Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30663.t1 | RHN51488.1 | 97.4 | 154 | 4 | 0 | 1 | 154 | 173 | 326 | 4.30E-84 | 320.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30663.t1 | Q84WV9 | 60.5 | 147 | 57 | 1 | 1 | 147 | 174 | 319 | 5.4e-51 | 201.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30663.t1 | A0A072TTV2 | 97.4 | 154 | 4 | 0 | 1 | 154 | 172 | 325 | 3.1e-84 | 320.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene30663 | 0.813131 | 2.99E-51 | -1.69E-46 |
| MS.gene04918 | MS.gene30663 | 0.800846 | 1.19E-48 | -1.69E-46 |
| MS.gene049786 | MS.gene30663 | 0.800403 | 1.47E-48 | -1.69E-46 |
| MS.gene049825 | MS.gene30663 | 0.822608 | 2.16E-53 | -1.69E-46 |
| MS.gene050253 | MS.gene30663 | 0.801685 | 8.01E-49 | -1.69E-46 |
| MS.gene051247 | MS.gene30663 | 0.802233 | 6.18E-49 | -1.69E-46 |
| MS.gene051700 | MS.gene30663 | 0.827587 | 1.44E-54 | -1.69E-46 |
| MS.gene05188 | MS.gene30663 | 0.815106 | 1.10E-51 | -1.69E-46 |
| MS.gene051902 | MS.gene30663 | 0.8139 | 2.03E-51 | -1.69E-46 |
| MS.gene051975 | MS.gene30663 | 0.809471 | 1.86E-50 | -1.69E-46 |
| MS.gene052463 | MS.gene30663 | 0.81666 | 4.93E-52 | -1.69E-46 |
| MS.gene052519 | MS.gene30663 | 0.824347 | 8.48E-54 | -1.69E-46 |
| MS.gene052685 | MS.gene30663 | 0.81008 | 1.38E-50 | -1.69E-46 |
| MS.gene052889 | MS.gene30663 | 0.823492 | 1.35E-53 | -1.69E-46 |
| MS.gene053298 | MS.gene30663 | 0.828814 | 7.29E-55 | -1.69E-46 |
| MS.gene053677 | MS.gene30663 | 0.80865 | 2.79E-50 | -1.69E-46 |
| MS.gene053786 | MS.gene30663 | 0.832135 | 1.12E-55 | -1.69E-46 |
| MS.gene053963 | MS.gene30663 | 0.8231 | 1.66E-53 | -1.69E-46 |
| MS.gene054222 | MS.gene30663 | 0.810552 | 1.09E-50 | -1.69E-46 |
| MS.gene05458 | MS.gene30663 | 0.821418 | 4.08E-53 | -1.69E-46 |
| MS.gene054651 | MS.gene30663 | 0.800273 | 1.56E-48 | -1.69E-46 |
| MS.gene055513 | MS.gene30663 | 0.835447 | 1.67E-56 | -1.69E-46 |
| MS.gene055603 | MS.gene30663 | 0.831276 | 1.83E-55 | -1.69E-46 |
| MS.gene05636 | MS.gene30663 | 0.812739 | 3.64E-51 | -1.69E-46 |
| MS.gene056403 | MS.gene30663 | 0.817771 | 2.77E-52 | -1.69E-46 |
| MS.gene056638 | MS.gene30663 | 0.813936 | 1.99E-51 | -1.69E-46 |
| MS.gene056699 | MS.gene30663 | 0.80922 | 2.11E-50 | -1.69E-46 |
| MS.gene056986 | MS.gene30663 | 0.830837 | 2.34E-55 | -1.69E-46 |
| MS.gene057102 | MS.gene30663 | 0.856601 | 2.88E-62 | -1.69E-46 |
