Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41775.t1 | AAD56017.1 | 99 | 105 | 1 | 0 | 1 | 105 | 1 | 105 | 1.60E-13 | 85.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41775.t1 | Q9SPB5 | 99.0 | 105 | 1 | 0 | 1 | 105 | 1 | 105 | 1.1e-13 | 85.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050085 | MS.gene41775 | 0.800643 | 1.31E-48 | -1.69E-46 |
| MS.gene050317 | MS.gene41775 | 0.810535 | 1.10E-50 | -1.69E-46 |
| MS.gene050437 | MS.gene41775 | 0.80207 | 6.68E-49 | -1.69E-46 |
| MS.gene050752 | MS.gene41775 | 0.820545 | 6.48E-53 | -1.69E-46 |
| MS.gene050838 | MS.gene41775 | 0.803474 | 3.43E-49 | -1.69E-46 |
| MS.gene051003 | MS.gene41775 | 0.829206 | 5.86E-55 | -1.69E-46 |
| MS.gene051748 | MS.gene41775 | 0.80924 | 2.09E-50 | -1.69E-46 |
| MS.gene051756 | MS.gene41775 | 0.811597 | 6.47E-51 | -1.69E-46 |
| MS.gene051856 | MS.gene41775 | 0.810099 | 1.36E-50 | -1.69E-46 |
| MS.gene051903 | MS.gene41775 | 0.829063 | 6.35E-55 | -1.69E-46 |
| MS.gene052947 | MS.gene41775 | 0.817986 | 2.48E-52 | -1.69E-46 |
| MS.gene053189 | MS.gene41775 | 0.804734 | 1.87E-49 | -1.69E-46 |
| MS.gene053295 | MS.gene41775 | 0.81732 | 3.51E-52 | -1.69E-46 |
| MS.gene05344 | MS.gene41775 | -0.813414 | 2.59E-51 | -1.69E-46 |
| MS.gene053590 | MS.gene41775 | 0.814206 | 1.73E-51 | -1.69E-46 |
| MS.gene054403 | MS.gene41775 | 0.818798 | 1.62E-52 | -1.69E-46 |
| MS.gene054536 | MS.gene41775 | 0.823099 | 1.66E-53 | -1.69E-46 |
| MS.gene05469 | MS.gene41775 | 0.821085 | 4.87E-53 | -1.69E-46 |
| MS.gene056181 | MS.gene41775 | 0.802129 | 6.49E-49 | -1.69E-46 |
| MS.gene056787 | MS.gene41775 | 0.837612 | 4.69E-57 | -1.69E-46 |
| MS.gene056849 | MS.gene41775 | 0.812879 | 3.40E-51 | -1.69E-46 |
| MS.gene057234 | MS.gene41775 | 0.832881 | 7.34E-56 | -1.69E-46 |
| MS.gene057452 | MS.gene41775 | -0.811234 | 7.75E-51 | -1.69E-46 |
| MS.gene05781 | MS.gene41775 | 0.813558 | 2.41E-51 | -1.69E-46 |
| MS.gene057890 | MS.gene41775 | -0.816438 | 5.53E-52 | -1.69E-46 |
| MS.gene057999 | MS.gene41775 | 0.821792 | 3.34E-53 | -1.69E-46 |
| MS.gene058054 | MS.gene41775 | 0.825419 | 4.74E-54 | -1.69E-46 |
| MS.gene058055 | MS.gene41775 | 0.826326 | 2.88E-54 | -1.69E-46 |
| MS.gene058238 | MS.gene41775 | 0.816622 | 5.03E-52 | -1.69E-46 |
| MS.gene058646 | MS.gene41775 | 0.817007 | 4.12E-52 | -1.69E-46 |
| MS.gene058654 | MS.gene41775 | 0.828856 | 7.12E-55 | -1.69E-46 |
| MS.gene059050 | MS.gene41775 | 0.821504 | 3.90E-53 | -1.69E-46 |
| MS.gene059360 | MS.gene41775 | 0.804961 | 1.68E-49 | -1.69E-46 |
| MS.gene06010 | MS.gene41775 | 0.817573 | 3.07E-52 | -1.69E-46 |
| MS.gene06011 | MS.gene41775 | 0.813948 | 1.98E-51 | -1.69E-46 |
| MS.gene060869 | MS.gene41775 | 0.824207 | 9.15E-54 | -1.69E-46 |
