Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29383.t1 | KEH35030.1 | 99.6 | 264 | 1 | 0 | 1 | 264 | 1 | 264 | 5.90E-119 | 437.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29383.t1 | Q9ZSZ8 | 69.5 | 259 | 66 | 3 | 10 | 264 | 7 | 256 | 8.4e-66 | 252.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29383.t1 | A0A072VA17 | 99.6 | 264 | 1 | 0 | 1 | 264 | 1 | 264 | 4.2e-119 | 437.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene29383 | 0.816579 | 5.14E-52 | -1.69E-46 |
| MS.gene049972 | MS.gene29383 | 0.827554 | 1.47E-54 | -1.69E-46 |
| MS.gene04998 | MS.gene29383 | 0.809486 | 1.85E-50 | -1.69E-46 |
| MS.gene050838 | MS.gene29383 | 0.800603 | 1.33E-48 | -1.69E-46 |
| MS.gene050945 | MS.gene29383 | 0.818265 | 2.15E-52 | -1.69E-46 |
| MS.gene051003 | MS.gene29383 | 0.808703 | 2.72E-50 | -1.69E-46 |
| MS.gene051676 | MS.gene29383 | 0.813938 | 1.99E-51 | -1.69E-46 |
| MS.gene051699 | MS.gene29383 | 0.819768 | 9.77E-53 | -1.69E-46 |
| MS.gene051902 | MS.gene29383 | 0.833326 | 5.69E-56 | -1.69E-46 |
| MS.gene051975 | MS.gene29383 | 0.809815 | 1.57E-50 | -1.69E-46 |
| MS.gene052072 | MS.gene29383 | 0.823861 | 1.10E-53 | -1.69E-46 |
| MS.gene052296 | MS.gene29383 | 0.804743 | 1.87E-49 | -1.69E-46 |
| MS.gene052342 | MS.gene29383 | 0.80753 | 4.83E-50 | -1.69E-46 |
| MS.gene052533 | MS.gene29383 | 0.800807 | 1.21E-48 | -1.69E-46 |
| MS.gene052818 | MS.gene29383 | -0.814498 | 1.49E-51 | -1.69E-46 |
| MS.gene052947 | MS.gene29383 | 0.802654 | 5.06E-49 | -1.69E-46 |
| MS.gene053298 | MS.gene29383 | 0.818224 | 2.19E-52 | -1.69E-46 |
| MS.gene053963 | MS.gene29383 | 0.811713 | 6.10E-51 | -1.69E-46 |
| MS.gene054222 | MS.gene29383 | 0.833433 | 5.35E-56 | -1.69E-46 |
| MS.gene055555 | MS.gene29383 | 0.805176 | 1.51E-49 | -1.69E-46 |
| MS.gene05636 | MS.gene29383 | 0.807581 | 4.71E-50 | -1.69E-46 |
| MS.gene056638 | MS.gene29383 | 0.810515 | 1.11E-50 | -1.69E-46 |
| MS.gene056986 | MS.gene29383 | 0.829084 | 6.27E-55 | -1.69E-46 |
| MS.gene057102 | MS.gene29383 | 0.852889 | 3.43E-61 | -1.69E-46 |
| MS.gene057576 | MS.gene29383 | 0.836832 | 7.42E-57 | -1.69E-46 |
| MS.gene057584 | MS.gene29383 | 0.843668 | 1.22E-58 | -1.69E-46 |
| MS.gene057586 | MS.gene29383 | 0.825468 | 4.61E-54 | -1.69E-46 |
| MS.gene057588 | MS.gene29383 | 0.835269 | 1.85E-56 | -1.69E-46 |
