AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54777

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54777.t1 MTR_1g095540 96.725 458 15 0 1 458 1 458 0.0 931
MS.gene54777.t1 MTR_1g095560 85.808 458 65 0 1 458 1 458 0.0 825
MS.gene54777.t1 MTR_1g095560 80.408 490 64 2 1 458 1 490 0.0 810
MS.gene54777.t1 MTR_2g062990 53.829 457 205 5 5 457 9 463 0.0 517
MS.gene54777.t1 MTR_2g063000 52.391 460 213 4 2 457 7 464 1.06e-178 508
MS.gene54777.t1 MTR_7g013790 53.913 460 197 8 8 457 11 465 3.47e-176 502
MS.gene54777.t1 MTR_2g062980 52.079 457 213 5 5 457 9 463 1.84e-175 500
MS.gene54777.t1 MTR_2g081620 52.212 452 209 6 8 455 14 462 1.24e-173 496
MS.gene54777.t1 MTR_4g075150 50.110 455 216 8 9 456 11 461 1.77e-155 449
MS.gene54777.t1 MTR_4g075150 50.109 457 215 8 9 456 11 463 6.06e-155 448
MS.gene54777.t1 MTR_2g063000 48.043 460 202 5 2 457 7 433 1.06e-154 446
MS.gene54777.t1 MTR_3g079560 52.668 431 191 7 29 458 74 492 1.18e-154 448
MS.gene54777.t1 MTR_3g079570 52.184 435 194 7 26 458 70 492 1.37e-152 443
MS.gene54777.t1 MTR_3g079590 51.991 427 192 7 26 450 69 484 1.27e-149 435
MS.gene54777.t1 MTR_3g034290 48.372 430 212 5 28 456 70 490 5.62e-149 434
MS.gene54777.t1 MTR_3g079600 52.113 426 190 6 28 450 71 485 1.48e-148 432
MS.gene54777.t1 MTR_3g079620 48.472 458 213 8 12 458 55 500 6.44e-147 429
MS.gene54777.t1 MTR_1g021010 49.312 436 204 7 17 450 60 480 4.33e-144 421
MS.gene54777.t1 MTR_4g122950 45.263 475 236 6 5 458 8 479 1.47e-142 417
MS.gene54777.t1 MTR_4g122960 45.243 473 237 7 5 458 8 477 1.67e-141 414
MS.gene54777.t1 MTR_3g079630 49.772 438 193 9 26 450 69 492 4.68e-141 414
MS.gene54777.t1 MTR_1g020090 48.513 437 203 7 19 450 72 491 1.43e-140 412
MS.gene54777.t1 MTR_4g089155 47.273 440 216 6 31 458 52 487 5.13e-139 408
MS.gene54777.t1 MTR_1g021020 47.826 437 208 7 17 450 62 481 2.88e-137 404
MS.gene54777.t1 MTR_0003s0560 47.113 433 213 9 28 457 69 488 3.86e-137 403
MS.gene54777.t1 MTR_2g012860 44.050 479 235 8 3 457 5 474 3.55e-136 400
MS.gene54777.t1 MTR_2g012850 44.136 469 243 8 3 458 5 467 1.06e-132 391
MS.gene54777.t1 MTR_3g034290 48.851 348 169 4 28 375 70 408 1.40e-119 355
MS.gene54777.t1 MTR_2g449850 41.535 443 235 9 28 458 34 464 7.88e-113 340
MS.gene54777.t1 MTR_3g434850 40.938 469 250 12 1 457 2 455 2.35e-111 336
MS.gene54777.t1 MTR_8g099930 39.872 469 247 13 12 458 9 464 6.84e-110 333
MS.gene54777.t1 MTR_3g498755 41.043 441 237 12 31 456 13 445 8.55e-110 332
MS.gene54777.t1 MTR_7g021130 41.136 440 234 13 31 456 29 457 3.21e-105 320
MS.gene54777.t1 MTR_3g090130 41.324 438 243 9 30 458 26 458 5.00e-105 320
MS.gene54777.t1 MTR_5g072570 39.440 464 259 10 11 458 6 463 1.88e-104 319
MS.gene54777.t1 MTR_3g090150 39.041 438 255 8 30 458 31 465 8.67e-100 307
MS.gene54777.t1 MTR_1g040370 37.797 463 229 14 2 456 4 415 3.34e-96 296
MS.gene54777.t1 MTR_0003s0560 48.134 268 133 5 28 294 69 331 8.77e-82 256
MS.gene54777.t1 MTR_8g011350 31.727 498 280 14 2 450 4 490 4.36e-74 241
MS.gene54777.t1 MTR_7g051430 32.800 500 276 14 1 450 1 490 5.91e-72 236
MS.gene54777.t1 MTR_7g080180 32.777 479 258 14 33 455 28 498 5.90e-70 230
MS.gene54777.t1 MTR_1g102130 32.563 476 252 15 6 456 59 490 1.43e-69 229
MS.gene54777.t1 MTR_8g011350 30.672 476 270 14 2 428 4 468 4.14e-64 214
MS.gene54777.t1 MTR_7g080180 31.579 437 236 13 33 414 28 456 1.67e-56 194
MS.gene54777.t1 MTR_2g018600 29.241 448 280 15 33 455 34 469 1.63e-53 186
MS.gene54777.t1 MTR_2g018590 28.481 474 300 15 5 455 12 469 2.08e-52 183
MS.gene54777.t1 MTR_7g051430 31.840 424 230 13 1 375 1 414 8.67e-52 180
MS.gene54777.t1 MTR_4g021500 31.151 443 276 14 32 455 39 471 3.53e-51 186
MS.gene54777.t1 MTR_4g021500 28.947 418 267 13 45 452 487 884 2.09e-44 166
MS.gene54777.t1 MTR_4g021500 31.151 443 276 14 32 455 39 471 4.60e-51 186
MS.gene54777.t1 MTR_4g021500 28.935 432 267 14 45 452 487 902 7.73e-45 168
MS.gene54777.t1 MTR_4g021520 29.613 439 281 12 32 452 39 467 5.60e-51 179
MS.gene54777.t1 MTR_4g021535 28.387 465 304 13 6 452 10 463 6.49e-51 179
MS.gene54777.t1 MTR_3g100420 29.854 479 292 16 5 452 28 493 1.27e-50 179
MS.gene54777.t1 MTR_4g021490 29.157 439 283 13 32 452 39 467 3.25e-50 177
MS.gene54777.t1 MTR_3g045770 28.384 458 292 12 7 447 8 446 1.78e-49 175
MS.gene54777.t1 MTR_4g021530 28.604 444 289 11 27 452 34 467 1.88e-49 175
MS.gene54777.t1 MTR_4g021510 29.139 453 265 17 32 452 38 466 2.56e-49 175
MS.gene54777.t1 MTR_2g018630 27.742 465 291 16 13 455 24 465 2.18e-45 164
MS.gene54777.t1 MTR_0075s0010 31.948 385 179 16 30 402 5 318 5.78e-42 152
MS.gene54777.t1 MTR_4g021520 29.500 400 255 11 32 414 39 428 7.73e-42 154
MS.gene54777.t1 MTR_2g095730 26.841 421 275 12 47 453 94 495 2.34e-40 151
MS.gene54777.t1 MTR_1g050545 28.777 417 261 15 45 447 81 475 6.95e-40 150
MS.gene54777.t1 MTR_4g057585 27.064 436 263 12 38 450 87 490 7.92e-40 150
MS.gene54777.t1 MTR_5g097180 27.885 416 270 11 49 455 43 437 2.13e-39 148
MS.gene54777.t1 MTR_2g095750 26.056 426 269 12 47 453 100 498 1.08e-38 147
MS.gene54777.t1 MTR_4g057595 26.636 428 259 12 47 451 97 492 1.48e-38 146
MS.gene54777.t1 MTR_4g084710 28.605 423 258 15 49 455 45 439 1.03e-37 143
MS.gene54777.t1 MTR_2g009200 27.119 472 279 15 6 450 1 434 1.27e-36 140
MS.gene54777.t1 MTR_4g057600 26.714 423 269 15 47 453 95 492 2.40e-36 140
MS.gene54777.t1 MTR_4g057570 24.890 454 272 14 32 453 74 490 4.19e-36 139
MS.gene54777.t1 MTR_1g094155 26.781 407 273 11 49 447 92 481 4.97e-36 139
