Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41631.t1 | XP_003602735.1 | 99.7 | 311 | 1 | 0 | 1 | 311 | 1 | 311 | 8.80E-177 | 629.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41631.t1 | Q9M2E2 | 41.8 | 297 | 139 | 7 | 37 | 310 | 9 | 294 | 1.3e-51 | 204.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41631.t1 | G7J2S1 | 99.7 | 311 | 1 | 0 | 1 | 311 | 1 | 311 | 6.4e-177 | 629.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene57562 | MS.gene41631 | PPI |
| MS.gene41631 | MS.gene58348 | PPI |
| MS.gene41631 | MS.gene39579 | PPI |
| MS.gene41631 | MS.gene009163 | PPI |
| MS.gene57559 | MS.gene41631 | PPI |
| MS.gene70985 | MS.gene41631 | PPI |
| MS.gene58269 | MS.gene41631 | PPI |
| MS.gene47525 | MS.gene41631 | PPI |
| MS.gene58272 | MS.gene41631 | PPI |
| MS.gene019535 | MS.gene41631 | PPI |
| MS.gene008719 | MS.gene41631 | PPI |
| MS.gene41631 | MS.gene019537 | PPI |
| MS.gene41631 | MS.gene58344 | PPI |
| MS.gene019536 | MS.gene41631 | PPI |
| MS.gene019540 | MS.gene41631 | PPI |
| MS.gene26577 | MS.gene41631 | PPI |
| MS.gene41631 | MS.gene038616 | PPI |
| MS.gene58267 | MS.gene41631 | PPI |
| MS.gene58347 | MS.gene41631 | PPI |
| MS.gene41631 | MS.gene57563 | PPI |
| MS.gene58271 | MS.gene41631 | PPI |
| MS.gene019532 | MS.gene41631 | PPI |
| MS.gene58346 | MS.gene41631 | PPI |
| MS.gene41631 | MS.gene57564 | PPI |
| MS.gene57558 | MS.gene41631 | PPI |
| MS.gene019531 | MS.gene41631 | PPI |
| MS.gene58270 | MS.gene41631 | PPI |
| MS.gene019530 | MS.gene41631 | PPI |
| MS.gene57560 | MS.gene41631 | PPI |
| MS.gene58268 | MS.gene41631 | PPI |
| MS.gene41631 | MS.gene58343 | PPI |
| MS.gene019527 | MS.gene41631 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41631.t1 | MTR_3g098470 | 99.678 | 311 | 1 | 0 | 1 | 311 | 1 | 311 | 0.0 | 642 |
| MS.gene41631.t1 | MTR_8g096060 | 80.511 | 313 | 56 | 2 | 1 | 311 | 1 | 310 | 0.0 | 521 |
| MS.gene41631.t1 | MTR_1g031180 | 77.926 | 299 | 66 | 0 | 13 | 311 | 18 | 316 | 2.53e-180 | 500 |
| MS.gene41631.t1 | MTR_4g087460 | 41.695 | 295 | 154 | 8 | 29 | 311 | 16 | 304 | 3.61e-73 | 228 |
| MS.gene41631.t1 | MTR_8g032010 | 41.077 | 297 | 138 | 5 | 33 | 303 | 5 | 290 | 2.69e-69 | 218 |
| MS.gene41631.t1 | MTR_8g032000 | 41.017 | 295 | 137 | 5 | 37 | 305 | 9 | 292 | 8.94e-69 | 216 |
| MS.gene41631.t1 | MTR_8g031960 | 40.667 | 300 | 140 | 6 | 36 | 309 | 9 | 296 | 1.31e-67 | 213 |
| MS.gene41631.t1 | MTR_7g076660 | 41.522 | 289 | 144 | 6 | 37 | 311 | 9 | 286 | 1.33e-67 | 213 |
| MS.gene41631.t1 | MTR_7g076660 | 41.522 | 289 | 144 | 6 | 37 | 311 | 10 | 287 | 1.61e-67 | 213 |
| MS.gene41631.t1 | MTR_8g031980 | 39.604 | 303 | 145 | 6 | 34 | 310 | 6 | 296 | 2.97e-67 | 212 |
| MS.gene41631.t1 | MTR_8g031990 | 39.344 | 305 | 148 | 6 | 33 | 311 | 5 | 298 | 7.27e-67 | 211 |
| MS.gene41631.t1 | MTR_7g076655 | 41.522 | 289 | 144 | 6 | 37 | 311 | 9 | 286 | 6.38e-66 | 208 |
| MS.gene41631.t1 | MTR_8g032020 | 40.397 | 302 | 142 | 6 | 37 | 311 | 10 | 300 | 7.79e-66 | 209 |
| MS.gene41631.t1 | MTR_5g062280 | 40.132 | 304 | 146 | 6 | 32 | 311 | 4 | 295 | 1.95e-64 | 205 |
| MS.gene41631.t1 | MTR_8g031910 | 41.724 | 290 | 143 | 6 | 32 | 306 | 4 | 282 | 1.04e-62 | 200 |
| MS.gene41631.t1 | MTR_3g079320 | 38.079 | 302 | 148 | 5 | 31 | 303 | 7 | 298 | 1.13e-61 | 198 |
| MS.gene41631.t1 | MTR_8g031940 | 39.456 | 294 | 140 | 6 | 37 | 303 | 9 | 291 | 4.59e-61 | 196 |