| MS.gene060870 | MS.gene41775 | 0.833315 | 5.72E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41775.t1 | MTR_7g086340 | 81.452 | 124 | 8 | 1 | 1 | 124 | 1 | 109 | 2.66e-26 | 95.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 45 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTCCTTGTGCTCTTCCCTT+TGG | 0.329912 | 7.4:-21332171 | None:intergenic |
| AACCATGTTGTTCTCCTTTG+TGG | 0.347404 | 7.4:-21332075 | None:intergenic |
| AAAGGAGAGCAACATGGATT+TGG | 0.348363 | 7.4:+21332124 | MS.gene41775:CDS |
| GGAGAACAACATGGATTTGT+TGG | 0.387551 | 7.4:+21332037 | MS.gene41775:CDS |
| TGTCACTGCTGCTGCTATCA+TGG | 0.437381 | 7.4:-21332297 | None:intergenic |
| AAGATCAAGGACAAAATTCA+TGG | 0.463252 | 7.4:+21332205 | MS.gene41775:CDS |
| CTTGTCTCCTCCTCCGTGAA+GGG | 0.466823 | 7.4:-21331948 | None:intergenic |
| TCTTGTCTCCTCCTCCGTGA+AGG | 0.469459 | 7.4:-21331949 | None:intergenic |
| GCAGGAATCATGAACAAGAT+TGG | 0.500782 | 7.4:+21331920 | MS.gene41775:CDS |
| AAGATTGGTGATGCCCTTCA+CGG | 0.523694 | 7.4:+21331935 | MS.gene41775:CDS |
| AAGAAAGAGAAGAAGAAACA+TGG | 0.530177 | 7.4:+21332256 | MS.gene41775:CDS |
| GGTGATGCCCTTCACGGAGG+AGG | 0.538006 | 7.4:+21331941 | MS.gene41775:CDS |
| GGATTTGTTGGAGGACATGC+TGG | 0.539276 | 7.4:+21332049 | MS.gene41775:CDS |
| CGGAGGAGGAGACAAGAAAG+AGG | 0.550744 | 7.4:+21331955 | MS.gene41775:CDS |
| GGGATTTGCCGACAAGATCA+AGG | 0.551089 | 7.4:+21332192 | MS.gene41775:CDS |
| ATACCAAAGGGAAGAGCACA+AGG | 0.553678 | 7.4:+21332168 | MS.gene41775:CDS |
| CCCTTTGGTATCCCTCCTTG+TGG | 0.553926 | 7.4:-21332156 | None:intergenic |
| AAATAGAAATACAATATGGC+AGG | 0.556347 | 7.4:+21331902 | None:intergenic |
| GAGAAGAAGAAACATGGAGA+GGG | 0.558354 | 7.4:+21332262 | MS.gene41775:CDS |
| GGAGAACAACATGGGCATGT+AGG | 0.561384 | 7.4:+21331989 | MS.gene41775:CDS |
| CATGGAGAGGGTCATGAACA+TGG | 0.563434 | 7.4:+21332274 | MS.gene41775:CDS |
| GAACAACATGGATTTGTTGG+AGG | 0.585288 | 7.4:+21332040 | MS.gene41775:CDS |
| CCACAAGGAGGGATACCAAA+GGG | 0.586545 | 7.4:+21332156 | MS.gene41775:CDS |
| GAGCACAAAGGAGAACAACA+TGG | 0.591423 | 7.4:+21331980 | MS.gene41775:CDS |
| AAGGACAAAATTCATGGTGA+AGG | 0.593365 | 7.4:+21332211 | MS.gene41775:CDS |
| GAGCAACATGGATTTGGTCA+TGG | 0.593720 | 7.4:+21332130 | MS.gene41775:CDS |
| GAACAACATGGGCATGTAGG+AGG | 0.600098 | 7.4:+21331992 | MS.gene41775:CDS |
| AAGAAAGAGGGAGAGCACAA+AGG | 0.613945 | 7.4:+21331968 | MS.gene41775:CDS |
| ATTCATGGTGAAGGTGCAGA+TGG | 0.614174 | 7.4:+21332220 | MS.gene41775:CDS |
| CATGTAGGAGGAGAACATCA+TGG | 0.615237 | 7.4:+21332004 | MS.gene41775:CDS |
| GGAGGAGGAGACAAGAAAGA+GGG | 0.626482 | 7.4:+21331956 | MS.gene41775:CDS |
| CAAAGGGAAGAGCACAAGGA+GGG | 0.635121 | 7.4:+21332172 | MS.gene41775:CDS |
| ATTTGGTCATGGAGACCACA+AGG | 0.646260 | 7.4:+21332141 | MS.gene41775:CDS |
| GATTACAAAGGAGAGCAACA+TGG | 0.647436 | 7.4:+21332118 | MS.gene41775:CDS |
| GGTCATGGAGACCACAAGGA+GGG | 0.647519 | 7.4:+21332145 | MS.gene41775:CDS |
| ACCACAAGGAGGGATACCAA+AGG | 0.651748 | 7.4:+21332155 | MS.gene41775:CDS |
| AGAGAAGAAGAAACATGGAG+AGG | 0.656957 | 7.4:+21332261 | MS.gene41775:CDS |
