AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54858


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54858.t1 MTR_2g091180 91.239 331 29 0 1 331 1 331 0.0 625
MS.gene54858.t1 MTR_2g091180 91.239 331 29 0 1 331 34 364 0.0 623
MS.gene54858.t1 MTR_2g091180 91.239 331 29 0 1 331 35 365 0.0 623
MS.gene54858.t1 MTR_2g091180 89.124 331 29 1 1 331 35 358 0.0 604
MS.gene54858.t1 MTR_2g091180 88.839 224 25 0 1 224 35 258 1.03e-143 407
MS.gene54858.t1 MTR_4g079720 41.854 356 163 7 1 327 42 382 2.71e-70 224
MS.gene54858.t1 MTR_4g079720 41.292 356 159 7 1 327 42 376 2.55e-69 221
MS.gene54858.t1 MTR_5g052190 78.409 88 19 0 111 198 570 657 4.58e-35 135
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 55 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACGTTGAAATGACTTCTT+AGG 0.149183 2.1:-9752322 None:intergenic
TTGCATGGGAACAATGAAAA+TGG 0.296806 2.1:+9753571 MS.gene54858:CDS
TCAAGTATGAATCATGATTC+TGG 0.348680 2.1:+9752391 MS.gene54858:CDS
GTACTATGAGAACAATCTTC+AGG 0.406202 2.1:-9752930 None:intergenic
GCATTAGCATATTGACCTAT+AGG 0.407900 2.1:-9752289 None:intergenic
CTTTGTATTAACCGTTGCCT+TGG 0.433232 2.1:-9753387 None:intergenic
TATTGACCTATAGGCAATAC+AGG 0.434816 2.1:-9752280 None:intergenic
GAGACATCATCCTGCGAAGT+TGG 0.437673 2.1:-9753622 None:intergenic
TGAGTGCTCGGTTGATCGCT+TGG 0.441160 2.1:-9752371 None:intergenic
GCGACTTCACTGGCTGTCTT+CGG 0.444331 2.1:-9752529 None:intergenic
AAACCGCGGTATTCCCGTAT+TGG 0.446464 2.1:+9752714 MS.gene54858:CDS
TTTGTATTAACCGTTGCCTT+GGG 0.446863 2.1:-9753386 None:intergenic
AGATTTCTTATACAAAGAAA+AGG 0.463264 2.1:-9753515 None:intergenic
AACTAACTGAAGTTCGTCTC+TGG 0.466709 2.1:-9753363 None:intergenic
GGCGTGCAAAACAACATGTT+CGG 0.468393 2.1:+9752443 MS.gene54858:CDS
AACGTTGAAATGACTTCTTA+GGG 0.471471 2.1:-9752321 None:intergenic
TCACTGGCTGTCTTCGGTGC+AGG 0.473319 2.1:-9752523 None:intergenic
GCTACCCCTGTATTGCCTAT+AGG 0.475822 2.1:+9752274 MS.gene54858:CDS
TTCAGCTGAATCAACCAATA+CGG 0.476111 2.1:-9752728 None:intergenic
GAGTTTATGTCCAACTTCGC+AGG 0.480137 2.1:+9753612 MS.gene54858:CDS
ATACAGAGAAGTTCCCATTC+GGG 0.483134 2.1:-9753649 None:intergenic
ATGATTCATACTTGAGTGCT+CGG 0.490637 2.1:-9752383 None:intergenic
TTCCAAGAAAAGAATGGTAA+AGG 0.491376 2.1:+9752342 MS.gene54858:CDS
