5G06570 32.710 321 202 7 7 317 9 325 1.59e-38 139
MS.gene047802.t1 AT5G06570 32.710 321 202 7 7 317 24 340 2.09e-38 139
MS.gene047802.t1 AT5G06570 32.710 321 202 7 7 317 24 340 2.09e-38 139
MS.gene047802.t1 AT5G62180 31.310 313 189 8 21 317 21 323 2.99e-38 138
MS.gene047802.t1 AT5G23530 31.987 297 170 10 33 317 50 326 1.21e-34 129
MS.gene047802.t1 AT3G05120 29.968 317 189 9 21 317 38 341 1.53e-34 129
MS.gene047802.t1 AT2G45610 31.847 314 195 9 16 317 17 323 1.56e-34 128
MS.gene047802.t1 AT5G27320 30.667 300 180 9 33 317 53 339 6.48e-34 127
MS.gene047802.t1 AT3G63010 33.333 249 147 7 75 317 106 341 4.93e-33 125

Find 88 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAACCGTTATGCAGATTT+TGG 0.137747 4.4:+25885645 MS.gene047802:CDS
TATCTTTGGCAGATAATTTA+TGG 0.167740 4.4:+25885841 MS.gene047802:CDS
TCCTGGAAAGGATAAGAATT+TGG 0.204845 4.4:+25885911 MS.gene047802:CDS
CCCAAATTCTTATCCTTTCC+AGG 0.230563 4.4:-25885912 None:intergenic
GAAGTAGCGTTATCACAATT+TGG 0.238391 4.4:-25886095 None:intergenic
AAGGAGTTGCTTGAGAAAAT+TGG 0.262640 4.4:+25886008 MS.gene047802:CDS
TTTCGAGATGAGAATGTCTT+TGG 0.280568 4.4:-25885328 None:intergenic
GAAATCTGAACATTTATCTT+TGG 0.318877 4.4:+25885827 MS.gene047802:CDS
TTGGATTCTACGTTTGTTGT+TGG 0.322208 4.4:-25885309 None:intergenic
CCTGGAAAGGATAAGAATTT+GGG 0.324352 4.4:+25885912 MS.gene047802:CDS
AGATTCTGACGGATATGTTT+TGG 0.326819 4.4:-25885353 None:intergenic
GGACAGAGGTTGGTATTATA+AGG 0.329561 4.4:+25885989 MS.gene047802:CDS
GGATAAGAATTTGGGGAGGT+TGG 0.340905 4.4:+25885920 MS.gene047802:CDS
GTTGGGTTGTAAGAGAGTTT+TGG 0.343853 4.4:+25885938 MS.gene047802:CDS
AATTGATCGTTGTTGGGTTT+GGG 0.349087 4.4:-25885381 None:intergenic
GCTATAGGAAGAGGATGTTC+TGG 0.353632 4.4:-25885552 None:intergenic
TTTGGAAAAGTGTTCTTTGC+AGG 0.354718 4.4:+25885663 MS.gene047802:CDS
TTTGTTTGTCCAACCAGTTC+GGG 0.355384 4.4:+25885867 MS.gene047802:CDS
TAACCTTGAAGGGATTGTTT+TGG 0.366201 4.4:+25885755 MS.gene047802:CDS
AAATTGATCGTTGTTGGGTT+TGG 0.376453 4.4:-25885382 None:intergenic
GATAAGAATTTGGGGAGGTT+GGG 0.378686 4.4:+25885921 MS.gene047802:CDS
TTAACCACTATGCTTGATAA+AGG 0.385755 4.4:-25886138 None:intergenic
GTTTGGAAATTGATCGTTGT+TGG 0.395971 4.4:-25885388 None:intergenic
GTTTGTTTGTCCAACCAGTT+CGG 0.396365 4.4:+25885866 MS.gene047802:CDS