| AGCACAAAGGAGAACAACAT+GGG | 0.665854 | 7.4:+21331981 | MS.gene41775:CDS |
| GAGTACAAAGGAGAACAACA+TGG | 0.667533 | 7.4:+21332028 | MS.gene41775:CDS |
| GAACATCATGGTGAGTACAA+AGG | 0.675462 | 7.4:+21332016 | MS.gene41775:CDS |
| GGACATGCTGGTGACCACAA+AGG | 0.680130 | 7.4:+21332061 | MS.gene41775:CDS |
| GACCACAAAGGAGAACAACA+TGG | 0.705961 | 7.4:+21332073 | MS.gene41775:CDS |
| TGGTCATGGAGACCACAAGG+AGG | 0.712485 | 7.4:+21332144 | MS.gene41775:CDS |
| CCAAAGGGAAGAGCACAAGG+AGG | 0.721733 | 7.4:+21332171 | MS.gene41775:CDS |
| ATTGGTGATGCCCTTCACGG+AGG | 0.747767 | 7.4:+21331938 | MS.gene41775:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGAAAGAGAAGAAGAAACA+TGG | + | chr7.4:21332256-21332275 | MS.gene41775:CDS | 30.0% | |
| AAGATCAAGGACAAAATTCA+TGG | + | chr7.4:21332205-21332224 | MS.gene41775:CDS | 30.0% | |
| ! | AATTTTGTCCTTGATCTTGT+CGG | - | chr7.4:21332203-21332222 | None:intergenic | 30.0% |
| AAGGACAAAATTCATGGTGA+AGG | + | chr7.4:21332211-21332230 | MS.gene41775:CDS | 35.0% | |
| AAAGGAGAGCAACATGGATT+TGG | + | chr7.4:21332124-21332143 | MS.gene41775:CDS | 40.0% | |
| AACCATGTTGTTCTCCTTTG+TGG | - | chr7.4:21332078-21332097 | None:intergenic | 40.0% | |
| AGAGAAGAAGAAACATGGAG+AGG | + | chr7.4:21332261-21332280 | MS.gene41775:CDS | 40.0% | |
| AGCACAAAGGAGAACAACAT+GGG | + | chr7.4:21331981-21332000 | MS.gene41775:CDS | 40.0% | |
| GAACATCATGGTGAGTACAA+AGG | + | chr7.4:21332016-21332035 | MS.gene41775:CDS | 40.0% | |
| GAGAAGAAGAAACATGGAGA+GGG | + | chr7.4:21332262-21332281 | MS.gene41775:CDS | 40.0% | |
| GAGTACAAAGGAGAACAACA+TGG | + | chr7.4:21332028-21332047 | MS.gene41775:CDS | 40.0% | |
| GATTACAAAGGAGAGCAACA+TGG | + | chr7.4:21332118-21332137 | MS.gene41775:CDS | 40.0% | |
| GCAGGAATCATGAACAAGAT+TGG | + | chr7.4:21331920-21331939 | MS.gene41775:CDS | 40.0% | |
| GGAGAACAACATGGATTTGT+TGG | + | chr7.4:21332037-21332056 | MS.gene41775:CDS | 40.0% | |
| !! | GAACAACATGGATTTGTTGG+AGG | + | chr7.4:21332040-21332059 | MS.gene41775:CDS | 40.0% |
| !!! | ATGGTTTTGTAGGAGGACAT+GGG | + | chr7.4:21332092-21332111 | MS.gene41775:CDS | 40.0% |
| !!! | GAACAACATGGTTTTGTAGG+AGG | + | chr7.4:21332085-21332104 | MS.gene41775:CDS | 40.0% |
| !!! | GGAGAACAACATGGTTTTGT+AGG | + | chr7.4:21332082-21332101 | MS.gene41775:CDS | 40.0% |
| ATTTGGTCATGGAGACCACA+AGG | + | chr7.4:21332141-21332160 | MS.gene41775:CDS | 45.0% | |
| CATGTAGGAGGAGAACATCA+TGG | + | chr7.4:21332004-21332023 | MS.gene41775:CDS | 45.0% | |
| GACCACAAAGGAGAACAACA+TGG | + | chr7.4:21332073-21332092 | MS.gene41775:CDS | 45.0% | |
| GAGCACAAAGGAGAACAACA+TGG | + | chr7.4:21331980-21331999 | MS.gene41775:CDS | 45.0% | |
| ! | AAGAAAGAGGGAGAGCACAA+AGG | + | chr7.4:21331968-21331987 | MS.gene41775:CDS | 45.0% |
| ! | ATACCAAAGGGAAGAGCACA+AGG | + | chr7.4:21332168-21332187 | MS.gene41775:CDS | 45.0% |
| ! | ATTCATGGTGAAGGTGCAGA+TGG | + | chr7.4:21332220-21332239 | MS.gene41775:CDS | 45.0% |
