AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53368


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53368.t1 MTR_8g019700 99.438 178 1 0 1 178 1 178 3.59e-125 351
MS.gene53368.t1 MTR_2g041190 91.573 178 15 0 1 178 1 178 3.10e-104 298
MS.gene53368.t1 MTR_1g069715 45.714 175 95 0 4 178 468 642 8.21e-47 162
MS.gene53368.t1 MTR_2g047875 43.429 175 99 0 4 178 3 177 2.33e-42 141
MS.gene53368.t1 MTR_7g097170 48.571 175 90 0 4 178 3 177 1.42e-38 131
MS.gene53368.t1 MTR_4g057565 28.824 170 119 2 4 172 7 175 9.41e-18 77.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53368.t1 AT2G06530 92.697 178 13 0 1 178 2 179 6.34e-103 295
MS.gene53368.t1 AT2G06530 94.304 158 9 0 21 178 1 158 1.13e-89 261
MS.gene53368.t1 AT1G03950 45.714 175 95 0 4 178 3 177 3.74e-52 166
MS.gene53368.t1 AT5G44560 42.286 175 101 0 4 178 3 177 7.70e-46 149
MS.gene53368.t1 AT5G44560 42.286 175 101 0 4 178 3 177 2.10e-45 149
MS.gene53368.t1 AT5G44560 43.506 154 87 0 25 178 4 157 2.78e-40 135
MS.gene53368.t1 AT5G44560 37.857 140 86 1 4 142 3 142 2.57e-27 100
MS.gene53368.t1 AT5G22950 26.846 149 107 2 4 151 7 154 4.05e-15 70.9
MS.gene53368.t1 AT1G17730 24.667 150 113 0 27 176 19 168 1.26e-11 60.8

Find 32 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGTGTTGCTTTGAGAATTC+AGG 0.273393 8.4:-73415989 MS.gene53368:intron
CAAGCTTAAATCACAACTTC+AGG 0.310848 8.4:-73416010 MS.gene53368:CDS
AAATACCATTTGTCCTTGTT+TGG 0.313748 8.4:+73416752 None:intergenic
TTAGGGTGCTGTGAGGGTTA+TGG 0.358019 8.4:-73416076 MS.gene53368:intron
GGAACTTGTTTCTGAGGTAA+TGG 0.405606 8.4:-73415376 MS.gene53368:CDS
GAACTTGTTTCTGAGGTAAT+GGG 0.408793 8.4:-73415375 MS.gene53368:CDS
AAGCAATGGGTCAAATGAAT+AGG 0.409703 8.4:-73415461 MS.gene53368:CDS
TGCATTATCTTCTGTAAAGA+AGG 0.410358 8.4:+73415426 None:intergenic
GCAATTGATGATGCTTTAGA+AGG 0.423361 8.4:-73415348 MS.gene53368:CDS
CTGAAATCTACTCAAGCAAT+GGG 0.427818 8.4:-73415507 MS.gene53368:CDS
GAATGCCAAACAAGGACAAA+TGG 0.480489 8.4:-73416757 MS.gene53368:intron
AAAGGAGTTACTAAAGCAAT+GGG 0.498174 8.4:-73415474 MS.gene53368:CDS
AGTCTATTAGAGAAATTGAA+AGG 0.508512 8.4:-73416833 MS.gene53368:CDS
TGAAAGGCAGAATGAGAAGA+TGG 0.511267 8.4:-73415397 MS.gene53368:CDS
AGATAATGCAAGAGTTTGAA+AGG 0.512294 8.4:-73415413 MS.gene53368:CDS
AAGCTTAAATCACAACTTCA+GGG 0.512790 8.4:-73416009 MS.gene53368:CDS
GAAAGGAGTTACTAAAGCAA+TGG 0.516989 8.4:-73415475 MS.gene53368:CDS
