AlfalfaGEDB | MS.gene36353

M. sativa cultivar XinJiangDaYe / MS.gene36353

KEGG: -
GO: -
PFAM: PF07911.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36353.t1	XP_003597686.1	99	196	2	0	1	196	1	196	3.30E-105	391

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36353.t1	G7II08	99.0	196	2	0	1	196	1	196	2.4e-105	391.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049356	MS.gene36353	0.872376	3.33E-67	-1.69E-46
MS.gene049369	MS.gene36353	0.84623	2.48E-59	-1.69E-46
MS.gene049396	MS.gene36353	0.822086	2.86E-53	-1.69E-46
MS.gene050128	MS.gene36353	0.819254	1.28E-52	-1.69E-46
MS.gene050670	MS.gene36353	0.829685	4.48E-55	-1.69E-46
MS.gene050971	MS.gene36353	0.80786	4.11E-50	-1.69E-46
MS.gene05105	MS.gene36353	0.815434	9.26E-52	-1.69E-46
MS.gene051360	MS.gene36353	0.830048	3.66E-55	-1.69E-46
MS.gene051605	MS.gene36353	0.807247	5.55E-50	-1.69E-46
MS.gene052188	MS.gene36353	0.856166	3.86E-62	-1.69E-46
MS.gene052253	MS.gene36353	0.818559	1.84E-52	-1.69E-46
MS.gene052340	MS.gene36353	0.839743	1.32E-57	-1.69E-46
MS.gene052485	MS.gene36353	0.827569	1.46E-54	-1.69E-46
MS.gene053169	MS.gene36353	0.812106	5.01E-51	-1.69E-46
MS.gene05316	MS.gene36353	0.858846	6.22E-63	-1.69E-46
MS.gene053449	MS.gene36353	0.824534	7.66E-54	-1.69E-46
MS.gene053758	MS.gene36353	0.833697	4.59E-56	-1.69E-46
MS.gene053813	MS.gene36353	0.831959	1.24E-55	-1.69E-46
MS.gene053912	MS.gene36353	0.832982	6.93E-56	-1.69E-46
MS.gene054556	MS.gene36353	0.808584	2.88E-50	-1.69E-46
MS.gene055116	MS.gene36353	0.838686	2.48E-57	-1.69E-46
MS.gene055666	MS.gene36353	0.808989	2.36E-50	-1.69E-46
MS.gene055909	MS.gene36353	0.842818	2.05E-58	-1.69E-46
MS.gene055911	MS.gene36353	0.840728	7.30E-58	-1.69E-46
MS.gene056422	MS.gene36353	0.840863	6.73E-58	-1.69E-46
MS.gene056511	MS.gene36353	0.854406	1.26E-61	-1.69E-46
MS.gene056659	MS.gene36353	0.808714	2.70E-50	-1.69E-46
MS.gene057396	MS.gene36353	0.826108	3.25E-54	-1.69E-46
MS.gene057448	MS.gene36353	0.848396	6.29E-60	-1.69E-46
MS.gene057449	MS.gene36353	0.856328	3.46E-62	-1.69E-46
MS.gene057450	MS.gene36353	0.848355	6.46E-60	-1.69E-46
MS.gene057537	MS.gene36353	0.823446	1.38E-53	-1.69E-46
MS.gene057610	MS.gene36353	0.819232	1.29E-52	-1.69E-46
MS.gene057734	MS.gene36353	0.807531	4.83E-50	-1.69E-46
MS.gene058007	MS.gene36353	0.857125	2.02E-62	-1.69E-46
MS.gene058075	MS.gene36353	0.827847	1.25E-54	-1.69E-46
MS.gene05842	MS.gene36353	0.806637	7.47E-50	-1.69E-46
MS.gene058459	MS.gene36353	0.803245	3.82E-49	-1.69E-46
MS.gene058700	MS.gene36353	0.80723	5.60E-50	-1.69E-46
MS.gene058928	MS.gene36353	0.836386	9.64E-57	-1.69E-46
MS.gene058949	MS.gene36353	0.821033	5.01E-53	-1.69E-46
MS.gene058995	MS.gene36353	0.803208	3.89E-49	-1.69E-46