| ! | GAGCAACATGGATTTGGTCA+TGG | + | chr7.4:21332130-21332149 | MS.gene41775:CDS | 45.0% |
| !! | AAGATTGGTGATGCCCTTCA+CGG | + | chr7.4:21331935-21331954 | MS.gene41775:CDS | 45.0% |
| !!! | CATGGTTTTGTAGGAGGACA+TGG | + | chr7.4:21332091-21332110 | MS.gene41775:CDS | 45.0% |
| !!! | TGGTTTTGTAGGAGGACATG+GGG | + | chr7.4:21332093-21332112 | MS.gene41775:CDS | 45.0% |
| ACCACAAGGAGGGATACCAA+AGG | + | chr7.4:21332155-21332174 | MS.gene41775:CDS | 50.0% | |
| CATGGAGAGGGTCATGAACA+TGG | + | chr7.4:21332274-21332293 | MS.gene41775:CDS | 50.0% | |
| CCACAAGGAGGGATACCAAA+GGG | + | chr7.4:21332156-21332175 | MS.gene41775:CDS | 50.0% | |
| GAACAACATGGGCATGTAGG+AGG | + | chr7.4:21331992-21332011 | MS.gene41775:CDS | 50.0% | |
| GGACATGGGGGTGATTACAA+AGG | + | chr7.4:21332106-21332125 | MS.gene41775:CDS | 50.0% | |
| GGAGAACAACATGGGCATGT+AGG | + | chr7.4:21331989-21332008 | MS.gene41775:CDS | 50.0% | |
| GGAGGAGGAGACAAGAAAGA+GGG | + | chr7.4:21331956-21331975 | MS.gene41775:CDS | 50.0% | |
| GGGATTTGCCGACAAGATCA+AGG | + | chr7.4:21332192-21332211 | MS.gene41775:CDS | 50.0% | |
| TGTCACTGCTGCTGCTATCA+TGG | - | chr7.4:21332300-21332319 | None:intergenic | 50.0% | |
| ! | CAAAGGGAAGAGCACAAGGA+GGG | + | chr7.4:21332172-21332191 | MS.gene41775:CDS | 50.0% |
| !! | GGATTTGTTGGAGGACATGC+TGG | + | chr7.4:21332049-21332068 | MS.gene41775:CDS | 50.0% |
| !! | GGTTTTGTAGGAGGACATGG+GGG | + | chr7.4:21332094-21332113 | MS.gene41775:CDS | 50.0% |
| CCCTTTGGTATCCCTCCTTG+TGG | - | chr7.4:21332159-21332178 | None:intergenic | 55.0% | |
| CCTCCTTGTGCTCTTCCCTT+TGG | - | chr7.4:21332174-21332193 | None:intergenic | 55.0% | |
| CGGAGGAGGAGACAAGAAAG+AGG | + | chr7.4:21331955-21331974 | MS.gene41775:CDS | 55.0% | |
| CTTGTCTCCTCCTCCGTGAA+GGG | - | chr7.4:21331951-21331970 | None:intergenic | 55.0% | |
| GGACATGCTGGTGACCACAA+AGG | + | chr7.4:21332061-21332080 | MS.gene41775:CDS | 55.0% | |
| GGTCATGGAGACCACAAGGA+GGG | + | chr7.4:21332145-21332164 | MS.gene41775:CDS | 55.0% | |
| TCTTGTCTCCTCCTCCGTGA+AGG | - | chr7.4:21331952-21331971 | None:intergenic | 55.0% | |
| TGGTCATGGAGACCACAAGG+AGG | + | chr7.4:21332144-21332163 | MS.gene41775:CDS | 55.0% | |
| ! | CCAAAGGGAAGAGCACAAGG+AGG | + | chr7.4:21332171-21332190 | MS.gene41775:CDS | 55.0% |
| !! | ATTGGTGATGCCCTTCACGG+AGG | + | chr7.4:21331938-21331957 | MS.gene41775:CDS | 55.0% |
| GGTGATGCCCTTCACGGAGG+AGG | + | chr7.4:21331941-21331960 | MS.gene41775:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 21331917 | 21332327 | 21331917 | ID=MS.gene41775 |
| chr7.4 | mRNA | 21331917 | 21332327 | 21331917 | ID=MS.gene41775.t1;Parent=MS.gene41775 |
| chr7.4 | exon | 21331917 | 21332327 | 21331917 | ID=MS.gene41775.t1.exon1;Parent=MS.gene41775.t1 |
| chr7.4 | CDS | 21331917 | 21332327 | 21331917 | ID=cds.MS.gene41775.t1;Parent=MS.gene41775.t1 |
| Gene Sequence |
| Protein sequence |
