AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene47926


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47926.t1 MTR_8g089300 95.876 194 8 0 1 194 1 194 5.61e-127 355
MS.gene47926.t1 MTR_0097s0070 75.773 194 46 1 1 193 1 194 1.98e-97 281
MS.gene47926.t1 MTR_4g118810 36.111 144 90 2 28 170 35 177 3.66e-25 97.1
MS.gene47926.t1 MTR_0004s0210 35.185 162 89 2 28 189 10 155 1.11e-23 92.4
MS.gene47926.t1 MTR_2g020060 35.260 173 92 4 30 194 27 187 2.13e-23 92.4
MS.gene47926.t1 MTR_7g108130 32.778 180 114 3 16 194 18 191 2.24e-23 92.4
MS.gene47926.t1 MTR_4g081880 29.630 189 106 6 9 184 6 180 2.72e-16 73.6
MS.gene47926.t1 MTR_4g118800 30.159 126 87 1 64 188 56 181 2.81e-16 73.6
MS.gene47926.t1 MTR_7g011090 28.387 155 104 1 30 184 32 179 8.16e-16 72.4
MS.gene47926.t1 MTR_5g041900 28.387 155 104 1 30 184 32 179 5.18e-13 64.7
MS.gene47926.t1 MTR_1g058540 29.861 144 92 3 30 172 42 177 5.30e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47926.t1 AT5G44550 53.714 175 81 0 19 193 11 185 5.24e-61 189
MS.gene47926.t1 AT4G20390 55.491 173 76 1 23 194 14 186 2.38e-55 174
MS.gene47926.t1 AT4G15610 33.803 142 89 3 30 170 33 170 1.48e-20 85.1
MS.gene47926.t1 AT1G03700 30.189 159 95 2 28 186 11 153 1.09e-19 82.0
MS.gene47926.t1 AT4G15630 31.481 162 109 2 28 188 29 189 1.23e-19 82.4
MS.gene47926.t1 AT1G14160 32.121 165 102 2 30 194 55 209 3.87e-19 81.6
MS.gene47926.t1 AT4G15620 29.341 167 116 2 23 188 24 189 1.12e-18 80.1
MS.gene47926.t1 AT5G15290 30.114 176 108 4 1 171 1 166 1.86e-17 76.6
MS.gene47926.t1 AT3G06390 31.061 132 87 2 31 161 42 170 3.20e-14 68.6
MS.gene47926.t1 AT4G03540 28.244 131 78 2 43 173 26 140 1.53e-13 65.9