| MS.gene058159 | MS.gene30663 | 0.806996 | 6.27E-50 | -1.69E-46 |
| MS.gene059774 | MS.gene30663 | 0.801823 | 7.51E-49 | -1.69E-46 |
| MS.gene05985 | MS.gene30663 | -0.807516 | 4.87E-50 | -1.69E-46 |
| MS.gene06011 | MS.gene30663 | 0.844856 | 5.83E-59 | -1.69E-46 |
| MS.gene060572 | MS.gene30663 | 0.802285 | 6.03E-49 | -1.69E-46 |
| MS.gene060937 | MS.gene30663 | 0.800818 | 1.21E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30663.t1 | MTR_0057s0080 | 97.403 | 154 | 4 | 0 | 1 | 154 | 172 | 325 | 9.87e-111 | 317 |
| MS.gene30663.t1 | MTR_0057s0130 | 89.610 | 154 | 16 | 0 | 1 | 154 | 130 | 283 | 3.28e-98 | 284 |
| MS.gene30663.t1 | MTR_0057s0130 | 90.517 | 116 | 11 | 0 | 1 | 116 | 130 | 245 | 1.36e-72 | 218 |
| MS.gene30663.t1 | MTR_4g012780 | 100.000 | 32 | 0 | 0 | 1 | 32 | 77 | 108 | 2.12e-15 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30663.t1 | AT4G25720 | 60.544 | 147 | 57 | 1 | 1 | 147 | 174 | 319 | 2.54e-63 | 197 |
| MS.gene30663.t1 | AT4G25720 | 64.655 | 116 | 41 | 0 | 1 | 116 | 174 | 289 | 8.35e-53 | 169 |
| MS.gene30663.t1 | AT4G25720 | 50.340 | 147 | 52 | 2 | 1 | 147 | 174 | 299 | 8.31e-48 | 156 |
| MS.gene30663.t1 | AT4G25720 | 64.948 | 97 | 34 | 0 | 1 | 97 | 174 | 270 | 2.59e-42 | 142 |
| MS.gene30663.t1 | AT4G25720 | 71.429 | 63 | 18 | 0 | 1 | 63 | 174 | 236 | 6.90e-27 | 101 |
Find 0 sgRNAs with CRISPR-Local
Find 110 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAATATTTGTACAAAATA+AGG | - | 42997:14541-14560 | None:intergenic | 10.0% |
| !! | TTTATAATAAATTGAAAAAC+TGG | - | 42997:14071-14090 | None:intergenic | 10.0% |
| !! | ATGATACATTTAAATAGAAA+AGG | - | 42997:14106-14125 | None:intergenic | 15.0% |
| !! | CATAAACTTAATAAACTTAA+TGG | - | 42997:14624-14643 | None:intergenic | 15.0% |
| !! | TATTATTTGTAGATATCAAA+AGG | - | 42997:13768-13787 | None:intergenic | 15.0% |
| !!! | TGTATTGGTTATATTTTATA+AGG | + | 42997:13802-13821 | MS.gene30663:intron | 15.0% |
| !! | AAAACAAGTTATCTACTATA+AGG | + | 42997:13132-13151 | MS.gene30663:CDS | 20.0% |
| !! | AAACAAGTTATCTACTATAA+GGG | + | 42997:13133-13152 | MS.gene30663:CDS | 20.0% |
| !! | AATATATAAATGGTGAAGTT+TGG | + | 42997:13185-13204 | MS.gene30663:CDS | 20.0% |
| !! | AATGAACTGGAATATATAAA+TGG | + | 42997:13175-13194 | MS.gene30663:CDS | 20.0% |
| !! | ATATATAAATGGTGAAGTTT+GGG | + | 42997:13186-13205 | MS.gene30663:CDS | 20.0% |
| !! | ATCATCAAAAAATGTTTCAT+AGG | - | 42997:13593-13612 | None:intergenic | 20.0% |