ATTCATGGACTTCCCTCAAA+TGG 0.399432 4.4:+25885583 MS.gene047802:CDS
GGTTCTCATTTACATGGAAA+TGG 0.399768 4.4:+25885609 MS.gene047802:CDS
CATGAATCTTCGTATGCTAT+AGG 0.408799 4.4:-25885567 None:intergenic
CTATAGCATACGAAGATTCA+TGG 0.419285 4.4:+25885568 MS.gene047802:CDS
GTCCTTCAACGAATCATTCT+CGG 0.424168 4.4:-25885970 None:intergenic
TTGCTTGAGAAAATTGGTTA+TGG 0.425920 4.4:+25886014 MS.gene047802:CDS
TAATCATGGGTGAGAGATCA+AGG 0.441310 4.4:-25885221 None:intergenic
TTTGTTGTTGGATCAAAAGA+TGG 0.442349 4.4:-25885297 None:intergenic
CCTCTCCTTGTTTACTTCCA+CGG 0.464719 4.4:+25885420 MS.gene047802:CDS
CTATAGGAAGAGGATGTTCT+GGG 0.466651 4.4:-25885551 None:intergenic
GAACCAAAACAATCCCTTCA+AGG 0.467598 4.4:-25885758 None:intergenic
TCGTTAGAGCTTGAATCCAT+TGG 0.469914 4.4:-25885192 None:intergenic
GTTGGAACTGAAGGGCTTCA+AGG 0.471170 4.4:+25885729 MS.gene047802:CDS
TGTTGAAAGGGTTGGATCTG+AGG 0.476216 4.4:+25885797 MS.gene047802:CDS
CTTCAAGGGATTAACCTTGA+AGG 0.479794 4.4:+25885744 MS.gene047802:CDS
TGCCGAGAATGATTCGTTGA+AGG 0.481298 4.4:+25885968 MS.gene047802:CDS
CGGGTCATCGGATCCCGAAC+TGG 0.481764 4.4:-25885880 None:intergenic
TTCTTTGCAGGAGACAGTGC+TGG 0.484895 4.4:+25885675 MS.gene047802:CDS
CTTGTTTACTTCCACGGTGG+TGG 0.494455 4.4:+25885426 MS.gene047802:CDS
TTGTGGTATGGTGGTGAGAA+AGG 0.497082 4.4:-25885465 None:intergenic
ATGGAAATGGTTCTGATGAA+TGG 0.500867 4.4:+25885622 MS.gene047802:CDS
TTTGGAAATTGATCGTTGTT+GGG 0.501578 4.4:-25885387 None:intergenic
GGTCACATAGAAAGACTCAT+AGG 0.504720 4.4:+25885258 MS.gene047802:CDS
TTCAAGGGATTAACCTTGAA+GGG 0.506683 4.4:+25885745 MS.gene047802:CDS
CCTTTCCAGGATTCAAAAGC+GGG 0.506964 4.4:-25885899 None:intergenic
AATGAGAACCGACCCATTTG+AGG 0.508046 4.4:-25885596 None:intergenic
GCTATTCGTGTTGGAACTGA+AGG 0.508303 4.4:+25885720 MS.gene047802:CDS
TCTTCGTATGCTATAGGAAG+AGG 0.511339 4.4:-25885561 None:intergenic
TGGGTCGGTTCTCATTTACA+TGG 0.515529 4.4:+25885603 MS.gene047802:CDS
ATGATTAAAATCTACAAAGA+TGG 0.516689 4.4:+25885237 MS.gene047802:CDS
TTCATGGACTTCCCTCAAAT+GGG 0.516932 4.4:+25885584 MS.gene047802:CDS
GCTTCCTTTATCAAGCATAG+TGG 0.517973 4.4:+25886134 MS.gene047802:CDS
CTTTAGAAACAATGGTGTTG+AGG 0.518105 4.4:-25885491 None:intergenic
CTTGAGAAAATTGGTTATGG+TGG 0.520361 4.4:+25886017 MS.gene047802:CDS
CAATGGTGTTGAGGAAATTG+TGG 0.524537 4.4:-25885482 None:intergenic