| MS.gene41631.t1 | MTR_8g031630 | 38.538 | 301 | 147 | 9 | 37 | 311 | 9 | 297 | 5.44e-61 | 196 |
| MS.gene41631.t1 | MTR_8g031900 | 37.162 | 296 | 154 | 5 | 37 | 311 | 9 | 293 | 6.69e-61 | 196 |
| MS.gene41631.t1 | MTR_8g031610 | 38.667 | 300 | 147 | 8 | 37 | 311 | 7 | 294 | 8.82e-61 | 196 |
| MS.gene41631.t1 | MTR_8g031650 | 38.333 | 300 | 147 | 9 | 38 | 311 | 104 | 391 | 4.69e-59 | 194 |
| MS.gene41631.t1 | MTR_8g031650 | 39.669 | 121 | 62 | 4 | 177 | 292 | 2 | 116 | 5.73e-16 | 78.2 |
| MS.gene41631.t1 | MTR_7g076660 | 40.074 | 272 | 138 | 6 | 54 | 311 | 2 | 262 | 7.27e-59 | 189 |
| MS.gene41631.t1 | MTR_8g031600 | 38.614 | 303 | 149 | 7 | 35 | 311 | 7 | 298 | 5.38e-58 | 189 |
| MS.gene41631.t1 | MTR_8g031650 | 38.333 | 300 | 147 | 9 | 38 | 311 | 270 | 557 | 1.58e-57 | 194 |
| MS.gene41631.t1 | MTR_8g031650 | 39.298 | 285 | 133 | 9 | 37 | 292 | 9 | 282 | 6.31e-55 | 187 |
| MS.gene41631.t1 | MTR_7g076665 | 37.919 | 298 | 151 | 5 | 37 | 311 | 9 | 295 | 2.42e-56 | 184 |
| MS.gene41631.t1 | MTR_7g106800 | 37.234 | 282 | 128 | 7 | 37 | 311 | 11 | 250 | 2.11e-49 | 166 |
| MS.gene41631.t1 | MTR_8g031850 | 33.453 | 278 | 151 | 4 | 37 | 311 | 9 | 255 | 9.37e-47 | 158 |
| MS.gene41631.t1 | MTR_5g083430 | 37.245 | 196 | 111 | 4 | 116 | 311 | 1 | 184 | 3.29e-37 | 131 |
| MS.gene41631.t1 | MTR_8g032030 | 42.012 | 169 | 70 | 3 | 34 | 175 | 6 | 173 | 4.53e-35 | 127 |
| MS.gene41631.t1 | MTR_4g105310 | 29.252 | 294 | 150 | 11 | 37 | 308 | 51 | 308 | 1.71e-16 | 79.3 |
| MS.gene41631.t1 | MTR_8g099995 | 27.907 | 301 | 147 | 14 | 37 | 308 | 38 | 297 | 2.62e-14 | 72.8 |
| MS.gene41631.t1 | MTR_8g098415 | 28.669 | 293 | 146 | 13 | 37 | 305 | 32 | 285 | 2.13e-13 | 70.1 |
| MS.gene41631.t1 | MTR_8g098415 | 28.669 | 293 | 146 | 13 | 37 | 305 | 32 | 285 | 2.73e-13 | 69.7 |
| MS.gene41631.t1 | MTR_4g105310 | 29.457 | 258 | 133 | 9 | 37 | 287 | 51 | 266 | 5.36e-13 | 68.2 |
| MS.gene41631.t1 | MTR_3g068070 | 34.028 | 144 | 92 | 3 | 29 | 170 | 12 | 154 | 1.36e-12 | 67.0 |
| MS.gene41631.t1 | MTR_1g100305 | 28.425 | 292 | 148 | 13 | 37 | 305 | 32 | 285 | 1.65e-11 | 64.3 |
| MS.gene41631.t1 | MTR_8g442750 | 29.932 | 147 | 96 | 2 | 33 | 172 | 15 | 161 | 5.59e-11 | 62.8 |
| MS.gene41631.t1 | MTR_7g071680 | 31.034 | 174 | 110 | 4 | 10 | 177 | 6 | 175 | 6.53e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41631.t1 | AT5G51030 | 69.968 | 313 | 92 | 1 | 1 | 311 | 2 | 314 | 4.85e-173 | 482 |
| MS.gene41631.t1 | AT5G61830 | 67.089 | 316 | 99 | 3 | 1 | 311 | 1 | 316 | 8.45e-158 | 443 |
| MS.gene41631.t1 | AT5G61830 | 67.089 | 316 | 99 | 3 | 1 | 311 | 1 | 316 | 8.45e-158 | 443 |
| MS.gene41631.t1 | AT3G59710 | 42.756 | 283 | 151 | 5 | 23 | 303 | 7 | 280 | 2.60e-77 | 238 |
| MS.gene41631.t1 | AT3G59710 | 41.554 | 296 | 161 | 6 | 11 | 303 | 23 | 309 | 4.75e-76 | 236 |
| MS.gene41631.t1 | AT3G59710 | 42.199 | 282 | 152 | 5 | 24 | 303 | 18 | 290 | 1.14e-74 | 231 |
| MS.gene41631.t1 | AT3G61220 | 41.414 | 297 | 140 | 6 | 37 | 310 | 9 | 294 | 9.61e-67 | 211 |
| MS.gene41631.t1 | AT3G61220 | 41.414 | 297 | 140 | 6 | 37 | 310 | 16 | 301 | 1.41e-66 | 211 |
| MS.gene41631.t1 | AT3G61220 | 41.414 | 297 | 140 | 6 | 37 | 310 | 40 | 325 | 3.11e-66 | 211 |
| MS.gene41631.t1 | AT2G24190 | 40.741 | 297 | 142 | 6 | 37 | 310 | 9 | 294 | 1.94e-65 | 207 |
| MS.gene41631.t1 | AT2G24190 | 40.741 | 297 | 142 | 6 | 37 | 310 | 14 | 299 | 2.50e-65 | 207 |