Find 45 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTGTCAGTCTTCATAATT+TGG 0.186212 4.1:+11465690 MS.gene47926:CDS
GCCTTAATAGCTTCTTTCAT+TGG 0.241559 4.1:+11466027 MS.gene47926:CDS
GCAGTAAGAGGAACTGTAAT+TGG 0.269342 4.1:-11465474 None:intergenic
AAAAGGATCCAATCCTTCTT+TGG 0.280238 4.1:-11465348 None:intergenic
CCCAATGAAAGAAGCTATTA+AGG 0.288413 4.1:-11466028 None:intergenic
TCCTTCTTTGGTTTGGTCTC+AGG 0.290070 4.1:-11465336 None:intergenic
AGCAACATTTATGTCAGAAT+TGG 0.314088 4.1:+11465927 MS.gene47926:CDS
GGTAGCAACTACAAAGCTTT+TGG 0.318946 4.1:-11465439 None:intergenic
ATGATAGCAATGGATATATT+AGG 0.342029 4.1:+11465715 MS.gene47926:CDS
CCTTAATAGCTTCTTTCATT+GGG 0.354084 4.1:+11466028 MS.gene47926:CDS
ATGGTAATTCACATGCAAAA+TGG 0.384012 4.1:+11465956 MS.gene47926:CDS
TTGGGTGAATTAGATGGCTT+TGG 0.384488 4.1:+11465879 MS.gene47926:intron
GTTAATTGCGATTTAGTTTG+AGG 0.384618 4.1:-11466095 None:intergenic
GATCCAATCCTTCTTTGGTT+TGG 0.385867 4.1:-11465343 None:intergenic
CAGTAAGAGGAACTGTAATT+GGG 0.394934 4.1:-11465473 None:intergenic
TAGTTTGAGGAGTTTGTTGA+TGG 0.399777 4.1:-11466082 None:intergenic
GCAACATTTATGTCAGAATT+GGG 0.419599 4.1:+11465928 MS.gene47926:CDS
GACTCAAATTTCATAACAAA+GGG 0.455425 4.1:+11465737 MS.gene47926:CDS
TCCTGAGACCAAACCAAAGA+AGG 0.459469 4.1:+11465335 MS.gene47926:CDS
TGCTGAAACTTGGCAGTAAG+AGG 0.478796 4.1:-11465486 None:intergenic
ACCACCACCTCTGTTGCAAT+AGG 0.484004 4.1:-11466004 None:intergenic
CAACCACCCTAAGGGACAAA+AGG 0.486521 4.1:-11465365 None:intergenic
TTATGGACTAACACAAAAGC+TGG 0.487148 4.1:-11465513 None:intergenic
AGCTGGAGTATGCTGAAACT+TGG 0.488766 4.1:-11465496 None:intergenic
AGACCAAACCAAAGAAGGAT+TGG 0.490966 4.1:+11465340 MS.gene47926:CDS
AGCTTTGTAGTTGCTACCAT+TGG 0.493742 4.1:+11465444 MS.gene47926:CDS
GGACTCAAATTTCATAACAA+AGG 0.497208 4.1:+11465736 MS.gene47926:CDS
ATGTCAGAATTGGGAAGGAA+TGG 0.507856 4.1:+11465937 MS.gene47926:CDS
TACACTATCACAATGGCCTC+AGG 0.512074 4.1:+11465276 None:intergenic
TAATTTGGTTATGATAGCAA+TGG 0.531106 4.1:+11465705 MS.gene47926:CDS
ATGAAGACTGACAATTCCAT+TGG 0.535909 4.1:-11465683 None:intergenic
TCCTATTGCAACAGAGGTGG+TGG 0.560694 4.1:+11466003 MS.gene47926:CDS
AGGTACTTTGTGGTAGCCAA+TGG 0.565693 4.1:+11465667 MS.gene47926:intron
GCTCTAGCATCAAGTGGAGA+TGG 0.573326 4.1:+11465901 MS.gene47926:CDS
TGTAATTGGGGTGCTACCAA+TGG 0.595649 4.1:-11465460 None:intergenic
TTTGAGTCCTATTGCAACAG+AGG 0.599496 4.1:+11465997 MS.gene47926:CDS
CATTTATGTCAGAATTGGGA+AGG 0.599985 4.1:+11465932 MS.gene47926:CDS
ATACTGCAATCAGTGCAAGT+TGG 0.603365 4.1:-11465762 None:intergenic
GAGTCCTATTGCAACAGAGG+TGG 0.611119 4.1:+11466000 MS.gene47926:CDS
AACAAGTGTAGCTGATACAG+TGG 0.613356 4.1:-11465397 None:intergenic
ACATCACTGTAATAATGAGT+AGG 0.627687 4.1:-11466059 None:intergenic
GTGAGAATACAGGTACTTTG+TGG 0.628246 4.1:+11465657 MS.gene47926:intron
GCTTTGGCTCTAGCATCAAG+TGG 0.633064 4.1:+11465895 MS.gene47926:CDS
AGTAAGAGGAACTGTAATTG+GGG 0.643245 4.1:-11465472 None:intergenic
ACTGATTGCAGTATTTGACA+CGG 0.674366 4.1:+11465771 MS.gene47926:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAAATTAGAGAGATTTTA+TGG - chr4.1:11465533-11465552 None:intergenic 15.0%
!! TAACTTAAATGATCAATTAG+AGG - chr4.1:11465813-11465832 None:intergenic 20.0%
! AATGAAGAAAAAATAGAGTC+TGG + chr4.1:11465300-11465319 MS.gene47926:CDS 25.0%
! AATTGTCAGTCTTCATAATT+TGG + chr4.1:11465690-11465709 MS.gene47926:CDS 25.0%
! ATGATAGCAATGGATATATT+AGG + chr4.1:11465715-11465734 MS.gene47926:CDS 25.0%
! GACTCAAATTTCATAACAAA+GGG + chr4.1:11465737-11465756 MS.gene47926:CDS 25.0%
! TAATTTGGTTATGATAGCAA+TGG + chr4.1:11465705-11465724 MS.gene47926:CDS 25.0%
! TCACCACAAATTAAATCATA+AGG - chr4.1:11465641-11465660 None:intergenic 25.0%
!!! TATTTTTTCTTCATTTCCTG+AGG - chr4.1:11465295-11465314 None:intergenic 25.0%
!!! TCACCTTATGATTTAATTTG+TGG + chr4.1:11465635-11465654 MS.gene47926:intron 25.0%
ACATCACTGTAATAATGAGT+AGG - chr4.1:11466062-11466081 None:intergenic 30.0%
AGCAACATTTATGTCAGAAT+TGG + chr4.1:11465927-11465946 MS.gene47926:CDS 30.0%
ATGGTAATTCACATGCAAAA+TGG + chr4.1:11465956-11465975 MS.gene47926:CDS 30.0%
CCTTAATAGCTTCTTTCATT+GGG + chr4.1:11466028-11466047 MS.gene47926:CDS 30.0%
GATTAATTGGGTGAATTAGA+TGG + chr4.1:11465873-11465892 MS.gene47926:intron 30.0%