AGGATTCAAAAGCGGGTCAT+CGG 0.531320 4.4:-25885892 None:intergenic
ATTCGTTGAAGGACAGAGGT+TGG 0.532870 4.4:+25885979 MS.gene047802:CDS
TTGGAACTGAAGGGCTTCAA+GGG 0.535779 4.4:+25885730 MS.gene047802:CDS
CACATTAGCTTTAGAAACAA+TGG 0.549484 4.4:-25885499 None:intergenic
AAAAGATGGAGGTACGATGT+TGG 0.553050 4.4:-25885283 None:intergenic
CATCACATGGCTATTCGTGT+TGG 0.555895 4.4:+25885711 MS.gene047802:CDS
ATGAGAACCGACCCATTTGA+GGG 0.557631 4.4:-25885595 None:intergenic
AATTGGTTATGGTGGTGTTG+TGG 0.565299 4.4:+25886025 MS.gene047802:CDS
AGCAAACATTGCACATCACA+TGG 0.575653 4.4:+25885698 MS.gene047802:CDS
TGGACTTCCCTCAAATGGGT+CGG 0.583373 4.4:+25885588 MS.gene047802:CDS
CTCCTTGTTTACTTCCACGG+TGG 0.586065 4.4:+25885423 MS.gene047802:CDS
TCATCGGATCCCGAACTGGT+TGG 0.599783 4.4:-25885876 None:intergenic
GTGGAAGTGATTGAGACAAA+AGG 0.600674 4.4:+25886044 MS.gene047802:CDS
CTATTCGTGTTGGAACTGAA+GGG 0.601762 4.4:+25885721 MS.gene047802:CDS
CACCACCGTGGAAGTAAACA+AGG 0.602557 4.4:-25885425 None:intergenic
TCCTTTCCAGGATTCAAAAG+CGG 0.605233 4.4:-25885900 None:intergenic
TTGAGGAAATTGTGGTATGG+TGG 0.607405 4.4:-25885474 None:intergenic
GTTGTTGGATCAAAAGATGG+AGG 0.615289 4.4:-25885294 None:intergenic
CCGTGGAAGTAAACAAGGAG+AGG 0.629043 4.4:-25885420 None:intergenic
CGTGGAAGTAAACAAGGAGA+GGG 0.633922 4.4:-25885419 None:intergenic
TTTGGGAATGAAGATTCTGA+CGG 0.635421 4.4:-25885364 None:intergenic
AATGATTCGTTGAAGGACAG+AGG 0.637660 4.4:+25885975 MS.gene047802:CDS
CTTTGGCAGATAATTTATGG+AGG 0.639376 4.4:+25885844 MS.gene047802:CDS
GTGATTGAGACAAAAGGTGA+AGG 0.640528 4.4:+25886050 MS.gene047802:CDS
GTGTTGAGGAAATTGTGGTA+TGG 0.652807 4.4:-25885477 None:intergenic
CTGGAAAGGATAAGAATTTG+GGG 0.670967 4.4:+25885913 MS.gene047802:CDS
GAAAGGATAAGAATTTGGGG+AGG 0.711471 4.4:+25885916 MS.gene047802:CDS
TATAGGAAGAGGATGTTCTG+GGG 0.732130 4.4:-25885550 None:intergenic
CTACGCAGAAACCACCACCG+TGG 0.793795 4.4:-25885437 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGATTAAAATCTACAAAGA+TGG + chr4.4:25885237-25885256 MS.gene047802:CDS 20.0%
!!! ATCTTTGTAGATTTTAATCA+TGG - chr4.4:25885238-25885257 None:intergenic 20.0%
!!! TCTTTGTAGATTTTAATCAT+GGG - chr4.4:25885237-25885256 None:intergenic 20.0%
! GAAATCTGAACATTTATCTT+TGG + chr4.4:25885827-25885846 MS.gene047802:CDS 25.0%
