Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46252.t1 | XP_013461302.1 | 97.6 | 124 | 3 | 0 | 1 | 124 | 1 | 124 | 6.90E-54 | 221.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46252.t1 | Q5Q995 | 39.5 | 119 | 64 | 2 | 4 | 114 | 5 | 123 | 1.1e-12 | 75.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46252.t1 | I3T7J3 | 97.6 | 124 | 3 | 0 | 1 | 124 | 1 | 124 | 5.0e-54 | 221.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene46252.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049411 | MS.gene46252 | 0.814323 | 1.63E-51 | -1.69E-46 |
| MS.gene049680 | MS.gene46252 | 0.803045 | 4.20E-49 | -1.69E-46 |
| MS.gene049807 | MS.gene46252 | 0.81401 | 1.92E-51 | -1.69E-46 |
| MS.gene049887 | MS.gene46252 | 0.819184 | 1.33E-52 | -1.69E-46 |
| MS.gene050277 | MS.gene46252 | 0.815489 | 9.01E-52 | -1.69E-46 |
| MS.gene050320 | MS.gene46252 | 0.812859 | 3.43E-51 | -1.69E-46 |
| MS.gene050437 | MS.gene46252 | 0.837402 | 5.31E-57 | -1.69E-46 |
| MS.gene050752 | MS.gene46252 | 0.910371 | 2.02E-82 | -1.69E-46 |
| MS.gene050838 | MS.gene46252 | 0.86179 | 8.01E-64 | -1.69E-46 |
| MS.gene050955 | MS.gene46252 | 0.908176 | 2.28E-81 | -1.69E-46 |
| MS.gene051003 | MS.gene46252 | 0.842214 | 2.96E-58 | -1.69E-46 |
| MS.gene051234 | MS.gene46252 | 0.865718 | 4.82E-65 | -1.69E-46 |
| MS.gene05134 | MS.gene46252 | 0.811021 | 8.62E-51 | -1.69E-46 |
| MS.gene051746 | MS.gene46252 | 0.856447 | 3.20E-62 | -1.69E-46 |
| MS.gene051748 | MS.gene46252 | 0.851141 | 1.08E-60 | -1.69E-46 |
| MS.gene051850 | MS.gene46252 | 0.814124 | 1.81E-51 | -1.69E-46 |
| MS.gene051885 | MS.gene46252 | 0.812151 | 4.90E-51 | -1.69E-46 |
| MS.gene051903 | MS.gene46252 | 0.863336 | 2.68E-64 | -1.69E-46 |
| MS.gene052133 | MS.gene46252 | 0.804785 | 1.83E-49 | -1.69E-46 |
| MS.gene052335 | MS.gene46252 | 0.820898 | 5.38E-53 | -1.69E-46 |
| MS.gene052479 | MS.gene46252 | 0.817492 | 3.21E-52 | -1.69E-46 |
| MS.gene052947 | MS.gene46252 | 0.883125 | 5.85E-71 | -1.69E-46 |
| MS.gene053109 | MS.gene46252 | 0.807441 | 5.05E-50 | -1.69E-46 |
| MS.gene053581 | MS.gene46252 | 0.820767 | 5.76E-53 | -1.69E-46 |
| MS.gene053952 | MS.gene46252 | 0.811286 | 7.55E-51 | -1.69E-46 |
| MS.gene054250 | MS.gene46252 | 0.812304 | 4.53E-51 | -1.69E-46 |
| MS.gene054403 | MS.gene46252 | 0.913402 | 6.41E-84 | -1.69E-46 |
| MS.gene055468 | MS.gene46252 | 0.804112 | 2.53E-49 | -1.69E-46 |
| MS.gene055555 | MS.gene46252 | 0.857822 | 1.26E-62 | -1.69E-46 |
| MS.gene056043 | MS.gene46252 | -0.809508 | 1.83E-50 | -1.69E-46 |
