Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36775.t1 | XP_003627006.2 | 95.4 | 195 | 8 | 1 | 1 | 195 | 1 | 194 | 7.90E-99 | 369.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36775.t1 | G7LFY6 | 95.4 | 195 | 8 | 1 | 1 | 195 | 1 | 194 | 5.7e-99 | 369.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049356 | MS.gene36775 | 0.809001 | 2.35E-50 | -1.69E-46 |
| MS.gene050060 | MS.gene36775 | 0.8036 | 3.23E-49 | -1.69E-46 |
| MS.gene050400 | MS.gene36775 | 0.805662 | 1.20E-49 | -1.69E-46 |
| MS.gene050670 | MS.gene36775 | 0.839997 | 1.13E-57 | -1.69E-46 |
| MS.gene050971 | MS.gene36775 | 0.813663 | 2.28E-51 | -1.69E-46 |
| MS.gene051525 | MS.gene36775 | 0.835218 | 1.91E-56 | -1.69E-46 |
| MS.gene052077 | MS.gene36775 | 0.807585 | 4.70E-50 | -1.69E-46 |
| MS.gene052188 | MS.gene36775 | 0.837686 | 4.49E-57 | -1.69E-46 |
| MS.gene052253 | MS.gene36775 | 0.809572 | 1.77E-50 | -1.69E-46 |
| MS.gene052310 | MS.gene36775 | 0.802111 | 6.55E-49 | -1.69E-46 |
| MS.gene052340 | MS.gene36775 | 0.834577 | 2.77E-56 | -1.69E-46 |
| MS.gene052421 | MS.gene36775 | 0.802472 | 5.52E-49 | -1.69E-46 |
| MS.gene052485 | MS.gene36775 | 0.803667 | 3.13E-49 | -1.69E-46 |
| MS.gene05316 | MS.gene36775 | 0.833369 | 5.55E-56 | -1.69E-46 |
| MS.gene053449 | MS.gene36775 | 0.813969 | 1.96E-51 | -1.69E-46 |
| MS.gene053758 | MS.gene36775 | 0.819153 | 1.35E-52 | -1.69E-46 |
| MS.gene053928 | MS.gene36775 | 0.832961 | 7.01E-56 | -1.69E-46 |
| MS.gene054183 | MS.gene36775 | 0.810901 | 9.15E-51 | -1.69E-46 |
| MS.gene054349 | MS.gene36775 | 0.832256 | 1.05E-55 | -1.69E-46 |
| MS.gene055116 | MS.gene36775 | 0.823083 | 1.68E-53 | -1.69E-46 |
| MS.gene055369 | MS.gene36775 | 0.815098 | 1.10E-51 | -1.69E-46 |
| MS.gene05553 | MS.gene36775 | 0.837542 | 4.89E-57 | -1.69E-46 |
| MS.gene055909 | MS.gene36775 | 0.810381 | 1.19E-50 | -1.69E-46 |
| MS.gene055911 | MS.gene36775 | 0.817342 | 3.47E-52 | -1.69E-46 |
| MS.gene056191 | MS.gene36775 | 0.804957 | 1.68E-49 | -1.69E-46 |
| MS.gene056511 | MS.gene36775 | 0.806346 | 8.60E-50 | -1.69E-46 |
| MS.gene056514 | MS.gene36775 | 0.804046 | 2.61E-49 | -1.69E-46 |
| MS.gene057124 | MS.gene36775 | 0.803736 | 3.02E-49 | -1.69E-46 |
| MS.gene057396 | MS.gene36775 | 0.82864 | 8.03E-55 | -1.69E-46 |
| MS.gene057448 | MS.gene36775 | 0.819887 | 9.17E-53 | -1.69E-46 |
| MS.gene057449 | MS.gene36775 | 0.808691 | 2.74E-50 | -1.69E-46 |
| MS.gene057450 | MS.gene36775 | 0.816423 | 5.57E-52 | -1.69E-46 |
| MS.gene057749 | MS.gene36775 | 0.820436 | 6.87E-53 | -1.69E-46 |
| MS.gene057823 | MS.gene36775 | 0.819057 | 1.42E-52 | -1.69E-46 |
| MS.gene058002 | MS.gene36775 | 0.802812 | 4.70E-49 | -1.69E-46 |