TTTAGAAGGTGATGAAGAAG+AGG 0.518323 8.4:-73415334 MS.gene53368:CDS
TGCTGTGAGGGTTATGGCCA+AGG 0.523944 8.4:-73416070 MS.gene53368:CDS
GCGAGTTCTGATAAGATCCT+TGG 0.525743 8.4:+73416053 None:intergenic
GTCTATTAGAGAAATTGAAA+GGG 0.532893 8.4:-73416832 MS.gene53368:CDS
AATTTGTTAGGGTGCTGTGA+GGG 0.568995 8.4:-73416082 MS.gene53368:intron
AATCAAGTTCTTGATGAGAT+CGG 0.569717 8.4:-73415291 MS.gene53368:CDS
GAAGATGGAACTTGTTTCTG+AGG 0.576457 8.4:-73415382 MS.gene53368:CDS
CAATTTGTTAGGGTGCTGTG+AGG 0.590012 8.4:-73416083 MS.gene53368:intron
ACTGAAATCTACTCAAGCAA+TGG 0.611599 8.4:-73415508 MS.gene53368:CDS
GAAATCTACTCAAGCAATGG+GGG 0.624835 8.4:-73415505 MS.gene53368:CDS
ATTAAGAAGAATGCCAAACA+AGG 0.625704 8.4:-73416765 MS.gene53368:CDS
GAAATTGAAAGGGAAAGACA+AGG 0.633295 8.4:-73416822 MS.gene53368:CDS
AAATTGAAAGGGAAAGACAA+GGG 0.643400 8.4:-73416821 MS.gene53368:CDS
CGGAATCGATATCAACTCTG+AGG 0.660467 8.4:-73415271 None:intergenic
TGAAATCTACTCAAGCAATG+GGG 0.673116 8.4:-73415506 MS.gene53368:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAACAATAATTTAATAC+TGG - chr8.4:73415392-73415411 MS.gene53368:CDS 10.0%
!! AAAAACAATAATTTAATACT+GGG - chr8.4:73415393-73415412 MS.gene53368:CDS 10.0%
!!! AAAATTGATAATTACTTTTA+GGG - chr8.4:73416113-73416132 MS.gene53368:intron 10.0%
!!! ATTTTCACTTTTAATCAAAT+TGG - chr8.4:73415321-73415340 MS.gene53368:CDS 15.0%
!!! CAAAATTGATAATTACTTTT+AGG - chr8.4:73416112-73416131 MS.gene53368:intron 15.0%
!! ACATTCAAATTAAACCAAAA+CGG + chr8.4:73415996-73416015 None:intergenic 20.0%
!! CTAAAAACAAAAAACACTAA+TGG - chr8.4:73415930-73415949 MS.gene53368:intron 20.0%
!! TAAAAATTGTGACTTTGATT+AGG - chr8.4:73415443-73415462 MS.gene53368:CDS 20.0%
!!! AATTGAGTTTTTTGGTTAAT+GGG - chr8.4:73415466-73415485 MS.gene53368:CDS 20.0%
!!! AGTTTTGTTTCAATTTGTTA+GGG - chr8.4:73416293-73416312 MS.gene53368:intron 20.0%
!!! TGTTTTAGTTTCATAGTTAA+CGG - chr8.4:73416013-73416032 MS.gene53368:CDS 20.0%
!!! TTAGTTGTATATTTTGTTGT+AGG - chr8.4:73415711-73415730 MS.gene53368:intron 20.0%
! AAAATTTGAAGAATTACCTG+CGG + chr8.4:73415306-73415325 None:intergenic 25.0%
! AAACAAAAAACACTAATGGT+AGG - chr8.4:73415934-73415953 MS.gene53368:intron 25.0%
! AACAAAAAACACTAATGGTA+GGG - chr8.4:73415935-73415954 MS.gene53368:intron 25.0%
! AACTAAGTGCAATAGAAAAA+TGG + chr8.4:73415697-73415716 None:intergenic 25.0%