GCAACATTTATGTCAGAATT+GGG + chr4.1:11465928-11465947 MS.gene47926:CDS 30.0%
GGACTCAAATTTCATAACAA+AGG + chr4.1:11465736-11465755 MS.gene47926:CDS 30.0%
!! TAGATACAGAAGTGTTAGAA+AGG - chr4.1:11465556-11465575 None:intergenic 30.0%
!! TTAATTTGTGGTGAGAATAC+AGG + chr4.1:11465647-11465666 MS.gene47926:intron 30.0%
AAAAGGATCCAATCCTTCTT+TGG - chr4.1:11465351-11465370 None:intergenic 35.0%
ACTGATTGCAGTATTTGACA+CGG + chr4.1:11465771-11465790 MS.gene47926:CDS 35.0%
AGTAAGAGGAACTGTAATTG+GGG - chr4.1:11465475-11465494 None:intergenic 35.0%
ATGAAGACTGACAATTCCAT+TGG - chr4.1:11465686-11465705 None:intergenic 35.0%
CAGTAAGAGGAACTGTAATT+GGG - chr4.1:11465476-11465495 None:intergenic 35.0%
CATTTATGTCAGAATTGGGA+AGG + chr4.1:11465932-11465951 MS.gene47926:CDS 35.0%
CCCAATGAAAGAAGCTATTA+AGG - chr4.1:11466031-11466050 None:intergenic 35.0%
GCCTTAATAGCTTCTTTCAT+TGG + chr4.1:11466027-11466046 MS.gene47926:CDS 35.0%
! TTATGGACTAACACAAAAGC+TGG - chr4.1:11465516-11465535 None:intergenic 35.0%
!! TAGTTTGAGGAGTTTGTTGA+TGG - chr4.1:11466085-11466104 None:intergenic 35.0%
AACAAGTGTAGCTGATACAG+TGG - chr4.1:11465400-11465419 None:intergenic 40.0%
AGACCAAACCAAAGAAGGAT+TGG + chr4.1:11465340-11465359 MS.gene47926:CDS 40.0%
ATACTGCAATCAGTGCAAGT+TGG - chr4.1:11465765-11465784 None:intergenic 40.0%
ATGTCAGAATTGGGAAGGAA+TGG + chr4.1:11465937-11465956 MS.gene47926:CDS 40.0%
CAAAAATGCAACCACCCTAA+GGG - chr4.1:11465376-11465395 None:intergenic 40.0%
GATCCAATCCTTCTTTGGTT+TGG - chr4.1:11465346-11465365 None:intergenic 40.0%
GCAGTAAGAGGAACTGTAAT+TGG - chr4.1:11465477-11465496 None:intergenic 40.0%
GTGAGAATACAGGTACTTTG+TGG + chr4.1:11465657-11465676 MS.gene47926:intron 40.0%
TTGGGTGAATTAGATGGCTT+TGG + chr4.1:11465879-11465898 MS.gene47926:intron 40.0%
TTTGAGTCCTATTGCAACAG+AGG + chr4.1:11465997-11466016 MS.gene47926:CDS 40.0%
! AGCTTTGTAGTTGCTACCAT+TGG + chr4.1:11465444-11465463 MS.gene47926:CDS 40.0%
! ATTGGATCCTTTTGTCCCTT+AGG + chr4.1:11465358-11465377 MS.gene47926:CDS 40.0%
! GGTAGCAACTACAAAGCTTT+TGG - chr4.1:11465442-11465461 None:intergenic 40.0%
! TTGGATCCTTTTGTCCCTTA+GGG + chr4.1:11465359-11465378 MS.gene47926:CDS 40.0%
AGCTGGAGTATGCTGAAACT+TGG - chr4.1:11465499-11465518 None:intergenic 45.0%
AGGTACTTTGTGGTAGCCAA+TGG + chr4.1:11465667-11465686 MS.gene47926:intron 45.0%
CCAAAAATGCAACCACCCTA+AGG - chr4.1:11465377-11465396 None:intergenic 45.0%
TCCTGAGACCAAACCAAAGA+AGG + chr4.1:11465335-11465354 MS.gene47926:CDS 45.0%
TGCTGAAACTTGGCAGTAAG+AGG - chr4.1:11465489-11465508 None:intergenic 45.0%
! CCTTAGGGTGGTTGCATTTT+TGG + chr4.1:11465374-11465393 MS.gene47926:CDS 45.0%
! TGTAATTGGGGTGCTACCAA+TGG - chr4.1:11465463-11465482 None:intergenic 45.0%
!! TCCTTCTTTGGTTTGGTCTC+AGG - chr4.1:11465339-11465358 None:intergenic 45.0%
!!! ATTATATTTTTTTGATTAAT+TGG + chr4.1:11465860-11465879 MS.gene47926:intron 5.0%
!!! TTATATTTTTTTGATTAATT+GGG + chr4.1:11465861-11465880 MS.gene47926:intron 5.0%
ACCACCACCTCTGTTGCAAT+AGG - chr4.1:11466007-11466026 None:intergenic 50.0%
CAACCACCCTAAGGGACAAA+AGG - chr4.1:11465368-11465387 None:intergenic 50.0%
GAGTCCTATTGCAACAGAGG+TGG + chr4.1:11466000-11466019 MS.gene47926:CDS 50.0%
GCTCTAGCATCAAGTGGAGA+TGG + chr4.1:11465901-11465920 MS.gene47926:CDS 50.0%
TCCTATTGCAACAGAGGTGG+TGG + chr4.1:11466003-11466022 MS.gene47926:CDS 50.0%
! GATCCTTTTGTCCCTTAGGG+TGG + chr4.1:11465362-11465381 MS.gene47926:CDS 50.0%
!! GCTTTGGCTCTAGCATCAAG+TGG + chr4.1:11465895-11465914 MS.gene47926:CDS 50.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 11465288 11466116 11465288 ID=MS.gene47926
chr4.1 mRNA 11465288 11466116 11465288 ID=MS.gene47926.t1;Parent=MS.gene47926
chr4.1 exon 11465288 11465523 11465288 ID=MS.gene47926.t1.exon1;Parent=MS.gene47926.t1
chr4.1 CDS 11465288 11465523 11465288 ID=cds.MS.gene47926.t1;Parent=MS.gene47926.t1
chr4.1 exon 11465669 11465792 11465669 ID=MS.gene47926.t1.exon2;Parent=MS.gene47926.t1
chr4.1 CDS 11465669 11465792 11465669 ID=cds.MS.gene47926.t1;Parent=MS.gene47926.t1
chr4.1 exon 11465892 11466116 11465892 ID=MS.gene47926.t1.exon3;Parent=MS.gene47926.t1
chr4.1 CDS 11465892 11466116 11465892 ID=cds.MS.gene47926.t1;Parent=MS.gene47926.t1
Gene Sequence