MS.gene54777.t1 MTR_4g021535 25.806 465 269 16 6 452 10 416 1.24e-34 134
MS.gene54777.t1 MTR_2g018630 26.462 359 234 12 112 453 13 358 1.75e-29 118
MS.gene54777.t1 MTR_2g095710 25.962 416 244 13 62 453 106 481 2.53e-29 120
MS.gene54777.t1 MTR_1g051175 41.085 129 64 5 27 150 2 123 8.87e-19 86.7
MS.gene54777.t1 MTR_4g056520 22.717 449 290 16 41 452 27 455 5.79e-16 80.1
MS.gene54777.t1 MTR_7g100560 26.852 216 89 6 42 257 67 213 1.78e-13 71.6
MS.gene54777.t1 MTR_4g122970 39.759 83 40 2 8 82 7 87 1.88e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54777.t1 AT3G07990 73.563 435 115 0 22 456 23 457 0.0 681
MS.gene54777.t1 AT2G35780 68.421 437 132 3 22 456 19 451 0.0 636
MS.gene54777.t1 AT4G30610 55.224 469 194 7 1 458 1 464 0.0 533
MS.gene54777.t1 AT2G24000 57.047 447 179 6 22 458 20 463 0.0 521
MS.gene54777.t1 AT2G24010 55.402 435 189 4 25 458 23 453 3.82e-176 501
MS.gene54777.t1 AT2G35770 55.022 458 192 7 5 458 13 460 2.92e-175 499
MS.gene54777.t1 AT3G02110 54.911 448 182 7 26 458 31 473 2.24e-174 498
MS.gene54777.t1 AT4G30810 54.292 431 192 2 23 450 28 456 2.58e-173 495
MS.gene54777.t1 AT2G24010 53.333 450 190 5 25 458 23 468 6.46e-171 488
MS.gene54777.t1 AT2G24010 50.749 467 185 6 33 458 2 464 2.66e-162 466
MS.gene54777.t1 AT5G23210 50.218 458 198 8 25 457 44 496 6.84e-153 444
MS.gene54777.t1 AT3G63470 47.597 437 209 8 28 458 77 499 3.48e-146 427
MS.gene54777.t1 AT3G17180 47.778 450 216 8 25 458 31 477 3.52e-139 408
MS.gene54777.t1 AT1G61130 46.137 466 238 8 1 458 2 462 3.52e-138 405
MS.gene54777.t1 AT5G08260 43.305 478 238 11 5 457 8 477 7.14e-137 402
MS.gene54777.t1 AT5G23210 49.395 413 179 8 25 412 44 451 3.07e-133 392
MS.gene54777.t1 AT1G11080 46.916 454 218 7 24 458 42 491 2.02e-132 391
MS.gene54777.t1 AT1G11080 46.916 454 218 7 24 458 42 491 2.33e-132 391
MS.gene54777.t1 AT2G24010 53.203 359 148 5 33 375 2 356 1.92e-129 379
MS.gene54777.t1 AT3G52020 46.101 436 220 11 26 456 73 498 3.42e-129 383
MS.gene54777.t1 AT4G15100 43.867 481 242 9 2 458 7 483 5.58e-129 382
MS.gene54777.t1 AT5G23210 48.635 403 180 6 80 457 1 401 1.47e-127 376
MS.gene54777.t1 AT5G23210 48.861 395 175 6 88 457 8 400 2.37e-126 373
MS.gene54777.t1 AT3G17180 45.477 409 205 7 25 418 31 436 5.65e-120 357
MS.gene54777.t1 AT2G05850 42.282 447 241 9 19 458 50 486 2.32e-119 358
MS.gene54777.t1 AT3G52000 42.793 444 238 12 19 458 50 481 2.36e-115 347
MS.gene54777.t1 AT3G52010 41.031 446 243 11 21 458 53 486 6.25e-114 344
MS.gene54777.t1 AT2G33530 43.363 452 227 13 22 456 23 462 4.65e-112 338
MS.gene54777.t1 AT5G23210 47.714 350 156 6 88 412 8 355 1.29e-106 321
MS.gene54777.t1 AT2G24000 42.282 447 182 9 22 458 20 400 4.66e-106 321
MS.gene54777.t1 AT1G28110 41.043 441 233 11 31 456 30 458 5.48e-106 323
MS.gene54777.t1 AT1G28110 41.043 441 233 11 31 456 30 458 5.48e-106 323
MS.gene54777.t1 AT2G12480 39.310 435 254 6 29 457 28 458 2.22e-105 321
MS.gene54777.t1 AT5G42240 40.498 442 247 8 29 458 29 466 2.98e-104 318
MS.gene54777.t1 AT1G43780 40.045 442 244 9 30 456 35 470 5.67e-104 318
MS.gene54777.t1 AT2G12480 38.249 434 236 7 29 457 28 434 8.57e-99 303
MS.gene54777.t1 AT2G12480 38.018 434 235 7 29 457 28 432 1.06e-97 301
MS.gene54777.t1 AT2G12480 37.931 435 235 8 29 457 28 433 3.67e-96 296
MS.gene54777.t1 AT5G42230 37.415 441 260 8 29 457 25 461 5.33e-95 295
MS.gene54777.t1 AT5G42230 37.415 441 260 8 29 457 25 461 5.33e-95 295
MS.gene54777.t1 AT4G12910 34.082 490 269 12 14 455 14 497 3.11e-80 257
MS.gene54777.t1 AT3G25420 32.860 493 273 13 14 455 9 494 1.64e-75 245
MS.gene54777.t1 AT3G56540 61.667 180 65 3 10 189 57 232 3.12e-72 229
MS.gene54777.t1 AT4G12910 33.036 448 247 11 14 414 14 455 9.17e-67 221
MS.gene54777.t1 AT2G22990 31.090 431 265 13 33 453 23 431 5.62e-59 200
MS.gene54777.t1 AT2G22970 31.178 433 264 14 33 455 25 433 3.41e-57 195
MS.gene54777.t1 AT2G22920 30.485 433 269 13 33 455 25 435 3.73e-57 195
MS.gene54777.t1 AT2G22990 30.882 408 251 12 33 430 23 409 3.45e-53 185
MS.gene54777.t1 AT2G22990 30.958 407 250 12 33 429 23 408 4.70e-53 184
MS.gene54777.t1 AT1G73270 31.034 435 262 13 33 455 33 441 1.60e-52 183
MS.gene54777.t1 AT1G73270 31.034 435 262 13 33 455 33 441 2.48e-52 182
MS.gene54777.t1 AT1G73280 30.296 439 260 14 33 455 33 441 8.93e-52 181
MS.gene54777.t1 AT2G23000 28.538 431 278 13 33 453 25 435 1.08e-51 181
MS.gene54777.t1 AT2G23000 28.538 431 278 13 33 453 25 435 1.08e-51 181
MS.gene54777.t1 AT1G73300 30.394 431 270 12 33 455 33 441 1.55e-51 180
MS.gene54777.t1 AT1G73300 30.394 431 270 12 33 455 33 441 1.55e-51 180
MS.gene54777.t1 AT2G22990 29.792 433 263 14 33 455 23 424 1.65e-51 180
MS.gene54777.t1 AT2G22970 30.383 418 258 13 33 440 25 419 7.22e-51 179
MS.gene54777.t1 AT2G23010 29.176 449 288 13 15 453 7 435 1.00e-50 178
MS.gene54777.t1 AT1G73290 29.358 436 266 12 33 455 32 438 2.21e-50 177
MS.gene54777.t1 AT2G22970 30.637 408 250 13 33 430 25 409 2.73e-50 176
MS.gene54777.t1 AT2G23010 28.953 449 289 13 15 453 7 435 3.34e-50 177
MS.gene54777.t1 AT2G22980 29.206 428 265 12 33 450 26 425 4.14e-49 174
MS.gene54777.t1 AT2G22920 30.357 392 243 11 33 415 25 395 2.09e-48 171
MS.gene54777.t1 AT3G10450 28.538 431 278 12 33 455 29 437 6.17e-48 171
MS.gene54777.t1 AT3G12203 28.704 432 275 11 33 455 9 416 8.18e-48 170
MS.gene54777.t1 AT3G12203 28.704 432 275 11 33 455 30 437 1.34e-47 170
MS.gene54777.t1 AT5G36180 29.837 429 271 12 33 453 33 439 2.78e-47 169
MS.gene54777.t1 AT3G12220 29.493 434 266 12 33 453 27 433 2.90e-47 169