! TATCTTTGGCAGATAATTTA+TGG + chr4.4:25885841-25885860 MS.gene047802:CDS 25.0%
!!! TTTGGTTCATACATTCTTTT+GGG + chr4.4:25885773-25885792 MS.gene047802:CDS 25.0%
!!! TTTTGGTTCATACATTCTTT+TGG + chr4.4:25885772-25885791 MS.gene047802:CDS 25.0%
TTAACCACTATGCTTGATAA+AGG - chr4.4:25886141-25886160 None:intergenic 30.0%
TTGCTTGAGAAAATTGGTTA+TGG + chr4.4:25886014-25886033 MS.gene047802:CDS 30.0%
TTTGGAAATTGATCGTTGTT+GGG - chr4.4:25885390-25885409 None:intergenic 30.0%
! CTTTTCCAAAATCTGCATAA+CGG - chr4.4:25885653-25885672 None:intergenic 30.0%
! TTGGTTCATACATTCTTTTG+GGG + chr4.4:25885774-25885793 MS.gene047802:CDS 30.0%
!! CACATTAGCTTTAGAAACAA+TGG - chr4.4:25885502-25885521 None:intergenic 30.0%
!! TTTGTTGTTGGATCAAAAGA+TGG - chr4.4:25885300-25885319 None:intergenic 30.0%
AAATTGATCGTTGTTGGGTT+TGG - chr4.4:25885385-25885404 None:intergenic 35.0%
AAGGAGTTGCTTGAGAAAAT+TGG + chr4.4:25886008-25886027 MS.gene047802:CDS 35.0%
AATTGATCGTTGTTGGGTTT+GGG - chr4.4:25885384-25885403 None:intergenic 35.0%
CATGAATCTTCGTATGCTAT+AGG - chr4.4:25885570-25885589 None:intergenic 35.0%
CCTGGAAAGGATAAGAATTT+GGG + chr4.4:25885912-25885931 MS.gene047802:CDS 35.0%
CTATAGCATACGAAGATTCA+TGG + chr4.4:25885568-25885587 MS.gene047802:CDS 35.0%
CTGGAAAGGATAAGAATTTG+GGG + chr4.4:25885913-25885932 MS.gene047802:CDS 35.0%
CTTGAGAAAATTGGTTATGG+TGG + chr4.4:25886017-25886036 MS.gene047802:CDS 35.0%
CTTTGGCAGATAATTTATGG+AGG + chr4.4:25885844-25885863 MS.gene047802:CDS 35.0%
GAAGTAGCGTTATCACAATT+TGG - chr4.4:25886098-25886117 None:intergenic 35.0%
GGTTCTCATTTACATGGAAA+TGG + chr4.4:25885609-25885628 MS.gene047802:CDS 35.0%
GTTTGGAAATTGATCGTTGT+TGG - chr4.4:25885391-25885410 None:intergenic 35.0%
TCCTGGAAAGGATAAGAATT+TGG + chr4.4:25885911-25885930 MS.gene047802:CDS 35.0%
TTCAAGGGATTAACCTTGAA+GGG + chr4.4:25885745-25885764 MS.gene047802:CDS 35.0%
TTTCGAGATGAGAATGTCTT+TGG - chr4.4:25885331-25885350 None:intergenic 35.0%
TTTGGGAATGAAGATTCTGA+CGG - chr4.4:25885367-25885386 None:intergenic 35.0%
! ATCAACCGTTATGCAGATTT+TGG + chr4.4:25885645-25885664 MS.gene047802:CDS 35.0%
! CTTTAGAAACAATGGTGTTG+AGG - chr4.4:25885494-25885513 None:intergenic 35.0%
! TAACCTTGAAGGGATTGTTT+TGG + chr4.4:25885755-25885774 MS.gene047802:CDS 35.0%
