Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61492.t1 | XP_003609290.1 | 95.9 | 170 | 4 | 2 | 1 | 169 | 1 | 168 | 4.20E-88 | 334 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61492.t1 | G7JCV5 | 95.9 | 170 | 4 | 2 | 1 | 169 | 1 | 168 | 3.0e-88 | 334.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene61492 | -0.80131 | 9.57E-49 | -1.69E-46 |
| MS.gene049217 | MS.gene61492 | -0.832872 | 7.38E-56 | -1.69E-46 |
| MS.gene049222 | MS.gene61492 | -0.807709 | 4.43E-50 | -1.69E-46 |
| MS.gene049285 | MS.gene61492 | -0.805746 | 1.15E-49 | -1.69E-46 |
| MS.gene049627 | MS.gene61492 | -0.819686 | 1.02E-52 | -1.69E-46 |
| MS.gene050381 | MS.gene61492 | 0.834073 | 3.70E-56 | -1.69E-46 |
| MS.gene050504 | MS.gene61492 | -0.821416 | 4.09E-53 | -1.69E-46 |
| MS.gene050868 | MS.gene61492 | -0.82665 | 2.42E-54 | -1.69E-46 |
| MS.gene050871 | MS.gene61492 | -0.82797 | 1.17E-54 | -1.69E-46 |
| MS.gene051694 | MS.gene61492 | -0.828381 | 9.28E-55 | -1.69E-46 |
| MS.gene051858 | MS.gene61492 | -0.823007 | 1.75E-53 | -1.69E-46 |
| MS.gene051946 | MS.gene61492 | 0.831525 | 1.59E-55 | -1.69E-46 |
| MS.gene052164 | MS.gene61492 | -0.809952 | 1.47E-50 | -1.69E-46 |
| MS.gene05234 | MS.gene61492 | 0.810005 | 1.43E-50 | -1.69E-46 |
| MS.gene052482 | MS.gene61492 | -0.807143 | 5.84E-50 | -1.69E-46 |
| MS.gene052486 | MS.gene61492 | -0.818992 | 1.47E-52 | -1.69E-46 |
| MS.gene052966 | MS.gene61492 | -0.812965 | 3.25E-51 | -1.69E-46 |
| MS.gene053203 | MS.gene61492 | -0.801448 | 8.96E-49 | -1.69E-46 |
| MS.gene053420 | MS.gene61492 | 0.823374 | 1.43E-53 | -1.69E-46 |
| MS.gene053824 | MS.gene61492 | -0.811205 | 7.86E-51 | -1.69E-46 |
| MS.gene054003 | MS.gene61492 | -0.805901 | 1.07E-49 | -1.69E-46 |
| MS.gene054595 | MS.gene61492 | -0.814415 | 1.56E-51 | -1.69E-46 |
| MS.gene055154 | MS.gene61492 | 0.806586 | 7.65E-50 | -1.69E-46 |
| MS.gene055202 | MS.gene61492 | 0.821232 | 4.51E-53 | -1.69E-46 |
| MS.gene055362 | MS.gene61492 | -0.801774 | 7.68E-49 | -1.69E-46 |
| MS.gene055502 | MS.gene61492 | -0.8218 | 3.33E-53 | -1.69E-46 |
| MS.gene055577 | MS.gene61492 | -0.8406 | 7.89E-58 | -1.69E-46 |
| MS.gene055941 | MS.gene61492 | -0.803772 | 2.97E-49 | -1.69E-46 |
| MS.gene056409 | MS.gene61492 | -0.804875 | 1.75E-49 | -1.69E-46 |
| MS.gene056504 | MS.gene61492 | -0.801121 | 1.05E-48 | -1.69E-46 |
| MS.gene056550 | MS.gene61492 | 0.808011 | 3.82E-50 | -1.69E-46 |
| MS.gene056610 | MS.gene61492 | -0.802562 | 5.29E-49 | -1.69E-46 |
| MS.gene057014 | MS.gene61492 | 0.821778 | 3.37E-53 | -1.69E-46 |
