Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41132.t1 | XP_003606719.1 | 87.6 | 688 | 7 | 1 | 65 | 674 | 117 | 804 | 0.00E+00 | 1193.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41132.t1 | Q43715 | 82.3 | 688 | 44 | 1 | 65 | 674 | 122 | 809 | 0.0e+00 | 1126.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41132.t1 | G7JCF5 | 87.6 | 688 | 7 | 1 | 65 | 674 | 117 | 804 | 0.0e+00 | 1193.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049297 | MS.gene41132 | 0.838412 | 2.92E-57 | -1.69E-46 |
| MS.gene049322 | MS.gene41132 | 0.816707 | 4.81E-52 | -1.69E-46 |
| MS.gene049382 | MS.gene41132 | 0.820399 | 7.00E-53 | -1.69E-46 |
| MS.gene049440 | MS.gene41132 | 0.800727 | 1.26E-48 | -1.69E-46 |
| MS.gene049824 | MS.gene41132 | 0.81016 | 1.32E-50 | -1.69E-46 |
| MS.gene050114 | MS.gene41132 | 0.806424 | 8.28E-50 | -1.69E-46 |
| MS.gene050226 | MS.gene41132 | 0.80332 | 3.69E-49 | -1.69E-46 |
| MS.gene050227 | MS.gene41132 | 0.803001 | 4.29E-49 | -1.69E-46 |
| MS.gene050228 | MS.gene41132 | 0.800715 | 1.27E-48 | -1.69E-46 |
| MS.gene050277 | MS.gene41132 | 0.842165 | 3.05E-58 | -1.69E-46 |
| MS.gene050838 | MS.gene41132 | 0.808671 | 2.76E-50 | -1.69E-46 |
| MS.gene051063 | MS.gene41132 | 0.825889 | 3.66E-54 | -1.69E-46 |
| MS.gene051662 | MS.gene41132 | 0.820002 | 8.63E-53 | -1.69E-46 |
| MS.gene051746 | MS.gene41132 | 0.825315 | 5.01E-54 | -1.69E-46 |
| MS.gene051748 | MS.gene41132 | 0.826463 | 2.68E-54 | -1.69E-46 |
| MS.gene051850 | MS.gene41132 | 0.811364 | 7.27E-51 | -1.69E-46 |
| MS.gene051903 | MS.gene41132 | 0.814473 | 1.51E-51 | -1.69E-46 |
| MS.gene052202 | MS.gene41132 | 0.811308 | 7.47E-51 | -1.69E-46 |
| MS.gene052254 | MS.gene41132 | 0.832418 | 9.56E-56 | -1.69E-46 |
| MS.gene052256 | MS.gene41132 | 0.850144 | 2.05E-60 | -1.69E-46 |
| MS.gene052286 | MS.gene41132 | 0.805736 | 1.16E-49 | -1.69E-46 |
| MS.gene052302 | MS.gene41132 | 0.835244 | 1.88E-56 | -1.69E-46 |
| MS.gene052376 | MS.gene41132 | 0.831088 | 2.04E-55 | -1.69E-46 |
| MS.gene052600 | MS.gene41132 | 0.822216 | 2.67E-53 | -1.69E-46 |
| MS.gene052665 | MS.gene41132 | 0.815462 | 9.13E-52 | -1.69E-46 |
| MS.gene052856 | MS.gene41132 | 0.863088 | 3.19E-64 | -1.69E-46 |
| MS.gene052858 | MS.gene41132 | 0.840214 | 9.95E-58 | -1.69E-46 |
| MS.gene052860 | MS.gene41132 | 0.832708 | 8.10E-56 | -1.69E-46 |
| MS.gene052947 | MS.gene41132 | 0.800264 | 1.56E-48 | -1.69E-46 |
| MS.gene053132 | MS.gene41132 | 0.806012 | 1.01E-49 | -1.69E-46 |
| MS.gene053437 | MS.gene41132 | 0.822552 | 2.23E-53 | -1.69E-46 |
| MS.gene054942 | MS.gene41132 | 0.845331 | 4.34E-59 | -1.69E-46 |
| MS.gene055174 | MS.gene41132 | 0.806868 | 6.67E-50 | -1.69E-46 |
| MS.gene055424 | MS.gene41132 | 0.815171 | 1.06E-51 | -1.69E-46 |
| MS.gene056403 | MS.gene41132 | 0.821284 | 4.38E-53 | -1.69E-46 |
| MS.gene056432 | MS.gene41132 | 0.845905 | 3.03E-59 | -1.69E-46 |