! AGTCTATTAGAGAAATTGAA+AGG - chr8.4:73415553-73415572 MS.gene53368:intron 25.0%
! CAAAAAACACAATAATTGCA+GGG + chr8.4:73415754-73415773 None:intergenic 25.0%
! GTCTATTAGAGAAATTGAAA+GGG - chr8.4:73415554-73415573 MS.gene53368:intron 25.0%
!! AACGGTGTTAATTGTTATTA+GGG - chr8.4:73416031-73416050 MS.gene53368:CDS 25.0%
!! CAATAGTTTTTGAATTTGCA+AGG + chr8.4:73415814-73415833 None:intergenic 25.0%
!! TAACGGTGTTAATTGTTATT+AGG - chr8.4:73416030-73416049 MS.gene53368:CDS 25.0%
!! TGTAAAACTTTTCAATCTGA+TGG + chr8.4:73416358-73416377 None:intergenic 25.0%
!!! GAATTGAGTTTTTTGGTTAA+TGG - chr8.4:73415465-73415484 MS.gene53368:CDS 25.0%
!!! GAGTTTTGTTTCAATTTGTT+AGG - chr8.4:73416292-73416311 MS.gene53368:intron 25.0%
!!! TGAGAAGTTTATTGCTTTTA+AGG - chr8.4:73416193-73416212 MS.gene53368:intron 25.0%
!!! TGATTAGGAATTGAGTTTTT+TGG - chr8.4:73415458-73415477 MS.gene53368:CDS 25.0%
!!! TTTATTGCTTTTAAGGATTG+TGG - chr8.4:73416200-73416219 MS.gene53368:intron 25.0%
AAAGGAGTTACTAAAGCAAT+GGG - chr8.4:73416912-73416931 MS.gene53368:intron 30.0%
AAATACCATTTGTCCTTGTT+TGG + chr8.4:73415637-73415656 None:intergenic 30.0%
AAATTGAAAGGGAAAGACAA+GGG - chr8.4:73415565-73415584 MS.gene53368:intron 30.0%
AAATTTGAAGAATTACCTGC+GGG + chr8.4:73415305-73415324 None:intergenic 30.0%
AACTTATCTGCTAAAATCCT+AGG + chr8.4:73416486-73416505 None:intergenic 30.0%
AAGCTTAAATCACAACTTCA+GGG - chr8.4:73416377-73416396 MS.gene53368:intron 30.0%
AATCAAGTTCTTGATGAGAT+CGG - chr8.4:73417095-73417114 MS.gene53368:intron 30.0%
AGATAATGCAAGAGTTTGAA+AGG - chr8.4:73416973-73416992 MS.gene53368:intron 30.0%
ATTAAGAAGAATGCCAAACA+AGG - chr8.4:73415621-73415640 MS.gene53368:intron 30.0%
CCAAAAAACACAATAATTGC+AGG + chr8.4:73415755-73415774 None:intergenic 30.0%
CTCAAACAAAAGAACTTACA+CGG + chr8.4:73415785-73415804 None:intergenic 30.0%
GAACAGGTATGTAATATGTT+TGG - chr8.4:73416250-73416269 MS.gene53368:intron 30.0%
GGTATGTAATATGTTTGGTT+TGG - chr8.4:73416255-73416274 MS.gene53368:intron 30.0%
GTCTGTAACATTACTTAATC+AGG + chr8.4:73415880-73415899 None:intergenic 30.0%
TCAACACAATCATAATCACA+AGG + chr8.4:73415361-73415380 None:intergenic 30.0%
TGCATTATCTTCTGTAAAGA+AGG + chr8.4:73416963-73416982 None:intergenic 30.0%
TTTCATGCTCATTCATACTA+TGG - chr8.4:73416744-73416763 MS.gene53368:intron 30.0%
! GTAAGTAACTGTGTTGTTAA+GGG - chr8.4:73416061-73416080 MS.gene53368:CDS 30.0%
! TCGTTGTATTATATCCGTTT+TGG - chr8.4:73415979-73415998 MS.gene53368:intron 30.0%