| MS.gene057890 | MS.gene29383 | -0.821089 | 4.86E-53 | -1.69E-46 |
| MS.gene057999 | MS.gene29383 | 0.825218 | 5.28E-54 | -1.69E-46 |
| MS.gene058074 | MS.gene29383 | 0.854462 | 1.21E-61 | -1.69E-46 |
| MS.gene058159 | MS.gene29383 | 0.811247 | 7.70E-51 | -1.69E-46 |
| MS.gene059146 | MS.gene29383 | 0.848976 | 4.35E-60 | -1.69E-46 |
| MS.gene059227 | MS.gene29383 | 0.808292 | 3.33E-50 | -1.69E-46 |
| MS.gene05985 | MS.gene29383 | -0.812435 | 4.25E-51 | -1.69E-46 |
| MS.gene06011 | MS.gene29383 | 0.832162 | 1.11E-55 | -1.69E-46 |
| MS.gene060259 | MS.gene29383 | 0.806086 | 9.76E-50 | -1.69E-46 |
| MS.gene060365 | MS.gene29383 | 0.806401 | 8.38E-50 | -1.69E-46 |
| MS.gene060763 | MS.gene29383 | 0.8079 | 4.03E-50 | -1.69E-46 |
| MS.gene060935 | MS.gene29383 | 0.804589 | 2.01E-49 | -1.69E-46 |
| MS.gene060937 | MS.gene29383 | 0.818274 | 2.14E-52 | -1.69E-46 |
| MS.gene061100 | MS.gene29383 | 0.80065 | 1.30E-48 | -1.69E-46 |
| MS.gene061160 | MS.gene29383 | 0.800085 | 1.70E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29383.t1 | MTR_3g077320 | 96.791 | 374 | 8 | 1 | 1 | 370 | 1 | 374 | 0.0 | 737 |
| MS.gene29383.t1 | MTR_3g077320 | 99.259 | 270 | 2 | 0 | 1 | 270 | 1 | 270 | 0.0 | 558 |
| MS.gene29383.t1 | MTR_0325s0020 | 68.770 | 317 | 94 | 3 | 10 | 322 | 209 | 524 | 2.51e-147 | 427 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29383.t1 | AT3G54170 | 69.097 | 288 | 74 | 4 | 10 | 292 | 7 | 284 | 1.04e-133 | 385 |
Find 93 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGACTCTTCTGATGATTTC+AGG | 0.166919 | 3.2:+68421653 | None:intergenic |
| TTCGATTTCGGAGGTGAAAT+TGG | 0.256929 | 3.2:-68424077 | MS.gene29383:CDS |
| GAAATTGGTGGAAGGCATTC+AGG | 0.288629 | 3.2:-68424062 | MS.gene29383:intron |
| CATCTTCCCCATCTTGCTTT+GGG | 0.292814 | 3.2:+68419177 | None:intergenic |
| CATTAAAACATTTGCAAAAC+GGG | 0.325057 | 3.2:-68421494 | MS.gene29383:CDS |
| GCATCTTCCCCATCTTGCTT+TGG | 0.337652 | 3.2:+68419176 | None:intergenic |
| AGTTTCTTCAACTTTAGATT+TGG | 0.349237 | 3.2:+68422508 | None:intergenic |
| AATCAATCAATTACCTTCTT+CGG | 0.359272 | 3.2:+68423905 | None:intergenic |
| GTTTCAGTCTTTGAATCTCC+TGG | 0.375507 | 3.2:+68417332 | None:intergenic |
| TAATCTTCGTCGTCGTAATC+CGG | 0.402595 | 3.2:+68423947 | None:intergenic |
| TTACATCGTCGAAACGAGTT+GGG | 0.405362 | 3.2:+68424182 | None:intergenic |