| !! | GATATCTACAAATAATACTT+TGG | + | 42997:13771-13790 | MS.gene30663:intron | 20.0% |
| !! | GTAGATTTACAACATTAAAT+TGG | + | 42997:13526-13545 | MS.gene30663:intron | 20.0% |
| !! | TTAAAAAGCTAGAAACAAAA+GGG | - | 42997:13032-13051 | None:intergenic | 20.0% |
| !!! | GAGATCAAAATTGTTATTTA+AGG | + | 42997:13934-13953 | MS.gene30663:intron | 20.0% |
| !!! | GTGTTGATCTTTTATATTTT+AGG | + | 42997:13984-14003 | MS.gene30663:intron | 20.0% |
| !!! | TTGTTTTACCATTTTATAAG+CGG | + | 42997:12991-13010 | MS.gene30663:intron | 20.0% |
| !!! | TTTAGTGGATATTTTTAAGA+AGG | - | 42997:13450-13469 | None:intergenic | 20.0% |
| ! | AAACATAAGCAATGTGAAAA+AGG | + | 42997:14304-14323 | MS.gene30663:intron | 25.0% |
| ! | AGAAAAATGTTATCCATGTA+TGG | + | 42997:13270-13289 | MS.gene30663:intron | 25.0% |
| ! | ATTGATCCTCAAACATTTAA+AGG | + | 42997:12939-12958 | MS.gene30663:CDS | 25.0% |
| ! | GAAGATAAACTAAACAGTAT+TGG | - | 42997:13660-13679 | None:intergenic | 25.0% |
| ! | GTTAAAAAGCTAGAAACAAA+AGG | - | 42997:13033-13052 | None:intergenic | 25.0% |
| ! | TAACCGTAAGAAACAAATAA+AGG | - | 42997:13682-13701 | None:intergenic | 25.0% |
| ! | TGAAAATAAGGGTCATATTA+AGG | + | 42997:14179-14198 | MS.gene30663:intron | 25.0% |
| ! | TGATGCCAAAAATTTATCAT+TGG | + | 42997:13615-13634 | MS.gene30663:intron | 25.0% |
| !! | ACTTTGATTTCAAACAACTT+TGG | - | 42997:14686-14705 | None:intergenic | 25.0% |
| !! | ATGATTTTAAATCACATGCA+AGG | + | 42997:13555-13574 | MS.gene30663:intron | 25.0% |
| !! | CATGTGATTTAAAATCATTG+GGG | - | 42997:13553-13572 | None:intergenic | 25.0% |
| !! | CATTTTTCTTCACGTTTTTA+AGG | - | 42997:13259-13278 | None:intergenic | 25.0% |
| !! | CTTCCTTTATTTGTTTCTTA+CGG | + | 42997:13676-13695 | MS.gene30663:intron | 25.0% |
| !! | GCATGTGATTTAAAATCATT+GGG | - | 42997:13554-13573 | None:intergenic | 25.0% |
| !! | TAAGGCAATGAGTTTAAAAT+CGG | + | 42997:13820-13839 | MS.gene30663:intron | 25.0% |
| !! | TGCATGTGATTTAAAATCAT+TGG | - | 42997:13555-13574 | None:intergenic | 25.0% |
| !!! | AGGATATTGATGTTTTGAAT+GGG | + | 42997:14004-14023 | MS.gene30663:intron | 25.0% |
| !!! | AGTTTTTTTTCAAACCAATC+TGG | + | 42997:14582-14601 | MS.gene30663:intron | 25.0% |
| !!! | CTCTAAAATACTGAATACAA+TGG | + | 42997:14244-14263 | MS.gene30663:intron | 25.0% |
| !!! | GTGGTATTTTTGTAAGTAAA+GGG | + | 42997:14326-14345 | MS.gene30663:intron | 25.0% |
| !!! | TAGGATATTGATGTTTTGAA+TGG | + | 42997:14003-14022 | MS.gene30663:intron | 25.0% |