| MS.gene41631.t1 | AT1G01800 | 39.535 | 301 | 146 | 7 | 36 | 311 | 6 | 295 | 1.56e-64 | 205 |
| MS.gene41631.t1 | AT1G01800 | 39.891 | 183 | 99 | 3 | 129 | 311 | 89 | 260 | 2.07e-37 | 134 |
| MS.gene41631.t1 | AT4G23420 | 30.375 | 293 | 142 | 11 | 37 | 305 | 49 | 303 | 3.92e-16 | 78.2 |
| MS.gene41631.t1 | AT4G23420 | 30.375 | 293 | 142 | 11 | 37 | 305 | 32 | 286 | 8.24e-16 | 77.0 |
| MS.gene41631.t1 | AT4G23420 | 30.375 | 293 | 142 | 11 | 37 | 305 | 32 | 286 | 8.24e-16 | 77.0 |
| MS.gene41631.t1 | AT5G50130 | 30.180 | 222 | 126 | 7 | 25 | 221 | 27 | 244 | 1.87e-15 | 75.1 |
| MS.gene41631.t1 | AT5G50130 | 26.984 | 315 | 159 | 11 | 25 | 310 | 27 | 299 | 1.92e-15 | 76.3 |
| MS.gene41631.t1 | AT5G50130 | 30.317 | 221 | 126 | 7 | 25 | 221 | 27 | 243 | 2.83e-15 | 74.7 |
| MS.gene41631.t1 | AT2G37540 | 27.211 | 294 | 149 | 11 | 37 | 305 | 36 | 289 | 2.98e-15 | 75.5 |
| MS.gene41631.t1 | AT5G50130 | 30.180 | 222 | 126 | 7 | 25 | 221 | 27 | 244 | 3.11e-15 | 75.5 |
| MS.gene41631.t1 | AT5G50130 | 30.317 | 221 | 126 | 7 | 25 | 221 | 27 | 243 | 3.85e-15 | 75.1 |
| MS.gene41631.t1 | AT5G50130 | 27.575 | 301 | 147 | 11 | 39 | 310 | 8 | 266 | 1.61e-13 | 70.5 |
| MS.gene41631.t1 | AT5G50130 | 27.667 | 300 | 147 | 11 | 39 | 310 | 8 | 265 | 2.14e-13 | 70.1 |
| MS.gene41631.t1 | AT5G50130 | 31.250 | 208 | 114 | 7 | 39 | 221 | 8 | 211 | 2.91e-13 | 69.3 |
| MS.gene41631.t1 | AT5G50130 | 31.401 | 207 | 114 | 7 | 39 | 221 | 8 | 210 | 3.69e-13 | 68.9 |
| MS.gene41631.t1 | AT4G23420 | 31.064 | 235 | 133 | 10 | 37 | 248 | 32 | 260 | 1.72e-12 | 67.0 |
| MS.gene41631.t1 | AT4G23420 | 31.064 | 235 | 131 | 9 | 37 | 248 | 32 | 258 | 6.22e-12 | 65.1 |
| MS.gene41631.t1 | AT1G64590 | 27.703 | 296 | 154 | 12 | 37 | 308 | 37 | 296 | 1.98e-11 | 64.3 |
| MS.gene41631.t1 | AT4G20760 | 28.834 | 163 | 100 | 4 | 33 | 179 | 42 | 204 | 6.66e-11 | 62.4 |
| MS.gene41631.t1 | AT4G20760 | 28.834 | 163 | 100 | 4 | 33 | 179 | 62 | 224 | 6.99e-11 | 62.8 |
| MS.gene41631.t1 | AT4G23430 | 27.682 | 289 | 145 | 11 | 37 | 305 | 32 | 276 | 9.26e-11 | 62.4 |
Find 72 sgRNAs with CRISPR-Local
Find 129 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTATTGAAACAAATTATTA+TGG | 0.185787 | 3.4:+88352651 | MS.gene41631:CDS |
| TCACGTTTGGGTCGTCTAAA+TGG | 0.278823 | 3.4:+88352750 | MS.gene41631:CDS |
| GAAACTATTGCCGTGGTTAC+TGG | 0.305445 | 3.4:+88352219 | MS.gene41631:CDS |
| GAAGATGGAAGTTGGAAAAC+AGG | 0.313685 | 3.4:+88353492 | MS.gene41631:CDS |
| TGTTAATGTGAGTTCACGTT+TGG | 0.336548 | 3.4:+88352737 | MS.gene41631:CDS |
| ATATGGTGGTCTAGATATTC+TGG | 0.337491 | 3.4:+88352443 | MS.gene41631:CDS |
| GTTCCTGATCAAGAGATTAC+AGG | 0.348866 | 3.4:+88353729 | MS.gene41631:CDS |
| AACCTCTGGTGATCAGAATA+AGG | 0.350644 | 3.4:-88351880 | None:intergenic |
| GTTGAACAAGGGGCTGATAC+TGG | 0.370186 | 3.4:+88353690 | MS.gene41631:CDS |
| ACTGGTGGAAACCGAGGAAT+TGG | 0.387985 | 3.4:+88352237 | MS.gene41631:CDS |
| AAGGGGCTGATACTGGAGTT+TGG | 0.401013 | 3.4:+88353697 | MS.gene41631:CDS |
| TAATACTTACACAAGGTATA+TGG | 0.404526 | 3.4:+88353563 | MS.gene41631:CDS |
| AATAAGGAATGGTTCTCAAA+AGG | 0.409576 | 3.4:-88351864 | None:intergenic |
| GTATTGAAACAAATTATTAT+GGG | 0.416441 | 3.4:+88352652 | MS.gene41631:CDS |
| TACATTAATTGTTACTGTCC+TGG | 0.416925 | 3.4:+88353624 | MS.gene41631:CDS |
| AATAGAAAATGAGGAGTTGA+GGG | 0.424375 | 3.4:+88353395 | MS.gene41631:CDS |
| TGGGTGAAAACTGCTCTAAC+AGG | 0.426724 | 3.4:+88353648 | MS.gene41631:CDS |
