AlfalfaGEDB | MS.gene57950

M. sativa cultivar XinJiangDaYe / MS.gene57950

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57950.t1	XP_003610246.2	94.5	181	6	2	1	181	1	177	1.00E-79	306.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57950.t1	Q9M0B9	38.6	184	105	5	1	180	1	180	6.0e-17	89.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57950.t1	G7JEC9	94.5	181	6	2	1	181	1	177	7.2e-80	306.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049149	MS.gene57950	0.800545	1.37E-48	-1.69E-46
MS.gene049230	MS.gene57950	0.822147	2.77E-53	-1.69E-46
MS.gene049378	MS.gene57950	0.848506	5.87E-60	-1.69E-46
MS.gene049577	MS.gene57950	0.810502	1.12E-50	-1.69E-46
MS.gene049910	MS.gene57950	0.835337	1.78E-56	-1.69E-46
MS.gene050226	MS.gene57950	0.830935	2.22E-55	-1.69E-46
MS.gene050227	MS.gene57950	0.832601	8.61E-56	-1.69E-46
MS.gene050228	MS.gene57950	0.832848	7.48E-56	-1.69E-46
MS.gene050426	MS.gene57950	-0.800999	1.11E-48	-1.69E-46
MS.gene050623	MS.gene57950	-0.817706	2.87E-52	-1.69E-46
MS.gene050642	MS.gene57950	0.850045	2.19E-60	-1.69E-46
MS.gene050820	MS.gene57950	0.812219	4.73E-51	-1.69E-46
MS.gene050953	MS.gene57950	0.800998	1.11E-48	-1.69E-46
MS.gene05119	MS.gene57950	-0.81796	2.51E-52	-1.69E-46
MS.gene051373	MS.gene57950	0.820073	8.31E-53	-1.69E-46
MS.gene05137	MS.gene57950	0.820099	8.20E-53	-1.69E-46
MS.gene051983	MS.gene57950	0.868456	6.44E-66	-1.69E-46
MS.gene052077	MS.gene57950	0.856652	2.78E-62	-1.69E-46
MS.gene052176	MS.gene57950	0.818082	2.36E-52	-1.69E-46
MS.gene052309	MS.gene57950	-0.840994	6.22E-58	-1.69E-46
MS.gene052340	MS.gene57950	0.813412	2.59E-51	-1.69E-46
MS.gene052426	MS.gene57950	0.805	1.65E-49	-1.69E-46
MS.gene053494	MS.gene57950	0.805088	1.58E-49	-1.69E-46
MS.gene053836	MS.gene57950	0.846231	2.47E-59	-1.69E-46
MS.gene054930	MS.gene57950	0.874267	7.72E-68	-1.69E-46
MS.gene054983	MS.gene57950	0.841121	5.76E-58	-1.69E-46
MS.gene055367	MS.gene57950	0.821265	4.43E-53	-1.69E-46
MS.gene05553	MS.gene57950	0.833893	4.11E-56	-1.69E-46
MS.gene055701	MS.gene57950	0.854459	1.21E-61	-1.69E-46
MS.gene056723	MS.gene57950	0.809939	1.48E-50	-1.69E-46
MS.gene056979	MS.gene57950	0.858174	9.87E-63	-1.69E-46
MS.gene05761	MS.gene57950	0.808333	3.26E-50	-1.69E-46
MS.gene058002	MS.gene57950	0.8001	1.69E-48	-1.69E-46
MS.gene058142	MS.gene57950	0.826928	2.07E-54	-1.69E-46
MS.gene058435	MS.gene57950	0.834653	2.65E-56	-1.69E-46
MS.gene058710	MS.gene57950	0.803719	3.05E-49	-1.69E-46
MS.gene058749	MS.gene57950	0.809369	1.96E-50	-1.69E-46
MS.gene059285	MS.gene57950	0.84187	3.66E-58	-1.69E-46
MS.gene059511	MS.gene57950	0.831437	1.67E-55	-1.69E-46
MS.gene05984	MS.gene57950	0.805741	1.15E-49	-1.69E-46
MS.gene059894	MS.gene57950	0.818443	1.96E-52	-1.69E-46
MS.gene059899	MS.gene57950	0.81784	2.68E-52	-1.69E-46
MS.gene061049	MS.gene57950	0.828313	9.64E-55	-1.69E-46
MS.gene061114	MS.gene57950	-0.827794	1.29E-54	-1.69E-46
MS.gene061159	MS.gene57950	0.824698	7.01E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57950.t1	MTR_4g129560	94.475	181	6	2	1	181	1	177	4.01e-118	332

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene57950.t1	AT4G30410	42.391	184	98	5	1	180	1	180	1.40e-30	110
MS.gene57950.t1	AT4G30410	42.391	184	98	5	1	180	1	180	1.40e-30	110
MS.gene57950.t1	AT5G57780	38.764	178	85	6	1	177	2	156	1.54e-28	104

