AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34020.t1 MTR_1g095540 96.070 458 13 1 1 453 1 458 0.0 917
MS.gene34020.t1 MTR_1g095560 85.153 458 63 1 1 453 1 458 0.0 811
MS.gene34020.t1 MTR_1g095560 79.796 490 62 3 1 453 1 490 0.0 796
MS.gene34020.t1 MTR_2g062990 56.120 433 186 3 22 452 33 463 0.0 520
MS.gene34020.t1 MTR_2g063000 53.159 459 206 4 2 452 7 464 1.67e-179 510
MS.gene34020.t1 MTR_7g013790 55.217 460 186 10 8 452 11 465 4.66e-178 506
MS.gene34020.t1 MTR_2g062980 54.273 433 194 3 22 452 33 463 1.03e-176 503
MS.gene34020.t1 MTR_2g081620 53.556 450 203 6 5 450 15 462 1.67e-174 498
MS.gene34020.t1 MTR_4g075150 51.131 442 205 8 17 451 24 461 2.66e-155 448
MS.gene34020.t1 MTR_3g079560 52.900 431 190 7 24 453 74 492 3.89e-155 449
MS.gene34020.t1 MTR_4g075150 51.126 444 204 8 17 451 24 463 7.01e-155 447
MS.gene34020.t1 MTR_2g063000 48.696 460 194 6 2 452 7 433 8.94e-155 446
MS.gene34020.t1 MTR_3g079570 52.425 433 194 6 21 453 72 492 1.51e-153 445
MS.gene34020.t1 MTR_3g034290 49.070 430 209 5 23 451 70 490 1.39e-150 437
MS.gene34020.t1 MTR_3g079590 52.235 425 192 6 21 445 71 484 3.27e-150 437
MS.gene34020.t1 MTR_3g079600 52.347 426 189 6 23 445 71 485 3.21e-149 434
MS.gene34020.t1 MTR_3g079620 50.000 442 200 7 21 453 71 500 1.26e-147 430
MS.gene34020.t1 MTR_1g021010 49.309 434 203 7 14 445 62 480 2.13e-143 419
MS.gene34020.t1 MTR_3g079630 49.111 450 202 9 9 445 57 492 1.98e-142 417
MS.gene34020.t1 MTR_1g020090 48.741 437 202 7 14 445 72 491 2.16e-141 414
MS.gene34020.t1 MTR_4g122950 45.053 475 232 6 5 453 8 479 1.09e-140 412
MS.gene34020.t1 MTR_4g122960 45.243 473 232 7 5 453 8 477 2.29e-140 411
MS.gene34020.t1 MTR_4g089155 47.500 440 215 6 26 453 52 487 7.08e-140 410
MS.gene34020.t1 MTR_0003s0560 47.454 432 213 8 23 452 69 488 2.31e-138 406
MS.gene34020.t1 MTR_1g021020 48.046 435 206 7 14 445 64 481 2.36e-137 404
MS.gene34020.t1 MTR_2g012860 44.118 476 234 7 3 452 5 474 6.38e-137 402
MS.gene34020.t1 MTR_2g012850 44.350 469 237 8 3 453 5 467 3.59e-135 397
MS.gene34020.t1 MTR_3g034290 49.713 348 166 4 23 370 70 408 5.36e-121 359
MS.gene34020.t1 MTR_2g449850 41.518 448 236 10 18 453 31 464 8.47e-113 340
MS.gene34020.t1 MTR_8g099930 40.909 462 238 13 14 453 16 464 1.57e-111 337
MS.gene34020.t1 MTR_3g498755 41.270 441 236 12 26 451 13 445 1.86e-110 333
MS.gene34020.t1 MTR_3g434850 40.645 465 248 12 2 452 5 455 3.25e-110 333
MS.gene34020.t1 MTR_7g021130 41.002 439 236 12 26 451 29 457 1.40e-106 324
MS.gene34020.t1 MTR_3g090130 41.553 438 242 9 25 453 26 458 8.60e-106 322
MS.gene34020.t1 MTR_5g072570 40.590 441 246 8 25 453 27 463 1.13e-105 322
MS.gene34020.t1 MTR_3g090150 39.498 438 253 8 25 453 31 465 8.21e-101 310
MS.gene34020.t1 MTR_1g040370 38.053 452 222 13 8 451 14 415 4.86e-97 298
MS.gene34020.t1 MTR_0003s0560 49.618 262 127 4 23 284 69 325 6.10e-84 262
MS.gene34020.t1 MTR_8g011350 31.643 493 282 11 2 445 4 490 7.91e-76 245
MS.gene34020.t1 MTR_7g051430 33.811 488 266 15 8 445 10 490 1.45e-72 237
MS.gene34020.t1 MTR_7g080180 32.869 502 266 16 5 450 12 498 4.01e-71 233
MS.gene34020.t1 MTR_1g102130 33.333 447 235 13 24 451 88 490 6.68e-69 228
MS.gene34020.t1 MTR_8g011350 30.573 471 272 11 2 423 4 468 7.74e-66 219
MS.gene34020.t1 MTR_7g080180 31.739 460 244 15 5 409 12 456 1.07e-57 197
MS.gene34020.t1 MTR_2g018600 29.018 448 281 15 28 450 34 469 3.24e-54 188
MS.gene34020.t1 MTR_7g051430 33.010 412 220 14 8 370 10 414 1.09e-52 182
MS.gene34020.t1 MTR_3g100420 29.979 477 289 16 5 447 28 493 3.79e-52 183
MS.gene34020.t1 MTR_4g021520 29.613 439 281 12 27 447 39 467 5.16e-52 182
MS.gene34020.t1 MTR_2g018590 28.764 445 287 13 28 450 33 469 1.06e-51 181
MS.gene34020.t1 MTR_4g021535 29.279 444 286 12 22 447 30 463 1.79e-51 181
MS.gene34020.t1 MTR_4g021500 30.926 443 277 14 27 450 39 471 3.30e-51 186
MS.gene34020.t1 MTR_4g021500 28.935 432 267 14 40 447 487 902 9.92e-46 170
MS.gene34020.t1 MTR_4g021500 30.926 443 277 14 27 450 39 471 3.42e-51 186
MS.gene34020.t1 MTR_4g021500 28.947 418 267 13 40 447 487 884 2.92e-45 169
MS.gene34020.t1 MTR_4g021510 28.982 452 267 16 27 447 38 466 7.18e-51 179
MS.gene34020.t1 MTR_4g021490 29.385 439 282 14 27 447 39 467 1.61e-50 178
MS.gene34020.t1 MTR_4g021530 28.604 444 289 11 22 447 34 467 6.43e-50 176
MS.gene34020.t1 MTR_3g045770 28.704 432 279 11 28 442 27 446 2.49e-49 174
MS.gene34020.t1 MTR_2g018630 27.500 440 291 13 28 450 37 465 1.68e-46 167
MS.gene34020.t1 MTR_4g021520 29.500 400 255 11 27 409 39 428 6.60e-43 157
MS.gene34020.t1 MTR_0075s0010 31.948 385 179 16 25 397 5 318 2.64e-41 150
MS.gene34020.t1 MTR_2g095730 26.905 420 276 12 42 448 94 495 9.64e-40 149
MS.gene34020.t1 MTR_1g050545 28.777 417 261 15 40 442 81 475 2.93e-39 148
MS.gene34020.t1 MTR_5g097180 28.333 420 263 13 44 450 43 437 1.24e-38 145
MS.gene34020.t1 MTR_4g057585 26.606 436 265 12 33 445 87 490 5.55e-38 144
MS.gene34020.t1 MTR_2g095750 25.822 426 270 12 42 448 100 498 1.69e-37 143
MS.gene34020.t1 MTR_4g084710 28.369 423 259 15 44 450 45 439 1.93e-37 142
MS.gene34020.t1 MTR_4g057570 24.831 443 286 12 27 448 74 490 1.02e-36 141
MS.gene34020.t1 MTR_4g057595 26.168 428 261 12 42 446 97 492 1.19e-36 141
MS.gene34020.t1 MTR_1g094155 27.561 410 266 14 44 442 92 481 9.67e-36 138
MS.gene34020.t1 MTR_4g057600 26.620 432 258 16 42 448 95 492 3.10e-35 137