| MS.gene056075 | MS.gene46252 | 0.872837 | 2.34E-67 | -1.69E-46 |
| MS.gene056631 | MS.gene46252 | 0.824467 | 7.94E-54 | -1.69E-46 |
| MS.gene057102 | MS.gene46252 | 0.813701 | 2.24E-51 | -1.69E-46 |
| MS.gene057234 | MS.gene46252 | 0.805396 | 1.36E-49 | -1.69E-46 |
| MS.gene057694 | MS.gene46252 | 0.804082 | 2.56E-49 | -1.69E-46 |
| MS.gene057705 | MS.gene46252 | 0.875173 | 3.80E-68 | -1.69E-46 |
| MS.gene058074 | MS.gene46252 | 0.809439 | 1.89E-50 | -1.69E-46 |
| MS.gene058245 | MS.gene46252 | 0.805151 | 1.53E-49 | -1.69E-46 |
| MS.gene058852 | MS.gene46252 | 0.808911 | 2.45E-50 | -1.69E-46 |
| MS.gene059227 | MS.gene46252 | 0.839612 | 1.43E-57 | -1.69E-46 |
| MS.gene06001 | MS.gene46252 | 0.840402 | 8.89E-58 | -1.69E-46 |
| MS.gene06011 | MS.gene46252 | 0.801031 | 1.09E-48 | -1.69E-46 |
| MS.gene060917 | MS.gene46252 | 0.802288 | 6.02E-49 | -1.69E-46 |
| MS.gene06118 | MS.gene46252 | 0.823855 | 1.11E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46252.t1 | MTR_3g088525 | 97.581 | 124 | 3 | 0 | 1 | 124 | 1 | 124 | 1.31e-78 | 238 |
| MS.gene46252.t1 | MTR_3g088515 | 65.761 | 184 | 19 | 1 | 121 | 260 | 7 | 190 | 8.05e-71 | 223 |
| MS.gene46252.t1 | MTR_1g046340 | 80.180 | 111 | 22 | 0 | 126 | 236 | 1 | 111 | 3.50e-57 | 190 |
| MS.gene46252.t1 | MTR_1g046320 | 71.560 | 109 | 31 | 0 | 126 | 234 | 1 | 109 | 1.71e-46 | 162 |
| MS.gene46252.t1 | MTR_1g046200 | 71.963 | 107 | 30 | 0 | 130 | 236 | 2 | 108 | 1.65e-45 | 159 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46252.t1 | AT1G77350 | 74.590 | 122 | 30 | 1 | 1 | 121 | 1 | 122 | 1.44e-55 | 179 |
| MS.gene46252.t1 | AT1G77350 | 74.590 | 122 | 30 | 1 | 1 | 121 | 1 | 122 | 1.44e-55 | 179 |
Find 83 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGCAGTGTTGGTCATAATT+TGG | 0.253758 | 3.1:+71430999 | None:intergenic |
| TGAGAAGCCATGGCGGGGTC+TGG | 0.324778 | 3.1:-71435349 | None:intergenic |
| CAGTTAGGAGCACAAGAAAT+AGG | 0.332990 | 3.1:+71435206 | None:intergenic |
| ACTTTCCCGGCGGTGGAAAC+GGG | 0.333345 | 3.1:-71427826 | MS.gene46252:CDS |
| AACTTTCCCGGCGGTGGAAA+CGG | 0.347641 | 3.1:-71427827 | MS.gene46252:CDS |
| CAGAGAAGAAAATTCATTAG+AGG | 0.353395 | 3.1:-71430588 | MS.gene46252:CDS |
| TGCCATATTGCGTGTCCGCC+TGG | 0.359320 | 3.1:+71427986 | None:intergenic |
| ATGAGCTTCACAAAAGCAAT+TGG | 0.382357 | 3.1:+71430731 | None:intergenic |
| GAGACAGCCGGTGCAAAATC+TGG | 0.384994 | 3.1:-71434531 | MS.gene46252:CDS |
| TGAATGCAGTTTCCTGTTCT+CGG | 0.393893 | 3.1:-71433345 | MS.gene46252:intron |