| MS.gene057857 | MS.gene61492 | -0.805625 | 1.22E-49 | -1.69E-46 |
| MS.gene058157 | MS.gene61492 | -0.804993 | 1.65E-49 | -1.69E-46 |
| MS.gene05837 | MS.gene61492 | -0.817919 | 2.57E-52 | -1.69E-46 |
| MS.gene058419 | MS.gene61492 | -0.800128 | 1.67E-48 | -1.69E-46 |
| MS.gene058773 | MS.gene61492 | -0.827691 | 1.36E-54 | -1.69E-46 |
| MS.gene059022 | MS.gene61492 | -0.811718 | 6.08E-51 | -1.69E-46 |
| MS.gene059023 | MS.gene61492 | -0.823384 | 1.43E-53 | -1.69E-46 |
| MS.gene059223 | MS.gene61492 | 0.808794 | 2.60E-50 | -1.69E-46 |
| MS.gene059718 | MS.gene61492 | -0.822118 | 2.81E-53 | -1.69E-46 |
| MS.gene059902 | MS.gene61492 | -0.824082 | 9.78E-54 | -1.69E-46 |
| MS.gene060595 | MS.gene61492 | 0.814082 | 1.85E-51 | -1.69E-46 |
| MS.gene060762 | MS.gene61492 | -0.814225 | 1.72E-51 | -1.69E-46 |
| MS.gene06077 | MS.gene61492 | -0.822097 | 2.84E-53 | -1.69E-46 |
| MS.gene06092 | MS.gene61492 | -0.804074 | 2.57E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61492.t1 | MTR_4g114080 | 95.882 | 170 | 4 | 2 | 1 | 169 | 1 | 168 | 9.83e-118 | 330 |
| MS.gene61492.t1 | MTR_4g011020 | 67.308 | 156 | 49 | 1 | 1 | 156 | 5 | 158 | 1.11e-77 | 229 |
| MS.gene61492.t1 | MTR_2g103490 | 61.538 | 169 | 60 | 3 | 1 | 168 | 5 | 169 | 1.82e-68 | 205 |
| MS.gene61492.t1 | MTR_2g103460 | 60.241 | 166 | 61 | 3 | 1 | 165 | 5 | 166 | 3.50e-65 | 197 |
| MS.gene61492.t1 | MTR_2g005880 | 61.151 | 139 | 52 | 1 | 4 | 142 | 2 | 138 | 1.15e-55 | 173 |
| MS.gene61492.t1 | MTR_5g021610 | 54.730 | 148 | 61 | 2 | 2 | 145 | 22 | 167 | 1.05e-53 | 169 |
| MS.gene61492.t1 | MTR_8g076160 | 54.730 | 148 | 57 | 3 | 4 | 145 | 12 | 155 | 6.68e-52 | 164 |
| MS.gene61492.t1 | MTR_1g115170 | 55.405 | 148 | 61 | 3 | 20 | 166 | 1 | 144 | 3.94e-50 | 158 |
| MS.gene61492.t1 | MTR_0003s0630 | 52.206 | 136 | 61 | 2 | 3 | 134 | 32 | 167 | 3.86e-48 | 155 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61492.t1 | AT1G80160 | 65.644 | 163 | 54 | 1 | 2 | 164 | 6 | 166 | 2.05e-76 | 225 |
| MS.gene61492.t1 | AT1G80160 | 65.644 | 163 | 54 | 1 | 2 | 164 | 47 | 207 | 5.47e-76 | 226 |
| MS.gene61492.t1 | AT1G15380 | 62.722 | 169 | 58 | 3 | 2 | 167 | 6 | 172 | 4.81e-72 | 214 |
| MS.gene61492.t1 | AT1G15380 | 62.722 | 169 | 58 | 3 | 2 | 167 | 6 | 172 | 4.81e-72 | 214 |
| MS.gene61492.t1 | AT2G28420 | 53.077 | 130 | 59 | 2 | 7 | 134 | 18 | 147 | 2.90e-50 | 160 |
| MS.gene61492.t1 | AT1G80160 | 64.655 | 116 | 39 | 1 | 49 | 164 | 27 | 140 | 2.71e-48 | 153 |