MS.gene34020.t1 MTR_2g009200 26.681 461 273 15 12 445 12 434 1.40e-34 134
MS.gene34020.t1 MTR_4g021535 26.577 444 251 15 22 447 30 416 2.52e-34 133
MS.gene34020.t1 MTR_2g018630 25.706 354 243 10 107 448 13 358 1.45e-30 121
MS.gene34020.t1 MTR_2g095710 25.481 416 246 13 57 448 106 481 7.93e-28 115
MS.gene34020.t1 MTR_1g051175 41.085 129 64 5 22 145 2 123 7.54e-19 86.7
MS.gene34020.t1 MTR_4g056520 22.426 437 288 16 45 447 36 455 3.61e-15 77.8
MS.gene34020.t1 MTR_4g122970 47.458 59 29 1 19 77 31 87 6.16e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34020.t1 AT3G07990 74.023 435 113 0 17 451 23 457 0.0 685
MS.gene34020.t1 AT2G35780 68.650 437 131 3 17 451 19 451 0.0 638
MS.gene34020.t1 AT4G30610 56.438 466 184 8 2 453 4 464 0.0 531
MS.gene34020.t1 AT2G24000 56.823 447 180 6 17 453 20 463 0.0 520
MS.gene34020.t1 AT2G35770 55.286 454 192 6 5 453 13 460 2.20e-176 502
MS.gene34020.t1 AT2G24010 55.402 435 189 4 20 453 23 453 1.36e-175 499
MS.gene34020.t1 AT3G02110 54.911 448 182 7 21 453 31 473 2.01e-174 497
MS.gene34020.t1 AT4G30810 54.292 431 192 2 18 445 28 456 7.27e-173 494
MS.gene34020.t1 AT2G24010 53.333 450 190 5 20 453 23 468 1.57e-170 487
MS.gene34020.t1 AT2G24010 50.749 467 185 6 28 453 2 464 3.83e-162 466
MS.gene34020.t1 AT5G23210 50.218 458 198 8 20 452 44 496 5.29e-153 444
MS.gene34020.t1 AT3G63470 48.284 437 206 8 23 453 77 499 5.11e-148 431
MS.gene34020.t1 AT3G17180 48.000 450 215 8 20 453 31 477 9.43e-140 409
MS.gene34020.t1 AT1G61130 46.638 461 233 8 1 453 7 462 1.52e-139 408
MS.gene34020.t1 AT5G08260 42.977 477 236 10 5 452 8 477 2.16e-135 398
MS.gene34020.t1 AT1G11080 45.513 468 232 6 5 453 28 491 1.31e-133 394
MS.gene34020.t1 AT1G11080 45.513 468 232 6 5 453 28 491 1.51e-133 394
MS.gene34020.t1 AT5G23210 49.395 413 179 8 20 407 44 451 5.52e-133 391
MS.gene34020.t1 AT3G52020 47.018 436 216 11 21 451 73 498 1.90e-131 389
MS.gene34020.t1 AT4G15100 44.515 474 235 9 4 453 14 483 7.84e-130 384
MS.gene34020.t1 AT2G24010 53.203 359 148 5 28 370 2 356 3.57e-129 378
MS.gene34020.t1 AT5G23210 48.635 403 180 6 75 452 1 401 1.06e-127 376
MS.gene34020.t1 AT5G23210 48.861 395 175 6 83 452 8 400 2.92e-126 372
MS.gene34020.t1 AT2G05850 42.889 450 240 9 11 453 47 486 1.08e-121 363
MS.gene34020.t1 AT3G17180 45.721 409 204 7 20 413 31 436 1.34e-120 359
MS.gene34020.t1 AT3G52000 43.694 444 234 12 14 453 50 481 1.58e-117 353
MS.gene34020.t1 AT3G52010 41.928 446 239 11 16 453 53 486 1.16e-116 350
MS.gene34020.t1 AT2G33530 43.805 452 225 13 17 451 23 462 2.75e-113 341
MS.gene34020.t1 AT1G28110 41.497 441 231 11 26 451 30 458 3.16e-107 325
MS.gene34020.t1 AT1G28110 41.497 441 231 11 26 451 30 458 3.16e-107 325
MS.gene34020.t1 AT5G23210 47.714 350 156 6 83 407 8 355 1.72e-106 320
MS.gene34020.t1 AT2G12480 39.540 435 253 6 24 452 28 458 9.97e-106 322
MS.gene34020.t1 AT1G43780 40.498 442 242 9 25 451 35 470 3.50e-105 321
MS.gene34020.t1 AT5G42240 40.724 442 246 8 24 453 29 466 4.37e-105 320
MS.gene34020.t1 AT2G24000 41.834 447 184 9 17 453 20 400 8.12e-105 317
MS.gene34020.t1 AT2G12480 38.479 434 235 7 24 452 28 434 2.41e-99 305
MS.gene34020.t1 AT2G12480 38.249 434 234 7 24 452 28 432 3.08e-98 302
MS.gene34020.t1 AT2G12480 38.161 435 234 8 24 452 28 433 9.07e-97 298
MS.gene34020.t1 AT5G42230 37.642 441 259 8 24 452 25 461 2.44e-96 298
MS.gene34020.t1 AT5G42230 37.642 441 259 8 24 452 25 461 2.44e-96 298
MS.gene34020.t1 AT4G12910 33.266 493 275 11 5 450 12 497 1.40e-81 261
MS.gene34020.t1 AT3G25420 32.136 501 282 13 1 450 1 494 4.01e-76 246
MS.gene34020.t1 AT3G56540 65.497 171 55 3 14 184 66 232 1.27e-73 232
MS.gene34020.t1 AT4G12910 32.151 451 253 10 5 409 12 455 7.80e-68 223
MS.gene34020.t1 AT2G22990 31.090 431 265 13 28 448 23 431 1.40e-60 204
MS.gene34020.t1 AT2G22920 30.254 433 270 13 28 450 25 435 1.72e-58 198
MS.gene34020.t1 AT2G22970 30.947 433 265 14 28 450 25 433 1.75e-58 198
MS.gene34020.t1 AT2G22990 30.882 408 251 12 28 425 23 409 1.16e-54 189
MS.gene34020.t1 AT2G22990 30.958 407 250 12 28 424 23 408 1.23e-54 187
MS.gene34020.t1 AT1G73270 31.034 435 262 13 28 450 33 441 3.48e-54 187
MS.gene34020.t1 AT1G73270 31.034 435 262 13 28 450 33 441 4.88e-54 187
MS.gene34020.t1 AT2G23000 28.538 431 278 13 28 448 25 435 6.76e-54 186
MS.gene34020.t1 AT2G23000 28.538 431 278 13 28 448 25 435 6.76e-54 186
MS.gene34020.t1 AT1G73280 30.296 439 260 14 28 450 33 441 8.91e-54 186
MS.gene34020.t1 AT1G73300 30.394 431 270 12 28 450 33 441 1.35e-53 186
MS.gene34020.t1 AT1G73300 30.394 431 270 12 28 450 33 441 1.35e-53 186
MS.gene34020.t1 AT2G22990 29.792 433 263 14 28 450 23 424 4.61e-53 184
MS.gene34020.t1 AT1G73290 29.358 436 266 12 28 450 32 438 2.77e-52 182
MS.gene34020.t1 AT2G22970 30.144 418 259 13 28 435 25 419 3.65e-52 182
MS.gene34020.t1 AT2G23010 28.953 449 289 13 10 448 7 435 7.14e-52 181
MS.gene34020.t1 AT2G22970 30.392 408 251 13 28 425 25 409 1.61e-51 179
MS.gene34020.t1 AT2G23010 28.731 449 290 13 10 448 7 435 2.60e-51 179
MS.gene34020.t1 AT2G22920 30.102 392 244 11 28 410 25 395 1.46e-49 174
MS.gene34020.t1 AT3G10450 28.538 431 278 12 28 450 29 437 1.85e-49 174
MS.gene34020.t1 AT5G36180 29.837 429 271 12 28 448 33 439 2.31e-49 174
MS.gene34020.t1 AT2G22980 29.206 428 265 12 28 445 26 425 3.33e-49 174
MS.gene34020.t1 AT3G12203 28.704 432 275 11 28 450 9 416 9.03e-49 172
MS.gene34020.t1 AT3G12203 28.704 432 275 11 28 450 30 437 1.09e-48 172