! TGTAAGTAACTGTGTTGTTA+AGG - chr8.4:73416060-73416079 MS.gene53368:CDS 30.0%
! TTGTTAAACAGAGCTTTTGA+GGG - chr8.4:73415509-73415528 MS.gene53368:CDS 30.0%
! TTTGTTAAACAGAGCTTTTG+AGG - chr8.4:73415508-73415527 MS.gene53368:CDS 30.0%
!! TTTTGGTTAATGGGTAGTTT+TGG - chr8.4:73415475-73415494 MS.gene53368:CDS 30.0%
!!! CCTGCAATTATTGTGTTTTT+TGG - chr8.4:73415752-73415771 MS.gene53368:intron 30.0%
!!! TGTTTTAACTACGTCATATG+CGG - chr8.4:73415832-73415851 MS.gene53368:intron 30.0%
AAGCAATGGGTCAAATGAAT+AGG - chr8.4:73416925-73416944 MS.gene53368:intron 35.0%
ACTGAAATCTACTCAAGCAA+TGG - chr8.4:73416878-73416897 MS.gene53368:intron 35.0%
CAAGCTTAAATCACAACTTC+AGG - chr8.4:73416376-73416395 MS.gene53368:intron 35.0%
CAGTGTCTACAATTAATCTC+AGG + chr8.4:73416862-73416881 None:intergenic 35.0%
CATTACTTAATCAGGCTACA+TGG + chr8.4:73415872-73415891 None:intergenic 35.0%
CCACTAGTAATGAAAGAAGA+AGG + chr8.4:73415669-73415688 None:intergenic 35.0%
CCTTCTTCTTTCATTACTAG+TGG - chr8.4:73415666-73415685 MS.gene53368:intron 35.0%
CTGAAATCTACTCAAGCAAT+GGG - chr8.4:73416879-73416898 MS.gene53368:intron 35.0%
GAAAGGAGTTACTAAAGCAA+TGG - chr8.4:73416911-73416930 MS.gene53368:intron 35.0%
GAAATTGAAAGGGAAAGACA+AGG - chr8.4:73415564-73415583 MS.gene53368:intron 35.0%
GCTACATGGCGTATTATATT+GGG + chr8.4:73415858-73415877 None:intergenic 35.0%
TGAAATCTACTCAAGCAATG+GGG - chr8.4:73416880-73416899 MS.gene53368:intron 35.0%
TGTCTACAATTAATCTCAGG+AGG + chr8.4:73416859-73416878 None:intergenic 35.0%
TTTAGAAGGTGATGAAGAAG+AGG - chr8.4:73417052-73417071 MS.gene53368:intron 35.0%
! TGAGCATGAAAGTATACACA+AGG + chr8.4:73416735-73416754 None:intergenic 35.0%
!! AGCTTTTGAGGGAGAATAAA+AGG - chr8.4:73415520-73415539 MS.gene53368:intron 35.0%
!! GAACTTGTTTCTGAGGTAAT+GGG - chr8.4:73417011-73417030 MS.gene53368:intron 35.0%
!! GCAATTGATGATGCTTTAGA+AGG - chr8.4:73417038-73417057 MS.gene53368:intron 35.0%
!! GGATTTTAGCAGATAAGTTG+AGG - chr8.4:73416487-73416506 MS.gene53368:intron 35.0%
!! TAGCGTGTTGTTATTTGAAC+AGG - chr8.4:73416234-73416253 MS.gene53368:intron 35.0%
CTCGTTATGTATCTGCAATG+AGG + chr8.4:73416712-73416731 None:intergenic 40.0%
GAAATCTACTCAAGCAATGG+GGG - chr8.4:73416881-73416900 MS.gene53368:intron 40.0%
GAATGCCAAACAAGGACAAA+TGG - chr8.4:73415629-73415648 MS.gene53368:intron 40.0%
GGCTACATGGCGTATTATAT+TGG + chr8.4:73415859-73415878 None:intergenic 40.0%