MS.gene54777.t1 AT5G09640 27.310 487 296 13 1 453 1 463 4.09e-46 166
MS.gene54777.t1 AT1G33540 28.315 445 288 13 33 455 29 464 4.73e-46 166
MS.gene54777.t1 AT5G22980 28.873 426 259 14 45 453 96 494 1.49e-45 166
MS.gene54777.t1 AT3G12240 28.571 434 274 14 33 455 28 436 2.53e-45 164
MS.gene54777.t1 AT1G73310 28.440 436 268 13 33 453 33 439 3.54e-45 163
MS.gene54777.t1 AT3G12230 28.802 434 273 13 33 455 27 435 6.39e-45 162
MS.gene54777.t1 AT3G10450 28.224 411 265 12 51 453 4 392 1.49e-44 160
MS.gene54777.t1 AT2G22980 28.230 418 263 11 33 440 26 416 6.51e-44 160
MS.gene54777.t1 AT2G22980 28.676 408 254 11 33 430 26 406 1.72e-43 158
MS.gene54777.t1 AT1G33540 28.103 427 276 13 51 455 4 421 2.68e-43 157
MS.gene54777.t1 AT3G12220 28.846 416 256 12 51 453 15 403 5.78e-43 156
MS.gene54777.t1 AT1G73290 29.114 395 240 10 33 415 32 398 1.65e-42 155
MS.gene54777.t1 AT3G12203 28.429 401 254 11 62 453 1 377 4.13e-42 154
MS.gene54777.t1 AT3G10410 27.506 429 273 12 38 452 90 494 1.72e-41 155
MS.gene54777.t1 AT3G10450 28.079 406 263 11 33 430 29 413 4.48e-41 152
MS.gene54777.t1 AT3G45010 26.932 427 282 11 38 453 92 499 5.37e-41 153
MS.gene54777.t1 AT2G22990 30.655 336 209 10 123 453 1 317 9.49e-41 148
MS.gene54777.t1 AT2G22990 30.357 336 210 10 123 453 1 317 3.52e-40 147
MS.gene54777.t1 AT1G73310 28.537 410 249 12 33 427 33 413 5.25e-40 149
MS.gene54777.t1 AT2G22920 31.392 309 183 10 33 332 25 313 7.55e-38 140
MS.gene54777.t1 AT3G10450 27.577 388 252 11 51 430 4 370 8.16e-38 142
MS.gene54777.t1 AT2G22970 31.068 309 183 10 33 332 25 312 1.02e-36 137
MS.gene54777.t1 AT1G73280 33.086 269 162 7 33 287 33 297 1.41e-36 138
MS.gene54777.t1 AT1G73290 32.917 240 152 4 33 267 32 267 6.57e-36 135
MS.gene54777.t1 AT1G73280 27.762 353 217 11 112 453 12 337 2.57e-34 131
MS.gene54777.t1 AT1G73280 27.729 339 207 11 126 453 1 312 9.87e-32 124
MS.gene54777.t1 AT2G22980 29.102 323 192 10 33 344 26 322 6.61e-31 122
MS.gene54777.t1 AT1G15000 28.141 398 253 11 49 438 37 409 7.73e-31 124
MS.gene54777.t1 AT5G36180 27.536 345 228 9 112 453 12 337 4.65e-30 120
MS.gene54777.t1 AT5G36180 27.536 345 228 9 112 453 12 337 4.65e-30 120
MS.gene54777.t1 AT3G12220 33.945 218 133 5 33 244 27 239 4.50e-29 118
MS.gene54777.t1 AT2G22980 26.518 313 201 8 123 430 1 289 4.84e-25 104
MS.gene54777.t1 AT5G36180 26.708 322 215 8 112 430 12 315 8.15e-25 104
MS.gene54777.t1 AT5G36180 25.882 340 220 8 112 437 12 333 2.36e-23 100
MS.gene54777.t1 AT1G73310 25.846 325 207 8 112 427 12 311 3.93e-23 100
MS.gene54777.t1 AT5G22960 31.343 201 102 5 11 211 10 174 6.98e-23 96.3
MS.gene54777.t1 AT2G22920 26.838 272 176 9 189 455 3 256 3.18e-22 95.9
MS.gene54777.t1 AT2G27920 22.814 469 296 19 9 439 5 445 5.32e-17 83.6
MS.gene54777.t1 AT2G22960 30.597 134 88 4 321 453 53 182 3.98e-14 71.2

Find 105 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAAACATTGGCTGTGATTT+TGG 0.097316 1.2:+72897104 MS.gene54777:CDS
GATGATTATCATGATTATAT+TGG 0.232593 1.2:+72897026 MS.gene54777:CDS
AAACAACACAACACAGAATA+TGG 0.249064 1.2:-72895331 None:intergenic
TTAATGCCGGTCTCCGGATA+TGG 0.251810 1.2:+72897678 MS.gene54777:CDS
GTGGTACTGTTTGGGATTAT+TGG 0.264427 1.2:+72897612 MS.gene54777:intron
AATTGTTAACCAGAGAAATA+AGG 0.271531 1.2:+72896853 MS.gene54777:CDS
GTTGGATTTGAACAGTATTC+AGG 0.275404 1.2:+72895447 MS.gene54777:CDS
GCACTTCATGCCAATGTAAC+TGG 0.277277 1.2:+72897459 MS.gene54777:CDS
GGTTGTTCTTCCATAGCTTA+TGG 0.284743 1.2:+72895585 MS.gene54777:CDS
TAAAACTAGTGGCTTTGAAT+TGG 0.298079 1.2:-72895544 None:intergenic
AAATTGGTCCTCTTCGTATT+AGG 0.299191 1.2:+72895619 MS.gene54777:CDS
TTCTTGACAGTGGTACTGTT+TGG 0.300868 1.2:+72897603 MS.gene54777:intron
TGAAGTGCCTTCTGAACTTC+TGG 0.312729 1.2:-72897444 None:intergenic
AATCCCTTAAAATTTATAAC+TGG 0.318228 1.2:-72896887 None:intergenic
AATGCATCTGATTAACTTGA+TGG 0.320292 1.2:-72898583 None:intergenic
TCTTGACAGTGGTACTGTTT+GGG 0.320433 1.2:+72897604 MS.gene54777:intron
TATGGTGCTTCTGAAGAAAT+TGG 0.320537 1.2:+72895603 MS.gene54777:CDS
TTAATAAGTTCTTGATATAT+AGG 0.324336 1.2:-72897659 None:intergenic
GGATGGAGTCAAGTTTATAA+AGG 0.364164 1.2:+72898457 MS.gene54777:CDS
TACCGATTCCCTTATTTCTC+TGG 0.370613 1.2:-72896862 None:intergenic
TTACAATTGTTCCAAGCATA+AGG 0.383194 1.2:-72895669 None:intergenic
TGGTACCCTTGGTATGATAA+TGG 0.388411 1.2:+72898184 MS.gene54777:CDS
AAAACTAGTGGCTTTGAATT+GGG 0.395531 1.2:-72895543 None:intergenic
ACTGCAACTCGATATTCTAT+TGG 0.429971 1.2:+72898133 MS.gene54777:CDS
AATGTTTAGCTTCGTATATG+TGG 0.431212 1.2:-72897090 None:intergenic
CTCGAGTCTCCTGCTGGTGT+TGG 0.436875 1.2:+72895804 MS.gene54777:CDS
CATAGAGAATTCTACATGGC+TGG 0.440477 1.2:+72896696 MS.gene54777:CDS
ATAAGCTATGGAAGAACAAC+CGG 0.440804 1.2:-72895583 None:intergenic
ATTGTTAACCAGAGAAATAA+GGG 0.444696 1.2:+72896854 MS.gene54777:CDS
TTTACTTGAATCCTTATGCT+TGG 0.446628 1.2:+72895658 MS.gene54777:CDS
TCCATCGGTGCAATGTTTGC+AGG 0.447339 1.2:+72897139 MS.gene54777:CDS
ATATTGGAACATTTGAGTAC+TGG 0.448006 1.2:+72897042 MS.gene54777:CDS
GATGATGGTTGGTAGCTTCA+AGG 0.449314 1.2:-72898158 None:intergenic
GATGGAGTCAAGTTTATAAA+GGG 0.457624 1.2:+72898458 MS.gene54777:CDS
ACTGAGCAAGGAAACATTGA+TGG 0.464521 1.2:+72897179 MS.gene54777:CDS
CCATCATCAATTGGTACCCT+TGG 0.465365 1.2:+72898173 MS.gene54777:CDS
GATAAAATTAAGTGGTTACC+AGG 0.466526 1.2:+72895411 MS.gene54777:CDS
ACATTTGGTGACCAGAAAAC+AGG 0.467712 1.2:+72895861 MS.gene54777:CDS
TAACTGGGATTTCATATGCT+TGG 0.468731 1.2:+72897475 MS.gene54777:CDS
TCGTTGCAACAGGTTGGAAA+TGG 0.470766 1.2:+72896996 MS.gene54777:intron