Find 35 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACATTCTTGGTCATACTTTC+TGG | 0.185353 | 8.3:-13314001 | None:intergenic |
| TTGATTAGGTTGTTTAATTA+TGG | 0.210559 | 8.3:+13313947 | MS.gene61492:intron |
| TGGATTAATGTGCACATTCT+TGG | 0.286505 | 8.3:-13314014 | None:intergenic |
| AAGAACAGAGTAGAGGAAAA+TGG | 0.294782 | 8.3:+13314236 | MS.gene61492:CDS |
| CATTCTTGGTCATACTTTCT+GGG | 0.322846 | 8.3:-13314000 | None:intergenic |
| ATCATTGAATCACATTTCAT+TGG | 0.367034 | 8.3:+13313677 | MS.gene61492:CDS |
| TACTTTCTGGGTCATCTGAT+TGG | 0.372059 | 8.3:-13313988 | None:intergenic |
| TTCTCTGATAGAGGTACAAC+TGG | 0.377022 | 8.3:-13314338 | None:intergenic |
| AAGGAGGTAGGCCTCTTAAT+AGG | 0.393251 | 8.3:-13313756 | None:intergenic |
| TTGGCTTCTTTCCTATTAAG+AGG | 0.414495 | 8.3:+13313745 | MS.gene61492:CDS |
| AAAGAAATGTGGTTGTCCTT+TGG | 0.424055 | 8.3:-13314034 | None:intergenic |
| ACAATTGCAGATTTCAATCA+TGG | 0.433463 | 8.3:-13314300 | None:intergenic |
| ATTTCAATCATGGTGCCATC+TGG | 0.434106 | 8.3:-13314290 | None:intergenic |
| CTCTATCAGAGAACACAATT+TGG | 0.449396 | 8.3:+13314348 | MS.gene61492:CDS |
| TACATACCATGCACCATTGA+AGG | 0.456374 | 8.3:-13313781 | None:intergenic |
| GATAGAGGTACAACTGGGTC+TGG | 0.465555 | 8.3:-13314332 | None:intergenic |
| CAAATTGTGTTCTCTGATAG+AGG | 0.467908 | 8.3:-13314347 | None:intergenic |
| CTGGGTCATCTGATTGGAGA+AGG | 0.480699 | 8.3:-13313982 | None:intergenic |
| TTGTTGCTAGTGTGAAAGTA+TGG | 0.480825 | 8.3:+13314169 | MS.gene61492:intron |
| CCTACCTCCTTAGCCTTCAA+TGG | 0.485564 | 8.3:+13313768 | MS.gene61492:CDS |
| TCATGGTGCCATCTGGATCA+TGG | 0.496370 | 8.3:-13314283 | None:intergenic |
| AGGTTGTTTAATTATGGCAT+TGG | 0.497038 | 8.3:+13313953 | MS.gene61492:intron |
| TTCAATGATTTGAGTTGAAG+GGG | 0.537052 | 8.3:-13313663 | None:intergenic |
| ATTCAATGATTTGAGTTGAA+GGG | 0.552122 | 8.3:-13313664 | None:intergenic |
| CCATGCACCATTGAAGGCTA+AGG | 0.566485 | 8.3:-13313775 | None:intergenic |
| GATTCAATGATTTGAGTTGA+AGG | 0.580881 | 8.3:-13313665 | None:intergenic |
| GTATGTGAAGAACAGAGTAG+AGG | 0.586484 | 8.3:+13314229 | MS.gene61492:CDS |
| CCATTGAAGGCTAAGGAGGT+AGG | 0.594731 | 8.3:-13313768 | None:intergenic |
| TTGTTCTTCCATGATCCAGA+TGG | 0.596741 | 8.3:+13314275 | MS.gene61492:CDS |
| GTGTGAAAGTATGGTAGCAG+TGG | 0.597656 | 8.3:+13314178 | MS.gene61492:intron |
| GATATACTTGGAAAGAAATG+TGG | 0.600017 | 8.3:-13314045 | None:intergenic |
| CCTTAGCCTTCAATGGTGCA+TGG | 0.603871 | 8.3:+13313775 | MS.gene61492:CDS |
| GAATGTGCACATTAATCCAA+AGG | 0.612046 | 8.3:+13314018 | MS.gene61492:CDS |
