AlfalfaGEDB | MS.gene34220

M. sativa cultivar XinJiangDaYe / MS.gene34220

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34220.t1	AET02997.1	100	63	0	0	1	63	1	63	4.30E-30	139.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34220.t1	Q96386	66.7	66	18	3	1	63	1	65	7.2e-18	90.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34220.t1	G7LCX2	100.0	63	0	0	1	63	1	63	3.1e-30	139.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049149	MS.gene34220	0.805853	1.09E-49	-1.69E-46
MS.gene049150	MS.gene34220	0.818809	1.61E-52	-1.69E-46
MS.gene049153	MS.gene34220	0.815393	9.46E-52	-1.69E-46
MS.gene049260	MS.gene34220	0.827468	1.54E-54	-1.69E-46
MS.gene049346	MS.gene34220	0.800115	1.68E-48	-1.69E-46
MS.gene049910	MS.gene34220	0.803113	4.07E-49	-1.69E-46
MS.gene050680	MS.gene34220	0.820866	5.47E-53	-1.69E-46
MS.gene050900	MS.gene34220	0.83838	2.98E-57	-1.69E-46
MS.gene051860	MS.gene34220	0.800605	1.33E-48	-1.69E-46
MS.gene051983	MS.gene34220	0.804388	2.21E-49	-1.69E-46
MS.gene052011	MS.gene34220	-0.800403	1.47E-48	-1.69E-46
MS.gene052077	MS.gene34220	0.83004	3.67E-55	-1.69E-46
MS.gene052340	MS.gene34220	0.803976	2.70E-49	-1.69E-46
MS.gene053184	MS.gene34220	0.823798	1.14E-53	-1.69E-46
MS.gene053494	MS.gene34220	0.852279	5.12E-61	-1.69E-46
MS.gene053836	MS.gene34220	0.810503	1.12E-50	-1.69E-46
MS.gene054183	MS.gene34220	0.837589	4.75E-57	-1.69E-46
MS.gene054349	MS.gene34220	0.813332	2.70E-51	-1.69E-46
MS.gene054542	MS.gene34220	0.811949	5.42E-51	-1.69E-46
MS.gene054918	MS.gene34220	0.828223	1.01E-54	-1.69E-46
MS.gene054930	MS.gene34220	0.824259	8.89E-54	-1.69E-46
MS.gene055116	MS.gene34220	0.819213	1.31E-52	-1.69E-46
MS.gene055356	MS.gene34220	0.816889	4.38E-52	-1.69E-46
MS.gene055358	MS.gene34220	0.838565	2.67E-57	-1.69E-46
MS.gene05553	MS.gene34220	0.834352	3.15E-56	-1.69E-46
MS.gene055604	MS.gene34220	0.810496	1.12E-50	-1.69E-46
MS.gene055701	MS.gene34220	0.821386	4.15E-53	-1.69E-46
MS.gene056723	MS.gene34220	0.819135	1.36E-52	-1.69E-46
MS.gene057396	MS.gene34220	0.813122	3.00E-51	-1.69E-46
MS.gene057448	MS.gene34220	0.807771	4.29E-50	-1.69E-46
MS.gene057450	MS.gene34220	0.804526	2.07E-49	-1.69E-46
MS.gene057469	MS.gene34220	0.815214	1.04E-51	-1.69E-46
MS.gene057639	MS.gene34220	0.80824	3.41E-50	-1.69E-46
MS.gene058002	MS.gene34220	0.802691	4.97E-49	-1.69E-46
MS.gene058142	MS.gene34220	0.814352	1.61E-51	-1.69E-46
MS.gene05886	MS.gene34220	0.815405	9.40E-52	-1.69E-46
MS.gene058949	MS.gene34220	0.824045	9.98E-54	-1.69E-46
MS.gene059011	MS.gene34220	0.818787	1.63E-52	-1.69E-46
MS.gene059285	MS.gene34220	0.821375	4.18E-53	-1.69E-46
MS.gene059420	MS.gene34220	0.833489	5.18E-56	-1.69E-46
MS.gene059609	MS.gene34220	0.80921	2.12E-50	-1.69E-46
MS.gene059958	MS.gene34220	0.82931	5.53E-55	-1.69E-46
MS.gene060247	MS.gene34220	0.81438	1.59E-51	-1.69E-46
MS.gene060495	MS.gene34220	0.810224	1.28E-50	-1.69E-46
MS.gene061049	MS.gene34220	0.845415	4.12E-59	-1.69E-46
MS.gene061114	MS.gene34220	-0.832151	1.11E-55	-1.69E-46
MS.gene061159	MS.gene34220	0.819887	9.17E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34220.t1	MTR_8g060850	100.000	63	0	0	1	63	1	63	7.52e-41	127

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34220.t1	AT3G15353	57.576	66	23	2	1	61	1	66	9.98e-18	69.7
MS.gene34220.t1	AT3G15353	56.923	65	23	2	1	61	1	64	5.08e-17	67.8