Find 25 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTATTCTCTTTATAGAAAA+AGG	0.151336	8.4:+5261701	MS.gene57950:CDS
TAAATCAGCAGAAAGCTTTA+TGG	0.212678	8.4:-5261536	None:intergenic
TCTTCACATAAGAAGGTAAT+AGG	0.223742	8.4:+5261661	MS.gene57950:CDS
TTGTTGAAGTATTTGCAATT+AGG	0.256685	8.4:-5261606	None:intergenic
TGAGAGATTCTATATAATCT+AGG	0.292570	8.4:-5261882	None:intergenic
TGTTGAAGTATTTGCAATTA+GGG	0.303984	8.4:-5261605	None:intergenic
ATTCCTAAGGAAATGGATAA+AGG	0.331543	8.4:+5261453	MS.gene57950:CDS
GTTACCAGGTGGAAAATTCA+TGG	0.380790	8.4:+5261834	MS.gene57950:CDS
AGCAAGAATTCCTAAGGAAA+TGG	0.389199	8.4:+5261446	MS.gene57950:CDS
CTTCACATAAGAAGGTAATA+GGG	0.431570	8.4:+5261662	MS.gene57950:CDS
TTCCTAAGGAAATGGATAAA+GGG	0.440057	8.4:+5261454	MS.gene57950:CDS
CTAAAGACAAAACTACCCTT+TGG	0.442557	8.4:+5261575	MS.gene57950:CDS
GACCCTTTATCCATTTCCTT+AGG	0.455134	8.4:-5261456	None:intergenic
GAGAGATTCTATATAATCTA+GGG	0.474006	8.4:-5261881	None:intergenic
GTCTTTAGACGTGGAAGCCA+TGG	0.491623	8.4:-5261560	None:intergenic
AACCTCATCTTCACATAAGA+AGG	0.491845	8.4:+5261654	MS.gene57950:CDS
TGCTCAAGCAAGAATTCCTA+AGG	0.547032	8.4:+5261440	MS.gene57950:CDS
TACCTTCTTATGTGAAGATG+AGG	0.560965	8.4:-5261656	None:intergenic
GCTTTCTGCTGATTTAGCCA+TGG	0.566451	8.4:+5261543	MS.gene57950:CDS
CTCAAGAGTTTGTTACCAGG+TGG	0.612619	8.4:+5261823	MS.gene57950:CDS
AGGCTCAAGAGTTTGTTACC+AGG	0.621754	8.4:+5261820	MS.gene57950:CDS
TGGTTAAGAAAAGAACAAGA+AGG	0.644687	8.4:+5261800	MS.gene57950:CDS
TCGATTAACGAAGAGTTCTG+AGG	0.651928	8.4:-5261941	None:intergenic
GTTGAGAAGAAATCGTGCAA+AGG	0.662740	8.4:+5261744	MS.gene57950:CDS
TCGTGCAAAGGAAAGAGTTG+AGG	0.666359	8.4:+5261756	MS.gene57950:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CTTTATAGAAAAAGGATAAT+TGG	+	chr8.4:5261709-5261728	MS.gene57950:CDS	20.0%
!	ATAGAAAAAGGATAATTGGA+AGG	+	chr8.4:5261713-5261732	MS.gene57950:CDS	25.0%
!	CCTATTCTCTTTATAGAAAA+AGG	+	chr8.4:5261701-5261720	MS.gene57950:CDS	25.0%
!	GAGAGATTCTATATAATCTA+GGG	-	chr8.4:5261884-5261903	None:intergenic	25.0%
!	TGAGAGATTCTATATAATCT+AGG	-	chr8.4:5261885-5261904	None:intergenic	25.0%
!!	CCTTTTTCTATAAAGAGAAT+AGG	-	chr8.4:5261704-5261723	None:intergenic	25.0%
!!	TGTTGAAGTATTTGCAATTA+GGG	-	chr8.4:5261608-5261627	None:intergenic	25.0%
!!	TTGTTGAAGTATTTGCAATT+AGG	-	chr8.4:5261609-5261628	None:intergenic	25.0%
	ATTCCTAAGGAAATGGATAA+AGG	+	chr8.4:5261453-5261472	MS.gene57950:CDS	30.0%
	CTTCACATAAGAAGGTAATA+GGG	+	chr8.4:5261662-5261681	MS.gene57950:CDS	30.0%
	TAAATCAGCAGAAAGCTTTA+TGG	-	chr8.4:5261539-5261558	None:intergenic	30.0%
	TCTTCACATAAGAAGGTAAT+AGG	+	chr8.4:5261661-5261680	MS.gene57950:CDS	30.0%
	TGGTTAAGAAAAGAACAAGA+AGG	+	chr8.4:5261800-5261819	MS.gene57950:CDS	30.0%
	TTCCTAAGGAAATGGATAAA+GGG	+	chr8.4:5261454-5261473	MS.gene57950:CDS	30.0%
	AACCTCATCTTCACATAAGA+AGG	+	chr8.4:5261654-5261673	MS.gene57950:CDS	35.0%
	AGCAAGAATTCCTAAGGAAA+TGG	+	chr8.4:5261446-5261465	MS.gene57950:CDS	35.0%
	CTAAAGACAAAACTACCCTT+TGG	+	chr8.4:5261575-5261594	MS.gene57950:CDS	35.0%
	TACCTTCTTATGTGAAGATG+AGG	-	chr8.4:5261659-5261678	None:intergenic	35.0%
!	AATTAGGGCTTTTCTCCAAA+GGG	-	chr8.4:5261593-5261612	None:intergenic	35.0%
	GACCCTTTATCCATTTCCTT+AGG	-	chr8.4:5261459-5261478	None:intergenic	40.0%
	GTTACCAGGTGGAAAATTCA+TGG	+	chr8.4:5261834-5261853	MS.gene57950:CDS	40.0%
	GTTGAGAAGAAATCGTGCAA+AGG	+	chr8.4:5261744-5261763	MS.gene57950:CDS	40.0%
	TCGATTAACGAAGAGTTCTG+AGG	-	chr8.4:5261944-5261963	None:intergenic	40.0%
	TGCTCAAGCAAGAATTCCTA+AGG	+	chr8.4:5261440-5261459	MS.gene57950:CDS	40.0%
!	CAATTAGGGCTTTTCTCCAA+AGG	-	chr8.4:5261594-5261613	None:intergenic	40.0%
!	TCATCCATGAATTTTCCACC+TGG	-	chr8.4:5261841-5261860	None:intergenic	40.0%
!!!	GGTAGTTTTGTCTTTAGACG+TGG	-	chr8.4:5261572-5261591	None:intergenic	40.0%
	TCGTGCAAAGGAAAGAGTTG+AGG	+	chr8.4:5261756-5261775	MS.gene57950:CDS	45.0%
!!	AGGCGAGTTTTATAGCGACA+CGG	+	chr8.4:5261776-5261795	MS.gene57950:CDS	45.0%
!!	AGGCTCAAGAGTTTGTTACC+AGG	+	chr8.4:5261820-5261839	MS.gene57950:CDS	45.0%
!!	CTCAAGAGTTTGTTACCAGG+TGG	+	chr8.4:5261823-5261842	MS.gene57950:CDS	45.0%
!!	GAGTTTTATAGCGACACGGT+TGG	+	chr8.4:5261780-5261799	MS.gene57950:CDS	45.0%
!!	GCTTTCTGCTGATTTAGCCA+TGG	+	chr8.4:5261543-5261562	MS.gene57950:CDS	45.0%
	GTCTTTAGACGTGGAAGCCA+TGG	-	chr8.4:5261563-5261582	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	5261424	5261969	5261424	ID=MS.gene57950
chr8.4	mRNA	5261424	5261969	5261424	ID=MS.gene57950.t1;Parent=MS.gene57950
chr8.4	exon	5261424	5261969	5261424	ID=MS.gene57950.t1.exon1;Parent=MS.gene57950.t1
chr8.4	CDS	5261424	5261969	5261424	ID=cds.MS.gene57950.t1;Parent=MS.gene57950.t1