| MS.gene056552 | MS.gene41132 | 0.814491 | 1.50E-51 | -1.69E-46 |
| MS.gene056605 | MS.gene41132 | 0.816954 | 4.24E-52 | -1.69E-46 |
| MS.gene056631 | MS.gene41132 | 0.81442 | 1.55E-51 | -1.69E-46 |
| MS.gene056760 | MS.gene41132 | 0.807206 | 5.66E-50 | -1.69E-46 |
| MS.gene057057 | MS.gene41132 | 0.813047 | 3.12E-51 | -1.69E-46 |
| MS.gene057094 | MS.gene41132 | 0.839774 | 1.30E-57 | -1.69E-46 |
| MS.gene057234 | MS.gene41132 | 0.803124 | 4.05E-49 | -1.69E-46 |
| MS.gene058182 | MS.gene41132 | 0.822634 | 2.13E-53 | -1.69E-46 |
| MS.gene058238 | MS.gene41132 | 0.813372 | 2.65E-51 | -1.69E-46 |
| MS.gene058833 | MS.gene41132 | 0.815559 | 8.69E-52 | -1.69E-46 |
| MS.gene058851 | MS.gene41132 | 0.809142 | 2.19E-50 | -1.69E-46 |
| MS.gene058906 | MS.gene41132 | 0.831936 | 1.26E-55 | -1.69E-46 |
| MS.gene058982 | MS.gene41132 | 0.802197 | 6.29E-49 | -1.69E-46 |
| MS.gene059171 | MS.gene41132 | 0.802583 | 5.24E-49 | -1.69E-46 |
| MS.gene05978 | MS.gene41132 | 0.810269 | 1.25E-50 | -1.69E-46 |
| MS.gene06001 | MS.gene41132 | 0.889158 | 3.11E-73 | -1.69E-46 |
| MS.gene060165 | MS.gene41132 | 0.802037 | 6.78E-49 | -1.69E-46 |
| MS.gene060619 | MS.gene41132 | 0.847632 | 1.02E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41132.t1 | MTR_4g064780 | 99.289 | 422 | 3 | 0 | 253 | 674 | 383 | 804 | 0.0 | 869 |
| MS.gene41132.t1 | MTR_4g064780 | 96.842 | 190 | 6 | 0 | 65 | 254 | 117 | 306 | 4.16e-125 | 390 |
| MS.gene41132.t1 | MTR_2g097700 | 85.986 | 421 | 59 | 0 | 254 | 674 | 361 | 781 | 0.0 | 780 |
| MS.gene41132.t1 | MTR_2g097700 | 59.474 | 190 | 77 | 0 | 65 | 254 | 94 | 283 | 5.81e-73 | 251 |
| MS.gene41132.t1 | MTR_4g064780 | 98.842 | 259 | 3 | 0 | 253 | 511 | 383 | 641 | 0.0 | 529 |
| MS.gene41132.t1 | MTR_4g064780 | 96.842 | 190 | 6 | 0 | 65 | 254 | 117 | 306 | 2.53e-127 | 391 |
| MS.gene41132.t1 | MTR_5g013940 | 78.000 | 100 | 22 | 0 | 509 | 608 | 20 | 119 | 9.48e-52 | 177 |
| MS.gene41132.t1 | MTR_2g098110 | 25.428 | 409 | 256 | 14 | 257 | 658 | 291 | 657 | 7.04e-19 | 91.7 |
| MS.gene41132.t1 | MTR_1g070230 | 32.487 | 197 | 110 | 7 | 469 | 658 | 157 | 337 | 1.74e-14 | 75.9 |
| MS.gene41132.t1 | MTR_2g087590 | 26.820 | 261 | 151 | 7 | 420 | 658 | 102 | 344 | 4.29e-14 | 74.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41132.t1 | AT3G46740 | 84.834 | 422 | 64 | 0 | 253 | 674 | 397 | 818 | 0.0 | 771 |
| MS.gene41132.t1 | AT3G46740 | 68.947 | 190 | 59 | 0 | 65 | 254 | 131 | 320 | 8.90e-86 | 286 |
| MS.gene41132.t1 | AT4G09080 | 61.928 | 415 | 137 | 7 | 262 | 674 | 1 | 396 | 2.43e-175 | 505 |
| MS.gene41132.t1 | AT4G09080 | 61.928 | 415 | 137 | 7 | 262 | 674 | 12 | 407 | 2.65e-175 | 506 |
| MS.gene41132.t1 | AT4G09080 | 70.154 | 325 | 94 | 3 | 352 | 674 | 2 | 325 | 1.27e-163 | 473 |
| MS.gene41132.t1 | AT1G35860 | 50.620 | 403 | 103 | 5 | 250 | 649 | 90 | 399 | 3.31e-122 | 369 |
| MS.gene41132.t1 | AT5G19620 | 25.527 | 427 | 257 | 16 | 257 | 671 | 350 | 727 | 1.50e-19 | 94.0 |