GAGAATCAAATGCAGATTGT+CGG 0.494930 2.1:+9752190 None:intergenic
TCAACGTTCCAAGAAAAGAA+TGG 0.498812 2.1:+9752336 MS.gene54858:CDS
TGATGTCTCTGAACCCGAAT+GGG 0.499518 2.1:+9753636 MS.gene54858:CDS
GATTTCTTATACAAAGAAAA+GGG 0.503803 2.1:-9753514 None:intergenic
TATACAGAGAAGTTCCCATT+CGG 0.509868 2.1:-9753650 None:intergenic
GAAGAGCTTGCATTATTGCA+TGG 0.512750 2.1:+9753556 MS.gene54858:CDS
GGGAACAATGAAAATGGAAA+TGG 0.513366 2.1:+9753577 MS.gene54858:CDS
CAAGTCGTTGTCTTGTCTCG+TGG 0.515765 2.1:-9753312 None:intergenic
AAGAGCTTGCATTATTGCAT+GGG 0.541758 2.1:+9753557 MS.gene54858:CDS
TCAGCTGAATCAACCAATAC+GGG 0.546541 2.1:-9752727 None:intergenic
ACAATCGCTGTGTCTCATCC+AGG 0.556728 2.1:+9752951 MS.gene54858:CDS
ACAGGGGTAGCACATGAAGA+TGG 0.556868 2.1:-9752262 None:intergenic
CAGATTGTCGGAGAAAGTGT+TGG 0.564686 2.1:+9752202 MS.gene54858:CDS
AAAGTTCTACTCAGAAGTCA+TGG 0.568093 2.1:+9752225 MS.gene54858:CDS
CTTTGTATAAGAAATCTACA+AGG 0.568921 2.1:+9753521 MS.gene54858:CDS
TCAGTTAGTTCCCAAGGCAA+CGG 0.569255 2.1:+9753376 MS.gene54858:CDS
ACAATCTGCTACAGCTGCAA+AGG 0.574614 2.1:-9752659 None:intergenic
CGAACTTCAGTTAGTTCCCA+AGG 0.579450 2.1:+9753370 MS.gene54858:CDS
ATGATGTCTCTGAACCCGAA+TGG 0.585604 2.1:+9753635 MS.gene54858:CDS
GCTCGAGGAAGTTTGTGTCA+TGG 0.603153 2.1:+9753337 MS.gene54858:CDS
ATTGACCTATAGGCAATACA+GGG 0.623800 2.1:-9752279 None:intergenic
CAATGTAAATGAAAATCATG+AGG 0.632995 2.1:+9752600 MS.gene54858:CDS
AGACAACGACTTGAAGCTCG+AGG 0.652614 2.1:+9753322 MS.gene54858:CDS
AAATGGAAATGGTGATTGCG+TGG 0.672357 2.1:+9753588 MS.gene54858:CDS
GGTTAATACAAAGAAGAGAG+AGG 0.674515 2.1:+9753397 MS.gene54858:CDS
GGGGTAGCACATGAAGATGG+AGG 0.680389 2.1:-9752259 None:intergenic
GACATACTTGAAGATGCACA+TGG 0.688594 2.1:+9752778 MS.gene54858:CDS
CAACCAATACGGGAATACCG+CGG 0.698495 2.1:-9752717 None:intergenic
GGGAACTTCTCTGTATACCA+CGG 0.703402 2.1:+9753656 MS.gene54858:CDS
TTGACCTATAGGCAATACAG+GGG 0.763489 2.1:-9752278 None:intergenic
GCAAGTACTGAACAAAACCG+CGG 0.773893 2.1:+9752700 MS.gene54858:CDS
CACCTACAACATGATCACCG+TGG 0.789761 2.1:-9753673 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAAAAAGAATACTTTTATT+TGG + chr2.1:9752422-9752441 MS.gene54858:CDS 15.0%
!! AGATTTCTTATACAAAGAAA+AGG - chr2.1:9753518-9753537 None:intergenic 20.0%