| TATAAGCATACCTGCATTGA+TGG | 0.414521 | 3.2:+68420664 | None:intergenic |
| AATGTTGATGAATTATCTTC+AGG | 0.414876 | 3.2:-68421677 | MS.gene29383:CDS |
| GTATGTGCTGGGGCTACTCC+AGG | 0.422901 | 3.2:-68421844 | MS.gene29383:intron |
| TGAAGAGTTCACACGTTTGA+AGG | 0.425230 | 3.2:-68419983 | MS.gene29383:intron |
| GGCGGTGACTGAGGAGGATA+AGG | 0.427854 | 3.2:-68417185 | MS.gene29383:CDS |
| GACGGTGGCTGAGGAGGATA+AGG | 0.427854 | 3.2:-68417215 | MS.gene29383:CDS |
| GCTTTGGGAAGTAAATGTTA+TGG | 0.432063 | 3.2:+68419192 | None:intergenic |
| CAACTCAGAAGTCAATTTGA+AGG | 0.433565 | 3.2:-68418830 | MS.gene29383:intron |
| TGACTCTTCTGATGATTTCA+GGG | 0.438756 | 3.2:+68421654 | None:intergenic |
| AAACTTTCAGTCTGCTGTGC+GGG | 0.448301 | 3.2:-68420710 | MS.gene29383:intron |
| ATTACCTTCTTCGGTGCAAA+AGG | 0.449253 | 3.2:+68423914 | None:intergenic |
| CAAGAAGAAAACGAGGAGAT+TGG | 0.450411 | 3.2:-68419130 | MS.gene29383:CDS |
| TCATTAAAACATTTGCAAAA+CGG | 0.457616 | 3.2:-68421495 | MS.gene29383:CDS |
| TACAGGGTTGCAGAAGCATA+TGG | 0.468685 | 3.2:-68418290 | MS.gene29383:intron |
| TTGCAGCCTCCAGAGCTGTA+AGG | 0.469485 | 3.2:-68422348 | MS.gene29383:intron |
| TTTACTTCCCAAAGCAAGAT+GGG | 0.471815 | 3.2:-68419184 | MS.gene29383:CDS |
| CTCATCCTATCAGAATTTAG+TGG | 0.474105 | 3.2:-68419260 | MS.gene29383:intron |
| AATGAGCCTGTTGTATGTGC+TGG | 0.474348 | 3.2:-68421856 | MS.gene29383:CDS |
| AAGGAGGTTGTTACTAGATC+CGG | 0.480093 | 3.2:-68420013 | MS.gene29383:CDS |
| TACTGGTTGTCAGCTTGAAA+AGG | 0.490273 | 3.2:-68421539 | MS.gene29383:intron |
| GTAGCCCCAGCACATACAAC+AGG | 0.493156 | 3.2:+68421850 | None:intergenic |
| CAAGCCGATCCTTACAGCTC+TGG | 0.493375 | 3.2:+68422339 | None:intergenic |
| ATGAGCCTGTTGTATGTGCT+GGG | 0.502100 | 3.2:-68421855 | MS.gene29383:CDS |
| ACTGCCTCTGGTGTAACAAC+AGG | 0.502296 | 3.2:-68422488 | MS.gene29383:CDS |
| ATTTACTTCCCAAAGCAAGA+TGG | 0.502506 | 3.2:-68419185 | MS.gene29383:CDS |
| GAAGCTAGACGAGAAAGGCC+AGG | 0.503947 | 3.2:-68417350 | MS.gene29383:CDS |
| CTTACATCGTCGAAACGAGT+TGG | 0.509237 | 3.2:+68424181 | None:intergenic |
| AGAAGAGTCATTGAGAGAGC+AGG | 0.511779 | 3.2:-68421641 | MS.gene29383:intron |
| GGATTTGCAAGATAACATTA+CGG | 0.511867 | 3.2:-68419215 | MS.gene29383:CDS |
| TGAGGAGGATAAGGAGGATA+AGG | 0.515579 | 3.2:-68417206 | MS.gene29383:CDS |