MS.gene05907	MS.gene36353	0.802443	5.60E-49	-1.69E-46
MS.gene059080	MS.gene36353	0.820547	6.47E-53	-1.69E-46
MS.gene059082	MS.gene36353	0.833259	5.91E-56	-1.69E-46
MS.gene059294	MS.gene36353	0.813263	2.80E-51	-1.69E-46
MS.gene059366	MS.gene36353	0.839161	1.87E-57	-1.69E-46
MS.gene059420	MS.gene36353	0.81142	7.06E-51	-1.69E-46
MS.gene059462	MS.gene36353	0.838494	2.78E-57	-1.69E-46
MS.gene059674	MS.gene36353	0.853249	2.71E-61	-1.69E-46
MS.gene059688	MS.gene36353	0.845002	5.33E-59	-1.69E-46
MS.gene059692	MS.gene36353	0.829572	4.78E-55	-1.69E-46
MS.gene059695	MS.gene36353	0.825404	4.78E-54	-1.69E-46
MS.gene059711	MS.gene36353	0.840078	1.08E-57	-1.69E-46
MS.gene059807	MS.gene36353	0.897214	1.75E-76	-1.69E-46
MS.gene059876	MS.gene36353	0.809955	1.47E-50	-1.69E-46
MS.gene060247	MS.gene36353	0.804241	2.37E-49	-1.69E-46
MS.gene060372	MS.gene36353	0.803559	3.29E-49	-1.69E-46
MS.gene060495	MS.gene36353	0.823008	1.75E-53	-1.69E-46
MS.gene060837	MS.gene36353	0.836634	8.34E-57	-1.69E-46
MS.gene061051	MS.gene36353	-0.819592	1.07E-52	-1.69E-46
MS.gene061154	MS.gene36353	0.802533	5.36E-49	-1.69E-46
MS.gene061159	MS.gene36353	0.814876	1.23E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36353.t1	MTR_2g101140	98.980	196	2	0	1	196	1	196	3.08e-145	402
MS.gene36353.t1	MTR_8g013990	67.839	199	54	5	1	196	3	194	6.77e-92	267
MS.gene36353.t1	MTR_8g021190	42.969	128	69	3	61	186	29	154	2.34e-29	106
MS.gene36353.t1	MTR_1g103550	47.191	89	41	2	62	148	2	86	2.72e-22	87.8
MS.gene36353.t1	MTR_5g006430	38.053	113	65	2	62	173	2	110	1.50e-20	82.8
MS.gene36353.t1	MTR_3g105340	52.778	72	33	1	52	122	1	72	2.76e-20	82.8
MS.gene36353.t1	MTR_3g109860	34.959	123	77	3	32	151	4	126	1.51e-17	75.9
MS.gene36353.t1	MTR_4g125010	50.725	69	33	1	61	128	23	91	6.19e-17	74.7
MS.gene36353.t1	MTR_4g125030	42.708	96	47	3	61	151	21	113	3.68e-16	71.6
MS.gene36353.t1	MTR_1g014930	41.111	90	39	3	53	135	4	86	2.21e-15	70.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36353.t1	AT5G25840	50.276	181	59	4	36	196	4	173	3.66e-53	168
MS.gene36353.t1	AT1G79770	53.205	156	59	3	35	190	26	167	1.23e-48	156
MS.gene36353.t1	AT1G72510	33.537	164	90	5	39	186	5	165	8.76e-23	90.1
MS.gene36353.t1	AT1G72510	33.537	164	90	5	39	186	5	165	8.76e-23	90.1
MS.gene36353.t1	AT4G14819	40.777	103	50	3	62	164	2	93	5.28e-22	86.3
MS.gene36353.t1	AT3G22540	40.449	89	50	1	62	150	2	87	1.92e-21	85.1
MS.gene36353.t1	AT2G25780	36.250	160	88	5	31	185	1	151	1.10e-20	84.3
MS.gene36353.t1	AT2G09970	31.707	164	92	5	40	186	6	166	1.23e-20	84.7
MS.gene36353.t1	AT1G54095	37.410	139	75	4	61	189	14	150	5.47e-20	82.4
MS.gene36353.t1	AT5G20670	33.846	130	80	3	63	186	23	152	3.57e-18	77.8