!! GATTTCTTATACAAAGAAAA+GGG - chr2.1:9753517-9753536 None:intergenic 20.0%
! ACGTGCATAAAAAATAGAAA+AGG - chr2.1:9753437-9753456 None:intergenic 25.0%
! CAATGTAAATGAAAATCATG+AGG + chr2.1:9752600-9752619 MS.gene54858:CDS 25.0%
! CGTGCATAAAAAATAGAAAA+GGG - chr2.1:9753436-9753455 None:intergenic 25.0%
! CTTTGTATAAGAAATCTACA+AGG + chr2.1:9753521-9753540 MS.gene54858:CDS 25.0%
! GTGCATAAAAAATAGAAAAG+GGG - chr2.1:9753435-9753454 None:intergenic 25.0%
!! ATGTGTATTGAGACAATTTT+AGG - chr2.1:9753219-9753238 None:intergenic 25.0%
!! CTCATGATTTTCATTTACAT+TGG - chr2.1:9752602-9752621 None:intergenic 25.0%
!! CTGAAGTACTAATTGAATTT+AGG + chr2.1:9752991-9753010 MS.gene54858:intron 25.0%
!! TGTGTATTGAGACAATTTTA+GGG - chr2.1:9753218-9753237 None:intergenic 25.0%
!!! ATATTCATTTTAACCCTGAT+TGG + chr2.1:9753243-9753262 MS.gene54858:intron 25.0%
AACGTTGAAATGACTTCTTA+GGG - chr2.1:9752324-9752343 None:intergenic 30.0%
ACTTCGTCTAATTAAGAACA+TGG - chr2.1:9753120-9753139 None:intergenic 30.0%
AGAAGTTCTTGCTTAGAATT+TGG + chr2.1:9753074-9753093 MS.gene54858:intron 30.0%
ATGGTTTCATTATTACCTCA+TGG + chr2.1:9752827-9752846 MS.gene54858:intron 30.0%
ATGTTCTTAATTAGACGAAG+TGG + chr2.1:9753119-9753138 MS.gene54858:intron 30.0%
ATTACCAACAATAAACCATG+AGG - chr2.1:9752845-9752864 None:intergenic 30.0%
ATTACCTCATGGTTTATTGT+TGG + chr2.1:9752838-9752857 MS.gene54858:intron 30.0%
GCATGATTTGAATTAATACC+TGG - chr2.1:9752972-9752991 None:intergenic 30.0%
TCAAGTATGAATCATGATTC+TGG + chr2.1:9752391-9752410 MS.gene54858:CDS 30.0%
TCTAAGCAAGAACTTCTAAA+TGG - chr2.1:9753071-9753090 None:intergenic 30.0%
TTCCAAGAAAAGAATGGTAA+AGG + chr2.1:9752342-9752361 MS.gene54858:CDS 30.0%
TTGCTATAACATTCCAATCA+GGG - chr2.1:9753259-9753278 None:intergenic 30.0%
TTTCTCGTTTGAAGAAAAAG+TGG + chr2.1:9753485-9753504 MS.gene54858:intron 30.0%
! GTGTATTGAGACAATTTTAG+GGG - chr2.1:9753217-9753236 None:intergenic 30.0%
! GTTGCATAACTTTTCTTGTA+GGG + chr2.1:9752883-9752902 MS.gene54858:intron 30.0%
! TTTGCTATAACATTCCAATC+AGG - chr2.1:9753260-9753279 None:intergenic 30.0%
! TTTTCTTGTAGGGTTTTCTA+TGG + chr2.1:9752893-9752912 MS.gene54858:intron 30.0%
AAAGTTCTACTCAGAAGTCA+TGG + chr2.1:9752225-9752244 MS.gene54858:CDS 35.0%
AAGAGCTTGCATTATTGCAT+GGG + chr2.1:9753557-9753576 MS.gene54858:CDS 35.0%