| MS.gene41132.t1 | AT3G44160 | 33.480 | 227 | 119 | 9 | 420 | 630 | 102 | 312 | 6.85e-17 | 83.6 |
Find 199 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATTACCTCGAGGATGTTTC+TGG | 0.196346 | 4.1:-36099707 | None:intergenic |
| TGAGGTTTATAAAGAGATTA+AGG | 0.210672 | 4.1:+36096820 | MS.gene41132:CDS |
| GAATCCCAATCTTGTGATTT+TGG | 0.214936 | 4.1:-36096335 | None:intergenic |
| CGTCAGAGTAAATTCACTTA+TGG | 0.227562 | 4.1:+36098041 | MS.gene41132:CDS |
| TACTTCTAGGCCTTGTCTTT+TGG | 0.238382 | 4.1:+36096793 | MS.gene41132:CDS |
| TAAAATTACCTCAGTGATAT+TGG | 0.265879 | 4.1:-36097919 | None:intergenic |
| TATGAGTGAAAAGGAGAAAA+TGG | 0.277825 | 4.1:+36096727 | MS.gene41132:CDS |
| GGATGATCTTGCATTTAAAA+TGG | 0.280908 | 4.1:+36097740 | MS.gene41132:intron |
| GGAGTATGCACATCCGTATT+TGG | 0.292124 | 4.1:+36097761 | MS.gene41132:CDS |
| GGTTCTGGCCTTGCGTGTTC+TGG | 0.310325 | 4.1:-36092368 | None:intergenic |
| AAAATTGTACTTGGTGGTTC+TGG | 0.310760 | 4.1:-36092383 | None:intergenic |
| GAAACTGAGTCAAAGTTAAT+TGG | 0.330853 | 4.1:-36099519 | None:intergenic |
| GCGAGCATACCGTTTGGGTT+AGG | 0.332381 | 4.1:-36092435 | None:intergenic |
| CGGAATCTTATGATGGAATC+AGG | 0.334858 | 4.1:+36092818 | MS.gene41132:CDS |
| ATGGGTCAGGGGTCCTCTTA+TGG | 0.335391 | 4.1:+36099960 | MS.gene41132:CDS |
| CCAGTATTCACTGGTGGGCC+GGG | 0.337871 | 4.1:+36097849 | MS.gene41132:CDS |
| CTCAAAATCTTTCTCCAAAT+CGG | 0.339518 | 4.1:-36100058 | None:intergenic |
| CGAAACACCAAGACGAAAAT+CGG | 0.346482 | 4.1:+36096446 | MS.gene41132:CDS |
| TCGTCGGCGAACGCGGTTGC+TGG | 0.348068 | 4.1:-36096311 | None:intergenic |
| TCCAGTATTCACTGGTGGGC+CGG | 0.351628 | 4.1:+36097848 | MS.gene41132:CDS |
| CCCGGCCCACCAGTGAATAC+TGG | 0.359227 | 4.1:-36097849 | None:intergenic |
| GCAGAACATGGCACCGATCT+AGG | 0.359815 | 4.1:+36099891 | MS.gene41132:CDS |
| TACACGTGTGTACCTTTAAT+AGG | 0.361255 | 4.1:-36099861 | None:intergenic |
| CGAGCATACCGTTTGGGTTA+GGG | 0.371757 | 4.1:-36092434 | None:intergenic |
| TTGACAAAACAGCTTCTTCC+GGG | 0.376101 | 4.1:+36097184 | MS.gene41132:intron |
| TGGAAGCAGGAACATGTTCC+AGG | 0.393764 | 4.1:+36098259 | MS.gene41132:CDS |
| GGATTGAATAGATCTCTTAC+TGG | 0.396182 | 4.1:+36097563 | MS.gene41132:CDS |
| ACTGAGTGGAGTATTGTTCC+TGG | 0.398835 | 4.1:+36097373 | MS.gene41132:CDS |
| TTTGACAAAACAGCTTCTTC+CGG | 0.401319 | 4.1:+36097183 | MS.gene41132:intron |
| CTGATTCCATCATAAGATTC+CGG | 0.404234 | 4.1:-36092817 | None:intergenic |
| CCAGTACCACTGAGGGTTGT+TGG | 0.404945 | 4.1:-36098164 | None:intergenic |
| GACAAGAGAGGGAGTATAAG+AGG | 0.407066 | 4.1:+36096762 | MS.gene41132:CDS |
| TTGAATCCGCTAAGTTTGTT+TGG | 0.407816 | 4.1:-36096389 | None:intergenic |
| TGGATGAAGTGCCCTCCATA+TGG | 0.410464 | 4.1:+36097874 | MS.gene41132:CDS |
| GTTGTAACCCCTTACAGAAT+AGG | 0.411697 | 4.1:-36099665 | None:intergenic |
| AGCGTTGACGATTTCGTTCG+CGG | 0.412504 | 4.1:+36096619 | MS.gene41132:CDS |