ATATAGGAAGCATAGACAAT+GGG 0.471053 1.2:-72897643 None:intergenic
CTCACATTGGTCACAGTAAG+AGG 0.474837 1.2:+72898481 MS.gene54777:CDS
AACTTATTAATGCCGGTCTC+CGG 0.476370 1.2:+72897672 MS.gene54777:CDS
ACAACAGATATGCAGACATT+TGG 0.477904 1.2:+72895846 MS.gene54777:CDS
AACCAGAGAAATAAGGGAAT+CGG 0.478660 1.2:+72896860 MS.gene54777:CDS
CATATGCTTGGAAGGCATGC+AGG 0.479209 1.2:+72897487 MS.gene54777:CDS
GTCTCCGGATATGGGTATAC+AGG 0.479783 1.2:+72897687 MS.gene54777:CDS
TAATGCCGGTCTCCGGATAT+GGG 0.480535 1.2:+72897679 MS.gene54777:CDS
AAGTAAAGTGACTTGCCATC+AGG 0.482213 1.2:-72895642 None:intergenic
ATAGAGAATTCTACATGGCT+GGG 0.484144 1.2:+72896697 MS.gene54777:CDS
AGCTACCAACCATCATCAAT+TGG 0.484161 1.2:+72898164 MS.gene54777:CDS
TTCAGAAGCACCATAAGCTA+TGG 0.487990 1.2:-72895595 None:intergenic
TTATGGCTTAATGGTGGTCC+CGG 0.492550 1.2:+72895564 MS.gene54777:CDS
AGGTCATTATGTTCTTCAGT+TGG 0.497859 1.2:+72896826 MS.gene54777:intron
GCCTGCAAACATTGCACCGA+TGG 0.501680 1.2:-72897140 None:intergenic
CTGTTCCTCGAGTCTCCTGC+TGG 0.503070 1.2:+72895798 MS.gene54777:CDS
TAAGAAAAGCCAACACCAGC+AGG 0.507655 1.2:-72895813 None:intergenic
CACTTCATGCCAATGTAACT+GGG 0.519280 1.2:+72897460 MS.gene54777:CDS
TGGGATTTCATATGCTTGGA+AGG 0.530564 1.2:+72897479 MS.gene54777:CDS
AAAAGAGAGATAAAATTAAG+TGG 0.531113 1.2:+72895403 MS.gene54777:CDS
GGTACCTGTATACCCATATC+CGG 0.532682 1.2:-72897691 None:intergenic
TATCAAGAACTTATTAATGC+CGG 0.540455 1.2:+72897665 MS.gene54777:CDS
TTGGTCACAGTAAGAGGTGC+TGG 0.540636 1.2:+72898487 MS.gene54777:CDS
ACTGTTGCAATTACTGAGCA+AGG 0.541227 1.2:+72897167 MS.gene54777:CDS
TGGGTCCATTCAGCATCCAT+CGG 0.550429 1.2:+72897124 MS.gene54777:CDS
ATGGCTGGGGAAAGTTATGC+AGG 0.552095 1.2:+72896711 MS.gene54777:CDS
AGCGATGTGGTATCGTTACA+AGG 0.553440 1.2:-72897221 None:intergenic
GAATATCGAGTTGCAGTCAG+TGG 0.560041 1.2:-72898127 None:intergenic
TTGCACCGATGGATGCTGAA+TGG 0.561647 1.2:-72897129 None:intergenic
CCAAGGGTACCAATTGATGA+TGG 0.575493 1.2:-72898173 None:intergenic
ATATGAAATCCCAGTTACAT+TGG 0.575710 1.2:-72897469 None:intergenic
CCTCTTCGTATTAGGCCTGA+TGG 0.578703 1.2:+72895627 MS.gene54777:CDS
TTGGAACATTTGAGTACTGG+TGG 0.579804 1.2:+72897045 MS.gene54777:CDS
ACTGAAGCACCACTGAGTCA+TGG 0.585903 1.2:+72895519 MS.gene54777:CDS
GATACCACATCGCTCAGAAG+CGG 0.585913 1.2:+72897230 MS.gene54777:CDS
TCAGAATACCTTTCAGCACA+TGG 0.589369 1.2:-72897405 None:intergenic
CATATGATCCATGTGCTGAA+AGG 0.595074 1.2:+72897397 MS.gene54777:CDS
TTTGAGTACTGGTGGACACA+TGG 0.597648 1.2:+72897053 MS.gene54777:CDS
TCATATGCTCTATACATCCA+AGG 0.600430 1.2:-72897381 None:intergenic
GGGTACCAATTGATGATGGT+TGG 0.601605 1.2:-72898169 None:intergenic
TAGAGAATTCTACATGGCTG+GGG 0.605545 1.2:+72896698 MS.gene54777:CDS
AGGCTATGTGACTGTGAATG+AGG 0.607173 1.2:+72895467 MS.gene54777:CDS
TATATAGGAAGCATAGACAA+TGG 0.610027 1.2:-72897644 None:intergenic
AGTAAGAGGTGCTGGACATG+AGG 0.612576 1.2:+72898495 MS.gene54777:CDS
CAATCGTCCAGAAGTTCAGA+AGG 0.617406 1.2:+72897437 MS.gene54777:CDS
GTGACTGTGAATGAGGAGAG+TGG 0.618598 1.2:+72895474 MS.gene54777:CDS
ACATATACGAAGCTAAACAT+TGG 0.619718 1.2:+72897092 MS.gene54777:CDS
CAACACCAGCAGGAGACTCG+AGG 0.627276 1.2:-72895803 None:intergenic
ACCTTGCCATTATCATACCA+AGG 0.630379 1.2:-72898190 None:intergenic
GCTTCACGAGGCTTATGAAG+AGG 0.634672 1.2:-72898520 None:intergenic
GTATACCCATATCCGGAGAC+CGG 0.635543 1.2:-72897684 None:intergenic
AATTCTCTATGTTTGTACTG+AGG 0.640776 1.2:-72896684 None:intergenic
CAAACATAGAGAATTCTACA+TGG 0.646522 1.2:+72896692 MS.gene54777:CDS
AGTTTATAAAGGGCTCACAT+TGG 0.647641 1.2:+72898468 MS.gene54777:CDS
CCCTTGGTATGATAATGGCA+AGG 0.647659 1.2:+72898189 MS.gene54777:CDS
ATAAAATTAAGTGGTTACCA+GGG 0.651346 1.2:+72895412 MS.gene54777:CDS
TAAGCTATGGAAGAACAACC+GGG 0.663016 1.2:-72895582 None:intergenic
CCATTAAGCCATAAAACTAG+TGG 0.663685 1.2:-72895555 None:intergenic
CCATCAGGCCTAATACGAAG+AGG 0.675179 1.2:-72895627 None:intergenic
CCTTGCCATTATCATACCAA+GGG 0.676224 1.2:-72898189 None:intergenic
GAATTGGGTCCATGACTCAG+TGG 0.681910 1.2:-72895528 None:intergenic
TATAGGAAGCATAGACAATG+GGG 0.694959 1.2:-72897642 None:intergenic
TAAGCCGCTTCTGAGCGATG+TGG 0.719374 1.2:-72897234 None:intergenic
AAAAGAATAAATGCTTCACG+AGG 0.727158 1.2:-72898532 None:intergenic
GATGTGGTATCGTTACAAGG+CGG 0.831267 1.2:-72897218 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAACTTAATATTGTAT+TGG + chr1.2:72898293-72898312 MS.gene54777:intron 10.0%
!! GTAGTTAAATATATATTATT+AGG + chr1.2:72896338-72896357 MS.gene54777:intron 10.0%
!!! ACAATATTAAGTTTTTATAT+TGG - chr1.2:72898291-72898310 None:intergenic 10.0%
!!! GTTACTAATATTTATTATTT+TGG + chr1.2:72895755-72895774 MS.gene54777:intron 10.0%
!! ATTAAATTTAACCGATAAAT+GGG + chr1.2:72895999-72896018 MS.gene54777:intron 15.0%
!! ATTAATCACATGATAATATT+TGG + chr1.2:72896273-72896292 MS.gene54777:intron 15.0%
!! CATGTGATTAATAAATTTAT+TGG - chr1.2:72896265-72896284 None:intergenic 15.0%
!! TATTAAATTTAACCGATAAA+TGG + chr1.2:72895998-72896017 MS.gene54777:intron 15.0%
!! TATTCATTCTTCTTTATATT+TGG - chr1.2:72895957-72895976 None:intergenic 15.0%
!! TTAATAAGTTCTTGATATAT+AGG - chr1.2:72897662-72897681 None:intergenic 15.0%
!!! AAAACTTATGAATTTGTTTT+GGG + chr1.2:72896373-72896392 MS.gene54777:intron 15.0%
!!! AATCCAGTTATAAATTTTAA+GGG + chr1.2:72896884-72896903 MS.gene54777:CDS 15.0%
!!! ATTAATAAATTTATTGGTCT+TGG - chr1.2:72896259-72896278 None:intergenic 15.0%
!!! CATATATTTTTCTAGTTAAT+TGG + chr1.2:72896649-72896668 MS.gene54777:CDS 15.0%
!!! TAAAACTTATGAATTTGTTT+TGG + chr1.2:72896372-72896391 MS.gene54777:intron 15.0%