AAGCAAGAACTTCTAAATGG+TGG - chr2.1:9753068-9753087 None:intergenic 35.0%
AGCAAGAACTTCTAAATGGT+GGG - chr2.1:9753067-9753086 None:intergenic 35.0%
ATGATTCATACTTGAGTGCT+CGG - chr2.1:9752386-9752405 None:intergenic 35.0%
ATTGACCTATAGGCAATACA+GGG - chr2.1:9752282-9752301 None:intergenic 35.0%
GAACGTTGAAATGACTTCTT+AGG - chr2.1:9752325-9752344 None:intergenic 35.0%
GCATTAGCATATTGACCTAT+AGG - chr2.1:9752292-9752311 None:intergenic 35.0%
GGGAACAATGAAAATGGAAA+TGG + chr2.1:9753577-9753596 MS.gene54858:CDS 35.0%
GGTTAATACAAAGAAGAGAG+AGG + chr2.1:9753397-9753416 MS.gene54858:CDS 35.0%
GTACTATGAGAACAATCTTC+AGG - chr2.1:9752933-9752952 None:intergenic 35.0%
GTTTGTATCTTTGTGTCTCT+TGG + chr2.1:9752800-9752819 MS.gene54858:intron 35.0%
TATACAGAGAAGTTCCCATT+CGG - chr2.1:9753653-9753672 None:intergenic 35.0%
TATTGACCTATAGGCAATAC+AGG - chr2.1:9752283-9752302 None:intergenic 35.0%
TCAACGTTCCAAGAAAAGAA+TGG + chr2.1:9752336-9752355 MS.gene54858:CDS 35.0%
TTCAGCTGAATCAACCAATA+CGG - chr2.1:9752731-9752750 None:intergenic 35.0%
TTGCATGGGAACAATGAAAA+TGG + chr2.1:9753571-9753590 MS.gene54858:CDS 35.0%
TTTGTATTAACCGTTGCCTT+GGG - chr2.1:9753389-9753408 None:intergenic 35.0%
! AGCCTTTACCATTCTTTTCT+TGG - chr2.1:9752347-9752366 None:intergenic 35.0%
! CGTTGCATAACTTTTCTTGT+AGG + chr2.1:9752882-9752901 MS.gene54858:intron 35.0%
AAATGGAAATGGTGATTGCG+TGG + chr2.1:9753588-9753607 MS.gene54858:CDS 40.0%
AACTAACTGAAGTTCGTCTC+TGG - chr2.1:9753366-9753385 None:intergenic 40.0%
ATACAGAGAAGTTCCCATTC+GGG - chr2.1:9753652-9753671 None:intergenic 40.0%
CGATCAAAAACAGATGGATG+CGG + chr2.1:9752553-9752572 MS.gene54858:CDS 40.0%
CTTTGTATTAACCGTTGCCT+TGG - chr2.1:9753390-9753409 None:intergenic 40.0%
CTTTGTGTCTCTTGGACATA+TGG + chr2.1:9752808-9752827 MS.gene54858:intron 40.0%
GAAGAGCTTGCATTATTGCA+TGG + chr2.1:9753556-9753575 MS.gene54858:CDS 40.0%
GACATACTTGAAGATGCACA+TGG + chr2.1:9752778-9752797 MS.gene54858:CDS 40.0%
TCAGCTGAATCAACCAATAC+GGG - chr2.1:9752730-9752749 None:intergenic 40.0%
TTGACCTATAGGCAATACAG+GGG - chr2.1:9752281-9752300 None:intergenic 40.0%
!!! GTCTCGTGGTTTTATCATCT+TGG - chr2.1:9753301-9753320 None:intergenic 40.0%
ACAATCTGCTACAGCTGCAA+AGG - chr2.1:9752662-9752681 None:intergenic 45.0%