Find 12 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGCAATCATGCTCAACAGC+TGG	0.507300	8.4:-47765194	None:intergenic
CTTCGTCGAGACTGTTGTCA+TGG	0.555720	8.4:+47765166	MS.gene34220:CDS
ATATATTCTTTAATGGCCGC+AGG	0.559172	8.4:-47765249	None:intergenic
AGAGGAGCACTTACCCGCAC+TGG	0.574771	8.4:-47764874	None:intergenic
GTTGAGCATGATTGCAAGTG+TGG	0.581040	8.4:+47765200	MS.gene34220:CDS
TTCAGGAAGGGAAACAACTA+CGG	0.588697	8.4:+47765003	MS.gene34220:intron
GCGCTGACAAGAGCCAGTGC+GGG	0.597634	8.4:+47764861	MS.gene34220:CDS
ACAAACATGTCAAGCTCATG+CGG	0.602184	8.4:+47764827	None:intergenic
TGCGCTGACAAGAGCCAGTG+CGG	0.611618	8.4:+47764860	MS.gene34220:CDS
ACTTGCACTAACTGCACCTG+CGG	0.656458	8.4:+47765233	MS.gene34220:CDS
CCTCTTCTGAGTCTCGACAA+TGG	0.679042	8.4:-47765031	None:intergenic
CCATTGTCGAGACTCAGAAG+AGG	0.690774	8.4:+47765031	MS.gene34220:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAGGTTGATGTGTATTTTAT+TGG	+	chr8.4:47764909-47764928	MS.gene34220:intron	25.0%
	ATACCATAGCATCATATATG+AGG	-	chr8.4:47764984-47765003	None:intergenic	30.0%
	TACCATAGCATCATATATGA+GGG	-	chr8.4:47764983-47765002	None:intergenic	30.0%
	TATATGATGCTATGGTATTC+AGG	+	chr8.4:47764986-47765005	MS.gene34220:intron	30.0%
	AACCTTACAGTACAAGATAG+AGG	-	chr8.4:47764895-47764914	None:intergenic	35.0%
	ACCATAGCATCATATATGAG+GGG	-	chr8.4:47764982-47765001	None:intergenic	35.0%
	TCGAGGAGTTATTAGAAACA+TGG	-	chr8.4:47765086-47765105	None:intergenic	35.0%
	CTCCTCTATCTTGTACTGTA+AGG	+	chr8.4:47764890-47764909	MS.gene34220:intron	40.0%
	GATGCTATGGTATTCAGGAA+GGG	+	chr8.4:47764991-47765010	MS.gene34220:intron	40.0%
	TCCCCTCATATATGATGCTA+TGG	+	chr8.4:47764978-47764997	MS.gene34220:intron	40.0%
	TGATGCTATGGTATTCAGGA+AGG	+	chr8.4:47764990-47765009	MS.gene34220:intron	40.0%
	TTCAGGAAGGGAAACAACTA+CGG	+	chr8.4:47765003-47765022	MS.gene34220:intron	40.0%
	GTTGAGCATGATTGCAAGTG+TGG	+	chr8.4:47765200-47765219	MS.gene34220:CDS	45.0%
	TTGCAATCATGCTCAACAGC+TGG	-	chr8.4:47765197-47765216	None:intergenic	45.0%
	ACTTGCACTAACTGCACCTG+CGG	+	chr8.4:47765233-47765252	MS.gene34220:CDS	50.0%
	CCATTGTCGAGACTCAGAAG+AGG	+	chr8.4:47765031-47765050	MS.gene34220:CDS	50.0%
	CCTCTTCTGAGTCTCGACAA+TGG	-	chr8.4:47765034-47765053	None:intergenic	50.0%
	CTTCGTCGAGACTGTTGTCA+TGG	+	chr8.4:47765166-47765185	MS.gene34220:CDS	50.0%
!!	GCTAGCTTTGCATTGAGTCG+AGG	-	chr8.4:47765103-47765122	None:intergenic	50.0%
!	TGCGCTGACAAGAGCCAGTG+CGG	+	chr8.4:47764860-47764879	MS.gene34220:CDS	60.0%
!!	AGAGGAGCACTTACCCGCAC+TGG	-	chr8.4:47764877-47764896	None:intergenic	60.0%
!	GCGCTGACAAGAGCCAGTGC+GGG	+	chr8.4:47764861-47764880	MS.gene34220:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	47764833	47765262	47764833	ID=MS.gene34220
chr8.4	mRNA	47764833	47765262	47764833	ID=MS.gene34220.t1;Parent=MS.gene34220
chr8.4	exon	47764833	47764882	47764833	ID=MS.gene34220.t1.exon1;Parent=MS.gene34220.t1
chr8.4	CDS	47764833	47764882	47764833	ID=cds.MS.gene34220.t1;Parent=MS.gene34220.t1
chr8.4	exon	47765008	47765052	47765008	ID=MS.gene34220.t1.exon2;Parent=MS.gene34220.t1
chr8.4	CDS	47765008	47765052	47765008	ID=cds.MS.gene34220.t1;Parent=MS.gene34220.t1
chr8.4	exon	47765166	47765262	47765166	ID=MS.gene34220.t1.exon3;Parent=MS.gene34220.t1
chr8.4	CDS	47765166	47765262	47765166	ID=cds.MS.gene34220.t1;Parent=MS.gene34220.t1

Gene Sequence

>MS.gene34220

ATGTCAAGCTCATGCGGCAACTGCGACTGCGCTGACAAGAGCCAGTGCGGGTAAGTGCTCCTCTATCTTGTACTGTAAGGTTGATGTGTATTTTATTGGAGTGTATTTCAAAACAGCGAATAGAACATTTGAATTAACTATATTCTCCCCTCATATATGATGCTATGGTATTCAGGAAGGGAAACAACTACGGCATGACCATTGTCGAGACTCAGAAGAGGTAAAAGTTTCTTTTTGATGTTCAATGTTTCCATGTTTCTAATAACTCCTCGACTCAATGCAAAGCTAGCTGAATACAGCAACAACATAATGATTGTCAATTGTTTTGTGCAGCTTCGTCGAGACTGTTGTCATGGATGCTCCAGCTGTTGAGCATGATTGCAAGTGTGGTTCTAACTGCACTTGCACTAACTGCACCTGCGGCCATTAA

Protein sequence

>MS.gene34220.t1

MSSSCGNCDCADKSQCGKGNNYGMTIVETQKSFVETVVMDAPAVEHDCKCGSNCTCTNCTCGH