| GGTCTTGCAAGAAGGTGGTT+TGG | 0.437244 | 3.4:+88352347 | MS.gene41631:CDS |
| AACCACGGCAATAGTTTCCT+TGG | 0.438839 | 3.4:-88352214 | None:intergenic |
| CATAGCTTCAATCATTCTCT+TGG | 0.438910 | 3.4:-88352676 | None:intergenic |
| TCAAGACAACTTGCCGATCA+TGG | 0.440043 | 3.4:+88352270 | MS.gene41631:CDS |
| TTTCTACAACAAGTAGAAGA+TGG | 0.445780 | 3.4:+88353477 | MS.gene41631:CDS |
| GTTAATGTGAGTTCACGTTT+GGG | 0.446917 | 3.4:+88352738 | MS.gene41631:CDS |
| TATTTATAGGTAAATAATGC+TGG | 0.447071 | 3.4:+88352579 | MS.gene41631:intron |
| TTCCCTGTAATCTCTTGATC+AGG | 0.452132 | 3.4:-88353732 | None:intergenic |
| ATAAGGAATGGTTCTCAAAA+GGG | 0.455119 | 3.4:-88351863 | None:intergenic |
| TTGATGAAGCCATCTGCTGC+TGG | 0.468286 | 3.4:+88352705 | MS.gene41631:CDS |
| GAATAGAAAATGAGGAGTTG+AGG | 0.479987 | 3.4:+88353394 | MS.gene41631:CDS |
| GAGTGGTTGAAAGAAGAATA+TGG | 0.483507 | 3.4:+88352426 | MS.gene41631:CDS |
| TGGCAAAGAAGCTCTCTGAT+AGG | 0.485202 | 3.4:+88353583 | MS.gene41631:CDS |
| AACAAGTAGAAGATGGAAGT+TGG | 0.488568 | 3.4:+88353484 | MS.gene41631:CDS |
| AAATCTCAAAACCAATTCCT+CGG | 0.493628 | 3.4:-88352248 | None:intergenic |
| ACTGCTCTAACAGGTTATGC+AGG | 0.511056 | 3.4:+88353657 | MS.gene41631:CDS |
| GAATAGTCAGTAAATGTTTG+AGG | 0.522157 | 3.4:-88353522 | None:intergenic |
| TATATAAAGGTGGTGGTCCA+AGG | 0.527053 | 3.4:+88352197 | MS.gene41631:intron |
| TAATTGTTACTGTCCTGGTT+GGG | 0.532693 | 3.4:+88353629 | MS.gene41631:CDS |
| TTAATTGTTACTGTCCTGGT+TGG | 0.540425 | 3.4:+88353628 | MS.gene41631:CDS |
| GTTACAGAGAATAGAAAATG+AGG | 0.541317 | 3.4:+88353386 | MS.gene41631:intron |
| TAATCTCTTGATCAGGAACA+AGG | 0.543992 | 3.4:-88353725 | None:intergenic |
| GCAGCAGATGGCTTCATCAA+AGG | 0.554615 | 3.4:-88352702 | None:intergenic |
| CACAACAGTAACTCCATGAT+CGG | 0.568398 | 3.4:-88352283 | None:intergenic |
| ATCAAGGTCTTGCAAGAAGG+TGG | 0.574693 | 3.4:+88352342 | MS.gene41631:CDS |
| TCAATCAAGGTCTTGCAAGA+AGG | 0.574992 | 3.4:+88352339 | MS.gene41631:CDS |
| TGCTGGTGTTAATTCCAACA+TGG | 0.582462 | 3.4:+88352596 | MS.gene41631:CDS |
| TTCCTGATCAAGAGATTACA+GGG | 0.585333 | 3.4:+88353730 | MS.gene41631:CDS |
| TTCCTTATTCTGATCACCAG+AGG | 0.589818 | 3.4:+88351878 | MS.gene41631:CDS |
| TGTCGGTGTTGAATCAATCA+AGG | 0.590040 | 3.4:+88352326 | MS.gene41631:CDS |
| GGAAGTGTTACAGTTGAACA+AGG | 0.606460 | 3.4:+88353678 | MS.gene41631:CDS |
| GATGGAAGTTGGAAAACAGG+AGG | 0.608453 | 3.4:+88353495 | MS.gene41631:CDS |
| ATGGAAGTTGGAAAACAGGA+GGG | 0.612855 | 3.4:+88353496 | MS.gene41631:CDS |
| GCTGGTGTTAATTCCAACAT+GGG | 0.616735 | 3.4:+88352597 | MS.gene41631:CDS |
| ACTATTGCCGTGGTTACTGG+TGG | 0.620364 | 3.4:+88352222 | MS.gene41631:CDS |
| ATGAAGCCATCTGCTGCTGG+TGG | 0.625719 | 3.4:+88352708 | MS.gene41631:CDS |
| ATATATCAAGTTGATGACAA+TGG | 0.627304 | 3.4:-88352374 | None:intergenic |
| ATACGACCACCAGCAGCAGA+TGG | 0.629788 | 3.4:-88352714 | None:intergenic |
| CTGGTGATCAGAATAAGGAA+TGG | 0.631585 | 3.4:-88351875 | None:intergenic |
| AAGTGTTACAGTTGAACAAG+GGG | 0.633975 | 3.4:+88353680 | MS.gene41631:CDS |
| GTCCAAGGAAACTATTGCCG+TGG | 0.637747 | 3.4:+88352212 | MS.gene41631:CDS |
| TGATGTGGAATCTCTTTCAG+AGG | 0.642229 | 3.4:+88353428 | MS.gene41631:CDS |
| GAGGGAGAAACTGAGTGATG+TGG | 0.647729 | 3.4:+88353413 | MS.gene41631:CDS |
| AAGCTCTCTGATAGGCCAGA+GGG | 0.648245 | 3.4:+88353591 | MS.gene41631:CDS |