| MS.gene058007 | MS.gene36775 | 0.835314 | 1.80E-56 | -1.69E-46 |
| MS.gene05842 | MS.gene36775 | 0.814436 | 1.54E-51 | -1.69E-46 |
| MS.gene058700 | MS.gene36775 | 0.811744 | 6.01E-51 | -1.69E-46 |
| MS.gene05886 | MS.gene36775 | 0.825428 | 4.71E-54 | -1.69E-46 |
| MS.gene058928 | MS.gene36775 | 0.813359 | 2.66E-51 | -1.69E-46 |
| MS.gene05907 | MS.gene36775 | 0.835057 | 2.09E-56 | -1.69E-46 |
| MS.gene059082 | MS.gene36775 | 0.803932 | 2.75E-49 | -1.69E-46 |
| MS.gene059366 | MS.gene36775 | 0.802179 | 6.34E-49 | -1.69E-46 |
| MS.gene059420 | MS.gene36775 | 0.838484 | 2.80E-57 | -1.69E-46 |
| MS.gene059462 | MS.gene36775 | 0.820183 | 7.85E-53 | -1.69E-46 |
| MS.gene059674 | MS.gene36775 | 0.836758 | 7.76E-57 | -1.69E-46 |
| MS.gene059688 | MS.gene36775 | 0.806807 | 6.88E-50 | -1.69E-46 |
| MS.gene059807 | MS.gene36775 | 0.833223 | 6.03E-56 | -1.69E-46 |
| MS.gene060247 | MS.gene36775 | 0.806656 | 7.40E-50 | -1.69E-46 |
| MS.gene061049 | MS.gene36775 | 0.820374 | 7.10E-53 | -1.69E-46 |
| MS.gene061154 | MS.gene36775 | 0.829973 | 3.81E-55 | -1.69E-46 |
| MS.gene061159 | MS.gene36775 | 0.823592 | 1.28E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36775.t1 | MTR_8g013990 | 95.385 | 195 | 8 | 1 | 1 | 195 | 1 | 194 | 6.80e-137 | 380 |
| MS.gene36775.t1 | MTR_2g101140 | 66.332 | 199 | 58 | 5 | 3 | 195 | 1 | 196 | 2.37e-84 | 248 |
| MS.gene36775.t1 | MTR_8g021190 | 46.212 | 132 | 58 | 5 | 61 | 185 | 29 | 154 | 4.28e-31 | 111 |
| MS.gene36775.t1 | MTR_1g103550 | 47.778 | 90 | 39 | 3 | 62 | 148 | 2 | 86 | 3.48e-23 | 90.1 |
| MS.gene36775.t1 | MTR_3g105340 | 59.091 | 66 | 26 | 1 | 59 | 123 | 8 | 73 | 3.74e-22 | 87.4 |
| MS.gene36775.t1 | MTR_5g006430 | 43.750 | 96 | 45 | 3 | 62 | 154 | 2 | 91 | 2.34e-21 | 84.7 |
| MS.gene36775.t1 | MTR_3g109860 | 34.211 | 152 | 87 | 4 | 31 | 179 | 4 | 145 | 6.13e-19 | 79.7 |
| MS.gene36775.t1 | MTR_4g125030 | 47.222 | 72 | 37 | 1 | 53 | 123 | 13 | 84 | 1.43e-17 | 75.1 |
| MS.gene36775.t1 | MTR_4g125010 | 37.234 | 94 | 52 | 2 | 62 | 148 | 24 | 117 | 1.53e-17 | 76.3 |
| MS.gene36775.t1 | MTR_1g014930 | 40.217 | 92 | 47 | 2 | 47 | 134 | 1 | 88 | 9.84e-16 | 71.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36775.t1 | AT5G25840 | 50.543 | 184 | 54 | 5 | 35 | 195 | 4 | 173 | 2.52e-53 | 168 |
| MS.gene36775.t1 | AT1G79770 | 52.903 | 155 | 59 | 3 | 35 | 189 | 27 | 167 | 1.26e-46 | 151 |
| MS.gene36775.t1 | AT4G14819 | 42.727 | 110 | 51 | 3 | 62 | 171 | 2 | 99 | 1.70e-23 | 90.1 |
| MS.gene36775.t1 | AT2G25780 | 36.774 | 155 | 83 | 4 | 35 | 184 | 7 | 151 | 2.23e-22 | 88.6 |
| MS.gene36775.t1 | AT3G22540 | 40.449 | 89 | 50 | 1 | 62 | 150 | 2 | 87 | 2.19e-21 | 84.7 |
| MS.gene36775.t1 | AT1G72510 | 39.706 | 136 | 67 | 7 | 62 | 185 | 33 | 165 | 1.19e-20 | 84.7 |