>MS.gene47926

ATGGCCTCAGGAAATGAAGAAAAAATAGAGTCTGGTTTCAATGTTGTTCCTGAGACCAAACCAAAGAAGGATTGGATCCTTTTGTCCCTTAGGGTGGTTGCATTTTTGGCCACTGTATCAGCTACACTTGTTATGTCACTCAACAAACAAACCAAAAGCTTTGTAGTTGCTACCATTGGTAGCACCCCAATTACAGTTCCTCTTACTGCCAAGTTTCAGCATACTCCAGCTTTTGTGTTAGTCCATAAAATCTCTCTAATTTAAACCTTTCTAACACTTCTGTATCTAAACATTTTCATTGAAAAATTAACTTAATAGTGATAATAATATTATTAGACATTTAATTTTCACCTTATGATTTAATTTGTGGTGAGAATACAGGTACTTTGTGGTAGCCAATGGAATTGTCAGTCTTCATAATTTGGTTATGATAGCAATGGATATATTAGGACTCAAATTTCATAACAAAGGGCTCCAACTTGCACTGATTGCAGTATTTGACACGGTACAAAATAAATGACACCTCTAATTGATCATTTAAGTTAAAATTACTAACATATGAGAACTGATTGATTATATTTTTTTGATTAATTGGGTGAATTAGATGGCTTTGGCTCTAGCATCAAGTGGAGATGGTGCAGCAACATTTATGTCAGAATTGGGAAGGAATGGTAATTCACATGCAAAATGGAATAAAATATGTGACAAATTTGAGTCCTATTGCAACAGAGGTGGTGGTGCCTTAATAGCTTCTTTCATTGGGCTCATTCTCCTACTCATTATTACAGTGATGTCCATCAACAAACTCCTCAAACTAAATCGCAATTAA

Protein sequence

>MS.gene47926.t1

MASGNEEKIESGFNVVPETKPKKDWILLSLRVVAFLATVSATLVMSLNKQTKSFVVATIGSTPITVPLTAKFQHTPAFVYFVVANGIVSLHNLVMIAMDILGLKFHNKGLQLALIAVFDTMALALASSGDGAATFMSELGRNGNSHAKWNKICDKFESYCNRGGGALIASFIGLILLLIITVMSINKLLKLNRN