!! AGATTCTGACGGATATGTTT+TGG - chr4.4:25885356-25885375 None:intergenic 35.0%
!! ATGGAAATGGTTCTGATGAA+TGG + chr4.4:25885622-25885641 MS.gene047802:CDS 35.0%
!! TTGGATTCTACGTTTGTTGT+TGG - chr4.4:25885312-25885331 None:intergenic 35.0%
!! TTTGGAAAAGTGTTCTTTGC+AGG + chr4.4:25885663-25885682 MS.gene047802:CDS 35.0%
!!! ATTCTTTTGGGGTGTTGAAA+GGG + chr4.4:25885785-25885804 MS.gene047802:CDS 35.0%
AATGATTCGTTGAAGGACAG+AGG + chr4.4:25885975-25885994 MS.gene047802:CDS 40.0%
AGCAAACATTGCACATCACA+TGG + chr4.4:25885698-25885717 MS.gene047802:CDS 40.0%
ATTCATGGACTTCCCTCAAA+TGG + chr4.4:25885583-25885602 MS.gene047802:CDS 40.0%
CCCAAATTCTTATCCTTTCC+AGG - chr4.4:25885915-25885934 None:intergenic 40.0%
CTATAGGAAGAGGATGTTCT+GGG - chr4.4:25885554-25885573 None:intergenic 40.0%
CTATTCGTGTTGGAACTGAA+GGG + chr4.4:25885721-25885740 MS.gene047802:CDS 40.0%
CTTCAAGGGATTAACCTTGA+AGG + chr4.4:25885744-25885763 MS.gene047802:CDS 40.0%
GAAAGGATAAGAATTTGGGG+AGG + chr4.4:25885916-25885935 MS.gene047802:CDS 40.0%
GAACCAAAACAATCCCTTCA+AGG - chr4.4:25885761-25885780 None:intergenic 40.0%
GATAAGAATTTGGGGAGGTT+GGG + chr4.4:25885921-25885940 MS.gene047802:CDS 40.0%
GCTTCCTTTATCAAGCATAG+TGG + chr4.4:25886134-25886153 MS.gene047802:CDS 40.0%
GGTCACATAGAAAGACTCAT+AGG + chr4.4:25885258-25885277 MS.gene047802:CDS 40.0%
GTCCTTCAACGAATCATTCT+CGG - chr4.4:25885973-25885992 None:intergenic 40.0%
GTGATTGAGACAAAAGGTGA+AGG + chr4.4:25886050-25886069 MS.gene047802:CDS 40.0%
GTGGAAGTGATTGAGACAAA+AGG + chr4.4:25886044-25886063 MS.gene047802:CDS 40.0%
GTGTTGAGGAAATTGTGGTA+TGG - chr4.4:25885480-25885499 None:intergenic 40.0%
GTTGTTGGATCAAAAGATGG+AGG - chr4.4:25885297-25885316 None:intergenic 40.0%
GTTTGTTTGTCCAACCAGTT+CGG + chr4.4:25885866-25885885 MS.gene047802:CDS 40.0%
TAATCATGGGTGAGAGATCA+AGG - chr4.4:25885224-25885243 None:intergenic 40.0%
TATAGGAAGAGGATGTTCTG+GGG - chr4.4:25885553-25885572 None:intergenic 40.0%
TCCTTTCCAGGATTCAAAAG+CGG - chr4.4:25885903-25885922 None:intergenic 40.0%
TCTTCGTATGCTATAGGAAG+AGG - chr4.4:25885564-25885583 None:intergenic 40.0%
TTCATGGACTTCCCTCAAAT+GGG + chr4.4:25885584-25885603 MS.gene047802:CDS 40.0%
TTGAGGAAATTGTGGTATGG+TGG - chr4.4:25885477-25885496 None:intergenic 40.0%
TTTGTTTGTCCAACCAGTTC+GGG + chr4.4:25885867-25885886 MS.gene047802:CDS 40.0%