MS.gene34020.t1 AT3G12240 28.571 434 274 14 28 450 28 436 2.74e-47 169
MS.gene34020.t1 AT3G12230 28.802 434 273 13 28 450 27 435 4.32e-47 168
MS.gene34020.t1 AT1G73310 28.604 437 266 14 28 448 33 439 4.36e-47 168
MS.gene34020.t1 AT1G33540 28.090 445 289 13 28 450 29 464 1.01e-46 168
MS.gene34020.t1 AT3G12220 29.032 434 268 12 28 448 27 433 2.05e-46 166
MS.gene34020.t1 AT3G10450 28.224 411 265 12 46 448 4 392 3.80e-46 164
MS.gene34020.t1 AT5G09640 28.348 448 283 14 28 448 27 463 3.91e-46 166
MS.gene34020.t1 AT1G73290 29.114 395 240 10 28 410 32 398 1.31e-44 160
MS.gene34020.t1 AT5G22980 28.638 426 260 14 40 448 96 494 1.57e-44 162
MS.gene34020.t1 AT1G33540 27.869 427 277 13 46 450 4 421 5.15e-44 159
MS.gene34020.t1 AT2G22980 28.230 418 263 11 28 435 26 416 5.94e-44 159
MS.gene34020.t1 AT2G22980 28.676 408 254 11 28 425 26 406 1.67e-43 158
MS.gene34020.t1 AT3G12203 28.429 401 254 11 57 448 1 377 3.13e-43 156
MS.gene34020.t1 AT3G10450 28.079 406 263 11 28 425 29 413 1.13e-42 155
MS.gene34020.t1 AT2G22990 30.655 336 209 10 118 448 1 317 2.31e-42 152
MS.gene34020.t1 AT3G12220 28.365 416 258 12 46 448 15 403 3.95e-42 154
MS.gene34020.t1 AT2G22990 30.357 336 210 10 118 448 1 317 7.44e-42 151
MS.gene34020.t1 AT1G73310 28.537 410 249 12 28 422 33 413 9.77e-42 154
MS.gene34020.t1 AT3G10410 27.230 426 278 11 33 447 90 494 5.10e-41 153
MS.gene34020.t1 AT3G45010 26.932 427 282 11 33 448 92 499 2.33e-40 151
MS.gene34020.t1 AT3G10450 27.577 388 252 11 46 425 4 370 3.13e-39 145
MS.gene34020.t1 AT2G22920 31.068 309 184 10 28 327 25 313 1.19e-38 142
MS.gene34020.t1 AT1G73280 33.086 269 162 7 28 282 33 297 7.30e-38 142
MS.gene34020.t1 AT2G22970 31.343 268 168 7 28 286 25 285 1.08e-37 140
MS.gene34020.t1 AT1G73290 32.917 240 152 4 28 262 32 267 2.86e-37 139
MS.gene34020.t1 AT1G73280 28.045 353 216 11 107 448 12 337 1.29e-36 137
MS.gene34020.t1 AT1G73280 28.024 339 206 11 121 448 1 312 4.76e-34 130
MS.gene34020.t1 AT5G36180 27.826 345 227 9 107 448 12 337 1.77e-32 126
MS.gene34020.t1 AT5G36180 27.826 345 227 9 107 448 12 337 1.77e-32 126
MS.gene34020.t1 AT1G15000 28.141 398 253 11 44 433 37 409 1.14e-30 123
MS.gene34020.t1 AT2G22980 29.102 323 192 10 28 339 26 322 2.08e-30 121
MS.gene34020.t1 AT3G12220 32.877 219 136 5 28 240 27 240 5.70e-28 114
MS.gene34020.t1 AT3G12220 29.252 147 99 4 303 448 239 381 6.43e-11 64.3
MS.gene34020.t1 AT5G36180 27.019 322 214 8 107 425 12 315 5.49e-27 110
MS.gene34020.t1 AT5G36180 26.176 340 219 8 107 432 12 333 1.30e-25 107
MS.gene34020.t1 AT1G73310 26.380 326 204 9 107 422 12 311 2.46e-25 106
MS.gene34020.t1 AT2G22980 26.518 313 201 8 118 425 1 289 3.43e-25 105
MS.gene34020.t1 AT2G22920 26.838 272 176 9 184 450 3 256 9.03e-24 100
MS.gene34020.t1 AT5G22960 31.034 174 96 4 33 206 25 174 1.85e-21 92.0
MS.gene34020.t1 AT2G27920 23.474 426 273 19 45 434 37 445 3.11e-16 81.3
MS.gene34020.t1 AT2G22960 30.597 134 88 4 316 448 53 182 1.14e-14 72.8

Find 101 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAAACATTGGCTGTGATTT+TGG 0.097316 1.4:-74219625 MS.gene34020:CDS
TTTGTTTCTGGGAATTTCTT+TGG 0.202436 1.4:-74221363 MS.gene34020:CDS
GATGATTATCATGATTATAT+TGG 0.232593 1.4:-74219703 MS.gene34020:CDS
GTGGTACTGTTTGGGATTAT+TGG 0.264427 1.4:-74219118 MS.gene34020:CDS
AATTGTTAACCAGAGAAATA+AGG 0.271531 1.4:-74219876 MS.gene34020:CDS
GTTGGATTTGAACAGTATTC+AGG 0.275404 1.4:-74221278 MS.gene34020:CDS
GCACTTCATGCCAATGTAAC+TGG 0.277277 1.4:-74219271 MS.gene34020:CDS
GGTTGTTCTTCCATAGCTTA+TGG 0.284743 1.4:-74221140 MS.gene34020:CDS
TAAAACTAGTGGCTTTGAAT+TGG 0.298079 1.4:+74221181 None:intergenic
TTCTTGACAGTGGTACTGTT+TGG 0.300868 1.4:-74219127 MS.gene34020:intron
TGAAGTGCCTTCTGAACTTC+TGG 0.312729 1.4:+74219286 None:intergenic
AATCCCTTAAAATTTATAAC+TGG 0.318228 1.4:+74219842 None:intergenic
TCTTGACAGTGGTACTGTTT+GGG 0.320433 1.4:-74219126 MS.gene34020:intron
TATGGTGCTTCTGAAGAAAT+TGG 0.320537 1.4:-74221122 MS.gene34020:CDS
TTAATAAGTTCTTGATATAT+AGG 0.324336 1.4:+74219071 None:intergenic
AACGCATCTGATTAACTTGA+TGG 0.354191 1.4:+74218159 None:intergenic
GGATGGAGTCAAGTTTATAA+AGG 0.364164 1.4:-74218285 MS.gene34020:CDS
TACAGATTCCCTTATTTCTC+TGG 0.376213 1.4:+74219867 None:intergenic
TCCATCAGTGCAATGTTTGC+AGG 0.379315 1.4:-74219590 MS.gene34020:CDS
TTACAATTGTTCCAAGCATA+AGG 0.383194 1.4:+74221056 None:intergenic
TGGTACCCTTGGTATGATAA+TGG 0.388411 1.4:-74218558 MS.gene34020:CDS
AAAACTAGTGGCTTTGAATT+GGG 0.395531 1.4:+74221182 None:intergenic
AATGTTTAGCTTCTTATATG+TGG 0.413578 1.4:+74219639 None:intergenic
ACTGCAACTCGATATTCTAT+TGG 0.429971 1.4:-74218609 MS.gene34020:CDS
CTCGAGTCTCCTGCTGGTGT+TGG 0.436875 1.4:-74220921 MS.gene34020:CDS
CATAGAGAATTCTACATGGC+TGG 0.440477 1.4:-74220038 MS.gene34020:CDS
ATAAGCTATGGAAGAACAAC+CGG 0.440804 1.4:+74221142 None:intergenic
ATTGTTAACCAGAGAAATAA+GGG 0.444696 1.4:-74219875 MS.gene34020:CDS
TTTACTTGAATCCTTATGCT+TGG 0.446628 1.4:-74221067 MS.gene34020:CDS
ATATTGGAACATTTGAGTAC+TGG 0.448006 1.4:-74219687 MS.gene34020:CDS
GATGATGGTTGGTAGCTTCA+AGG 0.449314 1.4:+74218584 None:intergenic
AACTTATTAATGCCGGTCTC+CGG 0.457058 1.4:-74219058 MS.gene34020:CDS
GATGGAGTCAAGTTTATAAA+GGG 0.457624 1.4:-74218284 MS.gene34020:CDS
ACTGAGCAAGGAAACATTGA+TGG 0.464521 1.4:-74219550 MS.gene34020:CDS
CCATCATCAATTGGTACCCT+TGG 0.465365 1.4:-74218569 MS.gene34020:CDS