Find 34 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTGTTAGATTTAATAAATT+TGG	0.209428	2.2:+2263648	MS.gene36353:CDS
AGCTTCTATATAAGATTAAT+TGG	0.236977	2.2:-2263859	None:intergenic
GCATCTTCAACTTCAGTTAT+CGG	0.334901	2.2:-2263450	None:intergenic
GTGATAAATACTTAGGGAAA+TGG	0.349825	2.2:+2263532	MS.gene36353:CDS
AGTTGAGATGAATTAGGTTT+TGG	0.358947	2.2:-2263345	None:intergenic
GCTTCAGCTTGAAACAAAAC+AGG	0.390939	2.2:-2263681	None:intergenic
CGTGTACGTGATAAATACTT+AGG	0.397786	2.2:+2263525	MS.gene36353:CDS
TTGTTGAGTTGAGATGAATT+AGG	0.428907	2.2:-2263351	None:intergenic
TTTCACCACCTTTGTCTCTA+GGG	0.429554	2.2:-2263761	None:intergenic
TGATAAATACTTAGGGAAAT+GGG	0.445526	2.2:+2263533	MS.gene36353:CDS
TGAGGCAGTGAAAGAAGAGT+TGG	0.465910	2.2:+2263572	MS.gene36353:CDS
TTTGGAGCATACAATGTTGT+TGG	0.466523	2.2:-2263319	None:intergenic
TACTTAGGGAAATGGGTTTG+TGG	0.466697	2.2:+2263540	MS.gene36353:CDS
GAGTAAAATGGAAGGGAGAA+GGG	0.485393	2.2:+2263725	MS.gene36353:CDS
TTGATATCTCTTGTAATAGC+AGG	0.500356	2.2:-2263822	None:intergenic
AGAGTAAAATGGAAGGGAGA+AGG	0.512738	2.2:+2263724	MS.gene36353:CDS
GTGTACGTGATAAATACTTA+GGG	0.514569	2.2:+2263526	MS.gene36353:CDS
TCAACTATTATGGCACCTCA+TGG	0.537712	2.2:+2263267	None:intergenic
GTGGAAGCTGTGTTAAATCA+AGG	0.538899	2.2:-2263428	None:intergenic
GGTGGTGAAAAGAAAGGTGG+TGG	0.545436	2.2:+2263774	MS.gene36353:CDS
TTAGATTTAATAAATTTGGA+AGG	0.546403	2.2:+2263652	MS.gene36353:CDS
CATTACCTAGCTCAATCTCT+AGG	0.548665	2.2:+2263295	MS.gene36353:CDS
GACAAAGGTGGTGAAAAGAA+AGG	0.558067	2.2:+2263768	MS.gene36353:CDS
TTGGTCCTAGAGATTGAGCT+AGG	0.570237	2.2:-2263300	None:intergenic
GGTTTGTGGTTTGTGTTCTG+AGG	0.597680	2.2:+2263554	MS.gene36353:CDS
TGGTGGGGAAAATATTGAAG+AGG	0.600750	2.2:+2263602	MS.gene36353:CDS
TCTTCAACTTCAGTTATCGG+TGG	0.626778	2.2:-2263447	None:intergenic
TCATTCAACCCTAGAGACAA+AGG	0.638410	2.2:+2263753	MS.gene36353:CDS
CTTCAGCTTGAAACAAAACA+GGG	0.640341	2.2:-2263680	None:intergenic
GTGGTGAAAAGAAAGGTGGT+GGG	0.641104	2.2:+2263775	MS.gene36353:CDS
AAAGGTGGTGAAAAGAAAGG+TGG	0.652842	2.2:+2263771	MS.gene36353:CDS
GATGCAAAGTGTGAATGTTG+TGG	0.690241	2.2:+2263468	MS.gene36353:CDS
TTCAACCCTAGAGACAAAGG+TGG	0.741656	2.2:+2263756	MS.gene36353:CDS
CTAGGTAATGAATCTCCATG+AGG	0.751297	2.2:-2263282	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGTGTTAGATTTAATAAATT+TGG	+	chr2.2:2263648-2263667	MS.gene36353:CDS	15.0%
!!	TTAGATTTAATAAATTTGGA+AGG	+	chr2.2:2263652-2263671	MS.gene36353:CDS	15.0%
!	TGAAAAAGAGTAAAATGGAA+GGG	+	chr2.2:2263718-2263737	MS.gene36353:CDS	25.0%
!	TGATAAATACTTAGGGAAAT+GGG	+	chr2.2:2263533-2263552	MS.gene36353:CDS	25.0%
	AAAGAAGAGTTGGAGAAAAA+TGG	+	chr2.2:2263582-2263601	MS.gene36353:CDS	30.0%
	CTGAAAAAGAGTAAAATGGA+AGG	+	chr2.2:2263717-2263736	MS.gene36353:CDS	30.0%
	GATGCTGAAAAAGAGTAAAA+TGG	+	chr2.2:2263713-2263732	MS.gene36353:CDS	30.0%
	GTGATAAATACTTAGGGAAA+TGG	+	chr2.2:2263532-2263551	MS.gene36353:CDS	30.0%
	GTGTACGTGATAAATACTTA+GGG	+	chr2.2:2263526-2263545	MS.gene36353:CDS	30.0%