| CAAAAGAACTTAGCGGAGGA+TGG | 0.520918 | 3.2:-68417298 | MS.gene29383:CDS |
| CAGTGATAAGACGGTGGCTG+AGG | 0.530018 | 3.2:-68417245 | MS.gene29383:CDS |
| AAATTAATTCGCAGCCTCCT+CGG | 0.535628 | 3.2:+68417135 | None:intergenic |
| CAAGAGAAGCTAGACGAGAA+AGG | 0.541633 | 3.2:-68417355 | MS.gene29383:CDS |
| AAAGTTGAAGAAACTGCCTC+TGG | 0.544941 | 3.2:-68422500 | MS.gene29383:CDS |
| AGAACTTAGCGGAGGATGGA+AGG | 0.546938 | 3.2:-68417294 | MS.gene29383:CDS |
| GGAGGATAAGACGGTGGCTG+AGG | 0.547486 | 3.2:-68417224 | MS.gene29383:CDS |
| ACTCAAGCCGCCATCAATGC+AGG | 0.552351 | 3.2:-68420674 | MS.gene29383:intron |
| TGATAAGACGGTGGCTGAGG+AGG | 0.559728 | 3.2:-68417242 | MS.gene29383:CDS |
| GGATAAGACGGTGGCTGAGG+AGG | 0.560217 | 3.2:-68417221 | MS.gene29383:CDS |
| CAAAGTCTGAGATTCATAGT+TGG | 0.562090 | 3.2:-68421894 | MS.gene29383:CDS |
| TCAGAATTTAGTGGAGGAGA+AGG | 0.567099 | 3.2:-68419251 | MS.gene29383:intron |
| GATTTCGGAGGTGAAATTGG+TGG | 0.567451 | 3.2:-68424074 | MS.gene29383:CDS |
| GTGAACTCTTCATGAACAGC+CGG | 0.571799 | 3.2:+68419994 | None:intergenic |
| TCGTCGTCGTAATCCGGCGA+TGG | 0.576592 | 3.2:+68423953 | None:intergenic |
| AAATCAAGCTTCTGAAGGAA+AGG | 0.576681 | 3.2:-68419107 | MS.gene29383:intron |
| GCCTCCAGAGCTGTAAGGAT+CGG | 0.577211 | 3.2:-68422343 | MS.gene29383:CDS |
| TGTGGAGAGATCAAATGAAA+CGG | 0.578417 | 3.2:-68418251 | MS.gene29383:intron |
| GTATTTCCAGAGTGAGCTTG+AGG | 0.596741 | 3.2:-68421920 | MS.gene29383:intron |
| GCCGATCCTTACAGCTCTGG+AGG | 0.599157 | 3.2:+68422342 | None:intergenic |
| GGAGGATAAGGCGGTGACTG+AGG | 0.608632 | 3.2:-68417194 | MS.gene29383:CDS |
| TCGGAGGTGAAATTGGTGGA+AGG | 0.608878 | 3.2:-68424070 | MS.gene29383:CDS |
| ACTCCAGCAAAAGAACTTAG+CGG | 0.619444 | 3.2:-68417305 | MS.gene29383:CDS |
| TACATCGTCGAAACGAGTTG+GGG | 0.620919 | 3.2:+68424183 | None:intergenic |
| TAAGAGTGACAGTGATAAGA+CGG | 0.623426 | 3.2:-68417254 | MS.gene29383:CDS |
| ATTGGAAATCAAGCTTCTGA+AGG | 0.624930 | 3.2:-68419112 | MS.gene29383:CDS |
| GGAGGATAAGGCGGTGACTG+AGG | 0.626582 | 3.2:-68417173 | MS.gene29383:CDS |
| GGATAAGGCGGTGACTGAGG+AGG | 0.629527 | 3.2:-68417170 | MS.gene29383:CDS |
| GGATAAGGCGGTGACTGAGG+AGG | 0.629527 | 3.2:-68417191 | MS.gene29383:CDS |
| CATTCCTGTTGTTACACCAG+AGG | 0.631750 | 3.2:+68422484 | None:intergenic |
| GGTGGCTGAGGAGGATAAGA+CGG | 0.632347 | 3.2:-68417233 | MS.gene29383:CDS |