ATGATGTCTCTGAACCCGAA+TGG + chr2.1:9753635-9753654 MS.gene54858:CDS 45.0%
CGAACTTCAGTTAGTTCCCA+AGG + chr2.1:9753370-9753389 MS.gene54858:CDS 45.0%
GAGTTTATGTCCAACTTCGC+AGG + chr2.1:9753612-9753631 MS.gene54858:CDS 45.0%
GGCGTGCAAAACAACATGTT+CGG + chr2.1:9752443-9752462 MS.gene54858:CDS 45.0%
GGGAACTTCTCTGTATACCA+CGG + chr2.1:9753656-9753675 MS.gene54858:CDS 45.0%
GTCGCTCGATCAAAAACAGA+TGG + chr2.1:9752547-9752566 MS.gene54858:CDS 45.0%
TCAGTTAGTTCCCAAGGCAA+CGG + chr2.1:9753376-9753395 MS.gene54858:CDS 45.0%
TGATGTCTCTGAACCCGAAT+GGG + chr2.1:9753636-9753655 MS.gene54858:CDS 45.0%
! CAGATTGTCGGAGAAAGTGT+TGG + chr2.1:9752202-9752221 MS.gene54858:CDS 45.0%
! TTTTGATCGAGCGACTTCAC+TGG - chr2.1:9752542-9752561 None:intergenic 45.0%
!! GCAAGTACTGAACAAAACCG+CGG + chr2.1:9752700-9752719 MS.gene54858:CDS 45.0%
AAACCGCGGTATTCCCGTAT+TGG + chr2.1:9752714-9752733 MS.gene54858:CDS 50.0%
ACAATCGCTGTGTCTCATCC+AGG + chr2.1:9752951-9752970 MS.gene54858:CDS 50.0%
ACAGGGGTAGCACATGAAGA+TGG - chr2.1:9752265-9752284 None:intergenic 50.0%
AGACAACGACTTGAAGCTCG+AGG + chr2.1:9753322-9753341 MS.gene54858:CDS 50.0%
CAACCAATACGGGAATACCG+CGG - chr2.1:9752720-9752739 None:intergenic 50.0%
CAAGTCGTTGTCTTGTCTCG+TGG - chr2.1:9753315-9753334 None:intergenic 50.0%
GAGACATCATCCTGCGAAGT+TGG - chr2.1:9753625-9753644 None:intergenic 50.0%
GCTACCCCTGTATTGCCTAT+AGG + chr2.1:9752274-9752293 MS.gene54858:CDS 50.0%
GCTCGAGGAAGTTTGTGTCA+TGG + chr2.1:9753337-9753356 MS.gene54858:CDS 50.0%
GGGGTAGCACATGAAGATGG+AGG - chr2.1:9752262-9752281 None:intergenic 55.0%
TGAGTGCTCGGTTGATCGCT+TGG - chr2.1:9752374-9752393 None:intergenic 55.0%
! GCGACTTCACTGGCTGTCTT+CGG - chr2.1:9752532-9752551 None:intergenic 55.0%
! TCACTGGCTGTCTTCGGTGC+AGG - chr2.1:9752526-9752545 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 9752199 9753681 9752199 ID=MS.gene54858
chr2.1 mRNA 9752199 9753681 9752199 ID=MS.gene54858.t1;Parent=MS.gene54858
chr2.1 exon 9752199 9752799 9752199 ID=MS.gene54858.t1.exon1;Parent=MS.gene54858.t1
chr2.1 CDS 9752199 9752799 9752199 ID=cds.MS.gene54858.t1;Parent=MS.gene54858.t1
chr2.1 exon 9752904 9752972 9752904 ID=MS.gene54858.t1.exon2;Parent=MS.gene54858.t1
chr2.1 CDS 9752904 9752972 9752904 ID=cds.MS.gene54858.t1;Parent=MS.gene54858.t1