| AAAAGAAAACTAAGCCAGAT+TGG | - | 42997:14599-14618 | None:intergenic | 30.0% | |
| AAGAGTTTCCGCTTATAAAA+TGG | - | 42997:13002-13021 | None:intergenic | 30.0% | |
| AATTCTTTCCTGCATAGAAA+AGG | - | 42997:13712-13731 | None:intergenic | 30.0% | |
| AGATGTCACACCTTAAATTT+GGG | - | 42997:13422-13441 | None:intergenic | 30.0% | |
| ATAAAGTCAAGAATTGAGGA+AGG | + | 42997:14713-14732 | MS.gene30663:CDS | 30.0% | |
| ATAACACGCGATGAAAATAA+GGG | + | 42997:14168-14187 | MS.gene30663:intron | 30.0% | |
| ATCTACTGAAGAACCAAATT+CGG | - | 42997:13512-13531 | None:intergenic | 30.0% | |
| GCCAAAAATTTATCATTGGA+GGG | + | 42997:13619-13638 | MS.gene30663:intron | 30.0% | |
| TAAATGCTTGTACGTATGTA+TGG | + | 42997:13466-13485 | MS.gene30663:intron | 30.0% | |
| TAAGTCTTGAATTGACAGAA+CGG | - | 42997:14131-14150 | None:intergenic | 30.0% | |
| TCCTCAATTCTTGACTTTAT+GGG | - | 42997:14713-14732 | None:intergenic | 30.0% | |
| TCTCGAAACAGGAATATATA+AGG | - | 42997:13918-13937 | None:intergenic | 30.0% | |
| TGCCAAAAATTTATCATTGG+AGG | + | 42997:13618-13637 | MS.gene30663:intron | 30.0% | |
| TTAAACATCTGTGTAGGAAA+CGG | + | 42997:14495-14514 | MS.gene30663:intron | 30.0% | |
| TTAATCCAATGCCTGAAAAA+AGG | + | 42997:14364-14383 | MS.gene30663:intron | 30.0% | |
| TTCCTCAATTCTTGACTTTA+TGG | - | 42997:14714-14733 | None:intergenic | 30.0% | |
| ! | ATGTTTAACCTTTTCTATGC+AGG | + | 42997:13701-13720 | MS.gene30663:intron | 30.0% |
| ! | GTTTATGTGACTTTTGTTCT+TGG | + | 42997:14637-14656 | MS.gene30663:intron | 30.0% |
| ! | TCCCTCCAATGATAAATTTT+TGG | - | 42997:13623-13642 | None:intergenic | 30.0% |
| !! | AACAATTTTGATCTCGAAAC+AGG | - | 42997:13929-13948 | None:intergenic | 30.0% |
| !! | ACTTTGGTCTTATTGTGTAT+TGG | + | 42997:13787-13806 | MS.gene30663:intron | 30.0% |
| !! | AGTTTGGGCAAATGTTTTTA+CGG | + | 42997:13201-13220 | MS.gene30663:CDS | 30.0% |
| !! | CCTGAAAAAAGGTTTTATGT+AGG | + | 42997:14375-14394 | MS.gene30663:intron | 30.0% |
| !! | GGTGGTATTTTTGTAAGTAA+AGG | + | 42997:14325-14344 | MS.gene30663:intron | 30.0% |
| !! | TTCAGTATTTTAGAGATTCC+TGG | - | 42997:14239-14258 | None:intergenic | 30.0% |
| !!! | CCTACATAAAACCTTTTTTC+AGG | - | 42997:14378-14397 | None:intergenic | 30.0% |
| !!! | CTGTTTTTAAACATCTGTGT+AGG | + | 42997:14489-14508 | MS.gene30663:intron | 30.0% |
| !!! | GCAGTTTTTTCAAATCAAAC+AGG | + | 42997:14428-14447 | MS.gene30663:intron | 30.0% |