| TCGGTTTCCACCAGTAACCA+CGG | 0.650998 | 3.4:-88352229 | None:intergenic |
| TGAGTTATCAGATCCCATGT+TGG | 0.651992 | 3.4:-88352610 | None:intergenic |
| ACATCAAGAGATGCTAGTGT+CGG | 0.652928 | 3.4:+88352309 | MS.gene41631:CDS |
| GTGGTTACTGGTGGAAACCG+AGG | 0.666460 | 3.4:+88352231 | MS.gene41631:CDS |
| GAAGTGTTACAGTTGAACAA+GGG | 0.666525 | 3.4:+88353679 | MS.gene41631:CDS |
| GAAGCTCTCTGATAGGCCAG+AGG | 0.680052 | 3.4:+88353590 | MS.gene41631:CDS |
| GAAGTTGGAAAACAGGAGGG+TGG | 0.681062 | 3.4:+88353499 | MS.gene41631:CDS |
| AATCTCTTGATCAGGAACAA+GGG | 0.687916 | 3.4:-88353724 | None:intergenic |
| CTGTCAATGAATTTGCTGAG+TGG | 0.698650 | 3.4:+88352409 | MS.gene41631:CDS |
| TTGCAGTTAATACTTACACA+AGG | 0.716694 | 3.4:+88353556 | MS.gene41631:CDS |
| TGGTTGAAAGAAGAATATGG+TGG | 0.790075 | 3.4:+88352429 | MS.gene41631:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTAAAATCATGATTTTAA+CGG | - | chr3.4:88352166-88352185 | None:intergenic | 10.0% |
| !! | ATTGTTAAATACTATGATAT+GGG | - | chr3.4:88353194-88353213 | None:intergenic | 15.0% |
| !! | GTATTGAAACAAATTATTAT+GGG | + | chr3.4:88352652-88352671 | MS.gene41631:CDS | 15.0% |
| !! | TGTATTGAAACAAATTATTA+TGG | + | chr3.4:88352651-88352670 | MS.gene41631:CDS | 15.0% |
| !! | TTGAACAATAAAAGAATTAT+AGG | - | chr3.4:88351928-88351947 | None:intergenic | 15.0% |
| !!! | AATGACATTTTATAGTTTTA+GGG | + | chr3.4:88353239-88353258 | MS.gene41631:intron | 15.0% |
| !!! | GCTATAATTTTGTTATTTAT+AGG | + | chr3.4:88352566-88352585 | MS.gene41631:intron | 15.0% |
| !!! | TATAATTATCAATTGTTTCT+TGG | + | chr3.4:88352544-88352563 | MS.gene41631:intron | 15.0% |
| !! | CATATCATAGTATTTAACAA+TGG | + | chr3.4:88353193-88353212 | MS.gene41631:intron | 20.0% |
| !! | CATTGTTAAATACTATGATA+TGG | - | chr3.4:88353195-88353214 | None:intergenic | 20.0% |
| !! | TATGTTACCTTTCTATATAA+AGG | + | chr3.4:88352184-88352203 | MS.gene41631:intron | 20.0% |
| !! | TATTTAACAATGGAAAGTAA+CGG | + | chr3.4:88353203-88353222 | MS.gene41631:intron | 20.0% |
| !! | TATTTATAGGTAAATAATGC+TGG | + | chr3.4:88352579-88352598 | MS.gene41631:intron | 20.0% |
| !!! | GAATGACATTTTATAGTTTT+AGG | + | chr3.4:88353238-88353257 | MS.gene41631:intron | 20.0% |
| !!! | TTAATTTGACTAAGAAACTT+AGG | - | chr3.4:88353103-88353122 | None:intergenic | 20.0% |
| !!! | TTCGACTTAATTTTGTTTAT+AGG | + | chr3.4:88352503-88352522 | MS.gene41631:intron | 20.0% |
| ! | ATATATTTCCCAATTAGCAA+GGG | + | chr3.4:88353262-88353281 | MS.gene41631:intron | 25.0% |
| ! | ATGAGATACCTAAATTTCAT+AGG | - | chr3.4:88353014-88353033 | None:intergenic | 25.0% |
| ! | ATGTTCATATGATAATCAGA+AGG | - | chr3.4:88351976-88351995 | None:intergenic | 25.0% |
| ! | CATCATTGAAATTGTATGTT+TGG | - | chr3.4:88353297-88353316 | None:intergenic | 25.0% |
| ! | GACTGGATTGAAATATATAT+GGG | - | chr3.4:88352066-88352085 | None:intergenic | 25.0% |
| ! | TAATACTTACACAAGGTATA+TGG | + | chr3.4:88353563-88353582 | MS.gene41631:CDS | 25.0% |
| ! | TAATGAGCTTATATTGATGT+AGG | + | chr3.4:88353127-88353146 | MS.gene41631:intron | 25.0% |
| ! | TACATCAATATAAGCTCATT+AGG | - | chr3.4:88353128-88353147 | None:intergenic | 25.0% |
| ! | TATAAGATCAAATTAACCTC+TGG | - | chr3.4:88351897-88351916 | None:intergenic | 25.0% |
| ! | TATGAAATTTAGGTATCTCA+TGG | + | chr3.4:88353013-88353032 | MS.gene41631:intron | 25.0% |
| ! | TTAATCTGCCTATGAAATTT+AGG | + | chr3.4:88353003-88353022 | MS.gene41631:intron | 25.0% |