GATAAAATTAAGTGGTTACC+AGG 0.466526 1.4:-74221314 MS.gene34020:CDS
ACATTTGGTGACCAGAAAAC+AGG 0.467712 1.4:-74220864 MS.gene34020:intron
TAACTGGGATTTCATATGCT+TGG 0.468731 1.4:-74219255 MS.gene34020:CDS
TTGGTCACAGTAAGAGGTGC+TGG 0.469247 1.4:-74218255 MS.gene34020:CDS
ATATAGGAAGCATAGACAAT+GGG 0.471053 1.4:+74219087 None:intergenic
GTCTCCGGACATGGGTATAC+AGG 0.472161 1.4:-74219043 MS.gene34020:intron
CTCACATTGGTCACAGTAAG+AGG 0.474837 1.4:-74218261 MS.gene34020:CDS
ACAACAGATATGCAGACATT+TGG 0.477904 1.4:-74220879 MS.gene34020:CDS
CATATGCTTGGAAGGCATGC+AGG 0.479209 1.4:-74219243 MS.gene34020:intron
AAGTAAAGTGACTTGCCATC+AGG 0.482213 1.4:+74221083 None:intergenic
ATAGAGAATTCTACATGGCT+GGG 0.484144 1.4:-74220037 MS.gene34020:CDS
AGCTACCAACCATCATCAAT+TGG 0.484161 1.4:-74218578 MS.gene34020:CDS
TTCAGAAGCACCATAAGCTA+TGG 0.487990 1.4:+74221130 None:intergenic
GCCTGCAAACATTGCACTGA+TGG 0.490912 1.4:+74219589 None:intergenic
TTATGGCTTAATGGTGGTCC+CGG 0.492550 1.4:-74221161 MS.gene34020:CDS
AGGTCATTATGTTCTTCAGT+TGG 0.495687 1.4:-74219903 MS.gene34020:CDS
CTGTTCCTCGAGTCTCCTGC+TGG 0.503070 1.4:-74220927 MS.gene34020:CDS
TAAGAAAAGCCAACACCAGC+AGG 0.507655 1.4:+74220912 None:intergenic
TTAATGCCGGTCTCCGGACA+TGG 0.511891 1.4:-74219052 MS.gene34020:CDS
CACTTCATGCCAATGTAACT+GGG 0.519280 1.4:-74219270 MS.gene34020:CDS
TGGGATTTCATATGCTTGGA+AGG 0.530564 1.4:-74219251 MS.gene34020:CDS
AAAAGAGAGATAAAATTAAG+TGG 0.531113 1.4:-74221322 MS.gene34020:CDS
TATCAAGAACTTATTAATGC+CGG 0.540455 1.4:-74219065 MS.gene34020:CDS
CCATCAGGCCTAATACGAAA+AGG 0.543223 1.4:+74221098 None:intergenic
ACAGTTGCAATTACTGAGCA+AGG 0.545839 1.4:-74219562 MS.gene34020:CDS
ATGGCTGGGGAAAGTTATGC+AGG 0.552095 1.4:-74220023 MS.gene34020:intron
GGTACCTGTATACCCATGTC+CGG 0.557023 1.4:+74219039 None:intergenic
TTGCACTGATGGATGCTGAA+TGG 0.559164 1.4:+74219600 None:intergenic
GAATATCGAGTTGCAGTCAG+TGG 0.560041 1.4:+74218615 None:intergenic
CCAAGGGTACCAATTGATGA+TGG 0.575493 1.4:+74218569 None:intergenic
ATATGAAATCCCAGTTACAT+TGG 0.575710 1.4:+74219261 None:intergenic
GTATACCCATGTCCGGAGAC+CGG 0.578010 1.4:+74219046 None:intergenic
TTGGAACATTTGAGTACTGG+TGG 0.579804 1.4:-74219684 MS.gene34020:CDS
AGTAAGAGGTGCTGGGCATG+AGG 0.583918 1.4:-74218247 MS.gene34020:CDS
ACTGAAGCACCACTGAGTCA+TGG 0.585903 1.4:-74221206 MS.gene34020:CDS
TCAGAATACCTTTCAGCACA+TGG 0.589369 1.4:+74219325 None:intergenic
CATATGATCCATGTGCTGAA+AGG 0.595074 1.4:-74219333 MS.gene34020:CDS
TTTGAGTACTGGTGGACACA+TGG 0.597648 1.4:-74219676 MS.gene34020:CDS
TCATATGCTCTATACATCCA+AGG 0.600430 1.4:+74219349 None:intergenic
GGGTACCAATTGATGATGGT+TGG 0.601605 1.4:+74218573 None:intergenic
TAGAGAATTCTACATGGCTG+GGG 0.605545 1.4:-74220036 MS.gene34020:CDS
AGGCTATGTGACTGTGAATG+AGG 0.607173 1.4:-74221258 MS.gene34020:CDS
TATATAGGAAGCATAGACAA+TGG 0.610027 1.4:+74219086 None:intergenic
TAATGCCGGTCTCCGGACAT+GGG 0.613778 1.4:-74219051 MS.gene34020:CDS
TGGTCACAGTAAGAGGTGCT+GGG 0.613893 1.4:-74218254 MS.gene34020:CDS
CAATCGTCCAGAAGTTCAGA+AGG 0.617406 1.4:-74219293 MS.gene34020:CDS
GTGACTGTGAATGAGGAGAG+TGG 0.618598 1.4:-74221251 MS.gene34020:CDS
ACATATAAGAAGCTAAACAT+TGG 0.621226 1.4:-74219637 MS.gene34020:CDS
CAACACCAGCAGGAGACTCG+AGG 0.627276 1.4:+74220922 None:intergenic
ACCTTGCCATTATCATACCA+AGG 0.630379 1.4:+74218552 None:intergenic
GCTTCACGAGGCTTATGAAG+AGG 0.634672 1.4:+74218222 None:intergenic
AATTCTCTATGTTTGTACTG+AGG 0.640776 1.4:+74220050 None:intergenic
AGTGATGTGGTATTGTTACA+AGG 0.643653 1.4:+74219508 None:intergenic
CAAACATAGAGAATTCTACA+TGG 0.646522 1.4:-74220042 MS.gene34020:CDS
AGTTTATAAAGGGCTCACAT+TGG 0.647641 1.4:-74218274 MS.gene34020:CDS
CCCTTGGTATGATAATGGCA+AGG 0.647659 1.4:-74218553 MS.gene34020:intron
ATAAAATTAAGTGGTTACCA+GGG 0.651346 1.4:-74221313 MS.gene34020:CDS
AATACCACATCACTCAGAAG+CGG 0.652328 1.4:-74219499 MS.gene34020:CDS
TAAGCTATGGAAGAACAACC+GGG 0.663016 1.4:+74221143 None:intergenic
CCATTAAGCCATAAAACTAG+TGG 0.663685 1.4:+74221170 None:intergenic
CCTTGCCATTATCATACCAA+GGG 0.676224 1.4:+74218553 None:intergenic
GAATTGGGTCCATGACTCAG+TGG 0.681910 1.4:+74221197 None:intergenic
TATAGGAAGCATAGACAATG+GGG 0.694959 1.4:+74219088 None:intergenic
AAAAGAATAAATGCTTCACG+AGG 0.727158 1.4:+74218210 None:intergenic
CAAGCCGCTTCTGAGTGATG+TGG 0.728663 1.4:+74219495 None:intergenic
GATGTGGTATTGTTACAAGG+TGG 0.753464 1.4:+74219511 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAACTTAATATTGTAT+TGG - chr1.4:74221110-74221129 MS.gene34020:CDS 10.0%
!! GTAGTTAAATATATATTATT+AGG - chr1.4:74219165-74219184 MS.gene34020:intron 10.0%
!!! ACAATATTAAGTTTTTATAT+TGG + chr1.4:74221108-74221127 None:intergenic 10.0%
!!! GTTACTAATATTTATTATTT+TGG - chr1.4:74218589-74218608 MS.gene34020:CDS 10.0%
!! CATGTGATTAATAAATTTAT+TGG + chr1.4:74219092-74219111 None:intergenic 15.0%
!! TTAATAAGTTCTTGATATAT+AGG + chr1.4:74220491-74220510 None:intergenic 15.0%
!!! AAAACTTATGAATTTGTTTT+GGG - chr1.4:74219200-74219219 MS.gene34020:intron 15.0%
!!! AATCCAGTTATAAATTTTAA+GGG - chr1.4:74219714-74219733 MS.gene34020:CDS 15.0%
!!! ATTAATAAATTTATTGGTCT+TGG + chr1.4:74219086-74219105 None:intergenic 15.0%
!!! ATTAATCACATGATAATTTT+TGG - chr1.4:74219100-74219119 MS.gene34020:CDS 15.0%
!!! CATATATTTTTCTAGTTAAT+TGG - chr1.4:74219474-74219493 MS.gene34020:intron 15.0%
!!! TAAAACTTATGAATTTGTTT+TGG - chr1.4:74219199-74219218 MS.gene34020:intron 15.0%