| TGCACCATTGAAGGCTAAGG+AGG | 0.624890 | 8.3:-13313772 | None:intergenic |
| TCTCTGATAGAGGTACAACT+GGG | 0.730796 | 8.3:-13314337 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTTACACAAATAAATAGA+GGG | - | chr8.3:13314078-13314097 | None:intergenic | 15.0% |
| !! | TAATTTACACAAATAAATAG+AGG | - | chr8.3:13314079-13314098 | None:intergenic | 15.0% |
| !!! | TATACAAGTTATTTTAACAA+TGG | - | chr8.3:13313864-13313883 | None:intergenic | 15.0% |
| !!! | ATTTTCCTTTTCATTGAATT+TGG | + | chr8.3:13313898-13313917 | MS.gene61492:intron | 20.0% |
| !!! | TTGATTAGGTTGTTTAATTA+TGG | + | chr8.3:13313947-13313966 | MS.gene61492:intron | 20.0% |
| ! | AGTATCCAAATTCAATGAAA+AGG | - | chr8.3:13313906-13313925 | None:intergenic | 25.0% |
| ! | ATCATTGAATCACATTTCAT+TGG | + | chr8.3:13313677-13313696 | MS.gene61492:CDS | 25.0% |
| !! | GATTTTTATGTCAATGTTCT+TGG | + | chr8.3:13313726-13313745 | MS.gene61492:CDS | 25.0% |
| !!! | ATTCAATGATTTGAGTTGAA+GGG | - | chr8.3:13313667-13313686 | None:intergenic | 25.0% |
| ACAATTGCAGATTTCAATCA+TGG | - | chr8.3:13314303-13314322 | None:intergenic | 30.0% | |
| AGGTTGTTTAATTATGGCAT+TGG | + | chr8.3:13313953-13313972 | MS.gene61492:intron | 30.0% | |
| GATATACTTGGAAAGAAATG+TGG | - | chr8.3:13314048-13314067 | None:intergenic | 30.0% | |
| !! | GATTCAATGATTTGAGTTGA+AGG | - | chr8.3:13313668-13313687 | None:intergenic | 30.0% |
| !! | TTCAATGATTTGAGTTGAAG+GGG | - | chr8.3:13313666-13313685 | None:intergenic | 30.0% |
| AAAGAAATGTGGTTGTCCTT+TGG | - | chr8.3:13314037-13314056 | None:intergenic | 35.0% | |
| AAGAACAGAGTAGAGGAAAA+TGG | + | chr8.3:13314236-13314255 | MS.gene61492:CDS | 35.0% | |
| ACATTCTTGGTCATACTTTC+TGG | - | chr8.3:13314004-13314023 | None:intergenic | 35.0% | |
| CAAATTGTGTTCTCTGATAG+AGG | - | chr8.3:13314350-13314369 | None:intergenic | 35.0% | |
| CATTCTTGGTCATACTTTCT+GGG | - | chr8.3:13314003-13314022 | None:intergenic | 35.0% | |
| CTCTATCAGAGAACACAATT+TGG | + | chr8.3:13314348-13314367 | MS.gene61492:CDS | 35.0% | |
| GAATGTGCACATTAATCCAA+AGG | + | chr8.3:13314018-13314037 | MS.gene61492:CDS | 35.0% | |
| TGGATTAATGTGCACATTCT+TGG | - | chr8.3:13314017-13314036 | None:intergenic | 35.0% | |
| TTGGCTTCTTTCCTATTAAG+AGG | + | chr8.3:13313745-13313764 | MS.gene61492:CDS | 35.0% | |
| TTGTTGCTAGTGTGAAAGTA+TGG | + | chr8.3:13314169-13314188 | MS.gene61492:intron | 35.0% | |
| ! | ACTAACTGCATGTGCATTTT+GGG | + | chr8.3:13313923-13313942 | MS.gene61492:intron | 35.0% |
| ! | TACTAACTGCATGTGCATTT+TGG | + | chr8.3:13313922-13313941 | MS.gene61492:intron | 35.0% |