!!! TAATCCAGTTATAAATTTTA+AGG + chr1.2:72896883-72896902 MS.gene54777:CDS 15.0%
!!! TATAAATTTTAAGGGATTTA+TGG + chr1.2:72896892-72896911 MS.gene54777:CDS 15.0%
!! AAAAGAGAGATAAAATTAAG+TGG + chr1.2:72895403-72895422 MS.gene54777:CDS 20.0%
!! AATCCCTTAAAATTTATAAC+TGG - chr1.2:72896890-72896909 None:intergenic 20.0%
!! ACATGTATTTGGTATAAATT+TGG - chr1.2:72897782-72897801 None:intergenic 20.0%
!! ACTTCTACTAATATATGTTA+TGG - chr1.2:72897315-72897334 None:intergenic 20.0%
!! GATGATTATCATGATTATAT+TGG + chr1.2:72897026-72897045 MS.gene54777:CDS 20.0%
!! TTAAATTTAACCGATAAATG+GGG + chr1.2:72896000-72896019 MS.gene54777:intron 20.0%
!!! ATTCTATCATGATTTTGTAT+TGG + chr1.2:72897565-72897584 MS.gene54777:intron 20.0%
!!! GTTATTGTCAGTTTTAATTT+TGG - chr1.2:72896075-72896094 None:intergenic 20.0%
!!! TATTCTTTTTAGATCATTCT+TGG + chr1.2:72898546-72898565 MS.gene54777:CDS 20.0%
!!! TTGTTATTTGTTAGTTTGTT+TGG - chr1.2:72896162-72896181 None:intergenic 20.0%
!!! TTGTTTATATTTAAAACCAG+AGG - chr1.2:72897738-72897757 None:intergenic 20.0%
!!! TTTCTCTTTGTACTTATTTT+TGG + chr1.2:72896299-72896318 MS.gene54777:intron 20.0%
! AAAACTGACAATAACTGTTT+AGG + chr1.2:72896080-72896099 MS.gene54777:intron 25.0%
! AAGTATATAGTTAAGGTTGA+TGG - chr1.2:72897715-72897734 None:intergenic 25.0%
! AATAAACACAACAAAAGTGA+TGG - chr1.2:72897959-72897978 None:intergenic 25.0%
! AATCTCATCCATTTCATATT+CGG + chr1.2:72898036-72898055 MS.gene54777:intron 25.0%
! AATTGTTAACCAGAGAAATA+AGG + chr1.2:72896853-72896872 MS.gene54777:CDS 25.0%
! ATAAAATTAAGTGGTTACCA+GGG + chr1.2:72895412-72895431 MS.gene54777:CDS 25.0%
! ATAAACACAACAAAAGTGAT+GGG - chr1.2:72897958-72897977 None:intergenic 25.0%
! ATGAGATAATGAGATTATCA+AGG - chr1.2:72898412-72898431 None:intergenic 25.0%
! ATTGTTAACCAGAGAAATAA+GGG + chr1.2:72896854-72896873 MS.gene54777:CDS 25.0%
! TAAGTGAAATTTCGTTTCAT+AGG - chr1.2:72897851-72897870 None:intergenic 25.0%
! TATCAAGAACTTATTAATGC+CGG + chr1.2:72897665-72897684 MS.gene54777:CDS 25.0%
!! ATCAGTTTTCTACTTATCAA+TGG + chr1.2:72896752-72896771 MS.gene54777:intron 25.0%
!! CATAATTTTCTCTTTTGTGA+AGG + chr1.2:72898429-72898448 MS.gene54777:intron 25.0%
!! TATCATGATTTTGTATTGGA+AGG + chr1.2:72897569-72897588 MS.gene54777:intron 25.0%
!! TTTTCTACTTATCAATGGAA+TGG + chr1.2:72896757-72896776 MS.gene54777:intron 25.0%
!!! AAATAACAACGTCTTTTTCA+AGG + chr1.2:72896173-72896192 MS.gene54777:intron 25.0%
!!! AGCTAACATAAATTTTAGGA+GGG + chr1.2:72895921-72895940 MS.gene54777:intron 25.0%
!!! ATTTTGAAAGGATTGGTTAT+AGG + chr1.2:72898380-72898399 MS.gene54777:intron 25.0%
!!! ATTTTTGGCTTATAGTTGTT+TGG + chr1.2:72896314-72896333 MS.gene54777:intron 25.0%
!!! TATACTTTTTCCCCATTTAT+CGG - chr1.2:72896013-72896032 None:intergenic 25.0%
!!! TGTTCAAATCCAACATTTTT+TGG - chr1.2:72895441-72895460 None:intergenic 25.0%
!!! TTTTTGTCCATTTTTTGTGT+GGG + chr1.2:72895351-72895370 MS.gene54777:CDS 25.0%
AAAAGAATAAATGCTTCACG+AGG - chr1.2:72898535-72898554 None:intergenic 30.0%
AAACAACACAACACAGAATA+TGG - chr1.2:72895334-72895353 None:intergenic 30.0%
AATTCTCTATGTTTGTACTG+AGG - chr1.2:72896687-72896706 None:intergenic 30.0%
ACATATACGAAGCTAAACAT+TGG + chr1.2:72897092-72897111 MS.gene54777:CDS 30.0%
AGAAATTCCCACACAAAAAA+TGG - chr1.2:72895361-72895380 None:intergenic 30.0%
ATAATGAGATTATCAAGGCA+GGG - chr1.2:72898407-72898426 None:intergenic 30.0%
ATATGAAATCCCAGTTACAT+TGG - chr1.2:72897472-72897491 None:intergenic 30.0%
ATATTGGAACATTTGAGTAC+TGG + chr1.2:72897042-72897061 MS.gene54777:CDS 30.0%
CAAACATAGAGAATTCTACA+TGG + chr1.2:72896692-72896711 MS.gene54777:CDS 30.0%
CTAGTTTACCGAATATGAAA+TGG - chr1.2:72898047-72898066 None:intergenic 30.0%
CTTCTTTATATTTGGCCAAT+TGG - chr1.2:72895949-72895968 None:intergenic 30.0%
GATAAAATTAAGTGGTTACC+AGG + chr1.2:72895411-72895430 MS.gene54777:CDS 30.0%
GATGGAGTCAAGTTTATAAA+GGG + chr1.2:72898458-72898477 MS.gene54777:CDS 30.0%
GTTTGGTACAAAGTCAATTA+TGG - chr1.2:72896145-72896164 None:intergenic 30.0%
TAGATTTAAATGATTTCCCC+AGG + chr1.2:72896806-72896825 MS.gene54777:intron 30.0%
TCGCTAAAACAAAACAAATC+TGG - chr1.2:72896555-72896574 None:intergenic 30.0%
TGACGTGTTAATATGTATCA+GGG + chr1.2:72896582-72896601 MS.gene54777:intron 30.0%
TTACAATTGTTCCAAGCATA+AGG - chr1.2:72895672-72895691 None:intergenic 30.0%
TTGACGTGTTAATATGTATC+AGG + chr1.2:72896581-72896600 MS.gene54777:intron 30.0%
TTTACTTGAATCCTTATGCT+TGG + chr1.2:72895658-72895677 MS.gene54777:CDS 30.0%
! AATGTTTAGCTTCGTATATG+TGG - chr1.2:72897093-72897112 None:intergenic 30.0%
! ACGGAGCTAACATAAATTTT+AGG + chr1.2:72895917-72895936 MS.gene54777:intron 30.0%
! ATATAGGAAGCATAGACAAT+GGG - chr1.2:72897646-72897665 None:intergenic 30.0%
! GAGCTAACATAAATTTTAGG+AGG + chr1.2:72895920-72895939 MS.gene54777:intron 30.0%
! TATATAGGAAGCATAGACAA+TGG - chr1.2:72897647-72897666 None:intergenic 30.0%
! TCATAATCGCAGATTTTGAA+AGG + chr1.2:72898368-72898387 MS.gene54777:intron 30.0%
! TCTTCTGAACTTTCGTATTT+TGG + chr1.2:72898076-72898095 MS.gene54777:intron 30.0%
! TGAATGAGTTGAAAGCATAT+AGG - chr1.2:72897277-72897296 None:intergenic 30.0%
! TGCTTGTATTTAAGACTAGA+GGG + chr1.2:72897808-72897827 MS.gene54777:intron 30.0%
! TTGCTTGTATTTAAGACTAG+AGG + chr1.2:72897807-72897826 MS.gene54777:intron 30.0%
! TTTTTGTGTGGGAATTTCTT+TGG + chr1.2:72895362-72895381 MS.gene54777:CDS 30.0%
!! AAAACTAGTGGCTTTGAATT+GGG - chr1.2:72895546-72895565 None:intergenic 30.0%
!! CTAACATAAATTTTAGGAGG+GGG + chr1.2:72895923-72895942 MS.gene54777:intron 30.0%