| CGCGTCAAAGCACATTCAAT+TGG | 0.401347 | 3.1:-71430647 | MS.gene46252:CDS |
| AGCTCAAACGGAGGCGGTGG+TGG | 0.412381 | 3.1:-71427950 | MS.gene46252:CDS |
| ACTTTCCTAGTGGTGGTAAA+GGG | 0.412711 | 3.1:-71427916 | MS.gene46252:CDS |
| GGCCAGGCGGACACGCAATA+TGG | 0.417457 | 3.1:-71427988 | MS.gene46252:CDS |
| GTCATAAACTTTCCCGGCGG+TGG | 0.417743 | 3.1:-71427833 | MS.gene46252:CDS |
| GCAAAATCTGGCTGGGGTGC+TGG | 0.425345 | 3.1:-71434519 | MS.gene46252:intron |
| TCGGAATTATTCACAATACT+TGG | 0.430748 | 3.1:-71435247 | MS.gene46252:CDS |
| GAGAAGCCATGGCGGGGTCT+GGG | 0.434467 | 3.1:-71435348 | None:intergenic |
| AACTTTCCTAGTGGTGGTAA+AGG | 0.439112 | 3.1:-71427917 | MS.gene46252:CDS |
| GTGTTGATGGCTATATAGTT+TGG | 0.439892 | 3.1:-71428009 | MS.gene46252:CDS |
| CTCGGCTGTGTTGCTCTATG+AGG | 0.447834 | 3.1:-71433327 | MS.gene46252:CDS |
| GTATGTGTTGTGTTGATTTG+TGG | 0.448016 | 3.1:+71429481 | None:intergenic |
| GCAAATGTCATAAACTTTCC+CGG | 0.460290 | 3.1:-71427839 | MS.gene46252:CDS |
| TTGCTATGAGAAGCCATGGC+GGG | 0.473545 | 3.1:-71435355 | None:intergenic |
| AGAGCAACACAGCCGAGAAC+AGG | 0.475210 | 3.1:+71433333 | None:intergenic |
| AGCCGGTGCAAAATCTGGCT+GGG | 0.475968 | 3.1:-71434526 | MS.gene46252:CDS |
| CAGCCGGTGCAAAATCTGGC+TGG | 0.479798 | 3.1:-71434527 | MS.gene46252:CDS |
| GCCTCCGTTTGAGCTTCCAC+TGG | 0.480548 | 3.1:+71427958 | None:intergenic |
| AGCACCAGTGGAAGCTCAAA+CGG | 0.486563 | 3.1:-71427962 | MS.gene46252:CDS |
| ATGAAAGCAGAAAAGAAGAA+AGG | 0.488071 | 3.1:-71430266 | MS.gene46252:intron |
| CTCTTCCTCTATGATCCCTT+AGG | 0.492258 | 3.1:+71430675 | None:intergenic |
| TTGTGGCAATATGTGATGAT+AGG | 0.496982 | 3.1:+71429498 | None:intergenic |
| GATGACAATGAATAGAAGAA+AGG | 0.500365 | 3.1:+71435309 | None:intergenic |
| TCTCATAATGCTTCTCTATG+AGG | 0.504327 | 3.1:+71430948 | None:intergenic |
| TATGAGGTTAACAAGCTCTC+TGG | 0.505681 | 3.1:-71433311 | MS.gene46252:CDS |
| GGGATCATAGAGGAAGAGAT+TGG | 0.510517 | 3.1:-71430670 | MS.gene46252:CDS |
| CTTTCCCGGCGGTGGAAACG+GGG | 0.511813 | 3.1:-71427825 | MS.gene46252:CDS |
| GGAAGCTCAAACGGAGGCGG+TGG | 0.512147 | 3.1:-71427953 | MS.gene46252:CDS |
| ATCATAAACTTTCCTAGTGG+TGG | 0.514983 | 3.1:-71427923 | MS.gene46252:CDS |
| AAGCACATTCAATTGGTGGC+AGG | 0.516729 | 3.1:-71430640 | MS.gene46252:CDS |
| AAGAAGAAAGGAGTTGACAA+GGG | 0.522584 | 3.1:-71430254 | MS.gene46252:CDS |
| TAGAAGAAAGGAATACATCA+TGG | 0.524516 | 3.1:+71435321 | None:intergenic |
| AGAGAAGCATTATGAGAGAA+TGG | 0.527328 | 3.1:-71430942 | MS.gene46252:intron |