| !!! | AAGTCGAATTTCACATTTTT+TGG | - | chr3.4:88352491-88352510 | None:intergenic | 25.0% |
| !!! | ATATATCAAGTTGATGACAA+TGG | - | chr3.4:88352377-88352396 | None:intergenic | 25.0% |
| AAATCTCAAAACCAATTCCT+CGG | - | chr3.4:88352251-88352270 | None:intergenic | 30.0% | |
| AATAAGGAATGGTTCTCAAA+AGG | - | chr3.4:88351867-88351886 | None:intergenic | 30.0% | |
| AATAGAAAATGAGGAGTTGA+GGG | + | chr3.4:88353395-88353414 | MS.gene41631:CDS | 30.0% | |
| ATAAGGAATGGTTCTCAAAA+GGG | - | chr3.4:88351866-88351885 | None:intergenic | 30.0% | |
| GAATAGTCAGTAAATGTTTG+AGG | - | chr3.4:88353525-88353544 | None:intergenic | 30.0% | |
| GATATATTTCCCAATTAGCA+AGG | + | chr3.4:88353261-88353280 | MS.gene41631:intron | 30.0% | |
| GGACTGGATTGAAATATATA+TGG | - | chr3.4:88352067-88352086 | None:intergenic | 30.0% | |
| GTTACAGAGAATAGAAAATG+AGG | + | chr3.4:88353386-88353405 | MS.gene41631:intron | 30.0% | |
| GTTACCTTTCTATATAAAGG+TGG | + | chr3.4:88352187-88352206 | MS.gene41631:intron | 30.0% | |
| TACATTAATTGTTACTGTCC+TGG | + | chr3.4:88353624-88353643 | MS.gene41631:CDS | 30.0% | |
| TTCATAGGCAGATTAATCTA+CGG | - | chr3.4:88352999-88353018 | None:intergenic | 30.0% | |
| TTGCAGTTAATACTTACACA+AGG | + | chr3.4:88353556-88353575 | MS.gene41631:CDS | 30.0% | |
| TTTCTACAACAAGTAGAAGA+TGG | + | chr3.4:88353477-88353496 | MS.gene41631:CDS | 30.0% | |
| !! | TGGTGCTATTAATATGGTTA+AGG | - | chr3.4:88352974-88352993 | None:intergenic | 30.0% |
| AACAAGTAGAAGATGGAAGT+TGG | + | chr3.4:88353484-88353503 | MS.gene41631:CDS | 35.0% | |
| AAGTGTTACAGTTGAACAAG+GGG | + | chr3.4:88353680-88353699 | MS.gene41631:CDS | 35.0% | |
| AATACTATGATATGGGCATG+TGG | - | chr3.4:88353187-88353206 | None:intergenic | 35.0% | |
| AATCTCTTGATCAGGAACAA+GGG | - | chr3.4:88353727-88353746 | None:intergenic | 35.0% | |
| ACCACCACCTTTATATAGAA+AGG | - | chr3.4:88352194-88352213 | None:intergenic | 35.0% | |
| ACCTTTCTATATAAAGGTGG+TGG | + | chr3.4:88352190-88352209 | MS.gene41631:intron | 35.0% | |
| ATATGGTGGTCTAGATATTC+TGG | + | chr3.4:88352443-88352462 | MS.gene41631:CDS | 35.0% | |
| CAAATAGTCCCTTGCTAATT+GGG | - | chr3.4:88353273-88353292 | None:intergenic | 35.0% | |
| CATAGCTTCAATCATTCTCT+TGG | - | chr3.4:88352679-88352698 | None:intergenic | 35.0% | |
| GAATAGAAAATGAGGAGTTG+AGG | + | chr3.4:88353394-88353413 | MS.gene41631:CDS | 35.0% | |
| GAGTGGTTGAAAGAAGAATA+TGG | + | chr3.4:88352426-88352445 | MS.gene41631:CDS | 35.0% | |
| GTTAATGTGAGTTCACGTTT+GGG | + | chr3.4:88352738-88352757 | MS.gene41631:CDS | 35.0% | |
| TAATCTCTTGATCAGGAACA+AGG | - | chr3.4:88353728-88353747 | None:intergenic | 35.0% | |
| TAATTGTTACTGTCCTGGTT+GGG | + | chr3.4:88353629-88353648 | MS.gene41631:CDS | 35.0% | |
| TCAAATAGTCCCTTGCTAAT+TGG | - | chr3.4:88353274-88353293 | None:intergenic | 35.0% | |
| TCACTAGTTGCAATTGTTCA+TGG | - | chr3.4:88353060-88353079 | None:intergenic | 35.0% | |
| TGGTTGAAAGAAGAATATGG+TGG | + | chr3.4:88352429-88352448 | MS.gene41631:CDS | 35.0% | |
| TGTTAATGTGAGTTCACGTT+TGG | + | chr3.4:88352737-88352756 | MS.gene41631:CDS | 35.0% | |
| TTAACAATGGAAAGTAACGG+AGG | + | chr3.4:88353206-88353225 | MS.gene41631:intron | 35.0% | |
| TTAATTGTTACTGTCCTGGT+TGG | + | chr3.4:88353628-88353647 | MS.gene41631:CDS | 35.0% | |
| TTCCTGATCAAGAGATTACA+GGG | + | chr3.4:88353730-88353749 | MS.gene41631:CDS | 35.0% | |
| ! | ATGTAAATCTTTTCACCCTC+TGG | - | chr3.4:88353609-88353628 | None:intergenic | 35.0% |