| MS.gene36775.t1 | AT1G72510 | 39.706 | 136 | 67 | 7 | 62 | 185 | 33 | 165 | 1.19e-20 | 84.7 |
| MS.gene36775.t1 | AT1G54095 | 39.706 | 136 | 69 | 5 | 61 | 185 | 14 | 147 | 3.25e-19 | 80.5 |
| MS.gene36775.t1 | AT5G20670 | 33.582 | 134 | 82 | 3 | 59 | 185 | 19 | 152 | 4.36e-19 | 80.1 |
| MS.gene36775.t1 | AT2G09970 | 34.058 | 138 | 73 | 7 | 62 | 185 | 33 | 166 | 3.97e-16 | 72.8 |
Find 41 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTAACATGGATGGGAAAATT+AGG | 0.194429 | 8.2:-79038446 | MS.gene36775:CDS |
| CCTCCTCCTTTCTTGTCCCT+TGG | 0.275546 | 8.2:+79038408 | None:intergenic |
| AAAATAGATAGATAGTTAAT+TGG | 0.284549 | 8.2:+79038317 | None:intergenic |
| TGTGTTAAGTTTAACAAATA+TGG | 0.300385 | 8.2:-79038525 | MS.gene36775:CDS |
| TATATTTCTTATCATGGTTA+TGG | 0.301068 | 8.2:-79038910 | None:intergenic |
| GAAAGCTTTGTTGGTTTGTT+TGG | 0.348037 | 8.2:+79038831 | None:intergenic |
| GCTTGTGCTTGAAACAAAAC+TGG | 0.379794 | 8.2:+79038492 | None:intergenic |
| GACAAGAAAGGAGGAGGAGG+AGG | 0.400333 | 8.2:-79038402 | MS.gene36775:CDS |
| TCGGAAGTTGAAAGCTTTGT+TGG | 0.400947 | 8.2:+79038822 | None:intergenic |
| TAACATGGATGGGAAAATTA+GGG | 0.434099 | 8.2:-79038445 | MS.gene36775:CDS |
| CGACATTTCCTTCGAGCTGA+TGG | 0.460178 | 8.2:-79038778 | MS.gene36775:CDS |
| TTGATCTCTCTTGTAAGTGC+TGG | 0.461637 | 8.2:+79038354 | None:intergenic |
| ATTTGTGTGCGGTTTGTGTT+CGG | 0.464682 | 8.2:-79038622 | MS.gene36775:CDS |
| ACACTTTGCATCTTCAACTT+CGG | 0.466067 | 8.2:+79038716 | None:intergenic |
| CGTGTTCGTAACAAGTTTGA+AGG | 0.467318 | 8.2:-79038648 | MS.gene36775:CDS |
| TGTCGGAGACCGTTCTTTGA+AGG | 0.474473 | 8.2:+79038796 | None:intergenic |
| TTAAGTTTAACAAATATGGT+AGG | 0.490124 | 8.2:-79038521 | MS.gene36775:CDS |
| AGGGACAAGAAAGGAGGAGG+AGG | 0.494514 | 8.2:-79038405 | MS.gene36775:CDS |
| TGTTGTCAAGTTCATTCTCA+AGG | 0.519029 | 8.2:-79038869 | MS.gene36775:CDS |
| TAAGTTTAACAAATATGGTA+GGG | 0.538103 | 8.2:-79038520 | MS.gene36775:CDS |
| GGAGGCGGTGAAAGAAGAGT+TGG | 0.546775 | 8.2:-79038601 | MS.gene36775:CDS |
| GTGCGGTTTGTGTTCGGAGG+CGG | 0.552330 | 8.2:-79038616 | MS.gene36775:CDS |
| GACCGTTCTTTGAAGGTGGT+CGG | 0.559329 | 8.2:+79038803 | None:intergenic |
| ATCATGGTTATGGCACCTCA+TGG | 0.562684 | 8.2:-79038900 | MS.gene36775:CDS |
| CCAAGGGACAAGAAAGGAGG+AGG | 0.566147 | 8.2:-79038408 | MS.gene36775:CDS |
| CGGAGACCGTTCTTTGAAGG+TGG | 0.567308 | 8.2:+79038799 | None:intergenic |
| GGGCTAAGTCAATTAGTCCA+AGG | 0.570684 | 8.2:-79038425 | MS.gene36775:CDS |
| ATTAGTCCAAGGGACAAGAA+AGG | 0.576487 | 8.2:-79038414 | MS.gene36775:CDS |
| ACGAACACGATCTATGTACT+CGG | 0.578833 | 8.2:+79038662 | None:intergenic |