| CTTCCCTCATTCTTCTCATC+AGG | 0.414103 | 4.1:-36097295 | None:intergenic |
| CAGTATTCACTGGTGGGCCG+GGG | 0.414933 | 4.1:+36097850 | MS.gene41132:CDS |
| TTGTAACCCCTTACAGAATA+GGG | 0.418439 | 4.1:-36099664 | None:intergenic |
| AGAGGAGGATTGATACTTCT+AGG | 0.418613 | 4.1:+36096780 | MS.gene41132:CDS |
| AATTGGTGACGGTTAAAGAA+TGG | 0.420841 | 4.1:-36099502 | None:intergenic |
| TCAGTCGAAACCGGTTGAAA+TGG | 0.421087 | 4.1:+36096697 | MS.gene41132:CDS |
| AATGTTTGAGAAGGTTGATA+TGG | 0.422128 | 4.1:+36096568 | MS.gene41132:CDS |
| CTTCCTCCACAGACTTCAAC+TGG | 0.424159 | 4.1:-36099543 | None:intergenic |
| ACGAACCAAAATCACAAGAT+TGG | 0.424406 | 4.1:+36096330 | MS.gene41132:CDS |
| GAGGTACGTATACCTATTAA+AGG | 0.424670 | 4.1:+36099849 | MS.gene41132:CDS |
| GGTTACAACATGGGTGAGAT+TGG | 0.425561 | 4.1:+36099679 | MS.gene41132:CDS |
| TGAATACTGGACTCAGCTTT+CGG | 0.425621 | 4.1:-36097836 | None:intergenic |
| CTTGTCCTCCATGGTCACTA+TGG | 0.425624 | 4.1:+36099592 | MS.gene41132:CDS |
| ATGTATCAATGTAGGGTTGA+TGG | 0.426742 | 4.1:+36096673 | MS.gene41132:CDS |
| ACCATTGGAACAGATATGAT+TGG | 0.428498 | 4.1:-36098099 | None:intergenic |
| CAGGGGTCCTCTTATGGTGC+TGG | 0.428552 | 4.1:+36099966 | MS.gene41132:CDS |
| CGCTAATGTTCCATCAGCAT+TGG | 0.435932 | 4.1:-36096600 | None:intergenic |
| CAACACTCAGTTGTACCGTT+TGG | 0.436053 | 4.1:+36092408 | MS.gene41132:CDS |
| ATTGGTGACGGTTAAAGAAT+GGG | 0.436136 | 4.1:-36099501 | None:intergenic |
| TGTTTGCGAGCATACCGTTT+GGG | 0.439289 | 4.1:-36092440 | None:intergenic |
| GAAATCCGACAGAAGTCTAT+AGG | 0.441636 | 4.1:+36099934 | MS.gene41132:CDS |
| CTTCATACCAGCACCATAAG+AGG | 0.442191 | 4.1:-36099973 | None:intergenic |
| GAGGTTTATAAAGAGATTAA+GGG | 0.444103 | 4.1:+36096821 | MS.gene41132:CDS |
| AGTCTATAGGAGAATGGGTC+AGG | 0.444715 | 4.1:+36099947 | MS.gene41132:CDS |
| GGTAGAACGGTTATGTTAGC+TGG | 0.445300 | 4.1:-36096368 | None:intergenic |
| AGGTTTAGATGTATCAATGT+AGG | 0.447359 | 4.1:+36096665 | MS.gene41132:CDS |
| CTGCAGGCTTCGCTTCAGCC+AGG | 0.449356 | 4.1:+36097506 | MS.gene41132:intron |
| ACCAGCACGGTCAACCCATA+TGG | 0.451429 | 4.1:-36097889 | None:intergenic |
| TTAATCTCTTTATAAACCTC+AGG | 0.452420 | 4.1:-36096818 | None:intergenic |
| AGCAGCTTACACGGAGAGTT+CGG | 0.456701 | 4.1:-36097806 | None:intergenic |
| GTATGTGGGAGAAAGCTGAT+AGG | 0.458850 | 4.1:+36096645 | MS.gene41132:CDS |
| TAAAGAGTTGGAGCAGAAAT+CGG | 0.460513 | 4.1:+36097339 | MS.gene41132:CDS |
| TGAGATTAAGCTTAAAGAGT+TGG | 0.462130 | 4.1:+36097327 | MS.gene41132:CDS |
| TTTCGTTCGCGGAGAGTATG+TGG | 0.462541 | 4.1:+36096630 | MS.gene41132:CDS |
| CCAACAACCCTCAGTGGTAC+TGG | 0.463318 | 4.1:+36098164 | MS.gene41132:CDS |
| TATGGTGCTGGTATGAAGCT+TGG | 0.464083 | 4.1:+36099978 | MS.gene41132:CDS |
| ATATCAGGATCCATTTCAAC+CGG | 0.464958 | 4.1:-36096707 | None:intergenic |
| AAGGGGATGTTGAGTAAGCA+AGG | 0.465253 | 4.1:+36096839 | MS.gene41132:CDS |
| TCTGCACTAATTCCTCCATT+AGG | 0.471078 | 4.1:-36098134 | None:intergenic |