	TTGATATCTCTTGTAATAGC+AGG	-	chr2.2:2263825-2263844	None:intergenic	30.0%
!	AGTTGAGATGAATTAGGTTT+TGG	-	chr2.2:2263348-2263367	None:intergenic	30.0%
!!	TTGTTGAGTTGAGATGAATT+AGG	-	chr2.2:2263354-2263373	None:intergenic	30.0%
	AAGAGTTGGAGAAAAATGGT+GGG	+	chr2.2:2263586-2263605	MS.gene36353:CDS	35.0%
	CGTGTACGTGATAAATACTT+AGG	+	chr2.2:2263525-2263544	MS.gene36353:CDS	35.0%
	CTTCAGCTTGAAACAAAACA+GGG	-	chr2.2:2263683-2263702	None:intergenic	35.0%
	GCATCTTCAACTTCAGTTAT+CGG	-	chr2.2:2263453-2263472	None:intergenic	35.0%
!	ACGTACACGTTTTATGTACT+CGG	-	chr2.2:2263514-2263533	None:intergenic	35.0%
!	TTTGGAGCATACAATGTTGT+TGG	-	chr2.2:2263322-2263341	None:intergenic	35.0%
!!	TGAATTAGGTTTTGGTGGTT+TGG	-	chr2.2:2263340-2263359	None:intergenic	35.0%
!!	TGAGATGAATTAGGTTTTGG+TGG	-	chr2.2:2263345-2263364	None:intergenic	35.0%
	AAAGGTGGTGAAAAGAAAGG+TGG	+	chr2.2:2263771-2263790	MS.gene36353:CDS	40.0%
	AGAGTAAAATGGAAGGGAGA+AGG	+	chr2.2:2263724-2263743	MS.gene36353:CDS	40.0%
	AGAGTTGGAGAAAAATGGTG+GGG	+	chr2.2:2263587-2263606	MS.gene36353:CDS	40.0%
	CATTACCTAGCTCAATCTCT+AGG	+	chr2.2:2263295-2263314	MS.gene36353:CDS	40.0%
	CTAGGTAATGAATCTCCATG+AGG	-	chr2.2:2263285-2263304	None:intergenic	40.0%
	GAAGAGTTGGAGAAAAATGG+TGG	+	chr2.2:2263585-2263604	MS.gene36353:CDS	40.0%
	GACAAAGGTGGTGAAAAGAA+AGG	+	chr2.2:2263768-2263787	MS.gene36353:CDS	40.0%
	GAGTAAAATGGAAGGGAGAA+GGG	+	chr2.2:2263725-2263744	MS.gene36353:CDS	40.0%
	GATGCAAAGTGTGAATGTTG+TGG	+	chr2.2:2263468-2263487	MS.gene36353:CDS	40.0%
	GCTTCAGCTTGAAACAAAAC+AGG	-	chr2.2:2263684-2263703	None:intergenic	40.0%
	GTGGAAGCTGTGTTAAATCA+AGG	-	chr2.2:2263431-2263450	None:intergenic	40.0%
	TCATTCAACCCTAGAGACAA+AGG	+	chr2.2:2263753-2263772	MS.gene36353:CDS	40.0%
	TCTTCAACTTCAGTTATCGG+TGG	-	chr2.2:2263450-2263469	None:intergenic	40.0%
	TGGTGGGGAAAATATTGAAG+AGG	+	chr2.2:2263602-2263621	MS.gene36353:CDS	40.0%
	TTTCACCACCTTTGTCTCTA+GGG	-	chr2.2:2263764-2263783	None:intergenic	40.0%
!	CGTACACGTTTTATGTACTC+GGG	-	chr2.2:2263513-2263532	None:intergenic	40.0%
!	TACTTAGGGAAATGGGTTTG+TGG	+	chr2.2:2263540-2263559	MS.gene36353:CDS	40.0%
!	TTTTCACCACCTTTGTCTCT+AGG	-	chr2.2:2263765-2263784	None:intergenic	40.0%
	GTGGTGAAAAGAAAGGTGGT+GGG	+	chr2.2:2263775-2263794	MS.gene36353:CDS	45.0%
	TGAGGCAGTGAAAGAAGAGT+TGG	+	chr2.2:2263572-2263591	MS.gene36353:CDS	45.0%
	TTCAACCCTAGAGACAAAGG+TGG	+	chr2.2:2263756-2263775	MS.gene36353:CDS	45.0%
	TTGGTCCTAGAGATTGAGCT+AGG	-	chr2.2:2263303-2263322	None:intergenic	45.0%
!	GGTTTGTGGTTTGTGTTCTG+AGG	+	chr2.2:2263554-2263573	MS.gene36353:CDS	45.0%
	GGTGGTGAAAAGAAAGGTGG+TGG	+	chr2.2:2263774-2263793	MS.gene36353:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	2263276	2263866	2263276	ID=MS.gene36353
chr2.2	mRNA	2263276	2263866	2263276	ID=MS.gene36353.t1;Parent=MS.gene36353
chr2.2	exon	2263276	2263866	2263276	ID=MS.gene36353.t1.exon1;Parent=MS.gene36353.t1
chr2.2	CDS	2263276	2263866	2263276	ID=cds.MS.gene36353.t1;Parent=MS.gene36353.t1