| GATGGCTATATAGTTTGGCC+AGG | 0.530422 | 3.1:-71428004 | MS.gene46252:CDS |
| GAATTATTCACAATACTTGG+AGG | 0.534232 | 3.1:-71435244 | MS.gene46252:CDS |
| AATTATTCACAATACTTGGA+GGG | 0.538766 | 3.1:-71435243 | MS.gene46252:CDS |
| AAAGAAGAAAGGAGTTGACA+AGG | 0.542225 | 3.1:-71430255 | MS.gene46252:CDS |
| GAGGGACATAAGTCAAGCGT+TGG | 0.544112 | 3.1:-71427782 | MS.gene46252:CDS |
| ACCATGTAGTGATGCATACT+CGG | 0.544399 | 3.1:+71434214 | None:intergenic |
| TGAGCTTCACAAAAGCAATT+GGG | 0.545679 | 3.1:+71430732 | None:intergenic |
| GTCAAAGCACATTCAATTGG+TGG | 0.546694 | 3.1:-71430644 | MS.gene46252:CDS |
| ACCAGTGGAAGCTCAAACGG+AGG | 0.551732 | 3.1:-71427959 | MS.gene46252:CDS |
| TTTCAATATGTGTGAAAACA+AGG | 0.554136 | 3.1:+71430555 | None:intergenic |
| GAGGAACACCTTGGCAGTGT+TGG | 0.557953 | 3.1:+71430988 | None:intergenic |
| GCCGGTGCAAAATCTGGCTG+GGG | 0.558097 | 3.1:-71434525 | MS.gene46252:CDS |
| ATTTGTAGTTGGTGAGACAC+AGG | 0.559962 | 3.1:-71428050 | MS.gene46252:intron |
| AGCTCATGCTTTCGAAACAA+AGG | 0.561664 | 3.1:-71430715 | MS.gene46252:CDS |
| GGAAGACTTTGAAGAAGTTG+AGG | 0.564880 | 3.1:+71430969 | None:intergenic |
| CCGTGGGAAACTTGCATCGT+CGG | 0.565118 | 3.1:-71435266 | MS.gene46252:CDS |
| GCTCATGCTTTCGAAACAAA+GGG | 0.566004 | 3.1:-71430714 | MS.gene46252:CDS |
| TGCTATGAGAAGCCATGGCG+GGG | 0.568516 | 3.1:-71435354 | None:intergenic |
| AGTGGAAGCTCAAACGGAGG+CGG | 0.581193 | 3.1:-71427956 | MS.gene46252:CDS |
| CTTTCCTAGTGGTGGTAAAG+GGG | 0.585504 | 3.1:-71427915 | MS.gene46252:CDS |
| CACTTCAAGAAATGTACCGT+GGG | 0.587600 | 3.1:-71435282 | MS.gene46252:CDS |
| TTTCCTAGTGGTGGTAAAGG+GGG | 0.588254 | 3.1:-71427914 | MS.gene46252:CDS |
| GGCTATATAGTTTGGCCAGG+CGG | 0.594243 | 3.1:-71428001 | MS.gene46252:CDS |
| GAAGAAGTTGAGGAACACCT+TGG | 0.598409 | 3.1:+71430979 | None:intergenic |
| ACCGAGTATGCATCACTACA+TGG | 0.601750 | 3.1:-71434215 | MS.gene46252:intron |
| TTTCCCGGCGGTGGAAACGG+GGG | 0.606258 | 3.1:-71427824 | MS.gene46252:CDS |
| TTTATCCTAAGGGATCATAG+AGG | 0.616153 | 3.1:-71430680 | MS.gene46252:CDS |
| GGAACTCCCAGACCCCGCCA+TGG | 0.620572 | 3.1:+71435342 | None:intergenic |
| AATTATGACCAACACTGCCA+AGG | 0.625272 | 3.1:-71430996 | MS.gene46252:CDS |
| GGTACATTTCTTGAAGTGAG+AGG | 0.637005 | 3.1:+71435287 | None:intergenic |
| GCAATATGGCAAAGCACCAG+TGG | 0.640117 | 3.1:-71427974 | MS.gene46252:CDS |
| CCCGGAAGTGGAAAACGGGG+TGG | 0.655848 | 3.1:-71427866 | MS.gene46252:CDS |
| ACAACACATACCGAAAAGAG+AGG | 0.656927 | 3.1:-71429469 | MS.gene46252:CDS |
| CCGACGATGCAAGTTTCCCA+CGG | 0.661665 | 3.1:+71435266 | None:intergenic |