!!! TAATCCAGTTATAAATTTTA+AGG - chr1.4:74219713-74219732 MS.gene34020:CDS 15.0%
!!! TATAAATTTTAAGGGATTTA+TGG - chr1.4:74219722-74219741 MS.gene34020:CDS 15.0%
!! AAAAGAGAGATAAAATTAAG+TGG - chr1.4:74218237-74218256 MS.gene34020:CDS 20.0%
!! AATCCCTTAAAATTTATAAC+TGG + chr1.4:74219720-74219739 None:intergenic 20.0%
!! ATTAAATTTAACCGATAAGT+GGG - chr1.4:74218833-74218852 MS.gene34020:intron 20.0%
!! GATGATTATCATGATTATAT+TGG - chr1.4:74219856-74219875 MS.gene34020:CDS 20.0%
!! TATTAAATTTAACCGATAAG+TGG - chr1.4:74218832-74218851 MS.gene34020:intron 20.0%
!! TATTCATTCTTCTTTATATG+TGG + chr1.4:74218791-74218810 None:intergenic 20.0%
!!! ATTCTATCATGATTTTGAAT+TGG - chr1.4:74220394-74220413 MS.gene34020:intron 20.0%
!!! GTTATTGTCAGTTTTAATTT+TGG + chr1.4:74218909-74218928 None:intergenic 20.0%
!!! TATTCTTTTTAGATCATTCT+TGG - chr1.4:74221363-74221382 MS.gene34020:CDS 20.0%
!!! TTGTTTATATTTAAAACCAG+AGG + chr1.4:74220567-74220586 None:intergenic 20.0%
!!! TTTCTGTTTGTACTTATTTT+TGG - chr1.4:74219126-74219145 MS.gene34020:intron 20.0%
! AAGTATATAGTTAAGGTTGA+TGG + chr1.4:74220544-74220563 None:intergenic 25.0%
! AATCTCATCCATTTCATATT+CGG - chr1.4:74220853-74220872 MS.gene34020:intron 25.0%
! AATTGTTAACCAGAGAAATA+AGG - chr1.4:74219683-74219702 MS.gene34020:CDS 25.0%
! ACATATAAGAAGCTAAACAT+TGG - chr1.4:74219922-74219941 MS.gene34020:intron 25.0%
! ACATGTATCTGGTATAAATT+TGG + chr1.4:74220611-74220630 None:intergenic 25.0%
! ACTTCTACTAATACATGTTA+TGG + chr1.4:74220145-74220164 None:intergenic 25.0%
! ATAAAATTAAGTGGTTACCA+GGG - chr1.4:74218246-74218265 MS.gene34020:CDS 25.0%
! ATGAGATAATGAGATTATCA+AGG + chr1.4:74221229-74221248 None:intergenic 25.0%
! ATTGTTAACCAGAGAAATAA+GGG - chr1.4:74219684-74219703 MS.gene34020:CDS 25.0%
! TAAGTGAAATTTCGTTTCAT+AGG + chr1.4:74220680-74220699 None:intergenic 25.0%
! TAGATTTAAATGATTTCCTC+AGG - chr1.4:74219636-74219655 MS.gene34020:CDS 25.0%
! TATCAAGAACTTATTAATGC+CGG - chr1.4:74220494-74220513 MS.gene34020:intron 25.0%
! TTAAATTTAACCGATAAGTG+GGG - chr1.4:74218834-74218853 MS.gene34020:intron 25.0%
! TTGCTAAAACAAAACAAATC+TGG + chr1.4:74219389-74219408 None:intergenic 25.0%
!! AATGTTTAGCTTCTTATATG+TGG + chr1.4:74219923-74219942 None:intergenic 25.0%
!! AGCTAACATAAGTTTTACAA+GGG - chr1.4:74218755-74218774 MS.gene34020:intron 25.0%
!! ATCAGTTTTCTACTTATCAA+TGG - chr1.4:74219577-74219596 MS.gene34020:CDS 25.0%
!! CATAATTTTCTCTTTTGTGA+AGG - chr1.4:74221246-74221265 MS.gene34020:CDS 25.0%
!! TATCATGATTTTGAATTGGA+AGG - chr1.4:74220398-74220417 MS.gene34020:intron 25.0%
!! TTTTCTACTTATCAATGGAA+TGG - chr1.4:74219582-74219601 MS.gene34020:CDS 25.0%
!!! AAAGACATTGTTAGTTTGTT+TGG + chr1.4:74218996-74219015 None:intergenic 25.0%
!!! AACTAACAATGTCTTTTTCA+AGG - chr1.4:74219000-74219019 MS.gene34020:intron 25.0%
!!! ATTTTGAAAGGATTGGTTAT+AGG - chr1.4:74221197-74221216 MS.gene34020:CDS 25.0%
!!! ATTTTTGGCTTATAGTTGAT+TGG - chr1.4:74219141-74219160 MS.gene34020:intron 25.0%
!!! TGTTCAAATCCAACATTTTT+TGG + chr1.4:74218275-74218294 None:intergenic 25.0%
AAAACTGACAATAACTGTCT+AGG - chr1.4:74218914-74218933 MS.gene34020:intron 30.0%
AAAAGAATAAATGCTTCACG+AGG + chr1.4:74221352-74221371 None:intergenic 30.0%
AATTCTCTATGTTTGTACTG+AGG + chr1.4:74219512-74219531 None:intergenic 30.0%
ATAATGAGATTATCAAGGCA+GGG + chr1.4:74221224-74221243 None:intergenic 30.0%
ATATGAAATCCCAGTTACAT+TGG + chr1.4:74220301-74220320 None:intergenic 30.0%
ATATTGGAACATTTGAGTAC+TGG - chr1.4:74219872-74219891 MS.gene34020:CDS 30.0%
CAAACATAGAGAATTCTACA+TGG - chr1.4:74219517-74219536 MS.gene34020:CDS 30.0%
CTAGTTTACCGAATATGAAA+TGG + chr1.4:74220864-74220883 None:intergenic 30.0%
GATAAAATTAAGTGGTTACC+AGG - chr1.4:74218245-74218264 MS.gene34020:CDS 30.0%
GATGGAGTCAAGTTTATAAA+GGG - chr1.4:74221275-74221294 MS.gene34020:CDS 30.0%
GTTTGGTACAAAGTCAATTA+CGG + chr1.4:74218979-74218998 None:intergenic 30.0%
TAAATTTAACCGATAAGTGG+GGG - chr1.4:74218835-74218854 MS.gene34020:intron 30.0%
TGATGCATAGGTTTGTAAAA+TGG - chr1.4:74219334-74219353 MS.gene34020:CDS 30.0%
TTACAATTGTTCCAAGCATA+AGG + chr1.4:74218506-74218525 None:intergenic 30.0%
TTTACTTGAATCCTTATGCT+TGG - chr1.4:74218492-74218511 MS.gene34020:intron 30.0%
! AAATTGGTCCTTTTCGTATT+AGG - chr1.4:74218453-74218472 MS.gene34020:intron 30.0%
! ACAATGCTTCTAAAAGTGAT+GGG + chr1.4:74220787-74220806 None:intergenic 30.0%
! ATATAGGAAGCATAGACAAT+GGG + chr1.4:74220475-74220494 None:intergenic 30.0%
! CTAACATAAGTTTTACAAGG+GGG - chr1.4:74218757-74218776 MS.gene34020:intron 30.0%
! GAGCTAACATAAGTTTTACA+AGG - chr1.4:74218754-74218773 MS.gene34020:intron 30.0%
! GCTAACATAAGTTTTACAAG+GGG - chr1.4:74218756-74218775 MS.gene34020:intron 30.0%
! TATATAGGAAGCATAGACAA+TGG + chr1.4:74220476-74220495 None:intergenic 30.0%
! TCATAATCGCAGATTTTGAA+AGG - chr1.4:74221185-74221204 MS.gene34020:CDS 30.0%
! TCTTCTGAACTTTCGTATTT+TGG - chr1.4:74220893-74220912 MS.gene34020:CDS 30.0%
! TGAATGAGTTGAAAGCATAT+AGG + chr1.4:74220107-74220126 None:intergenic 30.0%
! TGCTTGTATTTAAGACTAGA+GGG - chr1.4:74220637-74220656 MS.gene34020:intron 30.0%
! TTGCTTGTATTTAAGACTAG+AGG - chr1.4:74220636-74220655 MS.gene34020:intron 30.0%
!! AAAACTAGTGGCTTTGAATT+GGG + chr1.4:74218380-74218399 None:intergenic 30.0%
!! TAAAACTAGTGGCTTTGAAT+TGG + chr1.4:74218381-74218400 None:intergenic 30.0%
!! TAAACATTGGCTGTGATTTT+GGG - chr1.4:74219935-74219954 MS.gene34020:intron 30.0%