! AAAAGATGGAGGTACGATGT+TGG - chr4.4:25885286-25885305 None:intergenic 40.0%
! AAACAAGGAGAGGGAGTTTT+TGG - chr4.4:25885413-25885432 None:intergenic 40.0%
! AATTGGTTATGGTGGTGTTG+TGG + chr4.4:25886025-25886044 MS.gene047802:CDS 40.0%
! CAATGGTGTTGAGGAAATTG+TGG - chr4.4:25885485-25885504 None:intergenic 40.0%
!! GGACAGAGGTTGGTATTATA+AGG + chr4.4:25885989-25886008 MS.gene047802:CDS 40.0%
!! GTTGGGTTGTAAGAGAGTTT+TGG + chr4.4:25885938-25885957 MS.gene047802:CDS 40.0%
!!! CATTCTTTTGGGGTGTTGAA+AGG + chr4.4:25885784-25885803 MS.gene047802:CDS 40.0%
AATGAGAACCGACCCATTTG+AGG - chr4.4:25885599-25885618 None:intergenic 45.0%
AGGATTCAAAAGCGGGTCAT+CGG - chr4.4:25885895-25885914 None:intergenic 45.0%
ATGAGAACCGACCCATTTGA+GGG - chr4.4:25885598-25885617 None:intergenic 45.0%
ATTCGTTGAAGGACAGAGGT+TGG + chr4.4:25885979-25885998 MS.gene047802:CDS 45.0%
CATCACATGGCTATTCGTGT+TGG + chr4.4:25885711-25885730 MS.gene047802:CDS 45.0%
CCTTTCCAGGATTCAAAAGC+GGG - chr4.4:25885902-25885921 None:intergenic 45.0%
CGTGGAAGTAAACAAGGAGA+GGG - chr4.4:25885422-25885441 None:intergenic 45.0%
GCTATAGGAAGAGGATGTTC+TGG - chr4.4:25885555-25885574 None:intergenic 45.0%
GCTATTCGTGTTGGAACTGA+AGG + chr4.4:25885720-25885739 MS.gene047802:CDS 45.0%
GGATAAGAATTTGGGGAGGT+TGG + chr4.4:25885920-25885939 MS.gene047802:CDS 45.0%
TGCCGAGAATGATTCGTTGA+AGG + chr4.4:25885968-25885987 MS.gene047802:CDS 45.0%
TGTTGAAAGGGTTGGATCTG+AGG + chr4.4:25885797-25885816 MS.gene047802:CDS 45.0%
TTGGAACTGAAGGGCTTCAA+GGG + chr4.4:25885730-25885749 MS.gene047802:CDS 45.0%
! CCTCTCCTTGTTTACTTCCA+CGG + chr4.4:25885420-25885439 MS.gene047802:CDS 45.0%
! TGGGTCGGTTCTCATTTACA+TGG + chr4.4:25885603-25885622 MS.gene047802:CDS 45.0%
! TTGTGGTATGGTGGTGAGAA+AGG - chr4.4:25885468-25885487 None:intergenic 45.0%
!!! AGGAGAGGGAGTTTTTGGTT+TGG - chr4.4:25885408-25885427 None:intergenic 45.0%
!!! TTTTGGGGTGTTGAAAGGGT+TGG + chr4.4:25885789-25885808 MS.gene047802:CDS 45.0%
CACCACCGTGGAAGTAAACA+AGG - chr4.4:25885428-25885447 None:intergenic 50.0%
CCGTGGAAGTAAACAAGGAG+AGG - chr4.4:25885423-25885442 None:intergenic 50.0%
CTTGTTTACTTCCACGGTGG+TGG + chr4.4:25885426-25885445 MS.gene047802:CDS 50.0%
GTTGGAACTGAAGGGCTTCA+AGG + chr4.4:25885729-25885748 MS.gene047802:CDS 50.0%
TGGACTTCCCTCAAATGGGT+CGG + chr4.4:25885588-25885607 MS.gene047802:CDS 50.0%