| GGAGTCACTTGTTGAAACAG+CGG | 0.662603 | 3.1:+71433286 | None:intergenic |
| ATTATCATAAACTTTCCTAG+TGG | 0.664481 | 3.1:-71427926 | MS.gene46252:CDS |
| TCACTTCAAGAAATGTACCG+TGG | 0.674064 | 3.1:-71435283 | MS.gene46252:CDS |
| TTTCCCGGAAGTGGAAAACG+GGG | 0.695053 | 3.1:-71427869 | MS.gene46252:CDS |
| ATGTAGTGATGCATACTCGG+TGG | 0.725698 | 3.1:+71434217 | None:intergenic |
| AATGTCATAAACTTTCCCGG+CGG | 0.795660 | 3.1:-71427836 | MS.gene46252:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 71427760 | 71435362 | 71427760 | ID=MS.gene46252 |
| chr3.1 | mRNA | 71427760 | 71435362 | 71427760 | ID=MS.gene46252.t1;Parent=MS.gene46252 |
| chr3.1 | exon | 71435207 | 71435362 | 71435207 | ID=MS.gene46252.t1.exon1;Parent=MS.gene46252.t1 |
| chr3.1 | CDS | 71435207 | 71435362 | 71435207 | ID=cds.MS.gene46252.t1;Parent=MS.gene46252.t1 |
| chr3.1 | exon | 71434520 | 71434571 | 71434520 | ID=MS.gene46252.t1.exon2;Parent=MS.gene46252.t1 |
| chr3.1 | CDS | 71434520 | 71434571 | 71434520 | ID=cds.MS.gene46252.t1;Parent=MS.gene46252.t1 |
| chr3.1 | exon | 71434216 | 71434282 | 71434216 | ID=MS.gene46252.t1.exon3;Parent=MS.gene46252.t1 |
| chr3.1 | CDS | 71434216 | 71434282 | 71434216 | ID=cds.MS.gene46252.t1;Parent=MS.gene46252.t1 |
| chr3.1 | exon | 71433270 | 71433358 | 71433270 | ID=MS.gene46252.t1.exon4;Parent=MS.gene46252.t1 |
| chr3.1 | CDS | 71433270 | 71433358 | 71433270 | ID=cds.MS.gene46252.t1;Parent=MS.gene46252.t1 |
| chr3.1 | exon | 71430943 | 71431025 | 71430943 | ID=MS.gene46252.t1.exon5;Parent=MS.gene46252.t1 |
| chr3.1 | CDS | 71430943 | 71431025 | 71430943 | ID=cds.MS.gene46252.t1;Parent=MS.gene46252.t1 |
| chr3.1 | exon | 71430499 | 71430759 | 71430499 | ID=MS.gene46252.t1.exon6;Parent=MS.gene46252.t1 |
| chr3.1 | CDS | 71430499 | 71430759 | 71430499 | ID=cds.MS.gene46252.t1;Parent=MS.gene46252.t1 |
| chr3.1 | exon | 71430207 | 71430278 | 71430207 | ID=MS.gene46252.t1.exon7;Parent=MS.gene46252.t1 |
| chr3.1 | CDS | 71430207 | 71430278 | 71430207 | ID=cds.MS.gene46252.t1;Parent=MS.gene46252.t1 |
| chr3.1 | exon | 71429461 | 71429545 | 71429461 | ID=MS.gene46252.t1.exon8;Parent=MS.gene46252.t1 |
| chr3.1 | CDS | 71429461 | 71429545 | 71429461 | ID=cds.MS.gene46252.t1;Parent=MS.gene46252.t1 |
| chr3.1 | exon | 71427760 | 71428064 | 71427760 | ID=MS.gene46252.t1.exon9;Parent=MS.gene46252.t1 |
| chr3.1 | CDS | 71427760 | 71428064 | 71427760 | ID=cds.MS.gene46252.t1;Parent=MS.gene46252.t1 |
| Gene Sequence |
| Protein sequence |