| ! | GAAGTGTTACAGTTGAACAA+GGG | + | chr3.4:88353679-88353698 | MS.gene41631:CDS | 35.0% |
| ! | TAGGTATCTCATGGACTAAT+TGG | + | chr3.4:88353022-88353041 | MS.gene41631:intron | 35.0% |
| AACCTCTGGTGATCAGAATA+AGG | - | chr3.4:88351883-88351902 | None:intergenic | 40.0% | |
| ACATCAAGAGATGCTAGTGT+CGG | + | chr3.4:88352309-88352328 | MS.gene41631:CDS | 40.0% | |
| ATCCAATGACCACCAATGTA+CGG | + | chr3.4:88352098-88352117 | MS.gene41631:intron | 40.0% | |
| ATGGAAGTTGGAAAACAGGA+GGG | + | chr3.4:88353496-88353515 | MS.gene41631:CDS | 40.0% | |
| CACAACAGTAACTCCATGAT+CGG | - | chr3.4:88352286-88352305 | None:intergenic | 40.0% | |
| CATTCACACAATCCGTACAT+TGG | - | chr3.4:88352113-88352132 | None:intergenic | 40.0% | |
| CATTCACCCAATCAACATGT+TGG | + | chr3.4:88352015-88352034 | MS.gene41631:intron | 40.0% | |
| CTCCAACGCTAAAAGGTTAA+TGG | - | chr3.4:88352937-88352956 | None:intergenic | 40.0% | |
| CTGGTGATCAGAATAAGGAA+TGG | - | chr3.4:88351878-88351897 | None:intergenic | 40.0% | |
| GAAGATGGAAGTTGGAAAAC+AGG | + | chr3.4:88353492-88353511 | MS.gene41631:CDS | 40.0% | |
| GTTCCTGATCAAGAGATTAC+AGG | + | chr3.4:88353729-88353748 | MS.gene41631:CDS | 40.0% | |
| TCAATCAAGGTCTTGCAAGA+AGG | + | chr3.4:88352339-88352358 | MS.gene41631:CDS | 40.0% | |
| TCATAGTCTCCAACGCTAAA+AGG | - | chr3.4:88352944-88352963 | None:intergenic | 40.0% | |
| TGAGTTATCAGATCCCATGT+TGG | - | chr3.4:88352613-88352632 | None:intergenic | 40.0% | |
| TGATGTGGAATCTCTTTCAG+AGG | + | chr3.4:88353428-88353447 | MS.gene41631:CDS | 40.0% | |
| TTCCCTGTAATCTCTTGATC+AGG | - | chr3.4:88353735-88353754 | None:intergenic | 40.0% | |
| TTCCTTATTCTGATCACCAG+AGG | + | chr3.4:88351878-88351897 | MS.gene41631:CDS | 40.0% | |
| ! | CTCCATTAACCTTTTAGCGT+TGG | + | chr3.4:88352932-88352951 | MS.gene41631:intron | 40.0% |
| ! | GCTGGTGTTAATTCCAACAT+GGG | + | chr3.4:88352597-88352616 | MS.gene41631:CDS | 40.0% |
| ! | TATATAAAGGTGGTGGTCCA+AGG | + | chr3.4:88352197-88352216 | MS.gene41631:intron | 40.0% |
| ! | TGCTGGTGTTAATTCCAACA+TGG | + | chr3.4:88352596-88352615 | MS.gene41631:CDS | 40.0% |
| !! | ACGGTGTGGTGCTATTAATA+TGG | - | chr3.4:88352980-88352999 | None:intergenic | 40.0% |
| !! | CTGTCAATGAATTTGCTGAG+TGG | + | chr3.4:88352409-88352428 | MS.gene41631:CDS | 40.0% |
| !! | GGAAGTGTTACAGTTGAACA+AGG | + | chr3.4:88353678-88353697 | MS.gene41631:CDS | 40.0% |
| !! | TGTCGGTGTTGAATCAATCA+AGG | + | chr3.4:88352326-88352345 | MS.gene41631:CDS | 40.0% |
| AACCACGGCAATAGTTTCCT+TGG | - | chr3.4:88352217-88352236 | None:intergenic | 45.0% | |
| ACGACCACCAACATGTTGAT+TGG | - | chr3.4:88352025-88352044 | None:intergenic | 45.0% | |
| ACTGCTCTAACAGGTTATGC+AGG | + | chr3.4:88353657-88353676 | MS.gene41631:CDS | 45.0% | |
| AGGCAGATTAATCTACGGTG+TGG | - | chr3.4:88352994-88353013 | None:intergenic | 45.0% | |
| ATCAAGGTCTTGCAAGAAGG+TGG | + | chr3.4:88352342-88352361 | MS.gene41631:CDS | 45.0% | |
| GAAACTATTGCCGTGGTTAC+TGG | + | chr3.4:88352219-88352238 | MS.gene41631:CDS | 45.0% | |
| GATGGAAGTTGGAAAACAGG+AGG | + | chr3.4:88353495-88353514 | MS.gene41631:CDS | 45.0% | |
| GTCATTGGATTGAGATCAGG+CGG | - | chr3.4:88352088-88352107 | None:intergenic | 45.0% | |
| GTGGTCATTGGATTGAGATC+AGG | - | chr3.4:88352091-88352110 | None:intergenic | 45.0% | |
| TCAAGACAACTTGCCGATCA+TGG | + | chr3.4:88352270-88352289 | MS.gene41631:CDS | 45.0% | |
| TCACACAATCCGTACATTGG+TGG | - | chr3.4:88352110-88352129 | None:intergenic | 45.0% | |