| TCACTGTCACTCTTACTATG+TGG | 0.638863 | 3.2:+68417262 | None:intergenic |
| GGTGGCTGAGGAGGATAAGG+AGG | 0.640276 | 3.2:-68417212 | MS.gene29383:CDS |
| GGAGGATAAGACAGTGACCG+AGG | 0.642486 | 3.2:-68417152 | MS.gene29383:CDS |
| TGATCTTGTCACTTCGTGAG+AGG | 0.642970 | 3.2:-68422463 | MS.gene29383:intron |
| CAAGATGGGGAAGATGCTGA+TGG | 0.643010 | 3.2:-68419170 | MS.gene29383:CDS |
| GAAACTTTCAGTCTGCTGTG+CGG | 0.644244 | 3.2:-68420711 | MS.gene29383:intron |
| AAGCATACCTGCATTGATGG+CGG | 0.659147 | 3.2:+68420667 | None:intergenic |
| GGGTTGCAGAAGCATATGGA+AGG | 0.661064 | 3.2:-68418286 | MS.gene29383:CDS |
| ATCCTATCAGAATTTAGTGG+AGG | 0.662269 | 3.2:-68419257 | MS.gene29383:intron |
| AAAACGGGAGCAAGAGATAG+CGG | 0.663083 | 3.2:-68421479 | MS.gene29383:CDS |
| TTACTTCCCAAAGCAAGATG+GGG | 0.664236 | 3.2:-68419183 | MS.gene29383:CDS |
| GGAAGGACTGACAAATGATG+TGG | 0.666612 | 3.2:-68418269 | MS.gene29383:CDS |
| GGATAAGACAGTGACCGAGG+AGG | 0.668143 | 3.2:-68417149 | MS.gene29383:CDS |
| AGGTAATAAGAGACCATCGC+CGG | 0.668265 | 3.2:-68423966 | MS.gene29383:CDS |
| AACCCTGCAAGAAGAAAACG+AGG | 0.669091 | 3.2:-68419137 | MS.gene29383:CDS |
| CCAGCAAAAGAACTTAGCGG+AGG | 0.696105 | 3.2:-68417302 | MS.gene29383:CDS |
| GGAGGATAAGGAGGATAAGG+CGG | 0.697058 | 3.2:-68417203 | MS.gene29383:CDS |
| GGTGACTGAGGAGGATAAGG+CGG | 0.699747 | 3.2:-68417182 | MS.gene29383:CDS |
| GGCTGAGGAGGATAAGACGG+TGG | 0.703854 | 3.2:-68417230 | MS.gene29383:CDS |
| TGAGCCTGTTGTATGTGCTG+GGG | 0.705363 | 3.2:-68421854 | MS.gene29383:CDS |
| GAGTGACAGTGATAAGACGG+TGG | 0.746274 | 3.2:-68417251 | MS.gene29383:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 68417138 | 68424212 | 68417138 | ID=MS.gene29383 |
| chr3.2 | mRNA | 68417138 | 68424212 | 68417138 | ID=MS.gene29383.t1;Parent=MS.gene29383 |
| chr3.2 | exon | 68424186 | 68424212 | 68424186 | ID=MS.gene29383.t1.exon1;Parent=MS.gene29383.t1 |
| chr3.2 | CDS | 68424186 | 68424212 | 68424186 | ID=cds.MS.gene29383.t1;Parent=MS.gene29383.t1 |
| chr3.2 | exon | 68424063 | 68424102 | 68424063 | ID=MS.gene29383.t1.exon2;Parent=MS.gene29383.t1 |
| chr3.2 | CDS | 68424063 | 68424102 | 68424063 | ID=cds.MS.gene29383.t1;Parent=MS.gene29383.t1 |
| chr3.2 | exon | 68423919 | 68423986 | 68423919 | ID=MS.gene29383.t1.exon3;Parent=MS.gene29383.t1 |