| GGTCAAAGAGTATTGCCTAA+TGG | 0.474904 | 4.1:+36098119 | MS.gene41132:CDS |
| AGGCAATACTCTTTGACCAT+TGG | 0.475159 | 4.1:-36098114 | None:intergenic |
| CGAACCAAAATCACAAGATT+GGG | 0.476541 | 4.1:+36096331 | MS.gene41132:CDS |
| GTTGATCATAACTCAGGAAC+AGG | 0.476580 | 4.1:+36100023 | MS.gene41132:CDS |
| TTTGTGAACGGAGCTATAGT+TGG | 0.480304 | 4.1:+36098239 | MS.gene41132:CDS |
| GGATGTTGAGTAAGCAAGGG+AGG | 0.483099 | 4.1:+36096843 | MS.gene41132:CDS |
| CCGCGGATTCACTTCAATGT+TGG | 0.485045 | 4.1:-36097272 | None:intergenic |
| ATTCACTTATGGATTAGTAA+TGG | 0.488117 | 4.1:+36098052 | MS.gene41132:CDS |
| GACAGAAGTCTATAGGAGAA+TGG | 0.488499 | 4.1:+36099941 | MS.gene41132:CDS |
| TGGAGTATTGTTCCTGGGCG+AGG | 0.491841 | 4.1:+36097379 | MS.gene41132:CDS |
| TTCGCCGCAAATACCAAAGT+TGG | 0.492832 | 4.1:-36097409 | None:intergenic |
| GTATTGTTCCTGGGCGAGGA+GGG | 0.493554 | 4.1:+36097383 | MS.gene41132:CDS |
| GCTAAGTTTGTTTGGTAGAA+CGG | 0.495514 | 4.1:-36096381 | None:intergenic |
| GCGAGGAGGGCGTCCAACTT+TGG | 0.496872 | 4.1:+36097396 | MS.gene41132:CDS |
| GTTTCTCCAGTTGAAGTCTG+TGG | 0.496953 | 4.1:+36099537 | MS.gene41132:CDS |
| GGATCCTGATATGAGTGAAA+AGG | 0.500579 | 4.1:+36096718 | MS.gene41132:CDS |
| ACAGAAGTCTATAGGAGAAT+GGG | 0.503850 | 4.1:+36099942 | MS.gene41132:CDS |
| CTTCCTACAGCTGGCAGCTG+AGG | 0.508063 | 4.1:+36099830 | MS.gene41132:intron |
| AAGCTGGGAAACAAGCTTTG+AGG | 0.509682 | 4.1:+36097224 | MS.gene41132:CDS |
| TTCTGTAAGGGGTTACAACA+TGG | 0.513783 | 4.1:+36099669 | MS.gene41132:CDS |
| TCATTCTTCTCATCAGGCCG+CGG | 0.514700 | 4.1:-36097289 | None:intergenic |
| TCATTTACACCATCCAAATA+CGG | 0.518980 | 4.1:-36097774 | None:intergenic |
| GTTGCATTTCACATCTCCCA+TGG | 0.519662 | 4.1:-36092884 | None:intergenic |
| GGACTATTACATTTGAACAT+AGG | 0.519926 | 4.1:+36097531 | MS.gene41132:CDS |
| TTTGAGAAGGTTGATATGGA+AGG | 0.520196 | 4.1:+36096572 | MS.gene41132:CDS |
| TCAAAACTGCAGGTAGATCA+AGG | 0.521033 | 4.1:+36099457 | MS.gene41132:intron |
| GTTGATGGGTCAGTCGAAAC+CGG | 0.521835 | 4.1:+36096688 | MS.gene41132:CDS |
| AGTATTCACTGGTGGGCCGG+GGG | 0.524172 | 4.1:+36097851 | MS.gene41132:CDS |
| CTGCAGGTAGATCAAGGCCT+TGG | 0.525854 | 4.1:+36099463 | MS.gene41132:intron |
| GGTTTAGATGTATCAATGTA+GGG | 0.527431 | 4.1:+36096666 | MS.gene41132:CDS |
| AAACCTCCGGAATCTTATGA+TGG | 0.527623 | 4.1:+36092811 | MS.gene41132:CDS |
| TCCATATGGGTTGACCGTGC+TGG | 0.529325 | 4.1:+36097888 | MS.gene41132:CDS |
| TGTATCAATGTAGGGTTGAT+GGG | 0.529350 | 4.1:+36096674 | MS.gene41132:CDS |
| ACCAATCATATCTGTTCCAA+TGG | 0.531948 | 4.1:+36098098 | MS.gene41132:CDS |
| AGAGTAAAAGCATCATAACT+TGG | 0.532470 | 4.1:-36099634 | None:intergenic |
| TCGTCTTGGTGTTTCGATCG+AGG | 0.534927 | 4.1:-36096439 | None:intergenic |
| TGGTACTGGTATCGATAAGA+TGG | 0.535826 | 4.1:+36098178 | MS.gene41132:CDS |
| AAGAGAGGGAGTATAAGAGG+AGG | 0.538246 | 4.1:+36096765 | MS.gene41132:CDS |