!! TCTAGTCTTAAATACAAGCA+AGG + chr1.4:74220637-74220656 None:intergenic 30.0%
!! TTTGTTTCTGGGAATTTCTT+TGG - chr1.4:74218196-74218215 MS.gene34020:CDS 30.0%
!!! ATTCAAAGCCACTAGTTTTA+TGG - chr1.4:74218381-74218400 MS.gene34020:intron 30.0%
!!! ATTCTGTGTTGTTTTGTTTC+TGG - chr1.4:74218184-74218203 MS.gene34020:CDS 30.0%
!!! CGTTTTTTCTATCATGTCAA+AGG - chr1.4:74221051-74221070 MS.gene34020:intron 30.0%
!!! TATTATTTTGGATGTTGCAG+TGG - chr1.4:74218601-74218620 MS.gene34020:CDS 30.0%
!!! TTCTGTGTTGTTTTGTTTCT+GGG - chr1.4:74218185-74218204 MS.gene34020:CDS 30.0%
!!! TTTTTTCTTCTTCTTGACAG+TGG - chr1.4:74220422-74220441 MS.gene34020:intron 30.0%
ACAACAGATATGCAGACATT+TGG - chr1.4:74218680-74218699 MS.gene34020:intron 35.0%
ACTGCAACTCGATATTCTAT+TGG - chr1.4:74220950-74220969 MS.gene34020:intron 35.0%
AGGTCATTATGTTCTTCAGT+TGG - chr1.4:74219656-74219675 MS.gene34020:CDS 35.0%
AGTGATGTGGTATTGTTACA+AGG + chr1.4:74220054-74220073 None:intergenic 35.0%
AGTTTATAAAGGGCTCACAT+TGG - chr1.4:74221285-74221304 MS.gene34020:CDS 35.0%
ATAAGCTATGGAAGAACAAC+CGG + chr1.4:74218420-74218439 None:intergenic 35.0%
ATAGAGAATTCTACATGGCT+GGG - chr1.4:74219522-74219541 MS.gene34020:CDS 35.0%
CCATTAAGCCATAAAACTAG+TGG + chr1.4:74218392-74218411 None:intergenic 35.0%
CCTTAACTATATACTTCCTC+TGG - chr1.4:74220548-74220567 MS.gene34020:intron 35.0%
GATAATGAGATTATCAAGGC+AGG + chr1.4:74221225-74221244 None:intergenic 35.0%
GGATGGAGTCAAGTTTATAA+AGG - chr1.4:74221274-74221293 MS.gene34020:CDS 35.0%
GTTGGATTTGAACAGTATTC+AGG - chr1.4:74218281-74218300 MS.gene34020:CDS 35.0%
TAACTGGGATTTCATATGCT+TGG - chr1.4:74220304-74220323 MS.gene34020:intron 35.0%
TAATGAGATTATCAAGGCAG+GGG + chr1.4:74221223-74221242 None:intergenic 35.0%
TATTCTACTATCGTTGCAAC+AGG - chr1.4:74219816-74219835 MS.gene34020:intron 35.0%
TCATATGCTCTATACATCCA+AGG + chr1.4:74220213-74220232 None:intergenic 35.0%
TGACACTCGTGATTAAGAAA+TGG + chr1.4:74220811-74220830 None:intergenic 35.0%
TTATGAAGCACAGACAATGA+CGG - chr1.4:74218732-74218751 MS.gene34020:intron 35.0%
! CACAATGCTTCTAAAAGTGA+TGG + chr1.4:74220788-74220807 None:intergenic 35.0%
! CATAATAAGTGTCACGCTTT+TGG + chr1.4:74221085-74221104 None:intergenic 35.0%
! CCAGAGGAAGTATATAGTTA+AGG + chr1.4:74220551-74220570 None:intergenic 35.0%
! TACAGATTCCCTTATTTCTC+TGG + chr1.4:74219695-74219714 None:intergenic 35.0%
! TATAGGAAGCATAGACAATG+GGG + chr1.4:74220474-74220493 None:intergenic 35.0%
! TATAGTTTTCCCCCACTTAT+CGG + chr1.4:74218847-74218866 None:intergenic 35.0%
! TATGGTGCTTCTGAAGAAAT+TGG - chr1.4:74218437-74218456 MS.gene34020:intron 35.0%
! TTCTCTTTTGTGAAGGTTTG+TGG - chr1.4:74221253-74221272 MS.gene34020:CDS 35.0%
!! ATCGCAGATTTTGAAAGGAT+TGG - chr1.4:74221190-74221209 MS.gene34020:CDS 35.0%
!! CTAAACATTGGCTGTGATTT+TGG - chr1.4:74219934-74219953 MS.gene34020:intron 35.0%
!! TAAAATGGTTTTGCCCATGA+AGG - chr1.4:74219349-74219368 MS.gene34020:CDS 35.0%
!!! CCACTAGTTTTATGGCTTAA+TGG - chr1.4:74218389-74218408 MS.gene34020:intron 35.0%
!!! CTAGTTTTATGGCTTAATGG+TGG - chr1.4:74218392-74218411 MS.gene34020:intron 35.0%
!!! TTTTTTTGACTTTGCAGCTG+AGG - chr1.4:74219448-74219467 MS.gene34020:intron 35.0%
AACTTATTAATGCCGGTCTC+CGG - chr1.4:74220501-74220520 MS.gene34020:intron 40.0%
AATACCACATCACTCAGAAG+CGG - chr1.4:74220060-74220079 MS.gene34020:CDS 40.0%
ACAGTTGCAATTACTGAGCA+AGG - chr1.4:74219997-74220016 MS.gene34020:intron 40.0%
ACCTTGCCATTATCATACCA+AGG + chr1.4:74221010-74221029 None:intergenic 40.0%
ACTGAGCAAGGAAACATTGA+TGG - chr1.4:74220009-74220028 MS.gene34020:intron 40.0%
ACTGCGAGTAAATCCTTCAT+GGG + chr1.4:74219365-74219384 None:intergenic 40.0%
AGCTACCAACCATCATCAAT+TGG - chr1.4:74220981-74221000 MS.gene34020:intron 40.0%
ATTCGAGCCATTATCAGTGT+GGG - chr1.4:74221142-74221161 MS.gene34020:CDS 40.0%
CACTTCATGCCAATGTAACT+GGG - chr1.4:74220289-74220308 MS.gene34020:intron 40.0%
CATAGAGAATTCTACATGGC+TGG - chr1.4:74219521-74219540 MS.gene34020:CDS 40.0%
CATATGATCCATGTGCTGAA+AGG - chr1.4:74220226-74220245 MS.gene34020:intron 40.0%
CCTTGCCATTATCATACCAA+GGG + chr1.4:74221009-74221028 None:intergenic 40.0%
CTATCATGTCAAAGGCAGTA+AGG - chr1.4:74221059-74221078 MS.gene34020:intron 40.0%
CTGAAGAACATAATGACCTG+AGG + chr1.4:74219655-74219674 None:intergenic 40.0%
GATGTGGTATTGTTACAAGG+TGG + chr1.4:74220051-74220070 None:intergenic 40.0%
GGTTGTTCTTCCATAGCTTA+TGG - chr1.4:74218419-74218438 MS.gene34020:intron 40.0%
TAAGCTATGGAAGAACAACC+GGG + chr1.4:74218419-74218438 None:intergenic 40.0%
TAGAGAATTCTACATGGCTG+GGG - chr1.4:74219523-74219542 MS.gene34020:CDS 40.0%
TCAGAATACCTTTCAGCACA+TGG + chr1.4:74220237-74220256 None:intergenic 40.0%
TCTTGACAGTGGTACTGTTT+GGG - chr1.4:74220433-74220452 MS.gene34020:intron 40.0%
TGGGATTTCATATGCTTGGA+AGG - chr1.4:74220308-74220327 MS.gene34020:intron 40.0%
TGGTACCCTTGGTATGATAA+TGG - chr1.4:74221001-74221020 MS.gene34020:intron 40.0%
TTCAAGTCCCACACTGATAA+TGG + chr1.4:74221152-74221171 None:intergenic 40.0%
TTCTTGACAGTGGTACTGTT+TGG - chr1.4:74220432-74220451 MS.gene34020:intron 40.0%
! AAGTAAAGTGACTTGCCATC+AGG + chr1.4:74218479-74218498 None:intergenic 40.0%
! ACATTTGGTGACCAGAAAAC+AGG - chr1.4:74218695-74218714 MS.gene34020:intron 40.0%
! AGTTTTACAAGGGGGTCAAT+TGG - chr1.4:74218765-74218784 MS.gene34020:intron 40.0%
! CGACTACATTACCTGTTTTC+TGG + chr1.4:74218709-74218728 None:intergenic 40.0%
! CTTTTGTGAAGGTTTGTGGA+TGG - chr1.4:74221257-74221276 MS.gene34020:CDS 40.0%