| !!! | TAAAACCTTTTTTCAGGCAT+TGG | - | 42997:14372-14391 | None:intergenic | 30.0% |
| !!! | TACTTTTAGTTTTTCTGTGC+TGG | + | 42997:13060-13079 | MS.gene30663:intron | 30.0% |
| ACATACGTACAAGCATTTAG+TGG | - | 42997:13465-13484 | None:intergenic | 35.0% | |
| ACCCATAAAGTCAAGAATTG+AGG | + | 42997:14709-14728 | MS.gene30663:CDS | 35.0% | |
| AGGAAAGAATTAATCGAAGC+TGG | + | 42997:13721-13740 | MS.gene30663:intron | 35.0% | |
| AGTACACAATCTCAATGAAC+TGG | + | 42997:13162-13181 | MS.gene30663:CDS | 35.0% | |
| CAGATGTCACACCTTAAATT+TGG | - | 42997:13423-13442 | None:intergenic | 35.0% | |
| CATAACACGCGATGAAAATA+AGG | + | 42997:14167-14186 | MS.gene30663:intron | 35.0% | |
| CATAAGCAATGTGAAAAAGG+TGG | + | 42997:14307-14326 | MS.gene30663:intron | 35.0% | |
| GACTAAAGAATATACTAGCC+AGG | + | 42997:14218-14237 | MS.gene30663:intron | 35.0% | |
| GACTGACCTTTAAATGTTTG+AGG | - | 42997:12948-12967 | None:intergenic | 35.0% | |
| GGAAAGAATTAATCGAAGCT+GGG | + | 42997:13722-13741 | MS.gene30663:intron | 35.0% | |
| GGCTAGTATATTCTTTAGTC+AGG | - | 42997:14218-14237 | None:intergenic | 35.0% | |
| GTAAGTTCCATGAAAATGCA+AGG | - | 42997:13086-13105 | None:intergenic | 35.0% | |
| GTATATTCTTTAGTCAGGTC+AGG | - | 42997:14213-14232 | None:intergenic | 35.0% | |
| TCTACTGAAGAACCAAATTC+GGG | - | 42997:13511-13530 | None:intergenic | 35.0% | |
| TGATACAGCTACATTGAAGA+AGG | - | 42997:13113-13132 | None:intergenic | 35.0% | |
| !! | AAACAGTATTGGCATCAGTA+TGG | - | 42997:13649-13668 | None:intergenic | 35.0% |
| !! | AAAGTCTTGTTTGGAAGTGA+TGG | + | 42997:12897-12916 | MS.gene30663:CDS | 35.0% |
| !! | ACTGATGGAAAAGTCTTGTT+TGG | + | 42997:12888-12907 | MS.gene30663:CDS | 35.0% |
| !! | ATGTTTTGAATGGGATAGCT+TGG | + | 42997:14013-14032 | MS.gene30663:CDS | 35.0% |
| !! | TGTTTTGAATGGGATAGCTT+GGG | + | 42997:14014-14033 | MS.gene30663:CDS | 35.0% |
| CCAGCTTACTAGAGAATCTT+AGG | - | 42997:13309-13328 | None:intergenic | 40.0% | |
| CCATGTATGGATGATTTCTG+CGG | + | 42997:13283-13302 | MS.gene30663:intron | 40.0% | |
| CCTAAGATTCTCTAGTAAGC+TGG | + | 42997:13306-13325 | MS.gene30663:intron | 40.0% | |
| GGATTCTCCTCCCAAATTTA+AGG | + | 42997:13409-13428 | MS.gene30663:CDS | 40.0% | |
| GTCAAGAATTGAGGAAGGAA+TGG | + | 42997:14718-14737 | MS.gene30663:CDS | 40.0% | |
| TCGAAGCTGGGAATAATGTA+AGG | + | 42997:13734-13753 | MS.gene30663:intron | 40.0% | |
| TGTCACACCTTAAATTTGGG+AGG | - | 42997:13419-13438 | None:intergenic | 40.0% | |