| TCACCCAATCAACATGTTGG+TGG | + | chr3.4:88352018-88352037 | MS.gene41631:intron | 45.0% | |
| TCACGTTTGGGTCGTCTAAA+TGG | + | chr3.4:88352750-88352769 | MS.gene41631:CDS | 45.0% | |
| TGGCAAAGAAGCTCTCTGAT+AGG | + | chr3.4:88353583-88353602 | MS.gene41631:CDS | 45.0% | |
| TGGGTGAAAACTGCTCTAAC+AGG | + | chr3.4:88353648-88353667 | MS.gene41631:CDS | 45.0% | |
| ! | ATCCGTACATTGGTGGTCAT+TGG | - | chr3.4:88352103-88352122 | None:intergenic | 45.0% |
| ! | CGACCACCAACATGTTGATT+GGG | - | chr3.4:88352024-88352043 | None:intergenic | 45.0% |
| ! | GTACGGATTGTGTGAATGTG+TGG | + | chr3.4:88352115-88352134 | MS.gene41631:intron | 45.0% |
| !! | ATCAACATGTTGGTGGTCGT+CGG | + | chr3.4:88352025-88352044 | MS.gene41631:intron | 45.0% |
| AAGCTCTCTGATAGGCCAGA+GGG | + | chr3.4:88353591-88353610 | MS.gene41631:CDS | 50.0% | |
| AAGGGGCTGATACTGGAGTT+TGG | + | chr3.4:88353697-88353716 | MS.gene41631:CDS | 50.0% | |
| ACTATTGCCGTGGTTACTGG+TGG | + | chr3.4:88352222-88352241 | MS.gene41631:CDS | 50.0% | |
| ACTGGTGGAAACCGAGGAAT+TGG | + | chr3.4:88352237-88352256 | MS.gene41631:CDS | 50.0% | |
| GAAGTTGGAAAACAGGAGGG+TGG | + | chr3.4:88353499-88353518 | MS.gene41631:CDS | 50.0% | |
| GAGGGAGAAACTGAGTGATG+TGG | + | chr3.4:88353413-88353432 | MS.gene41631:CDS | 50.0% | |
| GCAGCAGATGGCTTCATCAA+AGG | - | chr3.4:88352705-88352724 | None:intergenic | 50.0% | |
| GGTCTTGCAAGAAGGTGGTT+TGG | + | chr3.4:88352347-88352366 | MS.gene41631:CDS | 50.0% | |
| GTCCAAGGAAACTATTGCCG+TGG | + | chr3.4:88352212-88352231 | MS.gene41631:CDS | 50.0% | |
| GTTGAACAAGGGGCTGATAC+TGG | + | chr3.4:88353690-88353709 | MS.gene41631:CDS | 50.0% | |
| TCGGTTTCCACCAGTAACCA+CGG | - | chr3.4:88352232-88352251 | None:intergenic | 50.0% | |
| TTGATGAAGCCATCTGCTGC+TGG | + | chr3.4:88352705-88352724 | MS.gene41631:CDS | 50.0% | |
| ! | AGAGCAGTTTTCACCCAACC+AGG | - | chr3.4:88353645-88353664 | None:intergenic | 50.0% |
| ATACGACCACCAGCAGCAGA+TGG | - | chr3.4:88352717-88352736 | None:intergenic | 55.0% | |
| ATGAAGCCATCTGCTGCTGG+TGG | + | chr3.4:88352708-88352727 | MS.gene41631:CDS | 55.0% | |
| GAAGCTCTCTGATAGGCCAG+AGG | + | chr3.4:88353590-88353609 | MS.gene41631:CDS | 55.0% | |
| GTGGTTACTGGTGGAAACCG+AGG | + | chr3.4:88352231-88352250 | MS.gene41631:CDS | 55.0% | |
| TGGATTGAGATCAGGCGGAC+TGG | - | chr3.4:88352083-88352102 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 88351814 | 88353788 | 88351814 | ID=MS.gene41631 |
| chr3.4 | mRNA | 88351814 | 88353788 | 88351814 | ID=MS.gene41631.t1;Parent=MS.gene41631 |
| chr3.4 | exon | 88351814 | 88351899 | 88351814 | ID=MS.gene41631.t1.exon1;Parent=MS.gene41631.t1 |
| chr3.4 | CDS | 88351814 | 88351899 | 88351814 | ID=cds.MS.gene41631.t1;Parent=MS.gene41631.t1 |
| chr3.4 | exon | 88352206 | 88352464 | 88352206 | ID=MS.gene41631.t1.exon2;Parent=MS.gene41631.t1 |
| chr3.4 | CDS | 88352206 | 88352464 | 88352206 | ID=cds.MS.gene41631.t1;Parent=MS.gene41631.t1 |
| chr3.4 | exon | 88352588 | 88352782 | 88352588 | ID=MS.gene41631.t1.exon3;Parent=MS.gene41631.t1 |
| chr3.4 | CDS | 88352588 | 88352782 | 88352588 | ID=cds.MS.gene41631.t1;Parent=MS.gene41631.t1 |
| chr3.4 | exon | 88353393 | 88353788 | 88353393 | ID=MS.gene41631.t1.exon4;Parent=MS.gene41631.t1 |
| chr3.4 | CDS | 88353393 | 88353788 | 88353393 | ID=cds.MS.gene41631.t1;Parent=MS.gene41631.t1 |
| Gene Sequence |
| Protein sequence |