Gene Sequence

>MS.gene36353

ATGGCACCTCATGGAGATTCATTACCTAGCTCAATCTCTAGGACCAACAACATTGTATGCTCCAAACCACCAAAACCTAATTCATCTCAACTCAACAACAATAATATTCAAAGAACAATCTCAGACATATCATTTGAACTATCAAAACAAGACCTTGATTTAACACAGCTTCCACCGATAACTGAAGTTGAAGATGCAAAGTGTGAATGTTGTGGCATGTCTGAAGAGTTTACACCCGAGTACATAAAACGTGTACGTGATAAATACTTAGGGAAATGGGTTTGTGGTTTGTGTTCTGAGGCAGTGAAAGAAGAGTTGGAGAAAAATGGTGGGGAAAATATTGAAGAGGCTTTGAGTGCACACATGAGTGCATGTGTTAGATTTAATAAATTTGGAAGGAGTTTCCCTGTTTTGTTTCAAGCTGAAGCTATGAAAGAGATGCTGAAAAAGAGTAAAATGGAAGGGAGAAGGGCTAAGTCATTCAACCCTAGAGACAAAGGTGGTGAAAAGAAAGGTGGTGGGCTTGCTAGAAGTTCAAGTTGCATTCCTGCTATTACAAGAGATATCAATGATTTCACAATCACCAATTAA

Protein sequence

>MS.gene36353.t1

MAPHGDSLPSSISRTNNIVCSKPPKPNSSQLNNNNIQRTISDISFELSKQDLDLTQLPPITEVEDAKCECCGMSEEFTPEYIKRVRDKYLGKWVCGLCSEAVKEELEKNGGENIEEALSAHMSACVRFNKFGRSFPVLFQAEAMKEMLKKSKMEGRRAKSFNPRDKGGEKKGGGLARSSSCIPAITRDINDFTITN