TGAAAGGCAGAATGAGAAGA+TGG - chr8.4:73416989-73417008 MS.gene53368:intron 40.0%
TTGAAGCTTATTGTGCTCCT+AGG - chr8.4:73416466-73416485 MS.gene53368:intron 40.0%
!! AATTTGTTAGGGTGCTGTGA+GGG - chr8.4:73416304-73416323 MS.gene53368:intron 40.0%
!! GAAGATGGAACTTGTTTCTG+AGG - chr8.4:73417004-73417023 MS.gene53368:intron 40.0%
!! GGAACTTGTTTCTGAGGTAA+TGG - chr8.4:73417010-73417029 MS.gene53368:intron 40.0%
GACAAGTATCCGACATGTGT+GGG - chr8.4:73416581-73416600 MS.gene53368:intron 45.0%
TGACAAGTATCCGACATGTG+TGG - chr8.4:73416580-73416599 MS.gene53368:intron 45.0%
! GCGAGTTCTGATAAGATCCT+TGG + chr8.4:73416336-73416355 None:intergenic 45.0%
!! CAATTTGTTAGGGTGCTGTG+AGG - chr8.4:73416303-73416322 MS.gene53368:intron 45.0%
!! GGGTGTTGCTTTGAGAATTC+AGG - chr8.4:73416397-73416416 MS.gene53368:intron 45.0%
!! TTAAAAATTATAAATTTAAC+AGG + chr8.4:73416796-73416815 None:intergenic 5.0%
ACCGACAGAGACATGGATAC+CGG + chr8.4:73416688-73416707 None:intergenic 50.0%
ATCCGACATGTGTGGGCATT+GGG - chr8.4:73416588-73416607 MS.gene53368:intron 50.0%
TATCCGACATGTGTGGGCAT+TGG - chr8.4:73416587-73416606 MS.gene53368:intron 50.0%
TCCGGTATCCATGTCTCTGT+CGG - chr8.4:73416684-73416703 MS.gene53368:intron 50.0%
! CGATGTGTAAGTGTCGTGTC+CGG - chr8.4:73416666-73416685 MS.gene53368:intron 50.0%
!! TTAGGGTGCTGTGAGGGTTA+TGG - chr8.4:73416310-73416329 MS.gene53368:intron 50.0%
GCAATGGGGGACGCTATGAA+AGG - chr8.4:73416894-73416913 MS.gene53368:intron 55.0%
GGCAGTAGAAAAACTCCCGC+AGG - chr8.4:73415287-73415306 MS.gene53368:CDS 55.0%
TGCCCAATGCCCACACATGT+CGG + chr8.4:73416593-73416612 None:intergenic 55.0%
TGCTGTGAGGGTTATGGCCA+AGG - chr8.4:73416316-73416335 MS.gene53368:intron 55.0%
!! ATGAGGCACCGACAGAGACA+TGG + chr8.4:73416695-73416714 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 73415272 73417136 73415272 ID=MS.gene53368
chr8.4 mRNA 73415272 73417136 73415272 ID=MS.gene53368.t1;Parent=MS.gene53368
chr8.4 exon 73417100 73417136 73417100 ID=MS.gene53368.t1.exon1;Parent=MS.gene53368.t1
chr8.4 CDS 73417100 73417136 73417100 ID=cds.MS.gene53368.t1;Parent=MS.gene53368.t1
chr8.4 exon 73416758 73416888 73416758 ID=MS.gene53368.t1.exon2;Parent=MS.gene53368.t1
chr8.4 CDS 73416758 73416888 73416758 ID=cds.MS.gene53368.t1;Parent=MS.gene53368.t1
chr8.4 exon 73415990 73416094 73415990 ID=MS.gene53368.t1.exon3;Parent=MS.gene53368.t1