| ACAAGAAAGGAGGAGGAGGA+GGG | 0.591395 | 8.2:-79038401 | MS.gene36775:CDS |
| GTGTTCGTAACAAGTTTGAA+GGG | 0.597314 | 8.2:-79038647 | MS.gene36775:CDS |
| GGCTAAGTCAATTAGTCCAA+GGG | 0.598516 | 8.2:-79038424 | MS.gene36775:CDS |
| TTCCGACCACCTTCAAAGAA+CGG | 0.607623 | 8.2:-79038805 | MS.gene36775:CDS |
| CATCAGCTCGAAGGAAATGT+CGG | 0.626398 | 8.2:+79038779 | None:intergenic |
| TCTTCAACTTCGGATATCGA+TGG | 0.633325 | 8.2:+79038726 | None:intergenic |
| TGTGTGCGGTTTGTGTTCGG+AGG | 0.638466 | 8.2:-79038619 | MS.gene36775:CDS |
| CGAACACGATCTATGTACTC+GGG | 0.649900 | 8.2:+79038663 | None:intergenic |
| GATGCAAAGTGTGAGTGTTG+TGG | 0.665594 | 8.2:-79038705 | MS.gene36775:CDS |
| AGTCCAAGGGACAAGAAAGG+AGG | 0.666513 | 8.2:-79038411 | MS.gene36775:CDS |
| TTTGAAGGGAAATTTGTGTG+CGG | 0.673715 | 8.2:-79038633 | MS.gene36775:CDS |
| GACAACAAACATTCTCCATG+AGG | 0.716500 | 8.2:+79038885 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAGTTTAACAAATATGGTA+GGG | - | chr8.2:79038709-79038728 | MS.gene36775:CDS | 20.0% |
| !! | TGTGTTAAGTTTAACAAATA+TGG | - | chr8.2:79038704-79038723 | MS.gene36775:CDS | 20.0% |
| !! | TTAAGTTTAACAAATATGGT+AGG | - | chr8.2:79038708-79038727 | MS.gene36775:CDS | 20.0% |
| ! | AATGTTGAAAAAGAGTAACA+TGG | - | chr8.2:79038769-79038788 | MS.gene36775:CDS | 25.0% |
| AAAGAAGAGTTGGAGAAAAA+TGG | - | chr8.2:79038638-79038657 | MS.gene36775:CDS | 30.0% | |
| TAACATGGATGGGAAAATTA+GGG | - | chr8.2:79038784-79038803 | MS.gene36775:CDS | 30.0% | |
| TGAAAAAGAGTAACATGGAT+GGG | - | chr8.2:79038774-79038793 | MS.gene36775:CDS | 30.0% | |
| TTGAAAAAGAGTAACATGGA+TGG | - | chr8.2:79038773-79038792 | MS.gene36775:CDS | 30.0% | |
| AAGAGTTGGAGAAAAATGGT+GGG | - | chr8.2:79038642-79038661 | MS.gene36775:CDS | 35.0% | |
| ACACTTTGCATCTTCAACTT+CGG | + | chr8.2:79038516-79038535 | None:intergenic | 35.0% | |
| GTAACATGGATGGGAAAATT+AGG | - | chr8.2:79038783-79038802 | MS.gene36775:CDS | 35.0% | |
| GTGTTCGTAACAAGTTTGAA+GGG | - | chr8.2:79038582-79038601 | MS.gene36775:CDS | 35.0% | |
| TGTTGTCAAGTTCATTCTCA+AGG | - | chr8.2:79038360-79038379 | MS.gene36775:CDS | 35.0% | |
| TTTGAAGGGAAATTTGTGTG+CGG | - | chr8.2:79038596-79038615 | MS.gene36775:CDS | 35.0% | |
| !! | GAAAGCTTTGTTGGTTTGTT+TGG | + | chr8.2:79038401-79038420 | None:intergenic | 35.0% |
| ACGAACACGATCTATGTACT+CGG | + | chr8.2:79038570-79038589 | None:intergenic | 40.0% | |
| ATTAGTCCAAGGGACAAGAA+AGG | - | chr8.2:79038815-79038834 | MS.gene36775:CDS | 40.0% | |
| ATTTGTGTGCGGTTTGTGTT+CGG | - | chr8.2:79038607-79038626 | MS.gene36775:CDS | 40.0% | |
| CGTGTTCGTAACAAGTTTGA+AGG | - | chr8.2:79038581-79038600 | MS.gene36775:CDS | 40.0% | |
| GAAGAGTTGGAGAAAAATGG+TGG | - | chr8.2:79038641-79038660 | MS.gene36775:CDS | 40.0% | |