| GGCTGTTGAAGCAGCTTACA+CGG | 0.541865 | 4.1:-36097815 | None:intergenic |
| AATGGAGGAATTAGTGCAGA+TGG | 0.543155 | 4.1:+36098137 | MS.gene41132:CDS |
| GTTCTACCAAACAAACTTAG+CGG | 0.546093 | 4.1:+36096383 | MS.gene41132:CDS |
| GTTGGACGCCCTCCTCGCCC+AGG | 0.555798 | 4.1:-36097391 | None:intergenic |
| TTCATTCACCAGAACACGCA+AGG | 0.557375 | 4.1:+36092360 | MS.gene41132:CDS |
| CCACCAGTGCTTGTCCTCCA+TGG | 0.557742 | 4.1:+36099583 | MS.gene41132:CDS |
| AGGACAAGCACTGGTGGTGG+TGG | 0.558995 | 4.1:-36099577 | None:intergenic |
| CAGGCTTCGCTTCAGCCAGG+AGG | 0.559209 | 4.1:+36097509 | MS.gene41132:intron |
| TTTGGTGTCCCTAACCCAAA+CGG | 0.559433 | 4.1:+36092426 | MS.gene41132:CDS |
| GATGTTGAGTAAGCAAGGGA+GGG | 0.559985 | 4.1:+36096844 | MS.gene41132:CDS |
| GAAGGGAAAACCAATGCTGA+TGG | 0.561077 | 4.1:+36096590 | MS.gene41132:CDS |
| TTGAGAAGGTTGATATGGAA+GGG | 0.561158 | 4.1:+36096573 | MS.gene41132:CDS |
| ATGGTCCCTCAAGAAACCTC+CGG | 0.561643 | 4.1:+36092798 | MS.gene41132:intron |
| ACACGGAGAGTTCGGTTGCG+TGG | 0.562344 | 4.1:-36097798 | None:intergenic |
| GGATGAAGTGCCCTCCATAT+GGG | 0.563784 | 4.1:+36097875 | MS.gene41132:CDS |
| CTCGATTATCATCAAGCCAT+GGG | 0.565963 | 4.1:+36092868 | MS.gene41132:CDS |
| GTGTATGCCTTTGCAGAACA+TGG | 0.566174 | 4.1:+36099879 | MS.gene41132:CDS |
| TAGCAACTTCTTGAATCCTC+AGG | 0.567074 | 4.1:+36097598 | MS.gene41132:CDS |
| TTTGGGTTAGGGACACCAAA+CGG | 0.567135 | 4.1:-36092423 | None:intergenic |
| TTATGATGGAATCAGGGACT+CGG | 0.569234 | 4.1:+36092825 | MS.gene41132:CDS |
| GGTCACTATGGTGGTTGTGT+AGG | 0.572314 | 4.1:+36099604 | MS.gene41132:CDS |
| AAGCTGAGTCCAGTATTCAC+TGG | 0.572634 | 4.1:+36097840 | MS.gene41132:CDS |
| AGGCTTCGCTTCAGCCAGGA+GGG | 0.573416 | 4.1:+36097510 | MS.gene41132:intron |
| GAGGGCACTTCATCCACCCC+CGG | 0.574911 | 4.1:-36097867 | None:intergenic |
| AAGTCTGTGGAGGAAGGTGC+TGG | 0.575143 | 4.1:+36099550 | MS.gene41132:CDS |
| CAATGTTGGAAAACAAAGCA+AGG | 0.575395 | 4.1:-36097258 | None:intergenic |
| GAGTCAAAGTTAATTGGTGA+CGG | 0.579143 | 4.1:-36099513 | None:intergenic |
| TTCGTTCGCGGAGAGTATGT+GGG | 0.579595 | 4.1:+36096631 | MS.gene41132:CDS |
| CAGTTGAAGTCTGTGGAGGA+AGG | 0.582370 | 4.1:+36099544 | MS.gene41132:CDS |
| CTGAGTGGAGTATTGTTCCT+GGG | 0.582646 | 4.1:+36097374 | MS.gene41132:CDS |
| GTAGATCAAGGCCTTGGTGT+TGG | 0.582725 | 4.1:+36099469 | MS.gene41132:CDS |
| AGTATTGTTCCTGGGCGAGG+AGG | 0.583721 | 4.1:+36097382 | MS.gene41132:CDS |
| AGGGGATGTTGAGTAAGCAA+GGG | 0.584583 | 4.1:+36096840 | MS.gene41132:CDS |
| GTCTATAGGAGAATGGGTCA+GGG | 0.584875 | 4.1:+36099948 | MS.gene41132:CDS |
| GGAAGTTCAAAAGATGTCAA+AGG | 0.585343 | 4.1:+36099912 | MS.gene41132:CDS |
| GCGGCCTGATGAGAAGAATG+AGG | 0.586532 | 4.1:+36097291 | MS.gene41132:CDS |
| TCAAATGTAATAGTCCCTCC+TGG | 0.588321 | 4.1:-36097524 | None:intergenic |
| ATTCTCCTATAGACTTCTGT+CGG | 0.588523 | 4.1:-36099939 | None:intergenic |