! GTGGTACTGTTTGGGATTAT+TGG - chr1.4:74220441-74220460 MS.gene34020:intron 40.0%
! TGGTCTTGGTTAGACACTTT+CGG + chr1.4:74219072-74219091 None:intergenic 40.0%
!! AGTACTAGTGTTGACGTGTT+TGG + chr1.4:74219278-74219297 None:intergenic 40.0%
!! TGGTATGATAATGGCAAGGT+TGG - chr1.4:74221010-74221029 MS.gene34020:intron 40.0%
!! TTCAGAAGCACCATAAGCTA+TGG + chr1.4:74218432-74218451 None:intergenic 40.0%
!! TTGGAACATTTGAGTACTGG+TGG - chr1.4:74219875-74219894 MS.gene34020:CDS 40.0%
!!! GAATTTGTTTTGGGCAGTCA+TGG - chr1.4:74219209-74219228 MS.gene34020:intron 40.0%
!!! GTGGAAGAGCATTGTTTTAC+TGG - chr1.4:74218327-74218346 MS.gene34020:intron 40.0%
AAGTGGGGGAAAACTATACG+CGG - chr1.4:74218849-74218868 MS.gene34020:intron 45.0%
AGGCTATGTGACTGTGAATG+AGG - chr1.4:74218301-74218320 MS.gene34020:intron 45.0%
ATACATCCAAGGCTGAAGAG+AGG + chr1.4:74220202-74220221 None:intergenic 45.0%
CAATCGTCCAGAAGTTCAGA+AGG - chr1.4:74220266-74220285 MS.gene34020:intron 45.0%
CACTGCGAGTAAATCCTTCA+TGG + chr1.4:74219366-74219385 None:intergenic 45.0%
CATTCGAGCCATTATCAGTG+TGG - chr1.4:74221141-74221160 MS.gene34020:CDS 45.0%
CCAGGGCAACCAAAAAATGT+TGG - chr1.4:74218263-74218282 MS.gene34020:CDS 45.0%
CCATCAGGCCTAATACGAAA+AGG + chr1.4:74218464-74218483 None:intergenic 45.0%
CCATCATCAATTGGTACCCT+TGG - chr1.4:74220990-74221009 MS.gene34020:intron 45.0%
CTACTATCGTTGCAACAGGT+TGG - chr1.4:74219820-74219839 MS.gene34020:intron 45.0%
CTCACATTGGTCACAGTAAG+AGG - chr1.4:74221298-74221317 MS.gene34020:CDS 45.0%
GCACTTCATGCCAATGTAAC+TGG - chr1.4:74220288-74220307 MS.gene34020:intron 45.0%
TAAGAAAAGCCAACACCAGC+AGG + chr1.4:74218650-74218669 None:intergenic 45.0%
TACATCCAAGGCTGAAGAGA+GGG + chr1.4:74220201-74220220 None:intergenic 45.0%
TCCATCAGTGCAATGTTTGC+AGG - chr1.4:74219969-74219988 MS.gene34020:intron 45.0%
TTATGGCTTAATGGTGGTCC+CGG - chr1.4:74218398-74218417 MS.gene34020:intron 45.0%
! CCTTTTCGTATTAGGCCTGA+TGG - chr1.4:74218461-74218480 MS.gene34020:intron 45.0%
! CGGTGTCTATTTTGTGCTCA+GGG + chr1.4:74219246-74219265 None:intergenic 45.0%
! GAATATCGAGTTGCAGTCAG+TGG + chr1.4:74220947-74220966 None:intergenic 45.0%
! GGTGTCTATTTTGTGCTCAG+GGG + chr1.4:74219245-74219264 None:intergenic 45.0%
! TGAAGTGCCTTCTGAACTTC+TGG + chr1.4:74220276-74220295 None:intergenic 45.0%
! TTGCACTGATGGATGCTGAA+TGG + chr1.4:74219962-74219981 None:intergenic 45.0%
! TTTTTCCCTCTCTTCAGCCT+TGG - chr1.4:74220193-74220212 MS.gene34020:intron 45.0%
!! CACACTGATAATGGCTCGAA+TGG + chr1.4:74221143-74221162 None:intergenic 45.0%
!! CCAAGGGTACCAATTGATGA+TGG + chr1.4:74220993-74221012 None:intergenic 45.0%
!! CCCTTGGTATGATAATGGCA+AGG - chr1.4:74221006-74221025 MS.gene34020:intron 45.0%
!! GACGTGTTTGGTGTCAATGT+CGG + chr1.4:74219266-74219285 None:intergenic 45.0%
!! GATGATGGTTGGTAGCTTCA+AGG + chr1.4:74220978-74220997 None:intergenic 45.0%
!! GGGTACCAATTGATGATGGT+TGG + chr1.4:74220989-74221008 None:intergenic 45.0%
!! TCGGTGTCTATTTTGTGCTC+AGG + chr1.4:74219247-74219266 None:intergenic 45.0%
!! TTTGAGTACTGGTGGACACA+TGG - chr1.4:74219883-74219902 MS.gene34020:CDS 45.0%
!!! CCAACATTTTTTGGTTGCCC+TGG + chr1.4:74218266-74218285 None:intergenic 45.0%
!!! CTTTCGTATTTTGGCTGCAG+TGG - chr1.4:74220902-74220921 MS.gene34020:CDS 45.0%
AAGCAAGGCGCACATGTATC+TGG + chr1.4:74220622-74220641 None:intergenic 50.0%
ATGGCTGGGGAAAGTTATGC+AGG - chr1.4:74219536-74219555 MS.gene34020:CDS 50.0%
GAATTGGGTCCATGACTCAG+TGG + chr1.4:74218365-74218384 None:intergenic 50.0%
GCCTGCAAACATTGCACTGA+TGG + chr1.4:74219973-74219992 None:intergenic 50.0%
GCTTCACGAGGCTTATGAAG+AGG + chr1.4:74221340-74221359 None:intergenic 50.0%
GGTACCTGTATACCCATGTC+CGG + chr1.4:74220523-74220542 None:intergenic 50.0%
GTGACTGTGAATGAGGAGAG+TGG - chr1.4:74218308-74218327 MS.gene34020:intron 50.0%
! ACTGAAGCACCACTGAGTCA+TGG - chr1.4:74218353-74218372 MS.gene34020:intron 50.0%
! CATATGCTTGGAAGGCATGC+AGG - chr1.4:74220316-74220335 MS.gene34020:intron 50.0%
! TTGGTCACAGTAAGAGGTGC+TGG - chr1.4:74221304-74221323 MS.gene34020:CDS 50.0%
!! TGGTCACAGTAAGAGGTGCT+GGG - chr1.4:74221305-74221324 MS.gene34020:CDS 50.0%
CAAGCCGCTTCTGAGTGATG+TGG + chr1.4:74220067-74220086 None:intergenic 55.0%
CACATGTGTCAGCGTTGCGT+CGG - chr1.4:74219311-74219330 MS.gene34020:CDS 55.0%
GTATACCCATGTCCGGAGAC+CGG + chr1.4:74220516-74220535 None:intergenic 55.0%
GTCTCCGGACATGGGTATAC+AGG - chr1.4:74220516-74220535 MS.gene34020:intron 55.0%
TAATGCCGGTCTCCGGACAT+GGG - chr1.4:74220508-74220527 MS.gene34020:intron 55.0%
TCGACGAATCGAAGCGACGA+GGG + chr1.4:74218556-74218575 None:intergenic 55.0%
TTAATGCCGGTCTCCGGACA+TGG - chr1.4:74220507-74220526 MS.gene34020:intron 55.0%
TTCGACGAATCGAAGCGACG+AGG + chr1.4:74218557-74218576 None:intergenic 55.0%
!! AGTAAGAGGTGCTGGGCATG+AGG - chr1.4:74221312-74221331 MS.gene34020:CDS 55.0%
CAACACCAGCAGGAGACTCG+AGG + chr1.4:74218640-74218659 None:intergenic 60.0%
CGACGCAACGCTGACACATG+TGG + chr1.4:74219313-74219332 None:intergenic 60.0%
CTCGAGTCTCCTGCTGGTGT+TGG - chr1.4:74218638-74218657 MS.gene34020:CDS 60.0%
CTGTTCCTCGAGTCTCCTGC+TGG - chr1.4:74218632-74218651 MS.gene34020:CDS 60.0%
! GCGTTGCGTCGGTGATGCAT+AGG - chr1.4:74219322-74219341 MS.gene34020:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 74218170 74221411 74218170 ID=MS.gene34020
chr1.4 mRNA 74218170 74221411 74218170 ID=MS.gene34020.t1;Parent=MS.gene34020