!! GCTAACATAAATTTTAGGAG+GGG + chr1.2:72895922-72895941 MS.gene54777:intron 30.0%
!! TAAAACTAGTGGCTTTGAAT+TGG - chr1.2:72895547-72895566 None:intergenic 30.0%
!! TAAACATTGGCTGTGATTTT+GGG + chr1.2:72897105-72897124 MS.gene54777:CDS 30.0%
!! TCTAGTCTTAAATACAAGCA+AGG - chr1.2:72897808-72897827 None:intergenic 30.0%
!!! ATTCAAAGCCACTAGTTTTA+TGG + chr1.2:72895547-72895566 MS.gene54777:CDS 30.0%
!!! CGTTTTTTCTATCATGTCAA+AGG + chr1.2:72898234-72898253 MS.gene54777:intron 30.0%
!!! GTTTTTGTCCATTTTTTGTG+TGG + chr1.2:72895350-72895369 MS.gene54777:CDS 30.0%
!!! TATTATTTTGGATGTTGCAG+TGG + chr1.2:72895767-72895786 MS.gene54777:intron 30.0%
!!! TTTTTTCTTCTTCTTGACAG+TGG + chr1.2:72897593-72897612 MS.gene54777:intron 30.0%
AAATTGGTCCTCTTCGTATT+AGG + chr1.2:72895619-72895638 MS.gene54777:CDS 35.0%
AACCAGAGAAATAAGGGAAT+CGG + chr1.2:72896860-72896879 MS.gene54777:CDS 35.0%
AACTGAAGAACATAATGACC+TGG - chr1.2:72896827-72896846 None:intergenic 35.0%
ACAACAGATATGCAGACATT+TGG + chr1.2:72895846-72895865 MS.gene54777:CDS 35.0%
ACTGAAGAACATAATGACCT+GGG - chr1.2:72896826-72896845 None:intergenic 35.0%
ACTGCAACTCGATATTCTAT+TGG + chr1.2:72898133-72898152 MS.gene54777:CDS 35.0%
AGGTCATTATGTTCTTCAGT+TGG + chr1.2:72896826-72896845 MS.gene54777:intron 35.0%
AGTTTATAAAGGGCTCACAT+TGG + chr1.2:72898468-72898487 MS.gene54777:CDS 35.0%
ATAAGCTATGGAAGAACAAC+CGG - chr1.2:72895586-72895605 None:intergenic 35.0%
ATAGAGAATTCTACATGGCT+GGG + chr1.2:72896697-72896716 MS.gene54777:CDS 35.0%
CCATTAAGCCATAAAACTAG+TGG - chr1.2:72895558-72895577 None:intergenic 35.0%
CCTTAACTATATACTTCCTC+TGG + chr1.2:72897719-72897738 MS.gene54777:intron 35.0%
GATAATGAGATTATCAAGGC+AGG - chr1.2:72898408-72898427 None:intergenic 35.0%
GGATGGAGTCAAGTTTATAA+AGG + chr1.2:72898457-72898476 MS.gene54777:CDS 35.0%
GTTGGATTTGAACAGTATTC+AGG + chr1.2:72895447-72895466 MS.gene54777:CDS 35.0%
TAACTGGGATTTCATATGCT+TGG + chr1.2:72897475-72897494 MS.gene54777:CDS 35.0%
TAATGAGATTATCAAGGCAG+GGG - chr1.2:72898406-72898425 None:intergenic 35.0%
TATTCTACTATCGTTGCAAC+AGG + chr1.2:72896986-72897005 MS.gene54777:intron 35.0%
TCATATGCTCTATACATCCA+AGG - chr1.2:72897384-72897403 None:intergenic 35.0%
TGACACTCGTGATTAAGAAA+TGG - chr1.2:72897994-72898013 None:intergenic 35.0%
! AAATGGGGAAAAAGTATACG+CGG + chr1.2:72896015-72896034 MS.gene54777:intron 35.0%
! CATAATAAGTGTCACGCTTT+TGG - chr1.2:72898268-72898287 None:intergenic 35.0%
! CCAGAGGAAGTATATAGTTA+AGG - chr1.2:72897722-72897741 None:intergenic 35.0%
! TATAGGAAGCATAGACAATG+GGG - chr1.2:72897645-72897664 None:intergenic 35.0%
! TATGGTGCTTCTGAAGAAAT+TGG + chr1.2:72895603-72895622 MS.gene54777:CDS 35.0%
! TTCTCTTTTGTGAAGGTTTG+TGG + chr1.2:72898436-72898455 MS.gene54777:intron 35.0%
!! AGTAGTAGTGTTGATATGTC+TGG - chr1.2:72896446-72896465 None:intergenic 35.0%
!! ATCGCAGATTTTGAAAGGAT+TGG + chr1.2:72898373-72898392 MS.gene54777:intron 35.0%
!! CTAAACATTGGCTGTGATTT+TGG + chr1.2:72897104-72897123 MS.gene54777:CDS 35.0%
!! TGTAATGGTTTTGCTCATGA+AGG + chr1.2:72896515-72896534 MS.gene54777:intron 35.0%
!!! CCACTAGTTTTATGGCTTAA+TGG + chr1.2:72895555-72895574 MS.gene54777:CDS 35.0%
!!! CTAGTTTTATGGCTTAATGG+TGG + chr1.2:72895558-72895577 MS.gene54777:CDS 35.0%
!!! TTTTTTTGACTTTGCAGCTG+AGG + chr1.2:72896623-72896642 MS.gene54777:intron 35.0%
AACTTATTAATGCCGGTCTC+CGG + chr1.2:72897672-72897691 MS.gene54777:CDS 40.0%
ACCTTGCCATTATCATACCA+AGG - chr1.2:72898193-72898212 None:intergenic 40.0%
ACTGAGCAAGGAAACATTGA+TGG + chr1.2:72897179-72897198 MS.gene54777:CDS 40.0%
ACTGTTGCAATTACTGAGCA+AGG + chr1.2:72897167-72897186 MS.gene54777:CDS 40.0%
AGCTACCAACCATCATCAAT+TGG + chr1.2:72898164-72898183 MS.gene54777:CDS 40.0%
ATTCGAGCCATTATCAGTGT+GGG + chr1.2:72898325-72898344 MS.gene54777:intron 40.0%
CACTTCATGCCAATGTAACT+GGG + chr1.2:72897460-72897479 MS.gene54777:CDS 40.0%
CATAGAGAATTCTACATGGC+TGG + chr1.2:72896696-72896715 MS.gene54777:CDS 40.0%
CATATGATCCATGTGCTGAA+AGG + chr1.2:72897397-72897416 MS.gene54777:CDS 40.0%
CCTTGCCATTATCATACCAA+GGG - chr1.2:72898192-72898211 None:intergenic 40.0%
CTATCATGTCAAAGGCGATA+AGG + chr1.2:72898242-72898261 MS.gene54777:intron 40.0%
CTATGAAGCACAGACAATGA+CGG + chr1.2:72895898-72895917 MS.gene54777:intron 40.0%
CTGAAGAACATAATGACCTG+GGG - chr1.2:72896825-72896844 None:intergenic 40.0%
GGTGATGCATAGGTTTGTAA+TGG + chr1.2:72896500-72896519 MS.gene54777:intron 40.0%
GGTTGTTCTTCCATAGCTTA+TGG + chr1.2:72895585-72895604 MS.gene54777:CDS 40.0%
TAAGCTATGGAAGAACAACC+GGG - chr1.2:72895585-72895604 None:intergenic 40.0%
TAGAGAATTCTACATGGCTG+GGG + chr1.2:72896698-72896717 MS.gene54777:CDS 40.0%
TCAGAATACCTTTCAGCACA+TGG - chr1.2:72897408-72897427 None:intergenic 40.0%
TCTTGACAGTGGTACTGTTT+GGG + chr1.2:72897604-72897623 MS.gene54777:intron 40.0%
TGGGATTTCATATGCTTGGA+AGG + chr1.2:72897479-72897498 MS.gene54777:CDS 40.0%
TGGTACCCTTGGTATGATAA+TGG + chr1.2:72898184-72898203 MS.gene54777:CDS 40.0%
TTCAAGTCCCACACTGATAA+TGG - chr1.2:72898335-72898354 None:intergenic 40.0%
TTCTTGACAGTGGTACTGTT+TGG + chr1.2:72897603-72897622 MS.gene54777:intron 40.0%
! AAGTAAAGTGACTTGCCATC+AGG - chr1.2:72895645-72895664 None:intergenic 40.0%
! ACATTTGGTGACCAGAAAAC+AGG + chr1.2:72895861-72895880 MS.gene54777:CDS 40.0%
! CGACTACATTACCTGTTTTC+TGG - chr1.2:72895875-72895894 None:intergenic 40.0%
! CTTTTGTGAAGGTTTGTGGA+TGG + chr1.2:72898440-72898459 MS.gene54777:intron 40.0%
! GATATGTCTGGTGTCAATGT+CGG - chr1.2:72896434-72896453 None:intergenic 40.0%