Gene Sequence

>MS.gene57950

ATGCGAAATTTGAGCTTGCTCAAGCAAGAATTCCTAAGGAAATGGATAAAGGGTCTAAAAAAATATTATAACTCTCAAAAGAAGAATATGAACTTGTTCGAGAGAAAGAAAGCCATAAAGCTTTCTGCTGATTTAGCCATGGCTTCCACGTCTAAAGACAAAACTACCCTTTGGAGAAAAGCCCTAATTGCAAATACTTCAACAAGCATAGATGAACAAGACATATCAACAACCTCATCTTCACATAAGAAGGTAATAGGGAAAAACTCGACGAATTCCTATTCTCTTTATAGAAAAAGGATAATTGGAAGGAGAAAGACGTTGAGAAGAAATCGTGCAAAGGAAAGAGTTGAGGCGAGTTTTATAGCGACACGGTTGGTTAAGAAAAGAACAAGAAGGCTCAAGAGTTTGTTACCAGGTGGAAAATTCATGGATGATGCTTGTTTAGTTGATGAAACCCTAGATTATATAGAATCTCTCAAAGCTCAAGTTGAAGTGATGAGATGTCTTGTTACTGCCTCAGAACTCTTCGTTAATCGACCGTAA

Protein sequence

>MS.gene57950.t1

MRNLSLLKQEFLRKWIKGLKKYYNSQKKNMNLFERKKAIKLSADLAMASTSKDKTTLWRKALIANTSTSIDEQDISTTSSSHKKVIGKNSTNSYSLYRKRIIGRRKTLRRNRAKERVEASFIATRLVKKRTRRLKSLLPGGKFMDDACLVDETLDYIESLKAQVEVMRCLVTASELFVNRP