| TTGCAGTGACTGGAAAACTA+TGG | + | 42997:14660-14679 | MS.gene30663:intron | 40.0% | |
| CAACGAAGTGATCCCGAATT+TGG | + | 42997:13496-13515 | MS.gene30663:intron | 45.0% | |
| CCAACGACAAGACCATCATT+AGG | - | 42997:13387-13406 | None:intergenic | 45.0% | |
| CCGCAGAAATCATCCATACA+TGG | - | 42997:13286-13305 | None:intergenic | 45.0% | |
| GCGAGAATCTCTCCTAATGA+TGG | + | 42997:13372-13391 | MS.gene30663:CDS | 45.0% | |
| TTCTTTAGTCAGGTCAGGTC+AGG | - | 42997:14208-14227 | None:intergenic | 45.0% | |
| TTGTTCTTGGTTGCAGTGAC+TGG | + | 42997:14650-14669 | MS.gene30663:intron | 45.0% | |
| !! | ATGATGGTCTTGTCGTTGGA+TGG | + | 42997:13388-13407 | MS.gene30663:CDS | 45.0% |
| !! | CCTAATGATGGTCTTGTCGT+TGG | + | 42997:13384-13403 | MS.gene30663:CDS | 45.0% |
| ! | GCTGAGCAGGAGCGAATTTT+TGG | + | 42997:14039-14058 | MS.gene30663:CDS | 50.0% |
| !!! | GTGCTGGCCTTGCATTTTCA+TGG | + | 42997:13076-13095 | MS.gene30663:intron | 50.0% |
| GATAGCTTGGGATGCTGAGC+AGG | + | 42997:14026-14045 | MS.gene30663:CDS | 55.0% | |
| GGTTGGGGTCTCGCAACTGA+TGG | + | 42997:12873-12892 | MS.gene30663:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 42997 | gene | 12864 | 14781 | 12864 | ID=MS.gene30663 |
| 42997 | mRNA | 12864 | 14781 | 12864 | ID=MS.gene30663.t1;Parent=MS.gene30663 |
| 42997 | exon | 12864 | 12960 | 12864 | ID=MS.gene30663.t1.exon1;Parent=MS.gene30663.t1 |
| 42997 | CDS | 12864 | 12960 | 12864 | ID=cds.MS.gene30663.t1;Parent=MS.gene30663.t1 |
| 42997 | exon | 13125 | 13222 | 13125 | ID=MS.gene30663.t1.exon2;Parent=MS.gene30663.t1 |
| 42997 | CDS | 13125 | 13222 | 13125 | ID=cds.MS.gene30663.t1;Parent=MS.gene30663.t1 |
| 42997 | exon | 13360 | 13430 | 13360 | ID=MS.gene30663.t1.exon3;Parent=MS.gene30663.t1 |
| 42997 | CDS | 13360 | 13430 | 13360 | ID=cds.MS.gene30663.t1;Parent=MS.gene30663.t1 |
| 42997 | exon | 13723 | 13750 | 13723 | ID=MS.gene30663.t1.exon4;Parent=MS.gene30663.t1 |
| 42997 | CDS | 13723 | 13750 | 13723 | ID=cds.MS.gene30663.t1;Parent=MS.gene30663.t1 |
| 42997 | exon | 14006 | 14060 | 14006 | ID=MS.gene30663.t1.exon5;Parent=MS.gene30663.t1 |
| 42997 | CDS | 14006 | 14060 | 14006 | ID=cds.MS.gene30663.t1;Parent=MS.gene30663.t1 |
| 42997 | exon | 14666 | 14781 | 14666 | ID=MS.gene30663.t1.exon6;Parent=MS.gene30663.t1 |
| 42997 | CDS | 14666 | 14781 | 14666 | ID=cds.MS.gene30663.t1;Parent=MS.gene30663.t1 |
| Gene Sequence |
| Protein sequence |