| ACGGAGCTATAGTTGGAAGC+AGG | 0.591154 | 4.1:+36098246 | MS.gene41132:CDS |
| CTAGAAAGTTTAGCTACATG+TGG | 0.591287 | 4.1:+36096545 | MS.gene41132:CDS |
| TATGCACATCCGTATTTGGA+TGG | 0.591352 | 4.1:+36097765 | MS.gene41132:CDS |
| CGGCCTGATGAGAAGAATGA+GGG | 0.591730 | 4.1:+36097292 | MS.gene41132:CDS |
| AGGTTTATAAAGAGATTAAG+GGG | 0.594005 | 4.1:+36096822 | MS.gene41132:CDS |
| CACAACCACCATAGTGACCA+TGG | 0.599148 | 4.1:-36099600 | None:intergenic |
| ATCGGTGCCATGTTCTGCAA+AGG | 0.600282 | 4.1:-36099886 | None:intergenic |
| GCTCGATTATCATCAAGCCA+TGG | 0.602193 | 4.1:+36092867 | MS.gene41132:CDS |
| CTGAGTCCAGTATTCACTGG+TGG | 0.604435 | 4.1:+36097843 | MS.gene41132:CDS |
| GGAATCTTATGATGGAATCA+GGG | 0.605731 | 4.1:+36092819 | MS.gene41132:CDS |
| AGCACGGTCAACCCATATGG+AGG | 0.608832 | 4.1:-36097886 | None:intergenic |
| TATGCGGTTGATCATAACTC+AGG | 0.608919 | 4.1:+36100017 | MS.gene41132:CDS |
| TGACCATGGAGGACAAGCAC+TGG | 0.609873 | 4.1:-36099586 | None:intergenic |
| ACCTCAGGAGCCAAAAGACA+AGG | 0.610574 | 4.1:-36096803 | None:intergenic |
| CAGGGACTCGGATGAAGACG+TGG | 0.614274 | 4.1:+36092837 | MS.gene41132:CDS |
| TTTACAAGCTAATATTACAA+GGG | 0.614814 | 4.1:+36098205 | MS.gene41132:CDS |
| GTCCTCCATGGTCACTATGG+TGG | 0.615702 | 4.1:+36099595 | MS.gene41132:CDS |
| CACACGCGCTCAGCTTCAGA+AGG | 0.620020 | 4.1:+36096520 | MS.gene41132:CDS |
| TGAGTCCAGTATTCACTGGT+GGG | 0.621283 | 4.1:+36097844 | MS.gene41132:CDS |
| CAAAGAGTATTGCCTAATGG+AGG | 0.624544 | 4.1:+36098122 | MS.gene41132:CDS |
| TCTGTAAGGGGTTACAACAT+GGG | 0.625335 | 4.1:+36099670 | MS.gene41132:CDS |
| GCGCGTGTGTATACACCACC+TGG | 0.629270 | 4.1:-36096506 | None:intergenic |
| GTACCACTGAGGGTTGTTGG+AGG | 0.632265 | 4.1:-36098161 | None:intergenic |
| TCTCCAGTTGAAGTCTGTGG+AGG | 0.634494 | 4.1:+36099540 | MS.gene41132:CDS |
| TGGAGGACAAGCACTGGTGG+TGG | 0.638082 | 4.1:-36099580 | None:intergenic |
| ATCGATACCAGTACCACTGA+GGG | 0.640712 | 4.1:-36098171 | None:intergenic |
| TTTGAACATAGGAATCTGCA+AGG | 0.642514 | 4.1:+36097542 | MS.gene41132:CDS |
| CGGCTGAAGTTAGTACTGAG+TGG | 0.644179 | 4.1:+36097359 | MS.gene41132:CDS |
| AATGTTGGAAAACAAAGCAA+GGG | 0.649560 | 4.1:-36097257 | None:intergenic |
| CCAACATTGAAGTGAATCCG+CGG | 0.651264 | 4.1:+36097272 | MS.gene41132:CDS |
| GACATGGTATCCCTAAAACC+AGG | 0.657656 | 4.1:+36096488 | MS.gene41132:CDS |
| TCTATAGGAGAATGGGTCAG+GGG | 0.660220 | 4.1:+36099949 | MS.gene41132:CDS |
| GGAAATTGCTGCCAACACCA+AGG | 0.665668 | 4.1:-36099480 | None:intergenic |
| GAAGCTTGGTTTAGTGAGAG+CGG | 0.666822 | 4.1:+36099992 | MS.gene41132:CDS |
| GCACGGTCAACCCATATGGA+GGG | 0.670151 | 4.1:-36097885 | None:intergenic |
| TACATGTGGAATGTTTGAGA+AGG | 0.671038 | 4.1:+36096559 | MS.gene41132:CDS |
| CCATGGAGGACAAGCACTGG+TGG | 0.671793 | 4.1:-36099583 | None:intergenic |
| ATTCCATCATAAGATTCCGG+AGG | 0.674790 | 4.1:-36092814 | None:intergenic |
| TTTAGTGAGAGCGGAATATG+CGG | 0.680967 | 4.1:+36100001 | MS.gene41132:CDS |