chr2.1 exon 9753276 9753418 9753276 ID=MS.gene54858.t1.exon3;Parent=MS.gene54858.t1
chr2.1 CDS 9753276 9753418 9753276 ID=cds.MS.gene54858.t1;Parent=MS.gene54858.t1
chr2.1 exon 9753499 9753681 9753499 ID=MS.gene54858.t1.exon4;Parent=MS.gene54858.t1
chr2.1 CDS 9753499 9753681 9753499 ID=cds.MS.gene54858.t1;Parent=MS.gene54858.t1
Gene Sequence

>MS.gene54858

ATGCAGATTGTCGGAGAAAGTGTTGGAAAGTTCTACTCAGAAGTCATGGAAGATTTACTTCCTCCATCTTCATGTGCTACCCCTGTATTGCCTATAGGTCAATATGCTAATGCTACTGCTATCCCTAAGAAGTCATTTCAACGTTCCAAGAAAAGAATGGTAAAGGCTAATACCAAGCGATCAACCGAGCACTCAAGTATGAATCATGATTCTGGCAATTCTATCAAAAAGAATACTTTTATTTGGCGTGCAAAACAACATGTTCGGAGTGCAGATATTAAATTGAATATTAGCAGTGATGAAAATCTACAAAATACGAAGATTCCTGCACCGAAGACAGCCAGTGAAGTCGCTCGATCAAAAACAGATGGATGCGGTTCATCACAATCATGTGAACTCTCCAATGTAAATGAAAATCATGAGGCTACTGTTTCAAAGACTGCTTCTGCAGAAGTAACAACCTTTGCAGCTGTAGCAGATTGTTGCAATGAAACTGAAAAAGCAAGTACTGAACAAAACCGCGGTATTCCCGTATTGGTTGATTCAGCTGAAGAGAAAAATATGAGTTCTATCTCAAGTGACATACTTGAAGATGCACATGGTTTGTATCTTTGTGTCTCTTGGACATATGGTTTCATTATTACCTCATGGTTTATTGTTGGTAATTTTTTGTTTCTGATAATCGTTGCATAACTTTTCTTGTAGGGTTTTCTATGGTCAAAGCTATGCAGCCTGAAGATTGTTCTCATAGTACAATCGCTGTGTCTCATCCAGGTATTAATTCAAATCATGCTGAAGTACTAATTGAATTTAGGTAACATTTTTCAAATCAATATGAAGTGCTTTCTCAATTTAAGTATTATAACCCACCATTTAGAAGTTCTTGCTTAGAATTTGGAGAAAATGTTTTCTGTACTGCCATGTTCTTAATTAGACGAAGTGGAATCTGTTTTTTTGTTAAACAACTAAACTGTTTTTAGTATAATATGCAGATATAAACTTTAGTTTATTCATGCCCCTAAAATTGTCTCAATACACATAACAATATTCATTTTAACCCTGATTGGAATGTTATAGCAAATATTGATGCTTCTATAGACCAAGATGATAAAACCACGAGACAAGACAACGACTTGAAGCTCGAGGAAGTTTGTGTCATGGTTACCAGAGACGAACTTCAGTTAGTTCCCAAGGCAACGGTTAATACAAAGAAGAGAGAGGTAAACACTCATTCCCCTTTTCTATTTTTTATGCACGTTGTTAAACTTAAAGTAATTTTTTCTTTATTTCTCGTTTGAAGAAAAAGTGGCTGCAACCCTTTTCTTTGTATAAGAAATCTACAAGGAAGCAAGAGTATGAAGAGCTTGCATTATTGCATGGGAACAATGAAAATGGAAATGGTGATTGCGTGGAGAGTTTATGTCCAACTTCGCAGGATGATGTCTCTGAACCCGAATGGGAACTTCTCTGTATACCACGGTGA

Protein sequence

>MS.gene54858.t1

MQIVGESVGKFYSEVMEDLLPPSSCATPVLPIGQYANATAIPKKSFQRSKKRMVKANTKRSTEHSSMNHDSGNSIKKNTFIWRAKQHVRSADIKLNISSDENLQNTKIPAPKTASEVARSKTDGCGSSQSCELSNVNENHEATVSKTASAEVTTFAAVADCCNETEKASTEQNRGIPVLVDSAEEKNMSSISSDILEDAHGFSMVKAMQPEDCSHSTIAVSHPANIDASIDQDDKTTRQDNDLKLEEVCVMVTRDELQLVPKATVNTKKREKKWLQPFSLYKKSTRKQEYEELALLHGNNENGNGDCVESLCPTSQDDVSEPEWELLCIPR