! GTGGTACTGTTTGGGATTAT+TGG + chr1.2:72897612-72897631 MS.gene54777:intron 40.0%
! TGGTCTTGGTTAGACACTTT+CGG - chr1.2:72896245-72896264 None:intergenic 40.0%
!! TACCGATTCCCTTATTTCTC+TGG - chr1.2:72896865-72896884 None:intergenic 40.0%
!! TGGTATGATAATGGCAAGGT+TGG + chr1.2:72898193-72898212 MS.gene54777:intron 40.0%
!! TTCAGAAGCACCATAAGCTA+TGG - chr1.2:72895598-72895617 None:intergenic 40.0%
!! TTGGAACATTTGAGTACTGG+TGG + chr1.2:72897045-72897064 MS.gene54777:CDS 40.0%
!!! GTGGAAGAGCATTGTTTTAC+TGG + chr1.2:72895493-72895512 MS.gene54777:CDS 40.0%
AAGCAAGGCGCACATGTATT+TGG - chr1.2:72897793-72897812 None:intergenic 45.0%
AGCGATGTGGTATCGTTACA+AGG - chr1.2:72897224-72897243 None:intergenic 45.0%
AGGCTATGTGACTGTGAATG+AGG + chr1.2:72895467-72895486 MS.gene54777:CDS 45.0%
ATACATCCAAGGCTGAAGAG+AGG - chr1.2:72897373-72897392 None:intergenic 45.0%
CAATCGTCCAGAAGTTCAGA+AGG + chr1.2:72897437-72897456 MS.gene54777:CDS 45.0%
CACATGTGTCAGTGTTGTGT+CGG + chr1.2:72896479-72896498 MS.gene54777:intron 45.0%
CATTCGAGCCATTATCAGTG+TGG + chr1.2:72898324-72898343 MS.gene54777:intron 45.0%
CCAGGGCAACCAAAAAATGT+TGG + chr1.2:72895429-72895448 MS.gene54777:CDS 45.0%
CCATCATCAATTGGTACCCT+TGG + chr1.2:72898173-72898192 MS.gene54777:CDS 45.0%
CTACTATCGTTGCAACAGGT+TGG + chr1.2:72896990-72897009 MS.gene54777:intron 45.0%
CTCACATTGGTCACAGTAAG+AGG + chr1.2:72898481-72898500 MS.gene54777:CDS 45.0%
GATGTGGTATCGTTACAAGG+CGG - chr1.2:72897221-72897240 None:intergenic 45.0%
GCACTTCATGCCAATGTAAC+TGG + chr1.2:72897459-72897478 MS.gene54777:CDS 45.0%
GGTACCTGTATACCCATATC+CGG - chr1.2:72897694-72897713 None:intergenic 45.0%
TAAGAAAAGCCAACACCAGC+AGG - chr1.2:72895816-72895835 None:intergenic 45.0%
TACATCCAAGGCTGAAGAGA+GGG - chr1.2:72897372-72897391 None:intergenic 45.0%
TTATGGCTTAATGGTGGTCC+CGG + chr1.2:72895564-72895583 MS.gene54777:CDS 45.0%
! GAATATCGAGTTGCAGTCAG+TGG - chr1.2:72898130-72898149 None:intergenic 45.0%
! TCGTTGCAACAGGTTGGAAA+TGG + chr1.2:72896996-72897015 MS.gene54777:intron 45.0%
! TGAAGTGCCTTCTGAACTTC+TGG - chr1.2:72897447-72897466 None:intergenic 45.0%
! TTTTTCCCTCTCTTCAGCCT+TGG + chr1.2:72897364-72897383 MS.gene54777:intron 45.0%
!! AATTTTAGGAGGGGGCCAAT+TGG + chr1.2:72895931-72895950 MS.gene54777:intron 45.0%
!! CACACTGATAATGGCTCGAA+TGG - chr1.2:72898326-72898345 None:intergenic 45.0%
!! CCAAGGGTACCAATTGATGA+TGG - chr1.2:72898176-72898195 None:intergenic 45.0%
!! CCCTTGGTATGATAATGGCA+AGG + chr1.2:72898189-72898208 MS.gene54777:CDS 45.0%
!! GATGATGGTTGGTAGCTTCA+AGG - chr1.2:72898161-72898180 None:intergenic 45.0%
!! GGGTACCAATTGATGATGGT+TGG - chr1.2:72898172-72898191 None:intergenic 45.0%
!! GTCGGTGTCTATTTTGTGCT+CGG - chr1.2:72896416-72896435 None:intergenic 45.0%
!! TCGGTGTCTATTTTGTGCTC+GGG - chr1.2:72896415-72896434 None:intergenic 45.0%
!! TTTGAGTACTGGTGGACACA+TGG + chr1.2:72897053-72897072 MS.gene54777:CDS 45.0%
!!! CCAACATTTTTTGGTTGCCC+TGG - chr1.2:72895432-72895451 None:intergenic 45.0%
!!! CTTTCGTATTTTGGCTGCAG+TGG + chr1.2:72898085-72898104 MS.gene54777:intron 45.0%
!!! GAATTTGTTTTGGGCCGTCA+AGG + chr1.2:72896382-72896401 MS.gene54777:intron 45.0%
ATGGCTGGGGAAAGTTATGC+AGG + chr1.2:72896711-72896730 MS.gene54777:CDS 50.0%
CCATCAGGCCTAATACGAAG+AGG - chr1.2:72895630-72895649 None:intergenic 50.0%
CCTCTTCGTATTAGGCCTGA+TGG + chr1.2:72895627-72895646 MS.gene54777:CDS 50.0%
CGACACAACACTGACACATG+TGG - chr1.2:72896481-72896500 None:intergenic 50.0%
GAATTGGGTCCATGACTCAG+TGG - chr1.2:72895531-72895550 None:intergenic 50.0%
GATACCACATCGCTCAGAAG+CGG + chr1.2:72897230-72897249 MS.gene54777:CDS 50.0%
GCTTCACGAGGCTTATGAAG+AGG - chr1.2:72898523-72898542 None:intergenic 50.0%
GTATACCCATATCCGGAGAC+CGG - chr1.2:72897687-72897706 None:intergenic 50.0%
GTCTCCGGATATGGGTATAC+AGG + chr1.2:72897687-72897706 MS.gene54777:CDS 50.0%
GTGACTGTGAATGAGGAGAG+TGG + chr1.2:72895474-72895493 MS.gene54777:CDS 50.0%
TAATGCCGGTCTCCGGATAT+GGG + chr1.2:72897679-72897698 MS.gene54777:CDS 50.0%
TCCATCGGTGCAATGTTTGC+AGG + chr1.2:72897139-72897158 MS.gene54777:CDS 50.0%
TGGGTCCATTCAGCATCCAT+CGG + chr1.2:72897124-72897143 MS.gene54777:CDS 50.0%
TTAATGCCGGTCTCCGGATA+TGG + chr1.2:72897678-72897697 MS.gene54777:CDS 50.0%
! ACTGAAGCACCACTGAGTCA+TGG + chr1.2:72895519-72895538 MS.gene54777:CDS 50.0%
! CATATGCTTGGAAGGCATGC+AGG + chr1.2:72897487-72897506 MS.gene54777:CDS 50.0%
! CGGTGTCTATTTTGTGCTCG+GGG - chr1.2:72896414-72896433 None:intergenic 50.0%
! GGTGTCTATTTTGTGCTCGG+GGG - chr1.2:72896413-72896432 None:intergenic 50.0%
! GTGTTGTGTCGGTGATGCAT+AGG + chr1.2:72896490-72896509 MS.gene54777:intron 50.0%
! TTGCACCGATGGATGCTGAA+TGG - chr1.2:72897132-72897151 None:intergenic 50.0%
! TTGGTCACAGTAAGAGGTGC+TGG + chr1.2:72898487-72898506 MS.gene54777:CDS 50.0%
!! AGTAAGAGGTGCTGGACATG+AGG + chr1.2:72898495-72898514 MS.gene54777:CDS 50.0%
GCCTGCAAACATTGCACCGA+TGG - chr1.2:72897143-72897162 None:intergenic 55.0%
TAAGCCGCTTCTGAGCGATG+TGG - chr1.2:72897237-72897256 None:intergenic 55.0%
TCGACGAATCGAAGCGACGA+GGG - chr1.2:72895722-72895741 None:intergenic 55.0%
TTCGACGAATCGAAGCGACG+AGG - chr1.2:72895723-72895742 None:intergenic 55.0%
CAACACCAGCAGGAGACTCG+AGG - chr1.2:72895806-72895825 None:intergenic 60.0%
CTCGAGTCTCCTGCTGGTGT+TGG + chr1.2:72895804-72895823 MS.gene54777:CDS 60.0%
CTGTTCCTCGAGTCTCCTGC+TGG + chr1.2:72895798-72895817 MS.gene54777:CDS 60.0%
! GCTCGGGGGCGTAGCCTTGA+CGG - chr1.2:72896399-72896418 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 72895321 72898594 72895321 ID=MS.gene54777