| TCAATGTTAAAAGTATGACC+CGG | 0.683764 | 4.1:-36097202 | None:intergenic |
| GATAACACAAGGTTTGTGAA+CGG | 0.685190 | 4.1:+36098227 | MS.gene41132:CDS |
| GGACCTCCAACAACCCTCAG+TGG | 0.685641 | 4.1:+36098158 | MS.gene41132:CDS |
| ATATTACAAGGGATAACACA+AGG | 0.704360 | 4.1:+36098216 | MS.gene41132:CDS |
| TATCGATACCAGTACCACTG+AGG | 0.732661 | 4.1:-36098172 | None:intergenic |
| ATGGTATCCCTAAAACCAGG+TGG | 0.758207 | 4.1:+36096491 | MS.gene41132:CDS |
| AGCAGCCAGAAACATCCTCG+AGG | 0.763048 | 4.1:+36099702 | MS.gene41132:CDS |
| TGTAAAAGCCAATATCACTG+AGG | 0.767187 | 4.1:+36097911 | MS.gene41132:CDS |
| AACCACCATAGTGACCATGG+AGG | 0.809946 | 4.1:-36099597 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 36092346 | 36100079 | 36092346 | ID=MS.gene41132 |
| chr4.1 | mRNA | 36092346 | 36100079 | 36092346 | ID=MS.gene41132.t1;Parent=MS.gene41132 |
| chr4.1 | exon | 36092346 | 36092447 | 36092346 | ID=MS.gene41132.t1.exon1;Parent=MS.gene41132.t1 |
| chr4.1 | CDS | 36092346 | 36092447 | 36092346 | ID=cds.MS.gene41132.t1;Parent=MS.gene41132.t1 |
| chr4.1 | exon | 36092811 | 36092894 | 36092811 | ID=MS.gene41132.t1.exon2;Parent=MS.gene41132.t1 |
| chr4.1 | CDS | 36092811 | 36092894 | 36092811 | ID=cds.MS.gene41132.t1;Parent=MS.gene41132.t1 |
| chr4.1 | exon | 36094345 | 36094352 | 36094345 | ID=MS.gene41132.t1.exon3;Parent=MS.gene41132.t1 |
| chr4.1 | CDS | 36094345 | 36094352 | 36094345 | ID=cds.MS.gene41132.t1;Parent=MS.gene41132.t1 |
| chr4.1 | exon | 36096301 | 36096865 | 36096301 | ID=MS.gene41132.t1.exon4;Parent=MS.gene41132.t1 |
| chr4.1 | CDS | 36096301 | 36096865 | 36096301 | ID=cds.MS.gene41132.t1;Parent=MS.gene41132.t1 |
| chr4.1 | exon | 36097196 | 36097417 | 36097196 | ID=MS.gene41132.t1.exon5;Parent=MS.gene41132.t1 |
| chr4.1 | CDS | 36097196 | 36097417 | 36097196 | ID=cds.MS.gene41132.t1;Parent=MS.gene41132.t1 |
| chr4.1 | exon | 36097512 | 36097619 | 36097512 | ID=MS.gene41132.t1.exon6;Parent=MS.gene41132.t1 |
| chr4.1 | CDS | 36097512 | 36097619 | 36097512 | ID=cds.MS.gene41132.t1;Parent=MS.gene41132.t1 |
| chr4.1 | exon | 36097741 | 36097932 | 36097741 | ID=MS.gene41132.t1.exon7;Parent=MS.gene41132.t1 |
| chr4.1 | CDS | 36097741 | 36097932 | 36097741 | ID=cds.MS.gene41132.t1;Parent=MS.gene41132.t1 |
| chr4.1 | exon | 36098032 | 36098280 | 36098032 | ID=MS.gene41132.t1.exon8;Parent=MS.gene41132.t1 |
| chr4.1 | CDS | 36098032 | 36098280 | 36098032 | ID=cds.MS.gene41132.t1;Parent=MS.gene41132.t1 |
| chr4.1 | exon | 36099469 | 36099723 | 36099469 | ID=MS.gene41132.t1.exon9;Parent=MS.gene41132.t1 |
| chr4.1 | CDS | 36099469 | 36099723 | 36099469 | ID=cds.MS.gene41132.t1;Parent=MS.gene41132.t1 |
| chr4.1 | exon | 36099840 | 36100079 | 36099840 | ID=MS.gene41132.t1.exon10;Parent=MS.gene41132.t1 |
| chr4.1 | CDS | 36099840 | 36100079 | 36099840 | ID=cds.MS.gene41132.t1;Parent=MS.gene41132.t1 |
| Gene Sequence |
| Protein sequence |