| !!! | TGTGCATTTTGGGTTTGATT+AGG | + | chr8.3:13313933-13313952 | MS.gene61492:intron | 35.0% |
| GAGGGAGAGAATGATATACT+TGG | - | chr8.3:13314060-13314079 | None:intergenic | 40.0% | |
| GTATGTGAAGAACAGAGTAG+AGG | + | chr8.3:13314229-13314248 | MS.gene61492:CDS | 40.0% | |
| TACATACCATGCACCATTGA+AGG | - | chr8.3:13313784-13313803 | None:intergenic | 40.0% | |
| TACTTTCTGGGTCATCTGAT+TGG | - | chr8.3:13313991-13314010 | None:intergenic | 40.0% | |
| TTGTTCTTCCATGATCCAGA+TGG | + | chr8.3:13314275-13314294 | MS.gene61492:CDS | 40.0% | |
| ! | TCTCTGATAGAGGTACAACT+GGG | - | chr8.3:13314340-13314359 | None:intergenic | 40.0% |
| ! | TTCTCTGATAGAGGTACAAC+TGG | - | chr8.3:13314341-13314360 | None:intergenic | 40.0% |
| !! | ATTTCAATCATGGTGCCATC+TGG | - | chr8.3:13314293-13314312 | None:intergenic | 40.0% |
| AAGGAGGTAGGCCTCTTAAT+AGG | - | chr8.3:13313759-13313778 | None:intergenic | 45.0% | |
| ! | GTGTGAAAGTATGGTAGCAG+TGG | + | chr8.3:13314178-13314197 | MS.gene61492:intron | 45.0% |
| CCATGCACCATTGAAGGCTA+AGG | - | chr8.3:13313778-13313797 | None:intergenic | 50.0% | |
| CCATTGAAGGCTAAGGAGGT+AGG | - | chr8.3:13313771-13313790 | None:intergenic | 50.0% | |
| CTGGGTCATCTGATTGGAGA+AGG | - | chr8.3:13313985-13314004 | None:intergenic | 50.0% | |
| TGCACCATTGAAGGCTAAGG+AGG | - | chr8.3:13313775-13313794 | None:intergenic | 50.0% | |
| ! | CCTACCTCCTTAGCCTTCAA+TGG | + | chr8.3:13313768-13313787 | MS.gene61492:CDS | 50.0% |
| ! | CCTTAGCCTTCAATGGTGCA+TGG | + | chr8.3:13313775-13313794 | MS.gene61492:CDS | 50.0% |
| ! | GATAGAGGTACAACTGGGTC+TGG | - | chr8.3:13314335-13314354 | None:intergenic | 50.0% |
| !! | TCATGGTGCCATCTGGATCA+TGG | - | chr8.3:13314286-13314305 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 13313654 | 13314439 | 13313654 | ID=MS.gene61492 |
| chr8.3 | mRNA | 13313654 | 13314439 | 13313654 | ID=MS.gene61492.t1;Parent=MS.gene61492 |
| chr8.3 | exon | 13313654 | 13313796 | 13313654 | ID=MS.gene61492.t1.exon1;Parent=MS.gene61492.t1 |
| chr8.3 | CDS | 13313654 | 13313796 | 13313654 | ID=cds.MS.gene61492.t1;Parent=MS.gene61492.t1 |
| chr8.3 | exon | 13313955 | 13314060 | 13313955 | ID=MS.gene61492.t1.exon2;Parent=MS.gene61492.t1 |
| chr8.3 | CDS | 13313955 | 13314060 | 13313955 | ID=cds.MS.gene61492.t1;Parent=MS.gene61492.t1 |
| chr8.3 | exon | 13314179 | 13314439 | 13314179 | ID=MS.gene61492.t1.exon3;Parent=MS.gene61492.t1 |
| chr8.3 | CDS | 13314179 | 13314439 | 13314179 | ID=cds.MS.gene61492.t1;Parent=MS.gene61492.t1 |
| Gene Sequence |
| Protein sequence |