chr8.4 CDS 73415990 73416094 73415990 ID=cds.MS.gene53368.t1;Parent=MS.gene53368.t1
chr8.4 exon 73415272 73415532 73415272 ID=MS.gene53368.t1.exon4;Parent=MS.gene53368.t1
chr8.4 CDS 73415272 73415532 73415272 ID=cds.MS.gene53368.t1;Parent=MS.gene53368.t1
Gene Sequence

>MS.gene53368

ATGAGTTTTCTGTTCGGCAGTAGAAAAACTCCCGCAGGTAATTCTTCAAATTTTCACTTTTAATCAAATTGGTTTTTTTATTTTCTCCTTGTGATTATGATTGTGTTGATTGAATTGTGAAAAAAACAATAATTTAATACTGGGTTGTTTCTTATTTGCTTTTCTAGTTTGTAAAAATTGTGACTTTGATTAGGAATTGAGTTTTTTGGTTAATGGGTAGTTTTGGTTTTTGTGATTTTGTTAAACAGAGCTTTTGAGGGAGAATAAAAGGATGCTTGATAAGTCTATTAGAGAAATTGAAAGGGAAAGACAAGGGTTGCAGTCTCAAGAAAAGAAATTGATTGTAGAGATTAAGAAGAATGCCAAACAAGGACAAATGGTATTTTCTATTATGCCTTCTTCTTTCATTACTAGTGGATTATCCATTTTTCTATTGCACTTAGTTGTATATTTTGTTGTAGGTTGAATTATGTTTTATTCCCTGCAATTATTGTGTTTTTTGGTTTTAATCCGTGTAAGTTCTTTTGTTTGAGTTCAGTCCTTGCAAATTCAAAAACTATTGTTTTAACTACGTCATATGCGGCCCAATATAATACGCCATGTAGCCTGATTAAGTAATGTTACAGACTAAAAGCAGTTGTTTTTTAATTTGCACAGACTAAAAACAAAAAACACTAATGGTAGGGACTAAAGTTGTATTTAAACTTTCGTTGTATTATATCCGTTTTGGTTTAATTTGAATGTTTTAGTTTCATAGTTAACGGTGTTAATTGTTATTAGGGCAAATTTGTAAGTAACTGTGTTGTTAAGGGTAAATTTGTAAAGAAAAAAATGTCTGAACAAAATTGATAATTACTTTTAGGGTTATAGTATAGTGTACAAGTATAGATTATTTAGAATTCTTAAGTAGTCTATGTCTGATGAGAAGTTTATTGCTTTTAAGGATTGTGGTTTTAATATAGTAGCGTGTTGTTATTTGAACAGGTATGTAATATGTTTGGTTTGGTTTGAGTAGTTTCTGAGTTTTGTTTCAATTTGTTAGGGTGCTGTGAGGGTTATGGCCAAGGATCTTATCAGAACTCGCCATCAGATTGAAAAGTTTTACAAGCTTAAATCACAACTTCAGGGTGTTGCTTTGAGAATTCAGGTTTGTTTTGCATAGTATGTTGTGTTTTCTTTGTCTGTAGCTCAACTTTGAAGCTTATTGTGCTCCTAGGATTTTAGCAGATAAGTTGAGGTTATGTGTATAGTGCTGTACTACTGTGATGCATACATATTATGTAGCACTGACACTTCTGATCAAAGACGTGACAAGTATCCGACATGTGTGGGCATTGGGCATTGACATACGTGATTACATTCAATTAATTCATTTTCTTTAATTATTGTCGTTTCGATGTGTAAGTGTCGTGTCCGGTATCCATGTCTCTGTCGGTGCCTCATTGCAGATACATAACGAGCCTTGTGTATACTTTCATGCTCATTCATACTATGGCGATATCATATACATACTGCAAGAATCCTGTTAAATTTATAATTTTTAATCTGTAAGATTGATTGATTTATTATAGCTTTTAAAAATGACCTCCTGAGATTAATTGTAGACACTGAAATCTACTCAAGCAATGGGGGACGCTATGAAAGGAGTTACTAAAGCAATGGGTCAAATGAATAGGCAGATGAATTTGCCTTCTTTACAGAAGATAATGCAAGAGTTTGAAAGGCAGAATGAGAAGATGGAACTTGTTTCTGAGGTAATGGGAGATGCAATTGATGATGCTTTAGAAGGTGATGAAGAAGAGGAAGAGACAGAAGATCTTGTGAATCAAGTTCTTGATGAGATCGGAATCGATATCAACTCTGAG

Protein sequence

>MS.gene53368.t1

MSFLFGSRKTPAELLRENKRMLDKSIREIERERQGLQSQEKKLIVEIKKNAKQGQMGAVRVMAKDLIRTRHQIEKFYKLKSQLQGVALRIQTLKSTQAMGDAMKGVTKAMGQMNRQMNLPSLQKIMQEFERQNEKMELVSEVMGDAIDDALEGDEEEEETEDLVNQVLDEIGIDINSE