chr1.2 mRNA 72895321 72898594 72895321 ID=MS.gene54777.t1;Parent=MS.gene54777
chr1.2 exon 72895321 72895687 72895321 ID=MS.gene54777.t1.exon1;Parent=MS.gene54777.t1
chr1.2 CDS 72895321 72895687 72895321 ID=cds.MS.gene54777.t1;Parent=MS.gene54777.t1
chr1.2 exon 72895787 72895882 72895787 ID=MS.gene54777.t1.exon2;Parent=MS.gene54777.t1
chr1.2 CDS 72895787 72895882 72895787 ID=cds.MS.gene54777.t1;Parent=MS.gene54777.t1
chr1.2 exon 72896640 72896732 72896640 ID=MS.gene54777.t1.exon3;Parent=MS.gene54777.t1
chr1.2 CDS 72896640 72896732 72896640 ID=cds.MS.gene54777.t1;Parent=MS.gene54777.t1
chr1.2 exon 72896828 72896913 72896828 ID=MS.gene54777.t1.exon4;Parent=MS.gene54777.t1
chr1.2 CDS 72896828 72896913 72896828 ID=cds.MS.gene54777.t1;Parent=MS.gene54777.t1
chr1.2 exon 72897008 72897268 72897008 ID=MS.gene54777.t1.exon5;Parent=MS.gene54777.t1
chr1.2 CDS 72897008 72897268 72897008 ID=cds.MS.gene54777.t1;Parent=MS.gene54777.t1
chr1.2 exon 72897381 72897508 72897381 ID=MS.gene54777.t1.exon6;Parent=MS.gene54777.t1
chr1.2 CDS 72897381 72897508 72897381 ID=cds.MS.gene54777.t1;Parent=MS.gene54777.t1
chr1.2 exon 72897613 72897708 72897613 ID=MS.gene54777.t1.exon7;Parent=MS.gene54777.t1
chr1.2 CDS 72897613 72897708 72897613 ID=cds.MS.gene54777.t1;Parent=MS.gene54777.t1
chr1.2 exon 72898105 72898210 72898105 ID=MS.gene54777.t1.exon8;Parent=MS.gene54777.t1
chr1.2 CDS 72898105 72898210 72898105 ID=cds.MS.gene54777.t1;Parent=MS.gene54777.t1
chr1.2 exon 72898451 72898594 72898451 ID=MS.gene54777.t1.exon9;Parent=MS.gene54777.t1
chr1.2 CDS 72898451 72898594 72898451 ID=cds.MS.gene54777.t1;Parent=MS.gene54777.t1
Gene Sequence

>MS.gene54777

ATGAGTCATTCCATATTCTGTGTTGTGTTGTTTTTGTCCATTTTTTGTGTGGGAATTTCTTTGGCTTCTTCTATAAAAGAACAAAAGAGAGATAAAATTAAGTGGTTACCAGGGCAACCAAAAAATGTTGGATTTGAACAGTATTCAGGCTATGTGACTGTGAATGAGGAGAGTGGAAGAGCATTGTTTTACTGGTTGACTGAAGCACCACTGAGTCATGGACCCAATTCAAAGCCACTAGTTTTATGGCTTAATGGTGGTCCCGGTTGTTCTTCCATAGCTTATGGTGCTTCTGAAGAAATTGGTCCTCTTCGTATTAGGCCTGATGGCAAGTCACTTTACTTGAATCCTTATGCTTGGAACAATTGTAAGTCAAATTCTCATCTAACGAAATTGAACCCTCGTCGCTTCGATTCGTCGAACTAAAAAATATCGTTACTAATATTTATTATTTTGGATGTTGCAGTGGCAAACATGCTGTTCCTCGAGTCTCCTGCTGGTGTTGGCTTTTCTTATTGTAATAAAACAACAGATATGCAGACATTTGGTGACCAGAAAACAGGTAATGTAGTCGTAACTATGAAGCACAGACAATGACGGAGCTAACATAAATTTTAGGAGGGGGCCAATTGGCCAAATATAAAGAAGAATGAATAAACTACATTTAAAAACTAATATATTAAATTTAACCGATAAATGGGGAAAAAGTATACGCGGTAAATTTCAGAAGAATTTTTTTCTTATATAAAGTCCAAAATTAAAACTGACAATAACTGTTTAGGAAAGTTGAGCTCGACAACACGTCTAACAGTCAAATTCATCCATAATTGACTTTGTACCAAACAAACTAACAAATAACAACGTCTTTTTCAAGGTAAACAGCTGATCTATTTGCAAGAAACACTATAGTTGCTCAAGAGTCCGAAAGTGTCTAACCAAGACCAATAAATTTATTAATCACATGATAATATTTGGACTTTTCTCTTTGTACTTATTTTTGGCTTATAGTTGTTTGGAGTAGTTAAATATATATTATTAGGCTAGCTAAATATAAAACTTATGAATTTGTTTTGGGCCGTCAAGGCTACGCCCCCGAGCACAAAATAGACACCGACATTGACACCAGACATATCAACACTACTACTAACTGAATGTAGCCACATGTGTCAGTGTTGTGTCGGTGATGCATAGGTTTGTAATGGTTTTGCTCATGAAGGATTTACTCGCAGTGCCAGATTTGTTTTGTTTTAGCGATGAGATTTGACGTGTTAATATGTATCAGGGATCTGTAATTTTTTTTTTTTTTTTTGACTTTGCAGCTGAGGATGCATATATTTTTCTAGTTAATTGGTTCAAAAGATTTCCTCAGTACAAACATAGAGAATTCTACATGGCTGGGGAAAGTTATGCAGGTGAATCTATATTTGATATATCAGTTTTCTACTTATCAATGGAATGGCTAATGAAGAATTGTTCAATTTCTTATAGATTTAAATGATTTCCCCAGGTCATTATGTTCTTCAGTTGGCTCAAATTGTTAACCAGAGAAATAAGGGAATCGGTAATCCAGTTATAAATTTTAAGGGATTTATGGTAAGCTTTTTAGATTTATGTTTTAATCTTTTATGTTCACATAGACAGAACACAACTAACTTATGATAATCATATTCTACTATCGTTGCAACAGGTTGGAAATGGTGTGATTGATGATTATCATGATTATATTGGAACATTTGAGTACTGGTGGACACATGGTTTGATTTCAGATTCCACATATACGAAGCTAAACATTGGCTGTGATTTTGGGTCCATTCAGCATCCATCGGTGCAATGTTTGCAGGCTCTTACTGTTGCAATTACTGAGCAAGGAAACATTGATGGATATAGCATTAACACACCGCCTTGTAACGATACCACATCGCTCAGAAGCGGCTTACATGATCGCTATGTAAGCCTATATGCTTTCAACTCATTCACATTGCTTACAAAAACCATAACATATATTAGTAGAAGTGTTTTGATGATTTTTTTTTTTTCGTTTCTTTTTTCCCTCTCTTCAGCCTTGGATGTATAGAGCATATGATCCATGTGCTGAAAGGTATTCTGATGTGTACTTCAATCGTCCAGAAGTTCAGAAGGCACTTCATGCCAATGTAACTGGGATTTCATATGCTTGGAAGGCATGCAGGTATATTAATTTTAATTTAGTAAATTGATATGTAACATGTTCTATTGAAGTCTATAATTCTATCATGATTTTGTATTGGAAGGTTTTTTTCTTCTTCTTGACAGTGGTACTGTTTGGGATTATTGGACTGATTCCCCATTGTCTATGCTTCCTATATATCAAGAACTTATTAATGCCGGTCTCCGGATATGGGTATACAGGTACCATCAACCTTAACTATATACTTCCTCTGGTTTTAAATATAAACAAAAAGTTACATTTATATCTGATCCAAATTTATACCAAATACATGTGCGCCTTGCTTGTATTTAAGACTAGAGGGAGTATTTGCGTTTGACTCCTATGAAACGAAATTTCACTTAAAAGTATGAAGCAAGATATATATCTCAACACTTGTCAAAATAATTCATATGATCTAGTGTGAATTACTTATTTATGAGTCTAATCCCATCACTTTTGTTGTGTTTATTAGAAGCATTGTGCCATTTCTTAATCACGAGTGTCACATATCATGTTTTTATTTCAGCAATCTCATCCATTTCATATTCGGTAAACTAGAACTCGAATTCTTCTGAACTTTCGTATTTTGGCTGCAGTGGAGACACTGATGCTGTAATTCCACTGACTGCAACTCGATATTCTATTGGTGCCTTGAAGCTACCAACCATCATCAATTGGTACCCTTGGTATGATAATGGCAAGGTTGGTGATAACTTCTATCGTGTCGTTTTTTCTATCATGTCAAAGGCGATAAGGCCAAAAGCGTGACACTTATTATGCCAATATAAAAACTTAATATTGTATTGGAGCTTATCCATTCGAGCCATTATCAGTGTGGGACTTGAATAATCTCATTATCTCATAATCGCAGATTTTGAAAGGATTGGTTATAGGCCCCTGCCTTGATAATCTCATTATCTCATAATTTTCTCTTTTGTGAAGGTTTGTGGATGGAGTCAAGTTTATAAAGGGCTCACATTGGTCACAGTAAGAGGTGCTGGACATGAGGTTCCTCTTCATAAGCCTCGTGAAGCATTTATTCTTTTTAGATCATTCTTGGAAAACAAGAATATGCCATCAAGTTAA
Protein sequence

>MS.gene54777.t1

MSHSIFCVVLFLSIFCVGISLASSIKEQKRDKIKWLPGQPKNVGFEQYSGYVTVNEESGRALFYWLTEAPLSHGPNSKPLVLWLNGGPGCSSIAYGASEEIGPLRIRPDGKSLYLNPYAWNNLANMLFLESPAGVGFSYCNKTTDMQTFGDQKTAEDAYIFLVNWFKRFPQYKHREFYMAGESYAGHYVLQLAQIVNQRNKGIGNPVINFKGFMVGNGVIDDYHDYIGTFEYWWTHGLISDSTYTKLNIGCDFGSIQHPSVQCLQALTVAITEQGNIDGYSINTPPCNDTTSLRSGLHDRYPWMYRAYDPCAERYSDVYFNRPEVQKALHANVTGISYAWKACSGTVWDYWTDSPLSMLPIYQELINAGLRIWVYSGDTDAVIPLTATRYSIGALKLPTIINWYPWYDNGKVCGWSQVYKGLTLVTVRGAGHEVPLHKPREAFILFRSFLENKNMPSS