| GACAACAAACATTCTCCATG+AGG | + | chr8.2:79038347-79038366 | None:intergenic | 40.0% | |
| GCTTGTGCTTGAAACAAAAC+TGG | + | chr8.2:79038740-79038759 | None:intergenic | 40.0% | |
| GGCTAAGTCAATTAGTCCAA+GGG | - | chr8.2:79038805-79038824 | MS.gene36775:CDS | 40.0% | |
| TCTTCAACTTCGGATATCGA+TGG | + | chr8.2:79038506-79038525 | None:intergenic | 40.0% | |
| TTGATCTCTCTTGTAAGTGC+TGG | + | chr8.2:79038878-79038897 | None:intergenic | 40.0% | |
| ! | TCGGAAGTTGAAAGCTTTGT+TGG | + | chr8.2:79038410-79038429 | None:intergenic | 40.0% |
| CATCAGCTCGAAGGAAATGT+CGG | + | chr8.2:79038453-79038472 | None:intergenic | 45.0% | |
| CGAACACGATCTATGTACTC+GGG | + | chr8.2:79038569-79038588 | None:intergenic | 45.0% | |
| GGGCTAAGTCAATTAGTCCA+AGG | - | chr8.2:79038804-79038823 | MS.gene36775:CDS | 45.0% | |
| TTCCGACCACCTTCAAAGAA+CGG | - | chr8.2:79038424-79038443 | MS.gene36775:CDS | 45.0% | |
| ! | GATGCAAAGTGTGAGTGTTG+TGG | - | chr8.2:79038524-79038543 | MS.gene36775:CDS | 45.0% |
| ! | TTCTTTTGCCATCAGCTCGA+AGG | + | chr8.2:79038462-79038481 | None:intergenic | 45.0% |
| ACAAGAAAGGAGGAGGAGGA+GGG | - | chr8.2:79038828-79038847 | MS.gene36775:CDS | 50.0% | |
| AGTCCAAGGGACAAGAAAGG+AGG | - | chr8.2:79038818-79038837 | MS.gene36775:CDS | 50.0% | |
| CGACATTTCCTTCGAGCTGA+TGG | - | chr8.2:79038451-79038470 | MS.gene36775:CDS | 50.0% | |
| TGTCGGAGACCGTTCTTTGA+AGG | + | chr8.2:79038436-79038455 | None:intergenic | 50.0% | |
| ! | GACCGTTCTTTGAAGGTGGT+CGG | + | chr8.2:79038429-79038448 | None:intergenic | 50.0% |
| AGGGACAAGAAAGGAGGAGG+AGG | - | chr8.2:79038824-79038843 | MS.gene36775:CDS | 55.0% | |
| CCAAGGGACAAGAAAGGAGG+AGG | - | chr8.2:79038821-79038840 | MS.gene36775:CDS | 55.0% | |
| CCTCCTCCTTTCTTGTCCCT+TGG | + | chr8.2:79038824-79038843 | None:intergenic | 55.0% | |
| GACAAGAAAGGAGGAGGAGG+AGG | - | chr8.2:79038827-79038846 | MS.gene36775:CDS | 55.0% | |
| GGAGGCGGTGAAAGAAGAGT+TGG | - | chr8.2:79038628-79038647 | MS.gene36775:CDS | 55.0% | |
| TGTGTGCGGTTTGTGTTCGG+AGG | - | chr8.2:79038610-79038629 | MS.gene36775:CDS | 55.0% | |
| ! | CGGAGACCGTTCTTTGAAGG+TGG | + | chr8.2:79038433-79038452 | None:intergenic | 55.0% |
| GTGCGGTTTGTGTTCGGAGG+CGG | - | chr8.2:79038613-79038632 | MS.gene36775:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 79038332 | 79038919 | 79038332 | ID=MS.gene36775 |
| chr8.2 | mRNA | 79038332 | 79038919 | 79038332 | ID=MS.gene36775.t1;Parent=MS.gene36775 |
| chr8.2 | exon | 79038332 | 79038919 | 79038332 | ID=MS.gene36775.t1.exon1;Parent=MS.gene36775.t1 |
| chr8.2 | CDS | 79038332 | 79038919 | 79038332 | ID=cds.MS.gene36775.t1;Parent=MS.gene36775.t1 |
| Gene Sequence |
| Protein sequence |