| chr3.2 | CDS | 68423919 | 68423986 | 68423919 | ID=cds.MS.gene29383.t1;Parent=MS.gene29383.t1 |
| chr3.2 | exon | 68422464 | 68422531 | 68422464 | ID=MS.gene29383.t1.exon4;Parent=MS.gene29383.t1 |
| chr3.2 | CDS | 68422464 | 68422531 | 68422464 | ID=cds.MS.gene29383.t1;Parent=MS.gene29383.t1 |
| chr3.2 | exon | 68422328 | 68422364 | 68422328 | ID=MS.gene29383.t1.exon5;Parent=MS.gene29383.t1 |
| chr3.2 | CDS | 68422328 | 68422364 | 68422328 | ID=cds.MS.gene29383.t1;Parent=MS.gene29383.t1 |
| chr3.2 | exon | 68421845 | 68421932 | 68421845 | ID=MS.gene29383.t1.exon6;Parent=MS.gene29383.t1 |
| chr3.2 | CDS | 68421845 | 68421932 | 68421845 | ID=cds.MS.gene29383.t1;Parent=MS.gene29383.t1 |
| chr3.2 | exon | 68421642 | 68421706 | 68421642 | ID=MS.gene29383.t1.exon7;Parent=MS.gene29383.t1 |
| chr3.2 | CDS | 68421642 | 68421706 | 68421642 | ID=cds.MS.gene29383.t1;Parent=MS.gene29383.t1 |
| chr3.2 | exon | 68421471 | 68421548 | 68421471 | ID=MS.gene29383.t1.exon8;Parent=MS.gene29383.t1 |
| chr3.2 | CDS | 68421471 | 68421548 | 68421471 | ID=cds.MS.gene29383.t1;Parent=MS.gene29383.t1 |
| chr3.2 | exon | 68420675 | 68420722 | 68420675 | ID=MS.gene29383.t1.exon9;Parent=MS.gene29383.t1 |
| chr3.2 | CDS | 68420675 | 68420722 | 68420675 | ID=cds.MS.gene29383.t1;Parent=MS.gene29383.t1 |
| chr3.2 | exon | 68419984 | 68420037 | 68419984 | ID=MS.gene29383.t1.exon10;Parent=MS.gene29383.t1 |
| chr3.2 | CDS | 68419984 | 68420037 | 68419984 | ID=cds.MS.gene29383.t1;Parent=MS.gene29383.t1 |
| chr3.2 | exon | 68419108 | 68419269 | 68419108 | ID=MS.gene29383.t1.exon11;Parent=MS.gene29383.t1 |
| chr3.2 | CDS | 68419108 | 68419269 | 68419108 | ID=cds.MS.gene29383.t1;Parent=MS.gene29383.t1 |
| chr3.2 | exon | 68418831 | 68418900 | 68418831 | ID=MS.gene29383.t1.exon12;Parent=MS.gene29383.t1 |
| chr3.2 | CDS | 68418831 | 68418900 | 68418831 | ID=cds.MS.gene29383.t1;Parent=MS.gene29383.t1 |
| chr3.2 | exon | 68418252 | 68418307 | 68418252 | ID=MS.gene29383.t1.exon13;Parent=MS.gene29383.t1 |
| chr3.2 | CDS | 68418252 | 68418307 | 68418252 | ID=cds.MS.gene29383.t1;Parent=MS.gene29383.t1 |
| chr3.2 | exon | 68417138 | 68417389 | 68417138 | ID=MS.gene29383.t1.exon14;Parent=MS.gene29383.t1 |
| chr3.2 | CDS | 68417138 | 68417389 | 68417138 | ID=cds.MS.gene29383.t1;Parent=MS.gene29383.t1 |
| Gene Sequence |
| Protein sequence |