chr1.4 exon 74221060 74221411 74221060 ID=MS.gene34020.t1.exon1;Parent=MS.gene34020.t1
chr1.4 CDS 74221060 74221411 74221060 ID=cds.MS.gene34020.t1;Parent=MS.gene34020.t1
chr1.4 exon 74220865 74220960 74220865 ID=MS.gene34020.t1.exon2;Parent=MS.gene34020.t1
chr1.4 CDS 74220865 74220960 74220865 ID=cds.MS.gene34020.t1;Parent=MS.gene34020.t1
chr1.4 exon 74220024 74220116 74220024 ID=MS.gene34020.t1.exon3;Parent=MS.gene34020.t1
chr1.4 CDS 74220024 74220116 74220024 ID=cds.MS.gene34020.t1;Parent=MS.gene34020.t1
chr1.4 exon 74219838 74219923 74219838 ID=MS.gene34020.t1.exon4;Parent=MS.gene34020.t1
chr1.4 CDS 74219838 74219923 74219838 ID=cds.MS.gene34020.t1;Parent=MS.gene34020.t1
chr1.4 exon 74219483 74219743 74219483 ID=MS.gene34020.t1.exon5;Parent=MS.gene34020.t1
chr1.4 CDS 74219483 74219743 74219483 ID=cds.MS.gene34020.t1;Parent=MS.gene34020.t1
chr1.4 exon 74219244 74219371 74219244 ID=MS.gene34020.t1.exon6;Parent=MS.gene34020.t1
chr1.4 CDS 74219244 74219371 74219244 ID=cds.MS.gene34020.t1;Parent=MS.gene34020.t1
chr1.4 exon 74219044 74219139 74219044 ID=MS.gene34020.t1.exon7;Parent=MS.gene34020.t1
chr1.4 CDS 74219044 74219139 74219044 ID=cds.MS.gene34020.t1;Parent=MS.gene34020.t1
chr1.4 exon 74218554 74218659 74218554 ID=MS.gene34020.t1.exon8;Parent=MS.gene34020.t1
chr1.4 CDS 74218554 74218659 74218554 ID=cds.MS.gene34020.t1;Parent=MS.gene34020.t1
chr1.4 exon 74218170 74218313 74218170 ID=MS.gene34020.t1.exon9;Parent=MS.gene34020.t1
chr1.4 CDS 74218170 74218313 74218170 ID=cds.MS.gene34020.t1;Parent=MS.gene34020.t1
Gene Sequence

>MS.gene34020

ATGAGTCATTCTATATTCTGTGTTGTTTTGTTTCTGGGAATTTCTTTGGCTTCTTCTATAGAAGAACAAAAGAGAGATAAAATTAAGTGGTTACCAGGGCAACCAAAAAATGTTGGATTTGAACAGTATTCAGGCTATGTGACTGTGAATGAGGAGAGTGGAAGAGCATTGTTTTACTGGTTGACTGAAGCACCACTGAGTCATGGACCCAATTCAAAGCCACTAGTTTTATGGCTTAATGGTGGTCCCGGTTGTTCTTCCATAGCTTATGGTGCTTCTGAAGAAATTGGTCCTTTTCGTATTAGGCCTGATGGCAAGTCACTTTACTTGAATCCTTATGCTTGGAACAATTGTAAGTCAAATTCTCATCTAACGAAATTGAACCCTCGTCGCTTCGATTCGTCGAACTAAAAAATATCGTTACTAATATTTATTATTTTGGATGTTGCAGTGGCAAACATGCTGTTCCTCGAGTCTCCTGCTGGTGTTGGCTTTTCTTATTGTAATAAAACAACAGATATGCAGACATTTGGTGACCAGAAAACAGGTAATGTAGTCGTAATTATGAAGCACAGACAATGACGGAGCTAACATAAGTTTTACAAGGGGGTCAATTGGCCACATATAAAGAAGAATGAATAAACTACATTTAAAAACTAATATATTAAATTTAACCGATAAGTGGGGGAAAACTATACGCGGAAAATTTCAGAAGAATTTTTTTCTTATATAAAATCCAAAATTAAAACTGACAATAACTGTCTAGGAAAGTTGAGCTTGACAACACGTCTAACAGTCAAATTCATCCGTAATTGACTTTGTACCAAACAAACTAACAATGTCTTTTTCAAGGTAAATAGCTGATCTATTCGCAAGAAACACTTTAATTGCTCAAGAGTCCGAAAGTGTCTAACCAAGACCAATAAATTTATTAATCACATGATAATTTTTGGACTTTTCTGTTTGTACTTATTTTTGGCTTATAGTTGATTGGAGTAGTTAAATATATATTATTAGGCTAGATAAATATAAAACTTATGAATTTGTTTTGGGCAGTCATGGCTACGCTACGCCCCTGAGCACAAAATAGACACCGACATTGACACCAAACACGTCAACACTAGTACTAACTGAATGTAGCCACATGTGTCAGCGTTGCGTCGGTGATGCATAGGTTTGTAAAATGGTTTTGCCCATGAAGGATTTACTCGCAGTGCCAGATTTGTTTTGTTTTAGCAATGAGATTTGACGTGTTGATATGTATCTGCGATCTGTAACTTTTTTTGACTTTGCAGCTGAGGATGCATATATTTTTCTAGTTAATTGGTTTGAAAGATTTCCTCAGTACAAACATAGAGAATTCTACATGGCTGGGGAAAGTTATGCAGGTGAATCTATATTTGAAATATCAGTTTTCTACTTATCAATGGAATGGCTTGTACAATGAAGAATTGATCAATTTCTTATAGATTTAAATGATTTCCTCAGGTCATTATGTTCTTCAGTTGGCTCAAATTGTTAACCAGAGAAATAAGGGAATCTGTAATCCAGTTATAAATTTTAAGGGATTTATGGTAAGCTTTTTAGATTCATGTTTAAATCTTTTATGTTCACATAGACAGAACAAAACTAACTTATGATAATCATATTCTACTATCGTTGCAACAGGTTGGAAATAGCGTGATTGATGATTATCATGATTATATTGGAACATTTGAGTACTGGTGGACACATGGTTTGATTTCAGATTCCACATATAAGAAGCTAAACATTGGCTGTGATTTTGGGTCCATTCAGCATCCATCAGTGCAATGTTTGCAGGCTCTTACAGTTGCAATTACTGAGCAAGGAAACATTGATGGATATAGCATTAATACACCACCTTGTAACAATACCACATCACTCAGAAGCGGCTTGCATGATCGTTATGTAAGCCTATATGCTTTCAACTCATTCACATTGCTTACAAAAACCATAACATGTATTAGTAGAAGTGTTTTGATGATTTTTTTTTCTTGTTTCTTTTTTCCCTCTCTTCAGCCTTGGATGTATAGAGCATATGATCCATGTGCTGAAAGGTATTCTGATGTGTACTTCAATCGTCCAGAAGTTCAGAAGGCACTTCATGCCAATGTAACTGGGATTTCATATGCTTGGAAGGCATGCAGGTATATTAATTTTAATTTAGTAAATTGATATGTAACATGTTCTATTGAAGTCTATAATTCTATCATGATTTTGAATTGGAAGGTTTTTTTCTTCTTCTTGACAGTGGTACTGTTTGGGATTATTGGACTGATTCCCCATTGTCTATGCTTCCTATATATCAAGAACTTATTAATGCCGGTCTCCGGACATGGGTATACAGGTACCATCAACCTTAACTATATACTTCCTCTGGTTTTAAATATAAACAAAAAGTTACATTTATATCTGATCCAAATTTATACCAGATACATGTGCGCCTTGCTTGTATTTAAGACTAGAGGGAGTATTTGCGTTTGACTCCTATGAAACGAAATTTCACTTAAAAGTATGAAGCAAGATATATATCTCAACACTTGTCAAAATAATTCATATGATCTAATGTGAATTACTTATTTAAGAGTCTAATCCCATCACTTTTAGAAGCATTGTGCCATTTCTTAATCACGAGTGTCACATATCATGTTTTTATTTCAGCAATCTCATCCATTTCATATTCGGTAAACTAGAACTCGAATTCTTCTGAACTTTCGTATTTTGGCTGCAGTGGAGACACTGATGCTGTAATTCCACTGACTGCAACTCGATATTCTATTGGTGCCTTGAAGCTACCAACCATCATCAATTGGTACCCTTGGTATGATAATGGCAAGGTTGGTGATAACTTCTATCGTGTCGTTTTTTCTATCATGTCAAAGGCAGTAAGGCCAAAAGCGTGACACTTATTATGCCAATATAAAAACTTAATATTGTATTGGAGCTTATCCATTCGAGCCATTATCAGTGTGGGACTTGAATAATCTCATTATCTCATAATCGCAGATTTTGAAAGGATTGGTTATAGGCCCCTGCCTTGATAATCTCATTATCTCATAATTTTCTCTTTTGTGAAGGTTTGTGGATGGAGTCAAGTTTATAAAGGGCTCACATTGGTCACAGTAAGAGGTGCTGGGCATGAGGTTCCTCTTCATAAGCCTCGTGAAGCATTTATTCTTTTTAGATCATTCTTGGAAAACAAGAATATGCCATCAAGTTAA
Protein sequence

>MS.gene34020.t1

MSHSIFCVVLFLGISLASSIEEQKRDKIKWLPGQPKNVGFEQYSGYVTVNEESGRALFYWLTEAPLSHGPNSKPLVLWLNGGPGCSSIAYGASEEIGPFRIRPDGKSLYLNPYAWNNLANMLFLESPAGVGFSYCNKTTDMQTFGDQKTAEDAYIFLVNWFERFPQYKHREFYMAGESYAGHYVLQLAQIVNQRNKGICNPVINFKGFMVGNSVIDDYHDYIGTFEYWWTHGLISDSTYKKLNIGCDFGSIQHPSVQCLQALTVAITEQGNIDGYSINTPPCNNTTSLRSGLHDRYPWMYRAYDPCAERYSDVYFNRPEVQKALHANVTGISYAWKACSGTVWDYWTDSPLSMLPIYQELINAGLRTWVYSGDTDAVIPLTATRYSIGALKLPTIINWYPWYDNGKVCGWSQVYKGLTLVTVRGAGHEVPLHKPREAFILFRSFLENKNMPSS