TTCTTTGCAGGAGACAGTGC+TGG + chr4.4:25885675-25885694 MS.gene047802:CDS 50.0%
! CTCCTTGTTTACTTCCACGG+TGG + chr4.4:25885423-25885442 MS.gene047802:CDS 50.0%
!! CCCGCTTTTGAATCCTGGAA+AGG + chr4.4:25885899-25885918 MS.gene047802:CDS 50.0%
!! GATGACCCGCTTTTGAATCC+TGG + chr4.4:25885894-25885913 MS.gene047802:CDS 50.0%
TCATCGGATCCCGAACTGGT+TGG - chr4.4:25885879-25885898 None:intergenic 55.0%
CTACGCAGAAACCACCACCG+TGG - chr4.4:25885440-25885459 None:intergenic 60.0%
CGGGTCATCGGATCCCGAAC+TGG - chr4.4:25885883-25885902 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 25885195 25886160 25885195 ID=MS.gene047802
chr4.4 mRNA 25885195 25886160 25885195 ID=MS.gene047802.t1;Parent=MS.gene047802
chr4.4 exon 25885195 25886160 25885195 ID=MS.gene047802.t1.exon1;Parent=MS.gene047802.t1
chr4.4 CDS 25885195 25886160 25885195 ID=cds.MS.gene047802.t1;Parent=MS.gene047802.t1
Gene Sequence

>MS.gene047802

ATGGATTCAAGCTCTAACGAAGTAGTCCTTGATCTCTCACCCATGATTAAAATCTACAAAGATGGTCACATAGAAAGACTCATAGGATCCAACATCGTACCTCCATCTTTTGATCCAACAACAAACGTAGAATCCAAAGACATTCTCATCTCGAAAGACCAAAACATATCCGTCAGAATCTTCATTCCCAAACCCAACAACGATCAATTTCCAAACCAAAAACTCCCTCTCCTTGTTTACTTCCACGGTGGTGGTTTCTGCGTAGAAACACCTTTCTCACCACCATACCACAATTTCCTCAACACCATTGTTTCTAAAGCTAATGTGATCGCTGTTTCAGTTGATTACAGAAGAGCCCCAGAACATCCTCTTCCTATAGCATACGAAGATTCATGGACTTCCCTCAAATGGGTCGGTTCTCATTTACATGGAAATGGTTCTGATGAATGGATCAACCGTTATGCAGATTTTGGAAAAGTGTTCTTTGCAGGAGACAGTGCTGGAGCAAACATTGCACATCACATGGCTATTCGTGTTGGAACTGAAGGGCTTCAAGGGATTAACCTTGAAGGGATTGTTTTGGTTCATACATTCTTTTGGGGTGTTGAAAGGGTTGGATCTGAGGCTGCTGAGAAATCTGAACATTTATCTTTGGCAGATAATTTATGGAGGTTTGTTTGTCCAACCAGTTCGGGATCCGATGACCCGCTTTTGAATCCTGGAAAGGATAAGAATTTGGGGAGGTTGGGTTGTAAGAGAGTTTTGGTTTGTGTTGCCGAGAATGATTCGTTGAAGGACAGAGGTTGGTATTATAAGGAGTTGCTTGAGAAAATTGGTTATGGTGGTGTTGTGGAAGTGATTGAGACAAAAGGTGAAGGACATGTTTTTCATCTCTTTAATCCAAATTGTGATAACGCTACTTCTTTGCTTAATCAAATTGCTTCCTTTATCAAGCATAGTGGTTAA
Protein sequence

>MS.gene047802.t1

MDSSSNEVVLDLSPMIKIYKDGHIERLIGSNIVPPSFDPTTNVESKDILISKDQNISVRIFIPKPNNDQFPNQKLPLLVYFHGGGFCVETPFSPPYHNFLNTIVSKANVIAVSVDYRRAPEHPLPIAYEDSWTSLKWVGSHLHGNGSDEWINRYADFGKVFFAGDSAGANIAHHMAIRVGTEGLQGINLEGIVLVHTFFWGVERVGSEAAEKSEHLSLADNLWRFVCPTSSGSDDPLLNPGKDKNLGRLGCKRVLVCVAENDSLKDRGWYYKELLEKIGYGGVVEVIETKGEGHVFHLFNPNCDNATSLLNQIASFIKHSG